Tan0016493 (gene) Snake gourd v1

Overview


Name	Tan0016493
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	cysteine proteinase inhibitor 5-like
Location	LG05: 63308009 .. 63308857 (+)
RNA-Seq Expression	Tan0016493
Synteny	Tan0016493

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAGCCGTTCCAATCTCGTTCACTCCTTTTCGAGCGGACCGGTTTCCGCGTCGAAATGAACTCCTATAAATTCCCCTAAAACCACTCACCTAAGCAATCGCCTCAAAAATTTCCCTCAATTCATTCATTCTCTTACTCGCCCGACGGTTACAATGGAATCCCGCTCCGCTTCTCTCTTCCTCATCCTTCTCCTCCCGCTCCTGGTCGCCACCATGACCGCCGCCACCGGTCGGAAGGGGAATCTGGTCGGCGGCTGGAAGCCGATTGCAGACGTGAAGGATCCGCACGTTCAAGAGATCGGAAAGTTCGCAGTGACAGAGCAGAACAGGAAATCCCAAAGCAAACTCGTTTTCCAGGACGTCCTCAGCGCCGAATCGCAGGTCGTCTCCGGTACCAATTACCGCATGGTCATCTTGGTTAAGGACGGCGGTTCGGATGCCAAATATGAGGCTAACATCTGGGAGAAGCCATGGGAGCATTTCAAGCAACTCACGTCCTTCAAGCACCTCGCCTAATTCCACCAGTAAAGCCCTAATTTAATTTCCCTTTTTTCTCGTCCGACGATCGTGATGTTTGTGTGGCTCTTCTGGCTGTTTTCTGTTTGCTGTTTGCTATTAGTATTTGGAGATTAGAAAATAAAATTTCGTAGCGATTTCTGGAGAAATCGTTTGTCGATTTTGTTCTTGTTATCAGATCTCTGTTTTCTTGAACAATCTTATTTGTGTTAGTTTGAAGGCCCTTTGTAATGAATGTTATATTAAAAAGGAAAAGTTATTAATATCTCAAATGCTATTGCTGCTTCCTACCTGTATAAAAGTGATCGAATAAATTATCCAACCTTTAATTTT

mRNA sequence

GAAAGCCGTTCCAATCTCGTTCACTCCTTTTCGAGCGGACCGGTTTCCGCGTCGAAATGAACTCCTATAAATTCCCCTAAAACCACTCACCTAAGCAATCGCCTCAAAAATTTCCCTCAATTCATTCATTCTCTTACTCGCCCGACGGTTACAATGGAATCCCGCTCCGCTTCTCTCTTCCTCATCCTTCTCCTCCCGCTCCTGGTCGCCACCATGACCGCCGCCACCGGTCGGAAGGGGAATCTGGTCGGCGGCTGGAAGCCGATTGCAGACGTGAAGGATCCGCACGTTCAAGAGATCGGAAAGTTCGCAGTGACAGAGCAGAACAGGAAATCCCAAAGCAAACTCGTTTTCCAGGACGTCCTCAGCGCCGAATCGCAGGTCGTCTCCGGTACCAATTACCGCATGGTCATCTTGGTTAAGGACGGCGGTTCGGATGCCAAATATGAGGCTAACATCTGGGAGAAGCCATGGGAGCATTTCAAGCAACTCACGTCCTTCAAGCACCTCGCCTAATTCCACCAGTAAAGCCCTAATTTAATTTCCCTTTTTTCTCGTCCGACGATCGTGATGTTTGTGTGGCTCTTCTGGCTGTTTTCTGTTTGCTGTTTGCTATTAGTATTTGGAGATTAGAAAATAAAATTTCGTAGCGATTTCTGGAGAAATCGTTTGTCGATTTTGTTCTTGTTATCAGATCTCTGTTTTCTTGAACAATCTTATTTGTGTTAGTTTGAAGGCCCTTTGTAATGAATGTTATATTAAAAAGGAAAAGTTATTAATATCTCAAATGCTATTGCTGCTTCCTACCTGTATAAAAGTGATCGAATAAATTATCCAACCTTTAATTTT

Coding sequence (CDS)

ATGGAATCCCGCTCCGCTTCTCTCTTCCTCATCCTTCTCCTCCCGCTCCTGGTCGCCACCATGACCGCCGCCACCGGTCGGAAGGGGAATCTGGTCGGCGGCTGGAAGCCGATTGCAGACGTGAAGGATCCGCACGTTCAAGAGATCGGAAAGTTCGCAGTGACAGAGCAGAACAGGAAATCCCAAAGCAAACTCGTTTTCCAGGACGTCCTCAGCGCCGAATCGCAGGTCGTCTCCGGTACCAATTACCGCATGGTCATCTTGGTTAAGGACGGCGGTTCGGATGCCAAATATGAGGCTAACATCTGGGAGAAGCCATGGGAGCATTTCAAGCAACTCACGTCCTTCAAGCACCTCGCCTAA

Protein sequence

MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRKSQSKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFKHLA

Homology

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BLAST of Tan0016493 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 3.8e-23
Identity = 53/117 (45.30%), Postives = 76/117 (64.96%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M  +  SL L+LLL L  A +    GRK    GGW+PI ++    VQ++ +FAV+E N++
Sbjct: 1   MVPKPLSLLLLLLLALSAAVV---GGRKRVAAGGWRPIENLNSAEVQDVAQFAVSEHNKQ 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFK 118
           +  +L +Q V+   +QVV+GTNYR+VI  KDG     YEA +W+KPW HF+ LTSF+
Sbjct: 61  ANDELQYQSVVRGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTSFR 114

[Close]

BLAST of Tan0016493 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.4e-22
Identity = 53/117 (45.30%), Postives = 74/117 (63.25%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M  +  SL L LLL L  A +    GRK    GGW+PI  +    VQ++ +FAV+E N++
Sbjct: 1   MVPKPLSLLLFLLLALSAAVV---GGRKLVAAGGWRPIESLNSAEVQDVAQFAVSEHNKQ 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFK 118
           +  +L +Q V+   +QVV+GTNYR+VI  KDG     YEA +W+KPW HF+ LTSF+
Sbjct: 61  ANDELQYQSVVRGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTSFR 114

[Close]

BLAST of Tan0016493 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 106.7 bits (265), Expect = 1.9e-22
Identity = 56/118 (47.46%), Postives = 78/118 (66.10%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M S+   L L+ L+ +L+    +A  R    VGGW PI++V DP V EIG+FAV+E N++
Sbjct: 1   MTSKVVFLLLLSLVVVLLPLYASAAAR----VGGWSPISNVTDPQVVEIGEFAVSEYNKR 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDG-GSDAKYEANIWEKPWEHFKQLTSFK 118
           S+S L F+ V+S E+QVVSGTNYR+ +   DG G    Y A +W+KPW  F+ LTSF+
Sbjct: 61  SESGLKFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFE 114

[Close]

BLAST of Tan0016493 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 4.3e-19
Identity = 54/116 (46.55%), Postives = 69/116 (59.48%), Query Frame = 0


Query: 8   LFLILLLPLLVATMTAATGRKGN--LVGGWKPIADVKDPHVQEIGKFAVTEQNRKSQSKL 67
           L L LLL +  A      G +G+  LVGGW PI DV DPH+QE+G +AV      S   L
Sbjct: 6   LLLALLLAVSAAAAAQVGGNRGHGPLVGGWSPITDVGDPHIQELGGWAVERHASLSSDGL 65

Query: 68  VFQDVLSAESQVVSGTNYRMVILVKD-GGSDAKYEANIWEKPWEHFKQLTSFKHLA 121
            F+ V S E QVVSG NYR+V+   D  G+ A Y A ++E+ W + +QLTSFK  A
Sbjct: 66  RFRRVTSGEQQVVSGMNYRLVVSASDPAGATASYVAVVYEQSWTNTRQLTSFKPAA 121

[Close]

BLAST of Tan0016493 vs. ExPASy Swiss-Prot
Match: P31726 (Cystatin-1 OS=Zea mays OX=4577 GN=RAMDAZC7 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 6.2e-18
Identity = 50/115 (43.48%), Postives = 73/115 (63.48%), Query Frame = 0


Query: 10  LILLLPLLVATMTAATGRK------GNLVGGWKPI-ADVKDPHVQEIGKFAVTEQNRKSQ 69
           L++LL L   + T +T ++      G L GG K + A+  D  +QE+ +FAV E N+K+ 
Sbjct: 13  LLVLLALAAVSSTRSTQKESVADNAGMLAGGIKDVPANENDLQLQELARFAVNEHNQKAN 72

Query: 70  SKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFK 118
           + L F+ ++ A++QVV+GT Y + I VKDG     YEA +WEKPWE+FKQL  FK
Sbjct: 73  ALLGFEKLVKAKTQVVAGTMYYLTIEVKDGEVKKLYEAKVWEKPWENFKQLQEFK 127

[Close]

BLAST of Tan0016493 vs. NCBI nr
Match: XP_023520814.1 (cysteine proteinase inhibitor 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 155.6 bits (392), Expect = 2.7e-34
Identity = 79/120 (65.83%), Postives = 94/120 (78.33%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M+SRSASLFLILLLPLLV     AT R G LVGGW+ I DVKDPH+QEIGKFAV+E N++
Sbjct: 1   MKSRSASLFLILLLPLLV----VATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQ 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFKHLA 120
           S+  L F+DV+  ESQVVSGTNYR+VI  K G S  KY+A +WEK W+HF +LTSFK +A
Sbjct: 61  SKGALEFKDVVKGESQVVSGTNYRLVIEAKKGESIGKYQALVWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Tan0016493 vs. NCBI nr
Match: XP_022927193.1 (cysteine proteinase inhibitor 5-like [Cucurbita moschata])

HSP 1 Score: 153.7 bits (387), Expect = 1.0e-33
Identity = 79/120 (65.83%), Postives = 93/120 (77.50%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M+SRSASLFLILLLPLLV     AT R G LVGGW+ I DVKDPH+QEIGKFAV+E N++
Sbjct: 1   MKSRSASLFLILLLPLLV----VATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQ 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFKHLA 120
           S+  L F+DV+  ESQVVSG NYR+VI  K G S  KY+A IWEK W+HF +LTSFK +A
Sbjct: 61  SKGALEFKDVVKGESQVVSGMNYRLVIEAKKGESIGKYQALIWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Tan0016493 vs. NCBI nr
Match: XP_022973098.1 (cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine proteinase inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 153.3 bits (386), Expect = 1.3e-33
Identity = 78/120 (65.00%), Postives = 93/120 (77.50%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M+SRSASLFLILLLPLLV     AT R G LVGGW+ I DVKDPH+QEIGKFAV+E N++
Sbjct: 1   MKSRSASLFLILLLPLLV----VATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQ 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFKHLA 120
           S+  L F+DV+  ESQVVSG NYR+VI  K G S  KY+A +WEK W+HF +LTSFK +A
Sbjct: 61  SKGALEFKDVVKGESQVVSGMNYRLVIEAKKGESIGKYQALVWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Tan0016493 vs. NCBI nr
Match: XP_022142402.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 152.1 bits (383), Expect = 3.0e-33
Identity = 78/121 (64.46%), Postives = 94/121 (77.69%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M+SRSA L L+LLLP +VA + A   RKG LVGGW PI DVKDPHVQEIGKFAV   N +
Sbjct: 7   MKSRSAPLLLLLLLPAMVAAV-AVAARKGPLVGGWTPITDVKDPHVQEIGKFAVAAYNTQ 66

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKD-GGSDAKYEANIWEKPWEHFKQLTSFKHL 120
           S+  + F  V S E+QVVSGTNYR+V++ KD GGS AKY+A +WEKPWE+FK+LTSFK +
Sbjct: 67  SKGAIKFDRVESGETQVVSGTNYRLVVVAKDGGGSTAKYQAIVWEKPWENFKKLTSFKPV 126

[Close]

BLAST of Tan0016493 vs. NCBI nr
Match: XP_006449877.1 (cysteine proteinase inhibitor 1 [Citrus clementina] >ESR63117.1 hypothetical protein CICLE_v10017209mg [Citrus clementina] >GAY35351.1 hypothetical protein CUMW_015810 [Citrus unshiu])

HSP 1 Score: 139.4 bits (350), Expect = 2.0e-29
Identity = 67/110 (60.91%), Postives = 84/110 (76.36%), Query Frame = 0


Query: 8   LFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRKSQSKLVF 67
           L  +++L L V  + AA  RKG LVGGWKPI D K+ HV EIG+FAVTE N++S+S L F
Sbjct: 5   LCCLIVLFLSVVPLLAAGDRKGTLVGGWKPIEDPKEKHVMEIGQFAVTEYNKRSKSALKF 64

Query: 68  QDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFK 118
           + V   E+QVVSGTNYR++++VKDG S  K+EA +WEKPWEHFK LTSFK
Sbjct: 65  ESVEKGETQVVSGTNYRLILVVKDGPSTKKFEAVVWEKPWEHFKSLTSFK 114

[Close]

BLAST of Tan0016493 vs. ExPASy TrEMBL
Match: A0A6J1EKC1 (cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111434113 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.9e-34
Identity = 79/120 (65.83%), Postives = 93/120 (77.50%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M+SRSASLFLILLLPLLV     AT R G LVGGW+ I DVKDPH+QEIGKFAV+E N++
Sbjct: 1   MKSRSASLFLILLLPLLV----VATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQ 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFKHLA 120
           S+  L F+DV+  ESQVVSG NYR+VI  K G S  KY+A IWEK W+HF +LTSFK +A
Sbjct: 61  SKGALEFKDVVKGESQVVSGMNYRLVIEAKKGESIGKYQALIWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Tan0016493 vs. ExPASy TrEMBL
Match: A0A6J1IC35 (cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 6.4e-34
Identity = 78/120 (65.00%), Postives = 93/120 (77.50%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M+SRSASLFLILLLPLLV     AT R G LVGGW+ I DVKDPH+QEIGKFAV+E N++
Sbjct: 1   MKSRSASLFLILLLPLLV----VATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQ 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFKHLA 120
           S+  L F+DV+  ESQVVSG NYR+VI  K G S  KY+A +WEK W+HF +LTSFK +A
Sbjct: 61  SKGALEFKDVVKGESQVVSGMNYRLVIEAKKGESIGKYQALVWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Tan0016493 vs. ExPASy TrEMBL
Match: A0A6J1CMN4 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111012543 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.4e-33
Identity = 78/121 (64.46%), Postives = 94/121 (77.69%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M+SRSA L L+LLLP +VA + A   RKG LVGGW PI DVKDPHVQEIGKFAV   N +
Sbjct: 7   MKSRSAPLLLLLLLPAMVAAV-AVAARKGPLVGGWTPITDVKDPHVQEIGKFAVAAYNTQ 66

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKD-GGSDAKYEANIWEKPWEHFKQLTSFKHL 120
           S+  + F  V S E+QVVSGTNYR+V++ KD GGS AKY+A +WEKPWE+FK+LTSFK +
Sbjct: 67  SKGAIKFDRVESGETQVVSGTNYRLVVVAKDGGGSTAKYQAIVWEKPWENFKKLTSFKPV 126

[Close]

BLAST of Tan0016493 vs. ExPASy TrEMBL
Match: V4UAT4 (Cystatin domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10017209mg PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 9.6e-30
Identity = 67/110 (60.91%), Postives = 84/110 (76.36%), Query Frame = 0


Query: 8   LFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRKSQSKLVF 67
           L  +++L L V  + AA  RKG LVGGWKPI D K+ HV EIG+FAVTE N++S+S L F
Sbjct: 5   LCCLIVLFLSVVPLLAAGDRKGTLVGGWKPIEDPKEKHVMEIGQFAVTEYNKRSKSALKF 64

Query: 68  QDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFK 118
           + V   E+QVVSGTNYR++++VKDG S  K+EA +WEKPWEHFK LTSFK
Sbjct: 65  ESVEKGETQVVSGTNYRLILVVKDGPSTKKFEAVVWEKPWEHFKSLTSFK 114

[Close]

BLAST of Tan0016493 vs. ExPASy TrEMBL
Match: A0A2H5N6E4 (Cystatin domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_015810 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 9.6e-30
Identity = 67/110 (60.91%), Postives = 84/110 (76.36%), Query Frame = 0


Query: 8   LFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRKSQSKLVF 67
           L  +++L L V  + AA  RKG LVGGWKPI D K+ HV EIG+FAVTE N++S+S L F
Sbjct: 5   LCCLIVLFLSVVPLLAAGDRKGTLVGGWKPIEDPKEKHVMEIGQFAVTEYNKRSKSALKF 64

Query: 68  QDVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFK 118
           + V   E+QVVSGTNYR++++VKDG S  K+EA +WEKPWEHFK LTSFK
Sbjct: 65  ESVEKGETQVVSGTNYRLILVVKDGPSTKKFEAVVWEKPWEHFKSLTSFK 114

[Close]

BLAST of Tan0016493 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 106.7 bits (265), Expect = 1.3e-23
Identity = 56/118 (47.46%), Postives = 78/118 (66.10%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M S+   L L+ L+ +L+    +A  R    VGGW PI++V DP V EIG+FAV+E N++
Sbjct: 1   MTSKVVFLLLLSLVVVLLPLYASAAAR----VGGWSPISNVTDPQVVEIGEFAVSEYNKR 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDG-GSDAKYEANIWEKPWEHFKQLTSFK 118
           S+S L F+ V+S E+QVVSGTNYR+ +   DG G    Y A +W+KPW  F+ LTSF+
Sbjct: 61  SESGLKFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFE 114

[Close]

BLAST of Tan0016493 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 1.9e-17
Identity = 53/120 (44.17%), Postives = 70/120 (58.33%), Query Frame = 0


Query: 1   MESRSASLFLILLLPLLVATMTAATGRKGNLVGGWKPIADVKDPHVQEIGKFAVTEQNRK 60
           M  +S     ++LLPL+  ++    G  G L G  KPI +V DP V  + K+A+ E N++
Sbjct: 1   MMMKSLICLSLILLPLV--SVVEGLGGGGGL-GSRKPIKNVSDPDVVAVAKYAIEEHNKE 60

Query: 61  SQSKLVFQDVLSAESQVVSGTNYRMVILVKDGGSDAK-YEANIWEKPWEHFKQLTSFKHL 120
           S+ KLVF  V+   +QVVSGT Y + I  KDGG   K YEA + EK W H K L SFK L
Sbjct: 61  SKEKLVFVKVVEGTTQVVSGTKYDLKIAAKDGGGKIKNYEAVVVEKLWLHSKSLESFKAL 117

[Close]

BLAST of Tan0016493 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 77.8 bits (190), Expect = 6.6e-15
Identity = 39/97 (40.21%), Postives = 60/97 (61.86%), Query Frame = 0


Query: 24  ATGRKGNLVGGWKPI-ADVKDPHVQEIGKFAVTEQNRKSQSKLVFQDVLSAESQVVSGTN 83
           A  + G +VGG + I A+  D  V+ + +FAV E N+     L ++ +L A++QVV+GT 
Sbjct: 2   ADQQAGTIVGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTM 61

Query: 84  YRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFKHL 120
           + + + V DG ++  YEA + EK WE+ KQL SF HL
Sbjct: 62  HHLTVEVADGETNKVYEAKVLEKAWENLKQLESFNHL 98

[Close]

BLAST of Tan0016493 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 70.1 bits (170), Expect = 1.4e-12
Identity = 38/109 (34.86%), Postives = 60/109 (55.05%), Query Frame = 0


Query: 10  LILLLPLLVATMTAATGRKGNLVGGWKPI-ADVKDPHVQEIGKFAVTEQNRKSQSKLVFQ 69
           L L +  L+A+       +  LVGG   + A+     V+ + +FAV E N+K  + L F 
Sbjct: 15  LSLFISSLIASDLGFCNEEMALVGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFA 74

Query: 70  DVLSAESQVVSGTNYRMVILVKDGGSDAKYEANIWEKPWEHFKQLTSFK 118
            V+ A+ QVV+GT + + + + + G    YEA +W KPW +FK+L  FK
Sbjct: 75  RVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEFK 123

[Close]

BLAST of Tan0016493 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 68.9 bits (167), Expect = 3.1e-12
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 0


Query: 31  LVGGWKPI-ADVKDPHVQEIGKFAVTEQNRKSQSKLVFQDVLSAESQVVSGTNYRMVILV 90
           LVGG   + A+     V+ + +FAV E N+K  + L F  V+ A+ QVV+GT + + + +
Sbjct: 3   LVGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEI 62

Query: 91  KDGGSDAKYEANIWEKPWEHFKQLTSFK 118
            + G    YEA +W KPW +FK+L  FK
Sbjct: 63  LEAGQKKLYEAKVWVKPWLNFKELQEFK 90

The following BLAST results are available for this feature:

BLAST of Tan0016493 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P86472	3.8e-23	45.30	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q6TPK4	1.4e-22	45.30	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
Q41916	1.9e-22	47.46	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
Q10J94	4.3e-19	46.55	Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]
P31726	6.2e-18	43.48	Cystatin-1 OS=Zea mays OX=4577 GN=RAMDAZC7 PE=2 SV=1	[more]

BLAST of Tan0016493 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520814.1	2.7e-34	65.83	cysteine proteinase inhibitor 5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927193.1	1.0e-33	65.83	cysteine proteinase inhibitor 5-like [Cucurbita moschata]	[more]
XP_022973098.1	1.3e-33	65.00	cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine p...	[more]
XP_022142402.1	3.0e-33	64.46	cysteine proteinase inhibitor 5-like [Momordica charantia]	[more]
XP_006449877.1	2.0e-29	60.91	cysteine proteinase inhibitor 1 [Citrus clementina] >ESR63117.1 hypothetical pro...	[more]

BLAST of Tan0016493 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EKC1	4.9e-34	65.83	cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC1114341...	[more]
A0A6J1IC35	6.4e-34	65.00	cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628...	[more]
A0A6J1CMN4	1.4e-33	64.46	cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
V4UAT4	9.6e-30	60.91	Cystatin domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10017...	[more]
A0A2H5N6E4	9.6e-30	60.91	Cystatin domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_015810 PE=4...	[more]

BLAST of Tan0016493 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	1.3e-23	47.46	Cystatin/monellin superfamily protein	[more]
AT4G16500.1	1.9e-17	44.17	Cystatin/monellin superfamily protein	[more]
AT5G12140.1	6.6e-15	40.21	cystatin-1	[more]
AT3G12490.2	1.4e-12	34.86	cystatin B	[more]
AT3G12490.1	3.1e-12	38.64	cystatin B	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 30..119 e-value: 2.8E-21 score: 86.7
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 38..118 e-value: 5.8E-33 score: 113.1
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 33..118 e-value: 5.32515E-19 score: 74.0476
None	No IPR available	GENE3D	3.10.450.10		coord: 18..120 e-value: 1.5E-36 score: 127.4
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 3..119
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 3..119
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 29..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0016493.1	Tan0016493.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity