Tan0018221 (gene) Snake gourd v1

Overview
NameTan0018221
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptioncalmodulin-like
LocationLG01: 16370109 .. 16370381 (+)
RNA-Seq ExpressionTan0018221
SyntenyTan0018221
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCGCAAAAGCAAAAGGGAACAGCAGAGGCCTAAGATATCAAGACAGCAACTAAGTTTAATCTTCAAAAACCATGACCGCGACGGCGACGGCCGCCTCAGCGCCACCGACCTTACTGAAGCCTTCCACTATATCGGCGCCCTTCTGCCGTCTTACCGAGCTTGCCACGCTCTTCTTCACACCGATTCCGATCGAGATGGATTCATCTGCGAGAAGGATTTCGAATTGCTCGTCAATTATGCCGAGAAACGCAAATACACAGTCATTTGA

mRNA sequence

ATGTCTCGCAAAAGCAAAAGGGAACAGCAGAGGCCTAAGATATCAAGACAGCAACTAAGTTTAATCTTCAAAAACCATGACCGCGACGGCGACGGCCGCCTCAGCGCCACCGACCTTACTGAAGCCTTCCACTATATCGGCGCCCTTCTGCCGTCTTACCGAGCTTGCCACGCTCTTCTTCACACCGATTCCGATCGAGATGGATTCATCTGCGAGAAGGATTTCGAATTGCTCGTCAATTATGCCGAGAAACGCAAATACACAGTCATTTGA

Coding sequence (CDS)

ATGTCTCGCAAAAGCAAAAGGGAACAGCAGAGGCCTAAGATATCAAGACAGCAACTAAGTTTAATCTTCAAAAACCATGACCGCGACGGCGACGGCCGCCTCAGCGCCACCGACCTTACTGAAGCCTTCCACTATATCGGCGCCCTTCTGCCGTCTTACCGAGCTTGCCACGCTCTTCTTCACACCGATTCCGATCGAGATGGATTCATCTGCGAGAAGGATTTCGAATTGCTCGTCAATTATGCCGAGAAACGCAAATACACAGTCATTTGA

Protein sequence

MSRKSKREQQRPKISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEKDFELLVNYAEKRKYTVI
Homology
BLAST of Tan0018221 vs. ExPASy Swiss-Prot
Match: Q39419 (Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.0e-04
Identity = 22/54 (40.74%), Postives = 33/54 (61.11%), Query Frame = 0

Query: 22 IFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEKDF 76
          IFK  D +GDG++SA +L EA   +G++ P     H +   D+D DGFI  ++F
Sbjct: 15 IFKRFDANGDGKISAAELGEALKTLGSITPD-EVKHMMAEIDTDGDGFISFQEF 67

BLAST of Tan0018221 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 45.1 bits (105), Expect = 5.0e-04
Identity = 21/61 (34.43%), Postives = 35/61 (57.38%), Query Frame = 0

Query: 15  SRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEKD 74
           S+++L  +F + D DGDG++SA +L   F  +G  +    A  A+   D+D DG +  +D
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

Query: 75  F 76
           F
Sbjct: 121 F 121

BLAST of Tan0018221 vs. ExPASy Swiss-Prot
Match: O81701 (Polcalcin Aln g 4 OS=Alnus glutinosa OX=3517 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.0e-04
Identity = 21/54 (38.89%), Postives = 34/54 (62.96%), Query Frame = 0

Query: 22 IFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEKDF 76
          IFK  D +GDG++SA++L +A   +G++ P     H +   D+D DGFI  ++F
Sbjct: 15 IFKCFDANGDGKISASELGDALKTLGSVTPD-EVKHMMAEIDTDGDGFISFQEF 67

BLAST of Tan0018221 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 6.6e-04
Identity = 20/58 (34.48%), Postives = 32/58 (55.17%), Query Frame = 0

Query: 18 QLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEKDF 76
          +L  +F+ +D +GDG++SA +L      +GA L        +   DSDRDGF+   +F
Sbjct: 5  ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

BLAST of Tan0018221 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 6.6e-04
Identity = 24/65 (36.92%), Postives = 33/65 (50.77%), Query Frame = 0

Query: 20 SLIFKNHDRDGDGRLSATDLTEAFH-YIGALLPSYRACHALLHTDSDRDGFICEKDFELL 79
          S +F   D DGDGR+SA +L       +G  +    A   +   D+D DG +CE +F  L
Sbjct: 13 SALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRL 72

Query: 80 VNYAE 84
          V  AE
Sbjct: 73 VQAAE 77

BLAST of Tan0018221 vs. NCBI nr
Match: KAG6600743.1 (Calcium-binding protein CML38, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031384.1 Calcium-binding protein CML38, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 148.7 bits (374), Expect = 2.5e-32
Identity = 71/90 (78.89%), Postives = 82/90 (91.11%), Query Frame = 0

Query: 1  MSRKSKREQQRPKISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALL 60
          M RK+K   QRPKISRQQL +IF+NHDRDGDGRLS+ +LT+AFHYIGALLPS+RACHALL
Sbjct: 1  MPRKTKMYHQRPKISRQQLRVIFRNHDRDGDGRLSSAELTKAFHYIGALLPSFRACHALL 60

Query: 61 HTDSDRDGFICEKDFELLVNYAEKRKYTVI 91
          +TDSDRDGFI EKDFE L++YAEKR+YTVI
Sbjct: 61 YTDSDRDGFIDEKDFEQLIDYAEKRQYTVI 90

BLAST of Tan0018221 vs. NCBI nr
Match: KAG7024248.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 147.1 bits (370), Expect = 7.2e-32
Identity = 73/90 (81.11%), Postives = 79/90 (87.78%), Query Frame = 0

Query: 1  MSRKSKREQQRPKISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALL 60
          M+RKSK  Q RPK+SR+QLS+IF+NHD DGDGRLS T+LT AF YIGA LPSYRA HALL
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HTDSDRDGFICEKDFELLVNYAEKRKYTVI 91
          HTDSD DGFI EKDFELLVNYAEKRKYTVI
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYTVI 90

BLAST of Tan0018221 vs. NCBI nr
Match: KAG6591370.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 144.1 bits (362), Expect = 6.1e-31
Identity = 71/88 (80.68%), Postives = 77/88 (87.50%), Query Frame = 0

Query: 1  MSRKSKREQQRPKISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALL 60
          M+RKSK  Q RPK+SR+QLS+IF+NHD DGDGRLS T+LT AF YIGA LPSYRA HALL
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HTDSDRDGFICEKDFELLVNYAEKRKYT 89
          HTDSD DGFI EKDFELLVNYAEKRKYT
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYT 88

BLAST of Tan0018221 vs. NCBI nr
Match: KAG6608481.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037808.1 putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 141.0 bits (354), Expect = 5.1e-30
Identity = 68/90 (75.56%), Postives = 78/90 (86.67%), Query Frame = 0

Query: 1  MSRKSKREQQRPKISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALL 60
          M+RK+K  Q RPK+SR+QLS+IF+NHD DGDGRLSAT+LTEAF YIGA LPSYRA HAL 
Sbjct: 1  MARKNKMSQPRPKLSREQLSVIFRNHDGDGDGRLSATELTEAFRYIGAFLPSYRAHHALR 60

Query: 61 HTDSDRDGFICEKDFELLVNYAEKRKYTVI 91
          +TDSDRDGFI EKDFE LVNY EKR YT++
Sbjct: 61 YTDSDRDGFIGEKDFESLVNYTEKRHYTIV 90

BLAST of Tan0018221 vs. NCBI nr
Match: KAG6600744.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 141.0 bits (354), Expect = 5.1e-30
Identity = 67/90 (74.44%), Postives = 80/90 (88.89%), Query Frame = 0

Query: 1  MSRKSKREQQRPKISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALL 60
          M RK+K    RP+ISR+QL LIF+NHD +GDGRLS+ +LT+AFHYIGALLPS+RACHALL
Sbjct: 1  MPRKTKTYHPRPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALL 60

Query: 61 HTDSDRDGFICEKDFELLVNYAEKRKYTVI 91
          +TDSDRDGFI EKDFE L++YAEKR+YTVI
Sbjct: 61 YTDSDRDGFIDEKDFEQLIDYAEKRQYTVI 90

BLAST of Tan0018221 vs. ExPASy TrEMBL
Match: A0A0A0KZR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.8e-28
Identity = 67/90 (74.44%), Postives = 75/90 (83.33%), Query Frame = 0

Query: 1  MSRKSKREQQRPKISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALL 60
          MSRKSK    R K+SR+QL LIF+NHD D DGRLS T+LTEAF YIGA LP+YRA HALL
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HTDSDRDGFICEKDFELLVNYAEKRKYTVI 91
          H DSDRDGFI EKDFE LVNYAE+R+YT+I
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

BLAST of Tan0018221 vs. ExPASy TrEMBL
Match: A0A5A7VDB9 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003480 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 4.0e-28
Identity = 66/90 (73.33%), Postives = 74/90 (82.22%), Query Frame = 0

Query: 1  MSRKSKREQQRPKISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALL 60
          MSR+SK    R ++SR+QL LIF+NHD D DGRLS T+LTEAF YIGA LP YRA HAL 
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HTDSDRDGFICEKDFELLVNYAEKRKYTVI 91
          H DSDRDGFI EKDFELLVNYAE+R+YTVI
Sbjct: 61 HADSDRDGFISEKDFELLVNYAEQRQYTVI 90

BLAST of Tan0018221 vs. ExPASy TrEMBL
Match: A5AWP8 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.8e-12
Identity = 38/76 (50.00%), Postives = 52/76 (68.42%), Query Frame = 0

Query: 14 ISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEK 73
          +  QQL  IFK HD +GDG+L+  +L +AF ++GAL+P +RA  AL H D+D DG I EK
Sbjct: 15 LPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTDGDGCISEK 74

Query: 74 DFELLVNYAEKRKYTV 90
          + + LV YA K  YT+
Sbjct: 75 EMKDLVEYAAKFGYTI 90

BLAST of Tan0018221 vs. ExPASy TrEMBL
Match: A0A5N6RQV2 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.0e-12
Identity = 38/75 (50.67%), Postives = 53/75 (70.67%), Query Frame = 0

Query: 15 SRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEKD 74
          +R QL  IF++HD DGDG+LS  ++  AF Y+G+   SYRA  ALLH DSD DG++ E++
Sbjct: 16 TRDQLMNIFRSHDLDGDGKLSRKEVKAAFGYLGSRWDSYRAVLALLHADSDHDGYVGEEE 75

Query: 75 FELLVNYAEKRKYTV 90
           + LV+YA K  YT+
Sbjct: 76 LKKLVDYALKCGYTI 90

BLAST of Tan0018221 vs. ExPASy TrEMBL
Match: A0A6J1KUW2 (calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.8e-12
Identity = 35/72 (48.61%), Postives = 52/72 (72.22%), Query Frame = 0

Query: 13  KISRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICE 72
           K+SR Q+  IF  HD DGDG LS ++LT+AF + G+++P Y+A + L + D+D DG I E
Sbjct: 84  KLSRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGLAYADADGDGLISE 143

Query: 73  KDFELLVNYAEK 85
           ++ E LV+YAE+
Sbjct: 144 EELEKLVDYAER 155

BLAST of Tan0018221 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 45.1 bits (105), Expect = 3.6e-05
Identity = 21/61 (34.43%), Postives = 35/61 (57.38%), Query Frame = 0

Query: 15  SRQQLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEKD 74
           S+++L  +F + D DGDG++SA +L   F  +G  +    A  A+   D+D DG +  +D
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

Query: 75  F 76
           F
Sbjct: 121 F 121

BLAST of Tan0018221 vs. TAIR 10
Match: AT3G51920.1 (calmodulin 9 )

HSP 1 Score: 41.6 bits (96), Expect = 3.9e-04
Identity = 20/62 (32.26%), Postives = 33/62 (53.23%), Query Frame = 0

Query: 18  QLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEKDFEL 77
           +L  +F+  DRDGDG +S  +L E    +G  + +  A H +   D D DGF+   +F  
Sbjct: 85  ELIEVFRVFDRDGDGLISQLELGEGMKDMGMKITAEEAEHMVREADLDGDGFLSFHEFSK 144

Query: 78  LV 80
           ++
Sbjct: 145 MM 146

BLAST of Tan0018221 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 41.6 bits (96), Expect = 3.9e-04
Identity = 16/58 (27.59%), Postives = 34/58 (58.62%), Query Frame = 0

Query: 18 QLSLIFKNHDRDGDGRLSATDLTEAFHYIGALLPSYRACHALLHTDSDRDGFICEKDF 76
          +L+ +F+  D+DGDG+++  +L E+F  +G ++P       +   D + DG +  ++F
Sbjct: 5  ELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEF 62

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q394191.0e-0440.74Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1[more]
Q8L3R25.0e-0434.43Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ... [more]
O817015.0e-0438.89Polcalcin Aln g 4 OS=Alnus glutinosa OX=3517 PE=1 SV=1[more]
Q0DJV66.6e-0434.48Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q8RYK06.6e-0436.92Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
KAG6600743.12.5e-3278.89Calcium-binding protein CML38, partial [Cucurbita argyrosperma subsp. sororia] >... [more]
KAG7024248.17.2e-3281.11Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6591370.16.1e-3180.68Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6608481.15.1e-3075.56Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037808.1... [more]
KAG6600744.15.1e-3074.44Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A0A0KZR61.8e-2874.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1[more]
A0A5A7VDB94.0e-2873.33Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A5AWP81.8e-1250.00Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1[more]
A0A5N6RQV24.0e-1250.67Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1[more]
A0A6J1KUW26.8e-1248.61calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G50770.13.6e-0534.43calmodulin-like 41 [more]
AT3G51920.13.9e-0432.26calmodulin 9 [more]
AT4G03290.13.9e-0427.59EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.238.10coord: 1..86
e-value: 2.2E-9
score: 39.4
NoneNo IPR availablePANTHERPTHR10891:SF911SUBFAMILY NOT NAMEDcoord: 13..84
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 13..84
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 63..75
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 27..39
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 14..49
score: 12.05861
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 6..80

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0018221.1Tan0018221.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding