Tan0019750 (gene) Snake gourd v1

Overview
NameTan0019750
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionglutelin type-A 2-like
LocationLG01: 7211718 .. 7213892 (+)
RNA-Seq ExpressionTan0019750
SyntenyTan0019750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGGTATCAAATTCAGCTCTAGAAAATAATAATAATAATAATAATAATCTCATTATTGAACTTGGTCCATTAATTCTTCATTAATTATTTGAATGGAGCCAATGAGTCCCAAGCCCTTCTCTGAGGGAGATGGTGGATCCTATCTCAAATGGTTGCCTTCTGATTATCCCTTGCTTGCTCAAACCAACGTCGCCGCCGGCCGCCTTCTCCTCCGCCGTTGCGGCTTCGCCCTTCCTCACTATGCCGATTGTTCCAAAGTGGGCTACGTTCTTCAAGGTAAAACCTACTCGCTATAGATAACGATTTCAGGACTGGTGTTGAAATAATCTTAGTAAACAGCACAACTATACTTTTAAGTTTTAAACCTTTCATGTGACGTCCGAGGAAGAGGGGTACATGAATAAACCGAGTCTACATCTGAATGAGAGGGATCGAGGATATGAAAGTATGGTGCTCGAGGAAGTGAAAGTATAGTTGAGAAAATCTTAAAAAAAATTGATAGGTATTACCTTTACCAACAAGGTGCACTTTCCTTGTCGGTGGCTCAATCATAGGAACTCTACAATTACAATTAGGCGTGCTTGGCTTGGAGCAATGCTATGTTGGGTGACCTTCTAAGAATTTTCTTATGGAACATGTGAGTGAGGACAAAACATGTTGAAATGACTCCGTGTTGGTTTGTGAGGATAGTATCTTCACACTTACAAACAATCATGGATGATATGGTCTCAAACAACACAAACAGAATGGGGCGCACTCCAAGAGCAGAAACCAAGCGTTCTTGTGCTCTCCCCATCAACTATATTTTTTAAAAAATTTAGAAAACTCGATAACCCAACCAATCCAAAGGTTGGGTTCAAATTAAAATGTATGAGTTGGATTGGGTTGATTTGTGGGTTCACCTAAAATATATATATTTTGTTGTGCAAGTTTAAATTTGTTTGTTCTTAAAAGTGTTTGATATAGAATGATGCAATTTAGGTCTAGCAATAAATTAAATGGTGCGGTGTGTATCAGGTAACGATGGAGTTGCAGGATTCGTATTTCCAAACAAGTCCGACGAGGTGGTGGTGAAGTTAAATAAAGGAGATTTGATTCCAGTGCCGAATGGAACCACTTCGTGGTGGTTCAACCATGGCGATTCCGATCTGGAAATAGTCTTTTTGGGTGAAACCAAAGGCGGTCATGTCGCCGGAGACTTCACTTACTATTTGCTCCCCGGCCCCGCCGGTCTCCTACAAGGCTTCTCGCCGGAGTACATCGGAAAAGCCTACCATCTAAACGAACAAGAAACAACCACACTTCTCAAAACCCAACCCAACCCCTTAATCTTCACCATTCCACAATCTGATCAATCACAGCTTCCCAAACCCCATCAACAAAGTAAACTAGTTTATAACATTGACGCCGCCGCGCCGGACACTATACCCAATGATGGCACCCTCGCCGTCACCACGGTCACGGAATCCAAATTTCCCTTCATTGGACAATCTGGGTTGACGGCAATTCTCGAAAAACTCGACGCTAACGCCGTTCAATCGCCGAGCTATGTCGCTCAGCCGTCAGATCAACTGATCTACGTGGCTAACGGATCCGGGAAGATTCAGATCGTCGGATTTTCCGGTAAATTTTTTGATGCAGAGGTGAAAATGGGTCAGCTGATTTTGGTCCCCAAATATTTCGCCGCCGGAAAAATCGCCGGAGATGAAGGCTTGGAGTGCATTTCCATTATCGTAGCTACAAAGTAAAAATCTTCAAAATTTTTCTTATTGGGTTTTTTCTTTAAATCTTCAAAATTTAACGATTTTCTTTTTTTTTTCATAATTTGAACAGTCCACGAGTGGAAGAATTGGCCGGAAAGAGGTCGGTTTTGGAGGCTTTATCGCCGGTGGTTTTTCAAATTTCGTTCAACGTGACGGCGGAGTTCGAGAAGCTTTTCAGGTCAAAGGCATAACAAAAGTTTCCCCTTTGGTTTGGTCAATATTGCGACATGATCCTATAGTTCTATGCTAAAAAAAATAAAAAGTAATATTATATATTATAATATTTGTTTTATACTTTGAAGCTTTGTGCGAGGCCAAAAAGAGCAAATTTCAACAAGCCTAAAATATAGACTTTGGATCATAATGTCACATATTCGATCCTCTTATCTTAAATATTATTAAGCTCAAAT

mRNA sequence

TTTTGGTATCAAATTCAGCTCTAGAAAATAATAATAATAATAATAATAATCTCATTATTGAACTTGGTCCATTAATTCTTCATTAATTATTTGAATGGAGCCAATGAGTCCCAAGCCCTTCTCTGAGGGAGATGGTGGATCCTATCTCAAATGGTTGCCTTCTGATTATCCCTTGCTTGCTCAAACCAACGTCGCCGCCGGCCGCCTTCTCCTCCGCCGTTGCGGCTTCGCCCTTCCTCACTATGCCGATTGTTCCAAAGTGGGCTACGTTCTTCAAGGTAACGATGGAGTTGCAGGATTCGTATTTCCAAACAAGTCCGACGAGGTGGTGGTGAAGTTAAATAAAGGAGATTTGATTCCAGTGCCGAATGGAACCACTTCGTGGTGGTTCAACCATGGCGATTCCGATCTGGAAATAGTCTTTTTGGGTGAAACCAAAGGCGGTCATGTCGCCGGAGACTTCACTTACTATTTGCTCCCCGGCCCCGCCGGTCTCCTACAAGGCTTCTCGCCGGAGTACATCGGAAAAGCCTACCATCTAAACGAACAAGAAACAACCACACTTCTCAAAACCCAACCCAACCCCTTAATCTTCACCATTCCACAATCTGATCAATCACAGCTTCCCAAACCCCATCAACAAAGTAAACTAGTTTATAACATTGACGCCGCCGCGCCGGACACTATACCCAATGATGGCACCCTCGCCGTCACCACGGTCACGGAATCCAAATTTCCCTTCATTGGACAATCTGGGTTGACGGCAATTCTCGAAAAACTCGACGCTAACGCCGTTCAATCGCCGAGCTATGTCGCTCAGCCGTCAGATCAACTGATCTACGTGGCTAACGGATCCGGGAAGATTCAGATCGTCGGATTTTCCGGTAAATTTTTTGATGCAGAGGTGAAAATGGGTCAGCTGATTTTGGTCCCCAAATATTTCGCCGCCGGAAAAATCGCCGGAGATGAAGGCTTGGAGTGCATTTCCATTATCGTAGCTACAAATCCACGAGTGGAAGAATTGGCCGGAAAGAGGTCGGTTTTGGAGGCTTTATCGCCGGTGGTTTTTCAAATTTCGTTCAACGTGACGGCGGAGTTCGAGAAGCTTTTCAGGTCAAAGGCATAACAAAAGTTTCCCCTTTGGTTTGGTCAATATTGCGACATGATCCTATAGTTCTATGCTAAAAAAAATAAAAAGTAATATTATATATTATAATATTTGTTTTATACTTTGAAGCTTTGTGCGAGGCCAAAAAGAGCAAATTTCAACAAGCCTAAAATATAGACTTTGGATCATAATGTCACATATTCGATCCTCTTATCTTAAATATTATTAAGCTCAAAT

Coding sequence (CDS)

ATGGAGCCAATGAGTCCCAAGCCCTTCTCTGAGGGAGATGGTGGATCCTATCTCAAATGGTTGCCTTCTGATTATCCCTTGCTTGCTCAAACCAACGTCGCCGCCGGCCGCCTTCTCCTCCGCCGTTGCGGCTTCGCCCTTCCTCACTATGCCGATTGTTCCAAAGTGGGCTACGTTCTTCAAGGTAACGATGGAGTTGCAGGATTCGTATTTCCAAACAAGTCCGACGAGGTGGTGGTGAAGTTAAATAAAGGAGATTTGATTCCAGTGCCGAATGGAACCACTTCGTGGTGGTTCAACCATGGCGATTCCGATCTGGAAATAGTCTTTTTGGGTGAAACCAAAGGCGGTCATGTCGCCGGAGACTTCACTTACTATTTGCTCCCCGGCCCCGCCGGTCTCCTACAAGGCTTCTCGCCGGAGTACATCGGAAAAGCCTACCATCTAAACGAACAAGAAACAACCACACTTCTCAAAACCCAACCCAACCCCTTAATCTTCACCATTCCACAATCTGATCAATCACAGCTTCCCAAACCCCATCAACAAAGTAAACTAGTTTATAACATTGACGCCGCCGCGCCGGACACTATACCCAATGATGGCACCCTCGCCGTCACCACGGTCACGGAATCCAAATTTCCCTTCATTGGACAATCTGGGTTGACGGCAATTCTCGAAAAACTCGACGCTAACGCCGTTCAATCGCCGAGCTATGTCGCTCAGCCGTCAGATCAACTGATCTACGTGGCTAACGGATCCGGGAAGATTCAGATCGTCGGATTTTCCGGTAAATTTTTTGATGCAGAGGTGAAAATGGGTCAGCTGATTTTGGTCCCCAAATATTTCGCCGCCGGAAAAATCGCCGGAGATGAAGGCTTGGAGTGCATTTCCATTATCGTAGCTACAAATCCACGAGTGGAAGAATTGGCCGGAAAGAGGTCGGTTTTGGAGGCTTTATCGCCGGTGGTTTTTCAAATTTCGTTCAACGTGACGGCGGAGTTCGAGAAGCTTTTCAGGTCAAAGGCATAA

Protein sequence

MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVLQGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVAGDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKPHQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYVAQPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISIIVATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSKA
Homology
BLAST of Tan0019750 vs. ExPASy Swiss-Prot
Match: Q9ZWA9 (12S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.6e-18
Identity = 86/324 (26.54%), Postives = 140/324 (43.21%), Query Frame = 0

Query: 53  CSKVGYVLQGNDGVAGFVFPNKSDE----VVVKLNKGDLIPVPNGTTSWWFNHGDSDLEI 112
           C +    ++G+ G  G   P +  E     +    +GD+     G + WW+N GDSD  I
Sbjct: 112 CPETFAEVEGSSGRGGGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYNRGDSDAVI 171

Query: 113 VFLGETKGG-----------HVAGDFTY---YLLPGPAG--LLQGFSPEYIGKAYHLNEQ 172
           V + +                +AG  T      L  P+G     GF P  I +A+ +N +
Sbjct: 172 VIVLDVTNRENQLDQVPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIAEAFKINIE 231

Query: 173 ETTTLLKTQPN---------PLIFTIPQSDQSQ-------LPKPHQQSKLVYNIDAAAPD 232
               L   + N         PL F IP   + Q       + + +  +K+  NID   P+
Sbjct: 232 TAKQLQNQKDNRGNIIRANGPLHFVIPPPREWQQDGIANGIEETYCTAKIHENID--DPE 291

Query: 233 TIPNDGTLA--VTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYVAQPSDQLIYVANGS 292
              +  T A  ++T+     P +    L A+   L +  +  P + A  +  ++YV  G 
Sbjct: 292 RSDHFSTRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTAN-AHTVLYVTGGQ 351

Query: 293 GKIQIVGFSGK-FFDAEVKMGQLILVPKYFAAGKIAGDEGLECISIIVATNPRVEELAGK 338
            KIQ+V  +G+  F+ +V  GQ+I++P+ FA  K AG+ G E IS     N  +  L+G+
Sbjct: 352 AKIQVVDDNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYINTLSGQ 411

BLAST of Tan0019750 vs. ExPASy Swiss-Prot
Match: P15456 (12S seed storage protein CRB OS=Arabidopsis thaliana OX=3702 GN=CRB PE=1 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 4.0e-17
Identity = 89/400 (22.25%), Postives = 157/400 (39.25%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           +  + P    + +GG    W     P L  +  A  R ++   G  LP + +  K+ +V+
Sbjct: 35  LNALEPSQIIKSEGGRIEVW-DHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVV 94

Query: 61  QGNDGVAGFVFPNKSD------------------------EVVVKLNKGDLIPVPNGTTS 120
            G  G+ G V P  ++                        + V  L  GD I  P+G   
Sbjct: 95  HGR-GLMGRVIPGCAETFMESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTIATPSGVAQ 154

Query: 121 WWFNHGDSDLEIVFLGE--TKGGHVAGDFTYYLLPG--PAG--------------LLQGF 180
           W++N+G+  L +V   +  +    +  +   +L+ G  P G              +  GF
Sbjct: 155 WFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGF 214

Query: 181 SPEYIGKAYHLNEQETTTLLKTQPN---------PL-IFTIPQSDQSQLPKPHQ------ 240
           +PE + +A+ +N +    L   Q N         P  +   P        +PH+      
Sbjct: 215 APEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRPPLRRGEGGQQPHEIANGLE 274

Query: 241 ----QSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPS 300
                 +   N+D  +   +       ++T+     P +    L+A+   +  NA+  P 
Sbjct: 275 ETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRLLRLSALRGSIRKNAMVLPQ 334

Query: 301 YVAQPSDQLIYVANGSGKIQIVGFSG-KFFDAEVKMGQLILVPKYFAAGKIAGDEGLECI 338
           +    ++  +YV NG   IQ+V  +G + FD E+  GQL++VP+ F+  K A  E  E I
Sbjct: 335 WNVN-ANAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQGFSVMKHAIGEQFEWI 394

BLAST of Tan0019750 vs. ExPASy Swiss-Prot
Match: A0A222NNM9 (Cocosin 1 OS=Cocos nucifera OX=13894 GN=COS-1 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.4e-16
Identity = 79/362 (21.82%), Postives = 148/362 (40.88%), Query Frame = 0

Query: 33  VAAGRLLLRRCGFALPHYADCSKVGYVLQGNDGVAGFVFP-------------------- 92
           V+  R ++   G  LP  ++  ++ Y++QG  G+ G V P                    
Sbjct: 81  VSTIRRVIEPRGLLLPSMSNAPRLVYIVQGR-GIVGLVMPGCPETFQSFQRSEREEGERH 140

Query: 93  ---NKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVAGDFTY--YL 152
                  + V +  +GD++ VPNG   W +N+G++ +  + + +T       D ++  +L
Sbjct: 141 RWSRDEHQKVYQFQEGDVLAVPNGFAYWCYNNGENPVVAITVLDTSNDANQLDRSHRQFL 200

Query: 153 LPG---------------PAGLLQGFSPEYIGKAYHLN---------EQETTTLLKTQPN 212
           L G                  +L+GFS E +  A+ +N           +T   +    N
Sbjct: 201 LAGRQEQGRQRYGREGSIKENILRGFSTELLAAAFGVNMELARKLQCRDDTRGEIVRAEN 260

Query: 213 PLIFTIPQSDQSQ----------LPKPHQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESK 272
            L    P   + +            + +   K+  NI       + N     +TT+   K
Sbjct: 261 GLQVLRPSGMEEEEREEGRSINGFEETYCSMKIKQNIGDPRRADVFNPRGGRITTLNSEK 320

Query: 273 FPFIGQSGLTAILEKLDANAVQSPSYVAQPSDQLIYVANGSGKIQIVGFSGK-FFDAEVK 332
            P +    ++A    L  NA+ SP +    +  ++Y   G G++++    G+  FD E++
Sbjct: 321 LPILRFIQMSAERVVLYRNAMVSPHWNIN-AHSIMYCTGGRGRVEVADDRGETVFDGELR 380

Query: 333 MGQLILVPKYFAAGKIAGDEGLECISIIVATNPRVEELAGKRSVLEALSPVVFQISFNVT 335
            GQL++VP+ FA  + AG EG + +SI  +    V  + GK S L  +   V   S+ ++
Sbjct: 381 QGQLLIVPQNFAMLERAGSEGFQLVSIKTSDRAMVSTIVGKTSALRGMPVEVLMNSYRLS 440

BLAST of Tan0019750 vs. ExPASy Swiss-Prot
Match: P07728 (Glutelin type-A 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA1 PE=1 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 9.8e-16
Identity = 83/388 (21.39%), Postives = 152/388 (39.18%), Query Frame = 0

Query: 31  TNVAAGRLLLRRCGFALPHYADCSKVGYVLQGNDGVAGFVFP------------------ 90
           T V+  R ++   G  LPHY + + + Y++QG  G+ G  FP                  
Sbjct: 80  TGVSVVRRVIEPRGLLLPHYTNGASLVYIIQGR-GITGPTFPGCPESYQQQFQQSGQAQL 139

Query: 91  ----------NKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVAGD 150
                         + + +  +GD+I +P G   W +N G+  +  +++ +   G    D
Sbjct: 140 TESQSQSQKFKDEHQKIHRFRQGDVIALPAGVAHWCYNDGEVPVVAIYVTDLNNGANQLD 199

Query: 151 FTY--YLLPG---------------PAGLLQGFSPEYIGKAYHLNEQETTTL--LKTQPN 210
                +LL G                  +  GFS E + +A  ++ Q    L     Q  
Sbjct: 200 PRQRDFLLAGNKRNPQAYRREVEERSQNIFSGFSTELLSEALGVSSQVARQLQCQNDQRG 259

Query: 211 PLI------------FTIPQSDQSQLPK-------PHQQS-----------------KLV 270
            ++             ++ + +Q Q+          +QQS                 ++ 
Sbjct: 260 EIVRVEHGLSLLQPYASLQEQEQGQVQSRERYQEGQYQQSQYGSGCSNGLDETFCTLRVR 319

Query: 271 YNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYVAQPSDQL 330
            NID        N     VT +    FP +    ++A+   L  NA+ SP +    +  +
Sbjct: 320 QNIDNPNRADTYNPRAGRVTNLNTQNFPILSLVQMSAVKVNLYQNALLSPFWNIN-AHSV 379

Query: 331 IYVANGSGKIQIVGFSGK-FFDAEVKMGQLILVPKYFAAGKIAGDEGLECISIIVATNPR 335
           +Y+  G  ++Q+V  +GK  F+ E++ GQL+++P+++A  K A  EG   I+     N  
Sbjct: 380 VYITQGRARVQVVNNNGKTVFNGELRRGQLLIIPQHYAVVKKAQREGCAYIAFKTNPNSM 439

BLAST of Tan0019750 vs. ExPASy Swiss-Prot
Match: P13744 (11S globulin subunit beta OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.7e-15
Identity = 82/372 (22.04%), Postives = 144/372 (38.71%), Query Frame = 0

Query: 37  RLLLRRCGFALPHYADCSKVGYVLQGNDGVAGFVFPNKSDEVVVKLNK------------ 96
           R  +R  G  LP +++  K+ +V QG  G+ G   P  ++     L +            
Sbjct: 88  RHTIRPKGLLLPGFSNAPKLIFVAQG-FGIRGIAIPGCAETYQTDLRRSQSAGSAFKDQH 147

Query: 97  --------GDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVAGDFTYYL--------- 156
                   GDL+ VP G + W +N G SDL ++   +T+  +VA     YL         
Sbjct: 148 QKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIVFADTR--NVANQIDPYLRKFYLAGRP 207

Query: 157 --------------LPGPAG-----LLQGFSPEYIGKAYHLN---------EQETTTLLK 216
                           G +G     +  GF+ E++ +A+ ++         E +    + 
Sbjct: 208 EQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLEEAFQIDGGLVRKLKGEDDERDRIV 267

Query: 217 TQPNPLIFTIPQSDQSQ----------------LPKPHQQSKLVYNIDAAAPDTIPNDGT 276
                    +P+ D+ +                L +     +L  NI  +    + N   
Sbjct: 268 QVDEDFEVLLPEKDEEERSRGRYIESESESENGLEETICTLRLKQNIGRSVRADVFNPRG 327

Query: 277 LAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYVAQPSDQLIYVANGSGKIQIV-GF 335
             ++T      P + Q  L+A    L +NA+ +P Y    S  ++Y   G+ ++Q+V  F
Sbjct: 328 GRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHYTVN-SHSVMYATRGNARVQVVDNF 387

BLAST of Tan0019750 vs. NCBI nr
Match: XP_008456076.1 (PREDICTED: glutelin type-A 2-like [Cucumis melo] >KAA0039043.1 glutelin type-A 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 524.6 bits (1350), Expect = 6.3e-145
Identity = 257/342 (75.15%), Postives = 290/342 (84.80%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           ME M+PKPF EG+GGSYLKWLPSDYPLLAQTNVA GRLLLR  GFA+PHYADCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVA 120
           QG DGV GFVFPNK +EVV+KL KGDLIPVP+G TSWWFN GDSDLEI+FLGETK  HV 
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 121 GDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKP 180
           GD TY++L GP GLLQGF+PEY+ K+Y L+++ET   LK+Q N LIFT+  S    LPKP
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPS--QSLPKP 180

Query: 181 HQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYV 240
           H+ SKLVYNIDAA PD     G  AVT VTES FPFIGQ+GLTA+LEKLDANA++SP Y+
Sbjct: 181 HKHSKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYI 240

Query: 241 AQPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISII 300
           A+PSDQLIYV  GSGKIQ+VGFS K FDA+VK+GQLILVP+YFA GK+AG+EGLECIS+I
Sbjct: 241 AEPSDQLIYVTKGSGKIQVVGFSSK-FDADVKIGQLILVPRYFAVGKMAGEEGLECISMI 300

Query: 301 VATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSK 343
           VAT+P VEELAGK SVLEALS  VFQ+SFNVTAEFEKLFRSK
Sbjct: 301 VATHPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSK 339

BLAST of Tan0019750 vs. NCBI nr
Match: TYJ99759.1 (glutelin type-A 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 521.2 bits (1341), Expect = 6.9e-144
Identity = 255/340 (75.00%), Postives = 288/340 (84.71%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           ME M+PKPF EG+GGSYLKWLPSDYPLLAQTNVA GRLLLR  GFA+PHYADCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVA 120
           QG DGV GFVFPNK +EVV+KL KGDLIPVP+G TSWWFN GDSDLEI+FLGETK  HV 
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 121 GDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKP 180
           GD TY++L GP GLLQGF+PEY+ K+Y L+++ET   LK+Q N LIFT+  S    LPKP
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPS--QSLPKP 180

Query: 181 HQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYV 240
           H+ SKLVYNIDAA PD     G  AVT VTES FPFIGQ+GLTA+LEKLDANA++SP Y+
Sbjct: 181 HKHSKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYI 240

Query: 241 AQPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISII 300
           A+PSDQLIYV  GSGKIQ+VGFS K FDA+VK+GQLILVP+YFA GK+AG+EGLECIS+I
Sbjct: 241 AEPSDQLIYVTKGSGKIQVVGFSSK-FDADVKIGQLILVPRYFAVGKMAGEEGLECISMI 300

Query: 301 VATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFR 341
           VAT+P VEELAGK SVLEALS  VFQ+SFNVTAEFEKLFR
Sbjct: 301 VATHPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFR 337

BLAST of Tan0019750 vs. NCBI nr
Match: XP_004151504.1 (legumin J [Cucumis sativus] >KGN57580.1 hypothetical protein Csa_009841 [Cucumis sativus])

HSP 1 Score: 520.8 bits (1340), Expect = 9.1e-144
Identity = 257/342 (75.15%), Postives = 287/342 (83.92%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           ME M+PKPF EG+GGSY KWLPSDYPLLAQTNVA GRLLLR  GFA+PHY+DCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYHKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYSDCSKFGYVL 60

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVA 120
           QG DGV GFVFP K +EVV+KL KGDLIPVP G TSWWFN GDSDLEI+FLGETK  HV 
Sbjct: 61  QGEDGVTGFVFPKKCNEVVIKLKKGDLIPVPAGVTSWWFNDGDSDLEIIFLGETKRAHVP 120

Query: 121 GDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKP 180
           GD TY++L GP GLLQGF+PEY+ K+  LN++ET T LK+QPN LIFT+  S    LPKP
Sbjct: 121 GDITYFILSGPRGLLQGFTPEYVQKSCSLNQEETNTFLKSQPNVLIFTVQPS--QSLPKP 180

Query: 181 HQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYV 240
           H+ SKLVYNIDAAAPD     G  AVT VTES FPFIGQ+GLT +LEKLDANA++SP Y+
Sbjct: 181 HKYSKLVYNIDAAAPDNRAKVGDAAVTMVTESTFPFIGQTGLTPVLEKLDANAIRSPVYI 240

Query: 241 AQPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISII 300
           A+PSDQLIYV  GSGKIQ+VGFS K FDA+VK GQLILVP+YFA GKIAG+EGLECIS+I
Sbjct: 241 AEPSDQLIYVTKGSGKIQVVGFSSK-FDADVKTGQLILVPRYFAVGKIAGEEGLECISMI 300

Query: 301 VATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSK 343
           VAT+P VEELAGK SVLEALS  VFQ+SFNVTAEFEKLFRSK
Sbjct: 301 VATHPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSK 339

BLAST of Tan0019750 vs. NCBI nr
Match: XP_022922755.1 (legumin J-like [Cucurbita moschata])

HSP 1 Score: 505.0 bits (1299), Expect = 5.2e-139
Identity = 254/341 (74.49%), Postives = 283/341 (82.99%), Query Frame = 0

Query: 2   EPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVLQ 61
           +PM+PKPF+E + GSY KWLPS+YPLLAQ  VAAGRLLLR  GF +PHYADCSKVGYVLQ
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAQNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 62  GNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVAG 121
           G +GVAG VFP+KSDEVVV L KGDLIPVPNG +SWWFN GDSDLEI+FLGE+K  HV G
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 122 DFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKPH 181
           D +Y++L GP  LL GFSPEY+GK Y LN +ETT  LK+Q N LIF+I Q+    LPKP 
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQT--QSLPKPS 182

Query: 182 QQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYVA 241
           + SK VYNIDAAAPD     G  AVTTVTESKFPFIGQSGLTAILEKL+ANAV+SP YVA
Sbjct: 183 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLNANAVRSPVYVA 242

Query: 242 QPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISIIV 301
           +P DQLIYVA G GKIQIVG S K  DAEVKMGQLILVPK+FA GKIAG++GLECISII 
Sbjct: 243 EPYDQLIYVAKGRGKIQIVGSSSK-IDAEVKMGQLILVPKFFAVGKIAGEDGLECISIIT 302

Query: 302 ATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSK 343
           AT+P VEELAGK SVLEALSP +FQ+SFNVTAEFEKL RSK
Sbjct: 303 ATHPVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSK 340

BLAST of Tan0019750 vs. NCBI nr
Match: KAG6576976.1 (12S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 505.0 bits (1299), Expect = 5.2e-139
Identity = 254/341 (74.49%), Postives = 283/341 (82.99%), Query Frame = 0

Query: 2   EPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVLQ 61
           +PM+PKPF+E + GSY KWLPS+YPLLA+  VAAGRLLLR  GF +PHYADCSKVGYVLQ
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLARNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 62  GNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVAG 121
           G +GVAG VFP+KSDEVVV L KGDLIPVPNG +SWWFN GDSDLEI+FLGE+K  HV G
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNEGDSDLEIIFLGESKNAHVPG 122

Query: 122 DFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKPH 181
           D +Y++L GP  LL GFSPEY+GK Y LN +ETT  LK+Q N LIF+I Q+    LPKP 
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQT--QSLPKPS 182

Query: 182 QQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYVA 241
           + SK VYNIDAAAPD     G  AVTTVTESKFPFIGQSGLTAILEKLDANAV+SP YVA
Sbjct: 183 KFSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 242

Query: 242 QPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISIIV 301
           +P DQLIYVA G GKIQIVG S K  DAEVKMGQLILVPK+FA GKIAG++GLECISII 
Sbjct: 243 EPYDQLIYVAKGRGKIQIVGSSSK-IDAEVKMGQLILVPKFFAVGKIAGEDGLECISIIT 302

Query: 302 ATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSK 343
           AT+P VEELAGK SVLEALSP +FQ+SFNVTAEFEKL RSK
Sbjct: 303 ATHPVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSK 340

BLAST of Tan0019750 vs. ExPASy TrEMBL
Match: A0A5A7T7U8 (Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001330 PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 3.0e-145
Identity = 257/342 (75.15%), Postives = 290/342 (84.80%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           ME M+PKPF EG+GGSYLKWLPSDYPLLAQTNVA GRLLLR  GFA+PHYADCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVA 120
           QG DGV GFVFPNK +EVV+KL KGDLIPVP+G TSWWFN GDSDLEI+FLGETK  HV 
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 121 GDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKP 180
           GD TY++L GP GLLQGF+PEY+ K+Y L+++ET   LK+Q N LIFT+  S    LPKP
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPS--QSLPKP 180

Query: 181 HQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYV 240
           H+ SKLVYNIDAA PD     G  AVT VTES FPFIGQ+GLTA+LEKLDANA++SP Y+
Sbjct: 181 HKHSKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYI 240

Query: 241 AQPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISII 300
           A+PSDQLIYV  GSGKIQ+VGFS K FDA+VK+GQLILVP+YFA GK+AG+EGLECIS+I
Sbjct: 241 AEPSDQLIYVTKGSGKIQVVGFSSK-FDADVKIGQLILVPRYFAVGKMAGEEGLECISMI 300

Query: 301 VATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSK 343
           VAT+P VEELAGK SVLEALS  VFQ+SFNVTAEFEKLFRSK
Sbjct: 301 VATHPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSK 339

BLAST of Tan0019750 vs. ExPASy TrEMBL
Match: A0A1S3C2D5 (glutelin type-A 2-like OS=Cucumis melo OX=3656 GN=LOC103496119 PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 3.0e-145
Identity = 257/342 (75.15%), Postives = 290/342 (84.80%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           ME M+PKPF EG+GGSYLKWLPSDYPLLAQTNVA GRLLLR  GFA+PHYADCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVA 120
           QG DGV GFVFPNK +EVV+KL KGDLIPVP+G TSWWFN GDSDLEI+FLGETK  HV 
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 121 GDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKP 180
           GD TY++L GP GLLQGF+PEY+ K+Y L+++ET   LK+Q N LIFT+  S    LPKP
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPS--QSLPKP 180

Query: 181 HQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYV 240
           H+ SKLVYNIDAA PD     G  AVT VTES FPFIGQ+GLTA+LEKLDANA++SP Y+
Sbjct: 181 HKHSKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYI 240

Query: 241 AQPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISII 300
           A+PSDQLIYV  GSGKIQ+VGFS K FDA+VK+GQLILVP+YFA GK+AG+EGLECIS+I
Sbjct: 241 AEPSDQLIYVTKGSGKIQVVGFSSK-FDADVKIGQLILVPRYFAVGKMAGEEGLECISMI 300

Query: 301 VATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSK 343
           VAT+P VEELAGK SVLEALS  VFQ+SFNVTAEFEKLFRSK
Sbjct: 301 VATHPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSK 339

BLAST of Tan0019750 vs. ExPASy TrEMBL
Match: A0A5D3BLA4 (Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00380 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.4e-144
Identity = 255/340 (75.00%), Postives = 288/340 (84.71%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           ME M+PKPF EG+GGSYLKWLPSDYPLLAQTNVA GRLLLR  GFA+PHYADCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVA 120
           QG DGV GFVFPNK +EVV+KL KGDLIPVP+G TSWWFN GDSDLEI+FLGETK  HV 
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 121 GDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKP 180
           GD TY++L GP GLLQGF+PEY+ K+Y L+++ET   LK+Q N LIFT+  S    LPKP
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPS--QSLPKP 180

Query: 181 HQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYV 240
           H+ SKLVYNIDAA PD     G  AVT VTES FPFIGQ+GLTA+LEKLDANA++SP Y+
Sbjct: 181 HKHSKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYI 240

Query: 241 AQPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISII 300
           A+PSDQLIYV  GSGKIQ+VGFS K FDA+VK+GQLILVP+YFA GK+AG+EGLECIS+I
Sbjct: 241 AEPSDQLIYVTKGSGKIQVVGFSSK-FDADVKIGQLILVPRYFAVGKMAGEEGLECISMI 300

Query: 301 VATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFR 341
           VAT+P VEELAGK SVLEALS  VFQ+SFNVTAEFEKLFR
Sbjct: 301 VATHPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFR 337

BLAST of Tan0019750 vs. ExPASy TrEMBL
Match: A0A0A0L6K0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G218160 PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 4.4e-144
Identity = 257/342 (75.15%), Postives = 287/342 (83.92%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           ME M+PKPF EG+GGSY KWLPSDYPLLAQTNVA GRLLLR  GFA+PHY+DCSK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYHKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYSDCSKFGYVL 60

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVA 120
           QG DGV GFVFP K +EVV+KL KGDLIPVP G TSWWFN GDSDLEI+FLGETK  HV 
Sbjct: 61  QGEDGVTGFVFPKKCNEVVIKLKKGDLIPVPAGVTSWWFNDGDSDLEIIFLGETKRAHVP 120

Query: 121 GDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKP 180
           GD TY++L GP GLLQGF+PEY+ K+  LN++ET T LK+QPN LIFT+  S    LPKP
Sbjct: 121 GDITYFILSGPRGLLQGFTPEYVQKSCSLNQEETNTFLKSQPNVLIFTVQPS--QSLPKP 180

Query: 181 HQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYV 240
           H+ SKLVYNIDAAAPD     G  AVT VTES FPFIGQ+GLT +LEKLDANA++SP Y+
Sbjct: 181 HKYSKLVYNIDAAAPDNRAKVGDAAVTMVTESTFPFIGQTGLTPVLEKLDANAIRSPVYI 240

Query: 241 AQPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISII 300
           A+PSDQLIYV  GSGKIQ+VGFS K FDA+VK GQLILVP+YFA GKIAG+EGLECIS+I
Sbjct: 241 AEPSDQLIYVTKGSGKIQVVGFSSK-FDADVKTGQLILVPRYFAVGKIAGEEGLECISMI 300

Query: 301 VATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSK 343
           VAT+P VEELAGK SVLEALS  VFQ+SFNVTAEFEKLFRSK
Sbjct: 301 VATHPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSK 339

BLAST of Tan0019750 vs. ExPASy TrEMBL
Match: A0A6J1E9P2 (legumin J-like OS=Cucurbita moschata OX=3662 GN=LOC111430654 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.5e-139
Identity = 254/341 (74.49%), Postives = 283/341 (82.99%), Query Frame = 0

Query: 2   EPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVLQ 61
           +PM+PKPF+E + GSY KWLPS+YPLLAQ  VAAGRLLLR  GF +PHYADCSKVGYVLQ
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAQNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 62  GNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVAG 121
           G +GVAG VFP+KSDEVVV L KGDLIPVPNG +SWWFN GDSDLEI+FLGE+K  HV G
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 122 DFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKPH 181
           D +Y++L GP  LL GFSPEY+GK Y LN +ETT  LK+Q N LIF+I Q+    LPKP 
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQT--QSLPKPS 182

Query: 182 QQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYVA 241
           + SK VYNIDAAAPD     G  AVTTVTESKFPFIGQSGLTAILEKL+ANAV+SP YVA
Sbjct: 183 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLNANAVRSPVYVA 242

Query: 242 QPSDQLIYVANGSGKIQIVGFSGKFFDAEVKMGQLILVPKYFAAGKIAGDEGLECISIIV 301
           +P DQLIYVA G GKIQIVG S K  DAEVKMGQLILVPK+FA GKIAG++GLECISII 
Sbjct: 243 EPYDQLIYVAKGRGKIQIVGSSSK-IDAEVKMGQLILVPKFFAVGKIAGEDGLECISIIT 302

Query: 302 ATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSK 343
           AT+P VEELAGK SVLEALSP +FQ+SFNVTAEFEKL RSK
Sbjct: 303 ATHPVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSK 340

BLAST of Tan0019750 vs. TAIR 10
Match: AT2G28680.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.7e-68
Identity = 140/343 (40.82%), Postives = 194/343 (56.56%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           + P  PK    GDGGSY  W P + P+L   N+ A +L L + G ALP Y+D  KV YVL
Sbjct: 5   LSPRLPKKVYGGDGGSYFAWCPEELPMLRDGNIGASKLALEKYGLALPRYSDSPKVAYVL 64

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVA 120
           QG  G AG V P K +E V+ + KGD I +P G  +WWFN+ D++L ++FLGET  GH A
Sbjct: 65  QG-AGTAGIVLPEK-EEKVIAIKKGDSIALPFGVVTWWFNNEDTELVVLFLGETHKGHKA 124

Query: 121 GDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKP 180
           G FT + L G  G+  GFS E++G+A+ L+E     L+ +Q    I  +  S +   PK 
Sbjct: 125 GQFTDFYLTGSNGIFTGFSTEFVGRAWDLDETTVKKLVGSQTGNGIVKVDASLKMPEPKK 184

Query: 181 HQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYV 240
             +   V N   A  D    DG   V   T++  P +G+ G  A L ++D +++ SP + 
Sbjct: 185 GDRKGFVLNCLEAPLDVDIKDGGRVVVLNTKN-LPLVGEVGFGADLVRIDGHSMCSPGFS 244

Query: 241 AQPSDQLIYVANGSGKIQIVGFSGK-FFDAEVKMGQLILVPKYFAAGKIAGDEGLECISI 300
              + Q+ Y+  GSG++QIVG  GK   +  VK G L +VP++F   KIA  +GL   SI
Sbjct: 245 CDSALQVTYIVGGSGRVQIVGADGKRVLETHVKAGVLFIVPRFFVVSKIADSDGLSWFSI 304

Query: 301 IVATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRSK 343
           +   +P    LAG+ SV +ALSP V Q +F V  E EK FRSK
Sbjct: 305 VTTPDPIFTHLAGRTSVWKALSPEVLQAAFKVDPEVEKAFRSK 344

BLAST of Tan0019750 vs. TAIR 10
Match: AT1G07750.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 256.5 bits (654), Expect = 3.0e-68
Identity = 137/342 (40.06%), Postives = 196/342 (57.31%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           + P  PK    GDGGSY  W P + P+L Q N+ A +L L + GFA+P Y+D SKV YVL
Sbjct: 5   LTPKLPKKVYGGDGGSYSAWCPEELPMLKQGNIGAAKLALEKNGFAVPRYSDSSKVAYVL 64

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFLGETKGGHVA 120
           QG+ G AG V P K +E V+ + +GD I +P G  +WWFN+ D +L I+FLGET  GH A
Sbjct: 65  QGS-GTAGIVLPEK-EEKVIAIKQGDSIALPFGVVTWWFNNEDPELVILFLGETHKGHKA 124

Query: 121 GDFTYYLLPGPAGLLQGFSPEYIGKAYHLNEQETTTLLKTQPNPLIFTIPQSDQSQLPKP 180
           G FT + L G  G+  GFS E++G+A+ L+E     L+ +Q    I  +    +   PK 
Sbjct: 125 GQFTEFYLTGTNGIFTGFSTEFVGRAWDLDENTVKKLVGSQTGNGIVKLDAGFKMPQPKE 184

Query: 181 HQQSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYV 240
             ++  V N   A  D    DG   V   T++  P +G+ G  A L ++DA+++ SP + 
Sbjct: 185 ENRAGFVLNCLEAPLDVDIKDGGRVVVLNTKN-LPLVGEVGFGADLVRIDAHSMCSPGFS 244

Query: 241 AQPSDQLIYVANGSGKIQIVGFSGK-FFDAEVKMGQLILVPKYFAAGKIAGDEGLECISI 300
              + Q+ Y+  GSG++Q+VG  GK   +  +K G L +VP++F   KIA  +G+   SI
Sbjct: 245 CDSALQVTYIVGGSGRVQVVGGDGKRVLETHIKAGSLFIVPRFFVVSKIADADGMSWFSI 304

Query: 301 IVATNPRVEELAGKRSVLEALSPVVFQISFNVTAEFEKLFRS 342
           +   +P    LAG  SV ++LSP V Q +F V  E EK FRS
Sbjct: 305 VTTPDPIFTHLAGNTSVWKSLSPEVLQAAFKVAPEVEKSFRS 343

BLAST of Tan0019750 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-19
Identity = 86/324 (26.54%), Postives = 140/324 (43.21%), Query Frame = 0

Query: 53  CSKVGYVLQGNDGVAGFVFPNKSDE----VVVKLNKGDLIPVPNGTTSWWFNHGDSDLEI 112
           C +    ++G+ G  G   P +  E     +    +GD+     G + WW+N GDSD  I
Sbjct: 112 CPETFAEVEGSSGRGGGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYNRGDSDAVI 171

Query: 113 VFLGETKGG-----------HVAGDFTY---YLLPGPAG--LLQGFSPEYIGKAYHLNEQ 172
           V + +                +AG  T      L  P+G     GF P  I +A+ +N +
Sbjct: 172 VIVLDVTNRENQLDQVPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIAEAFKINIE 231

Query: 173 ETTTLLKTQPN---------PLIFTIPQSDQSQ-------LPKPHQQSKLVYNIDAAAPD 232
               L   + N         PL F IP   + Q       + + +  +K+  NID   P+
Sbjct: 232 TAKQLQNQKDNRGNIIRANGPLHFVIPPPREWQQDGIANGIEETYCTAKIHENID--DPE 291

Query: 233 TIPNDGTLA--VTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYVAQPSDQLIYVANGS 292
              +  T A  ++T+     P +    L A+   L +  +  P + A  +  ++YV  G 
Sbjct: 292 RSDHFSTRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTAN-AHTVLYVTGGQ 351

Query: 293 GKIQIVGFSGK-FFDAEVKMGQLILVPKYFAAGKIAGDEGLECISIIVATNPRVEELAGK 338
            KIQ+V  +G+  F+ +V  GQ+I++P+ FA  K AG+ G E IS     N  +  L+G+
Sbjct: 352 AKIQVVDDNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYINTLSGQ 411

BLAST of Tan0019750 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 90.5 bits (223), Expect = 2.8e-18
Identity = 89/400 (22.25%), Postives = 157/400 (39.25%), Query Frame = 0

Query: 1   MEPMSPKPFSEGDGGSYLKWLPSDYPLLAQTNVAAGRLLLRRCGFALPHYADCSKVGYVL 60
           +  + P    + +GG    W     P L  +  A  R ++   G  LP + +  K+ +V+
Sbjct: 35  LNALEPSQIIKSEGGRIEVW-DHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVV 94

Query: 61  QGNDGVAGFVFPNKSD------------------------EVVVKLNKGDLIPVPNGTTS 120
            G  G+ G V P  ++                        + V  L  GD I  P+G   
Sbjct: 95  HGR-GLMGRVIPGCAETFMESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTIATPSGVAQ 154

Query: 121 WWFNHGDSDLEIVFLGE--TKGGHVAGDFTYYLLPG--PAG--------------LLQGF 180
           W++N+G+  L +V   +  +    +  +   +L+ G  P G              +  GF
Sbjct: 155 WFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGF 214

Query: 181 SPEYIGKAYHLNEQETTTLLKTQPN---------PL-IFTIPQSDQSQLPKPHQ------ 240
           +PE + +A+ +N +    L   Q N         P  +   P        +PH+      
Sbjct: 215 APEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRPPLRRGEGGQQPHEIANGLE 274

Query: 241 ----QSKLVYNIDAAAPDTIPNDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPS 300
                 +   N+D  +   +       ++T+     P +    L+A+   +  NA+  P 
Sbjct: 275 ETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRLLRLSALRGSIRKNAMVLPQ 334

Query: 301 YVAQPSDQLIYVANGSGKIQIVGFSG-KFFDAEVKMGQLILVPKYFAAGKIAGDEGLECI 338
           +    ++  +YV NG   IQ+V  +G + FD E+  GQL++VP+ F+  K A  E  E I
Sbjct: 335 WNVN-ANAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQGFSVMKHAIGEQFEWI 394

BLAST of Tan0019750 vs. TAIR 10
Match: AT4G28520.1 (cruciferin 3 )

HSP 1 Score: 70.5 bits (171), Expect = 3.0e-12
Identity = 76/316 (24.05%), Postives = 126/316 (39.87%), Query Frame = 0

Query: 61  QGNDGVAGFVFPNKSDEVVVKLNKGDLIPVPNGTTSWWFNHGDSDLEIVFL--------- 120
           QG  G  GF       + V  + +GD+     G+  W +N G+  L I+ L         
Sbjct: 181 QGQQGQQGF---RDMHQKVEHVRRGDVFANTPGSAHWIYNSGEQPLVIIALLDIANYQNQ 240

Query: 121 --GETKGGHVAGDFTYYLLPG------PAGLLQGFSPEYIGKAYHL---------NEQET 180
                +  H+AG+       G         L  GF  + I +A  +         N+Q++
Sbjct: 241 LDRNPRVFHLAGNNQQGGFGGSQQQQEQKNLWSGFDAQVIAQALKIDVQLAQQLQNQQDS 300

Query: 181 TTLLKTQPNPLIFTIP------QSDQSQLPKPHQQSKLV---------YNIDAAAPDTIP 240
              +     P     P      +S++ + P+  Q + L           NID  A   + 
Sbjct: 301 RGNIVRVKGPFQVVRPPLRQPYESEEWRHPRSPQGNGLEETICSMRSHENIDDPARADVY 360

Query: 241 NDGTLAVTTVTESKFPFIGQSGLTAILEKLDANAVQSPSYVAQPSDQLIYVANGSGKIQI 300
                 VT+V     P +    L+A    L  NA+  P Y    +++++Y   G G+IQ+
Sbjct: 361 KPSLGRVTSVNSYTLPILEYVRLSATRGVLQGNAMVLPKY-NMNANEILYCTGGQGRIQV 420

Query: 301 VGFSGK-FFDAEVKMGQLILVPKYFAAGKIAGDEGLECISIIVATNPRVEELAGKRSVLE 335
           V  +G+   D +V+ GQL+++P+ FA    +     E IS     N  +  LAG+ S+L 
Sbjct: 421 VNDNGQNVLDQQVQKGQLVVIPQGFAYVVQSHGNKFEWISFKTNENAMISTLAGRTSLLR 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZWA91.6e-1826.5412S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1[more]
P154564.0e-1722.2512S seed storage protein CRB OS=Arabidopsis thaliana OX=3702 GN=CRB PE=1 SV=2[more]
A0A222NNM93.4e-1621.82Cocosin 1 OS=Cocos nucifera OX=13894 GN=COS-1 PE=1 SV=1[more]
P077289.8e-1621.39Glutelin type-A 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA1 PE=1 SV=2[more]
P137443.7e-1522.0411S globulin subunit beta OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_008456076.16.3e-14575.15PREDICTED: glutelin type-A 2-like [Cucumis melo] >KAA0039043.1 glutelin type-A 2... [more]
TYJ99759.16.9e-14475.00glutelin type-A 2-like [Cucumis melo var. makuwa][more]
XP_004151504.19.1e-14475.15legumin J [Cucumis sativus] >KGN57580.1 hypothetical protein Csa_009841 [Cucumis... [more]
XP_022922755.15.2e-13974.49legumin J-like [Cucurbita moschata][more]
KAG6576976.15.2e-13974.4912S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A5A7T7U83.0e-14575.15Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A1S3C2D53.0e-14575.15glutelin type-A 2-like OS=Cucumis melo OX=3656 GN=LOC103496119 PE=3 SV=1[more]
A0A5D3BLA43.4e-14475.00Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A0A0L6K04.4e-14475.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G218160 PE=4 SV=1[more]
A0A6J1E9P22.5e-13974.49legumin J-like OS=Cucurbita moschata OX=3662 GN=LOC111430654 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G28680.11.7e-6840.82RmlC-like cupins superfamily protein [more]
AT1G07750.13.0e-6840.06RmlC-like cupins superfamily protein [more]
AT1G03890.11.1e-1926.54RmlC-like cupins superfamily protein [more]
AT1G03880.12.8e-1822.25cruciferin 2 [more]
AT4G28520.13.0e-1224.05cruciferin 3 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 188..337
e-value: 3.3E-13
score: 59.9
coord: 5..155
e-value: 4.5E-23
score: 92.7
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 189..335
e-value: 8.6E-14
score: 51.4
coord: 5..153
e-value: 1.4E-17
score: 63.7
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 194..343
e-value: 2.4E-35
score: 123.2
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 4..176
e-value: 4.1E-29
score: 103.1
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 1..342
NoneNo IPR availablePANTHERPTHR31189:SF4511S GLOBULIN SEED STORAGE PROTEIN 2-LIKEcoord: 1..342
NoneNo IPR availableCDDcd02242cupin_11S_legumin_Ncoord: 3..163
e-value: 3.16724E-52
score: 170.073
NoneNo IPR availableCDDcd02243cupin_11S_legumin_Ccoord: 206..342
e-value: 1.81682E-53
score: 171.118
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 4..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0019750.1Tan0019750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0045735 nutrient reservoir activity