Tan0020610 (gene) Snake gourd v1

Overview
NameTan0020610
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptioncaffeoyl-CoA O-methyltransferase-like
LocationLG04: 478879 .. 481785 (-)
RNA-Seq ExpressionTan0020610
SyntenyTan0020610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAGCTGCTATATTATAAACAACGCGCAGTATGCCAAAAATGCTCACACTTATTTGCAAGCATCCAAGCACGTTCGTTGCAGAGTAGGTCATGGCCGACAATTTTCCCAAGAACATCCTCCAGAGCACTGCTCTTCTCCAGGTTCGTTCCCATTCCAATCTGAATCTTGTTCTTGACTTCATTTCAATTCCGAGTACCAATTAATCCATGGACTGCGCCTTAATTAGCGATTGAACTCAGATTAATTAACTTGATGCTTGAATTTAATCAAACACTGTTAGGAAAAAATGTCTGAAGTTAATTGAGAAAAGTTGAAAACACGTGTTTGATTGCAGTATATACTGGAAGCCAATGCCTATCCGAGAGAGCACGAACAATTGAAGGAACTCAGAGAAACCACCTTCGGAAAGTTCGACAAGTCCCGGTGAGTTTTCGATTCTTCTTCGGCATTCAAACTTTTCCGATTCGATTTTAGAATGATCGATTTAGTCCGATTCAGTCGATTGAGTCAATCGATCGACTCGGAAGGATTTAGAATGATCGATTTGGATGGATTGAATTCGATAATTGTGTAAGAGACTTAATATTTGGAATGAAATTTGGAGATTGAGTTGCAGGAGCGCGATGTGTATACCGGCGGATCAAGGATTGTTTCTATCAATGCTTCTGAAACTGATGAATGCAAAGAAAACGATAGAGATCGGCGTATTCACTGGCTATTCGCTCCTAACCACTGCGCTTGCACTGCCCGTTGATGGCCAGGTAAGGCTTAGTTTCCCCTCCATCTTCATCGTGATCGTCATCAACTTAATTAATTTTCATTCAATTACATACGATTGGCAAATAGATTTGTGATTTCTTTTTATTTTTATTTTTTTATATATATACTTTCATTCATTATCGAGTTAAGTAACAAAGTTTGAGATTGAGATTGTGCTTAATACATTTGGATATTTGATATATGTTTTTTTAGAACATGTTTGATATATGTTTGATATATATACTTATTAATTTTCAATATAAACTCATATATTTTGAAATTTTTAAATAAGTTATTGGACTCTCAATTTATTACTTCAAATTTGTAATTACAACATAAGTTGATTTCAAATTGACTATTTGGTGAGGTAAGTGTTAAACTAAATACTTATTAGATGTTTTATTAAAAGTTTATGAGTTTGTTACACATTTTTTATTCTAAAAAAATCTAATTAACACTAAACTTAAAGATGGAAAATCTATTAGAATATTTTTAAATTATATGATGAATTAGAAGGACGGGTGGATTGATTGGACATAATTTGAAAGAATTACCCAAATTTTAATTTATCTTTATTGTTTTATTTGTTTATTTATTTGGGTGATAGATAATAGCTATTGATCCAGATAGAGAATCATTTGAGATTGGTTTGCCATTTATCAAAAAGGCAGGAGTTGATCACAAGATCAAATTTCTAGAATCTCAAGCATTGGTTGTCCTGAATGACCTCCTCAATGATGTAAGTTATTGATGGAAAGTTGCGTTTTAAAGATAGTTTTCTATCTTATAATATAAATTTAGCAAAAGTGTAAAACTATATTTAGTTTTCTAGTTGTAGAATGACATTCTTTTAAACATTTTTTTCTTACCTACACTACAATAGATCTGTATTGATAAGTTGTCTTTTGAACATAGTTTTCTATTTTATATTATCAATTTGGTAAAAGCGTAACCCTATATTTAGTTTTCTAGTTTTAGAATGACATTCTTTTAAACATTTTTTTTCTTACATACAAACACAATTGATTATAGATCTATAATAATATTTTATATTATCTCCACCCAATTCTTATTCAACATTTAAAATGTATTTTTTTTCTATAGGTAGAAAGTTCAAGTCTTTACTTTAGATTTTTAATATTATATTCTTGAAAAGTTAATTTGATAGTATTTACAATTAAGTAGAAATATTTAGATATATTTAAACTTAAATTTTTTTAAAAATTAACTTGTGAAATATGGAAACTTGAACCTTCAATCTCAAAAAGAAGAAATATATGCAACTGTGCTCACTTTGATTAAAATTTAACTCTTTCAAATCTTGCAATATTGACTACTAACTTTATTTTTATTATTATTTATTTTATTGAACATGCAACAATAATATAGAGGAAAAGTCATAAGTGATGAAATGAGCTAGCATAACTCAATTAGCATAAAGTAACACTAAAAAGTTAGAGTTTCAAATGTTCCACTTCACATATTGTTGAACTAAAAAAACTATTATTAAACAACTCAAAAAGGGTTAAAGAAAAAGTCATCCTAAAGTTGAAGGTATTTTCTAATTTCTAGTATATCTAATAATTACACGTCTAACTATTAATAGATATAGTACAGTCGAAAAAGATTTCTCTTATGAAGTAAGATGATATTATTATTATTATTATTTTAATTCATTTTATATATACAGGAGAAAGAAGGAGAGTTTGATTTTGCATTTGTGGATGCTAAGAAAGTAGAGTACATTAAATATCATGAGTTGCTTCTAAAATTGGTGAAGGTAGGTGGAGTAATTGCCTATGATAACACTCTGTGGTTTGGATCTGTGGCACTGAGCGACGACGTCGTTGAGGAAGTGTTGAAGGCAAATAGAATTCTTACACGAGAATTCAATGCTTTTCTAGTCAATGACTCACGCGTGGAGATAACTCTCCTCTCCATTGGAGATGGCTTCACTCTCTGCAGGCGTATTAAGTAATTTGTTGAGTCTTTCTATCTTCTAAATTCTCACAAGATCGAGACTATTGTCTTAATAATTAGCCCAAAGGCGGTTGTTTTTAAGAAATAGCTATCGATTATGAAATTCATGGTCGATCAAAGTCCTCAATGGATTTGAATAGATACATAATTTCATATATTGCCATAAA

mRNA sequence

GGAGCTGCTATATTATAAACAACGCGCAGTATGCCAAAAATGCTCACACTTATTTGCAAGCATCCAAGCACGTTCGTTGCAGAGTAGGTCATGGCCGACAATTTTCCCAAGAACATCCTCCAGAGCACTGCTCTTCTCCAGTATATACTGGAAGCCAATGCCTATCCGAGAGAGCACGAACAATTGAAGGAACTCAGAGAAACCACCTTCGGAAAGTTCGACAAGTCCCGGAGCGCGATGTGTATACCGGCGGATCAAGGATTGTTTCTATCAATGCTTCTGAAACTGATGAATGCAAAGAAAACGATAGAGATCGGCGTATTCACTGGCTATTCGCTCCTAACCACTGCGCTTGCACTGCCCGTTGATGGCCAGATAATAGCTATTGATCCAGATAGAGAATCATTTGAGATTGGTTTGCCATTTATCAAAAAGGCAGGAGTTGATCACAAGATCAAATTTCTAGAATCTCAAGCATTGGTTGTCCTGAATGACCTCCTCAATGATGAGAAAGAAGGAGAGTTTGATTTTGCATTTGTGGATGCTAAGAAAGTAGAGTACATTAAATATCATGAGTTGCTTCTAAAATTGGTGAAGGTAGGTGGAGTAATTGCCTATGATAACACTCTGTGGTTTGGATCTGTGGCACTGAGCGACGACGTCGTTGAGGAAGTGTTGAAGGCAAATAGAATTCTTACACGAGAATTCAATGCTTTTCTAGTCAATGACTCACGCGTGGAGATAACTCTCCTCTCCATTGGAGATGGCTTCACTCTCTGCAGGCGTATTAAGTAATTTGTTGAGTCTTTCTATCTTCTAAATTCTCACAAGATCGAGACTATTGTCTTAATAATTAGCCCAAAGGCGGTTGTTTTTAAGAAATAGCTATCGATTATGAAATTCATGGTCGATCAAAGTCCTCAATGGATTTGAATAGATACATAATTTCATATATTGCCATAAA

Coding sequence (CDS)

ATGGCCGACAATTTTCCCAAGAACATCCTCCAGAGCACTGCTCTTCTCCAGTATATACTGGAAGCCAATGCCTATCCGAGAGAGCACGAACAATTGAAGGAACTCAGAGAAACCACCTTCGGAAAGTTCGACAAGTCCCGGAGCGCGATGTGTATACCGGCGGATCAAGGATTGTTTCTATCAATGCTTCTGAAACTGATGAATGCAAAGAAAACGATAGAGATCGGCGTATTCACTGGCTATTCGCTCCTAACCACTGCGCTTGCACTGCCCGTTGATGGCCAGATAATAGCTATTGATCCAGATAGAGAATCATTTGAGATTGGTTTGCCATTTATCAAAAAGGCAGGAGTTGATCACAAGATCAAATTTCTAGAATCTCAAGCATTGGTTGTCCTGAATGACCTCCTCAATGATGAGAAAGAAGGAGAGTTTGATTTTGCATTTGTGGATGCTAAGAAAGTAGAGTACATTAAATATCATGAGTTGCTTCTAAAATTGGTGAAGGTAGGTGGAGTAATTGCCTATGATAACACTCTGTGGTTTGGATCTGTGGCACTGAGCGACGACGTCGTTGAGGAAGTGTTGAAGGCAAATAGAATTCTTACACGAGAATTCAATGCTTTTCTAGTCAATGACTCACGCGTGGAGATAACTCTCCTCTCCATTGGAGATGGCTTCACTCTCTGCAGGCGTATTAAGTAA

Protein sequence

MADNFPKNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLFLSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDHKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNTLWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRIK
Homology
BLAST of Tan0020610 vs. ExPASy Swiss-Prot
Match: C7AE94 (Flavonoid 3',5'-methyltransferase OS=Vitis vinifera OX=29760 GN=FAOMT PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-75
Identity = 147/237 (62.03%), Postives = 186/237 (78.48%), Query Frame = 0

Query: 1   MADNFPKNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLFL 60
           M+ +  + IL++ AL +Y+LE +AYPREHEQLK LRE T  K  K  S M +P D+GLF+
Sbjct: 1   MSSSSHRGILKTEALTKYLLETSAYPREHEQLKGLREATVEK-HKYWSLMNVPVDEGLFI 60

Query: 61  SMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDH 120
           SMLLK+MNAKKTIE+GVFTGYSLL TALALP DG+IIA+DPD+E+++ G+PFIKKAGV+H
Sbjct: 61  SMLLKIMNAKKTIELGVFTGYSLLATALALPQDGKIIAVDPDKEAYQTGVPFIKKAGVEH 120

Query: 121 KIKFLESQALVVLNDLLNDEK-EGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           KI F++S A+ VLNDL+ D K EG  DFA VDA K  Y+ YHELLLKLV+VGG+IAYDNT
Sbjct: 121 KINFIQSDAMSVLNDLIADGKEEGTLDFAMVDADKENYLNYHELLLKLVRVGGIIAYDNT 180

Query: 181 LWFGSVALSDDVVEEVL---KANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
           LWFGSVA S++  EE++   +A R+   + N FL +D RVE++ LSIGDG  LCRR+
Sbjct: 181 LWFGSVARSEE--EEMMDFERAGRVHLMKLNKFLASDPRVELSHLSIGDGVALCRRL 234

BLAST of Tan0020610 vs. ExPASy Swiss-Prot
Match: P93711 (Caffeoyl-CoA O-methyltransferase OS=Populus kitakamiensis OX=34292 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 4.9e-75
Identity = 147/228 (64.47%), Postives = 178/228 (78.07%), Query Frame = 0

Query: 7   KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLFLSMLLKL 66
           K ILQS AL QYI E +AYP EHEQLKELRE T  K+  S S M +P D+G FLSMLLKL
Sbjct: 8   KGILQSEALKQYIYETSAYPGEHEQLKELREATTKKYG-SLSGMSVPVDEGRFLSMLLKL 67

Query: 67  MNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDHKIKFLE 126
           MNAK+T+E+GVFTGYSLL+TALALP DGQ+ AID DR ++EIGLPFI+KAGV+ KI F++
Sbjct: 68  MNAKRTLEVGVFTGYSLLSTALALPEDGQVTAIDKDRGAYEIGLPFIQKAGVEDKINFIQ 127

Query: 127 SQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNTLWFGSVA 186
           S+A  +LN++L ++K+ EFDFAFVDA K  Y  YHE LLKLVK+GG+IAYDNTLW+G VA
Sbjct: 128 SEAPPILNEMLCNDKQPEFDFAFVDADKSSYKHYHEQLLKLVKIGGIIAYDNTLWYGLVA 187

Query: 187 LS-DDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
              DD V E L+  R +  EFN  L +D RVEI+ +SIGDG TLCRR+
Sbjct: 188 KEVDDEVPEPLRMVRTVIMEFNKLLSSDLRVEISQISIGDGVTLCRRL 234

BLAST of Tan0020610 vs. ExPASy Swiss-Prot
Match: Q9C9W3 (Putative caffeoyl-CoA O-methyltransferase At1g67980 OS=Arabidopsis thaliana OX=3702 GN=At1g67980 PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 2.9e-72
Identity = 145/234 (61.97%), Postives = 176/234 (75.21%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MA+  P K IL+S AL QYI+E +AYPREHE LKELR+ T  K+  + S M +P D+G F
Sbjct: 1   MANEIPTKGILKSEALKQYIMETSAYPREHELLKELRKATVQKYG-NLSEMEVPVDEGHF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           LSML+K+MNAK TIEIGVFTGYSLLTTALALP DG+I AID D+E++E+GL FIKKAGVD
Sbjct: 61  LSMLVKIMNAKNTIEIGVFTGYSLLTTALALPEDGRITAIDIDKEAYEVGLEFIKKAGVD 120

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ S  L  L+ L+ND  + EFDFAF DA K  Y+ +HE LLKLVKVGG+IA+DNT
Sbjct: 121 HKINFIHSDGLKALDQLVND--KCEFDFAFADADKSSYVNFHERLLKLVKVGGIIAFDNT 180

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
           LWFG VA  +D V E ++  R    EFN  L  D RVE++ +SIGDG TLCRR+
Sbjct: 181 LWFGFVAEDEDGVPEHMREYRAALIEFNKKLALDPRVEVSQISIGDGITLCRRL 231

BLAST of Tan0020610 vs. ExPASy Swiss-Prot
Match: A0A077EW86 (Norbelladine 4'-O-methyltransferase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=N4OMT2 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.3e-69
Identity = 144/233 (61.80%), Postives = 175/233 (75.11%), Query Frame = 0

Query: 7   KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSR-SAMCIPADQGLFLSMLLK 66
           KNIL S  LL+YILE +AYPREHEQLK LRE T    +K   S+  +PAD+GLFLSMLLK
Sbjct: 13  KNILHSEDLLKYILETSAYPREHEQLKGLREVT----EKHEWSSALVPADEGLFLSMLLK 72

Query: 67  LMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDHKIKFL 126
           LMNAK+TIEIGV+TGYSLLTTALALP DG+I AID ++  FEIGLPFI+KAGV+HKI F+
Sbjct: 73  LMNAKRTIEIGVYTGYSLLTTALALPEDGKITAIDVNKSFFEIGLPFIQKAGVEHKINFI 132

Query: 127 ESQALVVLNDLLNDEKEGE-FDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNTLWFGS 186
           ES+AL VL+ +L + KE + +D+AFVDA K  Y  YHE L+KLV++GG I YDNTLW+GS
Sbjct: 133 ESEALPVLDQMLQETKEEDLYDYAFVDADKSNYANYHERLVKLVRIGGAILYDNTLWYGS 192

Query: 187 VALSD----DVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
           VA  +       EEV    R+  R  N FL  D RVEI+ +SIGDG T+CRR+
Sbjct: 193 VAYPEYPGLHPEEEVA---RLSFRNLNTFLAADPRVEISQVSIGDGVTICRRL 238

BLAST of Tan0020610 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ6 (Norbelladine 4'-O-methyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=N4OMT PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 4.0e-69
Identity = 141/230 (61.30%), Postives = 175/230 (76.09%), Query Frame = 0

Query: 7   KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSR-SAMCIPADQGLFLSMLLK 66
           KNIL S  LL+YILE +AYPREHEQLK LRE T    +K   S+  +PAD+GLFLSMLLK
Sbjct: 13  KNILHSEDLLKYILETSAYPREHEQLKGLREVT----EKHEWSSALVPADEGLFLSMLLK 72

Query: 67  LMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDHKIKFL 126
           LMNAK+TIEIGV+TGYSLLTTALALP DG+I AID ++  FEIGLPFI+KAGV+HKI F+
Sbjct: 73  LMNAKRTIEIGVYTGYSLLTTALALPEDGKITAIDVNKSFFEIGLPFIQKAGVEHKINFI 132

Query: 127 ESQALVVLNDLLNDEKEGE-FDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNTLWFGS 186
           ES+AL VL+ +L + KE + +D+AFVDA K  Y  YHE L+KLV++GG I YDNTLW+GS
Sbjct: 133 ESEALPVLDQMLQEMKEEDLYDYAFVDADKSNYANYHERLVKLVRIGGAILYDNTLWYGS 192

Query: 187 VALSD-DVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
           VA  +   +    + +R+  R  N FL  D RVEI+ +SIGDG T+CRR+
Sbjct: 193 VAYPEYPGLYPEDEVDRLSFRNLNTFLAADPRVEISQVSIGDGVTICRRL 238

BLAST of Tan0020610 vs. NCBI nr
Match: XP_022151835.1 (flavonoid 3',5'-methyltransferase-like [Momordica charantia])

HSP 1 Score: 383.3 bits (983), Expect = 1.5e-102
Identity = 197/234 (84.19%), Postives = 212/234 (90.60%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MA++FP KNILQS ALLQYILE+NAYPREHEQLKELRETT GKFDKSRS M +PADQGLF
Sbjct: 18  MANDFPDKNILQSPALLQYILESNAYPREHEQLKELRETTVGKFDKSRSVMGVPADQGLF 77

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           L+ML+KLMNAKKTIEIGVFTGYSLLTTALALP DGQI AID DR+SFEIGLPFIKKAGVD
Sbjct: 78  LAMLVKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVDRKSFEIGLPFIKKAGVD 137

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ES+AL+VLNDLL D KEGEFDFAFVDAKK EYIKYHELLL+LVKVGGVI YDNT
Sbjct: 138 HKINFVESEALIVLNDLLKDGKEGEFDFAFVDAKKSEYIKYHELLLRLVKVGGVIGYDNT 197

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
           LW+GSVA S+++VE  LK NRI  RE NAFLVNDSRVEI LLSIGDG TLCRRI
Sbjct: 198 LWYGSVAQSEELVEADLKDNRIYMRELNAFLVNDSRVEIALLSIGDGLTLCRRI 251

BLAST of Tan0020610 vs. NCBI nr
Match: XP_022966735.1 (caffeoyl-CoA O-methyltransferase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 378.6 bits (971), Expect = 3.8e-101
Identity = 197/235 (83.83%), Postives = 208/235 (88.51%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MAD+ P KNILQSTALLQYILEAN+YPREHEQLKELRETT  KFDKS S M +PADQGLF
Sbjct: 1   MADDCPDKNILQSTALLQYILEANSYPREHEQLKELRETTLRKFDKSLSVMSVPADQGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           LSMLLKLMNAKKTIEIGVFTGYSLLTTALALP DGQI AID +RESFEIGLPF KKAGVD
Sbjct: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRESFEIGLPFFKKAGVD 120

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ES ALVVLNDL+ND+   EFDFAFVDA K EYIKYHELLL+LVKVGG+IAYDNT
Sbjct: 121 HKINFVESMALVVLNDLINDDTNTEFDFAFVDAVKGEYIKYHELLLRLVKVGGIIAYDNT 180

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRIK 235
           LW+GSVA +DDVV   LKANR   REFNAFLVNDSRVEI LLSIGDG TLCRRIK
Sbjct: 181 LWYGSVAQNDDVVPHDLKANRSHIREFNAFLVNDSRVEIALLSIGDGLTLCRRIK 235

BLAST of Tan0020610 vs. NCBI nr
Match: XP_022945592.1 (caffeoyl-CoA O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 377.9 bits (969), Expect = 6.5e-101
Identity = 195/235 (82.98%), Postives = 210/235 (89.36%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MAD+ P KNILQSTALLQYILEAN+YPREHEQLKELRETT  KFDKS S M +P DQGLF
Sbjct: 1   MADDCPDKNILQSTALLQYILEANSYPREHEQLKELRETTLRKFDKSLSVMSVPGDQGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           LSMLLKLMNAKKTIEIGVFTGYSLLTTALALP DGQI AID +R SFEIGLPFIKKAGVD
Sbjct: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRASFEIGLPFIKKAGVD 120

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ESQAL+VLN+L+ND+ + EFDFAFVDA K EYIKYHE LL+LVKVGGVIAYDNT
Sbjct: 121 HKINFVESQALIVLNNLINDDAKTEFDFAFVDAVKGEYIKYHEFLLRLVKVGGVIAYDNT 180

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRIK 235
           LW+GSVA SDDVV++ LKANR   REFNAFLVNDSRVEI LLSIGDG TLCRR+K
Sbjct: 181 LWYGSVAQSDDVVQDDLKANRSHIREFNAFLVNDSRVEIALLSIGDGLTLCRRVK 235

BLAST of Tan0020610 vs. NCBI nr
Match: KAG6573644.1 (hypothetical protein SDJN03_27531, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.1 bits (967), Expect = 1.1e-100
Identity = 195/235 (82.98%), Postives = 210/235 (89.36%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MAD+ P KNILQSTALLQYILEAN+YPREHEQLKELRETT  KFDKS S M +PADQGLF
Sbjct: 1   MADDCPDKNILQSTALLQYILEANSYPREHEQLKELRETTLRKFDKSLSVMSVPADQGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           LSMLLKLMNAKKTIEIGVFTGYSLLTTALALP DGQI AID +R SFEIGLPFIKKAGVD
Sbjct: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRASFEIGLPFIKKAGVD 120

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ESQAL+VLN+L+ND+ + EFDFAFVDA K EYIKYHE LL+LVKVGGVIAYDNT
Sbjct: 121 HKINFVESQALIVLNNLINDDTKTEFDFAFVDAVKGEYIKYHEFLLRLVKVGGVIAYDNT 180

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRIK 235
           LW+GS+A SDDVV + LKANR   REFNAFLVNDSRVEI LLSIGDG TLCRR+K
Sbjct: 181 LWYGSLAQSDDVVPDDLKANRSHIREFNAFLVNDSRVEIALLSIGDGLTLCRRVK 235

BLAST of Tan0020610 vs. NCBI nr
Match: XP_023541696.1 (caffeoyl-CoA O-methyltransferase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375.9 bits (964), Expect = 2.5e-100
Identity = 194/235 (82.55%), Postives = 210/235 (89.36%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MAD+ P KNILQSTALLQYILEAN+YPREHEQLKELRETT  KFDKS S M +PADQGLF
Sbjct: 1   MADDCPDKNILQSTALLQYILEANSYPREHEQLKELRETTLRKFDKSLSVMSVPADQGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           LSMLLKLMNAKKTIEIGVFTGYSLLTTALALP DGQI AID +R SFEIGLPFIKKAGVD
Sbjct: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRASFEIGLPFIKKAGVD 120

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ESQAL+VLN+L+ND+ + E+DFAFVDA K EYIKYHE LL+LVKVGGVIAYDNT
Sbjct: 121 HKINFVESQALIVLNNLINDDTKTEYDFAFVDAVKGEYIKYHEFLLRLVKVGGVIAYDNT 180

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRIK 235
           LW+GSVA +DDVV + LKANR   REFNAFLVNDSRVEI LLSIGDG TLCRR+K
Sbjct: 181 LWYGSVAQNDDVVPDDLKANRSHIREFNAFLVNDSRVEIALLSIGDGLTLCRRVK 235

BLAST of Tan0020610 vs. ExPASy TrEMBL
Match: A0A6J1DCA7 (flavonoid 3',5'-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111019721 PE=3 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 7.5e-103
Identity = 197/234 (84.19%), Postives = 212/234 (90.60%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MA++FP KNILQS ALLQYILE+NAYPREHEQLKELRETT GKFDKSRS M +PADQGLF
Sbjct: 18  MANDFPDKNILQSPALLQYILESNAYPREHEQLKELRETTVGKFDKSRSVMGVPADQGLF 77

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           L+ML+KLMNAKKTIEIGVFTGYSLLTTALALP DGQI AID DR+SFEIGLPFIKKAGVD
Sbjct: 78  LAMLVKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVDRKSFEIGLPFIKKAGVD 137

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ES+AL+VLNDLL D KEGEFDFAFVDAKK EYIKYHELLL+LVKVGGVI YDNT
Sbjct: 138 HKINFVESEALIVLNDLLKDGKEGEFDFAFVDAKKSEYIKYHELLLRLVKVGGVIGYDNT 197

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
           LW+GSVA S+++VE  LK NRI  RE NAFLVNDSRVEI LLSIGDG TLCRRI
Sbjct: 198 LWYGSVAQSEELVEADLKDNRIYMRELNAFLVNDSRVEIALLSIGDGLTLCRRI 251

BLAST of Tan0020610 vs. ExPASy TrEMBL
Match: A0A6J1HSF7 (caffeoyl-CoA O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466357 PE=3 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.8e-101
Identity = 197/235 (83.83%), Postives = 208/235 (88.51%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MAD+ P KNILQSTALLQYILEAN+YPREHEQLKELRETT  KFDKS S M +PADQGLF
Sbjct: 1   MADDCPDKNILQSTALLQYILEANSYPREHEQLKELRETTLRKFDKSLSVMSVPADQGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           LSMLLKLMNAKKTIEIGVFTGYSLLTTALALP DGQI AID +RESFEIGLPF KKAGVD
Sbjct: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRESFEIGLPFFKKAGVD 120

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ES ALVVLNDL+ND+   EFDFAFVDA K EYIKYHELLL+LVKVGG+IAYDNT
Sbjct: 121 HKINFVESMALVVLNDLINDDTNTEFDFAFVDAVKGEYIKYHELLLRLVKVGGIIAYDNT 180

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRIK 235
           LW+GSVA +DDVV   LKANR   REFNAFLVNDSRVEI LLSIGDG TLCRRIK
Sbjct: 181 LWYGSVAQNDDVVPHDLKANRSHIREFNAFLVNDSRVEIALLSIGDGLTLCRRIK 235

BLAST of Tan0020610 vs. ExPASy TrEMBL
Match: A0A6J1G1C7 (caffeoyl-CoA O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111449784 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 3.1e-101
Identity = 195/235 (82.98%), Postives = 210/235 (89.36%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MAD+ P KNILQSTALLQYILEAN+YPREHEQLKELRETT  KFDKS S M +P DQGLF
Sbjct: 1   MADDCPDKNILQSTALLQYILEANSYPREHEQLKELRETTLRKFDKSLSVMSVPGDQGLF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           LSMLLKLMNAKKTIEIGVFTGYSLLTTALALP DGQI AID +R SFEIGLPFIKKAGVD
Sbjct: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDVNRASFEIGLPFIKKAGVD 120

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ESQAL+VLN+L+ND+ + EFDFAFVDA K EYIKYHE LL+LVKVGGVIAYDNT
Sbjct: 121 HKINFVESQALIVLNNLINDDAKTEFDFAFVDAVKGEYIKYHEFLLRLVKVGGVIAYDNT 180

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRIK 235
           LW+GSVA SDDVV++ LKANR   REFNAFLVNDSRVEI LLSIGDG TLCRR+K
Sbjct: 181 LWYGSVAQSDDVVQDDLKANRSHIREFNAFLVNDSRVEIALLSIGDGLTLCRRVK 235

BLAST of Tan0020610 vs. ExPASy TrEMBL
Match: A0A6J1G1B9 (flavonoid 3',5'-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449785 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 7.7e-100
Identity = 194/234 (82.91%), Postives = 206/234 (88.03%), Query Frame = 0

Query: 1   MADNFPKNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLFL 60
           MAD+  KNILQ  ALLQYILE N+YPREHEQLKELRETT   F KSRS M +PADQGLFL
Sbjct: 1   MADDSKKNILQCPALLQYILEENSYPREHEQLKELRETTLETF-KSRSVMSVPADQGLFL 60

Query: 61  SMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDH 120
           SMLLKLMNAKKTIEIGVFTGYSLLTTALALP DGQI AID  RESFE+GLPFIKKAGVDH
Sbjct: 61  SMLLKLMNAKKTIEIGVFTGYSLLTTALALPADGQITAIDISRESFEVGLPFIKKAGVDH 120

Query: 121 KIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNTL 180
           KI F+ESQAL+VLNDLLND KEGEFDF FVDA K EYIKYHELL+KLVKVGGVIAYDNTL
Sbjct: 121 KINFVESQALIVLNDLLNDGKEGEFDFVFVDADKGEYIKYHELLMKLVKVGGVIAYDNTL 180

Query: 181 WFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRIK 235
           WFG+VA SDDVV+E LKA+R   RE NAFLVNDSRVEI LLS+GDG TLCRRI+
Sbjct: 181 WFGTVAQSDDVVDEKLKASRNHIRELNAFLVNDSRVEIALLSVGDGITLCRRIE 233

BLAST of Tan0020610 vs. ExPASy TrEMBL
Match: A0A6J1G1E5 (flavonoid 3',5'-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449785 PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.9e-98
Identity = 191/234 (81.62%), Postives = 205/234 (87.61%), Query Frame = 0

Query: 1   MADNFPKNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLFL 60
           MAD+  KNILQ  ALLQYILE N+YPREHEQLKELRETT   F K  S M +PADQGLFL
Sbjct: 1   MADDSKKNILQCPALLQYILEENSYPREHEQLKELRETTLETF-KYWSLMSVPADQGLFL 60

Query: 61  SMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDH 120
           SMLLKLMNAKKT+EIGVFTGYSLLTTALALP DGQIIA+D +R+SFEIGLPFIKKAGVDH
Sbjct: 61  SMLLKLMNAKKTLEIGVFTGYSLLTTALALPADGQIIAVDVNRDSFEIGLPFIKKAGVDH 120

Query: 121 KIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNTL 180
           KI F+ES  + VLNDLLND KEGEFDFAFVDA K EYIKYHELLLKLVK+GG+IAYDNTL
Sbjct: 121 KINFVESHGMPVLNDLLNDGKEGEFDFAFVDANKDEYIKYHELLLKLVKIGGIIAYDNTL 180

Query: 181 WFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRIK 235
           WFG+VA SDDVVEE LKA+R  TRE NAFLVNDSRVEI LLS+GDG TLCRRIK
Sbjct: 181 WFGTVAQSDDVVEEKLKASRGHTRELNAFLVNDSRVEIALLSVGDGLTLCRRIK 233

BLAST of Tan0020610 vs. TAIR 10
Match: AT1G67980.1 (caffeoyl-CoA 3-O-methyltransferase )

HSP 1 Score: 273.1 bits (697), Expect = 2.1e-73
Identity = 145/234 (61.97%), Postives = 176/234 (75.21%), Query Frame = 0

Query: 1   MADNFP-KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLF 60
           MA+  P K IL+S AL QYI+E +AYPREHE LKELR+ T  K+  + S M +P D+G F
Sbjct: 1   MANEIPTKGILKSEALKQYIMETSAYPREHELLKELRKATVQKYG-NLSEMEVPVDEGHF 60

Query: 61  LSMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVD 120
           LSML+K+MNAK TIEIGVFTGYSLLTTALALP DG+I AID D+E++E+GL FIKKAGVD
Sbjct: 61  LSMLVKIMNAKNTIEIGVFTGYSLLTTALALPEDGRITAIDIDKEAYEVGLEFIKKAGVD 120

Query: 121 HKIKFLESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           HKI F+ S  L  L+ L+ND  + EFDFAF DA K  Y+ +HE LLKLVKVGG+IA+DNT
Sbjct: 121 HKINFIHSDGLKALDQLVND--KCEFDFAFADADKSSYVNFHERLLKLVKVGGIIAFDNT 180

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
           LWFG VA  +D V E ++  R    EFN  L  D RVE++ +SIGDG TLCRR+
Sbjct: 181 LWFGFVAEDEDGVPEHMREYRAALIEFNKKLALDPRVEVSQISIGDGITLCRRL 231

BLAST of Tan0020610 vs. TAIR 10
Match: AT1G67990.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 5.3e-69
Identity = 135/227 (59.47%), Postives = 168/227 (74.01%), Query Frame = 0

Query: 7   KNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLFLSMLLKL 66
           K IL+S AL QYI+E  AYPREHE LKELRE T  ++  + S M +P D+ LFLSML+K+
Sbjct: 8   KGILKSEALKQYIMETTAYPREHELLKELREATIQRYG-NLSEMGVPVDESLFLSMLVKI 67

Query: 67  MNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDHKIKFLE 126
           +NAK TIEIGVFTGYSL T ALALP DG+I AID D+  + +GL F+KKAGVDHKI F++
Sbjct: 68  INAKNTIEIGVFTGYSLFTVALALPEDGRITAIDIDQAGYNLGLEFMKKAGVDHKINFIQ 127

Query: 127 SQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNTLWFGSVA 186
           S A+  L+ LLN EK+ E+DFAFVDA K  Y+ + E LLKLVKVGG+IA+DNTLWFG++ 
Sbjct: 128 SDAVRGLDQLLNGEKQ-EYDFAFVDADKTNYVYFLEKLLKLVKVGGIIAFDNTLWFGTLI 187

Query: 187 LSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
             ++ V   ++A R    EFN  L  D RVEI  +SIGDG TLCRR+
Sbjct: 188 QKENEVPGHMRAYREALLEFNKILARDPRVEIAQISIGDGLTLCRRL 232

BLAST of Tan0020610 vs. TAIR 10
Match: AT1G24735.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 256.9 bits (655), Expect = 1.6e-68
Identity = 134/228 (58.77%), Postives = 166/228 (72.81%), Query Frame = 0

Query: 6   PKNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLFLSMLLK 65
           PK IL+S AL +YI E  AYPREHEQLK+LRE T  K+  + S M +P D+G FLSMLLK
Sbjct: 13  PKGILKSDALKKYIFETTAYPREHEQLKKLREATVLKYG-NLSEMEVPVDEGHFLSMLLK 72

Query: 66  LMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDHKIKFL 125
           +MNAKKTIE+GVFTGYSLLTTALALP DG +  ID D+E++E+GL FIK AGV HKI F+
Sbjct: 73  IMNAKKTIELGVFTGYSLLTTALALPHDGHVTGIDIDKEAYEMGLEFIKNAGVHHKINFI 132

Query: 126 ESQALVVLNDLLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNTLWFGSV 185
            S  L  L+++L++  + EFDFAFVDA K  Y   HE L+KLVKVGGVIA+DNTLW G V
Sbjct: 133 HSDCLQALDNMLSENPKPEFDFAFVDADKPNYANMHERLMKLVKVGGVIAFDNTLWSGFV 192

Query: 186 ALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
           A  ++ V   ++ NR    + N  L  D  VE++ +SIGDG TLCRR+
Sbjct: 193 AEKEENVPVHMRVNRKAFLDLNKRLAADPHVEVSQVSIGDGVTLCRRL 239

BLAST of Tan0020610 vs. TAIR 10
Match: AT1G24735.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 240.0 bits (611), Expect = 2.0e-63
Identity = 135/278 (48.56%), Postives = 166/278 (59.71%), Query Frame = 0

Query: 6   PKNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDK-------------------- 65
           PK IL+S AL +YI E  AYPREHEQLK+LRE T  K+                      
Sbjct: 13  PKGILKSDALKKYIFETTAYPREHEQLKKLREATVLKYGNLYTVLLYDHTLILSNNNCKY 72

Query: 66  ------------------------------SRSAMCIPADQGLFLSMLLKLMNAKKTIEI 125
                                          RS M +P D+G FLSMLLK+MNAKKTIE+
Sbjct: 73  HNVTTLDPTKYVYHQFKDYTSNEKMVCACMRRSEMEVPVDEGHFLSMLLKIMNAKKTIEL 132

Query: 126 GVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDHKIKFLESQALVVLND 185
           GVFTGYSLLTTALALP DG +  ID D+E++E+GL FIK AGV HKI F+ S  L  L++
Sbjct: 133 GVFTGYSLLTTALALPHDGHVTGIDIDKEAYEMGLEFIKNAGVHHKINFIHSDCLQALDN 192

Query: 186 LLNDEKEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNTLWFGSVALSDDVVEEV 234
           +L++  + EFDFAFVDA K  Y   HE L+KLVKVGGVIA+DNTLW G VA  ++ V   
Sbjct: 193 MLSENPKPEFDFAFVDADKPNYANMHERLMKLVKVGGVIAFDNTLWSGFVAEKEENVPVH 252

BLAST of Tan0020610 vs. TAIR 10
Match: AT4G26220.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 1.3e-62
Identity = 127/234 (54.27%), Postives = 163/234 (69.66%), Query Frame = 0

Query: 1   MADNFPKNILQSTALLQYILEANAYPREHEQLKELRETTFGKFDKSRSAMCIPADQGLFL 60
           MA +  K +L+S  L +YILE + YPRE E L+ELR  T    +  ++ M    D G  +
Sbjct: 1   MAKDEAKGLLKSEELYKYILETSVYPREPEVLRELRNIT---HNHPQAGMATAPDAGQLM 60

Query: 61  SMLLKLMNAKKTIEIGVFTGYSLLTTALALPVDGQIIAIDPDRESFEIGLPFIKKAGVDH 120
            MLL L+NA+KTIE+GVFTGYSLL TAL LP DG++IAID +R+S+EIGLP IKKAGV+H
Sbjct: 61  GMLLNLVNARKTIEVGVFTGYSLLLTALTLPEDGKVIAIDMNRDSYEIGLPVIKKAGVEH 120

Query: 121 KIKFLESQALVVLNDLLNDE-KEGEFDFAFVDAKKVEYIKYHELLLKLVKVGGVIAYDNT 180
           KI F ES+AL  L++LLN++  EG FDFAFVDA K+ Y  YHE L++L+KVGG+I YDNT
Sbjct: 121 KIDFKESEALPALDELLNNKVNEGGFDFAFVDADKLNYWNYHERLIRLIKVGGIIVYDNT 180

Query: 181 LWFGSVALSDDVVEEVLKANRILTREFNAFLVNDSRVEITLLSIGDGFTLCRRI 234
           LW GSVA  D    E     +  T E N  L  D RV+I+  ++GDG T+CRR+
Sbjct: 181 LWGGSVAEPDSSTPEWRIEVKKATLELNKKLSADQRVQISQAALGDGITICRRL 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C7AE941.7e-7562.03Flavonoid 3',5'-methyltransferase OS=Vitis vinifera OX=29760 GN=FAOMT PE=1 SV=1[more]
P937114.9e-7564.47Caffeoyl-CoA O-methyltransferase OS=Populus kitakamiensis OX=34292 PE=2 SV=1[more]
Q9C9W32.9e-7261.97Putative caffeoyl-CoA O-methyltransferase At1g67980 OS=Arabidopsis thaliana OX=3... [more]
A0A077EW862.3e-6961.80Norbelladine 4'-O-methyltransferase 2 OS=Narcissus aff. pseudonarcissus MK-2014 ... [more]
A0A2H5AIZ64.0e-6961.30Norbelladine 4'-O-methyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=N4O... [more]
Match NameE-valueIdentityDescription
XP_022151835.11.5e-10284.19flavonoid 3',5'-methyltransferase-like [Momordica charantia][more]
XP_022966735.13.8e-10183.83caffeoyl-CoA O-methyltransferase-like isoform X1 [Cucurbita maxima][more]
XP_022945592.16.5e-10182.98caffeoyl-CoA O-methyltransferase-like [Cucurbita moschata][more]
KAG6573644.11.1e-10082.98hypothetical protein SDJN03_27531, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023541696.12.5e-10082.55caffeoyl-CoA O-methyltransferase-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DCA77.5e-10384.19flavonoid 3',5'-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1HSF71.8e-10183.83caffeoyl-CoA O-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1G1C73.1e-10182.98caffeoyl-CoA O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111449... [more]
A0A6J1G1B97.7e-10082.91flavonoid 3',5'-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1G1E51.9e-9881.62flavonoid 3',5'-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT1G67980.12.1e-7361.97caffeoyl-CoA 3-O-methyltransferase [more]
AT1G67990.15.3e-6959.47S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G24735.11.6e-6858.77S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G24735.22.0e-6348.56S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G26220.11.3e-6254.27S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002935Class I-like SAM-dependent O-methyltransferasePFAMPF01596Methyltransf_3coord: 24..237
e-value: 4.9E-78
score: 261.1
IPR002935Class I-like SAM-dependent O-methyltransferasePROSITEPS51682SAM_OMT_Icoord: 8..237
score: 62.62339
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 2..238
e-value: 9.0E-70
score: 236.6
NoneNo IPR availablePANTHERPTHR10509:SF80FLAVONOID 3',5'-METHYLTRANSFERASE-LIKEcoord: 7..237
NoneNo IPR availablePANTHERPTHR10509O-METHYLTRANSFERASE-RELATEDcoord: 7..237
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 77..180
e-value: 0.00905133
score: 33.1723
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 15..236

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0020610.1Tan0020610.1mRNA
Tan0020610.2Tan0020610.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity