Tan0020131 (gene) Snake gourd v1

Overview
NameTan0020131
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCytochrome p450
LocationLG06: 1603976 .. 1608414 (+)
RNA-Seq ExpressionTan0020131
SyntenyTan0020131
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAGTTTATGGGATGTTGTGGTAGTGACAACCATTTTTTTTACCACCATTATTCTGTATACGAACTGCTTTAGACTTCTCAGATCAAAATTCTGCAACCAACTTCCTTTAGGCTCTCTCGGTTGGCCTTTCATTGGTGAGACTATTGAGTTCATCTCCTGTGCTTATTCAGACCGCCCCGAAACCTTCATGGACAAGCGTCGTCAACTGTAAGTTAACTTACAAAAACCAACTTTATTACATCCTGGTTCCTTCAAATGCTGATCTGACTTGTGATTGCTTGTTAATGGAGTTGCTTATTTTTCTTATGAGCCAATGGTTCATTTTGGTTTGCAGGTATGGGAAGGTGTTCAAGTCACATATTTTTGGAAGTCCTACTATTGTTTCTACTGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCCAAGGCTTTCGTACCGTCTTACCCGAAATCTTTGACAGAATTGATGGGGAAATCTTCCATTTTGCTGATAAATGGGAGCTTGCAGAAGAGAATTCATGGTCTTGTGGGAGCTTTCTTCAAGTCTTCACAACTCAAAGCTCGAATAACCAAGGATATGGAATGTTATGTCAAACTATCTATGGAAAATTGGACTGAGAACAATCCTATTTACCTTCAAGATGAAGCCAAAAATGTCAGCTCTTTCTCTCTCTTTCCCTCTCCCTCTTTTGAAGCTTGAAATTAACCTGTGAATTCTCTTTTTCTTTTTCCCTTACATGAAGTTAACAAAAAGACAATAAATTAAAATGTCACACCATTACTGTGTTGATACTAGACAAAAACAGTGACTTTCTTTACCTGTTTTGAGTGAATCATTGATGCCCAAATAATCCATTTCTCTAGCTCCTACTTGGGTTTAAAGATGATTTCTATCAAAAGTAAAAGAACCCCACAGTTTTGCTATCATTCTGTTCAAATCATAGTGTCCAAGAATTTACCTTTTAATCATTTTAGCTATATATTTTCCTGTTCAAAAAGTTACTAAGTGAGCCCCTTTTGTCTCCAGATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTCTCAAGCAACAATTTCAAGAATTCATTGCGGGTCTTATGGCTTTGCCAGTGAACATTCCCGGAAGCAGGCTTTACCGATCGTTGCAGGCAAGTTGGTCGTAAAAGGTTTAATATCCCCCTTTACTAGTACTTTTTTACTGTATTCTAAAAAAATGATGGAGCTTCATTCTATTCTTGAATTTCAGGCCAAAAGAAATATGGTTAAACTAGTAAGGAAGATAATACAAGAAAGAAAAAGCAGTGGAATTTCCCCCATCCCAAAAGACGTCATGGAAGTCTTTCTCAAAGATGAAAGTGAACAACTAACAGATGATCTAATAGCCGAGAACATGATTGACATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTAAAATACCTCTCCGATTGTCCAGCAGCACTTCAACAGTTAACGGTATGCTATTTTTCTTACCAACTCTTGAACTTTTCAGTTCTTAGGTGAAAAATCTACCTTACTACTGAACCCAAAAGCTTAAATTGATGTATTATGATAAATTTAATCTTTAGCAGTATTTTAACACATCTCGTGGGCTTCAAAATTTGTAGAATCCTCAACAAGTGGTTAGTGATATTAATTACTCCGATACTATGTCAAATCACCAAATAACCCAAAAATTTAAGCAGATGAGTGAGGATAAATTTATATATATATTTTTTATCAATACTTTAACAATCACCATGGTGAACTTTGCTTCAATGAAATATGAATATGTACTCAGACTTTATCTAGTTGTACTTTCTATCACTTGGAAAGGAAATATCCTTATTTCCCTGTTCATTTTCACGATGTCTCTATCAGCCATGTTCCTTTTATGCCTGTCGCATGGTCCTCGGGTGCACTAGCTAGAACGATGACAAACCGACTTCCTAAAGTACTAACTGTTGAATGCATACAATTTTGTCTATTTGTAACACTAAGTAAAGAAGGTTAATCTATGCTCTTCCTTGGCTTCTTTGAATAGTTCTTAAACGATATGCCTGTTTGGACTCATATCAGGAGGAAAACTTGAAGATGAAAAGACTCAAAGAGCAGCTCGGAGAGTCGTTGCATTGGAGCGATTACTTATCGCTACCATTTACACAGAGTGTAATACATTAACCTTTTGAAAAAGTAGGAAGCTAGAATGCTTGGTGGTTTTATTGAATGTGTCTTCATTTGAATAGGTGATCACGGAGACGTTAAGGATGGGAAACATCATAATCGGTGTGATGCGGAAGGCGATGAAAGACATGAAGATAAAAGGATACCTAATACCGAAAGGTTGGTCGGTACTTACGTACTTTAGATCCGTTCATCTCGACGACAACCATTATGACTGGCCTTACAAATTCAATCCATGGAGGTGGCAAGTAAGAAGGAACATATTTCTCACTTTCATCTCTTTTATTCTATGTTTGTCTCTCCCTAAGGGTCTGTTTGAGGCGCAAGTTATAATAATCTATGGAATACTCTGCACCCCAAACACAGACTATTATAACCCACTAAACAACTCTGCATTTTAAATGATATTATATGATTCCACATACTATAATAACACACAGACTATTATAACACATATTATTATAACTCACTCAGTGCTCCAAACATCTCCTAATGAATTTGTGGTCCAATAGGATAGAGACTTGAGTAGTTGTTACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCCAGACTTGAAGCTTCCATCTTCCTTCATCACTTTGTCACTCAGTTCAGGTAGCTGCCAAAATCACTTTTCCATCTCAACTTTTTCACTGTTTTTCTTGTTTCAATTGGAATGAAAGCAAAGCCACTCTAAAAGTTTTGTGCCATATTGGCCCCTTAGGCATCTGATTTTGTATCTTATGTTTCCTTTGTAACTAGAATGTTGGACCTACTCCTAACATTATGCTCATATCCCACCTCACTAATCTGTAAAAATGATTTTGTCCATCCCAAGATTGACCCTTGGGTGAACAAAATTTTGATATCTTAGGTTAAATTTCTATTCTAGTACGGTTTCCCTACTTTCAAAAATTTGTTAAGTTACAATTTCAGTCTCTGAACTTTAGTTTGTGTTGAATAGATCTCTGAACTTTAAAAAGTATCTAATAAGTTCTTGAACTTTCCGTTTTATATAATAGGTCCATGAACTTTAAAATGTGTCTAATATGCACATAAACTCTCCATTTTGTGTCGAATAAATTTCTGAGTTCTCAATTTTGTGTCTAGTATACATTGACCTATTCAACGTTTTTACAAATTCACAAATCTATTGGAAACAAAATTGAGAATTTAATATCTCTATCAGACACCAAATTCAATTGAAGGTTTTATGTTTAATAGATTCGTTAATTCTTTGAAAGGTTTAGTATGACAAAGACCTATCAGACACGAAAGTCAAAGTTTAGAGAGCTATTAACCTATTAGACGCTAACTTGAAAGATCTGAGACTAAATTTGTATGTTAACCTTACAAATTTTCTCTTCGTATATACTATTTTTTTAGTATCGCTTTTATATGTACGTTCTCTCATTCATCTCTTAGTACACATGAACCTCCACGTGACACTCGTCTCTATGCACCTTCTTCGAGTACAAACACGAAGATGGTTTTCAAATAACTTTCTAATTAAACCATATGGTATTACAGATGGATTGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAAGAGGATGCCAGTTTGGGTGAAGATAAGAAGGGATCCATGATAATGACAAAGGGGTGCTGCCTTTTTGAGAAAGCCACAACTTCAATTAACTTCACCAATTACACAAACAACCAAACTTACCTAAAAAAAAGGGGGTTAAATTACAAGTATAGTCCCTGAATTTTGAGGTTTGTATCTATTTGGTCCCTGAACTTTCAAAAGTGTCAAATAGGTCCTTGAACTTTTAATTTTGTATCTATTTGGTCCCTAAACTTTGAAATATGTCTAATAAGTCCTCAAATTTTCAATTTTGTATCCAATAGGTCATTGAAATTTCAATTTTGTGTCTAATAAGTCCTTGACCTAGTTGACGTTTTTTAAAATTCATGAACTTACTAGGCACAAAATTGAAAGTTTGAGCTTCTATTGGACAAAAAAATAAATTTTATATATAATAGATCATTAATTTTAAAAAAATTGTGAATATGTTAAGCTATTAGAAGTTCAAGAATTAATTAGACATTTTTAAAGTCTAGGGACTTATTATATATAAAATTGAAAGATTGGGATCTATTTGACACTTTTTAAAGTTCAGGAACTAAATAGACACAAACATGCC

mRNA sequence

ATGGACAGTTTATGGGATGTTGTGGTAGTGACAACCATTTTTTTTACCACCATTATTCTGTATACGAACTGCTTTAGACTTCTCAGATCAAAATTCTGCAACCAACTTCCTTTAGGCTCTCTCGGTTGGCCTTTCATTGGTGAGACTATTGAGTTCATCTCCTGTGCTTATTCAGACCGCCCCGAAACCTTCATGGACAAGCGTCGTCAACTGTATGGGAAGGTGTTCAAGTCACATATTTTTGGAAGTCCTACTATTGTTTCTACTGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCCAAGGCTTTCGTACCGTCTTACCCGAAATCTTTGACAGAATTGATGGGGAAATCTTCCATTTTGCTGATAAATGGGAGCTTGCAGAAGAGAATTCATGGTCTTGTGGGAGCTTTCTTCAAGTCTTCACAACTCAAAGCTCGAATAACCAAGGATATGGAATGTTATGTCAAACTATCTATGGAAAATTGGACTGAGAACAATCCTATTTACCTTCAAGATGAAGCCAAAAATATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTCTCAAGCAACAATTTCAAGAATTCATTGCGGGTCTTATGGCTTTGCCAGTGAACATTCCCGGAAGCAGGCTTTACCGATCGTTGCAGGCCAAAAGAAATATGGTTAAACTAGTAAGGAAGATAATACAAGAAAGAAAAAGCAGTGGAATTTCCCCCATCCCAAAAGACGTCATGGAAGTCTTTCTCAAAGATGAAAGTGAACAACTAACAGATGATCTAATAGCCGAGAACATGATTGACATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTAAAATACCTCTCCGATTGTCCAGCAGCACTTCAACAGTTAACGGAGGAAAACTTGAAGATGAAAAGACTCAAAGAGCAGCTCGGAGAGTCGTTGCATTGGAGCGATTACTTATCGCTACCATTTACACAGAGTGTGATCACGGAGACGTTAAGGATGGGAAACATCATAATCGGTGTGATGCGGAAGGCGATGAAAGACATGAAGATAAAAGGATACCTAATACCGAAAGGTTGGTCGGTACTTACGTACTTTAGATCCGTTCATCTCGACGACAACCATTATGACTGGCCTTACAAATTCAATCCATGGAGGTGGCAAGATAGAGACTTGAGTAGTTGTTACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCCAGACTTGAAGCTTCCATCTTCCTTCATCACTTTGTCACTCAGTTCAGATGGATTGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAAGAGGATGCCAGTTTGGGTGAAGATAAGAAGGGATCCATGATAATGACAAAGGGGTGCTGCCTTTTTGAGAAAGCCACAACTTCAATTAACTTCACCAATTACACAAACAACCAAACTTACCTAAAAAAAAGGGGGTTAAATTACAAGTATAGTCCCTGAATTTTGAGGTTTGTATCTATTTGGTCCCTGAACTTTCAAAAGTGTCAAATAGGTCCTTGAACTTTTAATTTTGTATCTATTTGGTCCCTAAACTTTGAAATATGTCTAATAAGTCCTCAAATTTTCAATTTTGTATCCAATAGGTCATTGAAATTTCAATTTTGTGTCTAATAAGTCCTTGACCTAGTTGACGTTTTTTAAAATTCATGAACTTACTAGGCACAAAATTGAAAGTTTGAGCTTCTATTGGACAAAAAAATAAATTTTATATATAATAGATCATTAATTTTAAAAAAATTGTGAATATGTTAAGCTATTAGAAGTTCAAGAATTAATTAGACATTTTTAAAGTCTAGGGACTTATTATATATAAAATTGAAAGATTGGGATCTATTTGACACTTTTTAAAGTTCAGGAACTAAATAGACACAAACATGCC

Coding sequence (CDS)

ATGGACAGTTTATGGGATGTTGTGGTAGTGACAACCATTTTTTTTACCACCATTATTCTGTATACGAACTGCTTTAGACTTCTCAGATCAAAATTCTGCAACCAACTTCCTTTAGGCTCTCTCGGTTGGCCTTTCATTGGTGAGACTATTGAGTTCATCTCCTGTGCTTATTCAGACCGCCCCGAAACCTTCATGGACAAGCGTCGTCAACTGTATGGGAAGGTGTTCAAGTCACATATTTTTGGAAGTCCTACTATTGTTTCTACTGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCCAAGGCTTTCGTACCGTCTTACCCGAAATCTTTGACAGAATTGATGGGGAAATCTTCCATTTTGCTGATAAATGGGAGCTTGCAGAAGAGAATTCATGGTCTTGTGGGAGCTTTCTTCAAGTCTTCACAACTCAAAGCTCGAATAACCAAGGATATGGAATGTTATGTCAAACTATCTATGGAAAATTGGACTGAGAACAATCCTATTTACCTTCAAGATGAAGCCAAAAATATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTCTCAAGCAACAATTTCAAGAATTCATTGCGGGTCTTATGGCTTTGCCAGTGAACATTCCCGGAAGCAGGCTTTACCGATCGTTGCAGGCCAAAAGAAATATGGTTAAACTAGTAAGGAAGATAATACAAGAAAGAAAAAGCAGTGGAATTTCCCCCATCCCAAAAGACGTCATGGAAGTCTTTCTCAAAGATGAAAGTGAACAACTAACAGATGATCTAATAGCCGAGAACATGATTGACATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTAAAATACCTCTCCGATTGTCCAGCAGCACTTCAACAGTTAACGGAGGAAAACTTGAAGATGAAAAGACTCAAAGAGCAGCTCGGAGAGTCGTTGCATTGGAGCGATTACTTATCGCTACCATTTACACAGAGTGTGATCACGGAGACGTTAAGGATGGGAAACATCATAATCGGTGTGATGCGGAAGGCGATGAAAGACATGAAGATAAAAGGATACCTAATACCGAAAGGTTGGTCGGTACTTACGTACTTTAGATCCGTTCATCTCGACGACAACCATTATGACTGGCCTTACAAATTCAATCCATGGAGGTGGCAAGATAGAGACTTGAGTAGTTGTTACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCCAGACTTGAAGCTTCCATCTTCCTTCATCACTTTGTCACTCAGTTCAGATGGATTGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAAGAGGATGCCAGTTTGGGTGAAGATAAGAAGGGATCCATGA

Protein sequence

MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRKIIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKIRRDP
Homology
BLAST of Tan0020131 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 1.4e-174
Identity = 294/440 (66.82%), Postives = 363/440 (82.50%), Query Frame = 0

Query: 35  QLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVS 94
           + P GSLGWP IGETIEF+S AYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV+
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 95  KFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSQLKARITKDM 154
           + +LQSD+ AFVP YPK++ ELMGKSSILLINGSL +R HGLVG+F KS  LKA+I +DM
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 155 ECYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMA 214
             ++  SM+ W+E+ P+ LQD +K ++F+VL K LIS+  G+ +E LK++F+ FI+GLM+
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 215 LPVNIPGSRLYRSLQAKRNMVKLVRKIIQ-----ERKSSGISPIPKDVMEVFLKDESEQL 274
           LP+N PG++L+RSLQAK+NMVK V +II+      +       I KDV++V LKD SE L
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHL 289

Query: 275 TDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLH 334
           T +LIA NMIDMMIPG DSVP+L+TLAVK+LSD PAAL  LTEEN+K+K LKE  GE L+
Sbjct: 290 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 349

Query: 335 WSDYLSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDN 394
           W+DYLSLPFTQ VITETLRMGN+IIGVMRKAMKD++IKGY+IPKGW  L Y RSVHLD  
Sbjct: 350 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 409

Query: 395 HYDWPYKFNPWRWQDRDLSSCYFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEE 454
           +Y+ PYKFNPWRWQ+RD+++  F+PFGGGQRLCPGLDLARLE S+FLHH VT+FRWIAEE
Sbjct: 410 YYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEE 469

Query: 455 DRIINFPTVRMKKRMPVWVK 470
           D IINFPTV MK ++P+W+K
Sbjct: 470 DTIINFPTVHMKNKLPIWIK 489

BLAST of Tan0020131 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 4.8e-170
Identity = 280/442 (63.35%), Postives = 373/442 (84.39%), Query Frame = 0

Query: 35  QLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGK-VFKSHIFGSPTIVSTDAEV 94
           +LP GS GWP +GET+EF+SCAYS RPE F+DKRR+L+G  VF+SH+FGS T+V+ DAEV
Sbjct: 48  RLPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEV 107

Query: 95  SKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSQLKARITKD 154
           S+F+LQSDA+AFVP YP+SLTELMGKSSILLING+LQ+R+HGLVGAFFKSS LK+++T D
Sbjct: 108 SRFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTAD 167

Query: 155 MECYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPGQRMEFLKQQFQEFIAGLM 214
           M   +  ++ ++ +++ +++Q  AK++ F++LV+ LI L  G+ M+ LKQQFQEFI GLM
Sbjct: 168 MRRRLSPALSSFPDSSLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGLM 227

Query: 215 ALPVNIPGSRLYRSLQAKRNMVKLVRKIIQERKS--SGISPIPKDVMEVFLKDESEQLTD 274
           +LP+ +PG+RLYRSLQAK+ M +L+++II+E+++  +  SP P+D ++V + D S++LTD
Sbjct: 228 SLPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASP-PRDAIDVLIGDGSDELTD 287

Query: 275 DLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLHWS 334
           +LI++NMID+MIP EDSVP+L+TLAVK+LS+CP AL QL EEN+++KR K  +GE+L W+
Sbjct: 288 ELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGETLQWT 347

Query: 335 DYLSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHY 394
           DY+SL FTQ VITETLR+GNII G+MRKA++D+++KG+LIPKGW V  YFRSVHLDD  Y
Sbjct: 348 DYMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLDDTLY 407

Query: 395 DWPYKFNPWRWQDRDLSSCYFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEEDR 454
           D PYKFNPWRW+++D+S+  FTPFGGGQRLCPGLDLARLEASIFLHH VT FRW+AEED 
Sbjct: 408 DEPYKFNPWRWKEKDMSNGSFTPFGGGQRLCPGLDLARLEASIFLHHLVTSFRWVAEEDH 467

Query: 455 IINFPTVRMKKRMPVWVKIRRD 474
           I+NFPTVR+K+ MP+ V  + D
Sbjct: 468 IVNFPTVRLKRGMPIRVTAKED 488

BLAST of Tan0020131 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 521.9 bits (1343), Expect = 7.4e-147
Identity = 247/441 (56.01%), Postives = 340/441 (77.10%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSK 95
           +P GSLGWP IGET+ FI+C YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSQLKARITKDME 155
            +LQ+    FVP+YPKS+TEL+G++SIL ING  QKR+H L+GAF +S  LK RIT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 156 CYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V L++ +W +   +++QDE K ++F++LVK+L+S +PG+ M  LK +F+EFI GL+ +
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICI 248

Query: 216 PVNIPGSRLYRSLQAKRNMVKLVRKIIQERKSSGISPIP-KDVMEVFLKD----ESEQLT 275
           P+  PG+RLY+SL+AK  ++K+V+K+++ER+ +  +  P  DV++V L+D    E +   
Sbjct: 249 PIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQP 308

Query: 276 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLHW 335
            D ++  +++MMIPGE+++P  MTLAVK+LSD P AL +L EEN++MKR K +LGE   W
Sbjct: 309 SDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKW 368

Query: 336 SDYLSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 395
           +DY+SL FTQ+VI ETLRM NII GV RKA+KD++IKGYLIPKGW VL  F SVH+D++ 
Sbjct: 369 TDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDI 428

Query: 396 YDWPYKFNPWRW---QDRDLSSCYFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIA 455
           YD PY+F+PWRW        SS  FTPFGGGQRLCPGL+L++LE SIFLHH VT++ W A
Sbjct: 429 YDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWTA 488

Query: 456 EEDRIINFPTVRMKKRMPVWV 469
           EED I++FPTV+MK+R+P+ V
Sbjct: 489 EEDEIVSFPTVKMKRRLPIRV 509

BLAST of Tan0020131 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 8.5e-103
Identity = 192/444 (43.24%), Postives = 292/444 (65.77%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSQLKARITKDME 155
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 CYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V+ ++++W+    + L +EAK I+F++ VK L+S +PG+  E L++++   I G  +L
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 216 PVNIPGSRLYRSLQAKRNMVKLVRKIIQERKSSGI--SPIPKDVMEVFLKDESEQLTDDL 275
           P+ +  +   +++QA+R + + +  ++ +R+      +   KD++   L  + +  +D+ 
Sbjct: 211 PLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAAD-DGFSDEE 270

Query: 276 IAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLHWSDY 335
           I + ++ +++ G ++   +MTLAVK+L++ P AL QL EE+ K++ +K     SL WSDY
Sbjct: 271 IVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSDY 330

Query: 336 LSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDW 395
            S+PFTQ V+ ETLR+ NII GV R+AM D++IKGY IPKGW V + FR+VHLD NH+  
Sbjct: 331 KSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKD 390

Query: 396 PYKFNPWRWQDRDLS---SCYFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWI-AEE 455
              FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+ AE+
Sbjct: 391 ARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPAEQ 450

Query: 456 DRIINFPTVRMKKRMPVWVKIRRD 474
           D+++ FPT R +KR P++VK RRD
Sbjct: 451 DKLVFFPTTRTQKRYPIFVK-RRD 469

BLAST of Tan0020131 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 330.9 bits (847), Expect = 2.4e-89
Identity = 174/491 (35.44%), Postives = 296/491 (60.29%), Query Frame = 0

Query: 17  TIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVF 76
           +++L+    +    K    LP G  GWPF+GETI ++    +     FM +    YGK++
Sbjct: 20  SLLLFLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIY 79

Query: 77  KSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGL 136
           +S++FG PTIVS DA +++FILQ++ + F  SYP+S+  ++GK S+L++ G + + +  +
Sbjct: 80  RSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSI 139

Query: 137 VGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPG- 196
              F   ++L+  + KD+E +    +++W +N+    QDEAK  +F ++ K ++S++PG 
Sbjct: 140 SLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGE 199

Query: 197 QRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRKIIQERKSSGISPIPK 256
           +  E LK+++  F+ G+++ P+N+PG+  +++LQ++  ++K + + ++ERK   I    +
Sbjct: 200 EETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLD-IKEEDQ 259

Query: 257 DVMEVFLKDESE---------QLTDD-----------LIAENMIDMMI----PGEDSVPI 316
           +  EV  +DE+E         Q TDD           L  E ++D+++     G ++  +
Sbjct: 260 EEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSV 319

Query: 317 LMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGES-LHWSDYLSLPFTQSVITETLRMG 376
            + LA+ +L  CP A+++L EE+L++ R K++LGES L+W DY  + FTQ VI ETLR+G
Sbjct: 320 AIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLG 379

Query: 377 NIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSC 436
           N++  + RKA+KD++ KGY IP GW VL    +VHLD++ YD P  FNPWRWQ ++  + 
Sbjct: 380 NVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGAS 439

Query: 437 ------------YFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRW-IAEEDRIINFPT 469
                        + PFGGG RLC G +LA+LE ++F+HH V +F W +AE+D+   FP 
Sbjct: 440 SSGSGSFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKFNWELAEDDKPFAFPF 499

BLAST of Tan0020131 vs. NCBI nr
Match: XP_008462361.1 (PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo])

HSP 1 Score: 883.2 bits (2281), Expect = 9.6e-253
Identity = 440/474 (92.83%), Postives = 455/474 (95.99%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVT I   TIILY NCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRR+LYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKA+ITKDMECYVKLSM +WTENNPIYLQDE KNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRK 240
           SFQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPVNIPGSRLY+SLQAKRNMVKLV+K
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS +PKDVMEV LKDE+E+LTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMK  KEQLGE+LHWSDYLS+PFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFGGGQR 420
           MKDMKIKGYLI KGWSVLTYFRSVHLDDNHYD PYKFNPWRWQ+R+LSSC FTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKIRRDP 475
           LCPGLDLARLEASIFLHHFVT FRWIAEED+IINFPTVRMKKRMPVWVK RRDP
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 471

BLAST of Tan0020131 vs. NCBI nr
Match: XP_038898401.1 (LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida])

HSP 1 Score: 844.3 bits (2180), Expect = 5.0e-241
Identity = 420/460 (91.30%), Postives = 440/460 (95.65%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVT IFFTTIILY NCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFM+KRR+LYGKVFKSHIFGSPTIVSTD+EVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMNKRRRLYGKVFKSHIFGSPTIVSTDSEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKA+ITKDMECYV+LSM NWTENN IYLQDE KNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVQLSMANWTENNLIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRK 240
           SFQ+LVKILISLNPGQRMEFLK+QFQ+FIAGLMALPVNIPGSRLY+SLQAKRNMVKLV+K
Sbjct: 181 SFQILVKILISLNPGQRMEFLKKQFQDFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GISP+PKDVMEV LKDE+E+LTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISPVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMK LKEQLGE+LHWSDYLS+PFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGLKEQLGETLHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFGGGQR 420
           MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQDRDLSSC FTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDRDLSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRM 461
           LCPGLDLARLEASIFLHHFVT FR+    D   + P+VR+
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRL 459

BLAST of Tan0020131 vs. NCBI nr
Match: KAG6575481.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 844.0 bits (2179), Expect = 6.5e-241
Identity = 422/504 (83.73%), Postives = 448/504 (88.89%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDS WDV+V+T IF T I+LY NCF LLRSK C+ LPLGSLGWPFIGET+EFI+CAYSDR
Sbjct: 1   MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PE FMDKRR+LYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKA+ITKDMECYVKL+M NW+EN+P+YLQDE KNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSENSPVYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRS------------- 240
           SFQVLVKILISLNPGQRMEF+KQQFQEFIAGLMALPVNIPGSRLY+S             
Sbjct: 181 SFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALPSS 240

Query: 241 ---------------LQAKRNMVKLVRKIIQERKSSGISP--IPKDVMEVFLKDESEQLT 300
                          LQAKR MVKLV+KIIQER+S G SP  +P+DVMEV LKDESE LT
Sbjct: 241 FTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESELLT 300

Query: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLHW 360
           DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCP ALQQLTEENLKMKRLKEQ GE+LHW
Sbjct: 301 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALHW 360

Query: 361 SDYLSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 420
           SDYLS+PFTQ+VITETLRMGNIIIGVMRKAMKDM IKGYLIPKGWSVLTYFRSVHLDD+H
Sbjct: 361 SDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDSH 420

Query: 421 YDWPYKFNPWRWQDRDLSSCYFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEED 475
           Y  PYKFNPWRWQDRDLSSC FTPFGGGQRLCPGLDLARLEASIFLHHFVT+FRW+AEED
Sbjct: 421 YHCPYKFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTRFRWVAEED 480

BLAST of Tan0020131 vs. NCBI nr
Match: XP_011659645.1 (LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus])

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-239
Identity = 420/466 (90.13%), Postives = 438/466 (93.99%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVT IFFTTIILY NCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRR+LYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKA+ITKDMECYVKLSM NWTENNPIYLQDE KNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRK 240
           SFQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPVNIPGSRLY+SLQAKRNMVKLV+K
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS +PKD+MEV LKDE+E+LTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMK  KEQLGE+LHWSDYLS+PFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFGGGQR 420
           MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQDR++SSC FTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPV 467
           LCPGLDLARLEASIFLHHFVT FR+    D   + P+VR     P+
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTXLPPL 465

BLAST of Tan0020131 vs. NCBI nr
Match: KAE8646515.1 (hypothetical protein Csa_016429 [Cucumis sativus])

HSP 1 Score: 837.4 bits (2162), Expect = 6.1e-239
Identity = 415/444 (93.47%), Postives = 429/444 (96.62%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVT IFFTTIILY NCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRR+LYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKA+ITKDMECYVKLSM NWTENNPIYLQDE KNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRK 240
           SFQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPVNIPGSRLY+SLQAKRNMVKLV+K
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS +PKD+MEV LKDE+E+LTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMK  KEQLGE+LHWSDYLS+PFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFGGGQR 420
           MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQDR++SSC FTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTQFR 445
           LCPGLDLARLEASIFLHHFVT FR
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFR 444

BLAST of Tan0020131 vs. ExPASy TrEMBL
Match: A0A0A0K7W8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.1e-257
Identity = 443/474 (93.46%), Postives = 458/474 (96.62%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVT IFFTTIILY NCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRR+LYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKA+ITKDMECYVKLSM NWTENNPIYLQDE KNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRK 240
           SFQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPVNIPGSRLY+SLQAKRNMVKLV+K
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS +PKD+MEV LKDE+E+LTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMK  KEQLGE+LHWSDYLS+PFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFGGGQR 420
           MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQDR++SSC FTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKIRRDP 475
           LCPGLDLARLEASIFLHHFVT FRWIAEED+IINFPTVRMKKRMPVWVK RRDP
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 474

BLAST of Tan0020131 vs. ExPASy TrEMBL
Match: A0A1S3CGU6 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103500734 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.7e-253
Identity = 440/474 (92.83%), Postives = 455/474 (95.99%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVT I   TIILY NCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRR+LYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKA+ITKDMECYVKLSM +WTENNPIYLQDE KNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRK 240
           SFQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPVNIPGSRLY+SLQAKRNMVKLV+K
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS +PKDVMEV LKDE+E+LTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMK  KEQLGE+LHWSDYLS+PFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFGGGQR 420
           MKDMKIKGYLI KGWSVLTYFRSVHLDDNHYD PYKFNPWRWQ+R+LSSC FTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKIRRDP 475
           LCPGLDLARLEASIFLHHFVT FRWIAEED+IINFPTVRMKKRMPVWVK RRDP
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 471

BLAST of Tan0020131 vs. ExPASy TrEMBL
Match: E5GCA5 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 6.8e-236
Identity = 417/466 (89.48%), Postives = 435/466 (93.35%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVT I   TIILY NCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRR+LYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKA+ITKDMECYVKLSM +WTENNPIYLQDE KNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRK 240
           SFQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPVNIPGSRLY+SLQAKRNMVKLV+K
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS +PKDVMEV LKDE+E+LTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMK  KEQLGE+LHWSDYLS+PFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFGGGQR 420
           MKDMKIKGYLI KGWSVLTYFRSVHLDDNHYD PYKFNPWRWQ+R+LSSC FTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPV 467
           LCPGLDLARLEASIFLHHFVT FR+    D   + P+VR     P+
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTNFPPL 462

BLAST of Tan0020131 vs. ExPASy TrEMBL
Match: A0A5A7UW93 (Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001660 PE=3 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.6e-235
Identity = 418/470 (88.94%), Postives = 436/470 (92.77%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVT I   TIILY NCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRR+LYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SILLINGSLQKRIHGLVGAFFKSS LKA+ITKDMECYVKLSM +WTENNPIYLQDE KNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQ----AKRNMVK 240
           SFQVLVKILISLNPGQ MEFLK+QFQEFIAGLMALPVNIPGSRLY+SLQ    AKRNMVK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQASWTAKRNMVK 240

Query: 241 LVRKIIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLA 300
           LV+KIIQERKS GIS +PKDVMEV LKDE+E+LTDDLIAENMIDMMIPGEDSVPILMTLA
Sbjct: 241 LVKKIIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLA 300

Query: 301 VKYLSDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGV 360
           VKYLSDCPAALQQLTEENLKMK  KEQLGE+LHWSDYLS+PFTQSVITETLRMGNIIIGV
Sbjct: 301 VKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGV 360

Query: 361 MRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFG 420
           MRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQ+R+LSSC FTPFG
Sbjct: 361 MRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFG 420

Query: 421 GGQRLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPV 467
           GGQRLCPGLDLARLEASIFLHHFVT FR+    D   + P+VR     P+
Sbjct: 421 GGQRLCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTNFPPL 466

BLAST of Tan0020131 vs. ExPASy TrEMBL
Match: A0A7N2LKP8 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 6.8e-220
Identity = 379/471 (80.47%), Postives = 426/471 (90.45%), Query Frame = 0

Query: 1   MDSLWDVVVVTTIFFTTIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MD++W V++VTTIF +TIILY N FR LRSK  + LPLG+LG PFIGETIEF+SCAYSDR
Sbjct: 1   MDNMW-VLLVTTIFLSTIILYKNRFR-LRSKHRSPLPLGTLGLPFIGETIEFVSCAYSDR 60

Query: 61  PETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PE+FMDKRR +YGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PESFMDKRRSMYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNI 180
           SIL+INGSLQKRIHGL+GAFFKS  LKA+IT+DM  YV+ SM NW +++PIY+QDE KNI
Sbjct: 121 SILVINGSLQKRIHGLIGAFFKSPHLKAQITRDMHKYVQESMANWKDDHPIYMQDETKNI 180

Query: 181 SFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRK 240
           +FQVLVK LISL+PG+ MEFLK+QFQEFI+GLM+LP+NIPG+RLYRSLQAK+ MVKLV+ 
Sbjct: 181 AFQVLVKALISLDPGEEMEFLKKQFQEFISGLMSLPINIPGTRLYRSLQAKKKMVKLVQN 240

Query: 241 IIQERKSSGISPIPKDVMEVFLKDESEQLTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           +I  RK SG S +PKDV++V L D SEQLT+DLIA+NMIDMMIPGEDSVP+LMTLA+KYL
Sbjct: 241 VILARKHSGFSNVPKDVVDVLLNDSSEQLTEDLIADNMIDMMIPGEDSVPVLMTLAIKYL 300

Query: 301 SDCPAALQQLTEENLKMKRLKEQLGESLHWSDYLSLPFTQSVITETLRMGNIIIGVMRKA 360
           SDCP ALQQLTEENLK+K LK QLG+ L+WSDYLSL FTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPTALQQLTEENLKLKELKAQLGQPLYWSDYLSLSFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCYFTPFGGGQR 420
           MKD++IKGYLIPKGW V  YFRSVHLD NHYDWPYKFNPWRWQD+D+SS  FTPFGGGQR
Sbjct: 361 MKDIEIKGYLIPKGWCVFAYFRSVHLDANHYDWPYKFNPWRWQDKDMSSSNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKIR 472
           LCPGLDLARLEASIFLHHFVTQF W AEED I+NFPTVRMK+RMP+WVK R
Sbjct: 421 LCPGLDLARLEASIFLHHFVTQFSWRAEEDTIVNFPTVRMKRRMPIWVKRR 469

BLAST of Tan0020131 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 614.0 bits (1582), Expect = 1.0e-175
Identity = 294/440 (66.82%), Postives = 363/440 (82.50%), Query Frame = 0

Query: 35  QLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVS 94
           + P GSLGWP IGETIEF+S AYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV+
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 95  KFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSQLKARITKDM 154
           + +LQSD+ AFVP YPK++ ELMGKSSILLINGSL +R HGLVG+F KS  LKA+I +DM
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 155 ECYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMA 214
             ++  SM+ W+E+ P+ LQD +K ++F+VL K LIS+  G+ +E LK++F+ FI+GLM+
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 215 LPVNIPGSRLYRSLQAKRNMVKLVRKIIQ-----ERKSSGISPIPKDVMEVFLKDESEQL 274
           LP+N PG++L+RSLQAK+NMVK V +II+      +       I KDV++V LKD SE L
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHL 289

Query: 275 TDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLH 334
           T +LIA NMIDMMIPG DSVP+L+TLAVK+LSD PAAL  LTEEN+K+K LKE  GE L+
Sbjct: 290 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 349

Query: 335 WSDYLSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDN 394
           W+DYLSLPFTQ VITETLRMGN+IIGVMRKAMKD++IKGY+IPKGW  L Y RSVHLD  
Sbjct: 350 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 409

Query: 395 HYDWPYKFNPWRWQDRDLSSCYFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEE 454
           +Y+ PYKFNPWRWQ+RD+++  F+PFGGGQRLCPGLDLARLE S+FLHH VT+FRWIAEE
Sbjct: 410 YYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEE 469

Query: 455 DRIINFPTVRMKKRMPVWVK 470
           D IINFPTV MK ++P+W+K
Sbjct: 470 DTIINFPTVHMKNKLPIWIK 489

BLAST of Tan0020131 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 521.9 bits (1343), Expect = 5.2e-148
Identity = 247/441 (56.01%), Postives = 340/441 (77.10%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSK 95
           +P GSLGWP IGET+ FI+C YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSQLKARITKDME 155
            +LQ+    FVP+YPKS+TEL+G++SIL ING  QKR+H L+GAF +S  LK RIT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 156 CYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V L++ +W +   +++QDE K ++F++LVK+L+S +PG+ M  LK +F+EFI GL+ +
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICI 248

Query: 216 PVNIPGSRLYRSLQAKRNMVKLVRKIIQERKSSGISPIP-KDVMEVFLKD----ESEQLT 275
           P+  PG+RLY+SL+AK  ++K+V+K+++ER+ +  +  P  DV++V L+D    E +   
Sbjct: 249 PIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQP 308

Query: 276 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLHW 335
            D ++  +++MMIPGE+++P  MTLAVK+LSD P AL +L EEN++MKR K +LGE   W
Sbjct: 309 SDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKW 368

Query: 336 SDYLSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 395
           +DY+SL FTQ+VI ETLRM NII GV RKA+KD++IKGYLIPKGW VL  F SVH+D++ 
Sbjct: 369 TDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDI 428

Query: 396 YDWPYKFNPWRW---QDRDLSSCYFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIA 455
           YD PY+F+PWRW        SS  FTPFGGGQRLCPGL+L++LE SIFLHH VT++ W A
Sbjct: 429 YDNPYQFDPWRWDRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWTA 488

Query: 456 EEDRIINFPTVRMKKRMPVWV 469
           EED I++FPTV+MK+R+P+ V
Sbjct: 489 EEDEIVSFPTVKMKRRLPIRV 509

BLAST of Tan0020131 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 375.6 bits (963), Expect = 6.1e-104
Identity = 192/444 (43.24%), Postives = 292/444 (65.77%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSQLKARITKDME 155
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 CYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V+ ++++W+    + L +EAK I+F++ VK L+S +PG+  E L++++   I G  +L
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 216 PVNIPGSRLYRSLQAKRNMVKLVRKIIQERKSSGI--SPIPKDVMEVFLKDESEQLTDDL 275
           P+ +  +   +++QA+R + + +  ++ +R+      +   KD++   L  + +  +D+ 
Sbjct: 211 PLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAAD-DGFSDEE 270

Query: 276 IAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLHWSDY 335
           I + ++ +++ G ++   +MTLAVK+L++ P AL QL EE+ K++ +K     SL WSDY
Sbjct: 271 IVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSDY 330

Query: 336 LSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDW 395
            S+PFTQ V+ ETLR+ NII GV R+AM D++IKGY IPKGW V + FR+VHLD NH+  
Sbjct: 331 KSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKD 390

Query: 396 PYKFNPWRWQDRDLS---SCYFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWI-AEE 455
              FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+ AE+
Sbjct: 391 ARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPAEQ 450

Query: 456 DRIINFPTVRMKKRMPVWVKIRRD 474
           D+++ FPT R +KR P++VK RRD
Sbjct: 451 DKLVFFPTTRTQKRYPIFVK-RRD 469

BLAST of Tan0020131 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.7e-90
Identity = 174/491 (35.44%), Postives = 296/491 (60.29%), Query Frame = 0

Query: 17  TIILYTNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVF 76
           +++L+    +    K    LP G  GWPF+GETI ++    +     FM +    YGK++
Sbjct: 20  SLLLFLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIY 79

Query: 77  KSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGL 136
           +S++FG PTIVS DA +++FILQ++ + F  SYP+S+  ++GK S+L++ G + + +  +
Sbjct: 80  RSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSI 139

Query: 137 VGAFFKSSQLKARITKDMECYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPG- 196
              F   ++L+  + KD+E +    +++W +N+    QDEAK  +F ++ K ++S++PG 
Sbjct: 140 SLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGE 199

Query: 197 QRMEFLKQQFQEFIAGLMALPVNIPGSRLYRSLQAKRNMVKLVRKIIQERKSSGISPIPK 256
           +  E LK+++  F+ G+++ P+N+PG+  +++LQ++  ++K + + ++ERK   I    +
Sbjct: 200 EETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLD-IKEEDQ 259

Query: 257 DVMEVFLKDESE---------QLTDD-----------LIAENMIDMMI----PGEDSVPI 316
           +  EV  +DE+E         Q TDD           L  E ++D+++     G ++  +
Sbjct: 260 EEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSV 319

Query: 317 LMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGES-LHWSDYLSLPFTQSVITETLRMG 376
            + LA+ +L  CP A+++L EE+L++ R K++LGES L+W DY  + FTQ VI ETLR+G
Sbjct: 320 AIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLG 379

Query: 377 NIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSC 436
           N++  + RKA+KD++ KGY IP GW VL    +VHLD++ YD P  FNPWRWQ ++  + 
Sbjct: 380 NVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGAS 439

Query: 437 ------------YFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRW-IAEEDRIINFPT 469
                        + PFGGG RLC G +LA+LE ++F+HH V +F W +AE+D+   FP 
Sbjct: 440 SSGSGSFSTWGNNYMPFGGGPRLCAGSELAKLEMAVFIHHLVLKFNWELAEDDKPFAFPF 499

BLAST of Tan0020131 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 300.8 bits (769), Expect = 1.9e-81
Identity = 153/370 (41.35%), Postives = 240/370 (64.86%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRQLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSQLKARITKDME 155
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 CYVKLSMENWTENNPIYLQDEAKNISFQVLVKILISLNPGQRMEFLKQQFQEFIAGLMAL 215
             V+ ++++W+    + L +EAK I+F++ VK L+S +PG+  E L++++   I G  +L
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 216 PVNIPGSRLYRSLQAKRNMVKLVRKIIQERKSSGI--SPIPKDVMEVFLKDESEQLTDDL 275
           P+ +  +   +++QA+R + + +  ++ +R+      +   KD++   L  + +  +D+ 
Sbjct: 211 PLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAAD-DGFSDEE 270

Query: 276 IAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKRLKEQLGESLHWSDY 335
           I + ++ +++ G ++   +MTLAVK+L++ P AL QL EE+ K++ +K     SL WSDY
Sbjct: 271 IVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSDY 330

Query: 336 LSLPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDW 395
            S+PFTQ V+ ETLR+ NII GV R+AM D++IKGY IPKGW V + FR+VHLD NH+  
Sbjct: 331 KSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKD 390

Query: 396 PYKFNPWRWQ 404
              FNPWRWQ
Sbjct: 391 ARTFNPWRWQ 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94IA61.4e-17466.823-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37... [more]
Q94IW54.8e-17063.35Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV... [more]
Q9M0667.4e-14756.013-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37... [more]
Q425698.5e-10343.24Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
O649892.4e-8935.44Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_008462361.19.6e-25392.83PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo][more]
XP_038898401.15.0e-24191.30LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida][more]
KAG6575481.16.5e-24183.733-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrospe... [more]
XP_011659645.11.2e-23990.13LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis... [more]
KAE8646515.16.1e-23993.47hypothetical protein Csa_016429 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A0A0K7W84.1e-25793.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1[more]
A0A1S3CGU64.7e-25392.833-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC1035007... [more]
E5GCA56.8e-23689.48Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A5A7UW932.6e-23588.94Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G0016... [more]
A0A7N2LKP86.8e-22080.47Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13730.11.0e-17566.82cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
AT4G36380.15.2e-14856.01Cytochrome P450 superfamily protein [more]
AT5G05690.16.1e-10443.24Cytochrome P450 superfamily protein [more]
AT3G50660.11.7e-9035.44Cytochrome P450 superfamily protein [more]
AT5G05690.21.9e-8141.35Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 307..327
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 9..444
e-value: 1.2E-10
score: 38.7
NoneNo IPR availablePANTHERPTHR24286:SF303-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1coord: 19..466
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 19..466
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 412..422
score: 51.88
coord: 69..88
score: 25.99
coord: 422..445
score: 32.35
coord: 340..358
score: 24.05
coord: 380..404
score: 26.33
IPR001128Cytochrome P450PFAMPF00067p450coord: 37..450
e-value: 3.0E-55
score: 187.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 30..471
e-value: 9.2E-98
score: 329.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 35..471
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 415..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0020131.1Tan0020131.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen