Tan0017256 (gene) Snake gourd v1

Overview


Name	Tan0017256
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Polcalcin Jun o
Location	LG11: 18549592 .. 18550527 (-)
RNA-Seq Expression	Tan0017256
Synteny	Tan0017256

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTGCATGACTACATTCAATTTTTCATGTGCAATCTCTTTTCTCTCTCTAAATTTGGAAAATCAAATTTCATGGGATTTCAATCTGTTACAAATTCCAATTCCTTCTCCGATTCTGTTTCTTCCAAATCCCGCATATATATCTCTAATTCCTTCCCGATTCATTATCTGTTTCCACTTCTCATTCTCTGATTGATCGATCGATTGATTGATTGATTGATTTTCTCCCCCTTTTTGTTCATCAATTTCGACGGAATTAAAACCGATCGAATCTCCCTGAGCTGTGAAATTATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCGGATACCTCTAGCGATTCACCACTTGGATCTACCACCGTTCCAATTCTCGGATCTCGAACTCCCTCTCGAGCGCAAATCGCGGAACTCGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGGGGAGCTTAGGCCAGCCGGCGACGGAGGAGGAGCTTCAGAACATGATCAAGGAGGTCGATGCCGACGGCGACGGCTACATCGATCTCGACGAGTTCATCGAACTCAACACCAAGGGCATCGATTCCAACGAGGCGCTGGAGAATCTGAAGGATGCATTCTCTGTCTACGACATCGACGGTAACGGATCCATCACGGCCGAGGAATTGCACGAGGTTTTGAAGAGTTTGGGCGACGATTGCTCGCTGGCGGATTGTCGGAAGATGATCACCGGCGTCGATAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGTCATGATGATGAGCGGTTCTCGATCGCAGAGCTTCAAAGGTTAATTTTTTTAATTTTTTTTATTTTTTGAAGAATTCAATGGTTAATTAATGCTAATTGATAATTATGAGTAATTAGCTATTTTGCTAA

mRNA sequence

CTTTGCATGACTACATTCAATTTTTCATGTGCAATCTCTTTTCTCTCTCTAAATTTGGAAAATCAAATTTCATGGGATTTCAATCTGTTACAAATTCCAATTCCTTCTCCGATTCTGTTTCTTCCAAATCCCGCATATATATCTCTAATTCCTTCCCGATTCATTATCTGTTTCCACTTCTCATTCTCTGATTGATCGATCGATTGATTGATTGATTGATTTTCTCCCCCTTTTTGTTCATCAATTTCGACGGAATTAAAACCGATCGAATCTCCCTGAGCTGTGAAATTATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCGGATACCTCTAGCGATTCACCACTTGGATCTACCACCGTTCCAATTCTCGGATCTCGAACTCCCTCTCGAGCGCAAATCGCGGAACTCGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGGGGAGCTTAGGCCAGCCGGCGACGGAGGAGGAGCTTCAGAACATGATCAAGGAGGTCGATGCCGACGGCGACGGCTACATCGATCTCGACGAGTTCATCGAACTCAACACCAAGGGCATCGATTCCAACGAGGCGCTGGAGAATCTGAAGGATGCATTCTCTGTCTACGACATCGACGGTAACGGATCCATCACGGCCGAGGAATTGCACGAGGTTTTGAAGAGTTTGGGCGACGATTGCTCGCTGGCGGATTGTCGGAAGATGATCACCGGCGTCGATAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGTCATGATGATGAGCGGTTCTCGATCGCAGAGCTTCAAAGGTTAATTTTTTTAATTTTTTTTATTTTTTGAAGAATTCAATGGTTAATTAATGCTAATTGATAATTATGAGTAATTAGCTATTTTGCTAA

Coding sequence (CDS)

ATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCGGATACCTCTAGCGATTCACCACTTGGATCTACCACCGTTCCAATTCTCGGATCTCGAACTCCCTCTCGAGCGCAAATCGCGGAACTCGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATTATGGGGAGCTTAGGCCAGCCGGCGACGGAGGAGGAGCTTCAGAACATGATCAAGGAGGTCGATGCCGACGGCGACGGCTACATCGATCTCGACGAGTTCATCGAACTCAACACCAAGGGCATCGATTCCAACGAGGCGCTGGAGAATCTGAAGGATGCATTCTCTGTCTACGACATCGACGGTAACGGATCCATCACGGCCGAGGAATTGCACGAGGTTTTGAAGAGTTTGGGCGACGATTGCTCGCTGGCGGATTGTCGGAAGATGATCACCGGCGTCGATAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGTCATGATGATGAGCGGTTCTCGATCGCAGAGCTTCAAAGGTTAA

Protein sequence

MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTPSRAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSRSQSFKG

Homology

[Close]

BLAST of Tan0017256 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.1e-56
Identity = 111/182 (60.99%), Postives = 137/182 (75.27%), Query Frame = 0


Query: 6   LFSRKKKSDTSSDSPLG-STTVPILGSRTPSRAQIAELEQVFKKFDVNGDGKICSSELGS 65
           +F++ + S+  S S +G     P L      + +I ELE VFKKFDVNGDGKI S ELG+
Sbjct: 1   MFNKNQGSNGGSSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGA 60

Query: 66  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKDAFSVYDI 125
           IM SLG    EEEL+  I E+D  GDGYI+ +EF+ELNTKG+D N+ LENLKDAFSVYDI
Sbjct: 61  IMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDI 120

Query: 126 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSRSQSF 185
           DGNGSI+AEELHEVL+SLGD+CS+A+CRKMI GVD++GDG I F+EFK+MM  GSR  + 
Sbjct: 121 DGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRRDNV 180

Query: 186 KG 187
            G
Sbjct: 181 MG 182

[Close]

BLAST of Tan0017256 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 5.8e-39
Identity = 88/175 (50.29%), Postives = 116/175 (66.29%), Query Frame = 0


Query: 5   SLFSRKKKSDTSSDSPLGSTTVPILGSRTPSRAQIAELEQVFKKFDVNGDGKICSSELGS 64
           +LF R+    + S SP      P  G  +P+     E+E+VF+KFD NGDG+I  SELG+
Sbjct: 8   ALFRRR----SGSKSPPLPQADPASGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGA 67

Query: 65  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELN-TKGIDSNEALENLKDAFSVYD 124
           +  SLG  AT++EL  M+ E DADGDG+I LDEF  LN T   D+    E+L+ AF V+D
Sbjct: 68  LFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFD 127

Query: 125 IDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSG 179
            DGNG+I+A EL  VL  LG+  ++  CR+MI GVD+NGDG+ISF+EFKVMM  G
Sbjct: 128 ADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGG 178

[Close]

BLAST of Tan0017256 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 1.9e-34
Identity = 73/145 (50.34%), Postives = 103/145 (71.03%), Query Frame = 0


Query: 39  IAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEF 98
           + ELE+VFKKFD NGDGKI  SEL  I+ SLG    E E++ M++E DADGDGY+ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 99  IELNTKGIDSNEALENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGV 158
           ++LN KG     ++++LK+AF V+D D NGSI+A EL   L+S+G+ C++ + + +I  V
Sbjct: 84  VDLNNKG----ASVKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 159 DRNGDGMISFDEFKVMMMSGSRSQS 184
           D+NGDG+IS +EF+ MM S    +S
Sbjct: 144 DKNGDGLISVEEFQTMMTSEMTDKS 164

[Close]

BLAST of Tan0017256 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 3.3e-34
Identity = 70/142 (49.30%), Postives = 104/142 (73.24%), Query Frame = 0


Query: 41  ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 100
           ++++VF++FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDLDEF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 101 LNTKGI----DSNEALENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMIT 160
           L   GI    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG+ CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 161 GVDRNGDGMISFDEFKVMMMSG 179
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of Tan0017256 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 9.6e-34
Identity = 68/138 (49.28%), Postives = 101/138 (73.19%), Query Frame = 0


Query: 41  ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 100
           ++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D DG+G+IDLDEF+ 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 101 L--NTKGIDSNEALENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGV 160
           L   +    +N A+ +LK+AF +YD+D NG I+A ELH V+K+LG+ CS+ DC++MI  V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 161 DRNGDGMISFDEFKVMMM 177
           D +GDG + F+EFK MMM
Sbjct: 135 DSDGDGCVDFEEFKKMMM 152

[Close]

BLAST of Tan0017256 vs. NCBI nr
Match: XP_038875473.1 (probable calcium-binding protein CML25 [Benincasa hispida])

HSP 1 Score: 339.0 bits (868), Expect = 2.7e-89
Identity = 174/186 (93.55%), Postives = 179/186 (96.24%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTPSRAQIAELEQVFKKFDVNGDGKICSS 60
           MGFKSLFSRKKKS +S+DSPLGSTTVPILGS   SRAQIAELEQVFKKFDVNGDGKICSS
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTVPILGSLN-SRAQIAELEQVFKKFDVNGDGKICSS 60

Query: 61  ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKDAFS 120
           ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKG+DS+E LENLKDAFS
Sbjct: 61  ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFS 120

Query: 121 VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 180
           VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVD+NGDGMISFDEFKVMMMSGSR
Sbjct: 121 VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 180

Query: 181 SQSFKG 187
           SQ F G
Sbjct: 181 SQGFNG 185

[Close]

BLAST of Tan0017256 vs. NCBI nr
Match: XP_011655449.1 (probable calcium-binding protein CML25 [Cucumis sativus] >KGN51467.1 hypothetical protein Csa_008232 [Cucumis sativus])

HSP 1 Score: 334.3 bits (856), Expect = 6.5e-88
Identity = 171/186 (91.94%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTPSRAQIAELEQVFKKFDVNGDGKICSS 60
           MGFKSLFSRKKKS +S+DSPLGSTT+PILGSRT    QIAELEQVF KFDVNGDGKICSS
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRT----QIAELEQVFNKFDVNGDGKICSS 60

Query: 61  ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKDAFS 120
           ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKG+DS+E LENLKDAFS
Sbjct: 61  ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFS 120

Query: 121 VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 180
           VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVD+NGDGMISFDEFKVMMMSGSR
Sbjct: 121 VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 180

Query: 181 SQSFKG 187
           SQ F G
Sbjct: 181 SQGFNG 182

[Close]

BLAST of Tan0017256 vs. NCBI nr
Match: XP_023513887.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 334.0 bits (855), Expect = 8.5e-88
Identity = 171/184 (92.93%), Postives = 177/184 (96.20%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTP---SRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDSPLGSTTVPILGSRTP   SRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKG+DS+E L+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVDRNGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRS 182
           GSRS
Sbjct: 181 GSRS 184

[Close]

BLAST of Tan0017256 vs. NCBI nr
Match: XP_022963580.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 334.0 bits (855), Expect = 8.5e-88
Identity = 171/184 (92.93%), Postives = 177/184 (96.20%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTP---SRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDSPLGSTTVPILGSRTP   SRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKG+DS+E L+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVDRNGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRS 182
           GSRS
Sbjct: 181 GSRS 184

[Close]

BLAST of Tan0017256 vs. NCBI nr
Match: XP_022990613.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 334.0 bits (855), Expect = 8.5e-88
Identity = 171/184 (92.93%), Postives = 177/184 (96.20%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTP---SRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDSPLGSTTVPILGSRTP   SRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKG+DS+E L+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVDRNGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRS 182
           GSRS
Sbjct: 181 GSRS 184

[Close]

BLAST of Tan0017256 vs. ExPASy TrEMBL
Match: A0A0A0KST2 (Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.2e-88
Identity = 171/186 (91.94%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTPSRAQIAELEQVFKKFDVNGDGKICSS 60
           MGFKSLFSRKKKS +S+DSPLGSTT+PILGSRT    QIAELEQVF KFDVNGDGKICSS
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRT----QIAELEQVFNKFDVNGDGKICSS 60

Query: 61  ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKDAFS 120
           ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKG+DS+E LENLKDAFS
Sbjct: 61  ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFS 120

Query: 121 VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 180
           VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVD+NGDGMISFDEFKVMMMSGSR
Sbjct: 121 VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 180

Query: 181 SQSFKG 187
           SQ F G
Sbjct: 181 SQGFNG 182

[Close]

BLAST of Tan0017256 vs. ExPASy TrEMBL
Match: A0A6J1JTS9 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487441 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.1e-88
Identity = 171/184 (92.93%), Postives = 177/184 (96.20%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTP---SRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDSPLGSTTVPILGSRTP   SRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKG+DS+E L+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVDRNGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRS 182
           GSRS
Sbjct: 181 GSRS 184

[Close]

BLAST of Tan0017256 vs. ExPASy TrEMBL
Match: A0A6J1HID9 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111463873 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.1e-88
Identity = 171/184 (92.93%), Postives = 177/184 (96.20%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTP---SRAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD+SSDSPLGSTTVPILGSRTP   SRAQIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKG+DS+E L+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVDRNGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

Query: 181 GSRS 182
           GSRS
Sbjct: 181 GSRS 184

[Close]

BLAST of Tan0017256 vs. ExPASy TrEMBL
Match: A0A6J1CZ20 (probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC111015568 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.2e-87
Identity = 171/189 (90.48%), Postives = 176/189 (93.12%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTPS---RAQIAELEQVFKKFDVNGDGKI 60
           MGFKSLFSRKKKSD SSDSP GST VP+LGSRTPS   R QIAELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKD 120
            SSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDLDEFIELNTKG+DS E LENLKD
Sbjct: 61  SSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180
           AFSVYDIDGNGSITAEELHEVLKSLGD+CSLADCRKMITGVDRNGDGMISFDEFKVMMMS
Sbjct: 121 AFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMS 180

Query: 181 GSRSQSFKG 187
           GSRS  F G
Sbjct: 181 GSRSHGFGG 189

[Close]

BLAST of Tan0017256 vs. ExPASy TrEMBL
Match: A0A5A7TS48 (Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3042G00010 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 6.0e-87
Identity = 169/186 (90.86%), Postives = 173/186 (93.01%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTSSDSPLGSTTVPILGSRTPSRAQIAELEQVFKKFDVNGDGKICSS 60
           MGFKSLFSRKKKS  S+DSPLGSTT+PI GSRT    QIAELEQVF KFDVNGDGKICSS
Sbjct: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRT----QIAELEQVFNKFDVNGDGKICSS 60

Query: 61  ELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKDAFS 120
           ELGSIMGSLGQPATEEEL NMIKEVDADGDGYIDLDEFIELNTKG+DSNE LENLKDAFS
Sbjct: 61  ELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFS 120

Query: 121 VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 180
           VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVD+NGDGMISFDEFKVMMMSGSR
Sbjct: 121 VYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 180

Query: 181 SQSFKG 187
           S  F G
Sbjct: 181 SHGFNG 182

[Close]

BLAST of Tan0017256 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 221.1 bits (562), Expect = 7.5e-58
Identity = 111/182 (60.99%), Postives = 137/182 (75.27%), Query Frame = 0


Query: 6   LFSRKKKSDTSSDSPLG-STTVPILGSRTPSRAQIAELEQVFKKFDVNGDGKICSSELGS 65
           +F++ + S+  S S +G     P L      + +I ELE VFKKFDVNGDGKI S ELG+
Sbjct: 1   MFNKNQGSNGGSSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGA 60

Query: 66  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGIDSNEALENLKDAFSVYDI 125
           IM SLG    EEEL+  I E+D  GDGYI+ +EF+ELNTKG+D N+ LENLKDAFSVYDI
Sbjct: 61  IMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDI 120

Query: 126 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSRSQSF 185
           DGNGSI+AEELHEVL+SLGD+CS+A+CRKMI GVD++GDG I F+EFK+MM  GSR  + 
Sbjct: 121 DGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRRDNV 180

Query: 186 KG 187
            G
Sbjct: 181 MG 182

[Close]

BLAST of Tan0017256 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 146.4 bits (368), Expect = 2.4e-35
Identity = 70/142 (49.30%), Postives = 104/142 (73.24%), Query Frame = 0


Query: 41  ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 100
           ++++VF++FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDLDEF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 101 LNTKGI----DSNEALENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMIT 160
           L   GI    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG+ CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 161 GVDRNGDGMISFDEFKVMMMSG 179
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of Tan0017256 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 144.8 bits (364), Expect = 6.8e-35
Identity = 68/138 (49.28%), Postives = 101/138 (73.19%), Query Frame = 0


Query: 41  ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 100
           ++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D DG+G+IDLDEF+ 
Sbjct: 15  DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVA 74

Query: 101 L--NTKGIDSNEALENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGV 160
           L   +    +N A+ +LK+AF +YD+D NG I+A ELH V+K+LG+ CS+ DC++MI  V
Sbjct: 75  LFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINKV 134

Query: 161 DRNGDGMISFDEFKVMMM 177
           D +GDG + F+EFK MMM
Sbjct: 135 DSDGDGCVDFEEFKKMMM 152

[Close]

BLAST of Tan0017256 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.8 bits (338), Expect = 7.1e-32
Identity = 73/156 (46.79%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 27  PILGSRTPSRAQIA---ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIK 86
           P     TP+   +A   EL++VF +FD NGDGKI   ELG +  ++G   TE EL  +++
Sbjct: 6   PETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLE 65

Query: 87  EVDADGDGYIDLDEFIELNTKGIDSNEALENLKDAFSVYDIDGNGSITAEELHEVLKSLG 146
           EVD D DGYI+LDEF  L      S+ +   ++DAF +YD D NG I+A ELH+VL  LG
Sbjct: 66  EVDTDRDGYINLDEFSTL----CRSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLG 125

Query: 147 DDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSGS 180
             CS+ DC +MI  VD +GDG ++F+EF+ MM S S
Sbjct: 126 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

[Close]

BLAST of Tan0017256 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.8 bits (338), Expect = 7.1e-32
Identity = 73/156 (46.79%), Postives = 99/156 (63.46%), Query Frame = 0


Query: 27  PILGSRTPSRAQIA---ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIK 86
           P     TP+   +A   EL++VF +FD NGDGKI   ELG +  ++G   TE EL  +++
Sbjct: 6   PETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLE 65

Query: 87  EVDADGDGYIDLDEFIELNTKGIDSNEALENLKDAFSVYDIDGNGSITAEELHEVLKSLG 146
           EVD D DGYI+LDEF  L      S+ +   ++DAF +YD D NG I+A ELH+VL  LG
Sbjct: 66  EVDTDRDGYINLDEFSTL----CRSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLG 125

Query: 147 DDCSLADCRKMITGVDRNGDGMISFDEFKVMMMSGS 180
             CS+ DC +MI  VD +GDG ++F+EF+ MM S S
Sbjct: 126 MSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

The following BLAST results are available for this feature:

BLAST of Tan0017256 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYK2	1.1e-56	60.99	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q8RZB5	5.8e-39	50.29	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
O64943	1.9e-34	50.34	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
P25070	3.3e-34	49.30	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q9C8Y1	9.6e-34	49.28	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of Tan0017256 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875473.1	2.7e-89	93.55	probable calcium-binding protein CML25 [Benincasa hispida]	[more]
XP_011655449.1	6.5e-88	91.94	probable calcium-binding protein CML25 [Cucumis sativus] >KGN51467.1 hypothetica...	[more]
XP_023513887.1	8.5e-88	92.93	probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo]	[more]
XP_022963580.1	8.5e-88	92.93	probable calcium-binding protein CML25 [Cucurbita moschata]	[more]
XP_022990613.1	8.5e-88	92.93	probable calcium-binding protein CML25 [Cucurbita maxima]	[more]

BLAST of Tan0017256 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KST2	3.2e-88	91.94	Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1	[more]
A0A6J1JTS9	4.1e-88	92.93	probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114874...	[more]
A0A6J1HID9	4.1e-88	92.93	probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1CZ20	1.2e-87	90.48	probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5A7TS48	6.0e-87	90.86	Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Tan0017256 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	7.5e-58	60.99	EF hand calcium-binding protein family	[more]
AT5G37770.1	2.4e-35	49.30	EF hand calcium-binding protein family	[more]
AT1G66400.1	6.8e-35	49.28	calmodulin like 23	[more]
AT1G18210.1	7.1e-32	46.79	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	7.1e-32	46.79	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 77..105 e-value: 4.2E-5 score: 32.9 coord: 114..142 e-value: 1.3E-5 score: 34.7 coord: 150..178 e-value: 2.9E-5 score: 33.5 coord: 41..69 e-value: 4.4E-6 score: 36.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 41..100 e-value: 3.2E-14 score: 53.1 coord: 113..175 e-value: 4.7E-12 score: 46.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 37..72 score: 15.071314
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 146..181 score: 12.281773
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 110..145 score: 15.685013
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 73..108 score: 13.899706
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 41..101 e-value: 3.37845E-20 score: 77.9733
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 115..176 e-value: 2.03248E-19 score: 75.6621
None	No IPR available	GENE3D	1.10.238.10		coord: 8..104 e-value: 1.4E-25 score: 91.5 coord: 105..183 e-value: 2.4E-25 score: 90.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..32
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..32
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 32..184
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 32..184
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 123..135
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 86..98
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 159..171
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 50..62
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 32..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0017256.1	Tan0017256.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding