Tan0017682 (gene) Snake gourd v1

BLAST of Tan0017682 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 5.5e-282
Identity = 497/732 (67.90%), Postives = 582/732 (79.51%), Query Frame = 0

Query: 7   MSCSLIFNLVFLSL-FSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAMLEEVV 66
           MS SLIF L F SL FS  LAS  DSDDDG+ IYIVYMG KL+DPDSAHLHHRAMLE+VV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 67  GSNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSF 126
           GS FAPESV++TYKRSFNGFAVKL EEEA+KIA+ EGVVSVF +E N+LHTTRSWDFL F
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 127 PLYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIG 186
           PL VPR ++VESN +VGVLD+GIW + PSF D+G+ PPP KWKG C+ S N  CNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 187 ARAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIA 246
           AR+Y     + PGD+  P DTNGHGTHTAST AG LV++A++YGLGLGTARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 247 VYKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGIL 306
            YKVCW+DGCSD DILAA+DDAIADGVDI+S+S+GG  P+ YF+ +I+IG+FHA++ GIL
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 307 TSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTADDKKNL 366
           TSNSAGN GP   TT SL+PW+LSVAASTMDRKFVT VQ+GN    QG++INT D+ +  
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDN-QYY 360

Query: 367 SLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVM-PAKAFLSFGEAAGVLMQ 426
            LV G D+PN GF  S SR+C   SVNP L++GKI+VC+A   P + F S   AAGVLM 
Sbjct: 361 PLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMT 420

Query: 427 GYSSS-ARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRG 486
             +   A SYPLPSS+L+P D     RY+ S R P AT+FKSTTI +ASAP V+SFSSRG
Sbjct: 421 SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRG 480

Query: 487 PNSLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAY 546
           PN  T D++KPD+S PGVEILAAWP +AP  V    R  L+NIISGTSMSCPH+T IA Y
Sbjct: 481 PNRATKDVIKPDISGPGVEILAAWPSVAP--VGGIRRNTLFNIISGTSMSCPHITGIATY 540

Query: 547 VKTFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAKESDY 606
           VKT++PTWSPAAIKSALMTTASPM  + N +AEFAYGSG V+PLKA+ PGLVYDA ESDY
Sbjct: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600

Query: 607 VKFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFLRTV 666
           VKFLCG+GYNT  VRRITGD SACTS N GRVWDLNYPSF LSV+ SQ    NQ F RT+
Sbjct: 601 VKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQ--TFNQYFNRTL 660

Query: 667 TSVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISASLVWS 726
           TSV    ST+RA IS  QGLTI+V+P+VLSFNG+GD+KSFTLTV+G++    +SASLVWS
Sbjct: 661 TSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWS 720

Query: 727 NGLFKVRSPITV 736
           +G+  VRSPIT+
Sbjct: 721 DGVHYVRSPITI 727

BLAST of Tan0017682 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 5.7e-170
Identity = 343/743 (46.16%), Postives = 470/743 (63.26%), Query Frame = 0

Query: 17  FLSLFSGLLASGF-----DSDDDG-RKIYIVYMGS-KLKDPDSAHLHHRAMLEEVVGSNF 76
           F+ LFS LL         D DD G +++YIVY+GS   ++  +    H ++L+E+ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 77  APESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSFP--L 136
               ++ +YK+SFNGFA +L E E +++A  E VVSVFPS + +L TT SW+F+     +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 137 YVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGAR 196
              R   +ES+TI+GV+DSGI+ +  SFSDQG+ PPP KWKG C    N +CN K+IGAR
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 186

Query: 197 AYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAVY 256
            Y ++S       ++  D +GHGTHTAS  AG+ V  ++ YGLG GTARGGVP+ARIAVY
Sbjct: 187 DYTAKSKAN----QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVY 246

Query: 257 KVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGILTS 316
           KVC ++GC    +++AFDDAIADGVD++S+S+      P+    I+IGAFHAM  G+LT 
Sbjct: 247 KVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTV 306

Query: 317 NSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD-DKKNLS 376
           N+AGN+GP  ST TS APW+ SVAAS  +R F+  V LG+  I  G ++NT D +  N  
Sbjct: 307 NAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYP 366

Query: 377 LVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLMQGY 436
           LVYG            +R CE   ++ KLV+GKI++CD+          G    ++    
Sbjct: 367 LVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPE 426

Query: 437 SSSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRGPNS 496
              A     P S L+  D  ++  YM ST+ P AT+ KS  I +  AP V SFSSRGP+S
Sbjct: 427 PDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSS 486

Query: 497 LTSDILKPDLSAPGVEILAAW-PPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVK 556
           + SDILKPD++APGVEILAA+ P  +P     D+RRV Y+++SGTSM+CPHV  +AAYVK
Sbjct: 487 IVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVK 546

Query: 557 TFHPTWSPAAIKSALMTTASPMKEKIN--KEAEFAYGSGQVDPLKAIDPGLVYDAKESDY 616
           TFHP WSP+ I+SA+MTTA PM    +     EFAYGSG VDP+ AI+PGLVY+  ++D+
Sbjct: 547 TFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADH 606

Query: 617 VKFLCGEGYNTTMVRRITGDNSACT---SANIGRVWDLNYPSFALSVARSQISNINQNFL 676
           + FLCG  Y +  +R I+GDNS CT   S  + R  +LNYP+ +  V+ ++  NI   F 
Sbjct: 607 INFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTKPFNI--TFQ 666

Query: 677 RTVTSVVSGKSTFRATISGAQG--LTITVSPSVLSFNGIGDQKSFTLTVQGAM--TESRI 736
           RTVT+V   KST+ A +    G  L+I VSP VLS   + +++SF +TV      T+  +
Sbjct: 667 RTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPV 726

Query: 737 SASLVWSNGLFKVRSPITVYTRS 740
           SA+L+WS+G   VRSPI VY  S
Sbjct: 727 SANLIWSDGTHNVRSPIIVYAMS 741

BLAST of Tan0017682 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 1.3e-169
Identity = 329/709 (46.40%), Postives = 458/709 (64.60%), Query Frame = 0

Query: 38  IYIVYMGSKLKDPDSAHLHHRAMLEEVVGSNFAPESVIYTYKRSFNGFAVKLNEEEAQKI 97
           +YIVYMG+  +   S   HH ++L+++VG+  A   ++ +YKRSFNGFA  L++ E+QK+
Sbjct: 32  VYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKL 91

Query: 98  ATKEGVVSVFPSEENQLHTTRSWDFLSFPLYVPRVNEVESNTIVGVLDSGIWSDHPSFSD 157
              + VVSVFPS+ ++L TTRSWDF+ F     R +  ES+ IVGV+DSGIW +  SF D
Sbjct: 92  QNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDD 151

Query: 158 QGYDPPPAKWKGICQASTNISCNRKIIGARAYRSRSSLPPGDIRSPIDTNGHGTHTASTV 217
           +G+ PPP KWKG C+     +CN K+IGAR Y   +        S  D  GHGTHTAST 
Sbjct: 152 EGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTA 211

Query: 218 AGSLVNKASMYGLGLGTARGGVPSARIAVYKVCWSDGCSDADILAAFDDAIADGVDILSV 277
           AG+ V  AS YGL  GTARGGVPSARIA YKVC+ + C+D DILAAFDDAIADGVD++S+
Sbjct: 212 AGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISI 271

Query: 278 SLGGTRPQPYFLHSISIGAFHAMKHGILTSNSAGNSGPGRSTTTSLAPWILSVAASTMDR 337
           S+           S++IG+FHAM  GI+T+ SAGN+GP + +  +++PW+++VAAS  DR
Sbjct: 272 SISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDR 331

Query: 338 KFVTPVQLGNTNIHQGIAINTAD-DKKNLSLVYGGDVPNVGFRSSVSRYCEINSVNPKLV 397
           +F+  V LGN     GI++NT + +     +VYG +V       + + YC    V+ +LV
Sbjct: 332 QFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELV 391

Query: 398 RGKILVCDAVMPAKAFLSFGEAAGVLMQG--YSSSARSYPLPSSILNPTDGTNVKRYMTS 457
           +GKI++CD  +  +     G A GV++Q      SA   P P+S L   D  ++K Y+ S
Sbjct: 392 KGKIVLCDDFLGYREAYLAG-AIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIES 451

Query: 458 TRFPTATLFKSTTIPDASAPAVISFSSRGPNSLTSDILKPDLSAPGVEILAAWPPIA--- 517
              P A + ++  I D  AP V SFSSRGP+ +  ++LKPD+SAPG+EILAA+ P+A   
Sbjct: 452 AEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPS 511

Query: 518 PITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVKTFHPTWSPAAIKSALMTTASPMKEKI 577
               P+D R V Y+++SGTSM+CPHV  +AAYVK+FHP WSP+AIKSA+MTTA+PM  K 
Sbjct: 512 SFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKK 571

Query: 578 NKEAEFAYGSGQVDPLKAIDPGLVYDAKESDYVKFLCGEGYNTTMVRRITGDNSACTSAN 637
           N E EFAYGSGQ++P KA DPGLVY+ +  DY+K LC EG+++T +   +G N  C+   
Sbjct: 572 NPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT 631

Query: 638 IGRVWDLNYPSFALSVARSQISNINQNFLRTVTSVVSGKSTFRATISGAQ-GLTITVSPS 697
              V DLNYP+    V  S +   N  F RTVT+V    ST++A++   Q  L I++ P 
Sbjct: 632 --EVKDLNYPTMTTFV--SSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPE 691

Query: 698 VLSFNGIGDQKSFTLTVQGAMTE--SRISASLVWSNGLFKVRSPITVYT 738
           +L F  + ++KSF +T+ G   +  S +S+S+VWS+G   VRSPI  Y+
Sbjct: 692 ILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 726

BLAST of Tan0017682 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 4.8e-169
Identity = 341/734 (46.46%), Postives = 461/734 (62.81%), Query Frame = 0

Query: 18  LSLFSGLLASGFDS---DDDGRKIYIVYMGSKLKDPDSAHL-HHRAMLEEVVGSNFAPES 77
           LS    LL   F S   DD  +++YIVYMG+     D   + HH ++L++V G +   + 
Sbjct: 10  LSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQDR 69

Query: 78  VIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSFP--LYVPR 137
           ++  YKRSFNGFA +L E E + +A+ + VVSVFPS+   L TT SW+F+         R
Sbjct: 70  LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 129

Query: 138 VNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGARAYRS 197
              +ES+TI+GV+DSGI+ +  SFS +G+ PPP KWKG+C+  TN +CN K+IGAR Y  
Sbjct: 130 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTP 189

Query: 198 RSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAVYKVCW 257
           +    P   R   D  GHG+HTAS  AG+ V   S YGLG GT RGGVP+ARIAVYKVC 
Sbjct: 190 KLEGFPESAR---DNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCD 249

Query: 258 SD--GCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGILTSNS 317
                C+   ILAAFDDAIAD VDI++VSLG      +   +++IGAFHAM  GILT N 
Sbjct: 250 PGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNG 309

Query: 318 AGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD-DKKNLSLV 377
           AGN+GP R T  S+APW+ +VAAS M+R F+T V LGN     G ++N+ D + K   LV
Sbjct: 310 AGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLV 369

Query: 378 YGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLMQGYSS 437
           YG    +    SS   +C    ++ K V+GKI++CD         + G  A ++   Y  
Sbjct: 370 YGKSASSRCDASSAG-FCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPYED 429

Query: 438 SARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRGPNSLT 497
           +A  +  P S+L+  D   V  Y+ ST+ P A + KS TI +  AP V S+SSRGPN L 
Sbjct: 430 AASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLI 489

Query: 498 SDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVKTFH 557
            DILKPD++APG EILAA+ P  P +   D+R V Y +ISGTSMSCPHV  +AAY+KTFH
Sbjct: 490 HDILKPDITAPGSEILAAYSPYVPPS-ESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFH 549

Query: 558 PTWSPAAIKSALMTTASPMKEKI---NKEAEFAYGSGQVDPLKAIDPGLVYDAKESDYVK 617
           P WSP+ I+SA+MTTA PM       N+ AEFAYG+G VDP+ AI PGLVY+A +SD++ 
Sbjct: 550 PLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHIT 609

Query: 618 FLCGEGYNTTMVRRITGDNSACTSANIGRVW-DLNYPSFALSVARSQISNINQNFLRTVT 677
           FLCG  Y    +R I+GD+S+CT      +  +LNYPS +  V+ ++   +   F RTVT
Sbjct: 610 FLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKV--TFRRTVT 669

Query: 678 SVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGA--MTESRISASLVW 737
           +V    +T++A + G++ L + V P+VLS   + ++KSFT+TV GA    E+ +SA L+W
Sbjct: 670 NVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIW 729

BLAST of Tan0017682 vs. ExPASy Swiss-Prot
Match: Q9FIM5 (Subtilisin-like protease SBT4.9 OS=Arabidopsis thaliana OX=3702 GN=SBT4.9 PE=2 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 1.3e-166
Identity = 337/739 (45.60%), Postives = 459/739 (62.11%), Query Frame = 0

Query: 13  FNLVFLSLFSGLLA-SGFDSDDDGRKIYIVYMGS--KLKDPDSAHLHHRAMLEEVVGSNF 72
           F L+   L S +++ S    D   +++Y+VYMGS    +   +   HH ++L+EV G + 
Sbjct: 8   FCLISCVLVSFVISVSAVTDDSQDKQVYVVYMGSLPSSRLEYTPMSHHMSILQEVTGESS 67

Query: 73  APESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSFP--L 132
               ++ +YKRSFNGFA +L E E +++A  EGVVSVFP    +L TT SWDFL      
Sbjct: 68  VEGRLVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGK 127

Query: 133 YVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGAR 192
              R   +ES+TI+G +DSGIW +  SFSD+G+ PPP KWKG+C A  N +CN K+IGAR
Sbjct: 128 NTKRNLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFTCNNKLIGAR 187

Query: 193 AYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAVY 252
            Y +  +          D  GHGTHTAST AG+ V   S YG+G GTARGGVP++RIA Y
Sbjct: 188 DYTNEGTR---------DIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAY 247

Query: 253 KVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGILTS 312
           K C   GC+   +L+AFDDAIADGVD++S+SLG    + Y    I+IGAFHAM  GILT 
Sbjct: 248 KACSEMGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVKGILTV 307

Query: 313 NSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTADDKKNLSL 372
            SAGN GP   +  S+APWIL+VAAS  +R FVT V LGN     G ++N  D K     
Sbjct: 308 QSAGNGGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNYP 367

Query: 373 VYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLMQGYS 432
           +YGG                  S +  L+RGKILV +  + ++  ++       + + Y 
Sbjct: 368 LYGG------------------STDGPLLRGKILVSEDKVSSEIVVA------NINENYH 427

Query: 433 SSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRGPNSL 492
             A    LPSS L+  D  +V  Y+ ST+ P  T+ KS  I + +AP V  FSSRGPN++
Sbjct: 428 DYAYVSILPSSALSKDDFDSVISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTI 487

Query: 493 TSDILKPDLSAPGVEILAAWPPI-APITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVKT 552
             DILKPD++APGVEILAA+ P+ +P    +D+R V Y+++SGTSMSCPHV  +AAY+KT
Sbjct: 488 AVDILKPDVTAPGVEILAAFSPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGVAAYIKT 547

Query: 553 FHPTWSPAAIKSALMTTASPMKEKINKEA--EFAYGSGQVDPLKAIDPGLVYDAKESDYV 612
           FHP WSP+ I+SA+MTTA PM       A  EFAYG+G VDP+ AI+PGLVY+  +SD++
Sbjct: 548 FHPEWSPSMIQSAIMTTAWPMNATGTAVASTEFAYGAGHVDPIAAINPGLVYEIGKSDHI 607

Query: 613 KFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFLRTVT 672
            FLCG  YN T ++ I G+   CT   + R  +LNYPS +  + +S+ S I   F RTVT
Sbjct: 608 AFLCGLNYNATSLKLIAGEAVTCTGKTLPR--NLNYPSMSAKLPKSESSFI-VTFNRTVT 667

Query: 673 SVVSGKSTFRATI--SGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRI--SASL 732
           +V +  ST+++ I  +    L + VSPSVLS   + +++SFT+TV G+  + ++  SA+L
Sbjct: 668 NVGTPNSTYKSKIVLNHGSNLKVEVSPSVLSMKSVKEKQSFTVTVSGSNIDPKLPSSANL 710

Query: 733 VWSNGLFKVRSPITVYTRS 740
           +WS+G   VRSPI VYT S
Sbjct: 728 IWSDGTHNVRSPIVVYTYS 710

BLAST of Tan0017682 vs. NCBI nr
Match: XP_038891121.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 544/734 (74.11%), Postives = 613/734 (83.51%), Query Frame = 0

Query: 7   MSCSLIFNLVFLSLFSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAMLEEVVG 66
           MS SLI  L+ L+LF  LL+S  D DDDGRKIYIVYMGSKL+DPDSAHLHHRAMLEEVVG
Sbjct: 1   MSSSLILKLILLNLFFSLLSSNSDFDDDGRKIYIVYMGSKLEDPDSAHLHHRAMLEEVVG 60

Query: 67  SNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSFP 126
           S+F PES+IY YKRSFNGFAVKL EEEAQKIATKEGVVSVFPSE+NQLHTTRSWDFL FP
Sbjct: 61  SHFPPESLIYIYKRSFNGFAVKLTEEEAQKIATKEGVVSVFPSEKNQLHTTRSWDFLGFP 120

Query: 127 LYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGA 186
             V R+N+VESN IVGV D+GIWSDHPSFSD+GY PPPAKWKGICQ + N +CN+KIIGA
Sbjct: 121 SNVARMNKVESNIIVGVFDTGIWSDHPSFSDKGYGPPPAKWKGICQTAANFTCNKKIIGA 180

Query: 187 RAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAV 246
           R YRS  +LPPGDIRSPIDT+GHGTHTASTVAG L+ KASM GLGLGTARGGVPSARIAV
Sbjct: 181 RVYRSNKTLPPGDIRSPIDTDGHGTHTASTVAGGLMTKASMNGLGLGTARGGVPSARIAV 240

Query: 247 YKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGILT 306
           YKVCWSD C+DAD+LAAFDDAIADGVDI+S+S+GG  P+PYF H+ISIGAFHA+K GILT
Sbjct: 241 YKVCWSDECADADVLAAFDDAIADGVDIISLSVGGKLPKPYFQHTISIGAFHALKRGILT 300

Query: 307 SNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTADDKKN-L 366
           SNSAGNSGP   TT SL+PW+LSVAAST+DRKFVT VQLGN N  QGI++N  DD KN  
Sbjct: 301 SNSAGNSGPDPYTTASLSPWLLSVAASTIDRKFVTQVQLGNKNNFQGISVNAFDDLKNQY 360

Query: 367 SLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLMQG 426
            L+YGGDVPN GF SS SR+C+ NSVNP LV+GKILVCDA + +K F S G  AGVLMQ 
Sbjct: 361 PLIYGGDVPNKGFNSSSSRFCKENSVNPSLVKGKILVCDAQLSSKKFASMGSPAGVLMQA 420

Query: 427 YSSSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRGPN 486
           Y   A SYPLP+S L   DG+ +K YM+ST+ PTAT+FKSTTI DASAP V SFSSRGPN
Sbjct: 421 YRDYAVSYPLPASTLKLEDGSKLKSYMSSTKTPTATIFKSTTIQDASAPFVASFSSRGPN 480

Query: 487 SLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVK 546
           ++T DILKPD+SAPG EILAAWP  APITV QDSRR+LYNIISGTSM+CPHVTAIA Y+K
Sbjct: 481 AITHDILKPDVSAPGAEILAAWPLNAPITVAQDSRRLLYNIISGTSMACPHVTAIATYIK 540

Query: 547 TFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAKESDYVK 606
           TF+PTWSPAAIKSALMTTASPM   IN +AEFAYGSGQV+PLKA+ PGLVYDA ESDY++
Sbjct: 541 TFNPTWSPAAIKSALMTTASPMNPSINDDAEFAYGSGQVNPLKAVKPGLVYDANESDYIE 600

Query: 607 FLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFLRTVTS 666
           FLCG+GY T M+R ITGDNS CTS+  G VWDLNYPSFALSV RSQ   INQ+F RT+T+
Sbjct: 601 FLCGQGYTTAMLRYITGDNSTCTSS--GLVWDLNYPSFALSVTRSQ-RFINQHFTRTLTN 660

Query: 667 VVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISASLVWSNG 726
           V  G ST++AT+S  QGL I+V+PS LSF   GD+K F LTVQG + +  IS SL WS+G
Sbjct: 661 VEKGGSTYKATVSSPQGLLISVNPSTLSFKATGDKKPFALTVQGTVRQPIISGSLTWSDG 720

Query: 727 LFKVRSPITVYTRS 740
              VRSPI VY RS
Sbjct: 721 RHSVRSPIIVYVRS 731

BLAST of Tan0017682 vs. NCBI nr
Match: AEM42989.1 (cucumisin [Siraitia grosvenorii])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.8e-306
Identity = 536/737 (72.73%), Postives = 620/737 (84.12%), Query Frame = 0

Query: 1   MIRSITMSCSLIFNLVFLSLFSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAM 60
           M RS TMS SL+F L+FLSLF  LL S  DS+DDGRKIYIVYMGSKL+D  SAHL+HRAM
Sbjct: 1   MTRS-TMS-SLVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAM 60

Query: 61  LEEVVGSNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSW 120
           LEEVVGS FAPESVIYTYKRSFNGFAVKL EEEA KIA KEGVVSVFPSE+N LHTTRSW
Sbjct: 61  LEEVVGSTFAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSW 120

Query: 121 DFLSFPLYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCN 180
           DFL     VPRV +VESN +VGV DSGIW ++PSF+D G+ P PA W+G CQASTN  CN
Sbjct: 121 DFLGISQNVPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCN 180

Query: 181 RKIIGARAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVP 240
           RKIIGARAYRS S+LPPGD+RSP DT+GHGTHTASTVAG LV++AS+YGLG+GTARGGVP
Sbjct: 181 RKIIGARAYRS-STLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVP 240

Query: 241 SARIAVYKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAM 300
            ARIAVYK+CWSDGCSDADILAAFDDAIADGVDI+S+S+GG  PQPY  +SI+IG+FHAM
Sbjct: 241 PARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAM 300

Query: 301 KHGILTSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD 360
           K GILTSNSAGN+GP   T TSL+PW+ +VAAS+ DRKFVT V LGN N +QG++INT D
Sbjct: 301 KRGILTSNSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFD 360

Query: 361 DKKNLSLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAG 420
            +    L+Y G+ P++GF SS SRYC  +SV+P LVRGKIL+CD+      F SFG AAG
Sbjct: 361 MRNQYPLIYAGNAPSIGFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAG 420

Query: 421 VLMQGYS-SSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISF 480
           VLMQ  +   A SYPLP+S+L+P  G N+KRYM+STR PTAT+FKST + D SAP V+SF
Sbjct: 421 VLMQSNTRDHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSF 480

Query: 481 SSRGPNSLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTA 540
           SSRGPN +T DILKPD +APGVEILAAWPP+API+  +DSR  LYNIISGTSMSCPHVTA
Sbjct: 481 SSRGPNYVTHDILKPDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVTA 540

Query: 541 IAAYVKTFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAK 600
           IA ++KTF+P+WSPAAIKSALMTTASPM  + N +AEFAYGSG V+PLKA+DPGLVYDA 
Sbjct: 541 IAVHIKTFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDAS 600

Query: 601 ESDYVKFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNF 660
           ESDYVKFLCGEGY T MVR  TGDNSACTS NIGRVWDLNYPSFALS++RSQ +  NQ+F
Sbjct: 601 ESDYVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQTA--NQSF 660

Query: 661 LRTVTSVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISAS 720
            RT+T+VVSG ST+RA+IS  QGL+I+V+PSVLSFNGIGDQKSFTLTV+G ++++ +SAS
Sbjct: 661 RRTLTNVVSGASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVSQAIVSAS 720

Query: 721 LVWSNGLFKVRSPITVY 737
           LVWS+G   VRSPITVY
Sbjct: 721 LVWSDGSHNVRSPITVY 732

BLAST of Tan0017682 vs. NCBI nr
Match: BBK45496.1 (pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 trichocucumisin [Trichosanthes bracteata])

HSP 1 Score: 1061.6 bits (2744), Expect = 3.1e-306
Identity = 530/735 (72.11%), Postives = 621/735 (84.49%), Query Frame = 0

Query: 1   MIRSITMSCSLIFNLVFLSLFSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAM 60
           MIRSITMS S+IF LV LSL S LLASGFDSDDDGRKIYIVYMG+KL+DPDSAHLHHRAM
Sbjct: 1   MIRSITMS-SVIFKLVLLSLLSSLLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAM 60

Query: 61  LEEVVGSNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSW 120
           LEEVVGS FAPESVIYTYKRSFNGFAVKL EEEAQKIA KEGVVSVF +++N +HTT SW
Sbjct: 61  LEEVVGSTFAPESVIYTYKRSFNGFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSW 120

Query: 121 DFLSFPLYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCN 180
           DFL FP  V R+ +VESN +VGVLDSGIW + PSF D+G+   P+ WKG CQAS N SCN
Sbjct: 121 DFLGFPQAVARMRQVESNIVVGVLDSGIWPESPSFRDEGFGRAPSSWKGTCQASKNFSCN 180

Query: 181 RKIIGARAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVP 240
            KIIGARAYR   +LPPGD+ SP DT+GHGTHTASTVAG LV++AS+YGLGLGTARGGVP
Sbjct: 181 NKIIGARAYRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVP 240

Query: 241 SARIAVYKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAM 300
           SARIA YK+CWSDGCSDADILAAFDDAIADGV I+S+S+GG++ +PYF   I+IGAFHAM
Sbjct: 241 SARIAAYKICWSDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAM 300

Query: 301 KHGILTSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD 360
           KHGILTSNSAGN G    TTTSL+PW+LSVAAST DRKFVT VQLGN  I+QG AINT D
Sbjct: 301 KHGILTSNSAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFD 360

Query: 361 DKKNLSLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAG 420
                 LVY G+VPNVGF  S SR+C  NSVNP LV+GKIL+CD+++P+  F SFG  AG
Sbjct: 361 MNNQYPLVYAGEVPNVGFNGSTSRFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAG 420

Query: 421 VLMQGYSSSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFS 480
           VLMQGY+ +ARS+ +P+++L+P DG NV RY+ ST  PTA +   TT+ DASAP VISFS
Sbjct: 421 VLMQGYTDNARSHSVPATVLHPQDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISFS 480

Query: 481 SRGPNSLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAI 540
           SRGPN+LT+D+LKPD++ PGVEILAAWPPIAPI+  ++SR  L+NIISGTSMSCPH++A+
Sbjct: 481 SRGPNALTNDLLKPDITGPGVEILAAWPPIAPISGVRESRSTLFNIISGTSMSCPHLSAV 540

Query: 541 AAYVKTFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAKE 600
           A YVKTF+P+WSPAAIKSALMTTASPM  ++N EAEFAYGSG V+PLKA++PGLVYDA +
Sbjct: 541 AVYVKTFNPSWSPAAIKSALMTTASPMTARLNSEAEFAYGSGHVNPLKAVNPGLVYDADQ 600

Query: 601 SDYVKFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFL 660
           SDYVKFLCG+GYNT M+RRITGDNSACTSANIGRVWDLNYPSFAL+V+  Q  ++NQ F 
Sbjct: 601 SDYVKFLCGQGYNTAMLRRITGDNSACTSANIGRVWDLNYPSFALAVSGYQ--SLNQYFT 660

Query: 661 RTVTSVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISASL 720
           RT+T+V S  ST+RATIS  QGLTITV+P VLSF+G GD+KSFTLTV+G++++  +S SL
Sbjct: 661 RTLTNVASQASTYRATISVPQGLTITVNPPVLSFSGKGDRKSFTLTVRGSVSQPIVSGSL 720

Query: 721 VWSNGLFKVRSPITV 736
           VWS+G+  VRSP+ V
Sbjct: 721 VWSDGVHSVRSPLVV 732

BLAST of Tan0017682 vs. NCBI nr
Match: XP_023527145.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1008.1 bits (2605), Expect = 4.0e-290
Identity = 505/734 (68.80%), Postives = 601/734 (81.88%), Query Frame = 0

Query: 3   RSITMSCSLIFNLVFLSLFSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAMLE 62
           + ITM  SL+FNL+FL+LF  +LAS   S  D RKIYIVYMGSKL+DP SAHLHHRAMLE
Sbjct: 4   KGITMCSSLLFNLLFLALFRSVLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLE 63

Query: 63  EVVGSNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDF 122
           +VVGS F P+SV+YTY RSFNGF V+L EEEAQ+IA+KEGVVSVF ++ N LHTTRSWDF
Sbjct: 64  QVVGSTFDPQSVLYTYTRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDF 123

Query: 123 LSFPLYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRK 182
           L FP  V RV ++ESN +VGVLDSGIW + PSF+D G+  PP+KW+G CQ S N  CNRK
Sbjct: 124 LGFPQNVGRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGSCQVS-NFRCNRK 183

Query: 183 IIGARAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSA 242
           +IG RAYR    + PGD+ SP DT+GHGTHTASTVAG LV++AS+YGLG+GTARGGVPSA
Sbjct: 184 LIGGRAYRIGRPILPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSA 243

Query: 243 RIAVYKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKH 302
           RIA YKVCW DGCSDADILAAFDDAIADGVDI+S+S+G + P+PYF   I+IG+FHA+K+
Sbjct: 244 RIAAYKVCWKDGCSDADILAAFDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKN 303

Query: 303 GILTSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTADDK 362
           GILTSNSAGN GP   TTTSL+PW+LSVAAS MDRKFVT VQLGN NI++GI+INT D  
Sbjct: 304 GILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMN 363

Query: 363 KNLSLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVL 422
               LVY GD+PN+GF  S SRYCE NSVNP L  GKIL+CD+V+P+  F SFG  AG+L
Sbjct: 364 LQYPLVYAGDIPNLGFNGSTSRYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGIL 423

Query: 423 MQGYS-SSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSS 482
           MQ Y+  +ARSYP P+++L+P DG  V RY+ ST  PTAT+FKS    D+SAP V+SFSS
Sbjct: 424 MQAYTRDNARSYPAPAAVLHPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSS 483

Query: 483 RGPNSLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIA 542
           RGPN+LT+DILKPDLS PGVEILAAWPP+API   +DSR  LYNIISGTSMSCPH+T +A
Sbjct: 484 RGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVA 543

Query: 543 AYVKTFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAKES 602
            YVKTF+PT SPAAIKSAL+TTASPM  + + +AEFAYG+G V+PLKAI+PGLVYDA ES
Sbjct: 544 VYVKTFNPTLSPAAIKSALITTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANES 603

Query: 603 DYVKFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFLR 662
           DYV FLCG+GY+T+MVRRITGDNSACTS NIGRVWDLNYPSFA+S++ ++   +NQ F R
Sbjct: 604 DYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTR--TVNQYFTR 663

Query: 663 TVTSVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISASLV 722
           T+T+V  G ST+RA IS  QGL+ITV+P+ LSFNGIGD+KSF LTV G +++S  SASL 
Sbjct: 664 TLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLE 723

Query: 723 WSNGLFKVRSPITV 736
           WS+G+  VRSPITV
Sbjct: 724 WSDGVHSVRSPITV 734

BLAST of Tan0017682 vs. NCBI nr
Match: KAG6600967.1 (hypothetical protein SDJN03_06200, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1008.1 bits (2605), Expect = 4.0e-290
Identity = 505/730 (69.18%), Postives = 599/730 (82.05%), Query Frame = 0

Query: 7   MSCSLIFNLVFLSLFSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAMLEEVVG 66
           M  SL+ NL+FL+LF  +LAS   S DD RKIYIVYMGSKL+DP SAHLHHRAMLE+VVG
Sbjct: 1   MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60

Query: 67  SNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSFP 126
           S F P+SV+YTY RSFNGF V+L EEEAQ+IA+KEGVVSVF ++ N LHTTRSWDF+ FP
Sbjct: 61  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120

Query: 127 LYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGA 186
             V RV ++ESN +VGVLDSGIW + PSF+D G+  PP+KW+G CQ S N SCNRK+IG 
Sbjct: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDS-NFSCNRKLIGG 180

Query: 187 RAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAV 246
           RAY     +PPGD+ SP DT+GHGTHTASTVAG LV++AS+YGLGLGTARGGVPSARIA 
Sbjct: 181 RAYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAA 240

Query: 247 YKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGILT 306
           YKVCW DGCSDADILAAFDDAIADGVDI+S+S+G ++P+PYF   I+IG+FHA+K+GILT
Sbjct: 241 YKVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILT 300

Query: 307 SNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTADDKKNLS 366
           SNSAGN GP   TTTSL+PW+LSVAAS MDRKFVT VQLGN NI+QGI+INT +      
Sbjct: 301 SNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHP 360

Query: 367 LVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLMQGY 426
           LVY GD+PN+GF  S SRYCE NSVNP L  GKIL+CD+V+P   F SFG  AG+LMQ Y
Sbjct: 361 LVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAY 420

Query: 427 S-SSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRGPN 486
           +  +ARSYP P+++L+P DG  V RY+ ST  PTAT+FKS    D+SAP V+SFSSRGPN
Sbjct: 421 TRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPN 480

Query: 487 SLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVK 546
           +LT+DILKPDLS PGVEILAAWPP+API   +DSR  LYNIISGTSMSCPH+T +A YVK
Sbjct: 481 ALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVK 540

Query: 547 TFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAKESDYVK 606
           TF+PT SPAAIKSALMTTASPM  + + +AEFAYG+G V+PLKAI+PGLVYDA ESDYV 
Sbjct: 541 TFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVS 600

Query: 607 FLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFLRTVTS 666
           FLCG+GY+T+MVRRITGDNSACTS NIGRVWDLNYPSFA+S++ ++   +NQ F RT+T+
Sbjct: 601 FLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTR--TVNQYFTRTLTN 660

Query: 667 VVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISASLVWSNG 726
           V  G ST+RA IS  QGL+ITV+P+ LSFNGIGD+KSF LTV G +++S  SASL WS+G
Sbjct: 661 VARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDG 720

Query: 727 LFKVRSPITV 736
           +  VRSPITV
Sbjct: 721 VHSVRSPITV 727

BLAST of Tan0017682 vs. ExPASy TrEMBL
Match: K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 8.7e-307
Identity = 536/737 (72.73%), Postives = 620/737 (84.12%), Query Frame = 0

Query: 1   MIRSITMSCSLIFNLVFLSLFSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAM 60
           M RS TMS SL+F L+FLSLF  LL S  DS+DDGRKIYIVYMGSKL+D  SAHL+HRAM
Sbjct: 1   MTRS-TMS-SLVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAM 60

Query: 61  LEEVVGSNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSW 120
           LEEVVGS FAPESVIYTYKRSFNGFAVKL EEEA KIA KEGVVSVFPSE+N LHTTRSW
Sbjct: 61  LEEVVGSTFAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSW 120

Query: 121 DFLSFPLYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCN 180
           DFL     VPRV +VESN +VGV DSGIW ++PSF+D G+ P PA W+G CQASTN  CN
Sbjct: 121 DFLGISQNVPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCN 180

Query: 181 RKIIGARAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVP 240
           RKIIGARAYRS S+LPPGD+RSP DT+GHGTHTASTVAG LV++AS+YGLG+GTARGGVP
Sbjct: 181 RKIIGARAYRS-STLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVP 240

Query: 241 SARIAVYKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAM 300
            ARIAVYK+CWSDGCSDADILAAFDDAIADGVDI+S+S+GG  PQPY  +SI+IG+FHAM
Sbjct: 241 PARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAM 300

Query: 301 KHGILTSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD 360
           K GILTSNSAGN+GP   T TSL+PW+ +VAAS+ DRKFVT V LGN N +QG++INT D
Sbjct: 301 KRGILTSNSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFD 360

Query: 361 DKKNLSLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAG 420
            +    L+Y G+ P++GF SS SRYC  +SV+P LVRGKIL+CD+      F SFG AAG
Sbjct: 361 MRNQYPLIYAGNAPSIGFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAG 420

Query: 421 VLMQGYS-SSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISF 480
           VLMQ  +   A SYPLP+S+L+P  G N+KRYM+STR PTAT+FKST + D SAP V+SF
Sbjct: 421 VLMQSNTRDHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSF 480

Query: 481 SSRGPNSLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTA 540
           SSRGPN +T DILKPD +APGVEILAAWPP+API+  +DSR  LYNIISGTSMSCPHVTA
Sbjct: 481 SSRGPNYVTHDILKPDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVTA 540

Query: 541 IAAYVKTFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAK 600
           IA ++KTF+P+WSPAAIKSALMTTASPM  + N +AEFAYGSG V+PLKA+DPGLVYDA 
Sbjct: 541 IAVHIKTFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDAS 600

Query: 601 ESDYVKFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNF 660
           ESDYVKFLCGEGY T MVR  TGDNSACTS NIGRVWDLNYPSFALS++RSQ +  NQ+F
Sbjct: 601 ESDYVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQTA--NQSF 660

Query: 661 LRTVTSVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISAS 720
            RT+T+VVSG ST+RA+IS  QGL+I+V+PSVLSFNGIGDQKSFTLTV+G ++++ +SAS
Sbjct: 661 RRTLTNVVSGASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVSQAIVSAS 720

Query: 721 LVWSNGLFKVRSPITVY 737
           LVWS+G   VRSPITVY
Sbjct: 721 LVWSDGSHNVRSPITVY 732

BLAST of Tan0017682 vs. ExPASy TrEMBL
Match: A0A4P2YW59 (Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2 SV=1)

HSP 1 Score: 1061.6 bits (2744), Expect = 1.5e-306
Identity = 530/735 (72.11%), Postives = 621/735 (84.49%), Query Frame = 0

Query: 1   MIRSITMSCSLIFNLVFLSLFSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAM 60
           MIRSITMS S+IF LV LSL S LLASGFDSDDDGRKIYIVYMG+KL+DPDSAHLHHRAM
Sbjct: 1   MIRSITMS-SVIFKLVLLSLLSSLLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAM 60

Query: 61  LEEVVGSNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSW 120
           LEEVVGS FAPESVIYTYKRSFNGFAVKL EEEAQKIA KEGVVSVF +++N +HTT SW
Sbjct: 61  LEEVVGSTFAPESVIYTYKRSFNGFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSW 120

Query: 121 DFLSFPLYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCN 180
           DFL FP  V R+ +VESN +VGVLDSGIW + PSF D+G+   P+ WKG CQAS N SCN
Sbjct: 121 DFLGFPQAVARMRQVESNIVVGVLDSGIWPESPSFRDEGFGRAPSSWKGTCQASKNFSCN 180

Query: 181 RKIIGARAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVP 240
            KIIGARAYR   +LPPGD+ SP DT+GHGTHTASTVAG LV++AS+YGLGLGTARGGVP
Sbjct: 181 NKIIGARAYRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVP 240

Query: 241 SARIAVYKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAM 300
           SARIA YK+CWSDGCSDADILAAFDDAIADGV I+S+S+GG++ +PYF   I+IGAFHAM
Sbjct: 241 SARIAAYKICWSDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAM 300

Query: 301 KHGILTSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD 360
           KHGILTSNSAGN G    TTTSL+PW+LSVAAST DRKFVT VQLGN  I+QG AINT D
Sbjct: 301 KHGILTSNSAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFD 360

Query: 361 DKKNLSLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAG 420
                 LVY G+VPNVGF  S SR+C  NSVNP LV+GKIL+CD+++P+  F SFG  AG
Sbjct: 361 MNNQYPLVYAGEVPNVGFNGSTSRFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAG 420

Query: 421 VLMQGYSSSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFS 480
           VLMQGY+ +ARS+ +P+++L+P DG NV RY+ ST  PTA +   TT+ DASAP VISFS
Sbjct: 421 VLMQGYTDNARSHSVPATVLHPQDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISFS 480

Query: 481 SRGPNSLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAI 540
           SRGPN+LT+D+LKPD++ PGVEILAAWPPIAPI+  ++SR  L+NIISGTSMSCPH++A+
Sbjct: 481 SRGPNALTNDLLKPDITGPGVEILAAWPPIAPISGVRESRSTLFNIISGTSMSCPHLSAV 540

Query: 541 AAYVKTFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAKE 600
           A YVKTF+P+WSPAAIKSALMTTASPM  ++N EAEFAYGSG V+PLKA++PGLVYDA +
Sbjct: 541 AVYVKTFNPSWSPAAIKSALMTTASPMTARLNSEAEFAYGSGHVNPLKAVNPGLVYDADQ 600

Query: 601 SDYVKFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFL 660
           SDYVKFLCG+GYNT M+RRITGDNSACTSANIGRVWDLNYPSFAL+V+  Q  ++NQ F 
Sbjct: 601 SDYVKFLCGQGYNTAMLRRITGDNSACTSANIGRVWDLNYPSFALAVSGYQ--SLNQYFT 660

Query: 661 RTVTSVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISASL 720
           RT+T+V S  ST+RATIS  QGLTITV+P VLSF+G GD+KSFTLTV+G++++  +S SL
Sbjct: 661 RTLTNVASQASTYRATISVPQGLTITVNPPVLSFSGKGDRKSFTLTVRGSVSQPIVSGSL 720

Query: 721 VWSNGLFKVRSPITV 736
           VWS+G+  VRSP+ V
Sbjct: 721 VWSDGVHSVRSPLVV 732

BLAST of Tan0017682 vs. ExPASy TrEMBL
Match: A0A6J1GZY5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 5.3e-288
Identity = 502/730 (68.77%), Postives = 594/730 (81.37%), Query Frame = 0

Query: 7   MSCSLIFNLVFLSLFSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAMLEEVVG 66
           MS  LI NL+FL+LF  +LAS   S DD R+IYIVYMGSKL+DP SAHLHHRAMLE+VVG
Sbjct: 8   MSSPLILNLLFLALFCSVLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67

Query: 67  SNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSFP 126
           S F P+SV+YTY RSFNGF V L EEEAQ+IA+KEGVVSVF ++ N LHTTRSWDF+ FP
Sbjct: 68  STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 127

Query: 127 LYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGA 186
             V RV ++ESN +VGVLDSGIW + PSF+D G+  PP+KW+G CQ S N SCNRK+IG 
Sbjct: 128 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFSCNRKLIGG 187

Query: 187 RAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAV 246
           RAY     + PGD+ SP DT+GHGTHTASTVAG LV++AS+YGLGLGTARGGVPSARIA 
Sbjct: 188 RAYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAA 247

Query: 247 YKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGILT 306
           YKVCW DGC DADILAAFDDAIADGVDI+S+S+G  +P+PYF   I+IG+FHA+K+GILT
Sbjct: 248 YKVCWEDGCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSFHAIKNGILT 307

Query: 307 SNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTADDKKNLS 366
           SNSAGN GP   TTTSL+PW+LSVAAS MDRKFVT VQLGN NI+QGI+INT +      
Sbjct: 308 SNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHP 367

Query: 367 LVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLMQGY 426
           LVY GD+PN+GF  S SRYCE NSVNP L  GKIL+CD+V+P   F SFG   G+LMQ Y
Sbjct: 368 LVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFFSFGAIVGILMQAY 427

Query: 427 S-SSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRGPN 486
           +  +ARSYP P+++L+P DG  V RY+ ST  PTAT+FKS    D+SAP V+SFSSRGPN
Sbjct: 428 TRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPN 487

Query: 487 SLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVK 546
           +LT+DILKPDLS PGVEILAAWPP+API   +DSR  LYNIISGTSMSCPH+T +A YVK
Sbjct: 488 ALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVK 547

Query: 547 TFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAKESDYVK 606
           TF+PT SPAAIKSALMTTASPM  + + +AEFAYG+G V+PLKAI+PGLVYDA ESDYV 
Sbjct: 548 TFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVS 607

Query: 607 FLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFLRTVTS 666
           FLCG+GY+T MVRRITGDNSACTS NIGRVWDLNYPSFA+S++ ++   +NQ F RT+T+
Sbjct: 608 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTR--TVNQYFTRTLTN 667

Query: 667 VVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISASLVWSNG 726
           V  G ST+RA IS  QGL+ITV+P+ LSFNGIGD+KSF LTV+G +++S  SASL WS+G
Sbjct: 668 VARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRGTVSQSISSASLEWSDG 727

Query: 727 LFKVRSPITV 736
           +  VRSPITV
Sbjct: 728 VHSVRSPITV 734

BLAST of Tan0017682 vs. ExPASy TrEMBL
Match: A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 2.6e-279
Identity = 497/732 (67.90%), Postives = 583/732 (79.64%), Query Frame = 0

Query: 7   MSCSLIFNLVFLSL-FSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHLHHRAMLEEVV 66
           MS SLIF L F SL FS  LAS  DSDDDG+ IYIVYMG KL+DPDSAHLHHRAMLE+VV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 67  GSNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSF 126
           GS FAPESV++TYKRSFNGFAVKL EEEA+KIA+ EGVVSVF +E N+LHTTRSWDFL F
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 127 PLYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIG 186
           PL VPR ++VESN +VGVLD+GIW + PSF D+G+ PPP KWKG C+ S N  CNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 187 ARAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIA 246
           AR+Y     + PGD+  P DTNGHGTHTAST AG LV++A++YGLGLGTARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 247 VYKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGIL 306
            YKVCW+DGCSDADILAA+DDAIADGVDI+S+S+GG  P+ YF+ +I+IG+FHA++ GIL
Sbjct: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 307 TSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTADDKKNL 366
           TSNSAGN GP   TT SL+PW+LSVAASTMDRKFVT VQ+GN    QG++INT D+ +  
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDN-QYY 360

Query: 367 SLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVM-PAKAFLSFGEAAGVLMQ 426
            LV G D+PN GF  S SR+C  NSV PKL++GKI+VC+A   P + F S   AAGVLM 
Sbjct: 361 PLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMT 420

Query: 427 GYSSS-ARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRG 486
             +   A SYPLPSS+L+P D     RY+ S R P AT+FKSTTI +ASAP V+SFSSRG
Sbjct: 421 SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRG 480

Query: 487 PNSLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAY 546
           PN  T D++KPD+S PGVEILAAWP +AP  V    R  L+NIISGTSMSCPH+T IA Y
Sbjct: 481 PNRATKDVIKPDISGPGVEILAAWPSVAP--VGGIRRNTLFNIISGTSMSCPHITGIATY 540

Query: 547 VKTFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAKESDY 606
           VKT++PTWSPAAIKSALMTTASPM  + N +AEFAYGSG V+PLKA+ PGLVYDA ESDY
Sbjct: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600

Query: 607 VKFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFLRTV 666
           VKFLCG+GYNT  VRRITGD SACT  N GRVWDLNYPSF LSV+ S+    NQ F RT+
Sbjct: 601 VKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSK--TFNQYFNRTL 660

Query: 667 TSVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISASLVWS 726
           TSV    ST+RA IS  QGLTI+V+P+VLSFNG+GD+KSFTLTV+G++    +SASLVWS
Sbjct: 661 TSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWS 720

Query: 727 NGLFKVRSPITV 736
           +G+  VRSPIT+
Sbjct: 721 DGVHSVRSPITI 727

BLAST of Tan0017682 vs. ExPASy TrEMBL
Match: A0A6J1CEK6 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010021 PE=3 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 3.5e-279
Identity = 500/737 (67.84%), Postives = 584/737 (79.24%), Query Frame = 0

Query: 7   MSCSLIFNLVFLSLFSGLLASGFDS-DDDGRKIYIVYMGSKLKDPDSAHLHHRAMLEEVV 66
           MS SLIF LVFLSL  GLLAS  DS +DDGRKIYIVY+G+K +D  S   HH  MLEEVV
Sbjct: 1   MSSSLIFKLVFLSLLCGLLASSLDSNNDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEVV 60

Query: 67  GSNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSF 126
           GS FAPE+++++YKRSFNGF VKL EEEAQKIA KEGVVSVF + +  LHTTRSWDF+ F
Sbjct: 61  GSTFAPEALLHSYKRSFNGFVVKLTEEEAQKIAAKEGVVSVFLNGKKHLHTTRSWDFMGF 120

Query: 127 PLYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIG 186
              V RV +VESN +VGVLDSGIW + PSFSD GY PPP KWKG CQ S N  CN+KIIG
Sbjct: 121 TKNVARVKQVESNIVVGVLDSGIWPESPSFSDLGYGPPPVKWKGTCQTSANFHCNKKIIG 180

Query: 187 ARAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIA 246
           ARAYRS +  PP DIRSP D++GHGTHTASTVAG LVN+AS+YGL LGTARGGVPSARIA
Sbjct: 181 ARAYRSNNFFPPEDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 240

Query: 247 VYKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGIL 306
           VYK+CWSDGC DADILAAFDDAIADGVDI+S+S+GG+ P+ YF  SI+IGAFH+MKHGIL
Sbjct: 241 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGIL 300

Query: 307 TSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD-DKKN 366
           TSNSAGN GP   T  + +PW LSVAAS++DRKFVT VQLGN N++QG  INT D   K 
Sbjct: 301 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKFVTKVQLGNKNLYQGYTINTFDLLGKQ 360

Query: 367 LSLVYGGDVPNV--GFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVL 426
             L+YGGD PN+  GF SS SR+C  N+V+  LV+GKILVCD V+   AF SF  A GV+
Sbjct: 361 YPLIYGGDAPNITGGFTSSSSRFCSKNTVDRNLVKGKILVCDTVLSPSAFASFXGAVGVV 420

Query: 427 MQ--GYSSSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFS 486
           MQ  G   +ARSYPLP+S L    G ++K YM S R  TAT+ KS  + D SAP V+SFS
Sbjct: 421 MQDAGVKDNARSYPLPASYLGTAAGGSIKNYMASNRAATATILKSNAVNDTSAPLVVSFS 480

Query: 487 SRGPNSLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAI 546
           SRGPN  T DILKPDL+APGVEILAAW PIAPI+   DSR+ LYNIISGTSMSCPH TA+
Sbjct: 481 SRGPNPETFDILKPDLTAPGVEILAAWSPIAPISGITDSRKSLYNIISGTSMSCPHATAV 540

Query: 547 AAYVKTFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKAIDPGLVYDAKE 606
           A YVKTFHPTWSPAAIKSALMTTA+P+  K+N +AEFAYG+G V+PLKA++PGLVYDA E
Sbjct: 541 AVYVKTFHPTWSPAAIKSALMTTATPLNPKLNPQAEFAYGAGHVNPLKAVNPGLVYDAHE 600

Query: 607 SDYVKFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFL 666
           SDYVKFLCG+GY T MV+R+T D SACT AN  RVWDLNYPSFALS   S+  +INQ F 
Sbjct: 601 SDYVKFLCGQGYTTAMVQRLTADASACTPANTARVWDLNYPSFALSSTPSE--SINQFFT 660

Query: 667 RTVTSVVSGKSTFRATISGA-QGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRISAS 726
           RT+T+V S  ST++A + GA  GLTI+VSP VLSF+ IG++KSF LT++G ++ S +SAS
Sbjct: 661 RTLTNVGSKASTYKAAVYGAPPGLTISVSPPVLSFHAIGEKKSFKLTIRGTLSHSIVSAS 720

Query: 727 LVWSNGLFKVRSPITVY 737
           LVW++GL +VRSPITVY
Sbjct: 721 LVWNDGLHQVRSPITVY 735

BLAST of Tan0017682 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 599.4 bits (1544), Expect = 4.0e-171
Identity = 343/743 (46.16%), Postives = 470/743 (63.26%), Query Frame = 0

Query: 17  FLSLFSGLLASGF-----DSDDDG-RKIYIVYMGS-KLKDPDSAHLHHRAMLEEVVGSNF 76
           F+ LFS LL         D DD G +++YIVY+GS   ++  +    H ++L+E+ G + 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 77  APESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSFP--L 136
               ++ +YK+SFNGFA +L E E +++A  E VVSVFPS + +L TT SW+F+     +
Sbjct: 67  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126

Query: 137 YVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGAR 196
              R   +ES+TI+GV+DSGI+ +  SFSDQG+ PPP KWKG C    N +CN K+IGAR
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 186

Query: 197 AYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAVY 256
            Y ++S       ++  D +GHGTHTAS  AG+ V  ++ YGLG GTARGGVP+ARIAVY
Sbjct: 187 DYTAKSKAN----QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVY 246

Query: 257 KVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGILTS 316
           KVC ++GC    +++AFDDAIADGVD++S+S+      P+    I+IGAFHAM  G+LT 
Sbjct: 247 KVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTV 306

Query: 317 NSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD-DKKNLS 376
           N+AGN+GP  ST TS APW+ SVAAS  +R F+  V LG+  I  G ++NT D +  N  
Sbjct: 307 NAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYP 366

Query: 377 LVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLMQGY 436
           LVYG            +R CE   ++ KLV+GKI++CD+          G    ++    
Sbjct: 367 LVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPE 426

Query: 437 SSSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRGPNS 496
              A     P S L+  D  ++  YM ST+ P AT+ KS  I +  AP V SFSSRGP+S
Sbjct: 427 PDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSS 486

Query: 497 LTSDILKPDLSAPGVEILAAW-PPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVK 556
           + SDILKPD++APGVEILAA+ P  +P     D+RRV Y+++SGTSM+CPHV  +AAYVK
Sbjct: 487 IVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVK 546

Query: 557 TFHPTWSPAAIKSALMTTASPMKEKIN--KEAEFAYGSGQVDPLKAIDPGLVYDAKESDY 616
           TFHP WSP+ I+SA+MTTA PM    +     EFAYGSG VDP+ AI+PGLVY+  ++D+
Sbjct: 547 TFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADH 606

Query: 617 VKFLCGEGYNTTMVRRITGDNSACT---SANIGRVWDLNYPSFALSVARSQISNINQNFL 676
           + FLCG  Y +  +R I+GDNS CT   S  + R  +LNYP+ +  V+ ++  NI   F 
Sbjct: 607 INFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTKPFNI--TFQ 666

Query: 677 RTVTSVVSGKSTFRATISGAQG--LTITVSPSVLSFNGIGDQKSFTLTVQGAM--TESRI 736
           RTVT+V   KST+ A +    G  L+I VSP VLS   + +++SF +TV      T+  +
Sbjct: 667 RTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPV 726

Query: 737 SASLVWSNGLFKVRSPITVYTRS 740
           SA+L+WS+G   VRSPI VY  S
Sbjct: 727 SANLIWSDGTHNVRSPIIVYAMS 741

BLAST of Tan0017682 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 596.3 bits (1536), Expect = 3.4e-170
Identity = 341/734 (46.46%), Postives = 461/734 (62.81%), Query Frame = 0

Query: 18  LSLFSGLLASGFDS---DDDGRKIYIVYMGSKLKDPDSAHL-HHRAMLEEVVGSNFAPES 77
           LS    LL   F S   DD  +++YIVYMG+     D   + HH ++L++V G +   + 
Sbjct: 10  LSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQDR 69

Query: 78  VIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSFP--LYVPR 137
           ++  YKRSFNGFA +L E E + +A+ + VVSVFPS+   L TT SW+F+         R
Sbjct: 70  LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 129

Query: 138 VNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGARAYRS 197
              +ES+TI+GV+DSGI+ +  SFS +G+ PPP KWKG+C+  TN +CN K+IGAR Y  
Sbjct: 130 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYTP 189

Query: 198 RSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAVYKVCW 257
           +    P   R   D  GHG+HTAS  AG+ V   S YGLG GT RGGVP+ARIAVYKVC 
Sbjct: 190 KLEGFPESAR---DNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCD 249

Query: 258 SD--GCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGILTSNS 317
                C+   ILAAFDDAIAD VDI++VSLG      +   +++IGAFHAM  GILT N 
Sbjct: 250 PGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNG 309

Query: 318 AGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD-DKKNLSLV 377
           AGN+GP R T  S+APW+ +VAAS M+R F+T V LGN     G ++N+ D + K   LV
Sbjct: 310 AGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLV 369

Query: 378 YGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLMQGYSS 437
           YG    +    SS   +C    ++ K V+GKI++CD         + G  A ++   Y  
Sbjct: 370 YGKSASSRCDASSAG-FCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNPYED 429

Query: 438 SARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRGPNSLT 497
           +A  +  P S+L+  D   V  Y+ ST+ P A + KS TI +  AP V S+SSRGPN L 
Sbjct: 430 AASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLI 489

Query: 498 SDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVKTFH 557
            DILKPD++APG EILAA+ P  P +   D+R V Y +ISGTSMSCPHV  +AAY+KTFH
Sbjct: 490 HDILKPDITAPGSEILAAYSPYVPPS-ESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFH 549

Query: 558 PTWSPAAIKSALMTTASPMKEKI---NKEAEFAYGSGQVDPLKAIDPGLVYDAKESDYVK 617
           P WSP+ I+SA+MTTA PM       N+ AEFAYG+G VDP+ AI PGLVY+A +SD++ 
Sbjct: 550 PLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHIT 609

Query: 618 FLCGEGYNTTMVRRITGDNSACTSANIGRVW-DLNYPSFALSVARSQISNINQNFLRTVT 677
           FLCG  Y    +R I+GD+S+CT      +  +LNYPS +  V+ ++   +   F RTVT
Sbjct: 610 FLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKV--TFRRTVT 669

Query: 678 SVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGA--MTESRISASLVW 737
           +V    +T++A + G++ L + V P+VLS   + ++KSFT+TV GA    E+ +SA L+W
Sbjct: 670 NVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIW 729

BLAST of Tan0017682 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 588.2 bits (1515), Expect = 9.3e-168
Identity = 337/739 (45.60%), Postives = 459/739 (62.11%), Query Frame = 0

Query: 13  FNLVFLSLFSGLLA-SGFDSDDDGRKIYIVYMGS--KLKDPDSAHLHHRAMLEEVVGSNF 72
           F L+   L S +++ S    D   +++Y+VYMGS    +   +   HH ++L+EV G + 
Sbjct: 8   FCLISCVLVSFVISVSAVTDDSQDKQVYVVYMGSLPSSRLEYTPMSHHMSILQEVTGESS 67

Query: 73  APESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSFP--L 132
               ++ +YKRSFNGFA +L E E +++A  EGVVSVFP    +L TT SWDFL      
Sbjct: 68  VEGRLVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGK 127

Query: 133 YVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGAR 192
              R   +ES+TI+G +DSGIW +  SFSD+G+ PPP KWKG+C A  N +CN K+IGAR
Sbjct: 128 NTKRNLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFTCNNKLIGAR 187

Query: 193 AYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAVY 252
            Y +  +          D  GHGTHTAST AG+ V   S YG+G GTARGGVP++RIA Y
Sbjct: 188 DYTNEGTR---------DIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAY 247

Query: 253 KVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGILTS 312
           K C   GC+   +L+AFDDAIADGVD++S+SLG    + Y    I+IGAFHAM  GILT 
Sbjct: 248 KACSEMGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVKGILTV 307

Query: 313 NSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTADDKKNLSL 372
            SAGN GP   +  S+APWIL+VAAS  +R FVT V LGN     G ++N  D K     
Sbjct: 308 QSAGNGGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNYP 367

Query: 373 VYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLMQGYS 432
           +YGG                  S +  L+RGKILV +  + ++  ++       + + Y 
Sbjct: 368 LYGG------------------STDGPLLRGKILVSEDKVSSEIVVA------NINENYH 427

Query: 433 SSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRGPNSL 492
             A    LPSS L+  D  +V  Y+ ST+ P  T+ KS  I + +AP V  FSSRGPN++
Sbjct: 428 DYAYVSILPSSALSKDDFDSVISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTI 487

Query: 493 TSDILKPDLSAPGVEILAAWPPI-APITVPQDSRRVLYNIISGTSMSCPHVTAIAAYVKT 552
             DILKPD++APGVEILAA+ P+ +P    +D+R V Y+++SGTSMSCPHV  +AAY+KT
Sbjct: 488 AVDILKPDVTAPGVEILAAFSPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGVAAYIKT 547

Query: 553 FHPTWSPAAIKSALMTTASPMKEKINKEA--EFAYGSGQVDPLKAIDPGLVYDAKESDYV 612
           FHP WSP+ I+SA+MTTA PM       A  EFAYG+G VDP+ AI+PGLVY+  +SD++
Sbjct: 548 FHPEWSPSMIQSAIMTTAWPMNATGTAVASTEFAYGAGHVDPIAAINPGLVYEIGKSDHI 607

Query: 613 KFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVARSQISNINQNFLRTVT 672
            FLCG  YN T ++ I G+   CT   + R  +LNYPS +  + +S+ S I   F RTVT
Sbjct: 608 AFLCGLNYNATSLKLIAGEAVTCTGKTLPR--NLNYPSMSAKLPKSESSFI-VTFNRTVT 667

Query: 673 SVVSGKSTFRATI--SGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGAMTESRI--SASL 732
           +V +  ST+++ I  +    L + VSPSVLS   + +++SFT+TV G+  + ++  SA+L
Sbjct: 668 NVGTPNSTYKSKIVLNHGSNLKVEVSPSVLSMKSVKEKQSFTVTVSGSNIDPKLPSSANL 710

Query: 733 VWSNGLFKVRSPITVYTRS 740
           +WS+G   VRSPI VYT S
Sbjct: 728 IWSDGTHNVRSPIVVYTYS 710

BLAST of Tan0017682 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 587.8 bits (1514), Expect = 1.2e-167
Identity = 322/691 (46.60%), Postives = 448/691 (64.83%), Query Frame = 0

Query: 56  HHRAMLEEVVGSNFAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLH 115
           HH ++L+++VG+  A   ++ +YKRSFNGFA  L++ E+QK+   + VVSVFPS+ ++L 
Sbjct: 14  HHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELT 73

Query: 116 TTRSWDFLSFPLYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQAST 175
           TTRSWDF+ F     R +  ES+ IVGV+DSGIW +  SF D+G+ PPP KWKG C+   
Sbjct: 74  TTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGL 133

Query: 176 NISCNRKIIGARAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTA 235
             +CN K+IGAR Y   +        S  D  GHGTHTAST AG+ V  AS YGL  GTA
Sbjct: 134 KFACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTA 193

Query: 236 RGGVPSARIAVYKVCWSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIG 295
           RGGVPSARIA YKVC+ + C+D DILAAFDDAIADGVD++S+S+           S++IG
Sbjct: 194 RGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIG 253

Query: 296 AFHAMKHGILTSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIA 355
           +FHAM  GI+T+ SAGN+GP + +  +++PW+++VAAS  DR+F+  V LGN     GI+
Sbjct: 254 SFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGIS 313

Query: 356 INTAD-DKKNLSLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLS 415
           +NT + +     +VYG +V       + + YC    V+ +LV+GKI++CD  +  +    
Sbjct: 314 VNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL 373

Query: 416 FGEAAGVLMQG--YSSSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDAS 475
            G A GV++Q      SA   P P+S L   D  ++K Y+ S   P A + ++  I D  
Sbjct: 374 AG-AIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDRE 433

Query: 476 APAVISFSSRGPNSLTSDILKPDLSAPGVEILAAWPPIA---PITVPQDSRRVLYNIISG 535
           AP V SFSSRGP+ +  ++LKPD+SAPG+EILAA+ P+A       P+D R V Y+++SG
Sbjct: 434 APYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSG 493

Query: 536 TSMSCPHVTAIAAYVKTFHPTWSPAAIKSALMTTASPMKEKINKEAEFAYGSGQVDPLKA 595
           TSM+CPHV  +AAYVK+FHP WSP+AIKSA+MTTA+PM  K N E EFAYGSGQ++P KA
Sbjct: 494 TSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKA 553

Query: 596 IDPGLVYDAKESDYVKFLCGEGYNTTMVRRITGDNSACTSANIGRVWDLNYPSFALSVAR 655
            DPGLVY+ +  DY+K LC EG+++T +   +G N  C+      V DLNYP+    V  
Sbjct: 554 SDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFV-- 613

Query: 656 SQISNINQNFLRTVTSVVSGKSTFRATISGAQ-GLTITVSPSVLSFNGIGDQKSFTLTVQ 715
           S +   N  F RTVT+V    ST++A++   Q  L I++ P +L F  + ++KSF +T+ 
Sbjct: 614 SSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTIS 673

Query: 716 GAMTE--SRISASLVWSNGLFKVRSPITVYT 738
           G   +  S +S+S+VWS+G   VRSPI  Y+
Sbjct: 674 GKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

BLAST of Tan0017682 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 584.7 bits (1506), Expect = 1.0e-166
Identity = 338/739 (45.74%), Postives = 467/739 (63.19%), Query Frame = 0

Query: 10  SLIFNLVFLSLFSGLLASGFDSDDDGRKIYIVYMGSKLKDPDSAHL-HHRAMLEEVVGSN 69
           S IF L+ +S  S       D DD  ++ YIVYMG+     D   + HH ++L++V G +
Sbjct: 12  SCIFALLVVSFASA------DKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGES 71

Query: 70  FAPESVIYTYKRSFNGFAVKLNEEEAQKIATKEGVVSVFPSEENQLHTTRSWDFLSF--P 129
              + ++  YKRSFNGFA +L + E + +A+ + VVSVFP+++ +L TT SW+F+     
Sbjct: 72  SIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKES 131

Query: 130 LYVPRVNEVESNTIVGVLDSGIWSDHPSFSDQGYDPPPAKWKGICQASTNISCNRKIIGA 189
               R   +ES+TI+GV+DSGI+ +  SFS +G+ PPP KWKG+C+   N + N K+IGA
Sbjct: 132 KRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGA 191

Query: 190 RAYRSRSSLPPGDIRSPIDTNGHGTHTASTVAGSLVNKASMYGLGLGTARGGVPSARIAV 249
           R Y  +    P   R   D  GHG+HTAST AG+ V   S YGLG GTARGGVP+ARIAV
Sbjct: 192 RYYTPKLEGFPESAR---DYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAV 251

Query: 250 YKVC--WSDGCSDADILAAFDDAIADGVDILSVSLGGTRPQPYFLHSISIGAFHAMKHGI 309
           YKVC    DGC+   ILAAFDDAIAD VDI+++S+GG    P+    I+IGAFHAM  GI
Sbjct: 252 YKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGI 311

Query: 310 LTSNSAGNSGPGRSTTTSLAPWILSVAASTMDRKFVTPVQLGNTNIHQGIAINTAD-DKK 369
           L  NSAGNSGP  ST  S+APW+ +VAAS  +R FVT V LGN     G ++N+ D + K
Sbjct: 312 LIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKT-VGRSVNSFDLNGK 371

Query: 370 NLSLVYGGDVPNVGFRSSVSRYCEINSVNPKLVRGKILVCDAVMPAKAFLSFGEAAGVLM 429
              LVYG    +    +S   +C    ++ K V+GKI++CD+        + G  A ++ 
Sbjct: 372 KYPLVYGKSASSSCGAASAG-FCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVR 431

Query: 430 QGYSSSARSYPLPSSILNPTDGTNVKRYMTSTRFPTATLFKSTTIPDASAPAVISFSSRG 489
              +  A  +  P S+L   D   V  YM ST+ P A + KS TI +  AP V S+ SRG
Sbjct: 432 SHRTDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRG 491

Query: 490 PNSLTSDILKPDLSAPGVEILAAWPPIAPITVPQDSRRVLYNIISGTSMSCPHVTAIAAY 549
           PN++  DILKPD++APG EI+AA+ P AP ++  D+RRV Y++ +GTSMSCPHV  +AAY
Sbjct: 492 PNTIIPDILKPDITAPGSEIVAAYSPDAPPSI-SDTRRVKYSVDTGTSMSCPHVAGVAAY 551

Query: 550 VKTFHPTWSPAAIKSALMTTASPMK---EKINKEAEFAYGSGQVDPLKAIDPGLVYDAKE 609
           +K+FHP WSP+ I+SA+MTTA PM       N+ AEFAYG+G VDP+ AI PGLVY+A +
Sbjct: 552 LKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANK 611

Query: 610 SDYVKFLCGEGYNTTMVRRITGDNSACTSANIGRV-WDLNYPSFALSVARSQISNINQNF 669
           SD++ FLCG  Y    +R I+GD+S+CT      +  +LNYPS    V+ ++   +   F
Sbjct: 612 SDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKV--IF 671

Query: 670 LRTVTSVVSGKSTFRATISGAQGLTITVSPSVLSFNGIGDQKSFTLTVQGA--MTESRIS 729
            RTVT+V    +T++A + G++ L + V P+VLS   + ++KSFT+T  GA    E+ +S
Sbjct: 672 RRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVS 731

Query: 730 ASLVWSNGLFKVRSPITVY 737
           A L+WS+G+  VRSPI VY
Sbjct: 732 AQLIWSDGVHFVRSPIVVY 735