Tan0018751 (gene) Snake gourd v1

BLAST of Tan0018751 vs. ExPASy Swiss-Prot
Match: Q9LZA5 (Nuclear intron maturase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT3 PE=3 SV=2)

HSP 1 Score: 840.5 bits (2170), Expect = 1.5e-242
Identity = 445/739 (60.22%), Postives = 547/739 (74.02%), Query Frame = 0

Query: 39  FQHSAETLTRP----QLKALVLSRFSHGKFFDLLQNVVASPSVLLTASQNLITPPPTNRL 98
           F +S +T+T P    +L+ALVL ++SHGKF+ L++N V+ P VLL A QNL        L
Sbjct: 33  FLNSDQTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNL-------SL 92

Query: 99  NALDSLPILDRVSNCFSVQDMARELYQNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLI 158
           +A  S  + DRVS  FS+++M RE+ + RFD+ +CCV    S     SLVLPNLKLKVLI
Sbjct: 93  SANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISS-----SLVLPNLKLKVLI 152

Query: 159 EAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHV 218
           EAIRMVLEIVYD+RF TFSYGGRVGMGRHTAIRYLKNSV+NP WWF V+F R+ F+  +V
Sbjct: 153 EAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNV 212

Query: 219 NKLCLLVQEKIEDDILIFMLKKLFELGAIQIELGACYLGRGFPQESGLCSILFNIYFNGF 278
           + LC  V EKI D +LI M+KKLFE G ++IELG C  GRGFPQE GLCSIL N+YF+G 
Sbjct: 213 DILCGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGL 272

Query: 279 DKEIQQIRLQKNEENPKFSPDDNVS----FHNPVKIYAVRYFDEILVITSGSKMLTMELK 338
           DKEIQ +RL+   +NP+    D  S    F  PV IYAVRY DEILVITSGSKMLTM+LK
Sbjct: 273 DKEIQDLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDLK 332

Query: 339 SQVLKYLEGNLELEVDRLNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRAQKKY 398
            +++  LE  LEL VDRLNT+IHSAVSEKI+FLGM LQAVPPSVL PP SEKA+RA KKY
Sbjct: 333 KRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKKY 392

Query: 399 LRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTDGFKSEFQIEKEVREIFRNWSDE 458
            RQK+VR +ELRNARERNRK LGLKI  HV KK+KQ++GFK E +IE EVR+IF++W +E
Sbjct: 393 QRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGEE 452

Query: 459 VVQDFFESLEDHAEWYRALTTGYFLSLKHIRNQLPEDLVNAYDRFQEQVDKHLNPVKIKK 518
           V+QDF  SLE+  +W+  LT G FLSL+HIR +LP+DL++AYD FQEQVDKHL P + K 
Sbjct: 453 VMQDFMGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK- 512

Query: 519 EKAREDEEKRAEEEEQ--YAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPQPI 578
            K  EDEE+R EEEE+  YA+RTVEDLT+LC+KV AP ELVRKA+K+VGFTN MGRP+PI
Sbjct: 513 -KVLEDEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAIKLVGFTNSMGRPRPI 572

Query: 579 CSLVALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTVVTYHLRFSCILTLAEKHESTKR 638
             LV LED+DIIKWYA                                    EKH STK+
Sbjct: 573 IHLVTLEDSDIIKWYA----------------------------------RHEKHGSTKK 632

Query: 639 EAMKHYSKDLKVFDLNGNEEMHFPTEREVKMLGDRNLSDPYPVDGALSLFLIRLATDEAS 698
             ++HY+KDL+V DL+G EE HFP+EREVKM+GD+NLSDP PVDG LSL LIRLA+DE  
Sbjct: 633 -LIRHYTKDLRVSDLDGREEAHFPSEREVKMMGDKNLSDPKPVDGTLSLLLIRLASDEPL 692

Query: 699 YPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGVEWVRGMGVIHESLNQRCLPLCADHISD 758
           + C A FC R+D+I++RV LLQ  L++NP +  +WV GMG IH +LN++CLPLC+ HISD
Sbjct: 693 HHCAASFCERSDTIMHRVHLLQNRLHINPLDEEKWVPGMGTIHSALNRKCLPLCSTHISD 722

Query: 759 LYMGKINLQDLDCTSSLDM 768
           +Y+GKI LQD+D +S +D+
Sbjct: 753 VYLGKITLQDVDSSSFIDL 722

BLAST of Tan0018751 vs. ExPASy Swiss-Prot
Match: Q9CA78 (Nuclear intron maturase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NMAT4 PE=3 SV=2)

HSP 1 Score: 217.2 bits (552), Expect = 6.3e-55
Identity = 189/756 (25.00%), Postives = 329/756 (43.52%), Query Frame = 0

Query: 50  QLKALVLSRFSHGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNC 109
           +LK  V  +  +GKF DLL+ V+A P  L  A   +       RLN+  ++ I +R +  
Sbjct: 90  RLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCI-------RLNS--NVSITER-NGS 149

Query: 110 FSVQDMARELYQNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERF 169
            +   +A EL    FD+ +    +    +  + LVLP++ LKV+ EAIR+VLE+V+   F
Sbjct: 150 VAFDSIAEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIRIVLEVVFSPHF 209

Query: 170 VTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDI 229
              S+  R G GR +A++Y+ N++    W F ++ ++K   SV  N L ++ +EK+ED  
Sbjct: 210 SKISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLSVM-EEKVEDSS 269

Query: 230 LIFMLKKLFELGAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQI-------- 289
           L  +L+ +FE   + +E G    G G PQE  L  +L NIY + FD E  +I        
Sbjct: 270 LSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEFYRISMRHEALG 329

Query: 290 ------------------RLQKNEENPKFSPDDNVSFHNPVKIYAVRYFDEILVITSGSK 349
                             R Q  E+  K + + +V+    +++Y  R+ DEI    SG K
Sbjct: 330 LDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVA----LRVYCCRFMDEIYFSVSGPK 389

Query: 350 MLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKA 409
            +  +++S+ + +L  +L L++           +  +  LG     V  +V   P     
Sbjct: 390 KVASDIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG---TLVRKNVRESP----T 449

Query: 410 IRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTD--------------- 469
           ++A  K   +  + A++   A      ++G K L H  KK+K+++               
Sbjct: 450 VKAVHKLKEKVRLFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKGLADSNSTLSQIS 509

Query: 470 -GFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHIRNQLPED 529
              K+  + +   + + R W ++V++   +  E+           + LS   +   +P++
Sbjct: 510 CHRKAGMETDHWYKILLRIWMEDVLRTSADRSEE-----------FVLSKHVVEPTVPQE 569

Query: 530 LVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCIKVDAPIE 589
           L +A+ +FQ     +++              + A  E         D       V AP  
Sbjct: 570 LRDAFYKFQNAAAAYVS-------------SETANLEALLPCPQSHDRPVFFGDVVAPTN 629

Query: 590 LVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTVV 649
            + + +   G     G  +    L+ L+   II WY+G+ RRW+ ++  C N+  +K ++
Sbjct: 630 AIGRRLYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDEIKALI 689

Query: 650 TYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKMLGDRNLSD 709
              +R SCI TLA K+   + E  K    +L       + E     E+      DR+   
Sbjct: 690 DNQIRMSCIRTLAAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSPAFDRDEHL 749

Query: 710 PYPV--DGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGVEWVR 761
            Y +   G   L L RL ++     C    C+     +Y +  ++R           W  
Sbjct: 750 TYGLSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------QKFPGWKT 793

BLAST of Tan0018751 vs. ExPASy Swiss-Prot
Match: P03876 (Putative COX1/OXI3 intron 2 protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AI2 PE=4 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 5.1e-12
Identity = 64/262 (24.43%), Postives = 118/262 (45.04%), Query Frame = 0

Query: 143 LVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFAV 202
           L + N + K++ E++RM+LEI+Y+  F  +S+G R  +   TAI   KN +Q  +W+  V
Sbjct: 358 LSVGNPREKIVQESMRMMLEIIYNNSFSYYSHGFRPNLSCLTAIIQCKNYMQYCNWFIKV 417

Query: 203 AFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFELGAIQIELGACYLGRGFPQESGL 262
             + K FD++  N L  ++ E+I+D   + +L KL   G +           G PQ S +
Sbjct: 418 DLN-KCFDTIPHNMLINVLNERIKDKGFMDLLYKLLRAGYVDKNNNYHNTTLGIPQGSVV 477

Query: 263 CSILFNIYF--------NGFDKEIQQIRLQKNEENPKFSPDDNVSFHNPV---------- 322
             IL NI+         N F+ E     +     NP ++   +  +   +          
Sbjct: 478 SPILCNIFLDKLDKYLENKFENEFNTGNMSNRGRNPIYNSLSSKIYRCKLLSEKLKLIRL 537

Query: 323 ---------------KIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLN 372
                          + Y VRY D+I++   GS      + + +  +L+ NL + ++ ++
Sbjct: 538 RDHYQRNMGSDKSFKRAYFVRYADDIIIGVMGSHNDCKNILNDINNFLKENLGMSIN-MD 597

BLAST of Tan0018751 vs. ExPASy Swiss-Prot
Match: P05511 (Uncharacterized 91 kDa protein in cob intron OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPMIT.06 PE=4 SV=4)

HSP 1 Score: 74.7 bits (182), Expect = 5.1e-12
Identity = 76/304 (25.00%), Postives = 138/304 (45.39%), Query Frame = 0

Query: 99  SLPILDRVSNCFSVQDMARELYQNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIR 158
           S+ ++D++     +Q +  E +   F  G   +    SG K + L + + + K++ E +R
Sbjct: 274 SIDVIDKI-----IQSLKSEEF--NFTPGRRILIDKASGGK-RPLTIGSPRDKLVQEILR 333

Query: 159 MVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLC 218
           +VLE +Y+  F T S+G R G   H+A+R +  + +  +WW      +  FDS+  +KL 
Sbjct: 334 IVLEAIYEPLFNTASHGFRPGRSCHSALRSIFTNFKGCTWWIEGDI-KACFDSIPHDKLI 393

Query: 219 LLVQEKIEDDILIFMLKKLFELGAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEI 278
            L+  KI+D   I +++K    G +  E    Y   G PQ S +  IL NIY +  D+ I
Sbjct: 394 ALLSSKIKDQRFIQLIRKALNAGYL-TENRYKYDIVGTPQGSIVSPILANIYLHQLDEFI 453

Query: 279 QQIRLQKNEENPKFSPDDNVSFH-----------------------------NPV----- 338
           + ++ + + + P      + S H                             N +     
Sbjct: 454 ENLKSEFDYKGPIARKRTSESRHLHYLMAKAKRENADSKTIRKIAIEMRNVPNKIHGIQS 513

Query: 339 -KIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKISFL 368
            K+  VRY D+ +V  +GS   T E+ +++  +   ++ L V    T I ++ ++KI FL
Sbjct: 514 NKLMYVRYADDWIVAVNGSYTQTKEILAKITCFC-SSIGLTVSPTKTKITNSYTDKILFL 566

BLAST of Tan0018751 vs. ExPASy Swiss-Prot
Match: B1N1A3 (Putative nicotine oxidoreductase OS=Pseudomonas putida OX=303 GN=nicA PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 4.3e-11
Identity = 70/264 (26.52%), Postives = 119/264 (45.08%), Query Frame = 0

Query: 151 KVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFAVAFHRKKFD 210
           KV+ E IR +LE +Y+  F   S+G R G   HTA++ ++ S    +W       +  FD
Sbjct: 109 KVVQEVIRSILEAIYEPTFSKNSHGFRAGKSCHTALKQVRESWSGVTWVIEGDI-KGCFD 168

Query: 211 SVHVNKLCLLVQEKIEDDILIFMLKKLFELGAIQIELGACYLGR-GFPQESGLCSILFNI 270
           ++  +KL   ++ +I+D+  I +++K    G    E GA +    G PQ S +  IL N+
Sbjct: 169 NISHSKLIDQLRLRIKDERFINLIRKALNAG--YFENGAFFSATLGTPQGSIISPILANV 228

Query: 271 YFNGFDKEIQQI-------------------RLQ--------KNEENPKFSPDDNVSF-- 330
           + +  D++++Q+                   +LQ        K E+      D  +S   
Sbjct: 229 FLDQLDRKVEQLIKDHHQGEEGDKITDPAYRKLQRQKTSLRKKAEKQEGAERDATLSLAR 288

Query: 331 ----------------HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELE 369
                           +  +++  VRY D+ ++  +G K+L  EL+S V ++LE N  LE
Sbjct: 289 EANSKLLSMSPYLTRNNGFIRVKYVRYADDWIIGVNGPKLLAEELRSVVGEFLE-NAGLE 348

BLAST of Tan0018751 vs. NCBI nr
Match: KAG6600779.1 (Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1380.5 bits (3572), Expect = 0.0e+00
Identity = 690/768 (89.84%), Postives = 729/768 (94.92%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHSPLLELPFQHSAETLTRPQLKALVLSRFS 60
           MLIHLRKIATPLKPKLS+SFNKLYSD PLKHSPLL+LPFQHS +TLTRPQL+ALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  HGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNCFSVQDMARELY 120
            GKFFDLLQNVVASPSVL TASQNLITP P+NRLNA +SL  LD VS+CFSV+DMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120

Query: 121 QNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180
           +NRFD+GACCVR+ PS EKG+ LVLPNLKLKVL+EAIRMVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWF VAF R+KFDSVHVNKLCLLVQEKI+DDILI MLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 GAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNVSF 300
            A+QIELG CYLGRGFPQESGLCSIL NIYFNGFDKEIQQIRLQKNEENPKFS D  VSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSMDGTVSF 300

Query: 301 HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKI 360
           HNPVKIYAVRY DEILVITSGSKML MELKSQVL+YLEGNLELEVDR+NTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRA+KKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHI 480
           FKKLK+TDG KSEFQIEKEV EIFRNW+DEVV+DFFESLED+ EW+R L+ G FLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCI 540
           RNQLP DLVNAYDRFQ+QV++HLNP+K K+EKAREDEEKR  EEE+YAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KV+APIELVRKAVKM+GFTNKMGRP+PI SL+ALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKML 660
           KMVKT+VTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNG EEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 720
           G+RNL+DPYPVDGA SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCT SLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Tan0018751 vs. NCBI nr
Match: KAG7031416.1 (Nuclear intron maturase 3, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1379.0 bits (3568), Expect = 0.0e+00
Identity = 689/768 (89.71%), Postives = 729/768 (94.92%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHSPLLELPFQHSAETLTRPQLKALVLSRFS 60
           MLIHLRKIATPLKPKLS+SFNKLYSD PLKHSPLL+LPFQHS +TLTRPQL+ALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSFNKLYSDSPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  HGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNCFSVQDMARELY 120
            GKFFDLLQNVVASPSVL TASQNLITP P+NRLNA +SL  LD VS+CFSV+DMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120

Query: 121 QNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180
           +NRFD+GACCVR+ PS EKG+ LVLPNLKLKVL+EAIRMVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLEPSEEKGEFLVLPNLKLKVLLEAIRMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWF VAF R+KFDSVHVNKLCLLVQEKI+DDILI MLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 GAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNVSF 300
            A+QIELG CYLGRGFPQESGLCSIL NIYFNGFDKEIQQIRLQKNEENPKFS D  VSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLDGTVSF 300

Query: 301 HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKI 360
           HNPVKIYAVRY DEILVITSGSKML MELKSQVL+YLEGNLELEVDR+NTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRA+KKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHI 480
           FKKLK+TDG KSEFQIEKEV EIFRNW+DEVV+DFFESLED+ EW+R L+ G FLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCI 540
           RNQLP DLVNAYDRFQ+QV++HLNP+K K+EKAREDEEKR  EEE+YAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KV+APIELVRKAVKM+GFTNKMGRP+PI SL+ALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKML 660
           KMVKT+VTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNG EEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 720
           G+RNL+DPYPVDGA SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHI+DLYMGKINLQDLDCT SLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHINDLYMGKINLQDLDCTLSLDMD 768

BLAST of Tan0018751 vs. NCBI nr
Match: XP_022976163.1 (nuclear intron maturase 3, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 687/768 (89.45%), Postives = 726/768 (94.53%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHSPLLELPFQHSAETLTRPQLKALVLSRFS 60
           MLIHLRKIATPLKPKLS+S NKLYSD PLKHSPLL+LPFQHS +TLTRPQL+ALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSLNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  HGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNCFSVQDMARELY 120
            GKFFDLLQNVVASPSVL TASQNLITP P+NRLNA +SL  LD VS+CFSV+DMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120

Query: 121 QNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180
           +NRFD+GACCVR+  S EKG+ LVLPNLKLKVL+EA++MVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAMKMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWF VAF R+KFDSVHVNKLCLLVQEKI+DDILI MLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 GAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNVSF 300
            A+QIELG CYLGRGFPQESGLCSIL NIYFNGFDKEIQQIRLQKNEENPKFS +  VSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLNGTVSF 300

Query: 301 HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKI 360
           HNPVKIYAVRY DEILVITSGSKM  MELKSQVL+YLEGNLELEVDR+NTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMQIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRA+KKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHI 480
           FKKLK+TDG KSEFQIEKEV EIFRNW+DEVV+DFFESLED+ EW+R L+ G FLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCI 540
           RNQLP DLVNAYDRFQ+QV+KHLNP+K KKEKAREDEEKR  EEE+YAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNKHLNPLKAKKEKAREDEEKRLVEEERYAKRTVEDLTRLCI 540

Query: 541 KVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KV+APIELVRKAVKM+GFTNKMGRPQPI SL+ALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPQPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKML 660
           KMVKT+VTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNG EEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 720
           G+RNL+DPYPVDGA SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCT SLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Tan0018751 vs. NCBI nr
Match: XP_022942578.1 (nuclear intron maturase 3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1369.8 bits (3544), Expect = 0.0e+00
Identity = 686/768 (89.32%), Postives = 726/768 (94.53%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHSPLLELPFQHSAETLTRPQLKALVLSRFS 60
           MLIHLRKIATPLKPKLS+SFNKLYSD PLKHSPLL+LPFQHS +TLTRPQL+ALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSFNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  HGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNCFSVQDMARELY 120
            GKFFDLLQNVVASPSVL TASQNLITP P+NRLNA DSL  LD VS+CFSV+DMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSLDMVSSCFSVEDMARELY 120

Query: 121 QNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180
           +NRFD+GACCVR+  S EKG+ LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWF VAF R+KFDSVHVNKLCLLVQEKI+DDILI MLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 GAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNVSF 300
            A+QIELG CYLGRG PQESGLCSIL NIYFNGFDKEIQQIRL+KNEENPKFS D  VSF
Sbjct: 241 EAVQIELGGCYLGRGLPQESGLCSILSNIYFNGFDKEIQQIRLEKNEENPKFSLDGTVSF 300

Query: 301 HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKI 360
           HNPVKIYAVRY DEILVITSGSKML MELKSQVL+YLEGNLELEVDR+NTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAV PSVLHPPMSEKAIRA+KKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVLPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHI 480
           FKKLK+TDG KSEFQIEKEV EIFRNW+DEVV+DFFESLED+ EW+R L+ G FLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCI 540
           RNQLP DLVNAYDRFQ+QV++HLNP+K K+EKAREDEEKR  EEE+YAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KV+APIELVRKAVKM+GFTNKMGRP+PI SL+ALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKML 660
           KMVKT+VTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNG EEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 720
           G+RNL+DPYPVDGA SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCT SLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Tan0018751 vs. NCBI nr
Match: XP_023544815.1 (nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544823.1 nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544830.1 nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023544839.1 nuclear intron maturase 3, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1359.4 bits (3517), Expect = 0.0e+00
Identity = 679/768 (88.41%), Postives = 721/768 (93.88%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHSPLLELPFQHSAETLTRPQLKALVLSRFS 60
           MLIHLRKIATP KP LS+SFNKLYSD PLKHSPLL+LPFQHS +TLTRPQL+ALVLSRFS
Sbjct: 1   MLIHLRKIATPWKPNLSSSFNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  HGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNCFSVQDMARELY 120
            GKFFDLL+NVVASPSVL TASQNLITP P+NRLNA DSL   D VS+CFSV+DMARELY
Sbjct: 61  QGKFFDLLRNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSFDMVSSCFSVEDMARELY 120

Query: 121 QNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180
           +NRFD+GACCVR+  S EKG+ LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWF VAF R+KFDSVHVNKLCLLVQEKI+DDILI MLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 GAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNVSF 300
            A+QIELG CYLGRGFPQESGLCSIL NIYFNGFDKE+QQIRL+KNEENPKFS D  VSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKELQQIRLEKNEENPKFSLDGTVSF 300

Query: 301 HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKI 360
           HNPVKIYAVRY DEILVITSGSKML MELKSQVL+YLEGNLELEVDR+NTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRA+KKYLRQKEVRAIELRNARERNRKKLGLKIL HV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILGHV 420

Query: 421 FKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHI 480
           FKKLK+TDG K EFQIEKEV EIFRNW+DEVV+DF ESLED+ EW+R L+ G FLSLKHI
Sbjct: 421 FKKLKRTDGLKYEFQIEKEVTEIFRNWADEVVRDFLESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCI 540
           RNQLP DLVNAYDRFQ+QV++HLNP+K KKEKAREDE+KR  EEE+YAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKKEKAREDEKKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KV+APIELVRKAVKM+GFTNKMGRP+PI SL+ALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKML 660
           K VKT+VTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNG EEMHFPTEREVKML
Sbjct: 601 KTVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 720
           G+RNL+DPYPVDGA SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCT SLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Tan0018751 vs. ExPASy TrEMBL
Match: A0A6J1ILC2 (nuclear intron maturase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111476629 PE=4 SV=1)

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 687/768 (89.45%), Postives = 726/768 (94.53%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHSPLLELPFQHSAETLTRPQLKALVLSRFS 60
           MLIHLRKIATPLKPKLS+S NKLYSD PLKHSPLL+LPFQHS +TLTRPQL+ALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSLNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  HGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNCFSVQDMARELY 120
            GKFFDLLQNVVASPSVL TASQNLITP P+NRLNA +SL  LD VS+CFSV+DMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPESLLSLDMVSSCFSVEDMARELY 120

Query: 121 QNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180
           +NRFD+GACCVR+  S EKG+ LVLPNLKLKVL+EA++MVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAMKMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWF VAF R+KFDSVHVNKLCLLVQEKI+DDILI MLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 GAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNVSF 300
            A+QIELG CYLGRGFPQESGLCSIL NIYFNGFDKEIQQIRLQKNEENPKFS +  VSF
Sbjct: 241 EAVQIELGGCYLGRGFPQESGLCSILSNIYFNGFDKEIQQIRLQKNEENPKFSLNGTVSF 300

Query: 301 HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKI 360
           HNPVKIYAVRY DEILVITSGSKM  MELKSQVL+YLEGNLELEVDR+NTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMQIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAVPPSVLHPPMSEKAIRA+KKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVPPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHI 480
           FKKLK+TDG KSEFQIEKEV EIFRNW+DEVV+DFFESLED+ EW+R L+ G FLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCI 540
           RNQLP DLVNAYDRFQ+QV+KHLNP+K KKEKAREDEEKR  EEE+YAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNKHLNPLKAKKEKAREDEEKRLVEEERYAKRTVEDLTRLCI 540

Query: 541 KVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KV+APIELVRKAVKM+GFTNKMGRPQPI SL+ALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPQPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKML 660
           KMVKT+VTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNG EEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 720
           G+RNL+DPYPVDGA SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCT SLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Tan0018751 vs. ExPASy TrEMBL
Match: A0A6J1FV64 (nuclear intron maturase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111447574 PE=4 SV=1)

HSP 1 Score: 1369.8 bits (3544), Expect = 0.0e+00
Identity = 686/768 (89.32%), Postives = 726/768 (94.53%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHSPLLELPFQHSAETLTRPQLKALVLSRFS 60
           MLIHLRKIATPLKPKLS+SFNKLYSD PLKHSPLL+LPFQHS +TLTRPQL+ALVLSRFS
Sbjct: 1   MLIHLRKIATPLKPKLSSSFNKLYSDRPLKHSPLLKLPFQHSVQTLTRPQLEALVLSRFS 60

Query: 61  HGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNCFSVQDMARELY 120
            GKFFDLLQNVVASPSVL TASQNLITP P+NRLNA DSL  LD VS+CFSV+DMARELY
Sbjct: 61  QGKFFDLLQNVVASPSVLFTASQNLITPLPSNRLNAPDSLLSLDMVSSCFSVEDMARELY 120

Query: 121 QNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180
           +NRFD+GACCVR+  S EKG+ LVLPNLKLKVL+EAIR+VLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVRLESSEEKGEFLVLPNLKLKVLLEAIRIVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWF VAF R+KFDSVHVNKLCLLVQEKI+DDILI MLKKLFEL
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRRKFDSVHVNKLCLLVQEKIKDDILILMLKKLFEL 240

Query: 241 GAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNVSF 300
            A+QIELG CYLGRG PQESGLCSIL NIYFNGFDKEIQQIRL+KNEENPKFS D  VSF
Sbjct: 241 EAVQIELGGCYLGRGLPQESGLCSILSNIYFNGFDKEIQQIRLEKNEENPKFSLDGTVSF 300

Query: 301 HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKI 360
           HNPVKIYAVRY DEILVITSGSKML MELKSQVL+YLEGNLELEVDR+NTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLIMELKSQVLRYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
           SFLGMELQAV PSVLHPPMSEKAIRA+KKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 SFLGMELQAVLPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHI 480
           FKKLK+TDG KSEFQIEKEV EIFRNW+DEVV+DFFESLED+ EW+R L+ G FLSLKHI
Sbjct: 421 FKKLKRTDGLKSEFQIEKEVTEIFRNWADEVVRDFFESLEDNTEWHRPLSAGDFLSLKHI 480

Query: 481 RNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCI 540
           RNQLP DLVNAYDRFQ+QV++HLNP+K K+EKAREDEEKR  EEE+YAKRTVEDLTRLCI
Sbjct: 481 RNQLPVDLVNAYDRFQDQVNQHLNPLKAKEEKAREDEEKRLGEEERYAKRTVEDLTRLCI 540

Query: 541 KVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KV+APIELVRKAVKM+GFTNKMGRP+PI SL+ALEDTDIIKWYAGVGRRWLDFFCCCHNY
Sbjct: 541 KVEAPIELVRKAVKMIGFTNKMGRPRPISSLIALEDTDIIKWYAGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKML 660
           KMVKT+VTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNG EEMHFPTEREVKML
Sbjct: 601 KMVKTIVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGKEEMHFPTEREVKML 660

Query: 661 GDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 720
           G+RNL+DPYPVDGA SLFLIRL TDE SYPCIAHFCNRTDSILYRVRLLQ+TLNVNPSNG
Sbjct: 661 GERNLADPYPVDGAFSLFLIRLVTDEDSYPCIAHFCNRTDSILYRVRLLQKTLNVNPSNG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCT SLDMD
Sbjct: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 768

BLAST of Tan0018751 vs. ExPASy TrEMBL
Match: A0A6J1DNH6 (nuclear intron maturase 3, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111022853 PE=4 SV=1)

HSP 1 Score: 1337.4 bits (3460), Expect = 0.0e+00
Identity = 681/770 (88.44%), Postives = 718/770 (93.25%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHS-PLLELPFQHSAETLTRPQLKALVLSRF 60
           M I LRKIATPLK  LSTSFNKLYSDLPLKHS PL   PFQHS ETLT P+LKALVLSRF
Sbjct: 1   MFIPLRKIATPLKSILSTSFNKLYSDLPLKHSPPLHNFPFQHSTETLTWPELKALVLSRF 60

Query: 61  SHGKFFDLLQNVVASPSVLLTASQNLIT-PPPTNRLNALDSLPILDRVSNCFSVQDMARE 120
           +HGKF DLLQNVVASPSVLLTASQNLIT PPP+N LNA D LPILD VS CFSV++MARE
Sbjct: 61  THGKFLDLLQNVVASPSVLLTASQNLITPPPPSNGLNAPDPLPILDLVSKCFSVEEMARE 120

Query: 121 LYQNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRV 180
           LY++RFD+GACCVRM    +KGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRV
Sbjct: 121 LYEDRFDVGACCVRM---DQKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRV 180

Query: 181 GMGRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLF 240
           GMGRHTAIRYLKNSVQNPSWWF VAF RKKFDSVHV+KLCLLV+EKI+D +LI MLKKLF
Sbjct: 181 GMGRHTAIRYLKNSVQNPSWWFTVAFRRKKFDSVHVHKLCLLVEEKIKDHLLICMLKKLF 240

Query: 241 ELGAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNV 300
           EL AIQIELGACYLGRGFPQESGLCSIL NI+FNGFDK+IQQIRLQKNEENPKFS D+ V
Sbjct: 241 ELEAIQIELGACYLGRGFPQESGLCSILCNIFFNGFDKDIQQIRLQKNEENPKFSLDEIV 300

Query: 301 SFHNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSE 360
           SFH+PVKIYAVRY DEILVITSGSKMLTM+LKSQVLKYLEGNLELEVDR+NTAIHSAVSE
Sbjct: 301 SFHSPVKIYAVRYLDEILVITSGSKMLTMDLKSQVLKYLEGNLELEVDRMNTAIHSAVSE 360

Query: 361 KISFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILS 420
           KISFLGMELQAVPPSVLHPPMSEKAIRA+KKY+RQKEVR IELRNARERNRKKLGLKILS
Sbjct: 361 KISFLGMELQAVPPSVLHPPMSEKAIRARKKYIRQKEVRVIELRNARERNRKKLGLKILS 420

Query: 421 HVFKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLK 480
           HVFKKLKQTDGFK EFQIEKEVREIFRNW+DEV Q FFESLE+HAEW+ AL+ G FLSLK
Sbjct: 421 HVFKKLKQTDGFKFEFQIEKEVREIFRNWADEVAQHFFESLENHAEWHHALSAGDFLSLK 480

Query: 481 HIRNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRL 540
           HIRNQLPEDLVNAYDRFQ+QVDKHLNPVK+K  KAREDEEKR EEE++YA+RTVEDLTRL
Sbjct: 481 HIRNQLPEDLVNAYDRFQDQVDKHLNPVKVKYVKAREDEEKRVEEEQKYARRTVEDLTRL 540

Query: 541 CIKVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCH 600
           CIKVDAPIELVRKAVKMVGFTNKMGRPQPI  LVALED DIIKWYAGVGRRWLDFFCCCH
Sbjct: 541 CIKVDAPIELVRKAVKMVGFTNKMGRPQPISLLVALEDIDIIKWYAGVGRRWLDFFCCCH 600

Query: 601 NYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVK 660
           NYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKH+SKDLKVFDLNG+EE+HFPTEREVK
Sbjct: 601 NYKMVKTVVTYHLRFSCILTLAEKHESTKREAMKHFSKDLKVFDLNGDEEIHFPTEREVK 660

Query: 661 MLGDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPS 720
           MLGDR L+DPYPVDG LSLFLIRLA DE S PCIAHFCNRTDSILYRVRLLQRTLNVN  
Sbjct: 661 MLGDRMLADPYPVDGTLSLFLIRLAIDEPSCPCIAHFCNRTDSILYRVRLLQRTLNVNSC 720

Query: 721 NGVEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           +G +WVRGMGVIHESLNQRCLPLCADHISDLYMG+INLQDLDCT SLDMD
Sbjct: 721 DGEKWVRGMGVIHESLNQRCLPLCADHISDLYMGRINLQDLDCTLSLDMD 767

BLAST of Tan0018751 vs. ExPASy TrEMBL
Match: A0A1S4E4Y1 (uncharacterized protein LOC103501588 OS=Cucumis melo OX=3656 GN=LOC103501588 PE=4 SV=1)

HSP 1 Score: 1323.5 bits (3424), Expect = 0.0e+00
Identity = 671/768 (87.37%), Postives = 710/768 (92.45%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHSPLLELPFQHSAETLTRPQLKALVLSRFS 60
           M IH RKIAT LKPK S S NKLYS LPL+   +L LP QHS E+LT  QLKALVLSRFS
Sbjct: 1   MFIHFRKIATSLKPKFSNSLNKLYSHLPLR-KKVLNLPSQHSPESLTSSQLKALVLSRFS 60

Query: 61  HGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNCFSVQDMARELY 120
           HGKF DL QNVVASPSVLLTAS+NLITPP +   NA DSLP+ D VS CFSV+ MAREL 
Sbjct: 61  HGKFVDLFQNVVASPSVLLTASRNLITPPFS---NAPDSLPLSDLVSKCFSVEVMARELS 120

Query: 121 QNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180
           +NRFD+GACCV MAP  EKG+SLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWF VAF RKKF+SVHVN LCLL+QEKI+DDILI+ML+KLFE+
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEV 240

Query: 241 GAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNVSF 300
            AIQIELG CYLGRGFPQESGLCSIL NIYFNGFDKEIQ+IRLQKNEENPKF+ D+ VSF
Sbjct: 241 EAIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSF 300

Query: 301 HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKI 360
           HNPVKIYAVRY DEILVITSGSKMLTMELKSQVLKYLEGNLELEVDR+NTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
            FLGMELQAV PSVLHPPMSEKAIRA+KKYLRQKEVRAIELRNARERNRKKLGLKILSH+
Sbjct: 361 GFLGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHM 420

Query: 421 FKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHI 480
           FKK K+T GFKSEFQIE EVR IFRNW+DEVVQDFFES EDHAEW+R L+ G FLSLKHI
Sbjct: 421 FKKFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDHAEWHRVLSAGDFLSLKHI 480

Query: 481 RNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCI 540
           RNQLPEDLVNAYDRFQ QV+KHLNPVK+KKEKAREDEEKR EEEE YAKRTVEDLTRLCI
Sbjct: 481 RNQLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCI 540

Query: 541 KVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KVDAPIELVRKAV+MVGFTNKMGRP+PI SL+ALED DIIKWY+GVGRRWLDFFCCCHNY
Sbjct: 541 KVDAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKML 660
           KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHY KDLKVFDLNGNEEMHFPTE+ VKML
Sbjct: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKML 660

Query: 661 GDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 720
           G+RNL+DPYPVDGALSL LIRLATDEASYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+G
Sbjct: 661 GERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           VEWV+GMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCT SLDMD
Sbjct: 721 VEWVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

BLAST of Tan0018751 vs. ExPASy TrEMBL
Match: A0A5A7SWY5 (RNA-directed DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold400G00320 PE=4 SV=1)

HSP 1 Score: 1320.8 bits (3417), Expect = 0.0e+00
Identity = 671/768 (87.37%), Postives = 709/768 (92.32%), Query Frame = 0

Query: 1   MLIHLRKIATPLKPKLSTSFNKLYSDLPLKHSPLLELPFQHSAETLTRPQLKALVLSRFS 60
           M IH RKIAT LKPK S S NKLYS LPL+   +L LP QHS E+LT  QLKALVLSRFS
Sbjct: 1   MFIHFRKIATSLKPKFSNSLNKLYSHLPLR-KKVLNLPSQHSPESLTSSQLKALVLSRFS 60

Query: 61  HGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNCFSVQDMARELY 120
           HGKF DL QNVVASPSVLLTAS+NLITPP +   NA DSLP+ D VS CFSV+ MAREL 
Sbjct: 61  HGKFVDLFQNVVASPSVLLTASRNLITPPFS---NAPDSLPLSDLVSKCFSVEVMARELS 120

Query: 121 QNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180
           +NRFD+GACCV MAP  EKG+SLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM
Sbjct: 121 ENRFDVGACCVPMAPLEEKGESLVLPNLKLKVLIEAIRMVLEIVYDERFVTFSYGGRVGM 180

Query: 181 GRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDILIFMLKKLFEL 240
           GRHTAIRYLKNSVQNPSWWF VAF RKKF+SVHVN LCLL+QEKI+DDILI+ML+KLFE+
Sbjct: 181 GRHTAIRYLKNSVQNPSWWFTVAFRRKKFESVHVNTLCLLMQEKIKDDILIYMLRKLFEV 240

Query: 241 GAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQIRLQKNEENPKFSPDDNVSF 300
            AIQIELG CYLGRGFPQESGLCSIL NIYFNGFDKEIQ+IRLQKNEENPKF+ D+ VSF
Sbjct: 241 EAIQIELGGCYLGRGFPQESGLCSILLNIYFNGFDKEIQRIRLQKNEENPKFNLDEIVSF 300

Query: 301 HNPVKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKI 360
           HNPVKIYAVRY DEILVITSGSKMLTMELKSQVLKYLEGNLELEVDR+NTAIHSAVSEKI
Sbjct: 301 HNPVKIYAVRYLDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRMNTAIHSAVSEKI 360

Query: 361 SFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420
            FLGMELQAV PSVLHPPMSEKAIRA+KKYLRQKEVRAIELRNARERNRKKLGLKILSHV
Sbjct: 361 GFLGMELQAVTPSVLHPPMSEKAIRARKKYLRQKEVRAIELRNARERNRKKLGLKILSHV 420

Query: 421 FKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHI 480
           FKK K+T GFKSEFQIE EVR IFRNW+DEVVQDFFES ED AEW+R L+ G FLSLKHI
Sbjct: 421 FKKFKRTGGFKSEFQIETEVRSIFRNWADEVVQDFFESSEDPAEWHRVLSAGDFLSLKHI 480

Query: 481 RNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCI 540
           RNQLPEDLVNAYDRFQ QV+KHLNPVK+KKEKAREDEEKR EEEE YAKRTVEDLTRLCI
Sbjct: 481 RNQLPEDLVNAYDRFQYQVNKHLNPVKVKKEKAREDEEKRLEEEELYAKRTVEDLTRLCI 540

Query: 541 KVDAPIELVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNY 600
           KVDAPIELVRKAV+MVGFTNKMGRP+PI SL+ALED DIIKWY+GVGRRWLDFFCCCHNY
Sbjct: 541 KVDAPIELVRKAVRMVGFTNKMGRPRPISSLIALEDADIIKWYSGVGRRWLDFFCCCHNY 600

Query: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKML 660
           KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHY KDLKVFDLNGNEEMHFPTE+ VKML
Sbjct: 601 KMVKTVVTYHLRFSCILTLAEKHESTKREAMKHYGKDLKVFDLNGNEEMHFPTEKAVKML 660

Query: 661 GDRNLSDPYPVDGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNG 720
           G+RNL+DPYPVDGALSL LIRLATDEASYPCIA+FCNRT+SILYRVRLLQRTLNVNPS+G
Sbjct: 661 GERNLADPYPVDGALSLLLIRLATDEASYPCIANFCNRTNSILYRVRLLQRTLNVNPSDG 720

Query: 721 VEWVRGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTSSLDMD 769
           VEWV+GMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCT SLDMD
Sbjct: 721 VEWVKGMGVIHESLNQRCLPLCADHISDLYMGKINLQDLDCTLSLDMD 764

BLAST of Tan0018751 vs. TAIR 10
Match: AT5G04050.2 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 750.4 bits (1936), Expect = 1.5e-216
Identity = 411/739 (55.62%), Postives = 505/739 (68.34%), Query Frame = 0

Query: 39  FQHSAETLTRP----QLKALVLSRFSHGKFFDLLQNVVASPSVLLTASQNLITPPPTNRL 98
           F +S +T+T P    +L+ALVL ++SHGKF+ L++N V+ P VLL A QNL        L
Sbjct: 33  FLNSDQTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNL-------SL 92

Query: 99  NALDSLPILDRVSNCFSVQDMARELYQNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLI 158
           +A  S  + DRVS  FS+++M RE+ + RFD+ +CCV    S     SLVLPNLKLKVLI
Sbjct: 93  SANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISS-----SLVLPNLKLKVLI 152

Query: 159 EAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHV 218
           EAIRMVLEIVYD+RF TFSYGGRVGMGRHTAIRYLKNSV+NP WWF V+F R+ F+  +V
Sbjct: 153 EAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNV 212

Query: 219 NKLCLLVQEKIEDDILIFMLKKLFELGAIQIELGACYLGRGFPQESGLCSILFNIYFNGF 278
           + LC  V EKI D +LI M+KKLFE G ++IELG C  GRGFPQE GLCSIL N+YF+G 
Sbjct: 213 DILCGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGL 272

Query: 279 DKEIQQIRLQKNEENPKFSPDDNVS----FHNPVKIYAVRYFDEILVITSGSKMLTMELK 338
           DKEIQ +RL+   +NP+    D  S    F  PV IYAVRY DEILVITSGSKMLTM+LK
Sbjct: 273 DKEIQDLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDLK 332

Query: 339 SQVLKYLEGNLELEVDRLNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRAQKKY 398
            +++  LE  LEL VDRLNT+IHSAVSEKI+FLGM LQAVPPSVL PP SEKA+RA KKY
Sbjct: 333 KRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKKY 392

Query: 399 LRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTDGFKSEFQIEKEVREIFRNWSDE 458
            RQK+VR +ELRNARERNRK LGLKI  HV KK+KQ++GFK E +IE EVR+IF++W +E
Sbjct: 393 QRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGEE 452

Query: 459 VVQDFFESLEDHAEWYRALTTGYFLSLKHIRNQLPEDLVNAYDRFQEQVDKHLNPVKIKK 518
           V+QDF  SLE+  +W+  LT G FLSL+HIR +LP+DL++AYD FQEQVDKHL P + K 
Sbjct: 453 VMQDFMGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK- 512

Query: 519 EKAREDEEKRAEEEEQ--YAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPQPI 578
            K  EDEE+R EEEE+  YA+RTVEDLT+LC+KV AP ELVRKA+               
Sbjct: 513 -KVLEDEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAI--------------- 572

Query: 579 CSLVALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTVVTYHLRFSCILTLAEKHESTKR 638
                                                                       
Sbjct: 573 ------------------------------------------------------------ 632

Query: 639 EAMKHYSKDLKVFDLNGNEEMHFPTEREVKMLGDRNLSDPYPVDGALSLFLIRLATDEAS 698
                     KV DL+G EE HFP+EREVKM+GD+NLSDP PVDG LSL LIRLA+DE  
Sbjct: 633 ----------KVSDLDGREEAHFPSEREVKMMGDKNLSDPKPVDGTLSLLLIRLASDEPL 672

Query: 699 YPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGVEWVRGMGVIHESLNQRCLPLCADHISD 758
           + C A FC R+D+I++RV LLQ  L++NP +  +WV GMG IH +LN++CLPLC+ HISD
Sbjct: 693 HHCAASFCERSDTIMHRVHLLQNRLHINPLDEEKWVPGMGTIHSALNRKCLPLCSTHISD 672

Query: 759 LYMGKINLQDLDCTSSLDM 768
           +Y+GKI LQD+D +S +D+
Sbjct: 753 VYLGKITLQDVDSSSFIDL 672

BLAST of Tan0018751 vs. TAIR 10
Match: AT5G04050.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 716.1 bits (1847), Expect = 3.1e-206
Identity = 375/579 (64.77%), Postives = 451/579 (77.89%), Query Frame = 0

Query: 39  FQHSAETLTRP----QLKALVLSRFSHGKFFDLLQNVVASPSVLLTASQNLITPPPTNRL 98
           F +S +T+T P    +L+ALVL ++SHGKF+ L++N V+ P VLL A QNL        L
Sbjct: 33  FLNSDQTITEPLVKSELEALVLKQYSHGKFYSLVKNAVSLPCVLLAACQNL-------SL 92

Query: 99  NALDSLPILDRVSNCFSVQDMARELYQNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLI 158
           +A  S  + DRVS  FS+++M RE+ + RFD+ +CCV    S     SLVLPNLKLKVLI
Sbjct: 93  SANSSGDLADRVSRRFSIEEMGREIREGRFDIRSCCVEFISS-----SLVLPNLKLKVLI 152

Query: 159 EAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHV 218
           EAIRMVLEIVYD+RF TFSYGGRVGMGRHTAIRYLKNSV+NP WWF V+F R+ F+  +V
Sbjct: 153 EAIRMVLEIVYDDRFATFSYGGRVGMGRHTAIRYLKNSVENPRWWFRVSFAREMFEERNV 212

Query: 219 NKLCLLVQEKIEDDILIFMLKKLFELGAIQIELGACYLGRGFPQESGLCSILFNIYFNGF 278
           + LC  V EKI D +LI M+KKLFE G ++IELG C  GRGFPQE GLCSIL N+YF+G 
Sbjct: 213 DILCGFVGEKINDVMLIEMIKKLFEFGILKIELGGCNSGRGFPQECGLCSILINVYFDGL 272

Query: 279 DKEIQQIRLQKNEENPKFSPDDNVS----FHNPVKIYAVRYFDEILVITSGSKMLTMELK 338
           DKEIQ +RL+   +NP+    D  S    F  PV IYAVRY DEILVITSGSKMLTM+LK
Sbjct: 273 DKEIQDLRLKMKVKNPRVGTGDEESTGNVFFKPVNIYAVRYLDEILVITSGSKMLTMDLK 332

Query: 339 SQVLKYLEGNLELEVDRLNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKAIRAQKKY 398
            +++  LE  LEL VDRLNT+IHSAVSEKI+FLGM LQAVPPSVL PP SEKA+RA KKY
Sbjct: 333 KRIVDILEQRLELRVDRLNTSIHSAVSEKINFLGMYLQAVPPSVLRPPKSEKAVRAMKKY 392

Query: 399 LRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTDGFKSEFQIEKEVREIFRNWSDE 458
            RQK+VR +ELRNARERNRK LGLKI  HV KK+KQ++GFK E +IE EVR+IF++W +E
Sbjct: 393 QRQKDVRKLELRNARERNRKTLGLKIFRHVLKKIKQSNGFKFEGEIENEVRDIFQSWGEE 452

Query: 459 VVQDFFESLEDHAEWYRALTTGYFLSLKHIRNQLPEDLVNAYDRFQEQVDKHLNPVKIKK 518
           V+QDF  SLE+  +W+  LT G FLSL+HIR +LP+DL++AYD FQEQVDKHL P + K 
Sbjct: 453 VMQDFMGSLEERWKWHWLLTRGDFLSLRHIREKLPQDLIDAYDEFQEQVDKHLAPTQAK- 512

Query: 519 EKAREDEEKRAEEEEQ--YAKRTVEDLTRLCIKVDAPIELVRKAVKMVGFTNKMGRPQPI 578
            K  EDEE+R EEEE+  YA+RTVEDLT+LC+KV AP ELVRKA+K+VGFTN MGRP+PI
Sbjct: 513 -KVLEDEERRVEEEEEQRYAERTVEDLTKLCMKVSAPEELVRKAIKLVGFTNSMGRPRPI 572

Query: 579 CSLVALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTVV 608
             LV LED+DIIKWYAGVGR+WLDFFCCCHNYKMVK++V
Sbjct: 573 IHLVTLEDSDIIKWYAGVGRKWLDFFCCCHNYKMVKSLV 597

BLAST of Tan0018751 vs. TAIR 10
Match: AT1G74350.1 (Intron maturase, type II family protein )

HSP 1 Score: 217.2 bits (552), Expect = 4.5e-56
Identity = 189/756 (25.00%), Postives = 329/756 (43.52%), Query Frame = 0

Query: 50  QLKALVLSRFSHGKFFDLLQNVVASPSVLLTASQNLITPPPTNRLNALDSLPILDRVSNC 109
           +LK  V  +  +GKF DLL+ V+A P  L  A   +       RLN+  ++ I +R +  
Sbjct: 45  RLKKRVKEQCINGKFSDLLKKVIARPETLRDAYDCI-------RLNS--NVSITER-NGS 104

Query: 110 FSVQDMARELYQNRFDLGACCVRMAPSGEKGQSLVLPNLKLKVLIEAIRMVLEIVYDERF 169
            +   +A EL    FD+ +    +    +  + LVLP++ LKV+ EAIR+VLE+V+   F
Sbjct: 105 VAFDSIAEELSSGVFDVASNTFSIVARDKTKEVLVLPSVALKVVQEAIRIVLEVVFSPHF 164

Query: 170 VTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFAVAFHRKKFDSVHVNKLCLLVQEKIEDDI 229
              S+  R G GR +A++Y+ N++    W F ++ ++K   SV  N L ++ +EK+ED  
Sbjct: 165 SKISHSCRSGRGRASALKYINNNISRSDWCFTLSLNKKLDVSVFENLLSVM-EEKVEDSS 224

Query: 230 LIFMLKKLFELGAIQIELGACYLGRGFPQESGLCSILFNIYFNGFDKEIQQI-------- 289
           L  +L+ +FE   + +E G    G G PQE  L  +L NIY + FD E  +I        
Sbjct: 225 LSILLRSMFEARVLNLEFGGFPKGHGLPQEGVLSRVLMNIYLDRFDHEFYRISMRHEALG 284

Query: 290 ------------------RLQKNEENPKFSPDDNVSFHNPVKIYAVRYFDEILVITSGSK 349
                             R Q  E+  K + + +V+    +++Y  R+ DEI    SG K
Sbjct: 285 LDSKTDEDSPGSKLRSWFRRQAGEQGLKSTTEQDVA----LRVYCCRFMDEIYFSVSGPK 344

Query: 350 MLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSEKISFLGMELQAVPPSVLHPPMSEKA 409
            +  +++S+ + +L  +L L++           +  +  LG     V  +V   P     
Sbjct: 345 KVASDIRSEAIGFLRNSLHLDITDETDPSPCEATSGLRVLG---TLVRKNVRESP----T 404

Query: 410 IRAQKKYLRQKEVRAIELRNARERNRKKLGLKILSHVFKKLKQTD--------------- 469
           ++A  K   +  + A++   A      ++G K L H  KK+K+++               
Sbjct: 405 VKAVHKLKEKVRLFALQKEEAWTLGTVRIGKKWLGHGLKKVKESEIKGLADSNSTLSQIS 464

Query: 470 -GFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYFLSLKHIRNQLPED 529
              K+  + +   + + R W ++V++   +  E+           + LS   +   +P++
Sbjct: 465 CHRKAGMETDHWYKILLRIWMEDVLRTSADRSEE-----------FVLSKHVVEPTVPQE 524

Query: 530 LVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVEDLTRLCIKVDAPIE 589
           L +A+ +FQ     +++              + A  E         D       V AP  
Sbjct: 525 LRDAFYKFQNAAAAYVS-------------SETANLEALLPCPQSHDRPVFFGDVVAPTN 584

Query: 590 LVRKAVKMVGFTNKMGRPQPICSLVALEDTDIIKWYAGVGRRWLDFFCCCHNYKMVKTVV 649
            + + +   G     G  +    L+ L+   II WY+G+ RRW+ ++  C N+  +K ++
Sbjct: 585 AIGRRLYRYGLITAKGYARSNSMLILLDTAQIIDWYSGLVRRWVIWYEGCSNFDEIKALI 644

Query: 650 TYHLRFSCILTLAEKHESTKREAMKHYSKDLKVFDLNGNEEMHFPTEREVKMLGDRNLSD 709
              +R SCI TLA K+   + E  K    +L       + E     E+      DR+   
Sbjct: 645 DNQIRMSCIRTLAAKYRIHENEIEKRLDLELSTIPSAEDIEQEIQHEKLDSPAFDRDEHL 704

Query: 710 PYPV--DGALSLFLIRLATDEASYPCIAHFCNRTDSILYRVRLLQRTLNVNPSNGVEWVR 761
            Y +   G   L L RL ++     C    C+     +Y +  ++R           W  
Sbjct: 705 TYGLSNSGLCLLSLARLVSESRPCNCFVIGCSMAAPAVYTLHAMER------QKFPGWKT 748

BLAST of Tan0018751 vs. TAIR 10
Match: ATMG00520.1 (Intron maturase, type II family protein )

HSP 1 Score: 73.6 bits (179), Expect = 8.0e-13
Identity = 143/670 (21.34%), Postives = 246/670 (36.72%), Query Frame = 0

Query: 151 KVLIEAIRMVLEIVYDERFVTFSYGGRVGMGRHTAIRYLKNSVQNPSWWFAVAFHRKKFD 210
           K++ EAIRMVLE +YD  F   S+  R G G H+ +R +K      S WF     RK F 
Sbjct: 15  KIMKEAIRMVLESIYDPEFPDTSH-FRSGQGCHSVLRRIKEE-WGISRWFLEFDIRKCFH 74

Query: 211 SVHVNKLCLLVQEKIEDDILIFMLKKLFELG-AIQIELGACYLGRGFPQESGLCSILFNI 270
           ++  ++L  +++E+I+D    + ++K+F  G  + +E G        P    L ++  NI
Sbjct: 75  TIDRHRLIQILKEEIDDPKFFYSIQKVFSAGRLVGVERGP----YSVPHSVLLSALPGNI 134

Query: 271 YFNGFDKEIQQIRLQ--------------------KNEENPK-----FSPDDN------- 330
           Y +  D+EI +IR +                     ++ENP       +P DN       
Sbjct: 135 YLHKLDQEIGRIRQKYEIPIVQRVRSVLLRTGRRIDDQENPGEEASFNAPQDNRAIIVGS 194

Query: 331 --------------VSFHNP---------------------------------------- 390
                          S+H P                                        
Sbjct: 195 VKSMQRKAAFHSLVSSWHTPPTSTLRLRGDQKRPFVFPPSSALAVFLNKPSSLLCAAFLI 254

Query: 391 ------------------------------------------------------------ 450
                                                                       
Sbjct: 255 EAAGLTPKAEFYGGERCNNNWAMRDLLKYCKRKGLLIELGGEAILVIRSERGLARKQAPL 314

Query: 451 -----VKIYAVRYFDEILVITSGSKMLTMELKSQVLKYLEGNLELEVDRLNTAIHSAVSE 510
                ++I   RY D++L+   G+  L +E++ ++  +L+  L L V    +   +A S 
Sbjct: 315 KTHYLIRICYARYADDLLLGIVGAVELLIEIQKRIAHFLQSGLNLWVGSAGSTTIAARS- 374

Query: 511 KISFLGMELQAVPPSVLHPPMSEKAIRAQKKYLRQK---EVRAIELRNARERNRKKLGLK 570
            + FLG  ++ VPP     P+  + +R  +K LR K    + A  LR+A     + LG  
Sbjct: 375 TVEFLGTVIREVPPRT--TPI--QFLRELEKRLRVKHRIHITACHLRSAIHSKFRNLGDS 434

Query: 571 I-LSHVFKKLKQTDGFKSEFQIEKEVREIFRNWSDEVVQDFFESLEDHAEWYRALTTGYF 630
           I +  + K + +T   +   Q+ + +       +  V       L    +  R  + G  
Sbjct: 435 IPIKQLTKGMSKTGSLQDGVQLAETL------GTAGVRSPQVSVLWGTVKHIRQGSRGIS 494

Query: 631 LSLKHIRNQLPEDLVNAYDRFQEQVDKHLNPVKIKKEKAREDEEKRAEEEEQYAKRTVED 662
                 R+    D+     R       H   + +     R    K A E   +   ++  
Sbjct: 495 FLHSSGRSNASSDVQQVVSR----SGTHARKLSLYTPPGR----KAAGEGGGHWAGSIS- 554