Tan0020109 (gene) Snake gourd v1

Overview
NameTan0020109
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionvesicle-associated protein 2-2-like
LocationLG08: 4710159 .. 4716110 (+)
RNA-Seq ExpressionTan0020109
SyntenyTan0020109
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAACCTCGAAATTGGAGCCAAGTCCGTTAAAATTATTTGAAAAAAAAAAGTTCAAACGGGAATTGGATTCTGCCGCACCGGCTCAAAGCTAAGATCACAGCACAGCCGGTACAACTAACACATCTCTCTCTCTCTCTTCTTTCTCTCTCTACTTTGCTTTCACAGAGCAGTCTTCCAGACCACCAAACAGATCATTCATTCACGTTTCTCTCTCTCCCTCTCTAGAAATTTCAGGGTTTCTCTCTCCTTATGAAATTGTAAATTCCACCAAATCTTTGGACGCTATTACAATTTCAGTTTCTCAGTGCGTTTTATTTATTCAAAGCGGAATTTTCACGCCGAAGTTTCCATTCTTCTCCACTTCTCTTTGTTCTTTGTCTTTTGGGTCTCTTGGAGTTTCTTCTCCTGCGGAACGGGTGAGTTTTTGGATTCTTTTGTGCATTTTTCTGAAGACTGGAAGAGTGGAAGAGTATTCTTGTTTGACTGCTTTGTTGGGTTTATTGTTCGAAATTTCAGTTAGCGTGTTGAGAACGTAGTCTGTGTGTATTTTGTTGAATTTCTGCTGCTTTCTTGAGTATTGGAGTTTTTTAGTTTCGGAATTGGGTGTGATTCTGGTGTTTGTTTACGATCGGAGGTTGATGTTGTTGATCTTTTTGGTTTGTTGAGTATGGAAATGGGTCGGAATTTGTAATTGGATTTGTGTTTAGAGCGACTCTATTCGTTGATTAATGATTTACTGCTCGAGACGTGTTTATTTTGAACGATCTGTTTTGGAATTAGCTCAGCGGTGGGTCTCTGATCCTCACTGAAAACGGGTTTGGTTTGACTTTCTTGTGGTTGACTGACCGATACAAAATGGAACCCAGTTTTTTTTTTTTCGAAAATTGCGGCATGGAGACATTATTATTATTTTCTTTTCGCTAAGAACAGGGTAGATGGGATGCACGTGGCCCTTAAGTTCTTTGATCTGTAATTATATGATGGAATACTTGAGATTTTATGTTTCTTTCTTTTCTTTCCTTCGATTGCTTCTTTATCAAATCCAAATTGTTTGTCCACGATTATAAGAACATTACTTTTCTGAGTGCAGAATAATTGTTGCCTAAGCTTTGGATGCAGAGAAGTATTACTTTTGAATTTGGTGTATTGTGACAAATAAACATGAGTACGAAACTCTTGCATATCCAGCCCAAGGAATTACAATTCATATGTAAGTGCATCATCAGTAATACTAGTTAAGTAATTGCTTGCTCTTGAAAAAGAATTGAAGCGAAGTACCTTGCTGCTTTCAGTTGAACTGAGGAAACAAAGTACGTGCACAGTTCAACTAGCCAATAACACCCATCATCATGTTGCCTTTAAAGTATGAACAACCTTCTTTTTGTTTTGTTTGTCTTGTGTTTCTCTGAAATTTATGTGATTTTTCATGTGATATATGTAATAATATTATAACGTTTGGCAGGTTAAAACTACATCTCCTAAGAAATACTGTGTTCGACCAAATGTTGGGATAATTTTTCCAAAATCAACATGTGAATTTTCAGGTATGTTCTGTACAAATTTCATCGAACATATTCAGGCACCTGAAAGAATTGACGACCCCCCCCCCCCCGGGGCAAAAAAGAAACTGTATATAGATATCGGTGATGAAGTTACTGATGCCGCCATTCAATTTTAACATTTGAGTTTCAATGAAATTAATTCTTCCGTGCATTACTTTACATTTTCACTATCTGATTTGCTCAATTTCTTTCCCAAAAGGGAAATTTGAAAGCATAACATTCATTTTTTGAGGATGTAAAACTCTATGAATGTCTAATATCATGAGATGATTCTGTTCTGCATTTGACAATTTTTTGTCCCATCAACTTTTTTAGCAAGACTGTGTTTAGTCTTCTTAAAGAATTTATTGTGCCGTTGAAGTGAGGACTTATGAGATATCATGAAATCCCACTCCATAGTGTATGCAATAAGGTTAACTGTATTCCAGTAATACTTGTATCGATTTTTTTTTTTTTTAAATTCTAAACTTGGGAACTATATATTCTTACTTACCTTTTTTTCCTTTTTTTTAACGTTAATTTCTTCTTATGATTCTGATGTAGTTATAATGCAAGCTCAACGGGCTGCCCCACCTGATATGTTCTGTAAAGACAAATTTTTAATTCAAAGCACAATTGTTCCTTCAGGGACAACAGAAGAGGACATCACTGCAAGTATGGTAAGTTTTTGTGGATATAATAAGCTTTCATTCTTTGGGTTTTTAATTGCTTTCTATTTTTCTTTTTTTTAGTTTTCAAAAGATGGAGGTAAATATATTGAAGAGGATAAGCTCAAAGTAGCGCTCATTAGTCCACTGAATTCCCCAACACTCTCACCTACCGATGGAGCATTGGACACAATCATGGATGAAGTTGTGAAGCAGAACAAGAAAAGTGGACAATTAAGAGATGGGTTGGCCACCAATGAAACTCCAAAGTTGAAAGATCAAAATGATGCATCGGATGAGGTGCTTTCCTCTGGGGTTTCAACACTAAGAGATCAAAATGGACCATCAAATGACAGTATGGCCCATGAGGCTTCAAGATTCAAAGATGATATCGTTGCAGTGAAGGGGGGGCGGACCATTGAAGCTTTAAAACCATATGATCAAAATGGAGCATCAAGGGAGGAGGTTCTGCTTCATGAGACTTCAAGATTGAAGGATCAAAATGGAACATCCAAGGAGGAGCTACCTCGTGAAGCATTAACACCAAGCGCTCAAAATGGAGCATTAAAGGAGGAGGAGTTGAGTGCTGAAGCTCCAAAAAGAAGAGTTCAAAATGGAGCACAGGAGGGGCGAGCCTTTGAAGCTTCGCATCTGGAGGATCCATTACCAGACAATGTTATGGTTCAACCTCTGCCATCCGTAGTAAAAGTAAGGAAAAACATTCTTATCTATACAGAGTTAAAATTTCTCCTCTCTCTTATCTCTTTAAAAAATGTTCCCGAGGGATCAAATCGGTATTTTCCTTAATAAATTTTATGTTATACTGCTTAATTATTGTTGGTCAAAGAACTTGTTGGAGGTCTCTTTTTAACCTCTTCCACATTCCTAGGGAGGTTGCCCTATCTTTCTTTGTTTTTCCCAATAGAAAGTGTTTCTTATCTAGAAAAGGTGAATTTGTCGTAGCAGGAGATGGTAGAACAATCTGTTAACTTGGATAGTGTGTCACTAATTGGTCATTGTTCAATCTGATTGGTGGAACTGCCAATTGTAACGTTCTGTGATCTTCAGCTTCTTGAAAATTGAAGTTTTTTTCTTGTGAATTAGATTCCTAAGGGTGTGGATGAATACCTAGAGCTAAACAATAGCTCGTTGAGATTACAAAAGACTGCTGAGATAAAGCAAGCAGCAAAATTGGAGCCGATGACAGTGAAAAAAGTGGAACAGCTAAAGTTAGTTAAGGACATTGATGAGATGAAGTCTAAGCTAAATGAAATTGAATTAAAGCTTGGAGAGGTGAGTTTTGATTTCCTTTTGTGCTTTTCTCATTTTTGCAGCTTTTACATGTATGGATAATAATTGCGAATCTGGTCGTTGAGATTGTTTGTCCTTATCTAATCCAATCAAGAATTACGGCTCTGATTGATTTCTATTTTTTTTGTATTCAGTTTATTTTCTATGGAATTATGTACGAGTATGTACTATTAACATATCAGTTGATTTGTAGAAAGAACCAGTGAACAATAAGATCTCAAACAATTTTTAGATAAAAAAACTGAATATCATTCCGAAGTAAAGAGATGTAGAATAGAGGGAAAACCTCAAAAGCCAAGTAGAATATTAAGAAACTCCCACCATTTGGCATGGATATAAAGAAATAACCAGTTTAGAAAGAGAGCACCATAAAGAAGCCAAAAATTCAACATTGTCCCAAAAATTACTAAAATCTTTTATGTCAATCCCCAGAAGTTCTGAATTCTGTTTGTCAAACATCAACTTCTGATGAGATTCACCTTACCCCGTTGCAATCCTGGAGTCTCAAAACTCGTCTTTCAGGCATGGGTTATAAGGCTCCTTGGGTTGGATGTTCAATTATGCAACTTTTGTGATTGATTTTGATTTTTCACTGCATTTTCTACTTTGTTTTGTTCTACAGATTGATTCTTAAATTTTGTTAAAACTTGGGTTTCGGTTTATCAGCTGTTTATTATTATTCCTTCAAAGTTCAAAATGTTTTTCCATAATTAGAGAAAGAGACAGGTAAAGACATCATTTTATCATAATCTCAGTACCCGGAAATGAATCGTTGATGGAAATGGAAAAAACTACTTTCCTAATATGCATTTCTCCATTTGCTGTATTATTTTGCTTTTTATGGACTGTCCAATACAATTGTAATGATGTTCTTTTGTCGCATCATTTAATAGGCTCAAGGTACCATTTCAATGCTGTCCGAGGAGAGGAGATTAAGTGCTCAAGAAGTAAAGAACTTGAAGGAGAAATTGGTAACTTTCTGGACCATATTACTCTGTTAGTGGATGTTCTTGTTGCATGTTCAGAGTCTTATGTTTGCCACTGCCCTGTGACTGTATCTTGTCTATTTGATAGCAATATTCAAGCCTGGTTACCTCAAGGGTACCCTAGTACTAGAGAGATTAGAGACTTCTTGCTAGATTCAAAAGGGTAGGGTAGCTTTACCCTAGGTACCTGCTGTCGTTATCTTGGTGGTGGTAAGGATTAGAGACTTTTTGCCCTATTCAAAAGGATAGGGAAGCTTAACCCTAGGTACCCACCGACAGTATCTTGGTGGTAAGGATTTGATATGTTTGGAGATGTCTTTAAGATCCTAGGTTGAAACTTCTTAGTTTGAAAATTTAAAATCCTTGTGGTCTTTTGGAACTGACTTTGGAGTGGATGTGGATGAAGTCTTCTTAAAGATTTTGAGGTGTGCTGCGAGCAGAATAGAAAGATTGGATGAAGTCTTCTTACAGATTTAGTTGGAGGTGTGCTATGAGCATAATAAAAGGATTATAGGGTGTCAACATCCCAGTTAATCCCAGAAAGAAAAAAAAGGAAGGAAAGCGCCCCGTCTGAACATAGCTCAACGGGTTAGGGATACACAATCGGCCCAAAGGTTAGAGATTCTAATTCTCGTCTCAACATGTTGTTTTAACTCAAAAAAGAGAGAGAGAAAAAGAGAAAAAGAAAAGGGGTAGCTTTTAGTGGAGCCAAAAGGAAAACCACTTCTTTTATTTATGTTTTCTCTTAAAGTAAAGATTACTATTCAATTGAGTTGCTTTGATATTTTTGAAATACTACATAGAAATTCATTCATCCTGCCCATTGAACTGTACTTTGAAACGCAGTTTCTGTATACTACAATTGAATTTCAGCTAGTTCCTTAATACTTGTATTTAAGATTCCTCTCGCTTCTCTGTCTTTCTTCCCTACATTTCGTTACTCCATGTTTCATCTTACGATTTTCCCAGATGGAATTGAGTAGGAGAGACAGGGCGAGCAATCAAGTCGGTTTTCCTCCTTTGTTTATCTGTATGGTGGCGCTTATTTGCATTGTGCTCGGATACGTTTTCCACTATTGAAGAACCATGTGCGGCGATCCCGAGTGATTGAAGATTGGAGAAAACATGTAAAGGAATATCTTACTTATGTAGATAGATTGGTAGCTAGTGCTGTGTGTTGTCAATAAGAATAGCCCCCATTCACAATCACAACACACGCCAAAAGATGCACATTTTGATTTTGGCTACTAGAATTTTGCTGCTTTTAGTATGCAATTCACATTTTGGGTATAATTGTAAGCTCGTCTGCTTTTAACTAACGTTTGAAGATAAGTGGTGGTGATCGACTAAATGTTGGCTTTCTGGTTGGTCTTCTCACTTTCTTTTTCCCTTTCTATGTGCAAACTTTTTACTAAATTTATGAAAATTTGATCTTATTTTGAA

mRNA sequence

AAGAAACCTCGAAATTGGAGCCAAGTCCGTTAAAATTATTTGAAAAAAAAAAGTTCAAACGGGAATTGGATTCTGCCGCACCGGCTCAAAGCTAAGATCACAGCACAGCCGGTACAACTAACACATCTCTCTCTCTCTCTTCTTTCTCTCTCTACTTTGCTTTCACAGAGCAGTCTTCCAGACCACCAAACAGATCATTCATTCACGTTTCTCTCTCTCCCTCTCTAGAAATTTCAGGGTTTCTCTCTCCTTATGAAATTGTAAATTCCACCAAATCTTTGGACGCTATTACAATTTCAGTTTCTCAGTGCGTTTTATTTATTCAAAGCGGAATTTTCACGCCGAAGTTTCCATTCTTCTCCACTTCTCTTTGTTCTTTGTCTTTTGGGTCTCTTGGAGTTTCTTCTCCTGCGGAACGGAATAATTGTTGCCTAAGCTTTGGATGCAGAGAAGTATTACTTTTGAATTTGGTGTATTGTGACAAATAAACATGAGTACGAAACTCTTGCATATCCAGCCCAAGGAATTACAATTCATATTTGAACTGAGGAAACAAAGTACGTGCACAGTTCAACTAGCCAATAACACCCATCATCATGTTGCCTTTAAAGTTAAAACTACATCTCCTAAGAAATACTGTGTTCGACCAAATGTTGGGATAATTTTTCCAAAATCAACATGTGAATTTTCAGTTATAATGCAAGCTCAACGGGCTGCCCCACCTGATATGTTCTGTAAAGACAAATTTTTAATTCAAAGCACAATTGTTCCTTCAGGGACAACAGAAGAGGACATCACTGCAAGTATGTTTTCAAAAGATGGAGGTAAATATATTGAAGAGGATAAGCTCAAAGTAGCGCTCATTAGTCCACTGAATTCCCCAACACTCTCACCTACCGATGGAGCATTGGACACAATCATGGATGAAGTTGTGAAGCAGAACAAGAAAAGTGGACAATTAAGAGATGGGTTGGCCACCAATGAAACTCCAAAGTTGAAAGATCAAAATGATGCATCGGATGAGGTGCTTTCCTCTGGGGTTTCAACACTAAGAGATCAAAATGGACCATCAAATGACAGTATGGCCCATGAGGCTTCAAGATTCAAAGATGATATCGTTGCAGTGAAGGGGGGGCGGACCATTGAAGCTTTAAAACCATATGATCAAAATGGAGCATCAAGGGAGGAGGTTCTGCTTCATGAGACTTCAAGATTGAAGGATCAAAATGGAACATCCAAGGAGGAGCTACCTCGTGAAGCATTAACACCAAGCGCTCAAAATGGAGCATTAAAGGAGGAGGAGTTGAGTGCTGAAGCTCCAAAAAGAAGAGTTCAAAATGGAGCACAGGAGGGGCGAGCCTTTGAAGCTTCGCATCTGGAGGATCCATTACCAGACAATGTTATGGTTCAACCTCTGCCATCCGTAGTAAAAATTCCTAAGGGTGTGGATGAATACCTAGAGCTAAACAATAGCTCGTTGAGATTACAAAAGACTGCTGAGATAAAGCAAGCAGCAAAATTGGAGCCGATGACAGTGAAAAAAGTGGAACAGCTAAAGTTAGTTAAGGACATTGATGAGATGAAGTCTAAGCTAAATGAAATTGAATTAAAGCTTGGAGAGGCTCAAGGTACCATTTCAATGCTGTCCGAGGAGAGGAGATTAAGTGCTCAAGAAGTAAAGAACTTGAAGGAGAAATTGATGGAATTGAGTAGGAGAGACAGGGCGAGCAATCAAGTCGGTTTTCCTCCTTTGTTTATCTGTATGGTGGCGCTTATTTGCATTGTGCTCGGATACGTTTTCCACTATTGAAGAACCATGTGCGGCGATCCCGAGTGATTGAAGATTGGAGAAAACATGTAAAGGAATATCTTACTTATGTAGATAGATTGGTAGCTAGTGCTGTGTGTTGTCAATAAGAATAGCCCCCATTCACAATCACAACACACGCCAAAAGATGCACATTTTGATTTTGGCTACTAGAATTTTGCTGCTTTTAGTATGCAATTCACATTTTGGGTATAATTGTAAGCTCGTCTGCTTTTAACTAACGTTTGAAGATAAGTGGTGGTGATCGACTAAATGTTGGCTTTCTGGTTGGTCTTCTCACTTTCTTTTTCCCTTTCTATGTGCAAACTTTTTACTAAATTTATGAAAATTTGATCTTATTTTGAA

Coding sequence (CDS)

ATGAGTACGAAACTCTTGCATATCCAGCCCAAGGAATTACAATTCATATTTGAACTGAGGAAACAAAGTACGTGCACAGTTCAACTAGCCAATAACACCCATCATCATGTTGCCTTTAAAGTTAAAACTACATCTCCTAAGAAATACTGTGTTCGACCAAATGTTGGGATAATTTTTCCAAAATCAACATGTGAATTTTCAGTTATAATGCAAGCTCAACGGGCTGCCCCACCTGATATGTTCTGTAAAGACAAATTTTTAATTCAAAGCACAATTGTTCCTTCAGGGACAACAGAAGAGGACATCACTGCAAGTATGTTTTCAAAAGATGGAGGTAAATATATTGAAGAGGATAAGCTCAAAGTAGCGCTCATTAGTCCACTGAATTCCCCAACACTCTCACCTACCGATGGAGCATTGGACACAATCATGGATGAAGTTGTGAAGCAGAACAAGAAAAGTGGACAATTAAGAGATGGGTTGGCCACCAATGAAACTCCAAAGTTGAAAGATCAAAATGATGCATCGGATGAGGTGCTTTCCTCTGGGGTTTCAACACTAAGAGATCAAAATGGACCATCAAATGACAGTATGGCCCATGAGGCTTCAAGATTCAAAGATGATATCGTTGCAGTGAAGGGGGGGCGGACCATTGAAGCTTTAAAACCATATGATCAAAATGGAGCATCAAGGGAGGAGGTTCTGCTTCATGAGACTTCAAGATTGAAGGATCAAAATGGAACATCCAAGGAGGAGCTACCTCGTGAAGCATTAACACCAAGCGCTCAAAATGGAGCATTAAAGGAGGAGGAGTTGAGTGCTGAAGCTCCAAAAAGAAGAGTTCAAAATGGAGCACAGGAGGGGCGAGCCTTTGAAGCTTCGCATCTGGAGGATCCATTACCAGACAATGTTATGGTTCAACCTCTGCCATCCGTAGTAAAAATTCCTAAGGGTGTGGATGAATACCTAGAGCTAAACAATAGCTCGTTGAGATTACAAAAGACTGCTGAGATAAAGCAAGCAGCAAAATTGGAGCCGATGACAGTGAAAAAAGTGGAACAGCTAAAGTTAGTTAAGGACATTGATGAGATGAAGTCTAAGCTAAATGAAATTGAATTAAAGCTTGGAGAGGCTCAAGGTACCATTTCAATGCTGTCCGAGGAGAGGAGATTAAGTGCTCAAGAAGTAAAGAACTTGAAGGAGAAATTGATGGAATTGAGTAGGAGAGACAGGGCGAGCAATCAAGTCGGTTTTCCTCCTTTGTTTATCTGTATGGTGGCGCTTATTTGCATTGTGCTCGGATACGTTTTCCACTATTGA

Protein sequence

MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFPKSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKLKVALISPLNSPTLSPTDGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEVLSSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHETSRLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFEASHLEDPLPDNVMVQPLPSVVKIPKGVDEYLELNNSSLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLSEERRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYVFHY
Homology
BLAST of Tan0020109 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.7e-60
Identity = 169/451 (37.47%), Postives = 238/451 (52.77%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           M+  LL IQP+ LQF  +L+KQ++C VQL N THH+VAFKVKTTSPKKYCVRPNVG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KSTCEF+VIMQA +  PPDM CKDKFLIQST V + TT+EDITASMFSK  GK+IEE+KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 KVALISPLNSPTLSPTDGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEVL 180
           +V L+ P +SP LSP +                            TPK            
Sbjct: 121 RVTLVPPSDSPELSPIN----------------------------TPK------------ 180

Query: 181 SSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHETS 240
                               + + F+D I+  +     E L P    G   +E  +    
Sbjct: 181 --------------------QGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 240

Query: 241 RLKDQNGTSKEELPREALTPSAQN-------GALKEEELSAEAPKR------RVQNGAQE 300
            LK  +   + + P+ A     ++        A K+   S+   K       R    A +
Sbjct: 241 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 300

Query: 301 GRAFEA-SHLEDPLPDNVMVQPLPSVVKIPKGVDEYLELNNSSLRLQKTAEIKQAAKLEP 360
           GRA +A ++L+ P+        LP       G+       +S  ++ K  ++ Q  K + 
Sbjct: 301 GRAIKATTNLDAPMK---KAMDLPRDQGFTNGI-----AVDSEPKISKERDVVQLQKTDG 360

Query: 361 MTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLSEERRLSAQEVKNLKEKLMEL 420
             V+ +++LKLVKDI+EMK K++ +E KL +A  TIS L EER +S+Q  ++L+ +L EL
Sbjct: 361 QNVRGLDELKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAEL 382

Query: 421 SRRDRASNQV--GFPPLFICMVALICIVLGY 436
            R  +   +V  GFP L++C+VA I  V+G+
Sbjct: 421 -RTKKIVKEVHNGFPLLYVCVVAFIAYVIGH 382

BLAST of Tan0020109 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.7e-36
Identity = 70/131 (53.44%), Postives = 97/131 (74.05%), Query Frame = 0

Query: 2   STKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFPK 61
           +++LL ++P +LQF FEL+KQ +C++ L N T ++VAFKVKTT+PKKYCVRPN G++ P+
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKLK 121
           STCE  V MQAQ+ AP DM CKDKFL+Q  I   G T +++T  MFSK+ G  +EE KL+
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VALISPLNSPT 133
           V  ++P   P+
Sbjct: 139 VTYVAPPRPPS 149


HSP 2 Score: 38.1 bits (87), Expect = 3.0e-01
Identity = 20/60 (33.33%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 377 EAQGTISMLSEERRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYV 436
           EA+  I+ L+EE++ + Q    L+ +L +L R  + S   G P +++ +V LI ++LGY+
Sbjct: 193 EARALITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYI 252

BLAST of Tan0020109 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 3.2e-35
Identity = 70/132 (53.03%), Postives = 95/132 (71.97%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MS +LL I P +LQF FEL+KQ +C++ L N T ++VAFKVKTT+PKKYCVRPN G++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           +S+ E  V MQAQ+ AP D+ CKDKFL+Q  +   G T +D+T  MFSK+ G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 KVALISPLNSPT 133
           +V  ++P   P+
Sbjct: 121 RVVYVAPPRPPS 132


HSP 2 Score: 38.5 bits (88), Expect = 2.3e-01
Identity = 19/60 (31.67%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 377 EAQGTISMLSEERRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYV 436
           EA+  ++ L+EE+  + Q    L+++L +L R  + S   G P +++ +V LI ++LGY+
Sbjct: 176 EARALVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYI 235

BLAST of Tan0020109 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 7.8e-34
Identity = 71/132 (53.79%), Postives = 97/132 (73.48%), Query Frame = 0

Query: 5   LLHIQPKELQFIFELRKQSTCTVQLANNTHHH-VAFKVKTTSPKKYCVRPNVGIIFPKST 64
           L++I P EL+F FEL+KQS+C++QL N T    VAFKVKTT+P+KYCVRPN G++ P  +
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 65  CEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKLKVA 124
           C  +V MQAQ+ AP DM CKDKFL+Q+ +V  GTT +++ A MF+K+ G+ IE+ KL+V 
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 125 LISPLNSPTLSP 136
            I P N P+  P
Sbjct: 126 YI-PANPPSPVP 136

BLAST of Tan0020109 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.3e-33
Identity = 86/195 (44.10%), Postives = 120/195 (61.54%), Query Frame = 0

Query: 4   KLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFPKST 63
           +L+ IQP EL+F+FEL KQS C +++AN T ++VAFKVKTTSPKKY VRPN G+I P  +
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 64  CEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKLKVA 123
           C   V +QAQR  PPDM CKDKFL+QSTIVP  T  +++    F+KD GK + E KLKV+
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 124 LISPLNSPTLSPTDGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDAS---DEVL 183
            I+P ++   S   GA               GQ  + ++T +  +LK++ DA+    + L
Sbjct: 128 YITP-STTQRSSESGA-----------TNGDGQSSETISTIQ--RLKEERDAAVKQTQQL 187

Query: 184 SSGVSTLRDQNGPSN 196
              + T+R +    N
Sbjct: 188 QHELETVRRRRNQRN 188

BLAST of Tan0020109 vs. NCBI nr
Match: XP_038890494.1 (vesicle-associated protein 2-2-like [Benincasa hispida] >XP_038890495.1 vesicle-associated protein 2-2-like [Benincasa hispida])

HSP 1 Score: 674.9 bits (1740), Expect = 4.8e-190
Identity = 360/439 (82.00%), Postives = 383/439 (87.24%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQR APPDM CKDKFLIQSTIVPSG TEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRVAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPTDGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEVL 180
           KVALISPLNSP L P+DGA D  MDEV+K N+ SG LRDG+ TNETPKLKDQN ASDEVL
Sbjct: 121 KVALISPLNSPILLPSDGAWDARMDEVMKLNEHSGPLRDGMDTNETPKLKDQNGASDEVL 180

Query: 181 SSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHETS 240
            SGVSTL DQNGPSN+ M HEASRFKDD VA+KG RTIEALKP DQNGA REEVL  E+S
Sbjct: 181 LSGVSTLGDQNGPSNEGMTHEASRFKDDYVALKGERTIEALKPSDQNGAFREEVLPRESS 240

Query: 241 RLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFEASHLEDPL 300
           RLKD NGTSKEE PREAL P  QN ALK EE+S E+PK+RVQNGA E  A+E SHLED +
Sbjct: 241 RLKDPNGTSKEEQPREALKPRDQNRALK-EEMSVESPKKRVQNGALESGAYETSHLEDSV 300

Query: 301 PDNVMVQPLPSVVKIPKGVDEYLELNNSSLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKD 360
           PD + +QP   V+K P+ VDE LELNN   R QKTAEIKQAA+LEP+T+K+ EQLK+VKD
Sbjct: 301 PDTITIQPPSFVLKFPEDVDETLELNNRMSRFQKTAEIKQAAELEPVTLKRAEQLKIVKD 360

Query: 361 IDEMKSKLNEIELKLGEAQGTISMLSEERRLSAQEVKNLKEKLMELSRRDRASNQVGFPP 420
           IDEMKSKLNEIELKLGEAQ TISMLSE RRLSAQEVKNLKEKLMELSRR R SNQVGFPP
Sbjct: 361 IDEMKSKLNEIELKLGEAQVTISMLSEARRLSAQEVKNLKEKLMELSRRGRVSNQVGFPP 420

Query: 421 LFICMVALICIVLGYVFHY 440
           LFICMVALICIV G+V H+
Sbjct: 421 LFICMVALICIVFGFVLHH 438

BLAST of Tan0020109 vs. NCBI nr
Match: KAG6599279.1 (Vesicle-associated protein 2-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 649.8 bits (1675), Expect = 1.7e-182
Identity = 357/472 (75.64%), Postives = 389/472 (82.42%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KST EFSVIMQAQRAAPPDM C+DKFLIQSTI+PSG TEEDITASMF KDGGKYIEEDKL
Sbjct: 61  KSTYEFSVIMQAQRAAPPDMLCRDKFLIQSTIIPSGMTEEDITASMFLKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPT-DGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEV 180
           KVALISPLNSP LSPT DGAL T MDEV+K N+ SG LRDG+ATN+TPKLKDQN +SDEV
Sbjct: 121 KVALISPLNSPLLSPTDDGALGTSMDEVMKLNEHSGPLRDGIATNKTPKLKDQNGSSDEV 180

Query: 181 LSSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHET 240
            SSGVS+LRDQNG SN+ M  EASRFKDD VA+KG RTIEALKP DQNGA R+EVL HET
Sbjct: 181 RSSGVSSLRDQNGQSNEGMTDEASRFKDDFVALKGVRTIEALKPSDQNGALRKEVLPHET 240

Query: 241 SRLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFE------- 300
           SRL DQNGTSKEELP EAL PS QNGALK EELS E PK+RVQNGA + RA+E       
Sbjct: 241 SRLMDQNGTSKEELPHEALKPSDQNGALK-EELSVEVPKKRVQNGALDWRAYETSNVENL 300

Query: 301 -------------------------ASHLEDPLPDNVMVQPLPSVVKIPKGVDEYLELNN 360
                                     SH+ED +P+ V ++P    VK P GVDE LELNN
Sbjct: 301 PPTVVDVQSPRPFERIFPEGGLAYKTSHVEDQVPETVAIRPPDFSVKFPDGVDESLELNN 360

Query: 361 SSLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLSE 420
           S  RLQK+AEIK+AA+L+ +T+KK EQLKLVKDI+EMKSKLNEIELKLGEAQGTISML E
Sbjct: 361 SVSRLQKSAEIKKAAELDLVTLKKAEQLKLVKDIEEMKSKLNEIELKLGEAQGTISMLFE 420

Query: 421 ERRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYVFHY 440
            RRLSAQEVK+LKEKLMELSR+ RASNQVGFPPLFICMVALIC+VLG++ H+
Sbjct: 421 SRRLSAQEVKHLKEKLMELSRKGRASNQVGFPPLFICMVALICVVLGFILHH 471

BLAST of Tan0020109 vs. NCBI nr
Match: XP_023522077.1 (vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023522078.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546957.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546958.1 vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 648.7 bits (1672), Expect = 3.7e-182
Identity = 355/471 (75.37%), Postives = 387/471 (82.17%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KST EFSVIMQAQRAAPPDM C+DKFLIQSTIVPSG TEEDITASMF KDGGKYIEEDKL
Sbjct: 61  KSTYEFSVIMQAQRAAPPDMLCRDKFLIQSTIVPSGMTEEDITASMFLKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPT-DGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEV 180
           KVALISPLNSP LSPT DGAL   MDEV+K N+ SG LRDG+ATN+TPKLKDQN +SDEV
Sbjct: 121 KVALISPLNSPLLSPTDDGALGASMDEVMKLNEHSGPLRDGIATNKTPKLKDQNGSSDEV 180

Query: 181 LSSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHET 240
            SSGVS+LRDQNG SN+ M +EASRFKDD VA+KG RTIEALKP DQNGA R+EVL HET
Sbjct: 181 RSSGVSSLRDQNGQSNEGMTNEASRFKDDFVALKGVRTIEALKPSDQNGALRKEVLPHET 240

Query: 241 SRLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFE------- 300
           SRL DQNGTSKEELP EAL  S QNGA KEEELS E PK+RVQNGA + RA+E       
Sbjct: 241 SRLMDQNGTSKEELPHEALKLSDQNGASKEEELSVEVPKKRVQNGALDWRAYETSNVENL 300

Query: 301 ------------------------ASHLEDPLPDNVMVQPLPSVVKIPKGVDEYLELNNS 360
                                    SH+EDP+P+ V ++P    VK P  VDE LELNN 
Sbjct: 301 PPTVVDVQPPPFERIFPEGGLAYKTSHVEDPVPETVAIRPPDFSVKFPDSVDESLELNNG 360

Query: 361 SLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLSEE 420
             RLQK+AEIK+AA+L+ +T+KK EQLKLVKDI+EMKSKLNEIELKLGEAQGTISML E 
Sbjct: 361 VSRLQKSAEIKKAAELDLVTLKKAEQLKLVKDIEEMKSKLNEIELKLGEAQGTISMLFES 420

Query: 421 RRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYVFHY 440
           RRLSAQEVK+LKEKLMELSR+ RASNQVGFPPLFICMVALIC+VLG++ H+
Sbjct: 421 RRLSAQEVKHLKEKLMELSRKGRASNQVGFPPLFICMVALICVVLGFILHH 471

BLAST of Tan0020109 vs. NCBI nr
Match: XP_022946279.1 (vesicle-associated protein 2-2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 647.9 bits (1670), Expect = 6.3e-182
Identity = 357/472 (75.64%), Postives = 388/472 (82.20%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KST EFSVIMQAQRAAPPDM C+DKFLIQSTIVPSG TEEDITASMF KDGGKYIEEDKL
Sbjct: 61  KSTYEFSVIMQAQRAAPPDMLCRDKFLIQSTIVPSGMTEEDITASMFLKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPT-DGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEV 180
           KVALISPLNSP LSPT DGAL T MDEV+K N+ SG LRDG+ATN+TPKLKDQN +SDEV
Sbjct: 121 KVALISPLNSPLLSPTDDGALGTSMDEVMKLNEHSGPLRDGIATNKTPKLKDQNGSSDEV 180

Query: 181 LSSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHET 240
            SSGVS+LRDQNG SN+ M  EASRFKDD VA+KG RTIEALKP DQNGA R+EVL HET
Sbjct: 181 RSSGVSSLRDQNGQSNEGMTDEASRFKDDFVALKGVRTIEALKPSDQNGALRKEVLPHET 240

Query: 241 SRLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFE------- 300
           SRL DQNGTSKEELP EAL PS QNGALK EELS E PK+RVQNGA + RA+E       
Sbjct: 241 SRLMDQNGTSKEELPHEALKPSDQNGALK-EELSVEVPKKRVQNGALDWRAYETSNVENL 300

Query: 301 -------------------------ASHLEDPLPDNVMVQPLPSVVKIPKGVDEYLELNN 360
                                     SH+ED +P+ V ++P    VK P  VDE LELNN
Sbjct: 301 PPTVVDVQSPRPFERIFPEGGLAYKTSHVEDQVPETVAIRPPDFSVKFPDSVDESLELNN 360

Query: 361 SSLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLSE 420
           S  RLQK+AEIK+AA+L+ +T+KK EQLKLVKDI+EMKSKLNEIELKLGEAQGTISML E
Sbjct: 361 SVSRLQKSAEIKKAAELDLVTLKKAEQLKLVKDIEEMKSKLNEIELKLGEAQGTISMLFE 420

Query: 421 ERRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYVFHY 440
            RRLSAQEVK+LKEKLMELSR+ RASNQVGFPPLFICMVALIC+VLG++ H+
Sbjct: 421 SRRLSAQEVKHLKEKLMELSRKGRASNQVGFPPLFICMVALICVVLGFILHH 471

BLAST of Tan0020109 vs. NCBI nr
Match: XP_022143133.1 (vesicle-associated protein 2-2-like [Momordica charantia] >XP_022143134.1 vesicle-associated protein 2-2-like [Momordica charantia])

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-181
Identity = 351/439 (79.95%), Postives = 377/439 (85.88%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLL+IQPKELQFIFEL+KQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLNIQPKELQFIFELKKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KSTC+FSVIMQAQRAAPPDM CKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCDFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPTDGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEVL 180
           KVALISPLNSP LSPTDG LD IMD +VK + KSG LRDGLATNE PK K QN ASDE+L
Sbjct: 121 KVALISPLNSPILSPTDGELDEIMDGIVKPSGKSGPLRDGLATNEIPKPKYQNGASDEML 180

Query: 181 SSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHETS 240
            SGVSTLR    PSN+ M HEASR KDD VA KGG+ IEA KP DQNG  REEVLLH+TS
Sbjct: 181 PSGVSTLR----PSNEGMTHEASRLKDDFVASKGGQPIEASKPSDQNGELREEVLLHQTS 240

Query: 241 RLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFEASHLEDPL 300
           RLKDQNG SKEE+PREAL P  QNG+ KEEEL+ EAPK+RVQNGA EG+ +E SHL+DPL
Sbjct: 241 RLKDQNGISKEEIPREALKPRDQNGSSKEEELTLEAPKKRVQNGALEGQGYETSHLKDPL 300

Query: 301 PDNVMVQPLPSVVKIPKGVDEYLELNNSSLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKD 360
               ++QP P  V    GVD+  ELNNS+ RLQKTAEIKQAA+ E MTVKK ++LKLVKD
Sbjct: 301 SSGSVIQP-PEYV---AGVDKSQELNNSTSRLQKTAEIKQAAESELMTVKKSDRLKLVKD 360

Query: 361 IDEMKSKLNEIELKLGEAQGTISMLSEERRLSAQEVKNLKEKLMELSRRDRASNQVGFPP 420
           IDEMKSKLNEIELKLGEAQGTISMLS ERR SAQEVK LK+K+MELSRR R  NQVGFPP
Sbjct: 361 IDEMKSKLNEIELKLGEAQGTISMLSAERRSSAQEVKILKDKMMELSRRGRVRNQVGFPP 420

Query: 421 LFICMVALICIVLGYVFHY 440
           LFICMV LICIVLGYV H+
Sbjct: 421 LFICMVTLICIVLGYVLHH 431

BLAST of Tan0020109 vs. ExPASy TrEMBL
Match: A0A6J1G3C7 (vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450409 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 3.1e-182
Identity = 357/472 (75.64%), Postives = 388/472 (82.20%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KST EFSVIMQAQRAAPPDM C+DKFLIQSTIVPSG TEEDITASMF KDGGKYIEEDKL
Sbjct: 61  KSTYEFSVIMQAQRAAPPDMLCRDKFLIQSTIVPSGMTEEDITASMFLKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPT-DGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEV 180
           KVALISPLNSP LSPT DGAL T MDEV+K N+ SG LRDG+ATN+TPKLKDQN +SDEV
Sbjct: 121 KVALISPLNSPLLSPTDDGALGTSMDEVMKLNEHSGPLRDGIATNKTPKLKDQNGSSDEV 180

Query: 181 LSSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHET 240
            SSGVS+LRDQNG SN+ M  EASRFKDD VA+KG RTIEALKP DQNGA R+EVL HET
Sbjct: 181 RSSGVSSLRDQNGQSNEGMTDEASRFKDDFVALKGVRTIEALKPSDQNGALRKEVLPHET 240

Query: 241 SRLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFE------- 300
           SRL DQNGTSKEELP EAL PS QNGALK EELS E PK+RVQNGA + RA+E       
Sbjct: 241 SRLMDQNGTSKEELPHEALKPSDQNGALK-EELSVEVPKKRVQNGALDWRAYETSNVENL 300

Query: 301 -------------------------ASHLEDPLPDNVMVQPLPSVVKIPKGVDEYLELNN 360
                                     SH+ED +P+ V ++P    VK P  VDE LELNN
Sbjct: 301 PPTVVDVQSPRPFERIFPEGGLAYKTSHVEDQVPETVAIRPPDFSVKFPDSVDESLELNN 360

Query: 361 SSLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLSE 420
           S  RLQK+AEIK+AA+L+ +T+KK EQLKLVKDI+EMKSKLNEIELKLGEAQGTISML E
Sbjct: 361 SVSRLQKSAEIKKAAELDLVTLKKAEQLKLVKDIEEMKSKLNEIELKLGEAQGTISMLFE 420

Query: 421 ERRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYVFHY 440
            RRLSAQEVK+LKEKLMELSR+ RASNQVGFPPLFICMVALIC+VLG++ H+
Sbjct: 421 SRRLSAQEVKHLKEKLMELSRKGRASNQVGFPPLFICMVALICVVLGFILHH 471

BLAST of Tan0020109 vs. ExPASy TrEMBL
Match: A0A6J1CPY8 (vesicle-associated protein 2-2-like OS=Momordica charantia OX=3673 GN=LOC111013073 PE=4 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 5.2e-182
Identity = 351/439 (79.95%), Postives = 377/439 (85.88%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLL+IQPKELQFIFEL+KQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLNIQPKELQFIFELKKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KSTC+FSVIMQAQRAAPPDM CKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCDFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPTDGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEVL 180
           KVALISPLNSP LSPTDG LD IMD +VK + KSG LRDGLATNE PK K QN ASDE+L
Sbjct: 121 KVALISPLNSPILSPTDGELDEIMDGIVKPSGKSGPLRDGLATNEIPKPKYQNGASDEML 180

Query: 181 SSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHETS 240
            SGVSTLR    PSN+ M HEASR KDD VA KGG+ IEA KP DQNG  REEVLLH+TS
Sbjct: 181 PSGVSTLR----PSNEGMTHEASRLKDDFVASKGGQPIEASKPSDQNGELREEVLLHQTS 240

Query: 241 RLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFEASHLEDPL 300
           RLKDQNG SKEE+PREAL P  QNG+ KEEEL+ EAPK+RVQNGA EG+ +E SHL+DPL
Sbjct: 241 RLKDQNGISKEEIPREALKPRDQNGSSKEEELTLEAPKKRVQNGALEGQGYETSHLKDPL 300

Query: 301 PDNVMVQPLPSVVKIPKGVDEYLELNNSSLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKD 360
               ++QP P  V    GVD+  ELNNS+ RLQKTAEIKQAA+ E MTVKK ++LKLVKD
Sbjct: 301 SSGSVIQP-PEYV---AGVDKSQELNNSTSRLQKTAEIKQAAESELMTVKKSDRLKLVKD 360

Query: 361 IDEMKSKLNEIELKLGEAQGTISMLSEERRLSAQEVKNLKEKLMELSRRDRASNQVGFPP 420
           IDEMKSKLNEIELKLGEAQGTISMLS ERR SAQEVK LK+K+MELSRR R  NQVGFPP
Sbjct: 361 IDEMKSKLNEIELKLGEAQGTISMLSAERRSSAQEVKILKDKMMELSRRGRVRNQVGFPP 420

Query: 421 LFICMVALICIVLGYVFHY 440
           LFICMV LICIVLGYV H+
Sbjct: 421 LFICMVTLICIVLGYVLHH 431

BLAST of Tan0020109 vs. ExPASy TrEMBL
Match: A0A0A0LIH5 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013300 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 6.8e-182
Identity = 349/439 (79.50%), Postives = 375/439 (85.42%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLLHIQPKELQF+FELRKQSTCTVQL+NNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLHIQPKELQFLFELRKQSTCTVQLSNNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KSTCEFSVIMQAQRAAPPDM CKDKFLIQSTIVPSG TEEDITASMFSKDGGKYIEEDKL
Sbjct: 61  KSTCEFSVIMQAQRAAPPDMLCKDKFLIQSTIVPSGITEEDITASMFSKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPTDGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEVL 180
           KVALISPLNSP LSP+DGALD  MD+V+K N+ SG L DG+ TNETPKLKDQN ASDEVL
Sbjct: 121 KVALISPLNSPILSPSDGALDARMDDVMKLNEDSGPLSDGMDTNETPKLKDQNGASDEVL 180

Query: 181 SSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHETS 240
            S V  LR QNG SN+ M HEASRFKDD VA+KG RTIEALKP DQNGA REEVL HE S
Sbjct: 181 PSVVPILRYQNGLSNEGMTHEASRFKDDFVALKGERTIEALKPSDQNGAFREEVLPHEFS 240

Query: 241 RLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFEASHLEDPL 300
           RLKD N TSKEELPREAL P  QN ALKEEELS E+P ++VQNGA +  A+ +SHLE+P+
Sbjct: 241 RLKDPNETSKEELPREALKPRDQNRALKEEELSVESPTKKVQNGALKWGAYGSSHLENPV 300

Query: 301 PDNVMVQPLPSVVKIPKGVDEYLELNNSSLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKD 360
           PD           K P+  DE LELNNS  RLQKTAEIKQ  +LEP+ +KK EQL+LVKD
Sbjct: 301 PD----------TKFPEDADESLELNNSMSRLQKTAEIKQGTELEPLKLKKAEQLQLVKD 360

Query: 361 IDEMKSKLNEIELKLGEAQGTISMLSEERRLSAQEVKNLKEKLMELSRRDRASNQVGFPP 420
           IDEMKSKL+EIELKLGEAQGTISMLSE RRLSAQEVK LKEKL+ELSRR RASNQVGFPP
Sbjct: 361 IDEMKSKLHEIELKLGEAQGTISMLSEARRLSAQEVKILKEKLLELSRRGRASNQVGFPP 420

Query: 421 LFICMVALICIVLGYVFHY 440
           LFICMVALICIV G+V H+
Sbjct: 421 LFICMVALICIVFGFVLHH 429

BLAST of Tan0020109 vs. ExPASy TrEMBL
Match: A0A6J1KF63 (vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493755 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 3.4e-181
Identity = 354/471 (75.16%), Postives = 387/471 (82.17%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KST EFSVIMQAQRAAPPDM C+DKFLIQSTIVPSG TEEDITASMF KDGGKYIEEDKL
Sbjct: 61  KSTYEFSVIMQAQRAAPPDMLCRDKFLIQSTIVPSGMTEEDITASMFLKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPT-DGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEV 180
           KVALISPLNSP LSPT DGAL   MDEV+K N+ SG LRDG+ATN+TPKLKDQN +SDEV
Sbjct: 121 KVALISPLNSPLLSPTDDGALGASMDEVMKLNEHSGPLRDGIATNKTPKLKDQNGSSDEV 180

Query: 181 LSSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHET 240
            SSGVS+LRDQNG SN+ M  EASRFKDD VA+KG RTIEALKP D+NGA R+EVL HET
Sbjct: 181 RSSGVSSLRDQNGQSNEGMTDEASRFKDDFVALKGVRTIEALKPSDKNGALRKEVLPHET 240

Query: 241 SRLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFE------- 300
           SRL DQNGTSKEELP EAL  S QNGALKEEELS E PK+RVQNGA + RA+E       
Sbjct: 241 SRLMDQNGTSKEELPHEALKLSDQNGALKEEELSVEVPKKRVQNGALDWRAYETSNVENL 300

Query: 301 ------------------------ASHLEDPLPDNVMVQPLPSVVKIPKGVDEYLELNNS 360
                                   ASH+ED +P+ V ++P    +K P  VDE LELNNS
Sbjct: 301 PPTVVDVQPPPFERIFPEGGLAYKASHVEDLVPETVAIRPPDFSLKFPDSVDESLELNNS 360

Query: 361 SLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLSEE 420
              LQK+AEI++AA+L+ +T+KK EQLKLVKDIDEMKSKLNEIELKLGEAQGTISML E 
Sbjct: 361 VSGLQKSAEIRKAAELDLVTLKKAEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLFES 420

Query: 421 RRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYVFHY 440
           RRLSAQEVK+LKEKLMELSR+ RASNQVGFPPLFICMVALIC+VLG++ H+
Sbjct: 421 RRLSAQEVKHLKEKLMELSRKGRASNQVGFPPLFICMVALICVVLGFILHH 471

BLAST of Tan0020109 vs. ExPASy TrEMBL
Match: A0A6J1G3A5 (vesicle-associated protein 2-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450409 PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 4.4e-181
Identity = 357/473 (75.48%), Postives = 389/473 (82.24%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGII P
Sbjct: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIILP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KST EFSVIMQAQRAAPPDM C+DKFLIQSTIVPSG TEEDITASMF KDGGKYIEEDKL
Sbjct: 61  KSTYEFSVIMQAQRAAPPDMLCRDKFLIQSTIVPSGMTEEDITASMFLKDGGKYIEEDKL 120

Query: 121 KVALISPLNSPTLSPT-DGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEV 180
           KVALISPLNSP LSPT DGAL T MDEV+K N+ SG LRDG+ATN+TPKLKDQN +SDEV
Sbjct: 121 KVALISPLNSPLLSPTDDGALGTSMDEVMKLNEHSGPLRDGIATNKTPKLKDQNGSSDEV 180

Query: 181 LSSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHET 240
            SSGVS+LRDQNG SN+ M  EASRFKDD VA+KG RTIEALKP DQNGA R+EVL HET
Sbjct: 181 RSSGVSSLRDQNGQSNEGMTDEASRFKDDFVALKGVRTIEALKPSDQNGALRKEVLPHET 240

Query: 241 SRLKDQNGTSKEELPREALTPSAQNGALKEEELSAEAPKRRVQNGAQEGRAFE------- 300
           SRL DQNGTSKEELP EAL PS QNGALK EELS E PK+RVQNGA + RA+E       
Sbjct: 241 SRLMDQNGTSKEELPHEALKPSDQNGALK-EELSVEVPKKRVQNGALDWRAYETSNVENL 300

Query: 301 -------------------------ASHLEDPLPDNVMVQPLP-SVVKIPKGVDEYLELN 360
                                     SH+ED +P+ V ++P   SV + P  VDE LELN
Sbjct: 301 PPTVVDVQSPRPFERIFPEGGLAYKTSHVEDQVPETVAIRPPDFSVKQFPDSVDESLELN 360

Query: 361 NSSLRLQKTAEIKQAAKLEPMTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLS 420
           NS  RLQK+AEIK+AA+L+ +T+KK EQLKLVKDI+EMKSKLNEIELKLGEAQGTISML 
Sbjct: 361 NSVSRLQKSAEIKKAAELDLVTLKKAEQLKLVKDIEEMKSKLNEIELKLGEAQGTISMLF 420

Query: 421 EERRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYVFHY 440
           E RRLSAQEVK+LKEKLMELSR+ RASNQVGFPPLFICMVALIC+VLG++ H+
Sbjct: 421 ESRRLSAQEVKHLKEKLMELSRKGRASNQVGFPPLFICMVALICVVLGFILHH 472

BLAST of Tan0020109 vs. TAIR 10
Match: AT1G08820.1 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 233.8 bits (595), Expect = 2.6e-61
Identity = 169/451 (37.47%), Postives = 238/451 (52.77%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           M+  LL IQP+ LQF  +L+KQ++C VQL N THH+VAFKVKTTSPKKYCVRPNVG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KSTCEF+VIMQA +  PPDM CKDKFLIQST V + TT+EDITASMFSK  GK+IEE+KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 KVALISPLNSPTLSPTDGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEVL 180
           +V L+ P +SP LSP +                            TPK            
Sbjct: 121 RVTLVPPSDSPELSPIN----------------------------TPK------------ 180

Query: 181 SSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHETS 240
                               + + F+D I+  +     E L P    G   +E  +    
Sbjct: 181 --------------------QGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 240

Query: 241 RLKDQNGTSKEELPREALTPSAQN-------GALKEEELSAEAPKR------RVQNGAQE 300
            LK  +   + + P+ A     ++        A K+   S+   K       R    A +
Sbjct: 241 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 300

Query: 301 GRAFEA-SHLEDPLPDNVMVQPLPSVVKIPKGVDEYLELNNSSLRLQKTAEIKQAAKLEP 360
           GRA +A ++L+ P+        LP       G+       +S  ++ K  ++ Q  K + 
Sbjct: 301 GRAIKATTNLDAPMK---KAMDLPRDQGFTNGI-----AVDSEPKISKERDVVQLQKTDG 360

Query: 361 MTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLSEERRLSAQEVKNLKEKLMEL 420
             V+ +++LKLVKDI+EMK K++ +E KL +A  TIS L EER +S+Q  ++L+ +L EL
Sbjct: 361 QNVRGLDELKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAEL 382

Query: 421 SRRDRASNQV--GFPPLFICMVALICIVLGY 436
            R  +   +V  GFP L++C+VA I  V+G+
Sbjct: 421 -RTKKIVKEVHNGFPLLYVCVVAFIAYVIGH 382

BLAST of Tan0020109 vs. TAIR 10
Match: AT1G08820.2 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 233.8 bits (595), Expect = 2.6e-61
Identity = 169/451 (37.47%), Postives = 238/451 (52.77%), Query Frame = 0

Query: 1   MSTKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFP 60
           M+  LL IQP+ LQF  +L+KQ++C VQL N THH+VAFKVKTTSPKKYCVRPNVG++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KSTCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKL 120
           KSTCEF+VIMQA +  PPDM CKDKFLIQST V + TT+EDITASMFSK  GK+IEE+KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 KVALISPLNSPTLSPTDGALDTIMDEVVKQNKKSGQLRDGLATNETPKLKDQNDASDEVL 180
           +V L+ P +SP LSP +                            TPK            
Sbjct: 121 RVTLVPPSDSPELSPIN----------------------------TPK------------ 180

Query: 181 SSGVSTLRDQNGPSNDSMAHEASRFKDDIVAVKGGRTIEALKPYDQNGASREEVLLHETS 240
                               + + F+D I+  +     E L P    G   +E  +    
Sbjct: 181 --------------------QGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGHD 240

Query: 241 RLKDQNGTSKEELPREALTPSAQN-------GALKEEELSAEAPKR------RVQNGAQE 300
            LK  +   + + P+ A     ++        A K+   S+   K       R    A +
Sbjct: 241 ELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDADD 300

Query: 301 GRAFEA-SHLEDPLPDNVMVQPLPSVVKIPKGVDEYLELNNSSLRLQKTAEIKQAAKLEP 360
           GRA +A ++L+ P+        LP       G+       +S  ++ K  ++ Q  K + 
Sbjct: 301 GRAIKATTNLDAPMK---KAMDLPRDQGFTNGI-----AVDSEPKISKERDVVQLQKTDG 360

Query: 361 MTVKKVEQLKLVKDIDEMKSKLNEIELKLGEAQGTISMLSEERRLSAQEVKNLKEKLMEL 420
             V+ +++LKLVKDI+EMK K++ +E KL +A  TIS L EER +S+Q  ++L+ +L EL
Sbjct: 361 QNVRGLDELKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAEL 382

Query: 421 SRRDRASNQV--GFPPLFICMVALICIVLGY 436
            R  +   +V  GFP L++C+VA I  V+G+
Sbjct: 421 -RTKKIVKEVHNGFPLLYVCVVAFIAYVIGH 382

BLAST of Tan0020109 vs. TAIR 10
Match: AT3G60600.1 (vesicle associated protein )

HSP 1 Score: 155.2 bits (391), Expect = 1.2e-37
Identity = 70/131 (53.44%), Postives = 97/131 (74.05%), Query Frame = 0

Query: 2   STKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFPK 61
           +++LL ++P +LQF FEL+KQ +C++ L N T ++VAFKVKTT+PKKYCVRPN G++ P+
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKLK 121
           STCE  V MQAQ+ AP DM CKDKFL+Q  I   G T +++T  MFSK+ G  +EE KL+
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VALISPLNSPT 133
           V  ++P   P+
Sbjct: 139 VTYVAPPRPPS 149


HSP 2 Score: 38.1 bits (87), Expect = 2.1e-02
Identity = 20/60 (33.33%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 377 EAQGTISMLSEERRLSAQEVKNLKEKLMELSRRDRASNQVGFPPLFICMVALICIVLGYV 436
           EA+  I+ L+EE++ + Q    L+ +L +L R  + S   G P +++ +V LI ++LGY+
Sbjct: 193 EARALITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYI 252

BLAST of Tan0020109 vs. TAIR 10
Match: AT3G60600.2 (vesicle associated protein )

HSP 1 Score: 155.2 bits (391), Expect = 1.2e-37
Identity = 70/131 (53.44%), Postives = 97/131 (74.05%), Query Frame = 0

Query: 2   STKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFPK 61
           +++LL ++P +LQF FEL+KQ +C++ L N T ++VAFKVKTT+PKKYCVRPN G++ P+
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKLK 121
           STCE  V MQAQ+ AP DM CKDKFL+Q  I   G T +++T  MFSK+ G  +EE KL+
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VALISPLNSPT 133
           V  ++P   P+
Sbjct: 139 VTYVAPPRPPS 149

BLAST of Tan0020109 vs. TAIR 10
Match: AT3G60600.3 (vesicle associated protein )

HSP 1 Score: 155.2 bits (391), Expect = 1.2e-37
Identity = 70/131 (53.44%), Postives = 97/131 (74.05%), Query Frame = 0

Query: 2   STKLLHIQPKELQFIFELRKQSTCTVQLANNTHHHVAFKVKTTSPKKYCVRPNVGIIFPK 61
           +++LL ++P +LQF FEL+KQ +C++ L N T ++VAFKVKTT+PKKYCVRPN G++ P+
Sbjct: 19  NSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPR 78

Query: 62  STCEFSVIMQAQRAAPPDMFCKDKFLIQSTIVPSGTTEEDITASMFSKDGGKYIEEDKLK 121
           STCE  V MQAQ+ AP DM CKDKFL+Q  I   G T +++T  MFSK+ G  +EE KL+
Sbjct: 79  STCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLR 138

Query: 122 VALISPLNSPT 133
           V  ++P   P+
Sbjct: 139 VTYVAPPRPPS 149

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9DHD73.7e-6037.47Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Q8VZ951.7e-3653.44Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q9SHC83.2e-3553.03Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q84WW57.8e-3453.79Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q9LVU12.3e-3344.10Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_038890494.14.8e-19082.00vesicle-associated protein 2-2-like [Benincasa hispida] >XP_038890495.1 vesicle-... [more]
KAG6599279.11.7e-18275.64Vesicle-associated protein 2-2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023522077.13.7e-18275.37vesicle-associated protein 2-2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_... [more]
XP_022946279.16.3e-18275.64vesicle-associated protein 2-2-like isoform X2 [Cucurbita moschata][more]
XP_022143133.11.1e-18179.95vesicle-associated protein 2-2-like [Momordica charantia] >XP_022143134.1 vesicl... [more]
Match NameE-valueIdentityDescription
A0A6J1G3C73.1e-18275.64vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1CPY85.2e-18279.95vesicle-associated protein 2-2-like OS=Momordica charantia OX=3673 GN=LOC1110130... [more]
A0A0A0LIH56.8e-18279.50MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013300 PE=4 SV... [more]
A0A6J1KF633.4e-18175.16vesicle-associated protein 2-2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1G3A54.4e-18175.48vesicle-associated protein 2-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
AT1G08820.12.6e-6137.47vamp/synaptobrevin-associated protein 27-2 [more]
AT1G08820.22.6e-6137.47vamp/synaptobrevin-associated protein 27-2 [more]
AT3G60600.11.2e-3753.44vesicle associated protein [more]
AT3G60600.21.2e-3753.44vesicle associated protein [more]
AT3G60600.31.2e-3753.44vesicle associated protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 361..402
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..283
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 176..198
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 151..202
NoneNo IPR availablePANTHERPTHR10809:SF45VESICLE-ASSOCIATED PROTEIN 2-2coord: 1..404
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 6..111
e-value: 1.3E-30
score: 105.4
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 5..125
score: 29.137711
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 1..124
e-value: 6.2E-42
score: 144.4
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..404
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 4..134

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0020109.1Tan0020109.1mRNA
Tan0020109.2Tan0020109.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane