Tan0016264 (gene) Snake gourd v1

BLAST of Tan0016264 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 551/759 (72.60%), Postives = 651/759 (85.77%), Query Frame = 0

Query: 12  LLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGGEDR 71
           L+F  +   +     KTYV+ MD+SAMP  +++H QWYS+ ++SV  +   E   G  +R
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE-EEGNNNR 79

Query: 72  IIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRFLGLEPADSNSA 131
           I+Y+YQ  FHG+AA+L++EEAERLEE+DGV+A+ PET+YELHTTRSP FLGLE  +S   
Sbjct: 80  ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERV 139

Query: 132 WSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGA 191
           W++++ DHDV+VGVLDTGIWPESESFND GMSPVP  W+G CETG+ F K+NCNRKIVGA
Sbjct: 140 WAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGA 199

Query: 192 RMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAP 251
           R+FY GYEAATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA 
Sbjct: 200 RVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQ 259

Query: 252 GARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAME 311
            AR+AAYKVCWVGGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAME
Sbjct: 260 KARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAME 319

Query: 312 MGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRI 371
           MGVFVSCSAGNGGPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR 
Sbjct: 320 MGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRT 379

Query: 372 TIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNA 431
            +P+NKQ+P+VY+G N+S+PDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK A
Sbjct: 380 VLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRA 439

Query: 432 GGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATATLGFLGTRLGV 491
           GGIGM+L+NTA NGEELVAD H++PAVA+GEKEGK IKQYA+T++KATA+L  LGTR+G+
Sbjct: 440 GGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGI 499

Query: 492 KPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSG 551
           KPSPVVAAFSSRGPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSG
Sbjct: 500 KPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSG 559

Query: 552 TSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHG 611
           TSMSCPHVSGVAALIKS+HPDW P+AIKSALMTTAYVHDN +KPL DAS A+PSSPYDHG
Sbjct: 560 TSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHG 619

Query: 612 AGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSC-HRLLPNPGDLN 671
           AGHI+P +A DPGLVY+I PQ+YFEFLCTQDL+P+QLKVF+K+SNR+C H L  NPG+LN
Sbjct: 620 AGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLN 679

Query: 672 YPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPENLNFTRSYQK 731
           YPAISA+FPE T V ++TL RTVTNVG   SSY   V+PF GA+V V+P+ LNFT  +QK
Sbjct: 680 YPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQK 739

Query: 732 LSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWL 770
           LSY +TF ++ R   PEFGGL+WK  TH+VRSP++ITWL
Sbjct: 740 LSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Tan0016264 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 9.9e-234
Identity = 405/743 (54.51%), Postives = 520/743 (69.99%), Query Frame = 0

Query: 28  TYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGGEDRIIYSYQNVFHGVAARL 87
           TY+V M +S MP SF  H  WY + L S+  + E          ++Y+Y+N  HG + RL
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE----------LLYTYENAIHGFSTRL 90

Query: 88  SEEEAERLEEQDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           ++EEA+ L  Q GV+++ PE +YELHTTR+P FLGL+   ++  + +  +  DV+VGVLD
Sbjct: 91  TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD-LFPEAGSYSDVVVGVLD 150

Query: 148 TGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGARMFYHGYEAATGKFNE 207
           TG+WPES+S++D G  P+P  WKG CE G  FT   CNRK++GAR F  GYE+  G  +E
Sbjct: 151 TGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDE 210

Query: 208 KLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCF 267
             E +SPRD DGHGTHT++T AGS V GASLLGYA GTARGMAP AR+A YKVCW+GGCF
Sbjct: 211 SKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCF 270

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDP 327
           SSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AME G+ VSCSAGN GP  
Sbjct: 271 SSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSS 330

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGSN 387
            SL+NV+PWITTVGA T+DRDFPA+  LG+G+  TGVSL++G   +P+ K  P +Y G N
Sbjct: 331 SSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGE-ALPD-KLLPFIYAG-N 390

Query: 388 SSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEE 447
           +SN    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK AGG+GMIL+NTAANGEE
Sbjct: 391 ASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEE 450

Query: 448 LVADCHLVPAVAIGEKEGKAIKQYALTNRKATATLGFLGTRLGVKPSPVVAAFSSRGPNF 507
           LVAD HL+PA  +GEK G  I+ Y  T+   TA++  LGT +GVKPSPVVAAFSSRGPN 
Sbjct: 451 LVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNS 510

Query: 508 LTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIK 567
           +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+SGTSMSCPHVSG+AAL+K
Sbjct: 511 ITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLK 570

Query: 568 SKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAGHINPRKALDPGLVY 627
           S HP+W P+AI+SALMTTAY      KPL D +   PS+P+DHGAGH++P  A +PGL+Y
Sbjct: 571 SVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIY 630

Query: 628 EIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCHRLLPNPGDLNYPAISAVFPEKTSVTSL 687
           ++  +DY  FLC  + T  Q++  S+ +         +  DLNYP+ +        V + 
Sbjct: 631 DLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV---NVDGVGAY 690

Query: 688 TLHRTVTNVGAAASSYHAVVTPFNGATVKVEPENLNFTRSYQKLSYRITFL--SKKRQSM 747
              RTVT+VG A +    V +   G  + VEP  LNF  + +K SY +TF   S K    
Sbjct: 691 KYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGS 750

Query: 748 PEFGGLIWKDGTHRVRSPIVITW 769
             FG + W DG H V SP+ I+W
Sbjct: 751 NSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Tan0016264 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 4.3e-221
Identity = 394/758 (51.98%), Postives = 511/758 (67.41%), Query Frame = 0

Query: 19  LLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGGEDRIIYSYQN 78
           LL +    KTY+++++ S  P+SF  H  WY++ L+S             E  ++Y+Y  
Sbjct: 20  LLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------ESSLLYTYTT 79

Query: 79  VFHGVAARLSEEEAER-LEEQDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIA 138
            FHG +A L   EA+  L   + +L IF +  Y LHTTR+P FLGL             +
Sbjct: 80  SFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVHDLGSS 139

Query: 139 DHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGARMFYHG 198
            + VI+GVLDTG+WPES SF+D  M  +P  WKGECE+G  F  + CN+K++GAR F  G
Sbjct: 140 SNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKG 199

Query: 199 YEAAT-GKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIA 258
           ++ A+ G F+ K E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA  AR+A
Sbjct: 200 FQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVA 259

Query: 259 AYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFV 318
            YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME GVFV
Sbjct: 260 TYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFV 319

Query: 319 SCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPEN 378
           SCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G       
Sbjct: 320 SCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGMGT 379

Query: 379 KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGM 438
           K   +VY   NSS+   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++AGG+GM
Sbjct: 380 KPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGM 439

Query: 439 ILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATATLGFLGTRLGVKPSPV 498
           I++NTAA+GEELVAD HL+PA+A+G+K G  +++Y  ++ K TA L F GT L VKPSPV
Sbjct: 440 IMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPV 499

Query: 499 VAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSC 558
           VAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SGTSMSC
Sbjct: 500 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 559

Query: 559 PHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAGHIN 618
           PH+SG+A L+K+ HP+W PSAIKSALMTTAYV DNT  PL DA+  S S+PY HG+GH++
Sbjct: 560 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 619

Query: 619 PRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCHRLLPNPGDLNYPAISA 678
           P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  + +C +   +PG LNYP+ S 
Sbjct: 620 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 679

Query: 679 VFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPENLNFTRSYQKLSYRIT 738
           +F  K  V      R VTNVGAA+S Y   V       + V+P  L+F    +K  Y +T
Sbjct: 680 LFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVT 739

Query: 739 FLSKKRQSM---PEFGGLIWKDGTHRVRSPIVITWLSF 772
           F+SKK  SM    EFG + W +  H VRSP+  +W  F
Sbjct: 740 FVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Tan0016264 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 2.2e-217
Identity = 382/754 (50.66%), Postives = 516/754 (68.44%), Query Frame = 0

Query: 28  TYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGGEDRIIYSYQNVFHGVAARL 87
           TY+V +D  A P  F  HF WY++ L+S+  +P +         II++Y  VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPS---------IIHTYDTVFHGFSARL 86

Query: 88  SEEEAERLEEQDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           + ++A +L +   V+++ PE    LHTTRSP FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGARMFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   + F +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 KLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCF 267
             E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDP 327
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP  
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGA 326

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGS- 387
           +++TNV+PW+TTVGA T+DRDFPA VKLG+G+ ++GVS+Y G    P  + +P+VY GS 
Sbjct: 327 LTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSL 386

Query: 388 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGE 447
              +   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 448 ELVADCHLVPAVAIGEKEGKAIKQYALTNRKA------TATLGFLGTRLGVKPSPVVAAF 507
            LVADCH++PA ++G   G  I++Y   + K+      TAT+ F GTRLG++P+PVVA+F
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 508 SSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVS 567
           S+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVS
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 568 GVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAGHINPRKA 627
           G+AAL+K+ HPDW P+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 628 LDPGLVYEIQPQDYFEFLCTQDLTPTQL-KVFSKYSNRSCHRLLPNPGDLNYPAISAVFP 687
           +DPGLVY+I   DY  FLC  + T T +  +  + ++    R   + G+LNYP+ S VF 
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQ 686

Query: 688 E-KTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPENLNFTRSYQKLSYRITFL 747
           +   S  S    RTVTNVG + S Y   + P  G TV VEPE L+F R  QKLS+ +   
Sbjct: 687 QYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK 746

Query: 748 SKKRQSMP-----EFGGLIWKDGTHRVRSPIVIT 768
           + + +  P     E G ++W DG   V SP+V+T
Sbjct: 747 TTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Tan0016264 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 1.3e-214
Identity = 391/772 (50.65%), Postives = 517/772 (66.97%), Query Frame = 0

Query: 9   LCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGG 68
           L L L F  +  + +Q  KT++ ++D  +MP  F  H+ WYST  +              
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE------------- 67

Query: 69  EDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRFLGLEPADS 128
           E RI++ Y  VFHG +A ++ +EA+ L     VLA+F + + ELHTTRSP+FLGL+  + 
Sbjct: 68  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQ 127

Query: 129 NSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKI 188
              WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+ +NCNRKI
Sbjct: 128 KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKI 187

Query: 189 VGARMFYHGYEAAT-GKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTAR 248
           +GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     AS+ GYA G A+
Sbjct: 188 IGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 247

Query: 249 GMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDSLS 308
           G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D ++
Sbjct: 248 GVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIA 307

Query: 309 VAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTG 368
           + ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LGDG  + G
Sbjct: 308 IGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 367

Query: 369 VSLYRGRITIPEN-KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRV 428
           VSLY G   +P N + FPVVY G   S    +SLC+E TLDP  V GKIVICDRG SPRV
Sbjct: 368 VSLYAG---VPLNGRMFPVVYPG--KSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRV 427

Query: 429 QKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATATL 488
            KG+VVK AGG+GMIL+N A+NGE LV D HL+PA A+G  EG  IK YA ++    A++
Sbjct: 428 AKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASI 487

Query: 489 GFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTR 548
            F GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D R
Sbjct: 488 DFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPR 547

Query: 549 RVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAA 608
           + +FNILSGTSM+CPHVSG AAL+KS HPDW P+ I+SA+MTT  + DN+ + L D S  
Sbjct: 548 KTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTG 607

Query: 609 SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCHRL 668
             ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R     
Sbjct: 608 KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTR 667

Query: 669 LPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPEN 728
            P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    G TV V+P  
Sbjct: 668 KPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPR 727

Query: 729 LNFTRSYQKLSYRITFLSKKR-----QSMPEFGGLIWKD-GTHRVRSPIVIT 768
           L FT + ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 728 LVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of Tan0016264 vs. NCBI nr
Match: KAG6576764.1 (Subtilisin-like protease 1.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1481.8 bits (3835), Expect = 0.0e+00
Identity = 727/772 (94.17%), Postives = 746/772 (96.63%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNP 60
           M   PLKWLCLLL F  LLLS+AQF KTYVVQMDRSAMPDSFS H QWYSTVL+SV+VNP
Sbjct: 1   MGHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ETEGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRF 120
           E EGNGGGE RIIYSYQNVFHGVAARLSE+EAERLEE+DGVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEDEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVA+GE+EGKAIK YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDAS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAYVHDNTYKPLKD+S
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCH 660
            ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTP+QLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPE 720
            LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVG A SSYHAVVTPFNGA VKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 NLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLSFV 773
           +LNFTR YQKLSY+ITFL+KKRQSMPEFGGLIWKDGTH VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Tan0016264 vs. NCBI nr
Match: XP_023552616.1 (subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1479.5 bits (3829), Expect = 0.0e+00
Identity = 727/772 (94.17%), Postives = 745/772 (96.50%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNP 60
           MA  PLKWLCLLL F  LLLS+AQF KTYVVQMDRSAMPDSFS H QWYSTVL+SV+VNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ETEGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRF 120
           E EGNGGGE RIIYSYQNVFHGVAARLSEEEAERLEE+DGVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR F+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVA+GE+EGKAIK YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDAS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAYVHDNTYKPLKD+S
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCH 660
            ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTP+QLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPE 720
            LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVG A SSYHA VTPFNGA VKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720

Query: 721 NLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLSFV 773
           +LNFTR YQKLSY+ITFL+KKRQSMPEFGGLIWKDGTH VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Tan0016264 vs. NCBI nr
Match: XP_022922929.1 (subtilisin-like protease SBT1.3 [Cucurbita moschata])

HSP 1 Score: 1478.4 bits (3826), Expect = 0.0e+00
Identity = 726/772 (94.04%), Postives = 747/772 (96.76%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNP 60
           MA  PLKWLCLLL F  LLLS+AQF KTYVVQMDRSAMPDSFS H QWYSTVL+SV+VNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ETEGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRF 120
           E EGNGGGE RIIYSYQNVFHGVAARL+EEEAERLEE++GVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPE+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVA+GE+EGKAIK YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDAS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAYVHDNTYKPLKD+S
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCH 660
            ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTP+QLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPE 720
            LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVG A SSYHAVVTPFNGA VKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 NLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLSFV 773
           +LNFTR YQKLSY+ITFL+KKRQSMPEFGGLIWKDGTH VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Tan0016264 vs. NCBI nr
Match: XP_022984431.1 (subtilisin-like protease SBT1.3 [Cucurbita maxima])

HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 723/772 (93.65%), Postives = 743/772 (96.24%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNP 60
           MA  PLKWLCLLL F   LLS+AQF KTYVVQMDRSAMPDSFS H QWYSTVL+SV+VNP
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ETEGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRF 120
           E EGNGGGE RIIYSYQNVFHGVAARLSEEEAERLEE+DGVLAIFPETKY+LHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSP A A+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVA+GE+EGKAIK YALTNRKATA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKATA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           +LGFLGT+LGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 SLGFLGTKLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDAS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAYVHDNTYKPLKD+S
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCH 660
             SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTP+QLKVFSKYSNRSCH
Sbjct: 601 GTSPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPE 720
            LLPNPGDLNYPAISAVFPEK SVTSLTLHRTVTNVG A SSYHAV TPFNGA VKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKASVTSLTLHRTVTNVGPATSSYHAVATPFNGAAVKVEPE 720

Query: 721 NLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLSFV 773
           +LNFTR YQKLSY+ITFL+KKRQSMPEFGGLIWKDGTH VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Tan0016264 vs. NCBI nr
Match: XP_023542216.1 (subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1464.9 bits (3791), Expect = 0.0e+00
Identity = 719/773 (93.01%), Postives = 749/773 (96.90%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMV-N 60
           MA  PL+WLC  LFFH LLLS+AQFFKTYVVQMDRSAMPDSFS HF+WYSTVLSSV+V N
Sbjct: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60

Query: 61  PETE--GNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRS 120
           PE E  GNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEE+ GVLA+FPE KYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PRFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGR 180
           P+FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVP +WKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGAS 240
           GFTKQNCNRKIVGAR+FYHGYEAATGKFN++LEYKSPRDQDGHGTHTAATVAGSPVAGA+
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGD 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTVTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT+TGVSLYRGRITI ENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHL+PAVA+GE EGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 ATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
            TATLG LGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNR 660
           DASAASPSSPYDHGAGHINPRKALDPGLVYEI+PQDYFEFLCTQDLTP+QLKVFSK+SNR
Sbjct: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKV 720
           SCHRLLPNPGDLNYPAISAVFPEKT++TSLTLHRTVTNVG A+SSYHA+V+PF GA VK+
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPENLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLS 771
           EPE LNFTR YQKLSYRITF++KKR SMPEFGGLIWKDG+HRVRSPI+ITWLS
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773

BLAST of Tan0016264 vs. ExPASy TrEMBL
Match: A0A6J1E4V2 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111430759 PE=3 SV=1)

HSP 1 Score: 1478.4 bits (3826), Expect = 0.0e+00
Identity = 726/772 (94.04%), Postives = 747/772 (96.76%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNP 60
           MA  PLKWLCLLL F  LLLS+AQF KTYVVQMDRSAMPDSFS H QWYSTVL+SV+VNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ETEGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRF 120
           E EGNGGGE RIIYSYQNVFHGVAARL+EEEAERLEE++GVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE+LEYKSPRDQDGHGTHTAATVAGSP AGA+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPE+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVA+GE+EGKAIK YALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDAS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAYVHDNTYKPLKD+S
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCH 660
            ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTP+QLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPE 720
            LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVG A SSYHAVVTPFNGA VKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 NLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLSFV 773
           +LNFTR YQKLSY+ITFL+KKRQSMPEFGGLIWKDGTH VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Tan0016264 vs. ExPASy TrEMBL
Match: A0A6J1J576 (subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111482733 PE=3 SV=1)

HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 723/772 (93.65%), Postives = 743/772 (96.24%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNP 60
           MA  PLKWLCLLL F   LLS+AQF KTYVVQMDRSAMPDSFS H QWYSTVL+SV+VNP
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  ETEGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRF 120
           E EGNGGGE RIIYSYQNVFHGVAARLSEEEAERLEE+DGVLAIFPETKY+LHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFT 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVP HWKGECETGR FT
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSP A A+LLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRT 360
           SLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLY+GRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATA 480
           RVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVA+GE+EGKAIK YALTNRKATA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKATA 480

Query: 481 TLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           +LGFLGT+LGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 SLGFLGTKLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDAS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAYVHDNTYKPLKD+S
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 AASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCH 660
             SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTP+QLKVFSKYSNRSCH
Sbjct: 601 GTSPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPE 720
            LLPNPGDLNYPAISAVFPEK SVTSLTLHRTVTNVG A SSYHAV TPFNGA VKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKASVTSLTLHRTVTNVGPATSSYHAVATPFNGAAVKVEPE 720

Query: 721 NLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLSFV 773
           +LNFTR YQKLSY+ITFL+KKRQSMPEFGGLIWKDGTH VRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Tan0016264 vs. ExPASy TrEMBL
Match: A0A6J1FZU3 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111449392 PE=3 SV=1)

HSP 1 Score: 1457.2 bits (3771), Expect = 0.0e+00
Identity = 715/773 (92.50%), Postives = 746/773 (96.51%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMV-N 60
           MA  PL+WLC  LFFH L  S+AQFFKTYVVQMDRSAMPDSFS H +WYSTV+SSV+V N
Sbjct: 1   MAHTPLQWLCFPLFFHCLFFSSAQFFKTYVVQMDRSAMPDSFSDHLEWYSTVVSSVVVDN 60

Query: 61  PETE--GNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRS 120
           PE E  GNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEE+ GVLA+FPE KYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PRFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGR 180
           P+FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVP +WKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGAS 240
           GFTKQNCNRKIVGAR+FYHGYEAATGKFN++LEYKSPRDQDGHGTHTAATVAGSPVAGA+
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGD 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTVTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT+TGVSLYRGRITI ENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHL+PAVA+GE EGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 ATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
            TATLG LGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNR 660
           DASAASPSSPY+HGAGHINPRKALDPGLVYEI+PQDYFEFLCTQDLTP+QLKVFSK+SNR
Sbjct: 601 DASAASPSSPYEHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKV 720
           SCHRLLPNPGDLNYPAISAVFPEKT+VTSLTLHRTVTNVG A+SSYHA+V+PF GA VK+
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPENLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLS 771
           EPE LNFTR YQKLSYRITF++KKR SMPEFGGLIWKDG+HRVRSPI+ITWLS
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773

BLAST of Tan0016264 vs. ExPASy TrEMBL
Match: A0A5A7SUB4 (Subtilisin-like protease SBT1.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00040 PE=3 SV=1)

HSP 1 Score: 1454.5 bits (3764), Expect = 0.0e+00
Identity = 713/773 (92.24%), Postives = 750/773 (97.02%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNP 60
           MA++P+K LCLLLF  SLLLS+A F KTYVVQMDRSAMP SF++HF+WYS VLS+V+V+ 
Sbjct: 1   MADSPIKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  ETEGN-GGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPR 120
           E EGN GGGE+RIIYSYQNVFHGVAARLSEEE E+LEE+DGV+AIFPE KYELHTTRSPR
Sbjct: 61  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 120

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPES+SF+DAGMSPVP HWKGECETGRGF
Sbjct: 121 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 180

Query: 181 TKQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TKQNCNRKIVGAR+FYHGY+AATGKFNE+LEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 241 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360
           DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR
Sbjct: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360

Query: 361 TVTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T++G SLYRGRITIPENKQFP+VYMGSNSS+PDPSSLCLEGTLDPH VAGKIVICDRGIS
Sbjct: 361 TMSGASLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHVVAGKIVICDRGIS 420

Query: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKAT 480
           PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVA+GEKEGKAIKQYALTNRKAT
Sbjct: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480

Query: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDA 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAY+HDNTYKPL+D+
Sbjct: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQDS 600

Query: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSC 660
           SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYF+FLCTQDLTPTQLKVFSKYSNR+C
Sbjct: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660

Query: 661 HRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEP 720
            RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVG A SSYHAVVTPF GA VKVEP
Sbjct: 661 RRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFKGAAVKVEP 720

Query: 721 ENLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLSFV 773
           E+LNFTR Y+KLSYRITF++KKR SMPEFGGLIWKDG+H+VRSPIVITWLSFV
Sbjct: 721 ESLNFTRRYEKLSYRITFVTKKRLSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773

BLAST of Tan0016264 vs. ExPASy TrEMBL
Match: A0A1S3AY00 (subtilisin-like protease SBT1.3 OS=Cucumis melo OX=3656 GN=LOC103484020 PE=3 SV=1)

HSP 1 Score: 1451.4 bits (3756), Expect = 0.0e+00
Identity = 712/773 (92.11%), Postives = 748/773 (96.77%), Query Frame = 0

Query: 1   MAEAPLKWLCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNP 60
           MA+ PLK LCLLLF  SLLLS+A F KTYVVQMDRSAMP SF++HF+WYS VLS+V+V+ 
Sbjct: 1   MADFPLKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  ETEGN-GGGEDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPR 120
           E EGN GGGE+RIIYSYQNVFHGVAARLSEEE E+LEE+DGV+AIFPE KYELHTTRSPR
Sbjct: 61  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 120

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPES+SF+DAGMSPVP HWKGECETGRGF
Sbjct: 121 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 180

Query: 181 TKQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240
           TKQNCNRKIVGAR+FYHGY+AATGKFNE+LEYKSPRDQDGHGTHTAATVAGSPVAGASLL
Sbjct: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240

Query: 241 GYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 241 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360
           DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR
Sbjct: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360

Query: 361 TVTGVSLYRGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T++G SLYRGRITIPENKQFP+VYMGSNSS+PDPSSLCLEGTLDPH VAGKIVICDRGIS
Sbjct: 361 TMSGASLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHVVAGKIVICDRGIS 420

Query: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKAT 480
           PRVQKGVVVKNAGGIGMILSNT ANGEELVADCHLVPAVA+GEKEGKAIKQYALTNRKAT
Sbjct: 421 PRVQKGVVVKNAGGIGMILSNTVANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480

Query: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDA 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDW PSAIKSALMTTAY+HDNTYKPL+D+
Sbjct: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQDS 600

Query: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSC 660
           SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYF+FLCTQDLTPTQLKVFSKYSNR+C
Sbjct: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660

Query: 661 HRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEP 720
            RLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVG A SSYHAVVTPF GA VKVEP
Sbjct: 661 RRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFKGAAVKVEP 720

Query: 721 ENLNFTRSYQKLSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWLSFV 773
           E+LNFTR Y+KLSYRITF++KKR SMPEFGGLIWKDG+H+VRSPIV+TWLSFV
Sbjct: 721 ESLNFTRRYEKLSYRITFVTKKRLSMPEFGGLIWKDGSHKVRSPIVMTWLSFV 773

BLAST of Tan0016264 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 551/759 (72.60%), Postives = 651/759 (85.77%), Query Frame = 0

Query: 12  LLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGGEDR 71
           L+F  +   +     KTYV+ MD+SAMP  +++H QWYS+ ++SV  +   E   G  +R
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE-EEGNNNR 79

Query: 72  IIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRFLGLEPADSNSA 131
           I+Y+YQ  FHG+AA+L++EEAERLEE+DGV+A+ PET+YELHTTRSP FLGLE  +S   
Sbjct: 80  ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERV 139

Query: 132 WSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGA 191
           W++++ DHDV+VGVLDTGIWPESESFND GMSPVP  W+G CETG+ F K+NCNRKIVGA
Sbjct: 140 WAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGA 199

Query: 192 RMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAP 251
           R+FY GYEAATGK +E+LEYKSPRD+DGHGTHTAATVAGSPV GA+L G+AYGTARGMA 
Sbjct: 200 RVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQ 259

Query: 252 GARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAME 311
            AR+AAYKVCWVGGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAME
Sbjct: 260 KARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAME 319

Query: 312 MGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRI 371
           MGVFVSCSAGNGGPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR 
Sbjct: 320 MGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRT 379

Query: 372 TIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNA 431
            +P+NKQ+P+VY+G N+S+PDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK A
Sbjct: 380 VLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRA 439

Query: 432 GGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATATLGFLGTRLGV 491
           GGIGM+L+NTA NGEELVAD H++PAVA+GEKEGK IKQYA+T++KATA+L  LGTR+G+
Sbjct: 440 GGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGI 499

Query: 492 KPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSG 551
           KPSPVVAAFSSRGPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSG
Sbjct: 500 KPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSG 559

Query: 552 TSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHG 611
           TSMSCPHVSGVAALIKS+HPDW P+AIKSALMTTAYVHDN +KPL DAS A+PSSPYDHG
Sbjct: 560 TSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHG 619

Query: 612 AGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSC-HRLLPNPGDLN 671
           AGHI+P +A DPGLVY+I PQ+YFEFLCTQDL+P+QLKVF+K+SNR+C H L  NPG+LN
Sbjct: 620 AGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLN 679

Query: 672 YPAISAVFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPENLNFTRSYQK 731
           YPAISA+FPE T V ++TL RTVTNVG   SSY   V+PF GA+V V+P+ LNFT  +QK
Sbjct: 680 YPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQK 739

Query: 732 LSYRITFLSKKRQSMPEFGGLIWKDGTHRVRSPIVITWL 770
           LSY +TF ++ R   PEFGGL+WK  TH+VRSP++ITWL
Sbjct: 740 LSYTVTFRTRFRMKRPEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Tan0016264 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 811.2 bits (2094), Expect = 7.0e-235
Identity = 405/743 (54.51%), Postives = 520/743 (69.99%), Query Frame = 0

Query: 28  TYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGGEDRIIYSYQNVFHGVAARL 87
           TY+V M +S MP SF  H  WY + L S+  + E          ++Y+Y+N  HG + RL
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE----------LLYTYENAIHGFSTRL 90

Query: 88  SEEEAERLEEQDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           ++EEA+ L  Q GV+++ PE +YELHTTR+P FLGL+   ++  + +  +  DV+VGVLD
Sbjct: 91  TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD-LFPEAGSYSDVVVGVLD 150

Query: 148 TGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGARMFYHGYEAATGKFNE 207
           TG+WPES+S++D G  P+P  WKG CE G  FT   CNRK++GAR F  GYE+  G  +E
Sbjct: 151 TGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDE 210

Query: 208 KLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCF 267
             E +SPRD DGHGTHT++T AGS V GASLLGYA GTARGMAP AR+A YKVCW+GGCF
Sbjct: 211 SKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCF 270

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDP 327
           SSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AME G+ VSCSAGN GP  
Sbjct: 271 SSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSS 330

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGSN 387
            SL+NV+PWITTVGA T+DRDFPA+  LG+G+  TGVSL++G   +P+ K  P +Y G N
Sbjct: 331 SSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGE-ALPD-KLLPFIYAG-N 390

Query: 388 SSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEE 447
           +SN    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK AGG+GMIL+NTAANGEE
Sbjct: 391 ASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEE 450

Query: 448 LVADCHLVPAVAIGEKEGKAIKQYALTNRKATATLGFLGTRLGVKPSPVVAAFSSRGPNF 507
           LVAD HL+PA  +GEK G  I+ Y  T+   TA++  LGT +GVKPSPVVAAFSSRGPN 
Sbjct: 451 LVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNS 510

Query: 508 LTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIK 567
           +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+SGTSMSCPHVSG+AAL+K
Sbjct: 511 ITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLK 570

Query: 568 SKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAGHINPRKALDPGLVY 627
           S HP+W P+AI+SALMTTAY      KPL D +   PS+P+DHGAGH++P  A +PGL+Y
Sbjct: 571 SVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIY 630

Query: 628 EIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCHRLLPNPGDLNYPAISAVFPEKTSVTSL 687
           ++  +DY  FLC  + T  Q++  S+ +         +  DLNYP+ +        V + 
Sbjct: 631 DLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAV---NVDGVGAY 690

Query: 688 TLHRTVTNVGAAASSYHAVVTPFNGATVKVEPENLNFTRSYQKLSYRITFL--SKKRQSM 747
              RTVT+VG A +    V +   G  + VEP  LNF  + +K SY +TF   S K    
Sbjct: 691 KYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGS 750

Query: 748 PEFGGLIWKDGTHRVRSPIVITW 769
             FG + W DG H V SP+ I+W
Sbjct: 751 NSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Tan0016264 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 769.2 bits (1985), Expect = 3.1e-222
Identity = 394/758 (51.98%), Postives = 511/758 (67.41%), Query Frame = 0

Query: 19  LLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGGEDRIIYSYQN 78
           LL +    KTY+++++ S  P+SF  H  WY++ L+S             E  ++Y+Y  
Sbjct: 20  LLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------ESSLLYTYTT 79

Query: 79  VFHGVAARLSEEEAER-LEEQDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIA 138
            FHG +A L   EA+  L   + +L IF +  Y LHTTR+P FLGL             +
Sbjct: 80  SFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEFGVHDLGSS 139

Query: 139 DHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGARMFYHG 198
            + VI+GVLDTG+WPES SF+D  M  +P  WKGECE+G  F  + CN+K++GAR F  G
Sbjct: 140 SNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKG 199

Query: 199 YEAAT-GKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIA 258
           ++ A+ G F+ K E  SPRD DGHGTHT+ T AGS V  AS LGYA GTARGMA  AR+A
Sbjct: 200 FQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVA 259

Query: 259 AYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFV 318
            YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF AME GVFV
Sbjct: 260 TYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFV 319

Query: 319 SCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPEN 378
           SCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LG+G+ +TGVSLY G       
Sbjct: 320 SCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGMGT 379

Query: 379 KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGM 438
           K   +VY   NSS+   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV++AGG+GM
Sbjct: 380 KPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGM 439

Query: 439 ILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATATLGFLGTRLGVKPSPV 498
           I++NTAA+GEELVAD HL+PA+A+G+K G  +++Y  ++ K TA L F GT L VKPSPV
Sbjct: 440 IMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPV 499

Query: 499 VAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSC 558
           VAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI+SGTSMSC
Sbjct: 500 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 559

Query: 559 PHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAGHIN 618
           PH+SG+A L+K+ HP+W PSAIKSALMTTAYV DNT  PL DA+  S S+PY HG+GH++
Sbjct: 560 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 619

Query: 619 PRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCHRLLPNPGDLNYPAISA 678
           P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  + +C +   +PG LNYP+ S 
Sbjct: 620 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 679

Query: 679 VFPEKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPENLNFTRSYQKLSYRIT 738
           +F  K  V      R VTNVGAA+S Y   V       + V+P  L+F    +K  Y +T
Sbjct: 680 LFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVT 739

Query: 739 FLSKKRQSM---PEFGGLIWKDGTHRVRSPIVITWLSF 772
           F+SKK  SM    EFG + W +  H VRSP+  +W  F
Sbjct: 740 FVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Tan0016264 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 756.9 bits (1953), Expect = 1.6e-218
Identity = 382/754 (50.66%), Postives = 516/754 (68.44%), Query Frame = 0

Query: 28  TYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGGEDRIIYSYQNVFHGVAARL 87
           TY+V +D  A P  F  HF WY++ L+S+  +P +         II++Y  VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPS---------IIHTYDTVFHGFSARL 86

Query: 88  SEEEAERLEEQDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           + ++A +L +   V+++ PE    LHTTRSP FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKIVGARMFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   + F +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 KLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTARGMAPGARIAAYKVCWVGGCF 267
             E++SPRD DGHGTHTA+  AG  V  AS LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDP 327
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGNGGP  
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGA 326

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTGVSLYRGRITIPENKQFPVVYMGS- 387
           +++TNV+PW+TTVGA T+DRDFPA VKLG+G+ ++GVS+Y G    P  + +P+VY GS 
Sbjct: 327 LTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSL 386

Query: 388 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAANGE 447
              +   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI++N   +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 448 ELVADCHLVPAVAIGEKEGKAIKQYALTNRKA------TATLGFLGTRLGVKPSPVVAAF 507
            LVADCH++PA ++G   G  I++Y   + K+      TAT+ F GTRLG++P+PVVA+F
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 508 SSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVS 567
           S+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVS
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 568 GVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAGHINPRKA 627
           G+AAL+K+ HPDW P+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 628 LDPGLVYEIQPQDYFEFLCTQDLTPTQL-KVFSKYSNRSCHRLLPNPGDLNYPAISAVFP 687
           +DPGLVY+I   DY  FLC  + T T +  +  + ++    R   + G+LNYP+ S VF 
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQ 686

Query: 688 E-KTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPENLNFTRSYQKLSYRITFL 747
           +   S  S    RTVTNVG + S Y   + P  G TV VEPE L+F R  QKLS+ +   
Sbjct: 687 QYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK 746

Query: 748 SKKRQSMP-----EFGGLIWKDGTHRVRSPIVIT 768
           + + +  P     E G ++W DG   V SP+V+T
Sbjct: 747 TTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Tan0016264 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 747.7 bits (1929), Expect = 9.6e-216
Identity = 391/772 (50.65%), Postives = 517/772 (66.97%), Query Frame = 0

Query: 9   LCLLLFFHSLLLSNAQFFKTYVVQMDRSAMPDSFSHHFQWYSTVLSSVMVNPETEGNGGG 68
           L L L F  +  + +Q  KT++ ++D  +MP  F  H+ WYST  +              
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE------------- 67

Query: 69  EDRIIYSYQNVFHGVAARLSEEEAERLEEQDGVLAIFPETKYELHTTRSPRFLGLEPADS 128
           E RI++ Y  VFHG +A ++ +EA+ L     VLA+F + + ELHTTRSP+FLGL+  + 
Sbjct: 68  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQ 127

Query: 129 NSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPPHWKGECETGRGFTKQNCNRKI 188
              WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  F+ +NCNRKI
Sbjct: 128 KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKI 187

Query: 189 VGARMFYHGYEAAT-GKFNEKLEYKSPRDQDGHGTHTAATVAGSPVAGASLLGYAYGTAR 248
           +GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     AS+ GYA G A+
Sbjct: 188 IGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 247

Query: 249 GMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDSLS 308
           G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D ++
Sbjct: 248 GVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIA 307

Query: 309 VAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGRTVTG 368
           + ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LGDG  + G
Sbjct: 308 IGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 367

Query: 369 VSLYRGRITIPEN-KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRV 428
           VSLY G   +P N + FPVVY G   S    +SLC+E TLDP  V GKIVICDRG SPRV
Sbjct: 368 VSLYAG---VPLNGRMFPVVYPG--KSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRV 427

Query: 429 QKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAIGEKEGKAIKQYALTNRKATATL 488
            KG+VVK AGG+GMIL+N A+NGE LV D HL+PA A+G  EG  IK YA ++    A++
Sbjct: 428 AKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASI 487

Query: 489 GFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTR 548
            F GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D R
Sbjct: 488 DFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPR 547

Query: 549 RVKFNILSGTSMSCPHVSGVAALIKSKHPDWGPSAIKSALMTTAYVHDNTYKPLKDASAA 608
           + +FNILSGTSM+CPHVSG AAL+KS HPDW P+ I+SA+MTT  + DN+ + L D S  
Sbjct: 548 KTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTG 607

Query: 609 SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPTQLKVFSKYSNRSCHRL 668
             ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R     
Sbjct: 608 KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTR 667

Query: 669 LPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGAAASSYHAVVTPFNGATVKVEPEN 728
            P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    G TV V+P  
Sbjct: 668 KPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPR 727

Query: 729 LNFTRSYQKLSYRITFLSKKR-----QSMPEFGGLIWKD-GTHRVRSPIVIT 768
           L FT + ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 728 LVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759