Tan0018253 (gene) Snake gourd v1

Overview
NameTan0018253
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionprotein EDS1L-like
LocationLG09: 71961095 .. 71966012 (-)
RNA-Seq ExpressionTan0018253
SyntenyTan0018253
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGAAAGTACCTGGATATTTATTTATTGATTCCTTCACATAAAGTTTCTGAGAAGCCTCCAATCAAAAACAACGTTTTGGAATTGAAAAACGACAAGTAACTGTGAAAAGGCATCCAATAATTTATCATATATGTCGAAGACGAAGACGAAGAAGACTTCGTCAAGCTGAAGCTGAAGCAGGGCAGAGCAGAGAGCATCATCGGAGCTGAGGAAAATGGTTCACGCAAGGCTTGAGGAGGCCACTGGTTTAAAGGCAGAGCTCATCGGAAGCGCCTGGTCGGCCGCCATTAAAGCTCACAAGTATTCGGAGAAGCCATTTCTTCTTGAGAATACCAGAGACTTTTCTATCATCAGTTTCGCCGGATCATGGTCGGTGGAAAGCTGGTTCTCTGGTTCCGATTCATCTTTTGGAGAAACCAAGATCAACACTCAGCTGTTTCCGTCCATCAGGAGCATCGGCGTTGACGATTATGCGCAGGTCAATTCTGCATTTCTCCAGAGATTTGAAGGGATTTTGGGGAAGCTGATCAAGGAGGTACTTTGCCCTAATTTTCCCCCAATTTGTTCTTTCTCCGTTCTTCCGTATTTTATGGCTCTACTGCTTTAATTATTATATTCTTCACATTTGCCTTTTCTTGGATACGGATAATTTCAGTTATTCCTCTTTTTAACTTACAATTCTCTATTTATTTACACCTTTTTCAGTTTTTTTTTTTTTTTTAATTTTTAAAATTTTAGTCTCTCACAGTTGGTCAAATTTAAATTTGGTTATCCAATCTCAAAAATTCTTTGTCACACCAAACTAAATCATCGGCTAGGAAATTGAACTCGTAAACGATAGTTAATCAAAATTAAAGTTGACTTGTATGCGTATGTGACATCATACTGAGTGAGTATATATAGCAGAAATTTAGAAGAGGAAAAATGTAAAAAAATTATTTATTTTAGATGACTTTTTTCCATCTATATTTCTACAATAAATTAAGTATTATACTATAGTTTATCATTAACCAACTTTAACAGGGATGAAGACAAAATTAAAAAGGTTAATTCATACACTACTAAATTGAAAAGATATACCGATATTAAAGAGTGAAACATGTATTTAACCACAAATTAACTATAAATGGTCTAATAAAAATGTATTTTCTAAATATGTTAGCAATTTCAATTTGCAATCATGGTATATTATATAAAATTTTAAATATAATGTTTAAGATGTTGGCATATTTTAAACTTTGTTATCTATCGAAGTAAAAATATACCTTCTAAAGTATAATATATTATTTTTGTAATATATGAATTTATGATGGACCAATATTAATAGATAAGGATAGACGGTTAAAAGTAAGGATATCAATTACTCTCCACTAAAAGGTTAGAGGTTAAAATTCTCTCTTCAAATATTGTTGTTGACCTAAAAAAAAAATAAAAAAATAAAAAAAGGAAAAAAGGAAAAAGAAAAAGAAATAATGCTTTGCCTAAAGGTGTCAAAATCTAGCATGTTTGATTCATATTTCATAAACCATTTATGAGAAACCATTTTTAGAAATTGTATTTTTACTCCTCCAAGACTCATTATTGTTGGCCCTAAAGTTATGAGTCTAAAGTATCTAATAAGTCCTTAAATTTTATCAATTTTGTACCTAATAAGTCTTTGATATATTCAAACTTTTAAAAAATTTGTTGATCTATCTGTTGAGGATTGCACAATAGAAAAGTGGAGAGAAACCTTCAATATATAAGATATATGAGCTACTTCTCTCATAGCCAATTAGTTTTGAGATAGAGCCTCATATAATCTAATATAGTATCAGAGCCCATTAAACTCAAATGGATACTTGATCCAAAAAATTGAAATTGGGATTCGATCCAAGAATGGTGAACCTAAAGAGACCCCATCTTGAAAGGGCATGTTTAGGATCCCACATTGGAAAAGTGGAGAGAAATCTCACAATATATATATACGATATATGGGATATTCCTCTCATTGTGAATTGGTTTTGAGATGGAACCCCATATAATCTAATACTATCAAATATAAAATTAAATTTTGTGTAACAAAACTCTAAAATTTCAATTTCGTCTCTAATAGCTTTGTGAATTTTATTGTCAAGAACTTATTAGATACAACATAAAAAAAATTTAAGGACTTTTTTTTATATATAATAAAGTTTAAGAATTTATTGGACTTTTTTTTAGTTTAAAGATCTATTAGATATAAAATTGAAAGTTGAAGAATAAACAAGCTTAAATAGTTTAGCTACTTAATTTTTCTGAGACCCCTTGCTTCTTTTAACTATAACCGGGTTTTGTTTCTCAGGTTCTGCGGGTGAATAAACCAGTAGTGTTTACTGGCCACTCTGCTGGAGGTCCAATAGCCATTCTTGCAACCATTTGGCTATTGGAAGAGCAAAGAAAAAACTCAAACAAAGACATCAACTCCACTCCTCCAAAATGCATCACATTTGGGTCTCCCCTTGTTGGTAACTTCATTCTCTCCCATGCTCTCAAAAGAGAGAAATGGTCGATTCATTTCGTACACTTCGTCATGCGATACGACATTGTCCCACGAATCCATCTCGCTCCTCTACCGTCCTTGCAACCAGTACAGCTGCAAACCATCCTCAACTCCTTGAACTCGAGATCACTCGGGTCGTCGTCGAGTGGGAATGTAGCTACTGCGTTCTTCATGACGGTGTTGAGTAATGCGTCGACTGTGGCGAACAATGCAGCCTGCAATCTTATGGGAAACACTAGCCTTTTGCTAGACAATTTGAAAAGCTTTATCAAATTGAGTCCTTATAGCCCTTTTGGGACTTACATTTTCTGTAGTGAGAGTGAGAAAGTGGTGGTGGTGACCAATCCAGATGCTGTTTTACAAATATTATTCTACTCTTGTCAGTTGAACTCTGAGAGAGAATGTGGTCAGATAGCTCATCAGAGTTTAAAGGACCATTGGGAGTATGAATCTAAAATGCAGCAAAACTTGGAGCTCCTGAATGCTATTCGTCTTGATGAATTGGTTAAGCTTCCTCTGTCTTTGACTGGAAGGAGCACGCCAATCACAGAAGCCTTGAATCAACTTGGCCTAGTAAGTTTTTTAATCTTCTTGCTCCCTCTCTCTTTTTCTTAAGCCTCTTTTTTATTTTATGTTCTAAGAATCATGCATTTCCATGTGTATCTTCTTCAACTCTTTCATTTGTATCCATTTGGTTTAGAAGGGCTTGATTTTGGAGGTGATTTGGTTATAAGATCAACTTTTAAAGCTAAATTATAGAAAATACTCATGAACTTTGCCGCTTGTTTTAAACATATCCCTATTCTTTCAAAAGTTGCAATATCACTTTTAGCCTTTCATAAACGTTTCAAAACTACCCTTAGAGTAGAAATCCTTTAGGGGACGTTTGGAGCACCATTTCGGAATCAAGACCCTTGGAGTCAGAAACCTAGGGTTTGAAAGCCAGTAAATTAAAAAATAGTATTTGGAGTGCATTGTCCAAATCATGGGGCTATATTTCAAACCCATTGGATTTTAAACACGGTGGCCCAATCAACGTTTTGGGTTTGGATTCAAAACTATCCAAACTCAACGTGGTGCTCCAAATGTTGCCTTAGAATGTGGCGATGACTTAAAATGACATGACTATGACTAAGATACTCGTCTTGACTAAATCTAAAAGGTTAAATTAGAACGACATTTAGATACGTTAATTTTTCGTTCAATCATTTAACGGGTTCTTTCTGCTCCAAAGGTAGTTTTGAAACATCTATAAAAGATTAGTAGGTAATATTACAACTTTTGAAAGAATCGAAGTAGTTTTGAAACAAGAGGCGAAAGCCATCTGATTTTATAGTCTCTTATCTTGGAATAGTTTGAAATGTCATACGGTCCATGACATATGGGAAGCATCAACATATCAATAAAGTGGTTGACCTGCCATGGATTCTAATTTTCATGAATGTGAAACTACAGAGTAGTACAAGGGCTTTATTGAATCTTCGAGCAGCCGGCGCATTCGAAGAACAGAAGATGAAAAACCAAGAAAGGATGGAAGTAAAGAAGAAATCAATCGAGGAAGGATTGAATTGGCTACAAGAAAACTACAGAGCTGTATGTAAGGTTGATGGATTGGGATATTATGATGCATTTAAACTTCAAAAGAATGACAAAGACTTCAAAGCTAATATCAAAAGGCTTGAACAAGCAGGTATATGGGATGAGATCTTAGGAATCCTCAAAATGTATGAACTCCCAGATGAATTCGAAGGCAACGACGAATGGATTCGACTCGGAACGAAGTTTCGCCGTCTCGTTGAGCCTCTGGATATTGCTAATTACTATAGACACTCTAAGAATGATGACACTGGTCCTTATTTGAAACAAGGAAGGCCGAATCGTTACAGATTTACACAAAGATGGCTCGAACATGATCGAAAGTTGAGAGAGTACAGTGAAGAATCGATTCTTTGGGCAATGGTTGAGGAGCTTCAAATCCAAACCAGAACAAGAATGTATGCAGAATTTTCAGGTGAAATTATAGAGCTGGAGAAGAAGATGAAGCGATGGATCAATGAGATTGAAGATGATATGTTGTTAGAGAAATCTACGTTCATGGAGTGGTGGAAAGCACTTCCTGAGCAACACAGATTACAGTCTTGTATTAAGGATGATATTGAGAGGATGAAGAATAGAGAGGATGCTATCAATTGGGAGTAGTCTAAAGAAATTATTCGATGAGACCGTTACAATAAAGCCGGAAAATTGTGAAAGTATCTTGCTTTTGTATTTAGTGATTATTAAGGGTAAACAAATTTAAGATCTATATCGGAAGATTTTAAATGGCTTCCAATTGAATAATTTATCAGTAATAGTGTGATTTTAATGAGCAATCGAATTCTTAGTTTGATTTTTT

mRNA sequence

GTGGAAAGTACCTGGATATTTATTTATTGATTCCTTCACATAAAGTTTCTGAGAAGCCTCCAATCAAAAACAACGTTTTGGAATTGAAAAACGACAAGTAACTGTGAAAAGGCATCCAATAATTTATCATATATGTCGAAGACGAAGACGAAGAAGACTTCGTCAAGCTGAAGCTGAAGCAGGGCAGAGCAGAGAGCATCATCGGAGCTGAGGAAAATGGTTCACGCAAGGCTTGAGGAGGCCACTGGTTTAAAGGCAGAGCTCATCGGAAGCGCCTGGTCGGCCGCCATTAAAGCTCACAAGTATTCGGAGAAGCCATTTCTTCTTGAGAATACCAGAGACTTTTCTATCATCAGTTTCGCCGGATCATGGTCGGTGGAAAGCTGGTTCTCTGGTTCCGATTCATCTTTTGGAGAAACCAAGATCAACACTCAGCTGTTTCCGTCCATCAGGAGCATCGGCGTTGACGATTATGCGCAGGTCAATTCTGCATTTCTCCAGAGATTTGAAGGGATTTTGGGGAAGCTGATCAAGGAGGTTCTGCGGGTGAATAAACCAGTAGTGTTTACTGGCCACTCTGCTGGAGGTCCAATAGCCATTCTTGCAACCATTTGGCTATTGGAAGAGCAAAGAAAAAACTCAAACAAAGACATCAACTCCACTCCTCCAAAATGCATCACATTTGGGTCTCCCCTTGTTGGTAACTTCATTCTCTCCCATGCTCTCAAAAGAGAGAAATGGTCGATTCATTTCGTACACTTCGTCATGCGATACGACATTGTCCCACGAATCCATCTCGCTCCTCTACCGTCCTTGCAACCAGTACAGCTGCAAACCATCCTCAACTCCTTGAACTCGAGATCACTCGGGTCGTCGTCGAGTGGGAATGTAGCTACTGCGTTCTTCATGACGGTGTTGAGTAATGCGTCGACTGTGGCGAACAATGCAGCCTGCAATCTTATGGGAAACACTAGCCTTTTGCTAGACAATTTGAAAAGCTTTATCAAATTGAGTCCTTATAGCCCTTTTGGGACTTACATTTTCTGTAGTGAGAGTGAGAAAGTGGTGGTGGTGACCAATCCAGATGCTGTTTTACAAATATTATTCTACTCTTGTCAGTTGAACTCTGAGAGAGAATGTGGTCAGATAGCTCATCAGAGTTTAAAGGACCATTGGGAGTATGAATCTAAAATGCAGCAAAACTTGGAGCTCCTGAATGCTATTCGTCTTGATGAATTGGTTAAGCTTCCTCTGTCTTTGACTGGAAGGAGCACGCCAATCACAGAAGCCTTGAATCAACTTGGCCTAAGTAGTACAAGGGCTTTATTGAATCTTCGAGCAGCCGGCGCATTCGAAGAACAGAAGATGAAAAACCAAGAAAGGATGGAAGTAAAGAAGAAATCAATCGAGGAAGGATTGAATTGGCTACAAGAAAACTACAGAGCTGTATGTAAGGTTGATGGATTGGGATATTATGATGCATTTAAACTTCAAAAGAATGACAAAGACTTCAAAGCTAATATCAAAAGGCTTGAACAAGCAGGTATATGGGATGAGATCTTAGGAATCCTCAAAATGTATGAACTCCCAGATGAATTCGAAGGCAACGACGAATGGATTCGACTCGGAACGAAGTTTCGCCGTCTCGTTGAGCCTCTGGATATTGCTAATTACTATAGACACTCTAAGAATGATGACACTGGTCCTTATTTGAAACAAGGAAGGCCGAATCGTTACAGATTTACACAAAGATGGCTCGAACATGATCGAAAGTTGAGAGAGTACAGTGAAGAATCGATTCTTTGGGCAATGGTTGAGGAGCTTCAAATCCAAACCAGAACAAGAATGTATGCAGAATTTTCAGGTGAAATTATAGAGCTGGAGAAGAAGATGAAGCGATGGATCAATGAGATTGAAGATGATATGTTGTTAGAGAAATCTACGTTCATGGAGTGGTGGAAAGCACTTCCTGAGCAACACAGATTACAGTCTTGTATTAAGGATGATATTGAGAGGATGAAGAATAGAGAGGATGCTATCAATTGGGAGTAGTCTAAAGAAATTATTCGATGAGACCGTTACAATAAAGCCGGAAAATTGTGAAAGTATCTTGCTTTTGTATTTAGTGATTATTAAGGGTAAACAAATTTAAGATCTATATCGGAAGATTTTAAATGGCTTCCAATTGAATAATTTATCAGTAATAGTGTGATTTTAATGAGCAATCGAATTCTTAGTTTGATTTTTT

Coding sequence (CDS)

ATGGTTCACGCAAGGCTTGAGGAGGCCACTGGTTTAAAGGCAGAGCTCATCGGAAGCGCCTGGTCGGCCGCCATTAAAGCTCACAAGTATTCGGAGAAGCCATTTCTTCTTGAGAATACCAGAGACTTTTCTATCATCAGTTTCGCCGGATCATGGTCGGTGGAAAGCTGGTTCTCTGGTTCCGATTCATCTTTTGGAGAAACCAAGATCAACACTCAGCTGTTTCCGTCCATCAGGAGCATCGGCGTTGACGATTATGCGCAGGTCAATTCTGCATTTCTCCAGAGATTTGAAGGGATTTTGGGGAAGCTGATCAAGGAGGTTCTGCGGGTGAATAAACCAGTAGTGTTTACTGGCCACTCTGCTGGAGGTCCAATAGCCATTCTTGCAACCATTTGGCTATTGGAAGAGCAAAGAAAAAACTCAAACAAAGACATCAACTCCACTCCTCCAAAATGCATCACATTTGGGTCTCCCCTTGTTGGTAACTTCATTCTCTCCCATGCTCTCAAAAGAGAGAAATGGTCGATTCATTTCGTACACTTCGTCATGCGATACGACATTGTCCCACGAATCCATCTCGCTCCTCTACCGTCCTTGCAACCAGTACAGCTGCAAACCATCCTCAACTCCTTGAACTCGAGATCACTCGGGTCGTCGTCGAGTGGGAATGTAGCTACTGCGTTCTTCATGACGGTGTTGAGTAATGCGTCGACTGTGGCGAACAATGCAGCCTGCAATCTTATGGGAAACACTAGCCTTTTGCTAGACAATTTGAAAAGCTTTATCAAATTGAGTCCTTATAGCCCTTTTGGGACTTACATTTTCTGTAGTGAGAGTGAGAAAGTGGTGGTGGTGACCAATCCAGATGCTGTTTTACAAATATTATTCTACTCTTGTCAGTTGAACTCTGAGAGAGAATGTGGTCAGATAGCTCATCAGAGTTTAAAGGACCATTGGGAGTATGAATCTAAAATGCAGCAAAACTTGGAGCTCCTGAATGCTATTCGTCTTGATGAATTGGTTAAGCTTCCTCTGTCTTTGACTGGAAGGAGCACGCCAATCACAGAAGCCTTGAATCAACTTGGCCTAAGTAGTACAAGGGCTTTATTGAATCTTCGAGCAGCCGGCGCATTCGAAGAACAGAAGATGAAAAACCAAGAAAGGATGGAAGTAAAGAAGAAATCAATCGAGGAAGGATTGAATTGGCTACAAGAAAACTACAGAGCTGTATGTAAGGTTGATGGATTGGGATATTATGATGCATTTAAACTTCAAAAGAATGACAAAGACTTCAAAGCTAATATCAAAAGGCTTGAACAAGCAGGTATATGGGATGAGATCTTAGGAATCCTCAAAATGTATGAACTCCCAGATGAATTCGAAGGCAACGACGAATGGATTCGACTCGGAACGAAGTTTCGCCGTCTCGTTGAGCCTCTGGATATTGCTAATTACTATAGACACTCTAAGAATGATGACACTGGTCCTTATTTGAAACAAGGAAGGCCGAATCGTTACAGATTTACACAAAGATGGCTCGAACATGATCGAAAGTTGAGAGAGTACAGTGAAGAATCGATTCTTTGGGCAATGGTTGAGGAGCTTCAAATCCAAACCAGAACAAGAATGTATGCAGAATTTTCAGGTGAAATTATAGAGCTGGAGAAGAAGATGAAGCGATGGATCAATGAGATTGAAGATGATATGTTGTTAGAGAAATCTACGTTCATGGAGTGGTGGAAAGCACTTCCTGAGCAACACAGATTACAGTCTTGTATTAAGGATGATATTGAGAGGATGAAGAATAGAGAGGATGCTATCAATTGGGAGTAG

Protein sequence

MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLENTRDFSIISFAGSWSVESWFSGSDSSFGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPVVFTGHSAGGPIAILATIWLLEEQRKNSNKDINSTPPKCITFGSPLVGNFILSHALKREKWSIHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQILFYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPITEALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDGLGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRRLVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILWAMVEELQIQTRTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRLQSCIKDDIERMKNREDAINWE
Homology
BLAST of Tan0018253 vs. ExPASy Swiss-Prot
Match: Q9XF23 (Protein EDS1L OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.8e-116
Identity = 255/636 (40.09%), Postives = 375/636 (58.96%), Query Frame = 0

Query: 7   EEATGLKAELIGSAWSAAIKAHKYSEKPFLLENTRDFSIISFAGSWSVESWFS-GSDSSF 66
           E  TG+  +LI  +WSA+ +A  Y  + +  E      I +F  S+S + +F   + SSF
Sbjct: 4   EALTGINGDLITRSWSASKQA--YLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSF 63

Query: 67  GETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILG------KLIKEVLRVNKPVVFTG 126
           GE K+N   FP +R IG  D A VN AFL+  E ++         ++  +R  K +VFTG
Sbjct: 64  GEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAVIDPRTSFQASVEMAVRSRKQIVFTG 123

Query: 127 HSAGGPIAILATIWLLEEQ-RKNSNKDINSTPPKCITFGSPLVGNFILSHALKREKWSIH 186
           HS+GG  AILAT+W LE+   +N N  +    P+C+TFG+PLVG+ I SHAL REKWS  
Sbjct: 124 HSSGGATAILATVWYLEKYFIRNPNVYLE---PRCVTFGAPLVGDSIFSHALGREKWSRF 183

Query: 187 FVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNAS 246
           FV+FV R+DIVPRI LA   S++   L  +L  L+ R+     S    T F+ +V+ + S
Sbjct: 184 FVNFVTRFDIVPRITLARKASVEET-LPHVLAQLDPRNSSVQESEQRITEFYTSVMRDTS 243

Query: 247 TVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQILFY 306
           TVAN A C L G+   +L+ L SF++LSPY P GT++F +E +++V V N DA+LQ+LFY
Sbjct: 244 TVANQAVCELTGSAEAILETLSSFLELSPYRPAGTFVFSTE-KRLVAVNNSDAILQMLFY 303

Query: 307 SCQLNSERECGQIAHQSLKDHWEYESKMQ-QNLELLNAIRLDELVKLPLSLTGRSTPITE 366
           +CQ + E+E   I  +S++DH  YE  +Q   ++L N             L G ++ I  
Sbjct: 304 TCQASDEQEWSLIPFRSIRDHHSYEELVQSMGMKLFN------------HLDGENS-IES 363

Query: 367 ALNQLGLSSTRALLNLRAAGAFEEQKMKNQERM--EVKKKSIEEGLNWLQENYRAVCKVD 426
           +LN LG+ STR    ++AA   E+++++NQ+++   ++++   + L W+++ Y+  C+  
Sbjct: 364 SLNDLGV-STRGRQYVQAALEEEKKRVENQKKIIQVIQQERFLKKLAWIEDEYKPKCQAH 423

Query: 427 GLGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFR 486
             GYYD+FK+   + DFKAN+KR E AG++DE+LG+LK  +LPDEFEG+ +WI+L T++R
Sbjct: 424 KNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLLKKCQLPDEFEGDIDWIKLATRYR 483

Query: 487 RLVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEH------------------- 546
           RLVEPLDIANY+RH KN+DTGPY+K+GRP RY + QR  EH                   
Sbjct: 484 RLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHHILKPNGMIAEDVFWNKVN 543

Query: 547 ----DRKLREYSEE---------SILWAMVEELQIQTRTRMYAEFSGEIIELEKKMKRWI 597
                 +L E  E          S  WA VEEL    + + Y E    +  LE  ++ WI
Sbjct: 544 GLNLGLQLEEIQETLKNSGSECGSCFWAEVEEL----KGKPYEEVEVRVKTLEGMLREWI 603

BLAST of Tan0018253 vs. ExPASy Swiss-Prot
Match: Q9SU72 (Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 4.8e-114
Identity = 254/635 (40.00%), Postives = 369/635 (58.11%), Query Frame = 0

Query: 7   EEATGLKAELIGSAWSAAIKAHKYSEKPFLLENTRDFSIISFAGSWSVESWFS-GSDSSF 66
           E  TG+  +LI  +WSA+ +A  Y  + +  E      I +F  S+S + +F   + SSF
Sbjct: 4   EALTGINGDLITRSWSASKQA--YLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSF 63

Query: 67  GETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILG------KLIKEVLRVNKPVVFTG 126
           GE K+N   FP +R IG  D A VN AFL+  E I+         ++  +R  K +VFTG
Sbjct: 64  GEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFTG 123

Query: 127 HSAGGPIAILATIWLLEEQ-RKNSNKDINSTPPKCITFGSPLVGNFILSHALKREKWSIH 186
           HS+GG  AILAT+W LE+   +N N  +    P+C+TFG+PLVG+ I SHAL REKWS  
Sbjct: 124 HSSGGATAILATVWYLEKYFIRNPNVYLE---PRCVTFGAPLVGDSIFSHALGREKWSRF 183

Query: 187 FVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNAS 246
           FV+FV R+DIVPRI LA   S++   L  +L  L+ R      S    T F+  V+ + S
Sbjct: 184 FVNFVSRFDIVPRIMLARKASVEET-LPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTS 243

Query: 247 TVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQILFY 306
           TVAN A C L G+    L+ L SF++LSPY P GT++F +E +++V V N DA+LQ+LFY
Sbjct: 244 TVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTE-KRLVAVNNSDAILQMLFY 303

Query: 307 SCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPITEA 366
           + Q + E+E   I  +S++DH  YE  +Q   +           KL   L G ++ I   
Sbjct: 304 TSQASDEQEWSLIPFRSIRDHHSYEELVQSMGK-----------KLFNHLDGENS-IEST 363

Query: 367 LNQLGLSSTRALLNLRAAGAFEEQKMKNQERM--EVKKKSIEEGLNWLQENYRAVCKVDG 426
           LN LG+ STR    ++AA   E+++++NQ+++   ++++   + L W+++ Y+  C+   
Sbjct: 364 LNDLGV-STRGRQYVQAALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHK 423

Query: 427 LGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRR 486
            GYYD+FK+   + DFKAN+KR E AG++DE+LG++K  +LPDEFEG+ +WI+L T++RR
Sbjct: 424 NGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRR 483

Query: 487 LVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEH-------------------- 546
           LVEPLDIANY+RH KN+DTGPY+K+GRP RY + QR  EH                    
Sbjct: 484 LVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNG 543

Query: 547 ---DRKLREYSEE---------SILWAMVEELQIQTRTRMYAEFSGEIIELEKKMKRWI- 597
                +L E  E          S  WA VEEL    + + Y E    +  LE  +  WI 
Sbjct: 544 LNLGLQLEEIQETLKNSGSECGSCFWAEVEEL----KGKPYEEVEVRVKTLEGMLGEWIT 603

BLAST of Tan0018253 vs. ExPASy Swiss-Prot
Match: Q9SU71 (Protein EDS1B OS=Arabidopsis thaliana OX=3702 GN=EDS1B PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 4.5e-112
Identity = 253/629 (40.22%), Postives = 361/629 (57.39%), Query Frame = 0

Query: 7   EEATGLKAELIGSAWSAAIKAHKYSEKPFLLENTRDFSIISFAGSWSVESWFSGSDSS-F 66
           E  TG+  +L+  +W A+  A++   + +L E        +F  S+S E  F+  ++S F
Sbjct: 4   EALTGVNGDLVTISWMASKGANQ--TEHYLKEEVGGTVFFAFRASFSSEDLFATENTSPF 63

Query: 67  GETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILG------KLIKEVLRVNKPVVFTG 126
           GE K+    FP +RSIG D    VN AFL+  E ++G        ++  +   + VVFTG
Sbjct: 64  GEIKMKRNQFPCMRSIGNDVDTTVNEAFLKSLEVLIGPRTSFHASVQSAVDRKQQVVFTG 123

Query: 127 HSAGGPIAILATIWLLEEQRKNSNKDINSTP-PKCITFGSPLVGNFILSHALKREKWSIH 186
           HS GG  AILAT+W LE       +D  + P P+C+TFG+PLVG++I  HAL RE WS  
Sbjct: 124 HSFGGATAILATVWYLETY---FIRDAYAAPEPRCVTFGAPLVGDYIFKHALGRENWSRF 183

Query: 187 FVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNAS 246
           FV+FV R+DIVPRI LA   +++   L  +L  L+S       S  V T F+  V+ +  
Sbjct: 184 FVNFVTRFDIVPRIMLARKTTIEQT-LSYVLGKLDSTRAPIHESDQVITEFYTRVMRDTY 243

Query: 247 TVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQILFY 306
           TVA+ A C L+GN    L+ L SF +LSPY P GT++F S  +++VVV N DA+LQ+LFY
Sbjct: 244 TVASKAVCQLIGNGEAFLETLSSFYELSPYRPVGTFVF-STQKRLVVVNNSDAILQMLFY 303

Query: 307 SCQLNSERECGQIAHQSLKDHWEYESKMQQ-NLELLNAIRLDELVKLPLSLTGRSTPITE 366
           +CQ N E+E   I   S++DH  YE  +Q   ++LLN + L      PL L G ++ I  
Sbjct: 304 TCQSNDEQELSVIPFLSIRDHHGYEELVQSIGIKLLNHLDLHN----PL-LDGENS-IGS 363

Query: 367 ALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDGL 426
           AL+ LG+ STRA   + AA   E+Q+++NQ+++E K+  I E L W+ E Y+  C+    
Sbjct: 364 ALDDLGM-STRARQCIHAALEAEKQRVENQKKIETKRDQIVERLTWIVEVYKPKCQAHKN 423

Query: 427 GYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRRL 486
           GYYD+FK    + DFKAN+KR+E AGI+DE+LG++K  +LPD FEG+  WI L T++RRL
Sbjct: 424 GYYDSFKDSNEENDFKANVKRVELAGIFDEVLGLVKKGQLPDGFEGSRGWINLATQYRRL 483

Query: 487 VEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHD-------------------- 546
           +EPLDI+NY+   KN+DTGPY+  GRP+RY++ QR  EHD                    
Sbjct: 484 IEPLDISNYHGQLKNEDTGPYMLHGRPSRYKYAQRGYEHDILKPTGMIAKDVFWSKVNGL 543

Query: 547 ---------RKLREYSEE--SILWAMVEELQIQTRTRMYAEFSGEIIELEKKMKRWINEI 593
                      L+    E  S  WA VEEL    + + Y E       LE  ++ WI + 
Sbjct: 544 NLGLQQDIQEILKNSGSECGSCFWAEVEEL----KGKPYEEVQVRFKTLEGLLEGWIKDG 603

BLAST of Tan0018253 vs. ExPASy Swiss-Prot
Match: Q9S745 (Lipase-like PAD4 OS=Arabidopsis thaliana OX=3702 GN=PAD4 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.1e-25
Identity = 123/486 (25.31%), Postives = 189/486 (38.89%), Query Frame = 0

Query: 74  LFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRV----NKPVVFTGHSAGGPIAIL 133
           LFP + S   +    V++A L+ F   L   IKE L +     K VV TGHS GG +A  
Sbjct: 72  LFPGLSS--DEPLPMVDAAILKLF---LQLKIKEGLELELLGKKLVVITGHSTGGALAAF 131

Query: 134 ATIWLLEEQRKNSNKDINSTPPK----CITFGSPLVGNFILSHALKREKWSIHFVHFVMR 193
             +WLL +          S+PP     CITFGSPL+GN  LS ++ R + + +F H V  
Sbjct: 132 TALWLLSQ----------SSPPSFRVFCITFGSPLLGNQSLSTSISRSRLAHNFCHVVSI 191

Query: 194 YDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNASTVANNAA 253
           +D+VPR                              S N                     
Sbjct: 192 HDLVPR------------------------------SSN--------------------- 251

Query: 254 CNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVV--VTNPDAVLQILFYSCQLN 313
                                 + PFGTY+FCS+   V +    +   +  IL  +   N
Sbjct: 252 --------------------EQFWPFGTYLFCSDKGGVCLDNAGSVRLMFNILNTTATQN 311

Query: 314 SERE------CGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPITE 373
           +E           ++H  LK        +  N          E   L  S    S  + +
Sbjct: 312 TEEHQRYGHYVFTLSHMFLKSRSFLGGSIPDNSYQAGVALAVE--ALGFSNDDTSGVLVK 371

Query: 374 ALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDGL 433
              +      RA + LR+A    E       R+E++         W ++  R     + L
Sbjct: 372 ECIETATRIVRAPI-LRSAELANELASVLPARLEIQ---------WYKD--RCDASEEQL 431

Query: 434 GYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRRL 493
           GYYD FK     +DFK N+ R+  A  WD ++ +++  ELP +F    +WI     ++ L
Sbjct: 432 GYYDFFKRYSLKRDFKVNMSRIRLAKFWDTVIKMVETNELPFDFHLGKKWIYASQFYQLL 457

Query: 494 VEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDR--------KLREYSEESIL 536
            EPLDIAN+Y++      G YL+  RP RY    +W +  +        +    ++++  
Sbjct: 492 AEPLDIANFYKNRDIKTGGHYLEGNRPKRYEVIDKWQKGVKVPEECVRSRYASTTQDTCF 457

BLAST of Tan0018253 vs. ExPASy Swiss-Prot
Match: Q4F883 (Senescence-associated carboxylesterase 101 OS=Arabidopsis thaliana OX=3702 GN=SAG101 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.3e-22
Identity = 143/624 (22.92%), Postives = 231/624 (37.02%), Query Frame = 0

Query: 16  LIGSAWSAAIKAH-----------------KYSEKPFLLENTRDFSIISFAGSWSVESWF 75
           L+ S+WS  ++ H                 K S   F +     F+++ F+      S  
Sbjct: 21  LLHSSWSKILEIHNPPYSNHDPGLQVSKKKKDSGLEFQIHREEKFTLVVFSAPPICRS-- 80

Query: 76  SGSDSSFGETKINTQLFPSIRSIGVDDYAQVNSAF-----LQRFEGILGKLIKEVLRVNK 135
           S SDS+    K     FP + S     ++    AF            L   + + L+  K
Sbjct: 81  SSSDSTLLHVKDKENPFPFLCSENNPSFSLHTPAFNLFTSASTSLTYLKSELLQTLKSEK 140

Query: 136 PVVFTGHSAGGPIAILATIWLLEEQRKNSNKDINSTPPKCITFGSPLVGNFILSHALKRE 195
           PV+ TG + GG +A L T+WLLE       +      P CITFGSPL+G+  L   L+  
Sbjct: 141 PVIITGAALGGSVASLYTLWLLETIEPTLKR------PLCITFGSPLIGDASLQQILENS 200

Query: 196 KWSIHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTV 255
             +  F+H V                                                  
Sbjct: 201 VRNSCFLHVV-------------------------------------------------- 260

Query: 256 LSNASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVL 315
                                   + ++ IK+  + PFGT++ C +S   V + +  AV 
Sbjct: 261 ------------------------SAQTRIKMDFFKPFGTFLICFDS-GCVCIEDHVAVT 320

Query: 316 QILFYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRL--DELVKLPLSLTGR 375
           ++      LN   + G      L D+ +  +++ Q++  L   RL  ++++K        
Sbjct: 321 EL------LNGVHDSG------LVDYSQVLNRLDQSMLSLADSRLIPEDVIK-------- 380

Query: 376 STPITEALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAV 435
                      G+     + NLR      +   K    M++    IE    W    Y+  
Sbjct: 381 -----------GIEKRAEMKNLRF-----DMMFKKLNDMKISMAYIE----W----YKKK 440

Query: 436 CKVDGLGYYDAFKLQK--NDKDFKANIK---RLEQAGIWDEILGILKMYELPDEFEGNDE 495
           CK   +GYYD FK Q     K+F  NIK   + E    W  ++  ++     D       
Sbjct: 441 CKEVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASILKRR 500

Query: 496 WIRLGTKFRRLVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRW-------LEHDR- 555
           ++  G  +RR++EPLDIA YY   + +    Y   GR + Y   ++W       +E +R 
Sbjct: 501 FLFSGNNYRRMIEPLDIAEYYLEGRKE----YRTTGRSHHYVMLEKWFGMESILIEKERC 508

Query: 556 KLREYSE----ESILWAMVEELQI----------------QTRTRMYAEFSGEIIELEKK 583
           K R+ S+    +S  WA VE+  I                +  TR   EF G + E+  K
Sbjct: 561 KKRDLSDLLTFDSCFWAEVEDSLIVINQLNTTVGMRDDVREVLTRKLVEFEGYVWEIITK 508

BLAST of Tan0018253 vs. NCBI nr
Match: XP_023529901.1 (protein EDS1L-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.3e-302
Identity = 519/615 (84.39%), Postives = 572/615 (93.01%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLE-NTRDFSIISFAGSWSVESWFS 60
           MVHARLE+ATG KAELIGSAW+AA+KAH+Y+EKPFL+E ++RDFSIISF GSWS E WFS
Sbjct: 1   MVHARLEDATGFKAELIGSAWTAAVKAHRYAEKPFLVETSSRDFSIISFPGSWSAEGWFS 60

Query: 61  GSDSSFGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPVVFTG 120
           GSDS FGETKINTQLFPSIRSIGV+DYA VNSAFL RFE ILGKLIKEV+RV+K VVFTG
Sbjct: 61  GSDSCFGETKINTQLFPSIRSIGVNDYALVNSAFLHRFEAILGKLIKEVMRVSKAVVFTG 120

Query: 121 HSAGGPIAILATIWLLEEQRKNSNKDI---NSTPPKCITFGSPLVGNFILSHALKREKWS 180
           HSAGGPIAILATIWLLEEQRKNSN +    +STPPKCITFGSPLVGNFILSHALKREKWS
Sbjct: 121 HSAGGPIAILATIWLLEEQRKNSNSNTDTNSSTPPKCITFGSPLVGNFILSHALKREKWS 180

Query: 181 IHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSN 240
           IHF+HF+MRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSL SSS+G+V TAFF TV+SN
Sbjct: 181 IHFIHFIMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLESSSNGDVTTAFFTTVMSN 240

Query: 241 ASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQIL 300
           AS+V NNAAC+LM +TSLLL+NL+SFIKLSPYSPFGTYIFC+ES++VVV+TNP AVLQIL
Sbjct: 241 ASSVTNNAACHLMESTSLLLENLRSFIKLSPYSPFGTYIFCTESDEVVVLTNPHAVLQIL 300

Query: 301 FYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPIT 360
           FYSCQLNSE E GQ+AHQSLKDHWEYESKM QNLEL +A+RLDEL KLPLSLTGRSTPIT
Sbjct: 301 FYSCQLNSESERGQVAHQSLKDHWEYESKMHQNLELQHAVRLDELHKLPLSLTGRSTPIT 360

Query: 361 EALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDG 420
           +ALN+LGL STRALLNLRAAG +EE+K KNQERME KK+ IE+GLNWL+ENYRAVC+VDG
Sbjct: 361 QALNRLGL-STRALLNLRAAGTYEEKKTKNQERMEAKKQYIEQGLNWLEENYRAVCRVDG 420

Query: 421 LGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRR 480
           +GYYDAFK QKND DFKANIKRLEQAGIWDEILG+LKMYELPDEFEGNDEWI+LGTK+RR
Sbjct: 421 MGYYDAFKRQKNDTDFKANIKRLEQAGIWDEILGMLKMYELPDEFEGNDEWIQLGTKYRR 480

Query: 481 LVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILWAMVEEL 540
           LVEPLDIANYYRHSKNDDTGPYLK GRPNRYRFTQRWLEH+RK+ EYSEESILWAMVEEL
Sbjct: 481 LVEPLDIANYYRHSKNDDTGPYLKMGRPNRYRFTQRWLEHNRKMTEYSEESILWAMVEEL 540

Query: 541 QIQTRTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRLQSCIKD 600
           QIQT+TRMY E S EIIELEKKMKRW+NEI +DMLL+KSTFMEWWK LPEQHR QSC+ D
Sbjct: 541 QIQTQTRMYVEHSREIIELEKKMKRWVNEIGEDMLLKKSTFMEWWKTLPEQHRSQSCLMD 600

Query: 601 DIERMKNREDAINWE 612
           DI+RM+ R+DA+NWE
Sbjct: 601 DIQRMEKRDDAMNWE 614

BLAST of Tan0018253 vs. NCBI nr
Match: XP_023529896.1 (protein EDS1L-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529897.1 protein EDS1L-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529898.1 protein EDS1L-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529899.1 protein EDS1L-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529900.1 protein EDS1L-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.3e-302
Identity = 519/615 (84.39%), Postives = 572/615 (93.01%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLE-NTRDFSIISFAGSWSVESWFS 60
           MVHARLE+ATG KAELIGSAW+AA+KAH+Y+EKPFL+E ++RDFSIISF GSWS E WFS
Sbjct: 39  MVHARLEDATGFKAELIGSAWTAAVKAHRYAEKPFLVETSSRDFSIISFPGSWSAEGWFS 98

Query: 61  GSDSSFGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPVVFTG 120
           GSDS FGETKINTQLFPSIRSIGV+DYA VNSAFL RFE ILGKLIKEV+RV+K VVFTG
Sbjct: 99  GSDSCFGETKINTQLFPSIRSIGVNDYALVNSAFLHRFEAILGKLIKEVMRVSKAVVFTG 158

Query: 121 HSAGGPIAILATIWLLEEQRKNSNKDI---NSTPPKCITFGSPLVGNFILSHALKREKWS 180
           HSAGGPIAILATIWLLEEQRKNSN +    +STPPKCITFGSPLVGNFILSHALKREKWS
Sbjct: 159 HSAGGPIAILATIWLLEEQRKNSNSNTDTNSSTPPKCITFGSPLVGNFILSHALKREKWS 218

Query: 181 IHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSN 240
           IHF+HF+MRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSL SSS+G+V TAFF TV+SN
Sbjct: 219 IHFIHFIMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLESSSNGDVTTAFFTTVMSN 278

Query: 241 ASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQIL 300
           AS+V NNAAC+LM +TSLLL+NL+SFIKLSPYSPFGTYIFC+ES++VVV+TNP AVLQIL
Sbjct: 279 ASSVTNNAACHLMESTSLLLENLRSFIKLSPYSPFGTYIFCTESDEVVVLTNPHAVLQIL 338

Query: 301 FYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPIT 360
           FYSCQLNSE E GQ+AHQSLKDHWEYESKM QNLEL +A+RLDEL KLPLSLTGRSTPIT
Sbjct: 339 FYSCQLNSESERGQVAHQSLKDHWEYESKMHQNLELQHAVRLDELHKLPLSLTGRSTPIT 398

Query: 361 EALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDG 420
           +ALN+LGL STRALLNLRAAG +EE+K KNQERME KK+ IE+GLNWL+ENYRAVC+VDG
Sbjct: 399 QALNRLGL-STRALLNLRAAGTYEEKKTKNQERMEAKKQYIEQGLNWLEENYRAVCRVDG 458

Query: 421 LGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRR 480
           +GYYDAFK QKND DFKANIKRLEQAGIWDEILG+LKMYELPDEFEGNDEWI+LGTK+RR
Sbjct: 459 MGYYDAFKRQKNDTDFKANIKRLEQAGIWDEILGMLKMYELPDEFEGNDEWIQLGTKYRR 518

Query: 481 LVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILWAMVEEL 540
           LVEPLDIANYYRHSKNDDTGPYLK GRPNRYRFTQRWLEH+RK+ EYSEESILWAMVEEL
Sbjct: 519 LVEPLDIANYYRHSKNDDTGPYLKMGRPNRYRFTQRWLEHNRKMTEYSEESILWAMVEEL 578

Query: 541 QIQTRTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRLQSCIKD 600
           QIQT+TRMY E S EIIELEKKMKRW+NEI +DMLL+KSTFMEWWK LPEQHR QSC+ D
Sbjct: 579 QIQTQTRMYVEHSREIIELEKKMKRWVNEIGEDMLLKKSTFMEWWKTLPEQHRSQSCLMD 638

Query: 601 DIERMKNREDAINWE 612
           DI+RM+ R+DA+NWE
Sbjct: 639 DIQRMEKRDDAMNWE 652

BLAST of Tan0018253 vs. NCBI nr
Match: XP_022972365.1 (protein EDS1L-like [Cucurbita maxima] >XP_022972376.1 protein EDS1L-like [Cucurbita maxima])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.3e-302
Identity = 524/621 (84.38%), Postives = 573/621 (92.27%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLE-NTRDFSIISFAGSWSVESWFS 60
           MVHARLE+ATG KAELIGSAW+AA+KAHKY+EKPFLLE ++RDFSIISF GSWS E WFS
Sbjct: 1   MVHARLEDATGFKAELIGSAWTAAVKAHKYAEKPFLLETSSRDFSIISFPGSWSAEGWFS 60

Query: 61  GSDSS----FGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPV 120
           GSDS     FGETKINTQLFPSIRSIGV+DYA VNSAFL RFE ILGKLIKEV+RV+K V
Sbjct: 61  GSDSDSDSCFGETKINTQLFPSIRSIGVNDYALVNSAFLHRFEAILGKLIKEVMRVSKAV 120

Query: 121 VFTGHSAGGPIAILATIWLLEEQRKNSNKDIN-----STPPKCITFGSPLVGNFILSHAL 180
           VFTGHSAGGPIAILATIWLLEEQRKNSN + N     STPPKCITFGSPLVGNFILSHAL
Sbjct: 121 VFTGHSAGGPIAILATIWLLEEQRKNSNSNSNTDTNSSTPPKCITFGSPLVGNFILSHAL 180

Query: 181 KREKWSIHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFF 240
           KREKWSIHF+HF+MRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSL SSS+G+V TAFF
Sbjct: 181 KREKWSIHFIHFIMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLESSSNGDVTTAFF 240

Query: 241 MTVLSNASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPD 300
           MTV+SNAS+VANNAAC+LM +TSLLLDNLKSFIKLSPYSPFGTYIFC+ES++VVV+TNP 
Sbjct: 241 MTVMSNASSVANNAACHLMESTSLLLDNLKSFIKLSPYSPFGTYIFCTESDEVVVLTNPH 300

Query: 301 AVLQILFYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTG 360
           AVLQILFYSCQLNSE E GQ+AHQSLKDHWEYESKM QNLELL+AIRLDEL KLPLSLTG
Sbjct: 301 AVLQILFYSCQLNSESEWGQVAHQSLKDHWEYESKMHQNLELLHAIRLDELHKLPLSLTG 360

Query: 361 RSTPITEALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRA 420
           RSTPIT+ALN+LGL STRALLNLRAAG +EE+KMKNQ RME K + IE+GLNWL+ENYRA
Sbjct: 361 RSTPITQALNRLGL-STRALLNLRAAGTYEEKKMKNQVRMEAKMQYIEQGLNWLEENYRA 420

Query: 421 VCKVDGLGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRL 480
           VC+VDG+GYYDAFK QKND DFKANIKRLEQ G+WDEILG+LKMY+LPDEFEGNDEWI+L
Sbjct: 421 VCRVDGMGYYDAFKRQKNDTDFKANIKRLEQTGLWDEILGMLKMYDLPDEFEGNDEWIQL 480

Query: 481 GTKFRRLVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILW 540
           GTK+RRLVEPLDIANYYRHSKNDDTGPYLK GRPNRYRFTQRWLEH++K+ EYSEESILW
Sbjct: 481 GTKYRRLVEPLDIANYYRHSKNDDTGPYLKMGRPNRYRFTQRWLEHNQKMTEYSEESILW 540

Query: 541 AMVEELQIQTRTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRL 600
           AMVEELQIQTRTRMY E S EIIELEKKMKRW+NEI +DMLL+KSTFMEWWK LPEQHR 
Sbjct: 541 AMVEELQIQTRTRMYVEHSREIIELEKKMKRWVNEIGEDMLLKKSTFMEWWKTLPEQHRS 600

Query: 601 QSCIKDDIERMKNREDAINWE 612
           QSC+KDDI+RM+ R+DA+ WE
Sbjct: 601 QSCLKDDIQRMEKRDDAMIWE 620

BLAST of Tan0018253 vs. NCBI nr
Match: KAG7021481.1 (Protein EDS1L, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.7e-302
Identity = 521/615 (84.72%), Postives = 573/615 (93.17%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLE-NTRDFSIISFAGSWSVESWFS 60
           MVHARLE+ATG KAELIGSAW+AA+KAHK++EKPFLLE ++RDFSIISF GSWS E WFS
Sbjct: 39  MVHARLEDATGFKAELIGSAWTAAVKAHKHAEKPFLLETSSRDFSIISFPGSWSAEGWFS 98

Query: 61  GSDSSFGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPVVFTG 120
           GSDS FGETKINTQLFPSIRSIGV+DYA VNSAFL RFE ILGKLIKEV+RV+K VVFTG
Sbjct: 99  GSDSCFGETKINTQLFPSIRSIGVNDYALVNSAFLHRFEAILGKLIKEVMRVSKAVVFTG 158

Query: 121 HSAGGPIAILATIWLLEEQRKNSNKDI---NSTPPKCITFGSPLVGNFILSHALKREKWS 180
           HSAGGPIAILATIWLLEEQRKNSN +    +STPPKCITFGSPLVGNFILSHALKREKWS
Sbjct: 159 HSAGGPIAILATIWLLEEQRKNSNSNTDTNSSTPPKCITFGSPLVGNFILSHALKREKWS 218

Query: 181 IHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSN 240
           IHF+HF+MRYD+VPRIHLAPLPSLQPVQLQTILNSLNSRSL SSS+G+V TAFFMTV+SN
Sbjct: 219 IHFIHFIMRYDVVPRIHLAPLPSLQPVQLQTILNSLNSRSLESSSNGDVTTAFFMTVMSN 278

Query: 241 ASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQIL 300
           AS+VANNAAC+LM +TSLLL+NL+SFIKLSPYSPFGTYIFC+ES++VVV+TNP AVLQIL
Sbjct: 279 ASSVANNAACHLMESTSLLLENLRSFIKLSPYSPFGTYIFCTESDEVVVLTNPHAVLQIL 338

Query: 301 FYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPIT 360
           FYSCQ NSE E GQ+AHQSLKDHWEYESKM QNLELL+AIRLDEL KLPLSLTGRSTPIT
Sbjct: 339 FYSCQFNSESERGQVAHQSLKDHWEYESKMHQNLELLHAIRLDELHKLPLSLTGRSTPIT 398

Query: 361 EALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDG 420
           +ALN+LGL STRALLNLRAAG +EE+K KNQERME KK+ IE+GLN L+ENYRAVC+VDG
Sbjct: 399 QALNRLGL-STRALLNLRAAGTYEEKKTKNQERMEAKKQYIEQGLNSLEENYRAVCRVDG 458

Query: 421 LGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRR 480
           +GYYDAFK QKND DFKANIKRLE AGIWDEILG+LKMYELPDEFEGNDEWI+LGTK+RR
Sbjct: 459 MGYYDAFKRQKNDTDFKANIKRLELAGIWDEILGMLKMYELPDEFEGNDEWIQLGTKYRR 518

Query: 481 LVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILWAMVEEL 540
           LVEPLDIANYYRHSKNDDTGPYLK GRPNRYRFTQRWLEH+RK+ EYSEESILWAMVEEL
Sbjct: 519 LVEPLDIANYYRHSKNDDTGPYLKMGRPNRYRFTQRWLEHNRKMTEYSEESILWAMVEEL 578

Query: 541 QIQTRTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRLQSCIKD 600
           QIQTRTRMY E S EIIELEKKMKRW+NEI +DMLL+KSTFMEWWK LPEQHR QSC+KD
Sbjct: 579 QIQTRTRMYVEHSREIIELEKKMKRWVNEIGEDMLLKKSTFMEWWKTLPEQHRSQSCLKD 638

Query: 601 DIERMKNREDAINWE 612
           DI+RM+ ++DA+NWE
Sbjct: 639 DIQRMEKKDDAMNWE 652

BLAST of Tan0018253 vs. NCBI nr
Match: KAG6587497.1 (Protein EDS1L, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1045.8 bits (2703), Expect = 1.4e-301
Identity = 522/616 (84.74%), Postives = 573/616 (93.02%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLE-NTRDFSIISFAGSWSVESWFS 60
           MVHARLE+ATG KAELIGSAW+AA+KAHK++EKPFLLE ++RDFSIISF GSWS E WFS
Sbjct: 39  MVHARLEDATGFKAELIGSAWTAAVKAHKHAEKPFLLETSSRDFSIISFPGSWSAEGWFS 98

Query: 61  GSDSSFGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPVVFTG 120
           GSDS FGETKINTQLFPSIRSIGV+DYA VNSAFL RFE ILGKLIKEV+RV+K VVFTG
Sbjct: 99  GSDSCFGETKINTQLFPSIRSIGVNDYALVNSAFLHRFEAILGKLIKEVMRVSKAVVFTG 158

Query: 121 HSAGGPIAILATIWLLEEQRKNSNKDI---NSTPPKCITFGSPLVGNFILSHALKREKWS 180
           HSAGGPIAILATIWLLEEQRKNSN +    +STPPKCITFGSPLVGNFILSHALKREKWS
Sbjct: 159 HSAGGPIAILATIWLLEEQRKNSNSNTDTNSSTPPKCITFGSPLVGNFILSHALKREKWS 218

Query: 181 IHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSN 240
           IHF+HF+MRYD+VPRIHLAPLPSLQPVQLQTILNSLNSRSL SSS+G+V TAFFMTV+SN
Sbjct: 219 IHFIHFIMRYDVVPRIHLAPLPSLQPVQLQTILNSLNSRSLESSSNGDVTTAFFMTVMSN 278

Query: 241 ASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQIL 300
           AS+VANNAAC+LM +TSLLL+NL+SFIKLSPYSPFGTYIFC+ES++VVV+TNP AVLQIL
Sbjct: 279 ASSVANNAACHLMESTSLLLENLRSFIKLSPYSPFGTYIFCTESDEVVVLTNPHAVLQIL 338

Query: 301 FYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPIT 360
           FYSCQ NSE E GQ+AHQSLKDHWEYESKM QNLELL+AIRLDEL KLPLSLTGRSTPIT
Sbjct: 339 FYSCQFNSESERGQVAHQSLKDHWEYESKMHQNLELLHAIRLDELHKLPLSLTGRSTPIT 398

Query: 361 EALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDG 420
           +ALNQLGL STRALLNLRAAG +EE+K KNQERME KK+ IE+GLN L+ENYRAVC+VDG
Sbjct: 399 QALNQLGL-STRALLNLRAAGTYEEKKTKNQERMEAKKQYIEQGLNSLEENYRAVCRVDG 458

Query: 421 LGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRR 480
           +GYYDAFK QKND DFKANIKRLE AGIWDEILG+LKMYELPDEFEGNDEWI+LGTK+RR
Sbjct: 459 MGYYDAFKRQKNDTDFKANIKRLELAGIWDEILGMLKMYELPDEFEGNDEWIQLGTKYRR 518

Query: 481 LVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREY-SEESILWAMVEE 540
           LVEPLDIANYYRHSKNDDTGPYLK GRPNRYRFTQRWLEH+RK+ EY SEESILWAMVEE
Sbjct: 519 LVEPLDIANYYRHSKNDDTGPYLKMGRPNRYRFTQRWLEHNRKMTEYSSEESILWAMVEE 578

Query: 541 LQIQTRTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRLQSCIK 600
           LQIQTRTRMY E S EIIELEKKMKRW+NEI +DMLL+KSTFMEWWK LPEQHR QSC+K
Sbjct: 579 LQIQTRTRMYVEHSREIIELEKKMKRWVNEIGEDMLLKKSTFMEWWKTLPEQHRSQSCLK 638

Query: 601 DDIERMKNREDAINWE 612
           DDI+RM+ ++DA+NWE
Sbjct: 639 DDIQRMEKKDDAMNWE 653

BLAST of Tan0018253 vs. ExPASy TrEMBL
Match: A0A6J1I8E2 (protein EDS1L-like OS=Cucurbita maxima OX=3661 GN=LOC111470937 PE=4 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 6.3e-303
Identity = 524/621 (84.38%), Postives = 573/621 (92.27%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLE-NTRDFSIISFAGSWSVESWFS 60
           MVHARLE+ATG KAELIGSAW+AA+KAHKY+EKPFLLE ++RDFSIISF GSWS E WFS
Sbjct: 1   MVHARLEDATGFKAELIGSAWTAAVKAHKYAEKPFLLETSSRDFSIISFPGSWSAEGWFS 60

Query: 61  GSDSS----FGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPV 120
           GSDS     FGETKINTQLFPSIRSIGV+DYA VNSAFL RFE ILGKLIKEV+RV+K V
Sbjct: 61  GSDSDSDSCFGETKINTQLFPSIRSIGVNDYALVNSAFLHRFEAILGKLIKEVMRVSKAV 120

Query: 121 VFTGHSAGGPIAILATIWLLEEQRKNSNKDIN-----STPPKCITFGSPLVGNFILSHAL 180
           VFTGHSAGGPIAILATIWLLEEQRKNSN + N     STPPKCITFGSPLVGNFILSHAL
Sbjct: 121 VFTGHSAGGPIAILATIWLLEEQRKNSNSNSNTDTNSSTPPKCITFGSPLVGNFILSHAL 180

Query: 181 KREKWSIHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFF 240
           KREKWSIHF+HF+MRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSL SSS+G+V TAFF
Sbjct: 181 KREKWSIHFIHFIMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLESSSNGDVTTAFF 240

Query: 241 MTVLSNASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPD 300
           MTV+SNAS+VANNAAC+LM +TSLLLDNLKSFIKLSPYSPFGTYIFC+ES++VVV+TNP 
Sbjct: 241 MTVMSNASSVANNAACHLMESTSLLLDNLKSFIKLSPYSPFGTYIFCTESDEVVVLTNPH 300

Query: 301 AVLQILFYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTG 360
           AVLQILFYSCQLNSE E GQ+AHQSLKDHWEYESKM QNLELL+AIRLDEL KLPLSLTG
Sbjct: 301 AVLQILFYSCQLNSESEWGQVAHQSLKDHWEYESKMHQNLELLHAIRLDELHKLPLSLTG 360

Query: 361 RSTPITEALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRA 420
           RSTPIT+ALN+LGL STRALLNLRAAG +EE+KMKNQ RME K + IE+GLNWL+ENYRA
Sbjct: 361 RSTPITQALNRLGL-STRALLNLRAAGTYEEKKMKNQVRMEAKMQYIEQGLNWLEENYRA 420

Query: 421 VCKVDGLGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRL 480
           VC+VDG+GYYDAFK QKND DFKANIKRLEQ G+WDEILG+LKMY+LPDEFEGNDEWI+L
Sbjct: 421 VCRVDGMGYYDAFKRQKNDTDFKANIKRLEQTGLWDEILGMLKMYDLPDEFEGNDEWIQL 480

Query: 481 GTKFRRLVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILW 540
           GTK+RRLVEPLDIANYYRHSKNDDTGPYLK GRPNRYRFTQRWLEH++K+ EYSEESILW
Sbjct: 481 GTKYRRLVEPLDIANYYRHSKNDDTGPYLKMGRPNRYRFTQRWLEHNQKMTEYSEESILW 540

Query: 541 AMVEELQIQTRTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRL 600
           AMVEELQIQTRTRMY E S EIIELEKKMKRW+NEI +DMLL+KSTFMEWWK LPEQHR 
Sbjct: 541 AMVEELQIQTRTRMYVEHSREIIELEKKMKRWVNEIGEDMLLKKSTFMEWWKTLPEQHRS 600

Query: 601 QSCIKDDIERMKNREDAINWE 612
           QSC+KDDI+RM+ R+DA+ WE
Sbjct: 601 QSCLKDDIQRMEKRDDAMIWE 620

BLAST of Tan0018253 vs. ExPASy TrEMBL
Match: A0A6J1F4K9 (protein EDS1L-like OS=Cucurbita moschata OX=3662 GN=LOC111440567 PE=4 SV=1)

HSP 1 Score: 1043.1 bits (2696), Expect = 4.5e-301
Identity = 522/619 (84.33%), Postives = 573/619 (92.57%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLE-NTRDFSIISFAGSWSVESWFS 60
           MVHARLE+ATG KAELIGSAW+AA+KAHKY+EKPFLLE ++RDFSIISF GSWS E WFS
Sbjct: 1   MVHARLEDATGFKAELIGSAWTAAVKAHKYAEKPFLLETSSRDFSIISFPGSWSAEGWFS 60

Query: 61  GSDSS----FGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPV 120
           GSDS     FGETKINTQLFPSIRSIGV+DYA VNSAFL RFE ILGKLIKEV+RV+K V
Sbjct: 61  GSDSDSDSCFGETKINTQLFPSIRSIGVNDYALVNSAFLHRFEAILGKLIKEVMRVSKAV 120

Query: 121 VFTGHSAGGPIAILATIWLLEEQRKNSNKDI---NSTPPKCITFGSPLVGNFILSHALKR 180
           VFTGHSAGGPIAILATIWLLEEQRKNSN +    +STPPKCITFGSPLVGNFILSHALKR
Sbjct: 121 VFTGHSAGGPIAILATIWLLEEQRKNSNSNTVTNSSTPPKCITFGSPLVGNFILSHALKR 180

Query: 181 EKWSIHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMT 240
           EKWSIHF+HF+MRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSL SSS+G+V TAFF T
Sbjct: 181 EKWSIHFIHFIMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLESSSNGDVTTAFFST 240

Query: 241 VLSNASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAV 300
           V+SNAS+VANNAAC+LM +TSLLL+NL+SFIKLSPYSPFGTYIFC+ES++VVV+TNP AV
Sbjct: 241 VMSNASSVANNAACHLMESTSLLLENLRSFIKLSPYSPFGTYIFCTESDEVVVLTNPHAV 300

Query: 301 LQILFYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRS 360
           LQILFYSCQLNS+ E GQ+AHQSLKDHWEYESKM QNLELL+AIRLDEL KLPLSLTGRS
Sbjct: 301 LQILFYSCQLNSDSERGQVAHQSLKDHWEYESKMHQNLELLHAIRLDELHKLPLSLTGRS 360

Query: 361 TPITEALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVC 420
           TPIT+ALN+LGL STRALLNLRAAG +EE+K KNQERME KK+ IE+GLN L+ENYRAVC
Sbjct: 361 TPITQALNRLGL-STRALLNLRAAGTYEEKKTKNQERMEAKKQYIEKGLNSLEENYRAVC 420

Query: 421 KVDGLGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGT 480
           +VDG+GYYDAFK QKND DFKANIKRLE AGIWDEILG+LKMYELPDEFEGNDEWI+LGT
Sbjct: 421 RVDGMGYYDAFKRQKNDTDFKANIKRLELAGIWDEILGMLKMYELPDEFEGNDEWIQLGT 480

Query: 481 KFRRLVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILWAM 540
           K+RRLVEPLDIANYYRHSKNDDTGPYLK GRPNRYRFTQRWLEH++K+ EYSEESILWAM
Sbjct: 481 KYRRLVEPLDIANYYRHSKNDDTGPYLKMGRPNRYRFTQRWLEHNQKMTEYSEESILWAM 540

Query: 541 VEELQIQTRTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRLQS 600
           VEELQIQTRTRMY E S EIIELEKKMKRW+NEI +DMLL+KSTFMEWWK LPEQHR QS
Sbjct: 541 VEELQIQTRTRMYVEHSREIIELEKKMKRWVNEIGEDMLLKKSTFMEWWKTLPEQHRSQS 600

Query: 601 CIKDDIERMKNREDAINWE 612
           C+KDDI+RM+ R+DA+NWE
Sbjct: 601 CLKDDIQRMEKRDDAMNWE 618

BLAST of Tan0018253 vs. ExPASy TrEMBL
Match: A0A6J1JLB1 (protein EDS1L-like OS=Cucurbita maxima OX=3661 GN=LOC111485621 PE=4 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 8.6e-284
Identity = 490/609 (80.46%), Postives = 553/609 (90.80%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLENTRDFSIISFAGSWSVESWFSG 60
           MVH+RLE+ATGLK ELIG+AWSAA+KAHK+++KPFLL+ TRDFSIISFAGSWS E+WFS 
Sbjct: 1   MVHSRLEDATGLKEELIGNAWSAAVKAHKHADKPFLLDKTRDFSIISFAGSWSPEAWFSS 60

Query: 61  SDSSFGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPVVFTGH 120
           SDSSFGETKIN QLFPS+RSIG+D+YA+VNSAF +RFE ILGKL KEVL+ NKPV FTGH
Sbjct: 61  SDSSFGETKINRQLFPSVRSIGLDEYAEVNSAFFRRFEAILGKL-KEVLKANKPVAFTGH 120

Query: 121 SAGGPIAILATIWLLEEQRKNSNKDINSTPPKCITFGSPLVGNFILSHALKREKWSIHFV 180
           SAGGP+AILATIWLLE+QR NSN + N TPPKCITFGSPLVGNFI SHALKREKWSIHFV
Sbjct: 121 SAGGPVAILATIWLLEQQR-NSNTNTNFTPPKCITFGSPLVGNFIFSHALKREKWSIHFV 180

Query: 181 HFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNASTV 240
           HFV RYDIVPRIHLAPLPSLQP  LQTIL SLNSRSLGS+S+GNVAT FFMTV+ NAS V
Sbjct: 181 HFVTRYDIVPRIHLAPLPSLQP-HLQTILTSLNSRSLGSASNGNVATEFFMTVMRNASAV 240

Query: 241 ANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQILFYSC 300
           A+NAAC+LMGNT+LLLD LKSF+KLSPYSPFGTYIF ++SEKVVVVTNPDAVLQILF++C
Sbjct: 241 ASNAACHLMGNTNLLLDTLKSFVKLSPYSPFGTYIFFTDSEKVVVVTNPDAVLQILFFAC 300

Query: 301 QLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPITEALN 360
           QL+SE EC  IAHQSLK HWEYESKM+QNLELL+AIRLDEL KLPLSLTGR+TPIT+ALN
Sbjct: 301 QLSSESECDNIAHQSLKAHWEYESKMRQNLELLHAIRLDELYKLPLSLTGRNTPITQALN 360

Query: 361 QLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDGLGYY 420
           +LGLSS RALLNLRAAG++EEQK++NQERM +KK+ IE+ LNWL+ENYR VCKVDGLGYY
Sbjct: 361 ELGLSS-RALLNLRAAGSYEEQKIRNQERMSLKKQDIEKLLNWLEENYRDVCKVDGLGYY 420

Query: 421 DAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRRLVEP 480
           DAFKLQK+ KDF+ANIKRLEQAGIWDEI+ +LK YELPDEFEG DEWI++GT+FR L EP
Sbjct: 421 DAFKLQKDPKDFQANIKRLEQAGIWDEIVEMLKRYELPDEFEGQDEWIQIGTRFRCLYEP 480

Query: 481 LDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILWAMVEELQIQT 540
           LDIANYYRHSKNDDTGPY+ +GRP RYRFTQRWLEH++K+ E SE S LWA VEEL+IQT
Sbjct: 481 LDIANYYRHSKNDDTGPYMIKGRPKRYRFTQRWLEHNQKMTEPSEVSTLWAEVEELRIQT 540

Query: 541 RTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRLQSCIKDDIER 600
           RT++YAE S EI+ELEKKMKRW+NEIE+DMLL+KSTF EWWK LPE HR QSCI+DDIER
Sbjct: 541 RTKLYAECSREILELEKKMKRWVNEIENDMLLKKSTFTEWWKTLPEHHRSQSCIRDDIER 600

Query: 601 MKNREDAIN 610
           M N EDA N
Sbjct: 601 MANTEDATN 605

BLAST of Tan0018253 vs. ExPASy TrEMBL
Match: A0A6J1E1J9 (protein EDS1L-like OS=Cucurbita moschata OX=3662 GN=LOC111429993 PE=4 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 1.8e-281
Identity = 489/609 (80.30%), Postives = 552/609 (90.64%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLENTRDFSIISFAGSWSVESWFSG 60
           MVH+RLE+ATGLK ELIG+AWSAA+KAHK+++KPFLL+ TRDFSIISFAGSWS E+WFS 
Sbjct: 34  MVHSRLEDATGLKEELIGNAWSAAVKAHKHADKPFLLDKTRDFSIISFAGSWSPEAWFSA 93

Query: 61  SDSSFGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPVVFTGH 120
           SDSSFGETKI+ QLFPS+RSIG+D+YA+VNSAF +RFE ILGKL K+VL+ NKPVVFTGH
Sbjct: 94  SDSSFGETKIDRQLFPSVRSIGLDEYAEVNSAFFRRFEAILGKL-KDVLKANKPVVFTGH 153

Query: 121 SAGGPIAILATIWLLEEQRKNSNKDINSTPPKCITFGSPLVGNFILSHALKREKWSIHFV 180
           SAGGP+AILATIWLLE+QR   N + N TPPKCITFGSPLVGNFI SHALKREKWSIHFV
Sbjct: 154 SAGGPVAILATIWLLEQQR---NSNTNFTPPKCITFGSPLVGNFIFSHALKREKWSIHFV 213

Query: 181 HFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNASTV 240
           HFV RYDIVPRIHLAPLPSLQP  LQTIL SLNSRSLGS+S+GNVAT FFMTV+ NAS V
Sbjct: 214 HFVTRYDIVPRIHLAPLPSLQP-HLQTILTSLNSRSLGSASNGNVATEFFMTVMRNASAV 273

Query: 241 ANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQILFYSC 300
           A+NAAC+LMGNT+LLLD LKSF+KLSPYSPFGTYIF ++SEKVVVVTNPDAVLQILF++C
Sbjct: 274 ASNAACHLMGNTNLLLDTLKSFVKLSPYSPFGTYIFFTDSEKVVVVTNPDAVLQILFFAC 333

Query: 301 QLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPITEALN 360
           QL+SE EC  IAHQSLK HWEYESKM+QNLELL+AIRLDEL KLPLSLTGR+TPIT+ALN
Sbjct: 334 QLSSESECDNIAHQSLKAHWEYESKMRQNLELLHAIRLDELDKLPLSLTGRNTPITQALN 393

Query: 361 QLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDGLGYY 420
           +LGLSS RALLNLRAAG++EEQK+KNQERM VKK+ IE+ LN L+ENYR VCKVDGLGYY
Sbjct: 394 ELGLSS-RALLNLRAAGSYEEQKIKNQERMSVKKQDIEKLLNRLEENYRDVCKVDGLGYY 453

Query: 421 DAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRRLVEP 480
           DAFKLQK+ KDF+ANIKRLEQAGIWDEI+ +LK YELPDEFEG DEWI++GT+FR L EP
Sbjct: 454 DAFKLQKDPKDFQANIKRLEQAGIWDEIVEMLKRYELPDEFEGQDEWIQIGTRFRCLYEP 513

Query: 481 LDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILWAMVEELQIQT 540
           LDIANYYRHSKNDDTGPY+ +GRP RYRFTQRWLEH++K+ E SE S LWA VEEL+IQT
Sbjct: 514 LDIANYYRHSKNDDTGPYMIKGRPKRYRFTQRWLEHNQKMTEPSEVSTLWAEVEELRIQT 573

Query: 541 RTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRLQSCIKDDIER 600
           RT++YAE S EI+ELEKKMKRW+NEIE+DMLL+KSTFMEWWK LPE HR QSCI+DDIER
Sbjct: 574 RTKLYAECSREILELEKKMKRWLNEIENDMLLKKSTFMEWWKTLPEHHRSQSCIRDDIER 633

Query: 601 MKNREDAIN 610
           M N EDA N
Sbjct: 634 MANTEDATN 636

BLAST of Tan0018253 vs. ExPASy TrEMBL
Match: A0A5D3BFM2 (Protein EDS1L-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002100 PE=4 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 7.3e-275
Identity = 483/609 (79.31%), Postives = 537/609 (88.18%), Query Frame = 0

Query: 1   MVHARLEEATGLKAELIGSAWSAAIKAHKYSEKPFLLENTRDFSIISFAGSWSVESWFSG 60
           M H +LE A  LK +LI SAWSAAIKAHK+ EKPFLL+ TRD SIISFAGS S E+WFS 
Sbjct: 1   MAHLKLEGAINLKEDLIASAWSAAIKAHKHPEKPFLLDKTRDSSIISFAGSSSPEAWFSA 60

Query: 61  SDSSFGETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRVNKPVVFTGH 120
           SDSSFGETKI+TQLFPS+RSIGVDDYA VNSAFL+RF+GI GKL KEVLRVNK VVFTGH
Sbjct: 61  SDSSFGETKIDTQLFPSVRSIGVDDYAVVNSAFLRRFQGIFGKL-KEVLRVNKSVVFTGH 120

Query: 121 SAGGPIAILATIWLLEEQRKNSNKDINSTPPKCITFGSPLVGNFILSHALKREKWSIHFV 180
           SAGGPIAILATIWLLE+QR   +    +  P CITFGSPLVGNFI SHALKREKWS +FV
Sbjct: 121 SAGGPIAILATIWLLEQQRNPDSNPYTNFTPTCITFGSPLVGNFIFSHALKREKWSTNFV 180

Query: 181 HFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNASTV 240
           HFV RYDIVPRIHLAPLPSLQP QLQTILN LNSRSLGS+ + NVAT FFMTV+ NAS V
Sbjct: 181 HFVTRYDIVPRIHLAPLPSLQP-QLQTILNCLNSRSLGSTMNANVATEFFMTVMRNASAV 240

Query: 241 ANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQILFYSC 300
            +NAAC LMGNT+LLLD LKSF+KLSPYSPFGTYIF +ESEKVVVV+NPDAVLQILFY+C
Sbjct: 241 VSNAACRLMGNTNLLLDTLKSFVKLSPYSPFGTYIFFTESEKVVVVSNPDAVLQILFYAC 300

Query: 301 QLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPITEALN 360
           QL+SE EC  IA QSLK HW YESKMQQNLE L+AIRLDEL KLPLSLTGR+TPITEAL+
Sbjct: 301 QLSSESECDYIAQQSLKAHWGYESKMQQNLEQLHAIRLDELAKLPLSLTGRNTPITEALD 360

Query: 361 QLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDGLGYY 420
           +LGL STRALLNLRAAGA+EEQK +N+ERME+KK+ IE+ LNWL+ENYRAVCKVDG GYY
Sbjct: 361 ELGL-STRALLNLRAAGAYEEQKTRNKERMELKKQYIEDRLNWLEENYRAVCKVDGFGYY 420

Query: 421 DAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRRLVEP 480
           DAFKLQK+ KDF+ANIKRLEQAGIWDEI+ +LK YELP+EFEG+DEWI+LGT+FRRLVEP
Sbjct: 421 DAFKLQKDPKDFQANIKRLEQAGIWDEIVEMLKRYELPEEFEGDDEWIKLGTRFRRLVEP 480

Query: 481 LDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDRKLREYSEESILWAMVEELQIQT 540
           LDIANYYRHSKNDDTGPYL +GRP RYRFTQRWLEH++K+ E SEES LWA VEE++I+T
Sbjct: 481 LDIANYYRHSKNDDTGPYLIKGRPKRYRFTQRWLEHNKKMSEPSEESTLWAKVEEIRIET 540

Query: 541 RTRMYAEFSGEIIELEKKMKRWINEIEDDMLLEKSTFMEWWKALPEQHRLQSCIKDDIER 600
           +T+MYAE S EIIELEKKMKRW+NEIEDDMLL+KSTFMEWWK LPE HR QSCIKDDIER
Sbjct: 541 KTKMYAECSREIIELEKKMKRWVNEIEDDMLLKKSTFMEWWKTLPEHHRSQSCIKDDIER 600

Query: 601 MKNREDAIN 610
           M N  D+ N
Sbjct: 601 MVNDNDSTN 606

BLAST of Tan0018253 vs. TAIR 10
Match: AT3G48090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 413.3 bits (1061), Expect = 3.4e-115
Identity = 254/635 (40.00%), Postives = 369/635 (58.11%), Query Frame = 0

Query: 7   EEATGLKAELIGSAWSAAIKAHKYSEKPFLLENTRDFSIISFAGSWSVESWFS-GSDSSF 66
           E  TG+  +LI  +WSA+ +A  Y  + +  E      I +F  S+S + +F   + SSF
Sbjct: 4   EALTGINGDLITRSWSASKQA--YLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSF 63

Query: 67  GETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILG------KLIKEVLRVNKPVVFTG 126
           GE K+N   FP +R IG  D A VN AFL+  E I+         ++  +R  K +VFTG
Sbjct: 64  GEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFTG 123

Query: 127 HSAGGPIAILATIWLLEEQ-RKNSNKDINSTPPKCITFGSPLVGNFILSHALKREKWSIH 186
           HS+GG  AILAT+W LE+   +N N  +    P+C+TFG+PLVG+ I SHAL REKWS  
Sbjct: 124 HSSGGATAILATVWYLEKYFIRNPNVYLE---PRCVTFGAPLVGDSIFSHALGREKWSRF 183

Query: 187 FVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNAS 246
           FV+FV R+DIVPRI LA   S++   L  +L  L+ R      S    T F+  V+ + S
Sbjct: 184 FVNFVSRFDIVPRIMLARKASVEET-LPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTS 243

Query: 247 TVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQILFY 306
           TVAN A C L G+    L+ L SF++LSPY P GT++F +E +++V V N DA+LQ+LFY
Sbjct: 244 TVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTE-KRLVAVNNSDAILQMLFY 303

Query: 307 SCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPITEA 366
           + Q + E+E   I  +S++DH  YE  +Q   +           KL   L G ++ I   
Sbjct: 304 TSQASDEQEWSLIPFRSIRDHHSYEELVQSMGK-----------KLFNHLDGENS-IEST 363

Query: 367 LNQLGLSSTRALLNLRAAGAFEEQKMKNQERM--EVKKKSIEEGLNWLQENYRAVCKVDG 426
           LN LG+ STR    ++AA   E+++++NQ+++   ++++   + L W+++ Y+  C+   
Sbjct: 364 LNDLGV-STRGRQYVQAALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHK 423

Query: 427 LGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRR 486
            GYYD+FK+   + DFKAN+KR E AG++DE+LG++K  +LPDEFEG+ +WI+L T++RR
Sbjct: 424 NGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRR 483

Query: 487 LVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEH-------------------- 546
           LVEPLDIANY+RH KN+DTGPY+K+GRP RY + QR  EH                    
Sbjct: 484 LVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNG 543

Query: 547 ---DRKLREYSEE---------SILWAMVEELQIQTRTRMYAEFSGEIIELEKKMKRWI- 597
                +L E  E          S  WA VEEL    + + Y E    +  LE  +  WI 
Sbjct: 544 LNLGLQLEEIQETLKNSGSECGSCFWAEVEEL----KGKPYEEVEVRVKTLEGMLGEWIT 603

BLAST of Tan0018253 vs. TAIR 10
Match: AT3G48080.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 406.8 bits (1044), Expect = 3.2e-113
Identity = 253/629 (40.22%), Postives = 361/629 (57.39%), Query Frame = 0

Query: 7   EEATGLKAELIGSAWSAAIKAHKYSEKPFLLENTRDFSIISFAGSWSVESWFSGSDSS-F 66
           E  TG+  +L+  +W A+  A++   + +L E        +F  S+S E  F+  ++S F
Sbjct: 4   EALTGVNGDLVTISWMASKGANQ--TEHYLKEEVGGTVFFAFRASFSSEDLFATENTSPF 63

Query: 67  GETKINTQLFPSIRSIGVDDYAQVNSAFLQRFEGILG------KLIKEVLRVNKPVVFTG 126
           GE K+    FP +RSIG D    VN AFL+  E ++G        ++  +   + VVFTG
Sbjct: 64  GEIKMKRNQFPCMRSIGNDVDTTVNEAFLKSLEVLIGPRTSFHASVQSAVDRKQQVVFTG 123

Query: 127 HSAGGPIAILATIWLLEEQRKNSNKDINSTP-PKCITFGSPLVGNFILSHALKREKWSIH 186
           HS GG  AILAT+W LE       +D  + P P+C+TFG+PLVG++I  HAL RE WS  
Sbjct: 124 HSFGGATAILATVWYLETY---FIRDAYAAPEPRCVTFGAPLVGDYIFKHALGRENWSRF 183

Query: 187 FVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNAS 246
           FV+FV R+DIVPRI LA   +++   L  +L  L+S       S  V T F+  V+ +  
Sbjct: 184 FVNFVTRFDIVPRIMLARKTTIEQT-LSYVLGKLDSTRAPIHESDQVITEFYTRVMRDTY 243

Query: 247 TVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVLQILFY 306
           TVA+ A C L+GN    L+ L SF +LSPY P GT++F S  +++VVV N DA+LQ+LFY
Sbjct: 244 TVASKAVCQLIGNGEAFLETLSSFYELSPYRPVGTFVF-STQKRLVVVNNSDAILQMLFY 303

Query: 307 SCQLNSERECGQIAHQSLKDHWEYESKMQQ-NLELLNAIRLDELVKLPLSLTGRSTPITE 366
           +CQ N E+E   I   S++DH  YE  +Q   ++LLN + L      PL L G ++ I  
Sbjct: 304 TCQSNDEQELSVIPFLSIRDHHGYEELVQSIGIKLLNHLDLHN----PL-LDGENS-IGS 363

Query: 367 ALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDGL 426
           AL+ LG+ STRA   + AA   E+Q+++NQ+++E K+  I E L W+ E Y+  C+    
Sbjct: 364 ALDDLGM-STRARQCIHAALEAEKQRVENQKKIETKRDQIVERLTWIVEVYKPKCQAHKN 423

Query: 427 GYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRRL 486
           GYYD+FK    + DFKAN+KR+E AGI+DE+LG++K  +LPD FEG+  WI L T++RRL
Sbjct: 424 GYYDSFKDSNEENDFKANVKRVELAGIFDEVLGLVKKGQLPDGFEGSRGWINLATQYRRL 483

Query: 487 VEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHD-------------------- 546
           +EPLDI+NY+   KN+DTGPY+  GRP+RY++ QR  EHD                    
Sbjct: 484 IEPLDISNYHGQLKNEDTGPYMLHGRPSRYKYAQRGYEHDILKPTGMIAKDVFWSKVNGL 543

Query: 547 ---------RKLREYSEE--SILWAMVEELQIQTRTRMYAEFSGEIIELEKKMKRWINEI 593
                      L+    E  S  WA VEEL    + + Y E       LE  ++ WI + 
Sbjct: 544 NLGLQQDIQEILKNSGSECGSCFWAEVEEL----KGKPYEEVQVRFKTLEGLLEGWIKDG 603

BLAST of Tan0018253 vs. TAIR 10
Match: AT3G48090.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 8.1e-101
Identity = 217/526 (41.25%), Postives = 314/526 (59.70%), Query Frame = 0

Query: 109 LRVNKPVVFTGHSAGGPIAILATIWLLEEQ-RKNSNKDINSTPPKCITFGSPLVGNFILS 168
           +R  K +VFTGHS+GG  AILAT+W LE+   +N N  +    P+C+TFG+PLVG+ I S
Sbjct: 3   VRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLE---PRCVTFGAPLVGDSIFS 62

Query: 169 HALKREKWSIHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVAT 228
           HAL REKWS  FV+FV R+DIVPRI LA   S++   L  +L  L+ R      S    T
Sbjct: 63  HALGREKWSRFFVNFVSRFDIVPRIMLARKASVEET-LPHVLAQLDPRKSSVQESEQRIT 122

Query: 229 AFFMTVLSNASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVT 288
            F+  V+ + STVAN A C L G+    L+ L SF++LSPY P GT++F +E +++V V 
Sbjct: 123 EFYTRVMRDTSTVANQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTE-KRLVAVN 182

Query: 289 NPDAVLQILFYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLS 348
           N DA+LQ+LFY+ Q + E+E   I  +S++DH  YE  +Q   +           KL   
Sbjct: 183 NSDAILQMLFYTSQASDEQEWSLIPFRSIRDHHSYEELVQSMGK-----------KLFNH 242

Query: 349 LTGRSTPITEALNQLGLSSTRALLNLRAAGAFEEQKMKNQERM--EVKKKSIEEGLNWLQ 408
           L G ++ I   LN LG+ STR    ++AA   E+++++NQ+++   ++++   + L W++
Sbjct: 243 LDGENS-IESTLNDLGV-STRGRQYVQAALEEEKKRVENQKKIIQVIEQERFLKKLAWIE 302

Query: 409 ENYRAVCKVDGLGYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGND 468
           + Y+  C+    GYYD+FK+   + DFKAN+KR E AG++DE+LG++K  +LPDEFEG+ 
Sbjct: 303 DEYKPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLMKKCQLPDEFEGDI 362

Query: 469 EWIRLGTKFRRLVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEH--------- 528
           +WI+L T++RRLVEPLDIANY+RH KN+DTGPY+K+GRP RY + QR  EH         
Sbjct: 363 DWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMI 422

Query: 529 --------------DRKLREYSEE---------SILWAMVEELQIQTRTRMYAEFSGEII 588
                           +L E  E          S  WA VEEL    + + Y E    + 
Sbjct: 423 AEDVFWNKVNGLNLGLQLEEIQETLKNSGSECGSCFWAEVEEL----KGKPYEEVEVRVK 482

Query: 589 ELEKKMKRWI--NEIED-DMLLEKSTFMEWWKALPEQHRLQSCIKD 597
            LE  +  WI   E++D ++ LE STF +WW  LP+ H+  S ++D
Sbjct: 483 TLEGMLGEWITDGEVDDKEIFLEGSTFRKWWITLPKNHKSHSPLRD 506

BLAST of Tan0018253 vs. TAIR 10
Match: AT3G52430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 119.8 bits (299), Expect = 7.7e-27
Identity = 123/486 (25.31%), Postives = 189/486 (38.89%), Query Frame = 0

Query: 74  LFPSIRSIGVDDYAQVNSAFLQRFEGILGKLIKEVLRV----NKPVVFTGHSAGGPIAIL 133
           LFP + S   +    V++A L+ F   L   IKE L +     K VV TGHS GG +A  
Sbjct: 72  LFPGLSS--DEPLPMVDAAILKLF---LQLKIKEGLELELLGKKLVVITGHSTGGALAAF 131

Query: 134 ATIWLLEEQRKNSNKDINSTPPK----CITFGSPLVGNFILSHALKREKWSIHFVHFVMR 193
             +WLL +          S+PP     CITFGSPL+GN  LS ++ R + + +F H V  
Sbjct: 132 TALWLLSQ----------SSPPSFRVFCITFGSPLLGNQSLSTSISRSRLAHNFCHVVSI 191

Query: 194 YDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTVLSNASTVANNAA 253
           +D+VPR                              S N                     
Sbjct: 192 HDLVPR------------------------------SSN--------------------- 251

Query: 254 CNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVV--VTNPDAVLQILFYSCQLN 313
                                 + PFGTY+FCS+   V +    +   +  IL  +   N
Sbjct: 252 --------------------EQFWPFGTYLFCSDKGGVCLDNAGSVRLMFNILNTTATQN 311

Query: 314 SERE------CGQIAHQSLKDHWEYESKMQQNLELLNAIRLDELVKLPLSLTGRSTPITE 373
           +E           ++H  LK        +  N          E   L  S    S  + +
Sbjct: 312 TEEHQRYGHYVFTLSHMFLKSRSFLGGSIPDNSYQAGVALAVE--ALGFSNDDTSGVLVK 371

Query: 374 ALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAVCKVDGL 433
              +      RA + LR+A    E       R+E++         W ++  R     + L
Sbjct: 372 ECIETATRIVRAPI-LRSAELANELASVLPARLEIQ---------WYKD--RCDASEEQL 431

Query: 434 GYYDAFKLQKNDKDFKANIKRLEQAGIWDEILGILKMYELPDEFEGNDEWIRLGTKFRRL 493
           GYYD FK     +DFK N+ R+  A  WD ++ +++  ELP +F    +WI     ++ L
Sbjct: 432 GYYDFFKRYSLKRDFKVNMSRIRLAKFWDTVIKMVETNELPFDFHLGKKWIYASQFYQLL 457

Query: 494 VEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRWLEHDR--------KLREYSEESIL 536
            EPLDIAN+Y++      G YL+  RP RY    +W +  +        +    ++++  
Sbjct: 492 AEPLDIANFYKNRDIKTGGHYLEGNRPKRYEVIDKWQKGVKVPEECVRSRYASTTQDTCF 457

BLAST of Tan0018253 vs. TAIR 10
Match: AT5G14930.2 (senescence-associated gene 101 )

HSP 1 Score: 107.8 bits (268), Expect = 3.0e-23
Identity = 143/624 (22.92%), Postives = 231/624 (37.02%), Query Frame = 0

Query: 16  LIGSAWSAAIKAH-----------------KYSEKPFLLENTRDFSIISFAGSWSVESWF 75
           L+ S+WS  ++ H                 K S   F +     F+++ F+      S  
Sbjct: 21  LLHSSWSKILEIHNPPYSNHDPGLQVSKKKKDSGLEFQIHREEKFTLVVFSAPPICRS-- 80

Query: 76  SGSDSSFGETKINTQLFPSIRSIGVDDYAQVNSAF-----LQRFEGILGKLIKEVLRVNK 135
           S SDS+    K     FP + S     ++    AF            L   + + L+  K
Sbjct: 81  SSSDSTLLHVKDKENPFPFLCSENNPSFSLHTPAFNLFTSASTSLTYLKSELLQTLKSEK 140

Query: 136 PVVFTGHSAGGPIAILATIWLLEEQRKNSNKDINSTPPKCITFGSPLVGNFILSHALKRE 195
           PV+ TG + GG +A L T+WLLE       +      P CITFGSPL+G+  L   L+  
Sbjct: 141 PVIITGAALGGSVASLYTLWLLETIEPTLKR------PLCITFGSPLIGDASLQQILENS 200

Query: 196 KWSIHFVHFVMRYDIVPRIHLAPLPSLQPVQLQTILNSLNSRSLGSSSSGNVATAFFMTV 255
             +  F+H V                                                  
Sbjct: 201 VRNSCFLHVV-------------------------------------------------- 260

Query: 256 LSNASTVANNAACNLMGNTSLLLDNLKSFIKLSPYSPFGTYIFCSESEKVVVVTNPDAVL 315
                                   + ++ IK+  + PFGT++ C +S   V + +  AV 
Sbjct: 261 ------------------------SAQTRIKMDFFKPFGTFLICFDS-GCVCIEDHVAVT 320

Query: 316 QILFYSCQLNSERECGQIAHQSLKDHWEYESKMQQNLELLNAIRL--DELVKLPLSLTGR 375
           ++      LN   + G      L D+ +  +++ Q++  L   RL  ++++K        
Sbjct: 321 EL------LNGVHDSG------LVDYSQVLNRLDQSMLSLADSRLIPEDVIK-------- 380

Query: 376 STPITEALNQLGLSSTRALLNLRAAGAFEEQKMKNQERMEVKKKSIEEGLNWLQENYRAV 435
                      G+     + NLR      +   K    M++    IE    W    Y+  
Sbjct: 381 -----------GIEKRAEMKNLRF-----DMMFKKLNDMKISMAYIE----W----YKKK 440

Query: 436 CKVDGLGYYDAFKLQK--NDKDFKANIK---RLEQAGIWDEILGILKMYELPDEFEGNDE 495
           CK   +GYYD FK Q     K+F  NIK   + E    W  ++  ++     D       
Sbjct: 441 CKEVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASILKRR 500

Query: 496 WIRLGTKFRRLVEPLDIANYYRHSKNDDTGPYLKQGRPNRYRFTQRW-------LEHDR- 555
           ++  G  +RR++EPLDIA YY   + +    Y   GR + Y   ++W       +E +R 
Sbjct: 501 FLFSGNNYRRMIEPLDIAEYYLEGRKE----YRTTGRSHHYVMLEKWFGMESILIEKERC 508

Query: 556 KLREYSE----ESILWAMVEELQI----------------QTRTRMYAEFSGEIIELEKK 583
           K R+ S+    +S  WA VE+  I                +  TR   EF G + E+  K
Sbjct: 561 KKRDLSDLLTFDSCFWAEVEDSLIVINQLNTTVGMRDDVREVLTRKLVEFEGYVWEIITK 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XF231.8e-11640.09Protein EDS1L OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1[more]
Q9SU724.8e-11440.00Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1[more]
Q9SU714.5e-11240.22Protein EDS1B OS=Arabidopsis thaliana OX=3702 GN=EDS1B PE=1 SV=1[more]
Q9S7451.1e-2525.31Lipase-like PAD4 OS=Arabidopsis thaliana OX=3702 GN=PAD4 PE=1 SV=1[more]
Q4F8834.3e-2222.92Senescence-associated carboxylesterase 101 OS=Arabidopsis thaliana OX=3702 GN=SA... [more]
Match NameE-valueIdentityDescription
XP_023529901.11.3e-30284.39protein EDS1L-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023529896.11.3e-30284.39protein EDS1L-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529897.1 prote... [more]
XP_022972365.11.3e-30284.38protein EDS1L-like [Cucurbita maxima] >XP_022972376.1 protein EDS1L-like [Cucurb... [more]
KAG7021481.11.7e-30284.72Protein EDS1L, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6587497.11.4e-30184.74Protein EDS1L, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1I8E26.3e-30384.38protein EDS1L-like OS=Cucurbita maxima OX=3661 GN=LOC111470937 PE=4 SV=1[more]
A0A6J1F4K94.5e-30184.33protein EDS1L-like OS=Cucurbita moschata OX=3662 GN=LOC111440567 PE=4 SV=1[more]
A0A6J1JLB18.6e-28480.46protein EDS1L-like OS=Cucurbita maxima OX=3661 GN=LOC111485621 PE=4 SV=1[more]
A0A6J1E1J91.8e-28180.30protein EDS1L-like OS=Cucurbita moschata OX=3662 GN=LOC111429993 PE=4 SV=1[more]
A0A5D3BFM27.3e-27579.31Protein EDS1L-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G0... [more]
Match NameE-valueIdentityDescription
AT3G48090.13.4e-11540.00alpha/beta-Hydrolases superfamily protein [more]
AT3G48080.13.2e-11340.22alpha/beta-Hydrolases superfamily protein [more]
AT3G48090.28.1e-10141.25alpha/beta-Hydrolases superfamily protein [more]
AT3G52430.17.7e-2725.31alpha/beta-Hydrolases superfamily protein [more]
AT5G14930.23.0e-2322.92senescence-associated gene 101 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 545..565
NoneNo IPR availablePANTHERPTHR47090:SF2PROTEIN EDS1-RELATEDcoord: 1..598
IPR041266EDS1, EP domainPFAMPF18117EDS1_EPcoord: 406..595
e-value: 1.6E-50
score: 171.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 26..332
e-value: 2.7E-24
score: 87.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 40..196
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 45..193
e-value: 1.1E-18
score: 67.5
IPR044214EDS1-likePANTHERPTHR47090PROTEIN EDS1-RELATEDcoord: 1..598

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0018253.1Tan0018253.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005634 nucleus