Tan0019871 (gene) Snake gourd v1

Overview
NameTan0019871
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionProtein NRT1/ PTR FAMILY 6.1
LocationLG10: 20615614 .. 20618546 (+)
RNA-Seq ExpressionTan0019871
SyntenyTan0019871
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGGTTGGATGTGTATTAATTAGGCAGTCAAAATCACTCACTAACTGTTAAGAAACTTACAGAAAATTATAACCTTATAACCAAAGATATCTCTTTGCAACTGCAGATGGTACTGGGTTTGACAAATCTCTGCAGCAGGTTTGGAGAACAATTTTGTTTTGAATTCTTAGTGAAAATAGTGAGTGGAAGTGTTTGTATGAACATAGTGTTTGGTTTTTCTGTTTGTGTTTGTGTGTGTGTTTTTTTTCTTCTTTTTCCCAGGAATTTTTGAAGTATGGGAAGTAGGGAAATCAAATCACCTCAGGAGGTGATTGAAATGCCTGTAAGATTGGATGAGCATTCAGAGTCATTTCAGAGGAAGAAGCTTGGGATGTTTTTCATCGAATCGGATGATCGGAGGACGGCGTTTGGGCGTGGTTACACTGGAGGAACGACTCCGGTTAATATCCGTGGTAAACCGATTGCTGATCTTTCAAAGACTGGGGGCTGGCTTGCAGCCTTCTTCATTTTTGGTAAGCAGCTGCAAAACAACCTTGTTGTTTGTTGTTTGTTGTTACTATTTGAACTTGAGAAAGTAAAGAGGAAAATTTTAAAAATTCACTCTGTTTTGTTTACAAGGGAATGAAATGGCAGAGAGAATGGCTTATTTTGGGCTTTCAGTGAACATGGTAGCCTTTATGTTCTATGTGATGCATATGCCATTTACTACTTCATCAGATGCAGTGAACAATTTCCTTGGGATCTCACAAGCATCCTCTGTTCTTGGCGGTTTCCTTGCCGATGCATATCTCGGTCGATATTGGACGATTGCGATCTTTACTACCATCTATCTCGGGGTACGATACGATGTTATAAAACCCTATCAGTACAAAGCTGATGAGGGCCAAATATTGAATTGAAATCCTTGGTTTGTACTTAATGCAGGGTTTAACAGGAATAACTCTGTGTGCCACAATTAGTGCATTTGTGCCAAACCAAGAGAAATGTGATCAGTTATCACTGCTTCTAGGCAAATGTGAGCCAGCAAAGTCATGGCAAATGACTTACCTTTACACAGTCCTTTATTTGACAGGGTTTGGGGCTGCAGGTATAAGGCCATGTGTCTCTTCTTTTGGGGCTGATCAGTTTGATGAGAAAAGCAAAGACTACAAATCTCACTTGGATCGTTTTTTCAACTTCTTTTACCTTTCTGTGACAATTGGAGCCATTGTGGCCTTCACTGCTGTTGTTTACATTCAAATTAAGCATGGATGGGGGGCTGCTTTTGGCTCCCTAGCCATCGCCATGGGCTTCTCGAACGTCGTCTTTTTTATCGGTACTCCTTTGTACCGGCATCGGTTGCCAGGAGGTAGCCCTCTCACAAGGGTTGCCCAAGTTCTTGTGGCTGCATTCAGGAAGAGAAAGACTTCATTTTCTAGCAGCGAGATGGTTGGCTTGTACGAGGTTCCCGGCAAGCATTCCGCTATCAAAGGCAGCGGAAAGATCCTTCACACCAATGATTTCAGGTATGTGGTATTCGAGTAAGAGATCTTTATATGGTAGATTTCTACGTCAAGTCAACTTACCACATTTTATCCATGTAAGCTTTTGAATTTAGTGGTGATTTGGCCGTCATATGCACGGGTTGCAGATGTTTGGACAAGGCAGCACTACAGTTAAAGGAAGATGGAGGCAATCCAAGTCCATGGAGGCTTTGCACAGTGACTCAAGTTGAGGAAGTGAAGATTCTTTTGAAGCTGATTCCAATACCAGCCTGCACAATCATGCTCAATTTAGTGCTAACAGAATATCTCACTCTTTCAGTGCAACAAGCATACACTTTGAACACTCACATGGGCCGTCTTAAGCTCCCTGTCACATGCATGCCAGTCTTCCCTGGCCTCAGCATATTCCTCATACTTTCTCTCTACTACTCAGTATTCGTTCCGCTCTCTCGACGCATCACCGGCCACCCGCACGGTGCTTCTCAGCTACAAAGAGTCGGCATTGGTCTAGCAATCTCGATACTCTCGGTCGCTTGGGCTGGGGCATTCGAGCGATACAGAAGGAACTTTGCTATCAAAAATGGATATGAGGCCAGTTTTTTGACTCCTATGCCCAACTTGACTGCCTACTGGCTGCTCATTCAGTATTGTCTCATTGGCATAGCAGAAGTGTTTTGCATTGTTGGTTTGCTTGAGTTCTTATATGAGGAAGCTCCGGACGCCATGAAGAGCATTGGATCGGCATACGCCGCTCTCGCAGGCGGCTTGGGTTGCTTCGCAGCATCGTTGTTGAACAGTATTATCAAATCCGTCACGGGGAACCCGAACGAAAGAATTCCGTCCTGGCTTTCTCAAAATATTAACACGGGACGATTCGACTACTTCTACTGGCTTCTCACAGTTATGAGTATCATCAATTTCTGCATCTTTCTGTACTCAGCTCATAGGTACAAATACAGGAAAGATCATGAAGTTGGGGAAGGAATCATGGAAAATGGAAGGCATGATAAAATGTGAGATCGATTCATTCACTTTTCTTTTATATTTTGACAGAGTGAAGTACCATAATTGGTTAGTATTAGAGTTGTTTTTTAGAAATGTAAAAAAGAAATCAAAGCTACTTCTTCTACTAGGAGTATTGGATTTCATAGTTGGAAAAAGTTAGAGAAATTATTGAATGAAGCAACCACACACTTTCAAAACGGTTAAAAAGTATTTTTTAGGTTAAATCACTTTTGGTCCCTAAAACTTTTTATTGTAGTAACAGTTTAGTCTTTGTAACGATTTAGTCATGTACTTTCAATTTGTAACAATTTAGTTCATGAGCTTTGGTATGTAACAATTTAGTTCATATACTTTAAAATTTGTAATGATATTTGTTAACATAGAAAAGCAGATGACTAACGACGAATGCAGAAGAAAAATGACACACAGATTTACGTGATTCA

mRNA sequence

TTTGGTTGGATGTGTATTAATTAGGCAGTCAAAATCACTCACTAACTGTTAAGAAACTTACAGAAAATTATAACCTTATAACCAAAGATATCTCTTTGCAACTGCAGATGGTACTGGGTTTGACAAATCTCTGCAGCAGGTTTGGAGAACAATTTTGTTTTGAATTCTTAGTGAAAATAGTGAGTGGAAGTGTTTGTATGAACATAGTGTTTGGTTTTTCTGTTTGTGTTTGTGTGTGTGTTTTTTTTCTTCTTTTTCCCAGGAATTTTTGAAGTATGGGAAGTAGGGAAATCAAATCACCTCAGGAGGTGATTGAAATGCCTGTAAGATTGGATGAGCATTCAGAGTCATTTCAGAGGAAGAAGCTTGGGATGTTTTTCATCGAATCGGATGATCGGAGGACGGCGTTTGGGCGTGGTTACACTGGAGGAACGACTCCGGTTAATATCCGTGGTAAACCGATTGCTGATCTTTCAAAGACTGGGGGCTGGCTTGCAGCCTTCTTCATTTTTGGGAATGAAATGGCAGAGAGAATGGCTTATTTTGGGCTTTCAGTGAACATGGTAGCCTTTATGTTCTATGTGATGCATATGCCATTTACTACTTCATCAGATGCAGTGAACAATTTCCTTGGGATCTCACAAGCATCCTCTGTTCTTGGCGGTTTCCTTGCCGATGCATATCTCGGTCGATATTGGACGATTGCGATCTTTACTACCATCTATCTCGGGGGTTTAACAGGAATAACTCTGTGTGCCACAATTAGTGCATTTGTGCCAAACCAAGAGAAATGTGATCAGTTATCACTGCTTCTAGGCAAATGTGAGCCAGCAAAGTCATGGCAAATGACTTACCTTTACACAGTCCTTTATTTGACAGGGTTTGGGGCTGCAGGTATAAGGCCATGTGTCTCTTCTTTTGGGGCTGATCAGTTTGATGAGAAAAGCAAAGACTACAAATCTCACTTGGATCGTTTTTTCAACTTCTTTTACCTTTCTGTGACAATTGGAGCCATTGTGGCCTTCACTGCTGTTGTTTACATTCAAATTAAGCATGGATGGGGGGCTGCTTTTGGCTCCCTAGCCATCGCCATGGGCTTCTCGAACGTCGTCTTTTTTATCGGTACTCCTTTGTACCGGCATCGGTTGCCAGGAGGTAGCCCTCTCACAAGGGTTGCCCAAGTTCTTGTGGCTGCATTCAGGAAGAGAAAGACTTCATTTTCTAGCAGCGAGATGGTTGGCTTGTACGAGGTTCCCGGCAAGCATTCCGCTATCAAAGGCAGCGGAAAGATCCTTCACACCAATGATTTCAGATGTTTGGACAAGGCAGCACTACAGTTAAAGGAAGATGGAGGCAATCCAAGTCCATGGAGGCTTTGCACAGTGACTCAAGTTGAGGAAGTGAAGATTCTTTTGAAGCTGATTCCAATACCAGCCTGCACAATCATGCTCAATTTAGTGCTAACAGAATATCTCACTCTTTCAGTGCAACAAGCATACACTTTGAACACTCACATGGGCCGTCTTAAGCTCCCTGTCACATGCATGCCAGTCTTCCCTGGCCTCAGCATATTCCTCATACTTTCTCTCTACTACTCAGTATTCGTTCCGCTCTCTCGACGCATCACCGGCCACCCGCACGGTGCTTCTCAGCTACAAAGAGTCGGCATTGGTCTAGCAATCTCGATACTCTCGGTCGCTTGGGCTGGGGCATTCGAGCGATACAGAAGGAACTTTGCTATCAAAAATGGATATGAGGCCAGTTTTTTGACTCCTATGCCCAACTTGACTGCCTACTGGCTGCTCATTCAGTATTGTCTCATTGGCATAGCAGAAGTGTTTTGCATTGTTGGTTTGCTTGAGTTCTTATATGAGGAAGCTCCGGACGCCATGAAGAGCATTGGATCGGCATACGCCGCTCTCGCAGGCGGCTTGGGTTGCTTCGCAGCATCGTTGTTGAACAGTATTATCAAATCCGTCACGGGGAACCCGAACGAAAGAATTCCGTCCTGGCTTTCTCAAAATATTAACACGGGACGATTCGACTACTTCTACTGGCTTCTCACAGTTATGAGTATCATCAATTTCTGCATCTTTCTGTACTCAGCTCATAGGTACAAATACAGGAAAGATCATGAAGTTGGGGAAGGAATCATGGAAAATGGAAGGCATGATAAAATGTGAGATCGATTCATTCACTTTTCTTTTATATTTTGACAGAGTGAAGTACCATAATTGGTTAGTATTAGAGTTGTTTTTTAGAAATGTAAAAAAGAAATCAAAGCTACTTCTTCTACTAGGAGTATTGGATTTCATAGTTGGAAAAAGTTAGAGAAATTATTGAATGAAGCAACCACACACTTTCAAAACGGTTAAAAAGTATTTTTTAGGTTAAATCACTTTTGGTCCCTAAAACTTTTTATTGTAGTAACAGTTTAGTCTTTGTAACGATTTAGTCATGTACTTTCAATTTGTAACAATTTAGTTCATGAGCTTTGGTATGTAACAATTTAGTTCATATACTTTAAAATTTGTAATGATATTTGTTAACATAGAAAAGCAGATGACTAACGACGAATGCAGAAGAAAAATGACACACAGATTTACGTGATTCA

Coding sequence (CDS)

ATGGGAAGTAGGGAAATCAAATCACCTCAGGAGGTGATTGAAATGCCTGTAAGATTGGATGAGCATTCAGAGTCATTTCAGAGGAAGAAGCTTGGGATGTTTTTCATCGAATCGGATGATCGGAGGACGGCGTTTGGGCGTGGTTACACTGGAGGAACGACTCCGGTTAATATCCGTGGTAAACCGATTGCTGATCTTTCAAAGACTGGGGGCTGGCTTGCAGCCTTCTTCATTTTTGGGAATGAAATGGCAGAGAGAATGGCTTATTTTGGGCTTTCAGTGAACATGGTAGCCTTTATGTTCTATGTGATGCATATGCCATTTACTACTTCATCAGATGCAGTGAACAATTTCCTTGGGATCTCACAAGCATCCTCTGTTCTTGGCGGTTTCCTTGCCGATGCATATCTCGGTCGATATTGGACGATTGCGATCTTTACTACCATCTATCTCGGGGGTTTAACAGGAATAACTCTGTGTGCCACAATTAGTGCATTTGTGCCAAACCAAGAGAAATGTGATCAGTTATCACTGCTTCTAGGCAAATGTGAGCCAGCAAAGTCATGGCAAATGACTTACCTTTACACAGTCCTTTATTTGACAGGGTTTGGGGCTGCAGGTATAAGGCCATGTGTCTCTTCTTTTGGGGCTGATCAGTTTGATGAGAAAAGCAAAGACTACAAATCTCACTTGGATCGTTTTTTCAACTTCTTTTACCTTTCTGTGACAATTGGAGCCATTGTGGCCTTCACTGCTGTTGTTTACATTCAAATTAAGCATGGATGGGGGGCTGCTTTTGGCTCCCTAGCCATCGCCATGGGCTTCTCGAACGTCGTCTTTTTTATCGGTACTCCTTTGTACCGGCATCGGTTGCCAGGAGGTAGCCCTCTCACAAGGGTTGCCCAAGTTCTTGTGGCTGCATTCAGGAAGAGAAAGACTTCATTTTCTAGCAGCGAGATGGTTGGCTTGTACGAGGTTCCCGGCAAGCATTCCGCTATCAAAGGCAGCGGAAAGATCCTTCACACCAATGATTTCAGATGTTTGGACAAGGCAGCACTACAGTTAAAGGAAGATGGAGGCAATCCAAGTCCATGGAGGCTTTGCACAGTGACTCAAGTTGAGGAAGTGAAGATTCTTTTGAAGCTGATTCCAATACCAGCCTGCACAATCATGCTCAATTTAGTGCTAACAGAATATCTCACTCTTTCAGTGCAACAAGCATACACTTTGAACACTCACATGGGCCGTCTTAAGCTCCCTGTCACATGCATGCCAGTCTTCCCTGGCCTCAGCATATTCCTCATACTTTCTCTCTACTACTCAGTATTCGTTCCGCTCTCTCGACGCATCACCGGCCACCCGCACGGTGCTTCTCAGCTACAAAGAGTCGGCATTGGTCTAGCAATCTCGATACTCTCGGTCGCTTGGGCTGGGGCATTCGAGCGATACAGAAGGAACTTTGCTATCAAAAATGGATATGAGGCCAGTTTTTTGACTCCTATGCCCAACTTGACTGCCTACTGGCTGCTCATTCAGTATTGTCTCATTGGCATAGCAGAAGTGTTTTGCATTGTTGGTTTGCTTGAGTTCTTATATGAGGAAGCTCCGGACGCCATGAAGAGCATTGGATCGGCATACGCCGCTCTCGCAGGCGGCTTGGGTTGCTTCGCAGCATCGTTGTTGAACAGTATTATCAAATCCGTCACGGGGAACCCGAACGAAAGAATTCCGTCCTGGCTTTCTCAAAATATTAACACGGGACGATTCGACTACTTCTACTGGCTTCTCACAGTTATGAGTATCATCAATTTCTGCATCTTTCTGTACTCAGCTCATAGGTACAAATACAGGAAAGATCATGAAGTTGGGGAAGGAATCATGGAAAATGGAAGGCATGATAAAATGTGA

Protein sequence

MGSREIKSPQEVIEMPVRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNIRGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSLLLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLTRVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKEDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAGAFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM
Homology
BLAST of Tan0019871 vs. ExPASy Swiss-Prot
Match: Q9LYR6 (Protein NRT1/ PTR FAMILY 6.1 OS=Arabidopsis thaliana OX=3702 GN=NPF6.1 PE=2 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 6.4e-287
Identity = 494/617 (80.06%), Postives = 550/617 (89.14%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPVRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNIRG 60
           M + EIKSP  V E P     H     RK+L +FFIESD+RR A GRGYTGGTTPVNI G
Sbjct: 1   MVASEIKSPVSVPETPGSSSVH----HRKQLSVFFIESDNRRLALGRGYTGGTTPVNIHG 60

Query: 61  KPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG 120
           KPIA+LSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH PF +SS+AVNNFLG
Sbjct: 61  KPIANLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHRPFESSSNAVNNFLG 120

Query: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSLLL 180
           ISQASSVLGGFLADAYLGRYWTIAIFTT+YL GL GITL A++  FVP+Q  C QLSLLL
Sbjct: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTMYLVGLIGITLGASLKMFVPDQSNCGQLSLLL 180

Query: 181 GKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240
           G CE AKSWQM YLYTVLY+TGFGAAGIRPCVSSFGADQFDEKSKDYK+HLDRFFNFFYL
Sbjct: 181 GNCEEAKSWQMLYLYTVLYITGFGAAGIRPCVSSFGADQFDEKSKDYKTHLDRFFNFFYL 240

Query: 241 SVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLTRV 300
           SVT+GAI+AFT VVY+Q++ GWG AFG+LA+AMG SN +FF GTPLYRHRLPGGSPLTRV
Sbjct: 241 SVTLGAIIAFTLVVYVQMELGWGMAFGTLAVAMGISNALFFAGTPLYRHRLPGGSPLTRV 300

Query: 301 AQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKEDGG 360
           AQVLVAAFRKR  +F+SSE +GLYEVPG  SAI GS KI H+NDF  LDKAAL+LKEDG 
Sbjct: 301 AQVLVAAFRKRNAAFTSSEFIGLYEVPGLKSAINGSRKIPHSNDFIWLDKAALELKEDGL 360

Query: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLKLP 420
            PSPW+LCTVTQVEEVKIL++LIPIP CTIML+LVLTEYLTLSVQQAYTLNTH+  LKLP
Sbjct: 361 EPSPWKLCTVTQVEEVKILIRLIPIPTCTIMLSLVLTEYLTLSVQQAYTLNTHIQHLKLP 420

Query: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAGAF 480
           VTCMPVFPGLSIFLILSLYYSVFVP++RRITG+PHGASQLQRVGIGLA+SI+SVAWAG F
Sbjct: 421 VTCMPVFPGLSIFLILSLYYSVFVPITRRITGNPHGASQLQRVGIGLAVSIISVAWAGLF 480

Query: 481 ERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540
           E YRR++AI+NG+E +FLT MP+LTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK
Sbjct: 481 ENYRRHYAIQNGFEFNFLTQMPDLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540

Query: 541 SIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMS 600
           SIGSAYAALAGGLGCFAA++LN+I+K+ T + + +  SWLSQNINTGRFD  YWLLT++S
Sbjct: 541 SIGSAYAALAGGLGCFAATILNNIVKAATRDSDGK--SWLSQNINTGRFDCLYWLLTLLS 600

Query: 601 IINFCIFLYSAHRYKYR 618
            +NFC+FL+SAHRYKYR
Sbjct: 601 FLNFCVFLWSAHRYKYR 611

BLAST of Tan0019871 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 6.5e-114
Identity = 224/568 (39.44%), Postives = 342/568 (60.21%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 115
           V+I   P A+  KTG W A  FI GNE  ER+AY+G+  N+V ++   ++    T+++ V
Sbjct: 13  VDIHKNP-ANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNV 72

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQ 175
            N+ G    + ++G F+ADAYLGRYWTIA F  IY+ G+T +TL A++    P     D 
Sbjct: 73  TNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNAD- 132

Query: 176 LSLLLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                  C P  S Q    +  LY+   G  GI+PCVSSFGADQFDE  ++ K     FF
Sbjct: 133 ------TCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 192

Query: 236 NFFYLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGS 295
           N+FY S+ +GA++A T +V+IQ+  GWG  FG   +AM  +   FF G+  YR + PGGS
Sbjct: 193 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 252

Query: 296 PLTRVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQL 355
           PLTR+ QV+VAAFRK        + + L+E     S IKGS K++HT++ +  DKAA++ 
Sbjct: 253 PLTRIFQVIVAAFRKISVKVPEDKSL-LFETADDESNIKGSRKLVHTDNLKFFDKAAVES 312

Query: 356 KEDG---GNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNT 415
           + D    G  +PWRLC+VTQVEE+K ++ L+P+ A  I+   V ++  T+ V Q  T++ 
Sbjct: 313 QSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQ 372

Query: 416 HMGR-LKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 475
           HMG+  ++P   + +F  +S+     +Y    +PL+R+ T +  G +QLQR+GIGL +SI
Sbjct: 373 HMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSI 432

Query: 476 LSVAWAGAFERYRRNFA-IKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEF 535
            ++  AG  E  R ++    N Y+   +    +++ +W + QY LIG AEVF  +G LEF
Sbjct: 433 FAMITAGVLEVVRLDYVKTHNAYDQKQI----HMSIFWQIPQYLLIGCAEVFTFIGQLEF 492

Query: 536 LYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFD 595
            Y++APDAM+S+ SA +     LG + +++L +++  +T    +  P W+  N+N G  D
Sbjct: 493 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGK--PGWIPDNLNRGHLD 552

Query: 596 YFYWLLTVMSIINFCIFLYSAHRYKYRK 619
           YF++LL  +S +NF ++L+ + RYKY+K
Sbjct: 553 YFFYLLATLSFLNFLVYLWISKRYKYKK 564

BLAST of Tan0019871 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.4e-113
Identity = 232/567 (40.92%), Postives = 337/567 (59.44%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 115
           ++I  KP A+ +KTG W A  FI G E  ER+AY+G+S N++ ++   M+M   ++S +V
Sbjct: 14  LDIHKKP-ANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSV 73

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQ 175
           +N+ G   A+ ++G F+ADAYLGRYWTIA F  IY+ G+T +T+ A++    P    C  
Sbjct: 74  SNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT---CS- 133

Query: 176 LSLLLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                G+   A + Q    +  LYL   G  GI+PCVSSFGADQFD+  +  K     FF
Sbjct: 134 -----GETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 193

Query: 236 NFFYLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGS 295
           N+FY  + +GA++A + +V+IQ+  GWG   G   +AM  + V FF G+  YR + PGGS
Sbjct: 194 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 253

Query: 296 PLTRVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQL 355
           PLTR+ QV+VA+ RK K      E + LYE     S+I GS K+ HT      DKAA++ 
Sbjct: 254 PLTRMLQVIVASCRKSKVKIPEDESL-LYENQDAESSIIGSRKLEHTKILTFFDKAAVET 313

Query: 356 KED---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNT 415
           + D       S W+LCTVTQVEE+K L++L+PI A  I+   V ++  T+ V Q  TL+ 
Sbjct: 314 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 373

Query: 416 HMG-RLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 475
           HMG   K+P   + +F  LS+     +Y  + VP +R+ TGH  G +QLQR+GIGL ISI
Sbjct: 374 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 433

Query: 476 LSVAWAGAFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFL 535
            S+  AG  E  R N+   +        PM   T +W + QY L+G AEVF  +G LEF 
Sbjct: 434 FSMVSAGILEVARLNYVQTHNLYNEETIPM---TIFWQVPQYFLVGCAEVFTFIGQLEFF 493

Query: 536 YEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDY 595
           Y++APDAM+S+ SA +  A   G + ++ L +++  VT +     P W+++N+N G  DY
Sbjct: 494 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGR--PGWIAKNLNNGHLDY 553

Query: 596 FYWLLTVMSIINFCIFLYSAHRYKYRK 619
           F+WLL  +S +NF ++L+ A  Y Y+K
Sbjct: 554 FFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of Tan0019871 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.6e-112
Identity = 229/566 (40.46%), Postives = 336/566 (59.36%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 115
           V+  G P     KTG W A  FI GNE  ER+AY+G++ N++ ++   +H    +++  V
Sbjct: 30  VDFNGNPPLK-EKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 89

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQ 175
             + G    + ++G  LADAY GRYWTIA F+ IY  G++ +TL A++ A  P +     
Sbjct: 90  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE----- 149

Query: 176 LSLLLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
              +   C  A   Q    +  LYL   G  GI+PCVSSFGADQFD+     +     FF
Sbjct: 150 --CIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 209

Query: 236 NFFYLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGS 295
           N+FY S+ IGA+V+ + +V+IQ   GWG  FG   + MG +   FF GTPLYR + PGGS
Sbjct: 210 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 269

Query: 296 PLTRVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQL 355
           P+TR++QV+VA+FRK          + LYE   K+SAI GS KI HT+D + LDKAA+  
Sbjct: 270 PITRISQVVVASFRKSSVKVPEDATL-LYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 329

Query: 356 KED---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNT 415
           +E+   G   + WRLCTVTQVEE+KIL+++ PI A  I+ + V  +  T+ VQQ   +N 
Sbjct: 330 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 389

Query: 416 HMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIL 475
            +G  +LP   +  F   S+ + + LY    VPL+R+ TG   G +++QR+GIGL +S+L
Sbjct: 390 KIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVL 449

Query: 476 SVAWAGAFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLY 535
            +A A   E  R + A   G   S   P+P ++  W + QY ++G AEVF  +G LEF Y
Sbjct: 450 CMAAAAIVEIIRLHMANDLGLVESG-APVP-ISVLWQIPQYFILGAAEVFYFIGQLEFFY 509

Query: 536 EEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYF 595
           +++PDAM+S+ SA A L   LG + +SL+ +++   T    +    W+S N+N+G  DYF
Sbjct: 510 DQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQE--GWISDNLNSGHLDYF 569

Query: 596 YWLLTVMSIINFCIFLYSAHRYKYRK 619
           +WLL  +S++N  ++ +SA RYK +K
Sbjct: 570 FWLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Tan0019871 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.1e-104
Identity = 222/576 (38.54%), Postives = 331/576 (57.47%), Query Frame = 0

Query: 46  GRGYTGGTTPVNIRGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH 105
           G   T     ++I G P     KTG W A  FI GNE  ER+AY+G++ N++ +    +H
Sbjct: 31  GLSSTAEDGSIDIYGNP-PSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELH 90

Query: 106 MPFTTSSDAVNNFLGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISA 165
               +++  V  + G    + ++G  +AD+Y GRYWTIA F+ IY  G+  +TL A++  
Sbjct: 91  ESNVSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPV 150

Query: 166 FVPNQEKCDQLSLLLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSK 225
             P    C  ++  L  C PA + Q    +T LYL   G  GI+PCVSSFGADQFD+   
Sbjct: 151 LKP--AACAGVAAAL--CSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDP 210

Query: 226 DYKSHLDRFFNFFYLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTP 285
             +     FFN+FY S+ IG+ ++ T +V++Q   GWG  F    + MG S   FFIGTP
Sbjct: 211 RERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTP 270

Query: 286 LYRHRLPGGSPLTRVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDF 345
           LYR + PGGSP+TRV QVLVAA+RK K +    ++  LYE   K+S I GS KI HT+ +
Sbjct: 271 LYRFQKPGGSPITRVCQVLVAAYRKLKLNL-PEDISFLYETREKNSMIAGSRKIQHTDGY 330

Query: 346 RCLDKAAL---QLKEDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTL 405
           + LDKAA+      + G   +PW+LCTVTQVEEVK L+++ PI A  I+ +++ ++  TL
Sbjct: 331 KFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTL 390

Query: 406 SVQQAYTLNTHMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQR 465
            VQQ  ++N  +   ++P     VF  L + + + +Y    VP  RR TG P G + LQR
Sbjct: 391 FVQQGRSMNRIIRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQR 450

Query: 466 VGIGLAISILSVAWAGAFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVF 525
           +GIGL +S+LS+A A   E  R   A        F+     ++ +W + QY L+GIAEVF
Sbjct: 451 MGIGLFLSVLSIAAAAIVETVRLQLA------QDFVA----MSIFWQIPQYILMGIAEVF 510

Query: 526 CIVGLLEFLYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQ 585
             +G +EF Y+E+PDAM+S+ SA A L   +G + +SL+ +++   T    +    W+  
Sbjct: 511 FFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGK--DGWVPD 570

Query: 586 NINTGRFDYFYWLLTVMSIINFCIFLYSAHRYKYRK 619
           ++N G  DYF+WLL  + ++N  ++     ++  +K
Sbjct: 571 DLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKK 588

BLAST of Tan0019871 vs. NCBI nr
Match: XP_038903063.1 (protein NRT1/ PTR FAMILY 6.1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 608/637 (95.45%), Postives = 622/637 (97.65%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMP--VRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKSPQEVIE     RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSPQEVIETSGLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPIADLSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF
Sbjct: 61  RGKPIADLSKTGGWVAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATIS FVPNQEKCDQLSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISTFVPNQEKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR  SFS+SE VGLYEVPGK SAIKGSGKILHT+DFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNISFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLK 420
           G NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYT+NTH+G LK
Sbjct: 361 GRNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGHLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAW G
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIMSVAWGG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRN+AI+NGYEASFLTPMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNYAIENGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKS+TGNPNER PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSITGNPNERNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MS INFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM
Sbjct: 601 MSFINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 637

BLAST of Tan0019871 vs. NCBI nr
Match: XP_011653004.1 (protein NRT1/ PTR FAMILY 6.1 [Cucumis sativus] >XP_031740724.1 protein NRT1/ PTR FAMILY 6.1 [Cucumis sativus] >KGN52956.1 hypothetical protein Csa_014689 [Cucumis sativus])

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 602/637 (94.51%), Postives = 620/637 (97.33%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPV--RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS  EV E PV  RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLHEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPIADLSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF
Sbjct: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGL GITLCATIS FVPNQ KCDQLSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAK WQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKPWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR  SFS+SE VGL+EVPGK SAIKGSGKILHT+DFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLFEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYT+NTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFAI++GYEASFL+PMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAIRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+PN R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSPNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAH+YKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHKYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Tan0019871 vs. NCBI nr
Match: KAA0044494.1 (protein NRT1/ PTR FAMILY 6.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1204.5 bits (3115), Expect = 0.0e+00
Identity = 601/637 (94.35%), Postives = 620/637 (97.33%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPV--RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E PV  RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPI DLSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SSDAVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGL GITLCATIS FVPNQ KCDQLSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQI+HGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR  SFS+SE VGLYEVPGK SAIKGSGKILHT+DFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYT+NTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Tan0019871 vs. NCBI nr
Match: XP_008454154.1 (PREDICTED: protein NRT1/ PTR FAMILY 6.1 [Cucumis melo] >XP_008454155.1 PREDICTED: protein NRT1/ PTR FAMILY 6.1 [Cucumis melo] >XP_008454156.1 PREDICTED: protein NRT1/ PTR FAMILY 6.1 [Cucumis melo] >TYK29622.1 protein NRT1/ PTR FAMILY 6.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 601/637 (94.35%), Postives = 620/637 (97.33%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPV--RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E PV  RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPI DLSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SSDAVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGL GITLCATIS FVPNQ KCDQLSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISRFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQI+HGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR  SFS+SE VGLYEVPGK SAIKGSGKILHT+DFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYT+NTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Tan0019871 vs. NCBI nr
Match: XP_022972287.1 (protein NRT1/ PTR FAMILY 6.1 [Cucurbita maxima])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 598/635 (94.17%), Postives = 615/635 (96.85%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPVRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNIRG 60
           MGS EIKSPQE++EMP RLDE     QRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNIRG
Sbjct: 1   MGSTEIKSPQEMVEMPARLDE-----QRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG 60

Query: 61  KPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG 120
           KPIADLSKTGGW AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG
Sbjct: 61  KPIADLSKTGGWTAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG 120

Query: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSLLL 180
           ISQASSVLGGF+ADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQ+ CD+LSLLL
Sbjct: 121 ISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQKICDELSLLL 180

Query: 181 GKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240
           GKCEPAK+WQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL
Sbjct: 181 GKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240

Query: 241 SVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLTRV 300
           SVT GAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLTRV
Sbjct: 241 SVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRV 300

Query: 301 AQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKEDGG 360
           AQVLVAAFRKR  SFSSSEMVGLYEV GKHSAIKGSGKILHTNDFRCLDKAAL+LK+DGG
Sbjct: 301 AQVLVAAFRKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGG 360

Query: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLKLP 420
           NPSPWRLCTVTQVEEVKILLKLIPIPACTI+LNLVLTEYLTLSVQQAYTLNTHMGRLKLP
Sbjct: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHMGRLKLP 420

Query: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAGAF 480
           VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG F
Sbjct: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVF 480

Query: 481 ERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540
           ERYRRN+AIK+GYEASFLTPMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK
Sbjct: 481 ERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540

Query: 541 SIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMS 600
           SIGS+YAALAGGLGCFAASLLNSIIKSVTGNP E  PSWLSQNIN G+FDYFYWLLTVMS
Sbjct: 541 SIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMS 600

Query: 601 IINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           IINFCIFLYSAHRYKYRKDHEVGEGIMENGRH  M
Sbjct: 601 IINFCIFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Tan0019871 vs. ExPASy TrEMBL
Match: A0A0A0KYN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007610 PE=3 SV=1)

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 602/637 (94.51%), Postives = 620/637 (97.33%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPV--RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS  EV E PV  RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLHEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPIADLSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF
Sbjct: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGL GITLCATIS FVPNQ KCDQLSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAK WQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKPWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR  SFS+SE VGL+EVPGK SAIKGSGKILHT+DFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLFEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYT+NTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFAI++GYEASFL+PMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAIRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+PN R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSPNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAH+YKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHKYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Tan0019871 vs. ExPASy TrEMBL
Match: A0A5A7TN06 (Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002320 PE=3 SV=1)

HSP 1 Score: 1204.5 bits (3115), Expect = 0.0e+00
Identity = 601/637 (94.35%), Postives = 620/637 (97.33%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPV--RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E PV  RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPI DLSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SSDAVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGL GITLCATIS FVPNQ KCDQLSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQI+HGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR  SFS+SE VGLYEVPGK SAIKGSGKILHT+DFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYT+NTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Tan0019871 vs. ExPASy TrEMBL
Match: A0A1S3BXF6 (protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo OX=3656 GN=LOC103494642 PE=3 SV=1)

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 601/637 (94.35%), Postives = 620/637 (97.33%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPV--RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E PV  RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPI DLSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SSDAVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGL GITLCATIS FVPNQ KCDQLSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISRFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQI+HGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR  SFS+SE VGLYEVPGK SAIKGSGKILHT+DFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYT+NTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Tan0019871 vs. ExPASy TrEMBL
Match: A0A5D3E115 (Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002340 PE=3 SV=1)

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 601/637 (94.35%), Postives = 620/637 (97.33%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPV--RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E PV  RLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPI DLSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SSDAVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGL GITLCATIS FVPNQ KCDQLSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISRFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQI+HGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR  SFS+SE VGLYEVPGK SAIKGSGKILHT+DFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYT+NTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Tan0019871 vs. ExPASy TrEMBL
Match: A0A6J1I4E7 (protein NRT1/ PTR FAMILY 6.1 OS=Cucurbita maxima OX=3661 GN=LOC111470868 PE=3 SV=1)

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 598/635 (94.17%), Postives = 615/635 (96.85%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPVRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNIRG 60
           MGS EIKSPQE++EMP RLDE     QRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNIRG
Sbjct: 1   MGSTEIKSPQEMVEMPARLDE-----QRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG 60

Query: 61  KPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG 120
           KPIADLSKTGGW AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG
Sbjct: 61  KPIADLSKTGGWTAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG 120

Query: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSLLL 180
           ISQASSVLGGF+ADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQ+ CD+LSLLL
Sbjct: 121 ISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQKICDELSLLL 180

Query: 181 GKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240
           GKCEPAK+WQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL
Sbjct: 181 GKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240

Query: 241 SVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLTRV 300
           SVT GAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFF+GTPLYRHRLPGGSPLTRV
Sbjct: 241 SVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRV 300

Query: 301 AQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKEDGG 360
           AQVLVAAFRKR  SFSSSEMVGLYEV GKHSAIKGSGKILHTNDFRCLDKAAL+LK+DGG
Sbjct: 301 AQVLVAAFRKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGG 360

Query: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLKLP 420
           NPSPWRLCTVTQVEEVKILLKLIPIPACTI+LNLVLTEYLTLSVQQAYTLNTHMGRLKLP
Sbjct: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHMGRLKLP 420

Query: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAGAF 480
           VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG F
Sbjct: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVF 480

Query: 481 ERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540
           ERYRRN+AIK+GYEASFLTPMPNL+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK
Sbjct: 481 ERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540

Query: 541 SIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMS 600
           SIGS+YAALAGGLGCFAASLLNSIIKSVTGNP E  PSWLSQNIN G+FDYFYWLLTVMS
Sbjct: 541 SIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMS 600

Query: 601 IINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           IINFCIFLYSAHRYKYRKDHEVGEGIMENGRH  M
Sbjct: 601 IINFCIFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Tan0019871 vs. TAIR 10
Match: AT5G13400.1 (Major facilitator superfamily protein )

HSP 1 Score: 987.6 bits (2552), Expect = 4.5e-288
Identity = 494/617 (80.06%), Postives = 550/617 (89.14%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPVRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNIRG 60
           M + EIKSP  V E P     H     RK+L +FFIESD+RR A GRGYTGGTTPVNI G
Sbjct: 1   MVASEIKSPVSVPETPGSSSVH----HRKQLSVFFIESDNRRLALGRGYTGGTTPVNIHG 60

Query: 61  KPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG 120
           KPIA+LSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH PF +SS+AVNNFLG
Sbjct: 61  KPIANLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHRPFESSSNAVNNFLG 120

Query: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQLSLLL 180
           ISQASSVLGGFLADAYLGRYWTIAIFTT+YL GL GITL A++  FVP+Q  C QLSLLL
Sbjct: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTMYLVGLIGITLGASLKMFVPDQSNCGQLSLLL 180

Query: 181 GKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240
           G CE AKSWQM YLYTVLY+TGFGAAGIRPCVSSFGADQFDEKSKDYK+HLDRFFNFFYL
Sbjct: 181 GNCEEAKSWQMLYLYTVLYITGFGAAGIRPCVSSFGADQFDEKSKDYKTHLDRFFNFFYL 240

Query: 241 SVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGSPLTRV 300
           SVT+GAI+AFT VVY+Q++ GWG AFG+LA+AMG SN +FF GTPLYRHRLPGGSPLTRV
Sbjct: 241 SVTLGAIIAFTLVVYVQMELGWGMAFGTLAVAMGISNALFFAGTPLYRHRLPGGSPLTRV 300

Query: 301 AQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQLKEDGG 360
           AQVLVAAFRKR  +F+SSE +GLYEVPG  SAI GS KI H+NDF  LDKAAL+LKEDG 
Sbjct: 301 AQVLVAAFRKRNAAFTSSEFIGLYEVPGLKSAINGSRKIPHSNDFIWLDKAALELKEDGL 360

Query: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNTHMGRLKLP 420
            PSPW+LCTVTQVEEVKIL++LIPIP CTIML+LVLTEYLTLSVQQAYTLNTH+  LKLP
Sbjct: 361 EPSPWKLCTVTQVEEVKILIRLIPIPTCTIMLSLVLTEYLTLSVQQAYTLNTHIQHLKLP 420

Query: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAGAF 480
           VTCMPVFPGLSIFLILSLYYSVFVP++RRITG+PHGASQLQRVGIGLA+SI+SVAWAG F
Sbjct: 421 VTCMPVFPGLSIFLILSLYYSVFVPITRRITGNPHGASQLQRVGIGLAVSIISVAWAGLF 480

Query: 481 ERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540
           E YRR++AI+NG+E +FLT MP+LTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK
Sbjct: 481 ENYRRHYAIQNGFEFNFLTQMPDLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540

Query: 541 SIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMS 600
           SIGSAYAALAGGLGCFAA++LN+I+K+ T + + +  SWLSQNINTGRFD  YWLLT++S
Sbjct: 541 SIGSAYAALAGGLGCFAATILNNIVKAATRDSDGK--SWLSQNINTGRFDCLYWLLTLLS 600

Query: 601 IINFCIFLYSAHRYKYR 618
            +NFC+FL+SAHRYKYR
Sbjct: 601 FLNFCVFLWSAHRYKYR 611

BLAST of Tan0019871 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 412.9 bits (1060), Expect = 4.6e-115
Identity = 224/568 (39.44%), Postives = 342/568 (60.21%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 115
           V+I   P A+  KTG W A  FI GNE  ER+AY+G+  N+V ++   ++    T+++ V
Sbjct: 13  VDIHKNP-ANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNV 72

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQ 175
            N+ G    + ++G F+ADAYLGRYWTIA F  IY+ G+T +TL A++    P     D 
Sbjct: 73  TNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNAD- 132

Query: 176 LSLLLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                  C P  S Q    +  LY+   G  GI+PCVSSFGADQFDE  ++ K     FF
Sbjct: 133 ------TCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 192

Query: 236 NFFYLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGS 295
           N+FY S+ +GA++A T +V+IQ+  GWG  FG   +AM  +   FF G+  YR + PGGS
Sbjct: 193 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 252

Query: 296 PLTRVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQL 355
           PLTR+ QV+VAAFRK        + + L+E     S IKGS K++HT++ +  DKAA++ 
Sbjct: 253 PLTRIFQVIVAAFRKISVKVPEDKSL-LFETADDESNIKGSRKLVHTDNLKFFDKAAVES 312

Query: 356 KEDG---GNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNT 415
           + D    G  +PWRLC+VTQVEE+K ++ L+P+ A  I+   V ++  T+ V Q  T++ 
Sbjct: 313 QSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQ 372

Query: 416 HMGR-LKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 475
           HMG+  ++P   + +F  +S+     +Y    +PL+R+ T +  G +QLQR+GIGL +SI
Sbjct: 373 HMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSI 432

Query: 476 LSVAWAGAFERYRRNFA-IKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEF 535
            ++  AG  E  R ++    N Y+   +    +++ +W + QY LIG AEVF  +G LEF
Sbjct: 433 FAMITAGVLEVVRLDYVKTHNAYDQKQI----HMSIFWQIPQYLLIGCAEVFTFIGQLEF 492

Query: 536 LYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFD 595
            Y++APDAM+S+ SA +     LG + +++L +++  +T    +  P W+  N+N G  D
Sbjct: 493 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGK--PGWIPDNLNRGHLD 552

Query: 596 YFYWLLTVMSIINFCIFLYSAHRYKYRK 619
           YF++LL  +S +NF ++L+ + RYKY+K
Sbjct: 553 YFFYLLATLSFLNFLVYLWISKRYKYKK 564

BLAST of Tan0019871 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 411.8 bits (1057), Expect = 1.0e-114
Identity = 232/567 (40.92%), Postives = 337/567 (59.44%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 115
           ++I  KP A+ +KTG W A  FI G E  ER+AY+G+S N++ ++   M+M   ++S +V
Sbjct: 14  LDIHKKP-ANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSV 73

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQ 175
           +N+ G   A+ ++G F+ADAYLGRYWTIA F  IY+ G+T +T+ A++    P    C  
Sbjct: 74  SNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT---CS- 133

Query: 176 LSLLLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                G+   A + Q    +  LYL   G  GI+PCVSSFGADQFD+  +  K     FF
Sbjct: 134 -----GETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 193

Query: 236 NFFYLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGS 295
           N+FY  + +GA++A + +V+IQ+  GWG   G   +AM  + V FF G+  YR + PGGS
Sbjct: 194 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 253

Query: 296 PLTRVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQL 355
           PLTR+ QV+VA+ RK K      E + LYE     S+I GS K+ HT      DKAA++ 
Sbjct: 254 PLTRMLQVIVASCRKSKVKIPEDESL-LYENQDAESSIIGSRKLEHTKILTFFDKAAVET 313

Query: 356 KED---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNT 415
           + D       S W+LCTVTQVEE+K L++L+PI A  I+   V ++  T+ V Q  TL+ 
Sbjct: 314 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 373

Query: 416 HMG-RLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 475
           HMG   K+P   + +F  LS+     +Y  + VP +R+ TGH  G +QLQR+GIGL ISI
Sbjct: 374 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 433

Query: 476 LSVAWAGAFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFL 535
            S+  AG  E  R N+   +        PM   T +W + QY L+G AEVF  +G LEF 
Sbjct: 434 FSMVSAGILEVARLNYVQTHNLYNEETIPM---TIFWQVPQYFLVGCAEVFTFIGQLEFF 493

Query: 536 YEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDY 595
           Y++APDAM+S+ SA +  A   G + ++ L +++  VT +     P W+++N+N G  DY
Sbjct: 494 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGR--PGWIAKNLNNGHLDY 553

Query: 596 FYWLLTVMSIINFCIFLYSAHRYKYRK 619
           F+WLL  +S +NF ++L+ A  Y Y+K
Sbjct: 554 FFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of Tan0019871 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 408.3 bits (1048), Expect = 1.1e-113
Identity = 229/566 (40.46%), Postives = 336/566 (59.36%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 115
           V+  G P     KTG W A  FI GNE  ER+AY+G++ N++ ++   +H    +++  V
Sbjct: 30  VDFNGNPPLK-EKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 89

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQEKCDQ 175
             + G    + ++G  LADAY GRYWTIA F+ IY  G++ +TL A++ A  P +     
Sbjct: 90  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE----- 149

Query: 176 LSLLLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
              +   C  A   Q    +  LYL   G  GI+PCVSSFGADQFD+     +     FF
Sbjct: 150 --CIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 209

Query: 236 NFFYLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTPLYRHRLPGGS 295
           N+FY S+ IGA+V+ + +V+IQ   GWG  FG   + MG +   FF GTPLYR + PGGS
Sbjct: 210 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 269

Query: 296 PLTRVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDFRCLDKAALQL 355
           P+TR++QV+VA+FRK          + LYE   K+SAI GS KI HT+D + LDKAA+  
Sbjct: 270 PITRISQVVVASFRKSSVKVPEDATL-LYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 329

Query: 356 KED---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTLNT 415
           +E+   G   + WRLCTVTQVEE+KIL+++ PI A  I+ + V  +  T+ VQQ   +N 
Sbjct: 330 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 389

Query: 416 HMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIL 475
            +G  +LP   +  F   S+ + + LY    VPL+R+ TG   G +++QR+GIGL +S+L
Sbjct: 390 KIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVL 449

Query: 476 SVAWAGAFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVFCIVGLLEFLY 535
            +A A   E  R + A   G   S   P+P ++  W + QY ++G AEVF  +G LEF Y
Sbjct: 450 CMAAAAIVEIIRLHMANDLGLVESG-APVP-ISVLWQIPQYFILGAAEVFYFIGQLEFFY 509

Query: 536 EEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYF 595
           +++PDAM+S+ SA A L   LG + +SL+ +++   T    +    W+S N+N+G  DYF
Sbjct: 510 DQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQE--GWISDNLNSGHLDYF 569

Query: 596 YWLLTVMSIINFCIFLYSAHRYKYRK 619
           +WLL  +S++N  ++ +SA RYK +K
Sbjct: 570 FWLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Tan0019871 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 381.3 bits (978), Expect = 1.5e-105
Identity = 222/576 (38.54%), Postives = 331/576 (57.47%), Query Frame = 0

Query: 46  GRGYTGGTTPVNIRGKPIADLSKTGGWLAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH 105
           G   T     ++I G P     KTG W A  FI GNE  ER+AY+G++ N++ +    +H
Sbjct: 31  GLSSTAEDGSIDIYGNP-PSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELH 90

Query: 106 MPFTTSSDAVNNFLGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISA 165
               +++  V  + G    + ++G  +AD+Y GRYWTIA F+ IY  G+  +TL A++  
Sbjct: 91  ESNVSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPV 150

Query: 166 FVPNQEKCDQLSLLLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSK 225
             P    C  ++  L  C PA + Q    +T LYL   G  GI+PCVSSFGADQFD+   
Sbjct: 151 LKP--AACAGVAAAL--CSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDP 210

Query: 226 DYKSHLDRFFNFFYLSVTIGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFIGTP 285
             +     FFN+FY S+ IG+ ++ T +V++Q   GWG  F    + MG S   FFIGTP
Sbjct: 211 RERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTP 270

Query: 286 LYRHRLPGGSPLTRVAQVLVAAFRKRKTSFSSSEMVGLYEVPGKHSAIKGSGKILHTNDF 345
           LYR + PGGSP+TRV QVLVAA+RK K +    ++  LYE   K+S I GS KI HT+ +
Sbjct: 271 LYRFQKPGGSPITRVCQVLVAAYRKLKLNL-PEDISFLYETREKNSMIAGSRKIQHTDGY 330

Query: 346 RCLDKAAL---QLKEDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTL 405
           + LDKAA+      + G   +PW+LCTVTQVEEVK L+++ PI A  I+ +++ ++  TL
Sbjct: 331 KFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTL 390

Query: 406 SVQQAYTLNTHMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQR 465
            VQQ  ++N  +   ++P     VF  L + + + +Y    VP  RR TG P G + LQR
Sbjct: 391 FVQQGRSMNRIIRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQR 450

Query: 466 VGIGLAISILSVAWAGAFERYRRNFAIKNGYEASFLTPMPNLTAYWLLIQYCLIGIAEVF 525
           +GIGL +S+LS+A A   E  R   A        F+     ++ +W + QY L+GIAEVF
Sbjct: 451 MGIGLFLSVLSIAAAAIVETVRLQLA------QDFVA----MSIFWQIPQYILMGIAEVF 510

Query: 526 CIVGLLEFLYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQ 585
             +G +EF Y+E+PDAM+S+ SA A L   +G + +SL+ +++   T    +    W+  
Sbjct: 511 FFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGK--DGWVPD 570

Query: 586 NINTGRFDYFYWLLTVMSIINFCIFLYSAHRYKYRK 619
           ++N G  DYF+WLL  + ++N  ++     ++  +K
Sbjct: 571 DLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKK 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYR66.4e-28780.06Protein NRT1/ PTR FAMILY 6.1 OS=Arabidopsis thaliana OX=3702 GN=NPF6.1 PE=2 SV=1[more]
Q9M3906.5e-11439.44Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB81.4e-11340.92Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
P460321.6e-11240.46Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z202.1e-10438.54Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038903063.10.0e+0095.45protein NRT1/ PTR FAMILY 6.1 isoform X1 [Benincasa hispida][more]
XP_011653004.10.0e+0094.51protein NRT1/ PTR FAMILY 6.1 [Cucumis sativus] >XP_031740724.1 protein NRT1/ PTR... [more]
KAA0044494.10.0e+0094.35protein NRT1/ PTR FAMILY 6.1 [Cucumis melo var. makuwa][more]
XP_008454154.10.0e+0094.35PREDICTED: protein NRT1/ PTR FAMILY 6.1 [Cucumis melo] >XP_008454155.1 PREDICTED... [more]
XP_022972287.10.0e+0094.17protein NRT1/ PTR FAMILY 6.1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0KYN30.0e+0094.51Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007610 PE=3 SV=1[more]
A0A5A7TN060.0e+0094.35Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BXF60.0e+0094.35protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo OX=3656 GN=LOC103494642 PE=3 SV=1[more]
A0A5D3E1150.0e+0094.35Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1I4E70.0e+0094.17protein NRT1/ PTR FAMILY 6.1 OS=Cucurbita maxima OX=3661 GN=LOC111470868 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT5G13400.14.5e-28880.06Major facilitator superfamily protein [more]
AT3G54140.14.6e-11539.44peptide transporter 1 [more]
AT5G01180.11.0e-11440.92peptide transporter 5 [more]
AT2G02040.11.1e-11340.46peptide transporter 2 [more]
AT1G62200.11.5e-10538.54Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..472
e-value: 1.1E-127
score: 428.4
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 39..468
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 15..421
e-value: 2.5E-77
score: 260.4
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 3..467
NoneNo IPR availablePANTHERPTHR11654:SF112PROTEIN NRT1/ PTR FAMILY 6.1coord: 3..467

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0019871.1Tan0019871.1mRNA
Tan0019871.2Tan0019871.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity