Tan0020355 (gene) Snake gourd v1

Overview
NameTan0020355
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionchitotriosidase-1-like
LocationLG08: 2854560 .. 2856679 (+)
RNA-Seq ExpressionTan0020355
SyntenyTan0020355
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCAAGGCATCATGTCCAAGCTGGTTACTGGTTCACCAGCACTGACTTCCGACTTCCCTCAGCAAATATTGATGCTAATTATTTTACTCATCTCTTTTGTGGTTATGTTGGTGTCGACGCCCAAACTTATGAAGTCGCCGTAAGTCCTTTAGAAGAGGATTTGATTTATCAATTTACTCCAACCGTCAGACTAAGCAATCATAGAGTGAAAACCCTATTGTCCATCGGAGGAGATAATGATGTGTTTGCTAAAATGGCTAGCGAGCCTACAAATCGTGATGCTTTTATTGAATCCTCCATTTCAATAGCAAGAGCAGGTGAATTTGATGGCCTCGATCTTCAATGGTTTTTTCCATCTTCTCAAGAGGAAATGGATGATTTTGAGAATCTCCTCTGTGAATGGCATAAAGCTGCTATCAAAGAAGCTAAAGAATATCATAGAACAAAATTGTTGCTTGTTGCCACAGTCTCCAACTTACCTTATCTACAACACAATATTCAATATCCAATTGATACAATCATACAAACATTGGATTGGGTTAACTTGATTTCTTATGATTTTTATACCCCTACTTCTTCAAGGAATTGTACTGGACCATCCTCAGCCTTCTACAATCCAAGATCCAACTCTTTGTCGGCAAATTCTGGTATGAACTAAGTGCATGTTTGAAAATAATTTTAGAAATATTGATTTTAGTGTAATTGATTTTTCTATAATTGATTTATTAAAAGTGATTTTCATATAATCATTTTCATATTTTGTTCTATACTTTTAATAGTAATTTTGGTTTTGAAAAAATTCATTTTTATGGGAATTTATGAATTTTGACAAAAGATTATGGTTTTTACTCGTTATTAAAATATATTATTTTTAAAAGTGAATAATAGTCATTTTAAATTACAATCACTTGACAAAAATCACTTGGAAATCACCTCCCAAGTGATTGAAAGGGAATCATTTCAAATGATTTTGGATTTTTCCAAAATCACTTGCTTAATATTGCCAAAATCACTTGCTTAATATTGCCAAACATCACACTTTGGGTATGAAAGTGATTTTAGACATGTCAAAAGTGATTTTAGTCACTCCAAAATCACTCCCAAACATGCCCTAACTATGGTTTTAGTTATATATGTTTGAGTGGTTTTTCAATTTACATTTTTATAAGTTGCAATTTTAATAGTGTGTTATGTTTTAATATATTTTAATTATATCATTTTGTAGAATATATGGTCAATGTTAAAAATGGTTAAGGACAAACTTGCATTACATAATGACAAAATTTTGGAGGAGGAATGCGTTTAGAAGGAAAAGTTTTTAATTCCTCTCTCAAATTTCTAAGGATTTGACTAGTAATTTTTTGACTTTTTTTTTTACCATTAGATGACATAATTCTTTCTCTAAAGTTTTGTCAACTCATTCACTAAGTATCATACCATTTAAGCTTATCATCAATCATTATTGGTCGTTCACCTATAAAAAAGGTATAATAAAAAAAATGTTAAAGATGGTGGGGATAAAATTGAAAATTTTTAAACAATAGTTAGGCACTTAAATCGTAAGAACACTCAAACCGTCAAGGTTAGGGCATGTGTTTAGCCTCGATTAAATAATACTACGTTACATTCAATCTTTCATTTCATTTCATTTGCCTTAATTAATGCTTAATATTGATTACTTGTGTTGAATTAATTGAAAAAGGTATTGAGGCGTGGATTAAATGTTTTCCACATTTGCCTTCTGAAAAGATTGTATTTGGAATTCCTTTTCATGGTTGGGCATGGAAACTTACTGATCCTCGACAACACGAAGTATTTTCCAAAGCAGATGGGCCAGCCACAAGAGAGAGTATTTCTTTAGATGGTCGAATCGACTACTCTAATATTATAAAGTTTATAGAAGAGAATAAAGCAGAAAATGTGAAAAAAGACGAGATATACGTAATTGGGCACACATTTCATGGTTCAACATGGATTGCATATGAAAATGAAGAGACCATACCAGAGAAGATTAGATTTGCAAAGTCGCACCATGTGCATGGTTACTTTGCTTGGAACATTGCCGCTGATGATAATGTTAACACTCTTGCTAATGCAGCTCAAAGTAGTTGGTGA

mRNA sequence

ATGTCCTCAAGGCATCATGTCCAAGCTGGTTACTGGTTCACCAGCACTGACTTCCGACTTCCCTCAGCAAATATTGATGCTAATTATTTTACTCATCTCTTTTGTGGTTATGTTGGTGTCGACGCCCAAACTTATGAAGTCGCCGTAAGTCCTTTAGAAGAGGATTTGATTTATCAATTTACTCCAACCGTCAGACTAAGCAATCATAGAGTGAAAACCCTATTGTCCATCGGAGGAGATAATGATGTGTTTGCTAAAATGGCTAGCGAGCCTACAAATCGTGATGCTTTTATTGAATCCTCCATTTCAATAGCAAGAGCAGGTGAATTTGATGGCCTCGATCTTCAATGGTTTTTTCCATCTTCTCAAGAGGAAATGGATGATTTTGAGAATCTCCTCTGTGAATGGCATAAAGCTGCTATCAAAGAAGCTAAAGAATATCATAGAACAAAATTGTTGCTTGTTGCCACAGTCTCCAACTTACCTTATCTACAACACAATATTCAATATCCAATTGATACAATCATACAAACATTGGATTGGGTTAACTTGATTTCTTATGATTTTTATACCCCTACTTCTTCAAGGAATTGTACTGGACCATCCTCAGCCTTCTACAATCCAAGATCCAACTCTTTGTCGGCAAATTCTGGTATTGAGGCGTGGATTAAATGTTTTCCACATTTGCCTTCTGAAAAGATTGTATTTGGAATTCCTTTTCATGGTTGGGCATGGAAACTTACTGATCCTCGACAACACGAAGTATTTTCCAAAGCAGATGGGCCAGCCACAAGAGAGAGTATTTCTTTAGATGGTCGAATCGACTACTCTAATATTATAAAGTTTATAGAAGAGAATAAAGCAGAAAATGTGAAAAAAGACGAGATATACGTAATTGGGCACACATTTCATGGTTCAACATGGATTGCATATGAAAATGAAGAGACCATACCAGAGAAGATTAGATTTGCAAAGTCGCACCATGTGCATGGTTACTTTGCTTGGAACATTGCCGCTGATGATAATGTTAACACTCTTGCTAATGCAGCTCAAAGTAGTTGGTGA

Coding sequence (CDS)

ATGTCCTCAAGGCATCATGTCCAAGCTGGTTACTGGTTCACCAGCACTGACTTCCGACTTCCCTCAGCAAATATTGATGCTAATTATTTTACTCATCTCTTTTGTGGTTATGTTGGTGTCGACGCCCAAACTTATGAAGTCGCCGTAAGTCCTTTAGAAGAGGATTTGATTTATCAATTTACTCCAACCGTCAGACTAAGCAATCATAGAGTGAAAACCCTATTGTCCATCGGAGGAGATAATGATGTGTTTGCTAAAATGGCTAGCGAGCCTACAAATCGTGATGCTTTTATTGAATCCTCCATTTCAATAGCAAGAGCAGGTGAATTTGATGGCCTCGATCTTCAATGGTTTTTTCCATCTTCTCAAGAGGAAATGGATGATTTTGAGAATCTCCTCTGTGAATGGCATAAAGCTGCTATCAAAGAAGCTAAAGAATATCATAGAACAAAATTGTTGCTTGTTGCCACAGTCTCCAACTTACCTTATCTACAACACAATATTCAATATCCAATTGATACAATCATACAAACATTGGATTGGGTTAACTTGATTTCTTATGATTTTTATACCCCTACTTCTTCAAGGAATTGTACTGGACCATCCTCAGCCTTCTACAATCCAAGATCCAACTCTTTGTCGGCAAATTCTGGTATTGAGGCGTGGATTAAATGTTTTCCACATTTGCCTTCTGAAAAGATTGTATTTGGAATTCCTTTTCATGGTTGGGCATGGAAACTTACTGATCCTCGACAACACGAAGTATTTTCCAAAGCAGATGGGCCAGCCACAAGAGAGAGTATTTCTTTAGATGGTCGAATCGACTACTCTAATATTATAAAGTTTATAGAAGAGAATAAAGCAGAAAATGTGAAAAAAGACGAGATATACGTAATTGGGCACACATTTCATGGTTCAACATGGATTGCATATGAAAATGAAGAGACCATACCAGAGAAGATTAGATTTGCAAAGTCGCACCATGTGCATGGTTACTTTGCTTGGAACATTGCCGCTGATGATAATGTTAACACTCTTGCTAATGCAGCTCAAAGTAGTTGGTGA

Protein sequence

MSSRHHVQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRLSNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLISYDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW
Homology
BLAST of Tan0020355 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-72
Identity = 149/353 (42.21%), Postives = 217/353 (61.47%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V+A YWF +++F  P  +ID++ FTHLFC +  +++QT +V VS   +     FT TV+ 
Sbjct: 28  VKASYWFPASEF--PVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 67  SNHRVKTLLSIGG---DNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQ 126
            N  VKTLLSIGG   D   +A MAS PT+R +FI+SSI +AR+  F GLDL W +PSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 127 EEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATV--SNLPYLQHNIQYPIDTIIQTLDW 186
            EM +F  LL EW  A + EA    + +LLL A V  SN  Y   ++ YP+  +  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 187 VNLISYDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFH 246
           VNL++YDFY P  SR  TGP +A ++P +   S ++G  +WI+    LP++K V G P++
Sbjct: 208 VNLMAYDFYGPGWSR-VTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYY 267

Query: 247 GWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGH 306
           G+AW+LT+   H  ++    P T  +IS DG I Y  I KFI +N A  V    + V  +
Sbjct: 268 GYAWRLTNANSHSYYA----PTTGAAISPDGSIGYGQIRKFIVDNGATTVYNSTV-VGDY 327

Query: 307 TFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
            + G+ WI Y++ ++I  K+R+AK   + GYF+W++ ADDN + L+ AA  +W
Sbjct: 328 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of Tan0020355 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 212.6 bits (540), Expect = 7.2e-54
Identity = 130/365 (35.62%), Postives = 213/365 (58.36%), Query Frame = 0

Query: 3   SRHHVQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPL------EEDL 62
           S+  V+A YW   +DF   +++ID NYFTH++  +V    +++ + ++        + D 
Sbjct: 51  SKGGVRAAYWPAWSDF--STSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDG 110

Query: 63  IYQFTPTVRLSNHRVKTLLSIGGDND---VFAKMASEPTNRDAFIESSISIARAGEFDGL 122
           I+   P       RV TLLSIGG  +   +F++MAS   NR +FI S+I +AR  EF+GL
Sbjct: 111 IHNIRP-------RVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGL 170

Query: 123 DLQWFFPSSQEEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATV---SNLPYLQHNIQ- 182
           DL W +P  +++M +   LL EW+KA + EA    +++LLL + V   S +  + +  + 
Sbjct: 171 DLDWEWPGDEKDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRS 230

Query: 183 YPIDTIIQTLDWVNLISYDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHL 242
           YP+  I + LDW + + +D+    +  N TG ++A Y+P SN +S   GI +WI     +
Sbjct: 231 YPVRAIRKYLDWASPMCFDYNGAWA--NETGFNAALYDPNSN-ISTKYGIGSWIG--SGV 290

Query: 243 PSEKIVFGIPFHGWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAE 302
           P+EK+V G+P +G AW+L DP  H V +KA GPA    +  DG +DY  I+ F ++  A+
Sbjct: 291 PAEKLVMGLPLYGRAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVFNKDTGAK 350

Query: 303 NVKKDEIYVIGHTFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANA 355
            V  DE+ V  +++ G+TWI Y++  +I +K++FA+S  + GYF W I  D +  T++  
Sbjct: 351 -VVYDEVAVSFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDW-TISKQ 395

BLAST of Tan0020355 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.8e-49
Identity = 122/362 (33.70%), Postives = 203/362 (56.08%), Query Frame = 0

Query: 5   HHVQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTV 64
           H V++ YW    DF  PS  ID NYFTH+   ++  +  ++++ ++         F   +
Sbjct: 38  HGVRSAYWPAGDDFS-PSL-IDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKAL 97

Query: 65  RLSNHRVKTLLSIGG---DNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPS 124
           R  +  VKTLLSIGG   ++ +F+++AS   NR+ FI S+I +AR   FDG+DL W FP 
Sbjct: 98  RHRSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPE 157

Query: 125 SQEEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQ---------YPI 184
           +Q++M +   L  EW+ A   EAK   + +LLL + V    Y    ++         YP 
Sbjct: 158 TQQDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAV----YYNSTVRLIGKHGPRSYPT 217

Query: 185 DTIIQTLDWVNLISYDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSE 244
             I + LDW + + +D++      N T  ++A Y+ +S  +S N G+ +WIK    +  E
Sbjct: 218 QAINKYLDWASPMCFDYHGTWD--NNTDFNAALYDSKS-EISTNFGLHSWIK--SGVRPE 277

Query: 245 KIVFGIPFHGWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVK 304
           K+V G+  +G AW+L DP  + V ++A GPAT      DG ++Y+ I+KF +++ A NV 
Sbjct: 278 KLVMGLALYGRAWELKDPNVNGVGAEAVGPAT----DTDGSMNYNEILKFNKQSGA-NVV 337

Query: 305 KDEIYVIGHTFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQS 355
            D++ +  +++ G+TWI Y++  +I  K+RFAKS  + GYF W +  D +  +++  A +
Sbjct: 338 YDKVAISFYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKDW-SISKQASN 382

BLAST of Tan0020355 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 4.1e-49
Identity = 128/365 (35.07%), Postives = 194/365 (53.15%), Query Frame = 0

Query: 2   SSRHHVQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEED------ 61
           SS   V+  YW  +  F  P ++ID + FTH+F  +V  +  TY++     EED      
Sbjct: 32  SSTTRVKGIYWIENPLF--PPSSIDTSLFTHIFYAFVSPNKFTYKLEEE--EEDSTTVAT 91

Query: 62  LIYQFTPTVRLSNHRVKTLLSIGG---DNDVFAKMASEPTNRDAFIESSISIARAGEFDG 121
            +  FT T +     + TLLSIGG   ++ +FA +AS+PT R  FI S+I +AR   FDG
Sbjct: 92  SLTTFTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDG 151

Query: 122 LDLQWFFPSSQEEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATV---SNLPYLQHNIQ 181
           +D  W FP++ +EM+D   LL +W +A   E     R  LLL A V    N         
Sbjct: 152 IDFDWEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRM 211

Query: 182 YPIDTIIQTLDWVNLISYDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHL 241
           YP+D+I + LDWVN++SYD     S  N TG  S  ++ +SN +S  SG+ +WI+    +
Sbjct: 212 YPVDSINKNLDWVNVMSYDL--RGSGSNVTGAPSGMFDSKSN-VSVVSGLFSWIR--GGV 271

Query: 242 PSEKIVFGIPFHGWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAE 301
             EKIV G+P +G +WKL DP  H + +   GP       +DG + Y  ++ F ++  A+
Sbjct: 272 APEKIVMGMPLYGKSWKLQDPNVHGIGAPNVGPGP----GVDGGMAYFQVVDFNKQMGAK 331

Query: 302 NVKKDEIYVIGHTFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANA 355
            V   E   + +++ GSTWI Y++  T+  K+ FA++  + GYF W  A  D  +   + 
Sbjct: 332 VVYDKETGSV-YSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFW-AAGYDTSDWKVST 381

BLAST of Tan0020355 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.9e-48
Identity = 120/359 (33.43%), Postives = 186/359 (51.81%), Query Frame = 0

Query: 2   SSRHHVQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFT 61
           SS   V+  YW  +  F  P   +D + FTH+F  ++  +  TY++ +S  +   +  FT
Sbjct: 30  SSITRVKGIYWLENPFF--PPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFT 89

Query: 62  PTVRLSNHRVKTLLSIGG---DNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWF 121
            T +  +    TL SIGG   ++ + A +AS+P    AFI S+I +AR   FDG+DL W 
Sbjct: 90  KTFKTKSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWE 149

Query: 122 FPSSQEEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATV---SNLPYLQHNIQYPIDTI 181
           FP + +EM+D   +L +W KA   E     R  LLL A V    N         YP+++I
Sbjct: 150 FPKNTKEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSI 209

Query: 182 IQTLDWVNLISYDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIV 241
            + LDWVN++SY+   P S  N TG  S  ++P+SN +S  SG+ +WI     +  EK+V
Sbjct: 210 NENLDWVNVMSYELRGPRS--NKTGAPSGTFDPKSN-VSVVSGLLSWI--HSGVVPEKLV 269

Query: 242 FGIPFHGWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDE 301
            G+P +G +WKL DP  H +      P+      ++G + Y  ++ F    K+  V+ D 
Sbjct: 270 MGMPLYGKSWKLRDPNVHGI----GAPSVGSGPGVNGLMAYFQVLDF-NRQKSAKVEYDV 329

Query: 302 IYVIGHTFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
                +++ GSTWI Y+N  T+  K+ FA++  + GYF W    D     +A  A  +W
Sbjct: 330 DTASVYSYSGSTWIGYDNPFTVSIKVGFAQALKLRGYFFWVAGLDTLDWKIATQASKAW 376

BLAST of Tan0020355 vs. NCBI nr
Match: XP_022143191.1 (chitotriosidase-1-like [Momordica charantia])

HSP 1 Score: 503.4 bits (1295), Expect = 1.5e-138
Identity = 244/349 (69.91%), Postives = 283/349 (81.09%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V+A YWF ST+  LPSANIDAN+FTHLFCGY  V+ QTYEV +   E  +I  FT TV L
Sbjct: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI GD  V AKMASEP NR+ FI+SSI  A+ G FDGLDLQW +PSSQEEM
Sbjct: 66  SNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQEEM 125

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
           ++FE+LL EWH A +++A+ YHR +L+LVA VSNLP + HNIQYPIDTIIQTLDWVNL S
Sbjct: 126 ENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNLFS 185

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTPTSS  CTGPSSA YNP+++SLS NSGIEAWIKC+P+LPS++IVFGIPFHGWAWK
Sbjct: 186 YDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWAWK 245

Query: 247 LTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHGS 306
           L D  QH+VFS+ADG A    IS DG+I YSNI KFIE NKA+N+K D  YVIG+T+  +
Sbjct: 246 LADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFDT 305

Query: 307 TWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQ-SSW 355
           TWIAYENE TIPEK+R AKS HV GYFA NIAADD+VNTLANAA+ +SW
Sbjct: 306 TWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVNTLANAAKANSW 354

BLAST of Tan0020355 vs. NCBI nr
Match: XP_022159350.1 (chitinase-3-like protein 2 [Momordica charantia])

HSP 1 Score: 441.0 bits (1133), Expect = 9.4e-120
Identity = 214/349 (61.32%), Postives = 268/349 (76.79%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V  GYW +ST+ +LP +NIDA  FTHLFCG+  +D  +YEV V   +  LI QF+ TVR+
Sbjct: 11  VNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQFSSTVRM 70

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI GD DVFAKMASEPT RD FI+SSI +A  G+F+GLDLQW +PSS+EEM
Sbjct: 71  SNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSEEEM 130

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
            +FE LL  W +AA+ +AK YHR + +LVA+VSNLPY++ +I YP++ I Q LDWVNLIS
Sbjct: 131 QNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLDWVNLIS 190

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTP SS + TGPSSA +N  + SLSAN GI+AWI+   +LP ++IVFGIPFHGWAWK
Sbjct: 191 YDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPFHGWAWK 250

Query: 247 LTDPRQHEVFSKADGPATRES-ISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHG 306
           L +P +H+VFSKA GPA  +  IS +G+ID SN+ +FI++N A+ VK D  Y I + + G
Sbjct: 251 LANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRYSISYAYAG 310

Query: 307 STWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
           +TWIAYE+E TIP+KI  AKS  V GYFAWNIAADD VNTLANAAQ++W
Sbjct: 311 TTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNW 359

BLAST of Tan0020355 vs. NCBI nr
Match: XP_023545402.1 (acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 436.4 bits (1121), Expect = 2.3e-118
Identity = 212/348 (60.92%), Postives = 264/348 (75.86%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V AGYW  STD RLP+ANIDA  FTHLF GY  +++ TY+V + P +  L+ QF+PTVRL
Sbjct: 2   VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSPTVRL 61

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI  D  VFA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQEEM
Sbjct: 62  SNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQEEM 121

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
            +F+ L+  WH+A + +A      +LLLVATVSNLPYLQ NIQYP D I Q LDWVNL+S
Sbjct: 122 GNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 181

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTP S+R  TGPSSAF+NP++N+LSAN GI +W +   +LP +KIVFGIPFHGWAWK
Sbjct: 182 YDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 241

Query: 247 LTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHGS 306
           L +  QH +F+KADG AT E I   G+I YS++ KFIE+N+A    KD+ Y++ +T+  +
Sbjct: 242 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYTYFET 301

Query: 307 TWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
           TWI YE++ TI +KI  AK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 302 TWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 349

BLAST of Tan0020355 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 436.4 bits (1121), Expect = 2.3e-118
Identity = 212/348 (60.92%), Postives = 264/348 (75.86%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V AGYW  STD RLP+ANIDA  FTHLF GY  +++ TY+V + P +  L+ QF+PTVRL
Sbjct: 17  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSPTVRL 76

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI  D  VFA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQEEM
Sbjct: 77  SNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQEEM 136

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
            +F+ L+  WH+A + +A      +LLLVATVSNLPYLQ NIQYP D I Q LDWVNL+S
Sbjct: 137 GNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 196

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTP S+R  TGPSSAF+NP++N+LSAN GI +W +   +LP +KIVFGIPFHGWAWK
Sbjct: 197 YDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 256

Query: 247 LTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHGS 306
           L +  QH +F+KADG AT E I   G+I YS++ KFIE+N+A    KD+ Y++ +T+  +
Sbjct: 257 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYTYFET 316

Query: 307 TWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
           TWI YE++ TI +KI  AK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 317 TWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 364

BLAST of Tan0020355 vs. NCBI nr
Match: KAG7030350.1 (Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 434.9 bits (1117), Expect = 6.8e-118
Identity = 210/348 (60.34%), Postives = 264/348 (75.86%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V AGYW  STD RLP+ANIDA  FTHLF GY  +++ TY+V + P    LI QF+ TV+L
Sbjct: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSHLILIDQFSSTVKL 70

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI  D  +FA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQ++M
Sbjct: 71  SNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
           D+F+ L+  WH+A + +A      +LLLVATVSNLPYLQ NIQYP D I Q LDWVNL+S
Sbjct: 131 DNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 190

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTP SSR  TGPSSAF+NP++N+LSAN GI +W++   +LP +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWVQSLHNLPHKKIVFGIPFHGWAWK 250

Query: 247 LTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHGS 306
           L +  QH +F+KADG AT E I   G+I YS++ KFIE+N+A    KD+ Y++ + ++ +
Sbjct: 251 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
           TWI YE+E TI +KI  AK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0020355 vs. ExPASy TrEMBL
Match: A0A6J1CPI6 (chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 7.5e-139
Identity = 244/349 (69.91%), Postives = 283/349 (81.09%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V+A YWF ST+  LPSANIDAN+FTHLFCGY  V+ QTYEV +   E  +I  FT TV L
Sbjct: 6   VRAAYWFASTNAELPSANIDANFFTHLFCGYAIVNPQTYEVTIDSSEATIINYFTLTVSL 65

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI GD  V AKMASEP NR+ FI+SSI  A+ G FDGLDLQW +PSSQEEM
Sbjct: 66  SNPTVKTLLSIKGDGSVLAKMASEPKNRETFIKSSIKEAKEGTFDGLDLQWLYPSSQEEM 125

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
           ++FE+LL EWH A +++A+ YHR +L+LVA VSNLP + HNIQYPIDTIIQTLDWVNL S
Sbjct: 126 ENFESLLIEWHSAVVEDARNYHRQQLILVAAVSNLPIVHHNIQYPIDTIIQTLDWVNLFS 185

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTPTSS  CTGPSSA YNP+++SLS NSGIEAWIKC+P+LPS++IVFGIPFHGWAWK
Sbjct: 186 YDFYTPTSSVKCTGPSSALYNPKTDSLSVNSGIEAWIKCYPNLPSQQIVFGIPFHGWAWK 245

Query: 247 LTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHGS 306
           L D  QH+VFS+ADG A    IS DG+I YSNI KFIE NKA+N+K D  YVIG+T+  +
Sbjct: 246 LADRLQHDVFSEADGAAIGHDISSDGQIYYSNIKKFIESNKAKNLKNDGKYVIGYTYFDT 305

Query: 307 TWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQ-SSW 355
           TWIAYENE TIPEK+R AKS HV GYFA NIAADD+VNTLANAA+ +SW
Sbjct: 306 TWIAYENEVTIPEKLRLAKSLHVLGYFASNIAADDDVNTLANAAKANSW 354

BLAST of Tan0020355 vs. ExPASy TrEMBL
Match: A0A6J1DYG4 (chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 4.6e-120
Identity = 214/349 (61.32%), Postives = 268/349 (76.79%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V  GYW +ST+ +LP +NIDA  FTHLFCG+  +D  +YEV V   +  LI QF+ TVR+
Sbjct: 11  VNGGYWLSSTNQQLPPSNIDAKLFTHLFCGFAEIDRNSYEVTVPASQATLINQFSSTVRM 70

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI GD DVFAKMASEPT RD FI+SSI +A  G+F+GLDLQW +PSS+EEM
Sbjct: 71  SNPDVKTLLSIRGDRDVFAKMASEPTYRDGFIQSSIEVAAKGKFNGLDLQWLYPSSEEEM 130

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
            +FE LL  W +AA+ +AK YHR + +LVA+VSNLPY++ +I YP++ I Q LDWVNLIS
Sbjct: 131 QNFEILLGAWRQAAVDDAKTYHRPQWILVASVSNLPYVESSIVYPVEEIKQNLDWVNLIS 190

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTP SS + TGPSSA +N  + SLSAN GI+AWI+   +LP ++IVFGIPFHGWAWK
Sbjct: 191 YDFYTPASSPSATGPSSALHNRMARSLSANFGIDAWIQSLANLPRKQIVFGIPFHGWAWK 250

Query: 247 LTDPRQHEVFSKADGPATRES-ISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHG 306
           L +P +H+VFSKA GPA  +  IS +G+ID SN+ +FI++N A+ VK D  Y I + + G
Sbjct: 251 LANPDKHDVFSKALGPAKGDQYISQNGQIDXSNVKRFIKQNHAQYVKDDSRYSISYAYAG 310

Query: 307 STWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
           +TWIAYE+E TIP+KI  AKS  V GYFAWNIAADD VNTLANAAQ++W
Sbjct: 311 TTWIAYESEYTIPDKISSAKSSKVLGYFAWNIAADDEVNTLANAAQTNW 359

BLAST of Tan0020355 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 3.3e-118
Identity = 212/348 (60.92%), Postives = 264/348 (75.86%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V AGYW  STD RLP+ANIDA  FTHLF GY  +++ TY+V V P +  LI QF+ TV+L
Sbjct: 11  VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSSTVKL 70

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI  D  VFA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQ++M
Sbjct: 71  SNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
           D+F+ L+  WH+A + +A      +LLLVATVSNLPYLQ NIQYP D I Q LDWVNL+S
Sbjct: 131 DNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 190

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTP SSR  TGPSSAF+NP++N+LSAN GI +W +   +LP +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 250

Query: 247 LTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHGS 306
           L +  QH +F+KADG AT E I   G+I YS++ KFIE+N+A    KD+ Y++ + ++ +
Sbjct: 251 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
           TWI YE+E TI +KI  AK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0020355 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 2.0e-115
Identity = 206/348 (59.20%), Postives = 262/348 (75.29%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V AGYW  STD  LP+ANIDA  FTHLF GY  +++ TY+V V P +  LI QF+ TV+L
Sbjct: 11  VNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSSTVKL 70

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI GD  VFA MA +PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQ++M
Sbjct: 71  SNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
            +F+ L+  WH+A + +A      +LLLVATVSNLPYLQ+NIQYP D I Q LDWVNL+S
Sbjct: 131 ANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLDWVNLLS 190

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTP S R+ TGPSSAF+NP++N+LSAN GI +W +   +L  +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPFHGWAWK 250

Query: 247 LTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHGS 306
           L +  QH +F+KADG A+ E I   G+I YS++ KFIE+N+A    KD+ Y++ + ++ +
Sbjct: 251 LANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
           TWI +E+E T+ +KI  AK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0020355 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 2.6e-115
Identity = 206/348 (59.20%), Postives = 262/348 (75.29%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V AGYW  STD  LP+ANIDA  FTHLF GY  +++ TY+V V P +  LI QF+ TV+L
Sbjct: 11  VNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSSTVKL 70

Query: 67  SNHRVKTLLSIGGDNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQEEM 126
           SN  VKTLLSI GD  VFA MA++PTNRDAFI+SSI+ AR G FDGLDLQW +PSSQ++M
Sbjct: 71  SNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQDQM 130

Query: 127 DDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVNLIS 186
            +F+ L+  WH+A + +A      +LLLVATVSNLPYLQ+NIQYP D I Q LDWVNL+S
Sbjct: 131 ANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLDWVNLLS 190

Query: 187 YDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFHGWAWK 246
           YDFYTP S R  TGPSSAF+NP++N+LSAN GI +W +   +L  +KIVFGIPFHGWAWK
Sbjct: 191 YDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPFHGWAWK 250

Query: 247 LTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHTFHGS 306
           L +  QH +F+KADG A+ E I   G+I YS++ KFIE+N+A    KD+ Y++ + ++ +
Sbjct: 251 LANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYAYYET 310

Query: 307 TWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
           TWI +E+E T+ +KI  AK+  V GYFA+NIAADD  NTLANAA+S+W
Sbjct: 311 TWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Tan0020355 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 275.0 bits (702), Expect = 8.3e-74
Identity = 149/353 (42.21%), Postives = 217/353 (61.47%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V+A YWF +++F  P  +ID++ FTHLFC +  +++QT +V VS   +     FT TV+ 
Sbjct: 28  VKASYWFPASEF--PVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 67  SNHRVKTLLSIGG---DNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQ 126
            N  VKTLLSIGG   D   +A MAS PT+R +FI+SSI +AR+  F GLDL W +PSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 127 EEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATV--SNLPYLQHNIQYPIDTIIQTLDW 186
            EM +F  LL EW  A + EA    + +LLL A V  SN  Y   ++ YP+  +  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 187 VNLISYDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGIPFH 246
           VNL++YDFY P  SR  TGP +A ++P +   S ++G  +WI+    LP++K V G P++
Sbjct: 208 VNLMAYDFYGPGWSR-VTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPYY 267

Query: 247 GWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGH 306
           G+AW+LT+   H  ++    P T  +IS DG I Y  I KFI +N A  V    + V  +
Sbjct: 268 GYAWRLTNANSHSYYA----PTTGAAISPDGSIGYGQIRKFIVDNGATTVYNSTV-VGDY 327

Query: 307 TFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSSW 355
            + G+ WI Y++ ++I  K+R+AK   + GYF+W++ ADDN + L+ AA  +W
Sbjct: 328 CYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of Tan0020355 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 267.3 bits (682), Expect = 1.7e-71
Identity = 146/344 (42.44%), Postives = 206/344 (59.88%), Query Frame = 0

Query: 2   SSRHHVQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFT 61
           S++  V+A YWF  ++   P A ID++ FTHLFC +  ++  TY+V VS   +     FT
Sbjct: 21  SAQTVVKATYWFAESE--SPLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFT 80

Query: 62  PTVRLSNHRVKTLLSIGGD---NDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWF 121
            TVR  N  VKTLLSIGGD   N  FA MAS PT+R  FI SSI +AR+  F GLDL W 
Sbjct: 81  QTVRRRNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWK 140

Query: 122 FPSSQEEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQT 181
           +PS   EMD+F  LL EW  A   EA+   + +LLL A V    Y  +++ +P++ +  +
Sbjct: 141 YPSITTEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADS 200

Query: 182 LDWVNLISYDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKIVFGI 241
           LDWVNL++YDFY   SSR    P +  Y+P +   S ++G+ AW +    LP++K V G 
Sbjct: 201 LDWVNLVAYDFYESGSSRVTCSP-APLYDPITTGPSGDAGVRAWTQA--GLPAKKAVLGF 260

Query: 242 PFHGWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYV 301
           P +G+AW LTD + H  ++ + GPA    IS DG I Y  I +FI +NKA  V    + V
Sbjct: 261 PLYGYAWCLTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKATMVYNSNL-V 320

Query: 302 IGHTFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDN 343
             + +   TWI Y++ ++I  K+++AK   + GYF+W+I ADDN
Sbjct: 321 QNYCYAKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDN 353

BLAST of Tan0020355 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 263.1 bits (671), Expect = 3.3e-70
Identity = 145/348 (41.67%), Postives = 210/348 (60.34%), Query Frame = 0

Query: 1   MSSRHHVQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQF 60
           MS    V+A YWF +TDF  P+ +ID++ FTHLFC +  ++A++YE+ ++   +   + F
Sbjct: 1   MSESDVVKASYWFPATDF--PATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAF 60

Query: 61  TPTVRLSNHRVKTLLSIGG---DNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQW 120
           T TV+  N  VKTLLSIGG   D D FA MAS P +R +FI+S+I++AR+  F GLDL W
Sbjct: 61  TETVQQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDW 120

Query: 121 FFPSSQEEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQ---YPIDT 180
            +P ++EEM DF  LL EW  A   E+     T L+L A V    Y   N Q   YP+  
Sbjct: 121 EYPRNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLA 180

Query: 181 IIQTLDWVNLISYDFYTPTSSRNCTGPSSAFYNPRSNSLSANSGIEAWIKCFPHLPSEKI 240
           I  +LDW+NL++YDFY P  S   TGP ++ Y P ++  S +SG+  W +    LP++K 
Sbjct: 181 ISNSLDWINLMAYDFYGPGWS-TVTGPPASLYLP-TDGRSGDSGVRDWTEA--GLPAKKA 240

Query: 241 VFGIPFHGWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKD 300
           V G P++GWAW L DP  +   +   GPA    IS DG I Y  +  +I +N A  V  D
Sbjct: 241 VLGFPYYGWAWTLADPDVNGYDANTTGPA----ISDDGEISYRQLQTWIVDNGATKV-HD 300

Query: 301 EIYVIGHTFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDN 343
           ++ V  + + G+TWI Y++E++I  K+ +AK   + GYF+W++  DDN
Sbjct: 301 DMMVGDYCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN 333

BLAST of Tan0020355 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 240.7 bits (613), Expect = 1.7e-63
Identity = 136/340 (40.00%), Postives = 196/340 (57.65%), Query Frame = 0

Query: 7   VQAGYWFTSTDFRLPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIYQFTPTVRL 66
           V+A YW    +   P+ NID+  FTHLFC +  VD+ T+EV +S      +  FT TV+ 
Sbjct: 14  VKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTVKD 73

Query: 67  SNHRVKTLLSIGG---DNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDLQWFFPSSQ 126
            N  V+TLLSIGG   D  V A MAS   NR AFI+SSI IAR  +F GLDL W +PS+ 
Sbjct: 74  KNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPSND 133

Query: 127 EEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTIIQTLDWVN 186
            EM +F  L+ EW  A ++E+   ++  LLL A V   P   +  +YP+  I   LD+VN
Sbjct: 134 VEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPD-YYGEEYPVQAIADNLDFVN 193

Query: 187 LISYDFYTPTSSRNCTGPSSAFYNPRSNS-LSANSGIEAWIKCFPHLPSEKIVFGIPFHG 246
           +++YDFY P  S   TGP +A ++P + +  S +SG+  W++    LP++K V G  + G
Sbjct: 194 IMAYDFYGPGWS-PVTGPPAALFDPSNPAGRSGDSGLSKWLEA--KLPAKKAVLGFSYCG 253

Query: 247 WAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKKDEIYVIGHT 306
           WAW L D   +   +  DG A    IS DG I Y+ I  +I +N A     D   +  + 
Sbjct: 254 WAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGAATF-HDPAVIGFYC 313

Query: 307 FHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDN 343
           + G+TWI Y++ ++I  K+R+AK   + GYF+W++ AD N
Sbjct: 314 YVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGADYN 344

BLAST of Tan0020355 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 240.4 bits (612), Expect = 2.3e-63
Identity = 144/361 (39.89%), Postives = 201/361 (55.68%), Query Frame = 0

Query: 7   VQAGYWFTSTDFR--------LPSANIDANYFTHLFCGYVGVDAQTYEVAVSPLEEDLIY 66
           V+A YWF     +         PS+ ID+  FTHLFC +  VD+ T+EV +S        
Sbjct: 14  VKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQFS 73

Query: 67  QFTPTVRLSNHRVKTLLSIGG---DNDVFAKMASEPTNRDAFIESSISIARAGEFDGLDL 126
            FT TV+  N  V+TLLSIGG   D  V A MAS   NR AFI+SSI IAR  +F GLDL
Sbjct: 74  SFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDL 133

Query: 127 QWFFPSSQEEMDDFENLLCEWHKAAIKEAKEYHRTKLLLVATVSNLPYLQHNIQYPIDTI 186
            W +PS+  EM +F  LL EW  A ++E+ + ++  LLL A V   P     ++YP+  I
Sbjct: 134 AWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQYD-GVEYPVKAI 193

Query: 187 IQTLDWVNLISYDFYTPTSSRNCTGPSSAFYNPRSN--SLSANSGIEAWIKCFPHLPSEK 246
              LD+VN+++YDFY P  S   TGP +A ++  SN    S NSG+  W+     LP +K
Sbjct: 194 ADNLDFVNIMAYDFYGPGWS-PVTGPPAALFHDPSNPAGRSGNSGLRKWLD-EAKLPPKK 253

Query: 247 IVFGIPFHGWAWKLTDPRQHEVFSKADGPATRESISLDGRIDYSNIIKFIEENKAENVKK 306
            V G P+ GWAW L D   +   +  DG A    IS DG I Y+ I  +I +N A     
Sbjct: 254 AVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNYIVDNGAATF-H 313

Query: 307 DEIYVIGHTFHGSTWIAYENEETIPEKIRFAKSHHVHGYFAWNIAADDNVNTLANAAQSS 355
           D   +  + + G+TWI Y++ ++I  K+++AK   + GYF+W++ AD N   L+ AA  +
Sbjct: 314 DPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNCG-LSRAASIA 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818621.2e-7242.21Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A1B1J8Z27.2e-5435.62Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
A0A072VEP01.8e-4933.70Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
U5N4E34.1e-4935.07Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
A0A072UR655.9e-4833.43Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022143191.11.5e-13869.91chitotriosidase-1-like [Momordica charantia][more]
XP_022159350.19.4e-12061.32chitinase-3-like protein 2 [Momordica charantia][more]
XP_023545402.12.3e-11860.92acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo][more]
XP_023521369.12.3e-11860.92acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo][more]
KAG7030350.16.8e-11860.34Chitotriosidase-1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1CPI67.5e-13969.91chitotriosidase-1-like OS=Momordica charantia OX=3673 GN=LOC111013120 PE=4 SV=1[more]
A0A6J1DYG44.6e-12061.32chitinase-3-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111025768 PE=4 S... [more]
A0A6J1G2P43.3e-11860.92acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE... [more]
A0A6J1KFT32.0e-11559.20chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1[more]
A0A6J1KB822.6e-11559.20chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G19810.18.3e-7442.21Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.11.7e-7142.44Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.13.3e-7041.67Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19750.11.7e-6340.00Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19760.12.3e-6339.89Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011583Chitinase IISMARTSM006362g34coord: 6..340
e-value: 6.0E-64
score: 228.4
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 9..340
e-value: 1.0E-59
score: 202.8
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 6..354
score: 48.052063
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 240..311
e-value: 2.6E-81
score: 275.5
IPR029070Chitinase insertion domain superfamilySUPERFAMILY54556Chitinase insertion domaincoord: 241..311
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 24..342
e-value: 2.6E-81
score: 275.5
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 3..344
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 3..344
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 9..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0020355.1Tan0020355.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0008061 chitin binding
molecular_function GO:0016787 hydrolase activity