Tan0015845 (gene) Snake gourd v1

Overview
NameTan0015845
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionbeta-fructofuranosidase, insoluble isoenzyme CWINV1-like
LocationLG06: 1476144 .. 1480798 (-)
RNA-Seq ExpressionTan0015845
SyntenyTan0015845
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTTCAATGGCTTCCTTTTCTCTCTGGTTCATCTCTTTCCTCTGTCTCTTATTTGGCCATGGAATTTTGAAGCTTCAAGCTTCCCACCATGTTTATAAAGCTCAAGCTTCTCAATTTTTTCCTCATCATTATCAACAGCCCTATAGAACTTCTTACCATTTTCAGCCTCCCAAGAATTGGATGAATGGTTTGTAACTTTTTTTTTTTCCTTCAATATATTTTTCATTTTTTTTGGCTTATGGGTTTTTTTTTTTTTAAATTTCCTTCTTCTAAAAGTTTTCAACTTTATTTTTCTTTGACATGTGATTTTGGCAATGGAAAAACTCAGATCCAAATGGTGAGTTCATCTCTTCTCTGTTCTTCATTTTTTTTTTTAATTTTTGCTGCATTTGTCAAATTAATTTCATTACTACATAAAAATTAGAAATTATAAATTTATAATTAACCTCCATTTTTATTTCATTGCTATATTTCCATTTTTGGGAAATTTCAGGTCCAATGATATACAAGGGAATTTATCATCTATTTTACCAATACAATCCAAAAGGAGCAGTTTGGGGCAACATAGTGTGGGCCCACTCAACATCAACGGACCTGATCAACTGGGTGCCCCACGAGCATGCCATCTACCCATCTCAGCCGTCCGATATTAACGGCTGCTGGTCGGGCTCCGCCACCATTCTTCCCGGCGGCAAACCGGCGATTTTGTACACAGGAATTAACCCTAAAAACCAACAAGTTCAAAACTTGGCTGTCCCTAAAAATCTCTCTGATCCATATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTCATGGCCCCCACCCCCCTCAACCACATCAACTCTAGCTCTTTCCGGGACCCCACCACTGCCTGGCTCGGCCCCGATCGGAAATGGCGAGTCATCATCGGGAGCAAGATTAACACCCGTGGACTCGCCATCATGTATCGGAGCAAGGATTTCATTCAGTGGGATCAGGTAACTTGTTTAAACTACCACTAAACTCAAAAGTTTATTCAGCCTTTCCTAGAGCATTTGATAGAGTCAGAATCCCTAGCTATAGGGTTTGTGTCATTCGAAGTTTGAAAAGATTTTCACGAAACAAACCATGGATTTTGAACACAGTAACTCAAACACCGTTTTGAATTTGAATTTCAAATCCAGTCAAACTCATCACTATTCAAAACCACCTGGTTGCATGAATTTTGAATACAGTGACCCTAATACCATTTTGCATTTGGATTTCAAAACCAGCCAAACTCATCCAAATCTAAATTGGTGGCTTTAACGCCCTCTTTTGTGGTGGGTTGTTGAGGTAAGAAGATTCGAACCTCTAATACATATATCAATTAATTATCGTTCAACTATACTCACTTAGGTAATTTTATATGTTGATAATGATTTATTTATTTATTTATGTTGTGAACAGGTTGATCATCCACTTCATTATGGCGATGGCATGGGAATGTGGGAATGCCCAGATTTCTTCCCAGTAGCCAAAAATGGTAGTCGAAGTGGCATTGATACCACAGTCACAGTGAGTGGATGGCACGTGAAGCACGTGCTGAAGATGAGCTTGGACGATACAAAGCACGATATTTATATTATTGGAAGATATGATTTGGAAAAAGAAATTTATGTTCCAGATAAAGGATCCATAGAAAATTACAATGGATTGAGATATGATTATGGAAAATATTATGCTTCAAAAACTTTCTTTGATACCCAAAACAAAAGGAGAGTTTTATGGGGTTGGGTCAATGAATCTTCAAGTGTTCATGATGATATCAAGAAAGGATGGTCTGGACTTCAGGTAATTATTTATTTATTTAATTAATTAAAGTCAATCATTCAATTCAACCTTCACTTTTCATCTTTGAAAAAAAAAAAACTTATGCTATAAAAAAAATACAAAAGAAAAGTCTTCATCTGCCCACATCCACATCAAGAAGTGATAAGATTTGAATTCTGTTTTCTATGATTTCGTTTATAATTTTTTGATTTTTTAAAATTATACATTTCCCTTCATATTCTCTATAAAGATTTCTATCTTCTTTTAGGTACGTACCATTTACGTTTTTAGCTAAATTTCAAAAACAAAAACAAGTTTTTCAAAAGTACTTTTTTTTTTTTTAATTTTTAAAACTTAATTTGATTTTTGAAAATATAAGTTCAAAGTAGATAAGAAAACATAGAAACTCATTAGTAGAAGTTGTATTTTTAGGTTTAATTTTTAAAAGTCAAAGTTCTGCTCTATAAATTATATCACTATTTAACATTTTTTTAAAAACAGTTATTTTGGTTCTAACTACTATCTTATTATATAATATCCTTACTGTCGTGCCTCTACCAATATATATGTGACACTTATACATATTCATGTTAGTCTTTGCCATACATACTTGAAGTTACCTTATCTATTTTGTTAAAAGAAAGATAAAAGGATAAATATCAAAATAATTATTTCTTCTCTTCAAGTATGGAGATTAAATATTTCGATAATTCAAAACTAAAATGGAATAGACTAAAATTGATATTTTAAAAAGAGCACAACTTGAGTATAGCCTGAAATGCAATCAAGTATTGTGTGGTCTACTTTATCACCATAATTTTTTTTTTAAAAAAAGAATTATATTTTTTTTTGTAGTACTCTAGTGGGTTAACCCACTAATACTTAAGTACAACTTTATTTTTTCCTTTTAGAAATTCAAGATCTAAAATGGAATTTAAATATAAAGAATAATGATATTGTATTATAGGTTGGGGTAGGTTGGGGCACAGTTTTTCATAGTAGACTCACATGTGGGGTTGAATGAGAGACCATTGCATTCACATTTTGCAGGCAATTCCAAGGACTATTTGGTTGGACACATCTAGAAAGCAACTAATTCAATGGCCAATTGTTGAAATTCAAAAACTTCGAAAGGACAAACTTAAGTTGACAAACAAAGTTGTGAAGAAAGGATCAACGATCGAAATTCAGGGTATAACAGCAGCACAGGTAACTAAATTATTAGTCATGTCTTACAATTCGTTTGAAATAACTTTTCAAGTGATTAAAAAAAAGTTTTTAAGCACTTGAAAAGTCATTCCAAACAAACTCTTACCCTTGGTGAAACATTATAAAGTCTGTTTGATTTTGTTTTTGGGTTTCTTTTTACAGGCCGATATTGAGGTTTCATTTAAAGTAAGAAACTTTAAGAAAGCTGAAGTTTTGAAACCAGAATGGAAAGACCCACAATTGCTTTGTAGCAAAAAGGGAACAGAGGAAGGTGGAGTTGGCCCATTTGGGCTTCTAGTTTTAGCTTCAAAGGACTTAAAAGAGTACACTTCAGTGTTCTTTAGGATATTCAAAGGCCACAACAAATATGTCGTGCTTATGTGTAGTGACCAAAGCAGGTACGAAATAATTATTTACGACATTACAACAATAACGTGACTTATAATAGTTTTATACGGTTTGATTTGTTTCGATAGAAATTTTTTTAAAAAATAATAATAGTGAATCAAATAGTTAAGAAAGTACAACAACATTGTGATAGCCAAATTGAACCATTTATTTCTGATTTTTTTTTTTTTGTCATGACTAGGTCTTCATTGAACTCAAATAATGACAAGACAACATATGGGGCATTCTTGGATGTGGACCCACTTCGTGAAAATGTTTCACTAAGAAGCTTGGTAAGATTCTTTTTCCATTCATTTGCAAATTTATGTGGGTTTTTTCCTTTTTTTTTTAAATTTATATCCACCATGATTTTTTATAATATAATGATGATTTTTTATAATTTAATGAATGCCTCGAGTTCTGGGGCTTTGCTTTTAGGACAATTTTAAAAAGTGCACTTACCCATGTGTTCAAACACGATTTGTTAATCAATTAAATTAAATTTTTTTTTAAAAAAAAGATCCATAAGGTTGTGTTGTGTGACACTTTTGGTTGGTGAAGCCTTTTGAGGATTATTGAGGTGGGCCATGAGCATAACACATAAATGATAAAACGAGTCTCGAGATCCATAATGATTATTAAATTAAAAAAAATCAATCTCGAACGATTTTTTAGAAATAAAGTTTTAGGTTTATTCAAATGAATGATTTAAAGAGTTATTGATTCTAACACTTGATTGTATAGTTGATCATGTGAACCTTTTAATTCATATGCATAGATCTCGAATCTATAAAAGTGGTTGTTCTCGAAATTCGAGTGGAGAAAAAACGAGTCATTAGACAAAAAGAAAAGAAGTTCAGGTCGAATTTATTAATATTATATTTATGTAAGCATTTGAGCATTTTTGTCAACTAATGCACATGAAAAACTATGAAACTTCAACAGATTGATCACTCAATTGTTGAGAGCTTTGGATCTAAAGGGAAGGCTTGCATTACAGCCAGAGTTTATCCAACATTGGCCATTGGTGAAAATGTTGGTCTTTATGTTTTCAACAATGGAACAGAGGCTGTCGCCATCACAAAACTGAATGCTTGGAGCATGAACAAAGCTCAAATCAATTGAACCTAAAAGAGCCAATAACTGGGAAGGAAATGAGGGAGAGAGGGAGACTTTTTACCATTTTTTTCTTCTCATTTTTACTGTGCATTTTGATAAGCCAATTGGCAGTTAAATAAAAGATCTAATTTACTTTTATTTAA

mRNA sequence

CTTCTTCAATGGCTTCCTTTTCTCTCTGGTTCATCTCTTTCCTCTGTCTCTTATTTGGCCATGGAATTTTGAAGCTTCAAGCTTCCCACCATGTTTATAAAGCTCAAGCTTCTCAATTTTTTCCTCATCATTATCAACAGCCCTATAGAACTTCTTACCATTTTCAGCCTCCCAAGAATTGGATGAATGATCCAAATGGTCCAATGATATACAAGGGAATTTATCATCTATTTTACCAATACAATCCAAAAGGAGCAGTTTGGGGCAACATAGTGTGGGCCCACTCAACATCAACGGACCTGATCAACTGGGTGCCCCACGAGCATGCCATCTACCCATCTCAGCCGTCCGATATTAACGGCTGCTGGTCGGGCTCCGCCACCATTCTTCCCGGCGGCAAACCGGCGATTTTGTACACAGGAATTAACCCTAAAAACCAACAAGTTCAAAACTTGGCTGTCCCTAAAAATCTCTCTGATCCATATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTCATGGCCCCCACCCCCCTCAACCACATCAACTCTAGCTCTTTCCGGGACCCCACCACTGCCTGGCTCGGCCCCGATCGGAAATGGCGAGTCATCATCGGGAGCAAGATTAACACCCGTGGACTCGCCATCATGTATCGGAGCAAGGATTTCATTCAGTGGGATCAGGTTGATCATCCACTTCATTATGGCGATGGCATGGGAATGTGGGAATGCCCAGATTTCTTCCCAGTAGCCAAAAATGGTAGTCGAAGTGGCATTGATACCACAGTCACAGTGAGTGGATGGCACGTGAAGCACGTGCTGAAGATGAGCTTGGACGATACAAAGCACGATATTTATATTATTGGAAGATATGATTTGGAAAAAGAAATTTATGTTCCAGATAAAGGATCCATAGAAAATTACAATGGATTGAGATATGATTATGGAAAATATTATGCTTCAAAAACTTTCTTTGATACCCAAAACAAAAGGAGAGTTTTATGGGGTTGGGTCAATGAATCTTCAAGTGTTCATGATGATATCAAGAAAGGATGGTCTGGACTTCAGGCAATTCCAAGGACTATTTGGTTGGACACATCTAGAAAGCAACTAATTCAATGGCCAATTGTTGAAATTCAAAAACTTCGAAAGGACAAACTTAAGTTGACAAACAAAGTTGTGAAGAAAGGATCAACGATCGAAATTCAGGGTATAACAGCAGCACAGGCCGATATTGAGGTTTCATTTAAAGTAAGAAACTTTAAGAAAGCTGAAGTTTTGAAACCAGAATGGAAAGACCCACAATTGCTTTGTAGCAAAAAGGGAACAGAGGAAGGTGGAGTTGGCCCATTTGGGCTTCTAGTTTTAGCTTCAAAGGACTTAAAAGAGTACACTTCAGTGTTCTTTAGGATATTCAAAGGCCACAACAAATATGTCGTGCTTATGTGTAGTGACCAAAGCAGGTCTTCATTGAACTCAAATAATGACAAGACAACATATGGGGCATTCTTGGATGTGGACCCACTTCGTGAAAATGTTTCACTAAGAAGCTTGATTGATCACTCAATTGTTGAGAGCTTTGGATCTAAAGGGAAGGCTTGCATTACAGCCAGAGTTTATCCAACATTGGCCATTGGTGAAAATGTTGGTCTTTATGTTTTCAACAATGGAACAGAGGCTGTCGCCATCACAAAACTGAATGCTTGGAGCATGAACAAAGCTCAAATCAATTGAACCTAAAAGAGCCAATAACTGGGAAGGAAATGAGGGAGAGAGGGAGACTTTTTACCATTTTTTTCTTCTCATTTTTACTGTGCATTTTGATAAGCCAATTGGCAGTTAAATAAAAGATCTAATTTACTTTTATTTAA

Coding sequence (CDS)

ATGGCTTCCTTTTCTCTCTGGTTCATCTCTTTCCTCTGTCTCTTATTTGGCCATGGAATTTTGAAGCTTCAAGCTTCCCACCATGTTTATAAAGCTCAAGCTTCTCAATTTTTTCCTCATCATTATCAACAGCCCTATAGAACTTCTTACCATTTTCAGCCTCCCAAGAATTGGATGAATGATCCAAATGGTCCAATGATATACAAGGGAATTTATCATCTATTTTACCAATACAATCCAAAAGGAGCAGTTTGGGGCAACATAGTGTGGGCCCACTCAACATCAACGGACCTGATCAACTGGGTGCCCCACGAGCATGCCATCTACCCATCTCAGCCGTCCGATATTAACGGCTGCTGGTCGGGCTCCGCCACCATTCTTCCCGGCGGCAAACCGGCGATTTTGTACACAGGAATTAACCCTAAAAACCAACAAGTTCAAAACTTGGCTGTCCCTAAAAATCTCTCTGATCCATATCTAAGAGAATGGGTGAAATCCCCAAAAAACCCTCTCATGGCCCCCACCCCCCTCAACCACATCAACTCTAGCTCTTTCCGGGACCCCACCACTGCCTGGCTCGGCCCCGATCGGAAATGGCGAGTCATCATCGGGAGCAAGATTAACACCCGTGGACTCGCCATCATGTATCGGAGCAAGGATTTCATTCAGTGGGATCAGGTTGATCATCCACTTCATTATGGCGATGGCATGGGAATGTGGGAATGCCCAGATTTCTTCCCAGTAGCCAAAAATGGTAGTCGAAGTGGCATTGATACCACAGTCACAGTGAGTGGATGGCACGTGAAGCACGTGCTGAAGATGAGCTTGGACGATACAAAGCACGATATTTATATTATTGGAAGATATGATTTGGAAAAAGAAATTTATGTTCCAGATAAAGGATCCATAGAAAATTACAATGGATTGAGATATGATTATGGAAAATATTATGCTTCAAAAACTTTCTTTGATACCCAAAACAAAAGGAGAGTTTTATGGGGTTGGGTCAATGAATCTTCAAGTGTTCATGATGATATCAAGAAAGGATGGTCTGGACTTCAGGCAATTCCAAGGACTATTTGGTTGGACACATCTAGAAAGCAACTAATTCAATGGCCAATTGTTGAAATTCAAAAACTTCGAAAGGACAAACTTAAGTTGACAAACAAAGTTGTGAAGAAAGGATCAACGATCGAAATTCAGGGTATAACAGCAGCACAGGCCGATATTGAGGTTTCATTTAAAGTAAGAAACTTTAAGAAAGCTGAAGTTTTGAAACCAGAATGGAAAGACCCACAATTGCTTTGTAGCAAAAAGGGAACAGAGGAAGGTGGAGTTGGCCCATTTGGGCTTCTAGTTTTAGCTTCAAAGGACTTAAAAGAGTACACTTCAGTGTTCTTTAGGATATTCAAAGGCCACAACAAATATGTCGTGCTTATGTGTAGTGACCAAAGCAGGTCTTCATTGAACTCAAATAATGACAAGACAACATATGGGGCATTCTTGGATGTGGACCCACTTCGTGAAAATGTTTCACTAAGAAGCTTGATTGATCACTCAATTGTTGAGAGCTTTGGATCTAAAGGGAAGGCTTGCATTACAGCCAGAGTTTATCCAACATTGGCCATTGGTGAAAATGTTGGTCTTTATGTTTTCAACAATGGAACAGAGGCTGTCGCCATCACAAAACTGAATGCTTGGAGCATGAACAAAGCTCAAATCAATTGA

Protein sequence

MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMNDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCWSGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHINSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMWECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDKGSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTIWLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFKKAEVLKPEWKDPQLLCSKKGTEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYPTLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN
Homology
BLAST of Tan0015845 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 6.3e-233
Identity = 387/581 (66.61%), Postives = 462/581 (79.52%), Query Frame = 0

Query: 2   ASFSLWFISFLCLLFGHGILKLQASHHVYK--AQASQFFPHHYQQPYRTSYHFQPPKNWM 61
           ++  LW +  L LL G+ ++ L+ASHHVYK   Q++        QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGC 121
           NDPNGPMIYKGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNH 181
           WSGSATILP GKP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP  +N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGM 241
           IN+SSFRDPTTAWLG D+KWRVIIGSKI+ RGLAI Y SKDF++W++   PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPD 301
           WECPDFFPV + GS     ++       +KHVLK+SLDDTKHD Y IG YD  K+ +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRT 361
            G   +    RYDYGKYYASKTFFD+   RR+LWGW NESSSV DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 IWLDTSRKQLIQWPIVEIQKLRKDKLK-LTNKVVKKGSTIEIQGITAAQADIEVSFKVRN 421
           IWLD S KQLIQWP+ E+++LR  ++K L NKV+K GS +E+ G+TAAQAD+EV FKVR+
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FKKAEVLKPEWKDPQLLCSKKGTE-EGGVGPFGLLVLASKDLKEYTSVFFRIFKGH---N 481
            +KA+V++P W DPQL+CSK     + G+GPFGL+VLASK+L+EYTSV+FRIFK     N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACIT 541
           KYVVLMCSDQSRSSL  +NDKTTYGAF+D++P  + +SLR+LIDHS+VESFG KG+ACIT
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACIT 546

Query: 542 ARVYPTLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           +RVYP LAIG++  L+ FN G ++V +  LNAWSMN AQI+
Sbjct: 547 SRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of Tan0015845 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 2.7e-212
Identity = 354/555 (63.78%), Postives = 432/555 (77.84%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHH----YQQPYRTSYHFQPPK 60
           MA  S++ +S   L++   ++ ++A+HHVY+        HH      QPYRT+YHFQP K
Sbjct: 1   MAISSIFLLSLFSLIY---VIPIEATHHVYQT-LETLSSHHSSKSNHQPYRTAYHFQPLK 60

Query: 61  NWMNDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDI 120
           NW+NDPNGPM Y G YHLFYQYNPKGAVWGNIVWAHS S DL+NW P +HAI+PSQPSDI
Sbjct: 61  NWINDPNGPMRYGGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDHAIHPSQPSDI 120

Query: 121 NGCWSGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP 180
            GCWSGSATILPGGKPAILYTGI+P N QVQN+A+PKN+SDP LREW KSPKNPLM PT 
Sbjct: 121 KGCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWKKSPKNPLMEPTI 180

Query: 181 LNHINSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDG 240
            N INSSSFRDPTT+WLG D  WRV+IGSKI+T+G+AI+Y+SK+F+ W +  HPLH  +G
Sbjct: 181 ANKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDWVEAKHPLHSAEG 240

Query: 241 MGMWECPDFFPVA-KNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEI 300
            GMWECPDF+PV  KN  R+G+DT+       V+HVLK+SLDDTKHD Y+IG YD+ K++
Sbjct: 241 TGMWECPDFYPVLDKNLLRTGVDTSRNGDD-DVRHVLKVSLDDTKHDHYLIGSYDVVKDV 300

Query: 301 YVPDKGSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQA 360
           +VP+ G  +N   LRYDYGKYYASKTFFD    RR+L GWVNESSSV DD+KKGWSG+  
Sbjct: 301 FVPENGFEDNGFVLRYDYGKYYASKTFFDDGKNRRILLGWVNESSSVADDVKKGWSGIHT 360

Query: 361 IPRTIWLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFK 420
           IPR IWL  S KQL+QWP+ EI+ LR + +    KV+K G  I I G+ + QAD+E+SF+
Sbjct: 361 IPREIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGGERISITGVDSVQADVEISFE 420

Query: 421 VRNFKKAEVLKPEWKDPQLLCSKKGT-EEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHN 480
           + +  K E L+ +W DPQLLCS+KG   +GGVGPFGLLV AS+ LKEYT+VFFRIFK  +
Sbjct: 421 ISDLGKVESLR-KWIDPQLLCSQKGAGVKGGVGPFGLLVFASQGLKEYTAVFFRIFKYQD 480

Query: 481 KYVVLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACIT 540
           K +VLMCSDQSRSSLN +ND T+YG F+DVDPL E +SLR+LIDHS+VESFG +G+AC+T
Sbjct: 481 KNLVLMCSDQSRSSLNKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRACVT 540

Query: 541 ARVYPTLAIGENVGL 550
           ARVYPTLAI +   L
Sbjct: 541 ARVYPTLAIHDKAML 549

BLAST of Tan0015845 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 4.4e-210
Identity = 362/578 (62.63%), Postives = 435/578 (75.26%), Query Frame = 0

Query: 9   ISFLCLLFGHG---ILKLQASHHVYKAQASQFFPHHY----QQPYRTSYHFQPPKNWMND 68
           +S   +L  HG   I K QA HH            H       PYRT+YHFQ PKNWMND
Sbjct: 11  LSISMMLLSHGMIIIAKDQAIHHHDDDHDDMLINDHQMINDDDPYRTAYHFQSPKNWMND 70

Query: 69  PNGPMIYKGIYHLFYQYNPKGAVW-GNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 128
           PNGPMIYKGIYHLFYQY P   VW   IVW HSTSTDLINW     A+ PS+P DINGCW
Sbjct: 71  PNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSPSEPYDINGCW 130

Query: 129 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPL--N 188
           SGS TILP  KP ILYTGIN KN QVQNLA+PKNLSDPYL+EW+K P+NPLMA TP   N
Sbjct: 131 SGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNPLMAGTPTNNN 190

Query: 189 HINSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMG 248
           +IN+SSFRDP+TAW   D KWRVI+G++   RGLA+++ S DF++W+   +PLH  +G G
Sbjct: 191 NINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVKWNNTGNPLHSTEGNG 250

Query: 249 MWECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVP 308
           +WECPDFFPV   G   G DT++   G  VKHVLK+SL DT+++ Y IGRYD+EK+IYVP
Sbjct: 251 IWECPDFFPVYV-GKSLGADTSII--GDDVKHVLKLSLFDTQYEYYTIGRYDIEKDIYVP 310

Query: 309 DKGSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPR 368
           D+GSIE+  GLRYDYGK+YASK+FFD +  RR+LWGWVNESS   DDIKKGWSG+QAIPR
Sbjct: 311 DEGSIESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSIQADDIKKGWSGVQAIPR 370

Query: 369 TIWLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRN 428
           T+ LD S KQL+QWP+ E+  LR++ ++L ++V+K GS +EI  ITA+QAD+E+SFK+  
Sbjct: 371 TVVLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEISQITASQADVEISFKIPE 430

Query: 429 FKKAEVLKPEWKDPQLLCSKKGTE-EGGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYV 488
               E L     +PQ+LCS+KG   +G  GPFGLL LAS  L+EYT+VFFRIFKG NKYV
Sbjct: 431 SNYVEELDSTCTNPQILCSQKGASIKGRFGPFGLLTLASMGLEEYTAVFFRIFKGPNKYV 490

Query: 489 VLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARV 548
           VLMCSDQ+RSSLN   DK ++G F+DVDP+ E++SLR LIDHSIVESF +KGK+CITARV
Sbjct: 491 VLMCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILIDHSIVESFSAKGKSCITARV 550

Query: 549 YPTLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           YPT+AI +   LYVFNNGTE V ITKL+AWSM KAQIN
Sbjct: 551 YPTMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQIN 585

BLAST of Tan0015845 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 725.3 bits (1871), Expect = 5.3e-208
Identity = 351/573 (61.26%), Postives = 425/573 (74.17%), Query Frame = 0

Query: 12  LCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMNDPNGPMIYKGI 71
           L +   + I  L+AS H               QPYRT YHFQP KNWMNDPNGPMIYKGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCWSGSATILPGG 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWSGS TILP G
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHINSSSFRDPTT 191
           KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM    +N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGPDRKWRVIIGSKI-NTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMWECPDFFPVA 251
           AWLG D +WRVI+GS   + RGLAI+Y+S+DF  W Q   PLHY D  GMWECPDFFPV+
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDKGSIENYNGL 311
             GS  G++T+ +V    +KHVLK+SL +T HD Y IG YD EK++YVPD G ++N +  
Sbjct: 255 ITGS-DGVETS-SVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAP 314

Query: 312 RYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTIWLDTSRKQL 371
           R DYGKYYASKTF+D   KRR+LWGWVNESS   DDI+KGWSGLQ+ PR IWLD S K+L
Sbjct: 315 RLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKEL 374

Query: 372 IQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFKKAEVLKPEW 431
           +QWPI EI+ LR  ++    KV+K GST+++ G+TAAQAD+EVSFKV+  +KA+V++P W
Sbjct: 375 LQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSW 434

Query: 432 KDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHN------KYVVLMCS 491
            DPQ +CS+   +   G+GPFGL+VLAS D++EYTSV+FRIFK ++      KYVVLMCS
Sbjct: 435 TDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCS 494

Query: 492 DQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYPTLA 551
           DQSRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHSIVES+G  G+ CIT+RVYP LA
Sbjct: 495 DQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLA 554

Query: 552 IGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           IGEN  L+VFN GT++V I  L+AWS+  AQIN
Sbjct: 555 IGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of Tan0015845 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 713.0 bits (1839), Expect = 2.7e-204
Identity = 340/536 (63.43%), Postives = 418/536 (77.99%), Query Frame = 0

Query: 44  QPYRTSYHFQPPKNWMNDPNGPMIYKGIYHLFYQYNPKGAVW--GNIVWAHSTSTDLINW 103
           QPYRT YHFQPPKNWMNDPNGPMIYKGIYHLFYQ+N  GAV      VW H+TSTDLINW
Sbjct: 40  QPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINW 99

Query: 104 VPHEHAIYPSQPSDINGCWSGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLR 163
           +    AI PS+PSDINGCWSGS TILP GKP ILYTG +  N+QVQNL  PKNL+DPYLR
Sbjct: 100 ITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLR 159

Query: 164 EWVKSPKNPLMAPTPLNHINSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDF 223
            W KSP+NPL+ P+P+NHINSS+FRDPTTAW G D +WR+  GS+   RGLAI++ SKDF
Sbjct: 160 HWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDF 219

Query: 224 IQWDQVDHPLHYGDGMGMWECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKH 283
           + W Q   PLHY DG GMWECPDFFPVA+  SR G+DT+ + SG  VKHVLK+SL DT +
Sbjct: 220 VIWKQSPKPLHYHDGTGMWECPDFFPVARTDSR-GLDTSFS-SGPMVKHVLKVSLTDTFN 279

Query: 284 DIYIIGRYDLEKEIYVPDKGSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSS 343
           D Y IG YD  +++YVPDKG +++    RYDYGK+YASKTF+D+ N+RR+LWGWVNESS 
Sbjct: 280 DYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSP 339

Query: 344 VHDDIKKGWSGLQAIPRTIWLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQ 403
             D+IKKGW+GLQAIPR +WLD S K+L+QWP+ EI++LR  ++K  NK++K GS +E+ 
Sbjct: 340 EKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVH 399

Query: 404 GITAAQADIEVSFKVRNF--KKAEVLKPEWKDPQLLCSKKGTEEGGVGPFGLLVLASKDL 463
           G+TA QAD+EV FKV  F  +KA+V++P W DPQL+CS++     G+GPFGL+VLASK++
Sbjct: 400 GVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNA-SSGLGPFGLMVLASKNM 459

Query: 464 KEYTSVFFRIFK-GHN--KYVVLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSL 523
           +EYTSV  RIF+ G N  ++VV+MCSDQS SSL   NDKTTYGAFLD+ P  + +SLR+L
Sbjct: 460 EEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QPISLRTL 519

Query: 524 IDHSIVESFGSKGKACITARVYPTLAIGENVGLYVFNNGTEAVAITKLNAWSMNKA 573
           ID SIVESFG KGK CIT+RVYP LAIGE   L+ FN G++ V +  L+AWSM  +
Sbjct: 520 IDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 571

BLAST of Tan0015845 vs. NCBI nr
Match: XP_038898203.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida])

HSP 1 Score: 1045.0 bits (2701), Expect = 2.3e-301
Identity = 494/576 (85.76%), Postives = 532/576 (92.36%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMN 60
           MASFSL FISFLC LFGHG+L+LQASHHVY+AQ S   PHHYQQPYRTSYHFQPPKNW+N
Sbjct: 1   MASFSLCFISFLCFLFGHGVLQLQASHHVYRAQLSNSLPHHYQQPYRTSYHFQPPKNWIN 60

Query: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 120
           DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+HAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120

Query: 121 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHI 180
           SGSATILPG KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 240
           NSSSFRDPTTAWLGPD +WRVIIGSKIN RGLAIMY+SKDF++WDQVDHPLHY DG GMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKINARGLAIMYQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 300
           ECPDFFPVAK G R G+DT  +VSG HVKHVLK+SLDDTKHD Y +G YDLEK+IYVP+K
Sbjct: 241 ECPDFFPVAKTG-RKGVDT--SVSGGHVKHVLKVSLDDTKHDHYTVGNYDLEKDIYVPNK 300

Query: 301 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 360
           GSIE Y GLRYDYGKYYASKTF+D Q KRRVLWGWVNESSSV DDIKKGWSG+QAIPRTI
Sbjct: 301 GSIEGYMGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRTI 360

Query: 361 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 420
           WLD S KQLIQWPI EIQKLRKDK+KLTNKV+KKGS IE++G+TAAQAD+EVSFKV+NFK
Sbjct: 361 WLDASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTAAQADVEVSFKVKNFK 420

Query: 421 KAEVLKPEWKDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVL 480
           KA++LKPEWKDPQLLCS+KG T +GGVG FGLLVLASKDLKEYTS+ F IFK   KY+VL
Sbjct: 421 KAQILKPEWKDPQLLCSQKGATTKGGVGSFGLLVLASKDLKEYTSISFTIFKRQGKYIVL 480

Query: 481 MCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYP 540
           MCSDQSRSSLN NNDKTTYGAFL+VDP+ E++SLRSLIDHS+VESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNPNNDKTTYGAFLNVDPVSEDLSLRSLIDHSVVESFGSKGKACITARVYP 540

Query: 541 TLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           TLAIG+NVGLYVFNNGTE+V ITKL AWS+ KAQIN
Sbjct: 541 TLAIGDNVGLYVFNNGTESVTITKLGAWSLKKAQIN 573

BLAST of Tan0015845 vs. NCBI nr
Match: XP_023547447.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.5e-292
Identity = 480/575 (83.48%), Postives = 523/575 (90.96%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFLC L  HG LKLQASHHVY AQAS+   HH++QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLAHGALKLQASHHVYTAQASRLLLHHHEQPYRTSYHFQPPKNWIN 60

Query: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 120
           DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHI 180
           SGSATILPG KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSKI+ RGLAIM+RSKDF++WDQVDHPLHY DGMGMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 300
           ECPDFFPVAKNG  +G+DT  +VSG HVKHVLK+SLDDTKHD Y +G YDL+K+IYVPDK
Sbjct: 241 ECPDFFPVAKNG-HAGVDT--SVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDK 300

Query: 301 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 360
           GSIE+Y GLRYDYGKYYASKTFFD Q KRRVLWGWVNESSSV DDIKKGWSG+QAIPR +
Sbjct: 301 GSIEDYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAV 360

Query: 361 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 420
           WLD S KQLIQWPI EIQKLRKDK+KLTNKV+KKGS + ++G+TAAQAD++VSFKV++F 
Sbjct: 361 WLDASGKQLIQWPIAEIQKLRKDKIKLTNKVLKKGSIVAVKGVTAAQADVDVSFKVKSFN 420

Query: 421 KAEVLKPEWKDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVL 480
           KA+ LK EWKDPQL+CS+ G   +GGVGPFGLLVLAS +L+EYTSVFFRIFKG +KYVVL
Sbjct: 421 KAQRLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKGQDKYVVL 480

Query: 481 MCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYP 540
           MCSDQSRSSLN+NNDKTTYGAFLDVDP+RENVSLRSLIDHSIVESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNANNDKTTYGAFLDVDPVRENVSLRSLIDHSIVESFGSKGKACITARVYP 540

Query: 541 TLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQI 575
            LAIG+  GLY FNNGTE+V I KL AWS+ KAQI
Sbjct: 541 RLAIGDKAGLYAFNNGTESVTIAKLRAWSLKKAQI 572

BLAST of Tan0015845 vs. NCBI nr
Match: XP_022954011.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >XP_022954012.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 1006.9 bits (2602), Expect = 7.0e-290
Identity = 479/576 (83.16%), Postives = 522/576 (90.62%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFLC L  HG LKLQASHHVY  Q S+   H Y+QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 120
           DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHI 180
           SGSATILPG KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSKI+ RGLAIM++SKDF++WDQVDHPLHY DG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 300
           ECPDFFPVAKN SR+G+DT  +VSG HVKHVLK+SLDDTKHD Y +G YDL+K+IYVPDK
Sbjct: 241 ECPDFFPVAKN-SRAGVDT--SVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDK 300

Query: 301 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 360
           GSIE Y GLRYDYGKYYASKTFFD Q KRRVLWGWVNESSSV DDIKKGWSG+QAIPR +
Sbjct: 301 GSIEGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAV 360

Query: 361 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 420
           WLD S KQLIQWPIVEIQKLRKDK+KLTNKV+KKGS I ++G+TAAQAD++VSFKV++F 
Sbjct: 361 WLDASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFN 420

Query: 421 KAEVLKPEWKDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVL 480
           KA+ LK EWKDPQL+CS+ G   +GGVGPFGLLVLAS +L+EYTSVFFRIFK  +KYVVL
Sbjct: 421 KAQPLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVL 480

Query: 481 MCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYP 540
           MCSDQSRSSLN+NNDKTTYGAFLDVDP+REN+SLRSLIDHSIVESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYP 540

Query: 541 TLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
            LAIG+  GLY FNNGTE+V ITKL+AWSM KAQI+
Sbjct: 541 RLAIGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of Tan0015845 vs. NCBI nr
Match: XP_022992217.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita maxima])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.5e-289
Identity = 478/576 (82.99%), Postives = 517/576 (89.76%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMN 60
           MAS SL FIS LC L  HG LKLQASHHVY AQAS+   HHY+QPYRTSYHFQPPKNW+N
Sbjct: 3   MASSSLCFISLLCFLLCHGALKLQASHHVYTAQASRLLLHHYEQPYRTSYHFQPPKNWIN 62

Query: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 120
           DPNGPMIYKGIYHLFYQYNPKGA WGNIVWAHSTSTDLINW PH+HAIYPSQPSDINGCW
Sbjct: 63  DPNGPMIYKGIYHLFYQYNPKGATWGNIVWAHSTSTDLINWKPHDHAIYPSQPSDINGCW 122

Query: 121 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHI 180
           SGSATILPG KPAILYTGINPKNQQVQNLA+PKNLSDPYLREWVKSP NPLMAPTP N I
Sbjct: 123 SGSATILPGEKPAILYTGINPKNQQVQNLALPKNLSDPYLREWVKSPNNPLMAPTPQNRI 182

Query: 181 NSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSKI+ RGLAIM+RSKDF++WDQVDHPLHY DG GMW
Sbjct: 183 NSSSFRDPTTAWLGHDGQWRVIIGSKIDARGLAIMFRSKDFVRWDQVDHPLHYADGTGMW 242

Query: 241 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 300
           ECPDFFPVAKNG R+GIDT  +V+G HVKHVLK+SLDDTKHD Y +G YDL+K+IYVPDK
Sbjct: 243 ECPDFFPVAKNG-RAGIDT--SVNGGHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDK 302

Query: 301 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 360
            S E Y GLRYDYGKYYASKTFFD Q KRRVLWGWVNESSSV DD+KKGWSG+QAIPR +
Sbjct: 303 ESTEGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDMKKGWSGVQAIPRAV 362

Query: 361 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 420
           WLD S KQLIQWPI EIQKLRKDK+KL NKVVKKGS I ++G+TAAQAD+EVSFKV++FK
Sbjct: 363 WLDASGKQLIQWPIAEIQKLRKDKIKLRNKVVKKGSIIAVKGVTAAQADVEVSFKVKSFK 422

Query: 421 KAEVLKPEWKDPQLLCSKKGTEE-GGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVL 480
           KA+ LK EWKDPQL CS+ G    GGVGPFGLLVLASKDL+EYTSVFFR+FKG +KYVVL
Sbjct: 423 KAQRLKAEWKDPQLACSRMGAAAMGGVGPFGLLVLASKDLEEYTSVFFRVFKGQDKYVVL 482

Query: 481 MCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYP 540
           MCSDQSRSSLN+NNDKTTYGAFLDVDP+REN+SLRSLIDHSIVESFGSKGKACITARVYP
Sbjct: 483 MCSDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYP 542

Query: 541 TLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
            LA+G+  GLY FNNGTE V ITKL+AWSM KAQI+
Sbjct: 543 KLAMGDKAGLYAFNNGTERVTITKLSAWSMKKAQIS 575

BLAST of Tan0015845 vs. NCBI nr
Match: XP_022954013.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.7e-289
Identity = 475/575 (82.61%), Postives = 523/575 (90.96%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFLC L  HG LKLQASHHVY  Q S+   HH++QPYRTS HFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 120
           DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHI 180
           SGSATILPG KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 240
           NSSSFRDPTTAWLGPD +WRVIIGSK++ RGLAIM+RSKDF++WDQVDHPLHY DGMGMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 300
           ECPDFFPVAKNG R+G+DT  +VSG HVKHVLK+SLDDTKHD Y +G YDL+K+IYVPDK
Sbjct: 241 ECPDFFPVAKNG-RAGVDT--SVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDK 300

Query: 301 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 360
           GSI++Y+GLRYDYGKYYASKTFFD Q KRRVLWGWVNESSSV DDIKKGWSG+QAIPRT+
Sbjct: 301 GSIQDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTV 360

Query: 361 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 420
           WLD S KQLIQWPI EIQKLRKDK+KLTNKV++KGS I ++G+TAAQAD++VSFKV++F 
Sbjct: 361 WLDASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFN 420

Query: 421 KAEVLKPEWKDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVL 480
           KA +LK EWKDPQL+CS+ G   +GGVGPFGLLVLAS +L+EYTSVFFRIFK  +KYVVL
Sbjct: 421 KAHLLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVL 480

Query: 481 MCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYP 540
           MCSDQSRSSLN++NDKT YGAFLDVDP+R+NVSLRSLIDHSIVESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLIDHSIVESFGSKGKACITARVYP 540

Query: 541 TLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQI 575
            LAIG+  GLY FNNGTE+V ITKL AWS+ KAQI
Sbjct: 541 RLAIGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 572

BLAST of Tan0015845 vs. ExPASy TrEMBL
Match: A0A6J1GPU7 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456399 PE=3 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.4e-290
Identity = 479/576 (83.16%), Postives = 522/576 (90.62%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFLC L  HG LKLQASHHVY  Q S+   H Y+QPYRTSYHFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 120
           DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHI 180
           SGSATILPG KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSKI+ RGLAIM++SKDF++WDQVDHPLHY DG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 300
           ECPDFFPVAKN SR+G+DT  +VSG HVKHVLK+SLDDTKHD Y +G YDL+K+IYVPDK
Sbjct: 241 ECPDFFPVAKN-SRAGVDT--SVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDK 300

Query: 301 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 360
           GSIE Y GLRYDYGKYYASKTFFD Q KRRVLWGWVNESSSV DDIKKGWSG+QAIPR +
Sbjct: 301 GSIEGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAV 360

Query: 361 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 420
           WLD S KQLIQWPIVEIQKLRKDK+KLTNKV+KKGS I ++G+TAAQAD++VSFKV++F 
Sbjct: 361 WLDASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFN 420

Query: 421 KAEVLKPEWKDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVL 480
           KA+ LK EWKDPQL+CS+ G   +GGVGPFGLLVLAS +L+EYTSVFFRIFK  +KYVVL
Sbjct: 421 KAQPLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVL 480

Query: 481 MCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYP 540
           MCSDQSRSSLN+NNDKTTYGAFLDVDP+REN+SLRSLIDHSIVESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYP 540

Query: 541 TLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
            LAIG+  GLY FNNGTE+V ITKL+AWSM KAQI+
Sbjct: 541 RLAIGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of Tan0015845 vs. ExPASy TrEMBL
Match: A0A6J1JP62 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=3661 GN=LOC111488605 PE=3 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.7e-289
Identity = 478/576 (82.99%), Postives = 517/576 (89.76%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMN 60
           MAS SL FIS LC L  HG LKLQASHHVY AQAS+   HHY+QPYRTSYHFQPPKNW+N
Sbjct: 3   MASSSLCFISLLCFLLCHGALKLQASHHVYTAQASRLLLHHYEQPYRTSYHFQPPKNWIN 62

Query: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 120
           DPNGPMIYKGIYHLFYQYNPKGA WGNIVWAHSTSTDLINW PH+HAIYPSQPSDINGCW
Sbjct: 63  DPNGPMIYKGIYHLFYQYNPKGATWGNIVWAHSTSTDLINWKPHDHAIYPSQPSDINGCW 122

Query: 121 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHI 180
           SGSATILPG KPAILYTGINPKNQQVQNLA+PKNLSDPYLREWVKSP NPLMAPTP N I
Sbjct: 123 SGSATILPGEKPAILYTGINPKNQQVQNLALPKNLSDPYLREWVKSPNNPLMAPTPQNRI 182

Query: 181 NSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 240
           NSSSFRDPTTAWLG D +WRVIIGSKI+ RGLAIM+RSKDF++WDQVDHPLHY DG GMW
Sbjct: 183 NSSSFRDPTTAWLGHDGQWRVIIGSKIDARGLAIMFRSKDFVRWDQVDHPLHYADGTGMW 242

Query: 241 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 300
           ECPDFFPVAKNG R+GIDT  +V+G HVKHVLK+SLDDTKHD Y +G YDL+K+IYVPDK
Sbjct: 243 ECPDFFPVAKNG-RAGIDT--SVNGGHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDK 302

Query: 301 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 360
            S E Y GLRYDYGKYYASKTFFD Q KRRVLWGWVNESSSV DD+KKGWSG+QAIPR +
Sbjct: 303 ESTEGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDMKKGWSGVQAIPRAV 362

Query: 361 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 420
           WLD S KQLIQWPI EIQKLRKDK+KL NKVVKKGS I ++G+TAAQAD+EVSFKV++FK
Sbjct: 363 WLDASGKQLIQWPIAEIQKLRKDKIKLRNKVVKKGSIIAVKGVTAAQADVEVSFKVKSFK 422

Query: 421 KAEVLKPEWKDPQLLCSKKGTEE-GGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVL 480
           KA+ LK EWKDPQL CS+ G    GGVGPFGLLVLASKDL+EYTSVFFR+FKG +KYVVL
Sbjct: 423 KAQRLKAEWKDPQLACSRMGAAAMGGVGPFGLLVLASKDLEEYTSVFFRVFKGQDKYVVL 482

Query: 481 MCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYP 540
           MCSDQSRSSLN+NNDKTTYGAFLDVDP+REN+SLRSLIDHSIVESFGSKGKACITARVYP
Sbjct: 483 MCSDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYP 542

Query: 541 TLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
            LA+G+  GLY FNNGTE V ITKL+AWSM KAQI+
Sbjct: 543 KLAMGDKAGLYAFNNGTERVTITKLSAWSMKKAQIS 575

BLAST of Tan0015845 vs. ExPASy TrEMBL
Match: A0A6J1GPY1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456400 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.7e-289
Identity = 475/575 (82.61%), Postives = 523/575 (90.96%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMN 60
           MAS SL FISFLC L  HG LKLQASHHVY  Q S+   HH++QPYRTS HFQPPKNW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 120
           DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH++AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHI 180
           SGSATILPG KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP NHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 240
           NSSSFRDPTTAWLGPD +WRVIIGSK++ RGLAIM+RSKDF++WDQVDHPLHY DGMGMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 300
           ECPDFFPVAKNG R+G+DT  +VSG HVKHVLK+SLDDTKHD Y +G YDL+K+IYVPDK
Sbjct: 241 ECPDFFPVAKNG-RAGVDT--SVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDK 300

Query: 301 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 360
           GSI++Y+GLRYDYGKYYASKTFFD Q KRRVLWGWVNESSSV DDIKKGWSG+QAIPRT+
Sbjct: 301 GSIQDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTV 360

Query: 361 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 420
           WLD S KQLIQWPI EIQKLRKDK+KLTNKV++KGS I ++G+TAAQAD++VSFKV++F 
Sbjct: 361 WLDASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFN 420

Query: 421 KAEVLKPEWKDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVL 480
           KA +LK EWKDPQL+CS+ G   +GGVGPFGLLVLAS +L+EYTSVFFRIFK  +KYVVL
Sbjct: 421 KAHLLKAEWKDPQLVCSQMGAAAKGGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVL 480

Query: 481 MCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYP 540
           MCSDQSRSSLN++NDKT YGAFLDVDP+R+NVSLRSLIDHSIVESFGSKGKACITARVYP
Sbjct: 481 MCSDQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLIDHSIVESFGSKGKACITARVYP 540

Query: 541 TLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQI 575
            LAIG+  GLY FNNGTE+V ITKL AWS+ KAQI
Sbjct: 541 RLAIGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 572

BLAST of Tan0015845 vs. ExPASy TrEMBL
Match: A0A6J1DM23 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia OX=3673 GN=LOC111021302 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.4e-288
Identity = 471/576 (81.77%), Postives = 522/576 (90.62%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMN 60
           MASFSLWF SFL +   HG+ KL+ASHHVY+AQ S   P  +QQPYRTSYHFQPPKNWMN
Sbjct: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60

Query: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCW 120
           DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPH+HAI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120

Query: 121 SGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHI 180
           SGSATILPG KPA+LYTGINP+N QVQNLAVPKNLSDP+L EWVKSPKNPLMAPTP N+I
Sbjct: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180

Query: 181 NSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 240
           NSSSFRDPTTAWLG DR+WRV+IGSKIN RGLAIMYRSKDFIQWDQV++PLHY D  GMW
Sbjct: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240

Query: 241 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 300
           ECPDFFPVAKN SR G+DT  +V+G  VKHVLK+SLDDTKHD Y +G Y+++K++YVPDK
Sbjct: 241 ECPDFFPVAKN-SRVGVDT--SVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDK 300

Query: 301 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 360
           GS+E Y GLRYDYGK YASKTFFD++ KRRVLWGWVNESSSV DD KKGWSG+QAIPR+I
Sbjct: 301 GSVEGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSI 360

Query: 361 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 420
           WLD S KQL+QWPIVEIQKLRKDK+KL NKV+KKGS IE++G+TAAQ D+EVSFKV+NFK
Sbjct: 361 WLDASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFK 420

Query: 421 KAEVLKPEWKDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHNKYVVL 480
           KA+VLKPEWKDPQLLC++KG  +E GVGPFGLL+LASKDLKEYTSVFF++FK   KYVVL
Sbjct: 421 KAQVLKPEWKDPQLLCAQKGAAKESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVL 480

Query: 481 MCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYP 540
           MCSDQSRSSLNSNNDKTTYGAF+D+DP+REN+SLRSLIDHSIVESFG KGKACITARVYP
Sbjct: 481 MCSDQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYP 540

Query: 541 TLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           TLAIGENVGLY FNN TE V I+KL AWS+NKA IN
Sbjct: 541 TLAIGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 573

BLAST of Tan0015845 vs. ExPASy TrEMBL
Match: A0A0A0K761 (Cell wall invertase 5 OS=Cucumis sativus OX=3659 GN=Csa_7G450770 PE=2 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 8.9e-283
Identity = 465/581 (80.03%), Postives = 521/581 (89.67%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGILKLQASHHVYKAQASQFFPHHY--QQPYRTSYHFQPPKNW 60
           MAS SL FISFLC LFGHG+ +LQASHHV+K Q S   P+HY  +QPYRTSYHFQPPKNW
Sbjct: 1   MASISLCFISFLCFLFGHGVFQLQASHHVHKPQTSTSLPYHYHHRQPYRTSYHFQPPKNW 60

Query: 61  MNDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDING 120
           +NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH+HAI+PSQPSDING
Sbjct: 61  INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDING 120

Query: 121 CWSGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLN 180
           CWSGSATILPG KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP N
Sbjct: 121 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 180

Query: 181 HINSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMG 240
            IN+SSFRDPTTAWLGP+ +WRVI+G+K++TRGLA+M++SKDFI+WDQVDHPLHY DG G
Sbjct: 181 QINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPLHYADGSG 240

Query: 241 MWECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVP 300
           MWECPDF+PVAK G R G+DT  TV+G +VKHVLK+SLDDT+HD+Y IG YD+EK+IYVP
Sbjct: 241 MWECPDFYPVAKTG-RRGVDT--TVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKDIYVP 300

Query: 301 DKGSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPR 360
           +KGSIE Y+GLRYDYGKYYASKTF+D Q KRRVLWGWVNESSSV DDIKKGWSG+Q IPR
Sbjct: 301 NKGSIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQGIPR 360

Query: 361 TIWLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRN 420
           TIWLD S KQLIQWPI EIQKLRK+K+ LTNKV+KKGSTIE++G+T +QAD+EVSFKV+N
Sbjct: 361 TIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKN 420

Query: 421 FKKAEVLKPE-WKDPQLLCSKKG---TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHN 480
            KKA++LKP+ W++PQ+LCS+KG   T +GGVGPFGLLVLAS D KEYTSV F IFK  +
Sbjct: 421 LKKAQILKPQYWENPQVLCSQKGRAATTKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQS 480

Query: 481 KYVVLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACIT 540
           KYV LMCSDQSRSSLN  NDKTTYGAFLDVD   E++SLRSLIDHS+VESFG KGK CIT
Sbjct: 481 KYVALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKGKGCIT 540

Query: 541 ARVYPTLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           ARVYPTLAIG+NVGLY FNNGTE+V ITKL AWSM KA+IN
Sbjct: 541 ARVYPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARIN 578

BLAST of Tan0015845 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 808.1 bits (2086), Expect = 4.4e-234
Identity = 387/581 (66.61%), Postives = 462/581 (79.52%), Query Frame = 0

Query: 2   ASFSLWFISFLCLLFGHGILKLQASHHVYK--AQASQFFPHHYQQPYRTSYHFQPPKNWM 61
           ++  LW +  L LL G+ ++ L+ASHHVYK   Q++        QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGC 121
           NDPNGPMIYKGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNH 181
           WSGSATILP GKP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP  +N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGM 241
           IN+SSFRDPTTAWLG D+KWRVIIGSKI+ RGLAI Y SKDF++W++   PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPD 301
           WECPDFFPV + GS     ++       +KHVLK+SLDDTKHD Y IG YD  K+ +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRT 361
            G   +    RYDYGKYYASKTFFD+   RR+LWGW NESSSV DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 IWLDTSRKQLIQWPIVEIQKLRKDKLK-LTNKVVKKGSTIEIQGITAAQADIEVSFKVRN 421
           IWLD S KQLIQWP+ E+++LR  ++K L NKV+K GS +E+ G+TAAQAD+EV FKVR+
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FKKAEVLKPEWKDPQLLCSKKGTE-EGGVGPFGLLVLASKDLKEYTSVFFRIFKGH---N 481
            +KA+V++P W DPQL+CSK     + G+GPFGL+VLASK+L+EYTSV+FRIFK     N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACIT 541
           KYVVLMCSDQSRSSL  +NDKTTYGAF+D++P  + +SLR+LIDHS+VESFG KG+ACIT
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACIT 546

Query: 542 ARVYPTLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           +RVYP LAIG++  L+ FN G ++V +  LNAWSMN AQI+
Sbjct: 547 SRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of Tan0015845 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 795.8 bits (2054), Expect = 2.3e-230
Identity = 384/581 (66.09%), Postives = 459/581 (79.00%), Query Frame = 0

Query: 2   ASFSLWFISFLCLLFGHGILKLQASHHVYK--AQASQFFPHHYQQPYRTSYHFQPPKNWM 61
           ++  LW +  L LL G+ ++ L+ASHHVYK   Q++        QPYRT +HFQPPKNWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  NDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHEHAIYPSQPSDINGC 121
              NGPMIYKGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW PH  AI+PS P DINGC
Sbjct: 67  ---NGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNH 181
           WSGSATILP GKP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP  +N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGM 241
           IN+SSFRDPTTAWLG D+KWRVIIGSKI+ RGLAI Y SKDF++W++   PLHY DG GM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPD 301
           WECPDFFPV + GS     ++       +KHVLK+SLDDTKHD Y IG YD  K+ +VPD
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KGSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRT 361
            G   +    RYDYGKYYASKTFFD+   RR+LWGW NESSSV DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 IWLDTSRKQLIQWPIVEIQKLRKDKLK-LTNKVVKKGSTIEIQGITAAQADIEVSFKVRN 421
           IWLD S KQLIQWP+ E+++LR  ++K L NKV+K GS +E+ G+TAAQAD+EV FKVR+
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FKKAEVLKPEWKDPQLLCSKKGTE-EGGVGPFGLLVLASKDLKEYTSVFFRIFKGH---N 481
            +KA+V++P W DPQL+CSK     + G+GPFGL+VLASK+L+EYTSV+FRIFK     N
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSN 486

Query: 482 KYVVLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACIT 541
           KYVVLMCSDQSRSSL  +NDKTTYGAF+D++P  + +SLR+LIDHS+VESFG KG+ACIT
Sbjct: 487 KYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACIT 546

Query: 542 ARVYPTLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           +RVYP LAIG++  L+ FN G ++V +  LNAWSMN AQI+
Sbjct: 547 SRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581

BLAST of Tan0015845 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 725.3 bits (1871), Expect = 3.8e-209
Identity = 351/573 (61.26%), Postives = 425/573 (74.17%), Query Frame = 0

Query: 12  LCLLFGHGILKLQASHHVYKAQASQFFPHHYQQPYRTSYHFQPPKNWMNDPNGPMIYKGI 71
           L +   + I  L+AS H               QPYRT YHFQP KNWMNDPNGPMIYKGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCWSGSATILPGG 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWSGS TILP G
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHINSSSFRDPTT 191
           KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM    +N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGPDRKWRVIIGSKI-NTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMWECPDFFPVA 251
           AWLG D +WRVI+GS   + RGLAI+Y+S+DF  W Q   PLHY D  GMWECPDFFPV+
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDKGSIENYNGL 311
             GS  G++T+ +V    +KHVLK+SL +T HD Y IG YD EK++YVPD G ++N +  
Sbjct: 255 ITGS-DGVETS-SVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAP 314

Query: 312 RYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTIWLDTSRKQL 371
           R DYGKYYASKTF+D   KRR+LWGWVNESS   DDI+KGWSGLQ+ PR IWLD S K+L
Sbjct: 315 RLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKEL 374

Query: 372 IQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFKKAEVLKPEW 431
           +QWPI EI+ LR  ++    KV+K GST+++ G+TAAQAD+EVSFKV+  +KA+V++P W
Sbjct: 375 LQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSW 434

Query: 432 KDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHN------KYVVLMCS 491
            DPQ +CS+   +   G+GPFGL+VLAS D++EYTSV+FRIFK ++      KYVVLMCS
Sbjct: 435 TDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCS 494

Query: 492 DQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACITARVYPTLA 551
           DQSRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHSIVES+G  G+ CIT+RVYP LA
Sbjct: 495 DQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLA 554

Query: 552 IGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           IGEN  L+VFN GT++V I  L+AWS+  AQIN
Sbjct: 555 IGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of Tan0015845 vs. TAIR 10
Match: AT3G13784.1 (cell wall invertase 5 )

HSP 1 Score: 700.3 bits (1806), Expect = 1.3e-201
Identity = 337/536 (62.87%), Postives = 415/536 (77.43%), Query Frame = 0

Query: 44  QPYRTSYHFQPPKNWMNDPNGPMIYKGIYHLFYQYNPKGAVW--GNIVWAHSTSTDLINW 103
           QPYRT YHFQPPKNWM   NGPMIYKGIYHLFYQ+N  GAV      VW H+TSTDLINW
Sbjct: 40  QPYRTGYHFQPPKNWM---NGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINW 99

Query: 104 VPHEHAIYPSQPSDINGCWSGSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLR 163
           +    AI PS+PSDINGCWSGS TILP GKP ILYTG +  N+QVQNL  PKNL+DPYLR
Sbjct: 100 ITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLR 159

Query: 164 EWVKSPKNPLMAPTPLNHINSSSFRDPTTAWLGPDRKWRVIIGSKINTRGLAIMYRSKDF 223
            W KSP+NPL+ P+P+NHINSS+FRDPTTAW G D +WR+  GS+   RGLAI++ SKDF
Sbjct: 160 HWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDF 219

Query: 224 IQWDQVDHPLHYGDGMGMWECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKH 283
           + W Q   PLHY DG GMWECPDFFPVA+  SR G+DT+ + SG  VKHVLK+SL DT +
Sbjct: 220 VIWKQSPKPLHYHDGTGMWECPDFFPVARTDSR-GLDTSFS-SGPMVKHVLKVSLTDTFN 279

Query: 284 DIYIIGRYDLEKEIYVPDKGSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSS 343
           D Y IG YD  +++YVPDKG +++    RYDYGK+YASKTF+D+ N+RR+LWGWVNESS 
Sbjct: 280 DYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSP 339

Query: 344 VHDDIKKGWSGLQAIPRTIWLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQ 403
             D+IKKGW+GLQAIPR +WLD S K+L+QWP+ EI++LR  ++K  NK++K GS +E+ 
Sbjct: 340 EKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVH 399

Query: 404 GITAAQADIEVSFKVRNF--KKAEVLKPEWKDPQLLCSKKGTEEGGVGPFGLLVLASKDL 463
           G+TA QAD+EV FKV  F  +KA+V++P W DPQL+CS++     G+GPFGL+VLASK++
Sbjct: 400 GVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNA-SSGLGPFGLMVLASKNM 459

Query: 464 KEYTSVFFRIFK-GHN--KYVVLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSL 523
           +EYTSV  RIF+ G N  ++VV+MCSDQS SSL   NDKTTYGAFLD+ P  + +SLR+L
Sbjct: 460 EEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QPISLRTL 519

Query: 524 IDHSIVESFGSKGKACITARVYPTLAIGENVGLYVFNNGTEAVAITKLNAWSMNKA 573
           ID SIVESFG KGK CIT+RVYP LAIGE   L+ FN G++ V +  L+AWSM  +
Sbjct: 520 IDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568

BLAST of Tan0015845 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 686.4 bits (1770), Expect = 2.0e-197
Identity = 327/522 (62.64%), Postives = 399/522 (76.44%), Query Frame = 0

Query: 63  NGPMIYKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWVPHEHAIYPSQPSDINGCWS 122
           +GPMIYKGIYHLFYQYNP GAVW   IVW HSTS DL+NW+    A  PSQPSDINGCWS
Sbjct: 40  SGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWS 99

Query: 123 GSATILPGGKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPLNHIN 182
           GS TILP GKP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM    +N IN
Sbjct: 100 GSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGIN 159

Query: 183 SSSFRDPTTAWLGPDRKWRVIIGSKI-NTRGLAIMYRSKDFIQWDQVDHPLHYGDGMGMW 242
              FRDPTTAWLG D +WRVI+GS   + RGLAI+Y+S+DF  W Q   PLHY D  GMW
Sbjct: 160 PDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMW 219

Query: 243 ECPDFFPVAKNGSRSGIDTTVTVSGWHVKHVLKMSLDDTKHDIYIIGRYDLEKEIYVPDK 302
           ECPDFFPV+  GS  G++T+ +V    +KHVLK+SL +T HD Y IG YD EK++YVPD 
Sbjct: 220 ECPDFFPVSITGS-DGVETS-SVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDL 279

Query: 303 GSIENYNGLRYDYGKYYASKTFFDTQNKRRVLWGWVNESSSVHDDIKKGWSGLQAIPRTI 362
           G ++N +  R DYGKYYASKTF+D   KRR+LWGWVNESS   DDI+KGWSGLQ+ PR I
Sbjct: 280 GFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKI 339

Query: 363 WLDTSRKQLIQWPIVEIQKLRKDKLKLTNKVVKKGSTIEIQGITAAQADIEVSFKVRNFK 422
           WLD S K+L+QWPI EI+ LR  ++    KV+K GST+++ G+TAAQAD+EVSFKV+  +
Sbjct: 340 WLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELE 399

Query: 423 KAEVLKPEWKDPQLLCSKKG-TEEGGVGPFGLLVLASKDLKEYTSVFFRIFKGHN----- 482
           KA+V++P W DPQ +CS+   +   G+GPFGL+VLAS D++EYTSV+FRIFK ++     
Sbjct: 400 KADVIEPSWTDPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKK 459

Query: 483 -KYVVLMCSDQSRSSLNSNNDKTTYGAFLDVDPLRENVSLRSLIDHSIVESFGSKGKACI 542
            KYVVLMCSDQSRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHSIVES+G  G+ CI
Sbjct: 460 TKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCI 519

Query: 543 TARVYPTLAIGENVGLYVFNNGTEAVAITKLNAWSMNKAQIN 576
           T+RVYP LAIGEN  L+VFN GT++V I  L+AWS+  AQIN
Sbjct: 520 TSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q438666.3e-23366.61Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3... [more]
Q430892.7e-21263.78Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 P... [more]
Q70XE64.4e-21062.63Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1[more]
Q67XZ35.3e-20861.26Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3... [more]
Q9LIB92.7e-20463.43Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_038898203.12.3e-30185.76beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida][more]
XP_023547447.17.5e-29283.48beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. ... [more]
XP_022954011.17.0e-29083.16beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >X... [more]
XP_022992217.13.5e-28982.99beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita maxima][more]
XP_022954013.17.7e-28982.61beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1GPU73.4e-29083.16beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1JP621.7e-28982.99beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=... [more]
A0A6J1GPY13.7e-28982.61beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata O... [more]
A0A6J1DM235.4e-28881.77beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia ... [more]
A0A0A0K7618.9e-28380.03Cell wall invertase 5 OS=Cucumis sativus OX=3659 GN=Csa_7G450770 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13790.14.4e-23466.61Glycosyl hydrolases family 32 protein [more]
AT3G13790.22.3e-23066.09Glycosyl hydrolases family 32 protein [more]
AT1G55120.13.8e-20961.26beta-fructofuranosidase 5 [more]
AT3G13784.11.3e-20162.87cell wall invertase 5 [more]
AT1G55120.32.0e-19762.64beta-fructofuranosidase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 51..529
e-value: 9.7E-248
score: 839.0
NoneNo IPR availableGENE3D2.60.120.560coord: 385..569
e-value: 1.2E-80
score: 271.7
NoneNo IPR availablePANTHERPTHR31953:SF51CELL-WALL INVERTASEcoord: 7..575
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 7..575
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 57..362
e-value: 1.71083E-167
score: 475.723
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 51..373
e-value: 3.9E-108
score: 361.6
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 376..568
e-value: 1.1E-30
score: 106.8
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 55..384
e-value: 5.1E-119
score: 399.6
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 41..374
IPR018053Glycoside hydrolase, family 32, active sitePROSITEPS00609GLYCOSYL_HYDROL_F32coord: 51..64
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 373..571

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0015845.1Tan0015845.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds