Tan0019462 (gene) Snake gourd v1

BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match: Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 950.3 bits (2455), Expect = 1.6e-275
Identity = 479/877 (54.62%), Postives = 626/877 (71.38%), Query Frame = 0

Query: 14  CVCNCGITESFKLAVSDHHT-YGHKIKYQSGKMDEARMVARGESKVGFLKSSHRNLSKAY 73
           C  N GIT ++K   +   T   +KIK  S  +   R    G+     L+S     SK  
Sbjct: 13  CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQS--YQFSKIC 72

Query: 74  ATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRSGFTVYVEMSSLQ 133
           A S  S+E          ++ +  KV +Y FRT+ G LV V VEK R  +++ V +SSL+
Sbjct: 73  A-SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLE 132

Query: 134 LTSVD-GKPMLSWGVYRSDSAIVTP-NFQSSLPDGTIGTTATPFVNTSEGNFAVELEFDA 193
           L+  D  + ++ WGVYRSDS+   P +F++S  D    TT T FV +S     + LEFD 
Sbjct: 133 LSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDG 192

Query: 194 KHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSIS-GDGSINFS 253
           K  PFYLSF LK          E+ +HR T FC+PVGF  G+P PLGLS    D S NFS
Sbjct: 193 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFS 252

Query: 254 IFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVSYGYRCKGSK 313
            FS ++ +VVLCLY+DS ++KP LELDLDPY+NR+G++WHAS ++  +FV YGYRCK + 
Sbjct: 253 FFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETA 312

Query: 314 FHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFDWDREVRPNL 373
                 +VE   I++DPYA ++  S+          + ++LG + K P+FDW  +V PN+
Sbjct: 313 HSKEDVDVEGEPIVLDPYATVVGKSV----------SQKYLGSLSKSPSFDWGEDVSPNI 372

Query: 374 PMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLIEPIFPFDEK 433
           P+EKL VYRLNVK FT  +SS+LP+++AGTFSG+ +K+ H  TLG NAVL+EPIF F E+
Sbjct: 373 PLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQ 432

Query: 434 EGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTHTAVDGALQG 493
           +GPYFP HFFSP +IYGPS    SA+NSMK MVK+LH+ G+EV+LEVVFTHTA  GAL+G
Sbjct: 433 KGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRG 492

Query: 494 IDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGH 553
           IDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFH+DGFCFINASSLLRG 
Sbjct: 493 IDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGV 552

Query: 554 HGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIR 613
           HGE LSRPPLVEAIAFDPLL++TK+IAD WDP E +  E RFPHWKRWAE+N+++C ++R
Sbjct: 553 HGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVR 612

Query: 614 DFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELS 673
           +F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN+GL LVD+VSFS  +LASELS
Sbjct: 613 NFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELS 672

Query: 674 WNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGSVAFNDRRSF 733
           WNCGEEG T+ + VL++RLKQIRNF+F+ ++SLGVPVL+MGDECG S+ GS     R+ F
Sbjct: 673 WNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPF 732

Query: 734 DWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSPPQWEDPSCK 793
           DW+LL + FGTQ TQFI+F++S RA+R D++Q R+FLK ENI WY N+Q+ P+WEDP+ K
Sbjct: 733 DWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASK 792

Query: 794 FLAVILR--ADKEENGSITEDDKPRS-DIFMAFNASDQSESVALPEPSEGTSWFRLIDTA 853
           FLA+ ++  +++EE  S+ E ++P+S D+F+ FNASD  ESV LP   +G+ W RL+DTA
Sbjct: 793 FLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTA 852

Query: 854 LSFPGFFSSDGEPV----PATGSATYEMQAHSCALFE 880
           L FPGFFS +GE V    P      YEM+ +SC LFE
Sbjct: 853 LPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match: Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 1.6e-219
Identity = 375/718 (52.23%), Postives = 502/718 (69.92%), Query Frame = 0

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
           + A EL FD    PFYLSFLL       +  +EIR+HRKTSF VPVG G G P+PLG+SI
Sbjct: 103 SLAAELSFDPTRAPFYLSFLL-----TDASGAEIRTHRKTSFRVPVGVGPGSPAPLGMSI 162

Query: 241 SGDGSINFSIFSSTAESVVLCLY----NDSKSEKPVLELDLDPYINRSGNIWHASFESAL 300
           SGDG++NF+++S  A +V L LY         ++P LE+DLDPYI+R+GN+WH S  S  
Sbjct: 163 SGDGAVNFAVYSKNANAVSLYLYAAAVGGGGGDEPALEIDLDPYIHRTGNVWHVSLASVD 222

Query: 301 NFVSYGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTS---RFLGQV 360
            +VSY + C G           + R L+DPYAK++   +        G T+   R    +
Sbjct: 223 GYVSYAFCCGG-----------IRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASL 282

Query: 361 CKVPAFDWDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITL 420
              P+++W R+  P LP+EKL VYR NV  FT D+SS LP D AGTF+GL+ K+ HF +L
Sbjct: 283 AIAPSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSL 342

Query: 421 GVNAVLIEPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVM 480
           GVNA+L+EP+FPF + +GPYFP HFFSP N+Y     S+SAI SMK+MV+ +H NG+EV+
Sbjct: 343 GVNAILLEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVL 402

Query: 481 LEVVFTHTAVDG------ALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRY 540
           LEVVFTHTA         +++GID+SSYY  N +   +  S LNCN+P+ Q+L+LDSLR+
Sbjct: 403 LEVVFTHTAEGESECQTISMRGIDNSSYYIANGIAGCKA-SILNCNHPVTQKLILDSLRH 462

Query: 541 WVTEFHIDGFCFINASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILN 600
           WV +FH+DGFCFINA  L+RG  GE LSRPPL+EAI FDP+LS TKIIAD W P +    
Sbjct: 463 WVLDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNV 522

Query: 601 ETRFPHWKRWAEVNSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIAR 660
           +  FPHWKRWAEVN++F  D+R F + E L+S LATRLCGSGD+FS  RGPAFSFN ++R
Sbjct: 523 QFPFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSR 582

Query: 661 NAGLPLVDLVSFSSSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVL 720
           N+GL LVDLVSFS+  L SE SWNCGEEGP+ N+ VL+ RL+QIRNF+F+LFVSLGVPVL
Sbjct: 583 NSGLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVL 642

Query: 721 HMGDECGQSSGGSVAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLK 780
           +MGDECG S+ GSV++ DR   +W  +KT F  + T FI+FL++ R++R D++Q R FLK
Sbjct: 643 NMGDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLK 702

Query: 781 GENIDWYGNNQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSES 840
            ENI WYG++   P W+DP+  FL + + A+ +E  +    D  R D+++ FNA+++S S
Sbjct: 703 LENIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANEESVS 762

Query: 841 VALPEPSEGTSWFRLIDTALSFPGFFSSDGEP--VPATGSATYEMQAHSCALFEAKSS 884
            ALP  +EG+ W RL+DT+L+FPGFF+++  P      G ++Y ++AH+C LFE+KS+
Sbjct: 763 AALPALAEGSVWLRLVDTSLAFPGFFATESNPKVQQVPGLSSYHVEAHTCVLFESKSA 798

BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 383.3 bits (983), Expect = 7.7e-105
Identity = 251/705 (35.60%), Postives = 360/705 (51.06%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSKSEKPVLELDLDPYINRSG 290
           G  SPLG+S   D  INF++FS  A SV LCL       D   +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFES-ALNFVSYGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLG 350
           + WH   E   LN V YGYR  G          + S +L+DPYAK++      + G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207

Query: 351 STSRFLGQV-CKVPAFDWDREVR-PNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSG 410
             ++F G    +   FDW  + + PN+P + L +Y +NV+ FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHFITLGVNAVLIEPIFPFDEKE--------------GPYFPSHFFSPTNIYGPS 470
             +K+ H   LG+NAV + P+F FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 LGS-ISAINSMKEMVKELHANGVEVMLEVVFTHT-------AVDGALQGIDDSSYYFV-- 530
            G  I A    KEMVK LH+ G+EV+L+VV+ HT           + +GID+  YY +  
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 -NRVVNLEG-KSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGHHGELLSR 590
            N+++N  G  + LNCN+P+V +L+LDSLR+WVTE+H+DGF F  AS L R   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIRDFFRGE- 650
           PPL+ AIA D +LS+ KIIA+ WD     L   +FP+W RWAE N  + +D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLYL-VGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLVSSLATRLCGSGDVFS-DGRGPAFSFNFIARNAGLPLVDLVSFS-----------SSK 710
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           +  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECG-------QS 770
                SWNCG EG T +A +   R +Q++NF   L +S G P++ MGDE G        S
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 SGGSVAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGN 830
            G   + N+ +  + D  K       ++ I F  S    + +     NFL    I W+ +
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHE-----NFLTQGEITWHED 687

Query: 831 NQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEG 882
           N     W++   KFLA  L            D     DI++AFNA D      +P+P  G
Sbjct: 688 N-----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIPQPPPG 747

BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 5.5e-103
Identity = 255/730 (34.93%), Postives = 373/730 (51.10%), Query Frame = 0

Query: 208 GSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSISGDGSINFSIFSSTAESVVLCLY---- 267
           G   +E R    +   +P  +  G   PLG+S   +G +NF++FS  A SV+LCL     
Sbjct: 80  GRSMTEERGCTMSDTEMPFKYSSGKAFPLGVS-QVEGGLNFALFSQHASSVILCLKLPGR 139

Query: 268 -NDSKSEKPVLELDLDPYINRSGNIWHASFES-ALNFVSYGYRCKGSKFHANQDEVEVSR 327
             + +    V+E  LD   N++G+IWH   E    + V YGYR  G +        + S 
Sbjct: 140 GTEDEKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSST 199

Query: 328 ILVDPYAKILTASIPKTCGQGLGSTSRFLGQV-CKVPAFDWDREVR-PNLPMEKLFVYRL 387
           +L+DPYAK+++    K  G     +S+  G        FDW  + R PNLP   L +Y +
Sbjct: 200 VLLDPYAKLVSGR--KYFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEM 259

Query: 388 NVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLIEPIFPFDEKEGPYFPS--- 447
           NV+ FT D+SS L +   G++ GL  K+ H + LGVNAV + P+F +DE E   +P+   
Sbjct: 260 NVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRD 319

Query: 448 -----------HFFSPTNIYGPSLGS-ISAINSMKEMVKELHANGVEVMLEVVFTHT--A 507
                      +FF+P + Y  + G  ++A   +K+MVKELH  G+EV+L+VV+ HT  A
Sbjct: 320 HMVNTWGYSTINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEA 379

Query: 508 VDG-----ALQGIDDSSYYFVN-----RVVNLEG-KSALNCNYPIVQQLLLDSLRYWVTE 567
            D      + +GID+  YY ++      ++N  G  + LNCN+P+V++L+LDSLR+WV E
Sbjct: 380 DDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEE 439

Query: 568 FHIDGFCFINASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRF 627
           +HIDGF F  AS L RG  G  L  PPL++ IA D +LS+ KIIA+ WD     L   RF
Sbjct: 440 YHIDGFRFDLASVLCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLYL-VGRF 499

Query: 628 PHWKRWAEVNSKFCNDIRDFFRGE-GLVSSLATRLCGSGDVFS-DGRGPAFSFNFIARNA 687
           P+W RWAE N K+ +D+R F +G+ G+    ATR+ GS D++  + R P    NF+  + 
Sbjct: 500 PNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHD 559

Query: 688 GLPLVDLVSFS-----------SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVL 747
           G  L DLVS++                   SWNCG EG T++  VL  R +Q++NF   L
Sbjct: 560 GFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVAL 619

Query: 748 FVSLGVPVLHMGDECG-------QSSGGSVAFNDRRSFDWDLLKTGFGTQTTQFITFLSS 807
            +S G P++ MGDE G        S G     N   +F W+ L+        +F + +  
Sbjct: 620 MISQGTPMMLMGDEYGHTRYGNNNSYGHDTCIN---NFQWEQLEQRRDGH-FRFFSEMIK 679

Query: 808 FRAKRFDLYQSRNFLKGENIDWYGNNQSPPQWEDPSCKFLAVILRADKEENGSITEDDKP 867
           FR     L + R FL   ++ W+ +      WE+   KFLA  +            D   
Sbjct: 680 FRHSNPILRRDR-FLNKNDVTWHED-----CWENQESKFLAFTV-----------HDHNS 739

Query: 868 RSDIFMAFNASDQSESVALPEPSEGTSWFRLIDTALSFPGFFSSDGEPVPATGSATYEMQ 882
             DI++AFNA D      +P P     W R++DT L  P     +G  VP TG   Y + 
Sbjct: 740 GGDIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEG--VPFTG-PKYRIA 781

BLAST of Tan0019462 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 4.2e-95
Identity = 231/694 (33.29%), Postives = 348/694 (50.14%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY--NDSKSEKPVLELDLDPYINRSGNIW 290
           G P+PLG + + DG +NF+++S+ A +  LCL+  +D ++++   E+ LDP  NR+GN+W
Sbjct: 99  GMPAPLG-ATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVW 158

Query: 291 HASFESALNFVSYGYRCKGSKFHANQDEVEVSRILVDPYAK-ILTASIPKTCGQGLGSTS 350
           H   E  L+ + YGYR  G          +VS ++VDPYAK +++       G G     
Sbjct: 159 HVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWP 218

Query: 351 RFLGQV-CKVPAFDWDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKK 410
           +  G +      FDW  ++    P + L +Y ++++ FT   SS +  +  GT+ G   K
Sbjct: 219 QMAGMIPLPYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNV--EHPGTYIGAISK 278

Query: 411 LLHFITLGVNAVLIEPIFPFDEKE----------GPYFPSHFFSPTNIYGPSLGSI---- 470
           L +   LGVN V + P   F+E E            Y   +FFSP   Y  S G I    
Sbjct: 279 LDYLKELGVNCVELMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRY--SSGGIRNCG 338

Query: 471 -SAINSMKEMVKELHANGVEVMLEVVFTHTAVDG------ALQGIDDSSYYFV---NRVV 530
             AIN  K  V+E H  G+EV+++VVF HTA         + +GID+S+YY +       
Sbjct: 339 RDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFY 398

Query: 531 NLEG-KSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRG------------- 590
           N  G  +  NCN+P+V++ ++D LRYWVTE H+DGF F  AS + RG             
Sbjct: 399 NYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSP 458

Query: 591 ------HHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNS 650
                   G  L+ PPL++ I+ DP+L   K+IA+ WD    +    +FPHWK W+E N 
Sbjct: 459 VEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWD-AGGLYQVGQFPHWKIWSEWNG 518

Query: 651 KFCNDIRDFFRG-EGLVSSLATRLCGSGDVF-SDGRGPAFSFNFIARNAGLPLVDLVSFS 710
           K+ + +R F +G +G     A  LCGS  ++ + GR P  S NF+  + G  L DLV+++
Sbjct: 519 KYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYN 578

Query: 711 SSKLAS-----------ELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHM 770
               +S            LSWNCGEEG  +   V   R +Q+RNF   L VS GVP+ +M
Sbjct: 579 KKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYM 638

Query: 771 GDECGQSSGG--SVAFNDR--RSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNF 830
           GDE G + GG  +   +D     F WD  K    +   +F + ++ FR K+ +     +F
Sbjct: 639 GDEYGHTKGGNNNTYCHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADF 698

Query: 831 LKGENIDWYGNNQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQS 860
              + + W+G+    P W + S +F+A             +  D+ + +I++AFNAS   
Sbjct: 699 PTAQRLHWHGHQPGKPDWSETS-RFVA------------FSTKDETKGEIYVAFNASHLP 758

BLAST of Tan0019462 vs. NCBI nr
Match: XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1564.7 bits (4050), Expect = 0.0e+00
Identity = 770/885 (87.01%), Postives = 808/885 (91.30%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT LSSLVIQPFCV N G+TESFKLAV DHHTYG KIKY SGKMDEARM+A GES+VG 
Sbjct: 1   MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
            KSSHRNLSK YATSGI V KSGQRFG  GKSKE  KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61  TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
           GF V VE+SS+QL S++   +LSWGVYRSDSAIVTPNFQSSLPD TIG T TPFV TS G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            FAVELEFDAKH PFYLSFLLKYPV V SG+SEIRSHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDS SEKPVLELDLD YINRSG+IWHASFESA NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGYRCKGSKFH NQD VEV+RI+VDPYAKILTAS+ KT GQGLG   +FLGQ+CKVPAFD
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLG-LPKFLGQICKVPAFD 360

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EV PNLPMEKLF YRLNVK FTVDKSSQLP DIAGTFSGLTKKLLHF  LGVNAVL+
Sbjct: 361 WDGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIF FDEKEG YFP HFFSP N YGPS  SISAINSMK MVK+LHANGVEV+LEVVFTH
Sbjct: 421 EPIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTH 480

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           TA+DGALQGIDDSSYYFVNRV NLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NASSLLRGHHGE LSR P VEAIAFDPLLSKTK++ADFWDPQE +  ET FPHWKRWAEV
Sbjct: 541 NASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEV 600

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKFCND+RDFFRGEGL+SSLATRLCGSGDVFS+GRGPAFSFNFI RN GLPLVDLVSFS
Sbjct: 601 NSKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           +SKLASELSWNCGEEGPTSN EVLEKRLKQIRNFIFV+FVSLGVPVL+MGDECGQS+GGS
Sbjct: 661 NSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVVFVSLGVPVLNMGDECGQSAGGS 720

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           VAFNDRRSF+WDLLKT FGTQTTQFITFLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWEDPSCKFLAV+LRADKEENGSITE+ KPRSDIFMAFNASDQSE+VALPEPSEGTSWF
Sbjct: 781 PQWEDPSCKFLAVMLRADKEENGSITENAKPRSDIFMAFNASDQSETVALPEPSEGTSWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
           RL+DTALSFPGFFSSDGE VP TGS TYEMQAHSCALFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEQVPTTGSVTYEMQAHSCALFEAKSFND 884

BLAST of Tan0019462 vs. NCBI nr
Match: XP_022150231.1 (isoamylase 2, chloroplastic [Momordica charantia])

HSP 1 Score: 1532.3 bits (3966), Expect = 0.0e+00
Identity = 749/885 (84.63%), Postives = 799/885 (90.28%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT  SSLV+QP+ V N G+TE+F+LA SDHHT+GH IK+QSGK DE RM+A GES+ GF
Sbjct: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
            KSS  NLSKAYA+ GISVEK+G RFGISGKSKE+ KVATYLFRTE GDLVNV V KT S
Sbjct: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
           GF V +E+SS+QL +++  PMLSWGVYRSDSA+VTPN QSSLPD TIGT  TPFVNTSEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            FAVELEFDAKH+PFYLSFLLK+PV V SG+SEIRSHRKTSFCVPVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGS+NFSIFSS+AESVVLCLYNDS SEKPV+ELDLD YINRSGNIWH SFES  NFVS
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGY CKGSK H  QD VE+SRILVDPYAK+LT S  KT G GLG  S+ LGQ+CKVPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EVRPNL MEKLF YRLNVK FTVDKSSQL TDIAGTF+GLTKKLLHF  LGVNAVL+
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIFPFDEKEGPYFPSHFFSPTNIYGPS GSISAINSMKEMVKELHANG+EVMLEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           TA DG LQGIDDSSYYF NRV NLEGK+ALNCNYP+VQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NASSLLRG+ GELLSRPPLVEAIAFDPLLSKTKIIADFWDP ESI  ETRFPHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           N KFC+D+RDFFRGEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPTSN EVLEKRLK IRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           +AF DRR FDWDLLKTGFGTQTTQFITF SSFRA+RFDL+QSRNFLKGENIDWYGNN SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWEDPSCKFLAV+LRADKEENG   E+ +P+SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
           RLIDTALSFPGFFSSDGE VP   S TYEMQAHSCALFEAKS +D
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891

BLAST of Tan0019462 vs. NCBI nr
Match: XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 748/884 (84.62%), Postives = 798/884 (90.27%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT LSSLVIQPFCV NCG+TES KLAVSDH TYG K KYQSGKMDEARM+A GESKVG 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
           +KSSHRNLSKAY TSGI V KSGQR GI GKSKE+ KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
            FTV +E+SS+QL S D   +LSW VYRSDSA VT NFQSS PDGT G T TPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            F+VELEFDAKH PFYLSF+LK+P+ V SG+SEIRSHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE A NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGY+CKG K H NQD +EVSRI+VDPYAKIL  SIPK+  QGLG   +FLGQ+  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EVRPNLPMEKLFVYRLNVK FT+DKSSQLP DIAGTFSGLTKKLLH   LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIF FDEKEGPYFP HFFSPTN YGPS  SISAINSMKEMVKELHANGVEV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           T+VDGALQGIDDSSYYF NRV NLE +SALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE +  ETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           +S LASELSWNCGEEGPT+  +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           VAFNDRRSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWED SCKFLAV+LRADKEEN SITE+ K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSN 885
           R++DTAL FPGFFSSDGE VP TGS TYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Tan0019462 vs. NCBI nr
Match: XP_022953509.1 (isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata] >XP_022953510.1 isoamylase 2, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 751/885 (84.86%), Postives = 800/885 (90.40%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT LSSL IQPFC  N G TESFKLAVSD+HTYG K+ YQSGK+DEARM A GESKVGF
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
            KS HRN+ +AYA S ISV KSGQ+FG  GK++E  KVATYLFRTEFGDLV  FV K  S
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
           GF   +E+SSL+L S+D   +LSWGVYRS+SAIVTPNFQS LP+ TIG T TPFV TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            FAVELEFDAKH PFYLSFLLKYPV V SG+SEI+SHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDS SEKPVLELDLDPYINRSGNIWHASFE A NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGYRC+GSKFH NQDE EVSRILVDPYAKILTASIPKT GQGLG  S+FLGQ+C VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EVRPNLPMEKLF+YRLNVKHFTVDKSSQLP D AGTFSGLT+KLLHF  LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIFPFDEK+GPYFP HFFSPTNIY PS  SIS++NS+KEMVKELHANGVEV+LEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           TA+DGALQGIDDS YYFVNRV N EGKSALNCN+PIVQQLLLDSLR+WVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NASSLL+GHHGELLSRPP VEAIAFDPLLSKTKIIADFWDPQES+  ETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKF NDI++FFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPTSN EVLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           VAF DR+SF+WD LKT FGTQTTQFITFLSSFR++RFDL+QSRNFLK ENIDW+ NNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           P WEDPSCKFLAV+LRADKEE+GSITE+ KP+SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
           RL+DTALSFPGFFSSDGEPVP T   TY MQAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884

BLAST of Tan0019462 vs. NCBI nr
Match: KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 747/884 (84.50%), Postives = 797/884 (90.16%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT LSSLVIQPFCV NCG+TES KLAVSDH TYG K KYQSGKMDEARM+A GESKVG 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
           +KSSHRNLSKAY TSGISV KSGQR GI GKSKE+ KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
            FTV +E+SS+QL S D   +LSW VYRSDSA VT NFQSS PDGT G T TPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            F+VELEFDAKH PFYLSF+LK+P+ V SG+SEIRSHRKTSF VPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE   NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGY+CKG K H NQD +EVSRI+VDPYAKIL  SIPK+  QGLG   +FLGQ+  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EVRPNLPMEKLFVYRLNVK FT+DKSSQLP DIAGTFSGLTKKLLH   LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIF FDEKEGPYFP HFFSPTN YGPS  SISAINSMKEMVKELHANGVEV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           T+VDGALQGIDDSSYYF NRV NLE +SALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE +  ETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           +S LASELSWNCGEEGPT+  +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           VAFNDRRSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWED SCKFLAV+LRADKEEN SITE+ K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSN 885
           R++DTAL FPGFFSSDGE VP TGS TYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Tan0019462 vs. ExPASy TrEMBL
Match: A0A6J1DAX4 (isoamylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018448 PE=4 SV=1)

HSP 1 Score: 1532.3 bits (3966), Expect = 0.0e+00
Identity = 749/885 (84.63%), Postives = 799/885 (90.28%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT  SSLV+QP+ V N G+TE+F+LA SDHHT+GH IK+QSGK DE RM+A GES+ GF
Sbjct: 7   MATFPSSLVVQPYNVHNFGVTEAFRLAASDHHTHGHMIKHQSGKRDEVRMLAHGESRAGF 66

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
            KSS  NLSKAYA+ GISVEK+G RFGISGKSKE+ KVATYLFRTE GDLVNV V KT S
Sbjct: 67  AKSSRYNLSKAYASFGISVEKNGWRFGISGKSKEQEKVATYLFRTECGDLVNVLVRKTGS 126

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
           GF V +E+SS+QL +++  PMLSWGVYRSDSA+VTPN QSSLPD TIGT  TPFVNTSEG
Sbjct: 127 GFMVSIELSSVQLANIEETPMLSWGVYRSDSALVTPNIQSSLPDDTIGTIETPFVNTSEG 186

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            FAVELEFDAKH+PFYLSFLLK+PV V SG+SEIRSHRKTSFCVPVGFGRGYPSPLG+SI
Sbjct: 187 KFAVELEFDAKHVPFYLSFLLKHPVGVDSGSSEIRSHRKTSFCVPVGFGRGYPSPLGVSI 246

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGS+NFSIFSS+AESVVLCLYNDS SEKPV+ELDLD YINRSGNIWH SFES  NFVS
Sbjct: 247 SGDGSMNFSIFSSSAESVVLCLYNDSTSEKPVIELDLDRYINRSGNIWHVSFESVSNFVS 306

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGY CKGSK H  QD VE+SRILVDPYAK+LT S  KT G GLG  S+ LGQ+CKVPAFD
Sbjct: 307 YGYLCKGSKSHKKQDGVEISRILVDPYAKVLTTSSHKTHGHGLGLPSKLLGQICKVPAFD 366

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EVRPNL MEKLF YRLNVK FTVDKSSQL TDIAGTF+GLTKKLLHF  LGVNAVL+
Sbjct: 367 WDGEVRPNLAMEKLFTYRLNVKRFTVDKSSQLTTDIAGTFAGLTKKLLHFKNLGVNAVLL 426

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIFPFDEKEGPYFPSHFFSPTNIYGPS GSISAINSMKEMVKELHANG+EVMLEVVFT+
Sbjct: 427 EPIFPFDEKEGPYFPSHFFSPTNIYGPSGGSISAINSMKEMVKELHANGIEVMLEVVFTN 486

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           TA DG LQGIDDSSYYF NRV NLEGK+ALNCNYP+VQQLLLDSLRYWVTEFHIDGFCFI
Sbjct: 487 TAEDGTLQGIDDSSYYFTNRVANLEGKNALNCNYPVVQQLLLDSLRYWVTEFHIDGFCFI 546

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NASSLLRG+ GELLSRPPLVEAIAFDPLLSKTKIIADFWDP ESI  ETRFPHWKRWAEV
Sbjct: 547 NASSLLRGYRGELLSRPPLVEAIAFDPLLSKTKIIADFWDPHESISKETRFPHWKRWAEV 606

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           N KFC+D+RDFFRGEGLVSSLATRLCGSGD+FSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 607 NYKFCSDVRDFFRGEGLVSSLATRLCGSGDIFSDGRGPAFSFNFIARNFGLPLVDLVSFS 666

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPTSN EVLEKRLK IRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 667 SSKLASELSWNCGEEGPTSNIEVLEKRLKLIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 726

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           +AF DRR FDWDLLKTGFGTQTTQFITF SSFRA+RFDL+QSRNFLKGENIDWYGNN SP
Sbjct: 727 IAFKDRRPFDWDLLKTGFGTQTTQFITFFSSFRARRFDLFQSRNFLKGENIDWYGNNLSP 786

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWEDPSCKFLAV+LRADKEENG   E+ +P+SDIFMAFNASDQS +VALPEPSEGTSWF
Sbjct: 787 PQWEDPSCKFLAVMLRADKEENGLSAENPEPQSDIFMAFNASDQSINVALPEPSEGTSWF 846

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
           RLIDTALSFPGFFSSDGE VP   S TYEMQAHSCALFEAKS +D
Sbjct: 847 RLIDTALSFPGFFSSDGELVPTVESVTYEMQAHSCALFEAKSYSD 891

BLAST of Tan0019462 vs. ExPASy TrEMBL
Match: A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1527.7 bits (3954), Expect = 0.0e+00
Identity = 748/884 (84.62%), Postives = 798/884 (90.27%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT LSSLVIQPFCV NCG+TES KLAVSDH TYG K KYQSGKMDEARM+A GESKVG 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
           +KSSHRNLSKAY TSGI V KSGQR GI GKSKE+ KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
            FTV +E+SS+QL S D   +LSW VYRSDSA VT NFQSS PDGT G T TPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            F+VELEFDAKH PFYLSF+LK+P+ V SG+SEIRSHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE A NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGY+CKG K H NQD +EVSRI+VDPYAKIL  SIPK+  QGLG   +FLGQ+  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EVRPNLPMEKLFVYRLNVK FT+DKSSQLP DIAGTFSGLTKKLLH   LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIF FDEKEGPYFP HFFSPTN YGPS  SISAINSMKEMVKELHANGVEV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           T+VDGALQGIDDSSYYF NRV NLE +SALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE +  ETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           +S LASELSWNCGEEGPT+  +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           VAFNDRRSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWED SCKFLAV+LRADKEEN SITE+ K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSN 885
           R++DTAL FPGFFSSDGE VP TGS TYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Tan0019462 vs. ExPASy TrEMBL
Match: A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 751/885 (84.86%), Postives = 800/885 (90.40%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT LSSL IQPFC  N G TESFKLAVSD+HTYG K+ YQSGK+DEARM A GESKVGF
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
            KS HRN+ +AYA S ISV KSGQ+FG  GK++E  KVATYLFRTEFGDLV  FV K  S
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
           GF   +E+SSL+L S+D   +LSWGVYRS+SAIVTPNFQS LP+ TIG T TPFV TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            FAVELEFDAKH PFYLSFLLKYPV V SG+SEI+SHRKTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDS SEKPVLELDLDPYINRSGNIWHASFE A NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGYRC+GSKFH NQDE EVSRILVDPYAKILTASIPKT GQGLG  S+FLGQ+C VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EVRPNLPMEKLF+YRLNVKHFTVDKSSQLP D AGTFSGLT+KLLHF  LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIFPFDEK+GPYFP HFFSPTNIY PS  SIS++NS+KEMVKELHANGVEV+LEVVFTH
Sbjct: 421 EPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTH 480

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           TA+DGALQGIDDS YYFVNRV N EGKSALNCN+PIVQQLLLDSLR+WVTEFHIDGFCFI
Sbjct: 481 TAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFI 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NASSLL+GHHGELLSRPP VEAIAFDPLLSKTKIIADFWDPQES+  ETRFPHWKRWAE+
Sbjct: 541 NASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAEL 600

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKF NDI++FFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           SSKLASELSWNCGEEGPTSN EVLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 SSKLASELSWNCGEEGPTSNIEVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           VAF DR+SF+WD LKT FGTQTTQFITFLSSFR++RFDL+QSRNFLK ENIDW+ NNQSP
Sbjct: 721 VAFKDRKSFNWDSLKTDFGTQTTQFITFLSSFRSRRFDLFQSRNFLKVENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           P WEDPSCKFLAV+LRADKEE+GSITE+ KP+SDIFMAFNASDQS SVALPEPSEGT+WF
Sbjct: 781 PDWEDPSCKFLAVMLRADKEESGSITENPKPQSDIFMAFNASDQSVSVALPEPSEGTNWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
           RL+DTALSFPGFFSSDGEPVP T   TY MQAHSC LFEAKS ND
Sbjct: 841 RLVDTALSFPGFFSSDGEPVPTTSPVTYGMQAHSCTLFEAKSFND 884

BLAST of Tan0019462 vs. ExPASy TrEMBL
Match: A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)

HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 747/884 (84.50%), Postives = 797/884 (90.16%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT LSSLVIQPFCV NCG+TES KLAVSDH TYG K KYQSGKMDEARM+A GESKVG 
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
           +KSSHRNLSKAY TSGISV KSGQR GI GKSKE+ KVATYLFRTEFGDLVNVFV KT S
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
            FTV +E+SS+QL S D   +LSW VYRSDSA VT NFQSS PDGT G T TPFV TSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            F+VELEFDAKH PFYLSF+LK+P+ V SG+SEIRSHRKTSF VPVGFGRGYPSP GLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGSINFSIFSSTAESVVLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE   NFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGY+CKG K H NQD +EVSRI+VDPYAKIL  SIPK+  QGLG   +FLGQ+  VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EVRPNLPMEKLFVYRLNVK FT+DKSSQLP DIAGTFSGLTKKLLH   LGVNAVL+
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIF FDEKEGPYFP HFFSPTN YGPS  SISAINSMKEMVKELHANGVEV++EVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           T+VDGALQGIDDSSYYF NRV NLE +SALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NAS LL+GHHGELLSRPP VEAIAFDP+LSKTK++ADFWDPQE +  ETRFPHWKRWAEV
Sbjct: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           +S LASELSWNCGEEGPT+  +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           VAFNDRRSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+QSRNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWED SCKFLAV+LRADKEEN SITE+ K RSDIFMAFNASDQSESVALPEPSEGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALPEPSEGTSWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSN 885
           R++DTAL FPGFFSSDGE VP TGS TYEMQAHSCALFEAKS+N
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEMQAHSCALFEAKSAN 884

BLAST of Tan0019462 vs. ExPASy TrEMBL
Match: A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1516.9 bits (3926), Expect = 0.0e+00
Identity = 739/885 (83.50%), Postives = 797/885 (90.06%), Query Frame = 0

Query: 1   MATSLSSLVIQPFCVCNCGITESFKLAVSDHHTYGHKIKYQSGKMDEARMVARGESKVGF 60
           MAT LSSLVIQPFCV NCG+TES KLA SDH TYG K KYQ GKMDEARM+A GE+KVG 
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  LKSSHRNLSKAYATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRS 120
           +KSSHRNLSK YA SGISV KSGQR GI GKSKE+ +VATYLFRTEFGDLVNVFV K  S
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 GFTVYVEMSSLQLTSVDGKPMLSWGVYRSDSAIVTPNFQSSLPDGTIGTTATPFVNTSEG 180
            FTV +E+ S+QL S+D   +LSWGVYRSDSA+VTPNF+SS PD T G T TPFV TSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 NFAVELEFDAKHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSI 240
            F+VELEFDAKH PFYLSF+LKYP+ V SG+SEIRSH+KTSF VPVGFGRGYPSPLGLSI
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 SGDGSINFSIFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVS 300
           SGDGS+NFSIFSS+AES+VLCLYNDS SEKP+LELDLDPYINRSGNIWHASFE A  FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFD 360
           YGY+CKGSK H NQD +EVSRI+VDPYAKIL  SIPK+ GQGLG  S+FLGQ+ KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLI 420
           WD EV PNLPMEKLFVYRLNV+ FT+DKSSQLP DIAGTFSGLTKKLLHF  LGVNAVL+
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLL 420

Query: 421 EPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTH 480
           EPIF FDEKEGPYFP HFFSPTN YGPS  SISAINSMKEMVKELHANGVEV+LEVV+TH
Sbjct: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480

Query: 481 TAVDGALQGIDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540
           T+ +GALQGIDDSSYYF NRV NLE KSALNCNYPIVQQLLLDSLRYWVTEFH+DGFCF+
Sbjct: 481 TSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFV 540

Query: 541 NASSLLRGHHGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEV 600
           NAS LLRGHHGELLSRPP VEAIAFDPLLSKTK++ADFWDPQE    ETRFPHWKRWAEV
Sbjct: 541 NASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEV 600

Query: 601 NSKFCNDIRDFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFS 660
           NSKFC+DIRDFFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARN GLPLVDLVSFS
Sbjct: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 660

Query: 661 SSKLASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGS 720
           +S LASELSWNCGEEGPTSN +VLEKRLKQIRNFIFVLFVSLGVPVL+MGDECGQSSGGS
Sbjct: 661 NSNLASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGS 720

Query: 721 VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSP 780
           VAFND+RSF+WDLLKT FGTQTTQFI FLSSFR++RFDL+Q+RNFLKGENIDW+ NNQSP
Sbjct: 721 VAFNDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSP 780

Query: 781 PQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEGTSWF 840
           PQWED SCKFLAV+LRADKEEN SITE+ K RS+IFM FNASDQSESVALPEP EGTSWF
Sbjct: 781 PQWEDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWF 840

Query: 841 RLIDTALSFPGFFSSDGEPVPATGSATYEMQAHSCALFEAKSSND 886
           R++DTAL FPGFFSSDGE VP TGS TYE+QAHSCALFEAKS+ND
Sbjct: 841 RVVDTALPFPGFFSSDGELVPMTGSVTYEIQAHSCALFEAKSAND 885

BLAST of Tan0019462 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 950.3 bits (2455), Expect = 1.1e-276
Identity = 479/877 (54.62%), Postives = 626/877 (71.38%), Query Frame = 0

Query: 14  CVCNCGITESFKLAVSDHHT-YGHKIKYQSGKMDEARMVARGESKVGFLKSSHRNLSKAY 73
           C  N GIT ++K   +   T   +KIK  S  +   R    G+     L+S     SK  
Sbjct: 13  CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQS--YQFSKIC 72

Query: 74  ATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRSGFTVYVEMSSLQ 133
           A S  S+E          ++ +  KV +Y FRT+ G LV V VEK R  +++ V +SSL+
Sbjct: 73  A-SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLE 132

Query: 134 LTSVD-GKPMLSWGVYRSDSAIVTP-NFQSSLPDGTIGTTATPFVNTSEGNFAVELEFDA 193
           L+  D  + ++ WGVYRSDS+   P +F++S  D    TT T FV +S     + LEFD 
Sbjct: 133 LSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDG 192

Query: 194 KHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSIS-GDGSINFS 253
           K  PFYLSF LK          E+ +HR T FC+PVGF  G+P PLGLS    D S NFS
Sbjct: 193 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFS 252

Query: 254 IFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVSYGYRCKGSK 313
            FS ++ +VVLCLY+DS ++KP LELDLDPY+NR+G++WHAS ++  +FV YGYRCK + 
Sbjct: 253 FFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETA 312

Query: 314 FHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFDWDREVRPNL 373
                 +VE   I++DPYA ++  S+          + ++LG + K P+FDW  +V PN+
Sbjct: 313 HSKEDVDVEGEPIVLDPYATVVGKSV----------SQKYLGSLSKSPSFDWGEDVSPNI 372

Query: 374 PMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLIEPIFPFDEK 433
           P+EKL VYRLNVK FT  +SS+LP+++AGTFSG+ +K+ H  TLG NAVL+EPIF F E+
Sbjct: 373 PLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQ 432

Query: 434 EGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTHTAVDGALQG 493
           +GPYFP HFFSP +IYGPS    SA+NSMK MVK+LH+ G+EV+LEVVFTHTA  GAL+G
Sbjct: 433 KGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRG 492

Query: 494 IDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGH 553
           IDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFH+DGFCFINASSLLRG 
Sbjct: 493 IDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGV 552

Query: 554 HGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIR 613
           HGE LSRPPLVEAIAFDPLL++TK+IAD WDP E +  E RFPHWKRWAE+N+++C ++R
Sbjct: 553 HGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVR 612

Query: 614 DFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELS 673
           +F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN+GL LVD+VSFS  +LASELS
Sbjct: 613 NFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELS 672

Query: 674 WNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGSVAFNDRRSF 733
           WNCGEEG T+ + VL++RLKQIRNF+F+ ++SLGVPVL+MGDECG S+ GS     R+ F
Sbjct: 673 WNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPF 732

Query: 734 DWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSPPQWEDPSCK 793
           DW+LL + FGTQ TQFI+F++S RA+R D++Q R+FLK ENI WY N+Q+ P+WEDP+ K
Sbjct: 733 DWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASK 792

Query: 794 FLAVILR--ADKEENGSITEDDKPRS-DIFMAFNASDQSESVALPEPSEGTSWFRLIDTA 853
           FLA+ ++  +++EE  S+ E ++P+S D+F+ FNASD  ESV LP   +G+ W RL+DTA
Sbjct: 793 FLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTA 852

Query: 854 LSFPGFFSSDGEPV----PATGSATYEMQAHSCALFE 880
           L FPGFFS +GE V    P      YEM+ +SC LFE
Sbjct: 853 LPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Tan0019462 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 950.3 bits (2455), Expect = 1.1e-276
Identity = 479/877 (54.62%), Postives = 626/877 (71.38%), Query Frame = 0

Query: 14  CVCNCGITESFKLAVSDHHT-YGHKIKYQSGKMDEARMVARGESKVGFLKSSHRNLSKAY 73
           C  N GIT ++K   +   T   +KIK  S  +   R    G+     L+S     SK  
Sbjct: 13  CCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDLVTSALQS--YQFSKIC 72

Query: 74  ATSGISVEKSGQRFGISGKSKERAKVATYLFRTEFGDLVNVFVEKTRSGFTVYVEMSSLQ 133
           A S  S+E          ++ +  KV +Y FRT+ G LV V VEK R  +++ V +SSL+
Sbjct: 73  A-SKTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKKREKYSILVYVSSLE 132

Query: 134 LTSVD-GKPMLSWGVYRSDSAIVTP-NFQSSLPDGTIGTTATPFVNTSEGNFAVELEFDA 193
           L+  D  + ++ WGVYRSDS+   P +F++S  D    TT T FV +S     + LEFD 
Sbjct: 133 LSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDG 192

Query: 194 KHIPFYLSFLLKYPVAVGSGNSEIRSHRKTSFCVPVGFGRGYPSPLGLSIS-GDGSINFS 253
           K  PFYLSF LK          E+ +HR T FC+PVGF  G+P PLGLS    D S NFS
Sbjct: 193 KESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFS 252

Query: 254 IFSSTAESVVLCLYNDSKSEKPVLELDLDPYINRSGNIWHASFESALNFVSYGYRCKGSK 313
            FS ++ +VVLCLY+DS ++KP LELDLDPY+NR+G++WHAS ++  +FV YGYRCK + 
Sbjct: 253 FFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETA 312

Query: 314 FHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSRFLGQVCKVPAFDWDREVRPNL 373
                 +VE   I++DPYA ++  S+          + ++LG + K P+FDW  +V PN+
Sbjct: 313 HSKEDVDVEGEPIVLDPYATVVGKSV----------SQKYLGSLSKSPSFDWGEDVSPNI 372

Query: 374 PMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLLHFITLGVNAVLIEPIFPFDEK 433
           P+EKL VYRLNVK FT  +SS+LP+++AGTFSG+ +K+ H  TLG NAVL+EPIF F E+
Sbjct: 373 PLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQ 432

Query: 434 EGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLEVVFTHTAVDGALQG 493
           +GPYFP HFFSP +IYGPS    SA+NSMK MVK+LH+ G+EV+LEVVFTHTA  GAL+G
Sbjct: 433 KGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSGALRG 492

Query: 494 IDDSSYYFVNRVVNLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGH 553
           IDDSSYY+  R  +L+ KS LNCNYP+VQQL+L+SLRYWVTEFH+DGFCFINASSLLRG 
Sbjct: 493 IDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGV 552

Query: 554 HGELLSRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIR 613
           HGE LSRPPLVEAIAFDPLL++TK+IAD WDP E +  E RFPHWKRWAE+N+++C ++R
Sbjct: 553 HGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVR 612

Query: 614 DFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVDLVSFSSSKLASELS 673
           +F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I+RN+GL LVD+VSFS  +LASELS
Sbjct: 613 NFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYISRNSGLSLVDIVSFSGPELASELS 672

Query: 674 WNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECGQSSGGSVAFNDRRSF 733
           WNCGEEG T+ + VL++RLKQIRNF+F+ ++SLGVPVL+MGDECG S+ GS     R+ F
Sbjct: 673 WNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESRKPF 732

Query: 734 DWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGNNQSPPQWEDPSCK 793
           DW+LL + FGTQ TQFI+F++S RA+R D++Q R+FLK ENI WY N+Q+ P+WEDP+ K
Sbjct: 733 DWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASK 792

Query: 794 FLAVILR--ADKEENGSITEDDKPRS-DIFMAFNASDQSESVALPEPSEGTSWFRLIDTA 853
           FLA+ ++  +++EE  S+ E ++P+S D+F+ FNASD  ESV LP   +G+ W RL+DTA
Sbjct: 793 FLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTA 852

Query: 854 LSFPGFFSSDGEPV----PATGSATYEMQAHSCALFE 880
           L FPGFFS +GE V    P      YEM+ +SC LFE
Sbjct: 853 LPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Tan0019462 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 383.3 bits (983), Expect = 5.4e-106
Identity = 251/705 (35.60%), Postives = 360/705 (51.06%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCLY-----NDSKSEKPVLELDLDPYINRSG 290
           G  SPLG+S   D  INF++FS  A SV LCL       D   +  ++EL LDP +N++G
Sbjct: 88  GEVSPLGVS-QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTG 147

Query: 291 NIWHASFES-ALNFVSYGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLG 350
           + WH   E   LN V YGYR  G          + S +L+DPYAK++      + G    
Sbjct: 148 DTWHICVEDLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGH--SSFGDSSQ 207

Query: 351 STSRFLGQV-CKVPAFDWDREVR-PNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSG 410
             ++F G    +   FDW  + + PN+P + L +Y +NV+ FT D+SS +   I G++ G
Sbjct: 208 KFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLG 267

Query: 411 LTKKLLHFITLGVNAVLIEPIFPFDEKE--------------GPYFPSHFFSPTNIYGPS 470
             +K+ H   LG+NAV + P+F FDE E                Y   +FF+P + Y   
Sbjct: 268 FIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG 327

Query: 471 LGS-ISAINSMKEMVKELHANGVEVMLEVVFTHT-------AVDGALQGIDDSSYYFV-- 530
            G  I A    KEMVK LH+ G+EV+L+VV+ HT           + +GID+  YY +  
Sbjct: 328 EGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP 387

Query: 531 -NRVVNLEG-KSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGHHGELLSR 590
            N+++N  G  + LNCN+P+V +L+LDSLR+WVTE+H+DGF F  AS L R   G  LS 
Sbjct: 388 NNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSA 447

Query: 591 PPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKRWAEVNSKFCNDIRDFFRGE- 650
           PPL+ AIA D +LS+ KIIA+ WD     L   +FP+W RWAE N  + +D+R F +G+ 
Sbjct: 448 PPLIRAIAKDSVLSRCKIIAEPWDCGGLYL-VGKFPNWDRWAEWNGMYRDDVRRFIKGDS 507

Query: 651 GLVSSLATRLCGSGDVFS-DGRGPAFSFNFIARNAGLPLVDLVSFS-----------SSK 710
           G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++           +  
Sbjct: 508 GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDG 567

Query: 711 LASELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGVPVLHMGDECG-------QS 770
                SWNCG EG T +A +   R +Q++NF   L +S G P++ MGDE G        S
Sbjct: 568 CNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNS 627

Query: 771 SGGSVAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLYQSRNFLKGENIDWYGN 830
            G   + N+ +  + D  K       ++ I F  S    + +     NFL    I W+ +
Sbjct: 628 YGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHE-----NFLTQGEITWHED 687

Query: 831 NQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFNASDQSESVALPEPSEG 882
           N     W++   KFLA  L            D     DI++AFNA D      +P+P  G
Sbjct: 688 N-----WDNSESKFLAFTL-----------HDGIGGRDIYVAFNAHDYFVKALIPQPPPG 747

BLAST of Tan0019462 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 336.7 bits (862), Expect = 5.8e-92
Identity = 229/690 (33.19%), Postives = 338/690 (48.99%), Query Frame = 0

Query: 231 GYPSPLGLSISGDGSINFSIFSSTAESVVLCL--YNDSKSEKPVLELDLDPYINRSGNIW 290
           G PSP G ++  DG +NFS++S+ + S  +CL   +D +  K   E+ LDP  NR+G++W
Sbjct: 72  GLPSPFGPTVRDDG-VNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVW 131

Query: 291 HASFESALNFVSYGYRCKGSKFHANQDEVEVSRILVDPYAKILTASIPKTCGQGLGSTSR 350
           H         + YGYR  G          + S IL+DPYAK   A I +     LG    
Sbjct: 132 HVFLRGDFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAK---AIISRDEFGVLGPDDN 191

Query: 351 FLGQV-CKVPA----FDWDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGL 410
              Q+ C VP     FDW+ ++   LP + L +Y ++V+ FT  +SS++  +  GT+ G+
Sbjct: 192 CWPQMACMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGV 251

Query: 411 TKKLLHFITLGVNAVLIEPIFPFDEKE---------------GPYFPSHFFSPTNIYGPS 470
            +KL H   LG+N + + P   F+E E                 Y    FFSP   Y  +
Sbjct: 252 AEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASA 311

Query: 471 LGSI---SAINSMKEMVKELHANGVEVMLEVVFTHTAVDG------ALQGIDDSSYYFV- 530
             +     AIN  K +VKE H  G+EV+++VV  HTA         + +G+D+S YY + 
Sbjct: 312 SSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLA 371

Query: 531 --NRVVNLEG-KSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFINASSLLRGH------ 590
                 N  G  +  NCN+P+V+Q +LD LRYWVTE H+DGF F   S + R        
Sbjct: 372 PKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAA 431

Query: 591 -------HGELL------SRPPLVEAIAFDPLLSKTKIIADFWDPQESILNETRFPHWKR 650
                   G+LL      S PP+++ I+ DP+L   K+IA+ WD    +     FPHW  
Sbjct: 432 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWD-AGGLYQVGMFPHWGI 491

Query: 651 WAEVNSKFCNDIRDFFRG-EGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNAGLPLVD 710
           W+E N KF + +R F +G +G   + A  LCGS +++  GR P  S NFI  + G  L D
Sbjct: 492 WSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLAD 551

Query: 711 LVSFSS-SKLAS----------ELSWNCGEEGPTSNAEVLEKRLKQIRNFIFVLFVSLGV 770
           LV++++ + LA+            SWNCGEEG  ++  V   R +Q+RNF   L VS GV
Sbjct: 552 LVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGV 611

Query: 771 PVLHMGDECGQSSGGS----VAFNDRRSFDWDLLKTGFGTQTTQFITFLSSFRAKRFDLY 830
           P+++MGDE G + GG+       N    F WD  K    +   +F   L  FR +   L 
Sbjct: 612 PMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRDECESL- 671

Query: 831 QSRNFLKGENIDWYGNNQSPPQWEDPSCKFLAVILRADKEENGSITEDDKPRSDIFMAFN 851
              +F   + + W+G     P W + S +F+A  L             D  + +I++AFN
Sbjct: 672 GLNDFPTAKRLQWHGLAPEIPNWSETS-RFVAFSL------------VDSVKKEIYVAFN 731

BLAST of Tan0019462 vs. TAIR 10
Match: AT3G20440.2 (Alpha amylase family protein )

HSP 1 Score: 58.2 bits (139), Expect = 4.0e-08
Identity = 54/202 (26.73%), Postives = 91/202 (45.05%), Query Frame = 0

Query: 358 AFDWDREVRPNLPMEKLFVYRLNVKHFTVDKSSQLPTDIAGTFSGLTKKLL-HFITLGVN 417
           A+ W +  +P +P E L +Y  +     V  S   P     TF   TKK+L H    G N
Sbjct: 384 AYKW-KYSKPKVP-ESLRIYECH-----VGISGSEPK--VSTFEEFTKKVLPHVKRAGYN 443

Query: 418 AV-LIEPIFPFDEKEGPYFPSHFFSPTNIYGPSLGSISAINSMKEMVKELHANGVEVMLE 477
           A+ LI      D     Y  ++FF+ ++ YG         +  K +V E H  G+ V L+
Sbjct: 444 AIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTP-------DDFKRLVDEAHGLGLLVFLD 503

Query: 478 VVFTHTAVD-----GALQGIDDSSYYFVNRVVNLE-GKSALNCNYPIVQQLLLDSLRYWV 537
           +V ++ A D         G +D  +++  R  +   G          V   L+ +L +W+
Sbjct: 504 IVHSYAAADQMVGLSLFDGSNDCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWI 563

Query: 538 TEFHIDGFCFINASSLLRGHHG 552
           TE+ +DG+ F + +S++  H+G
Sbjct: 564 TEYQVDGYQFHSLASMIYTHNG 569