Tan0015572 (gene) Snake gourd v1

BLAST of Tan0015572 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 4.2e-115
Identity = 215/356 (60.39%), Postives = 261/356 (73.31%), Query Frame = 0

Query: 16  EMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLSQLVSAIQLHPNKTHF 75
           E++ A+AHIWN IFNFINSMSLKCA++LGIPD I+ HG  PM +SQL  A+ ++  K+  
Sbjct: 25  EILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHG-KPMTISQLTLALPINRKKSPC 84

Query: 76  IYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKHNPCA----------- 135
           +YRLMRIL HSGFF LQK     E+   EEEGYV+T++S+LLLK NP +           
Sbjct: 85  VYRLMRILIHSGFFALQK-AEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLDP 144

Query: 136 -------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFNAGMASDARLVMSVLLE 195
                  FLS W +  D TPF+TA+G+ FW+Y   + K    FN  MASDARLV SV++E
Sbjct: 145 VMTKPWDFLSNWFQNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLVTSVVIE 204

Query: 196 KYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVADLKGERNLKYVQGDM 255
           K KGVF+G+ SLVDVGGGTGT+A +IA AFP IQCTVFDLP VVADL+G  NL +V GDM
Sbjct: 205 KCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGGNNLNFVGGDM 264

Query: 256 FEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGKVMVIDRVVGNKKNKD 315
           F  +P+   +LLKWILHDW+DEESVKILKKCK AI S  N   GKV++ID  V N+K++D
Sbjct: 265 FVDVPATEVVLLKWILHDWNDEESVKILKKCKEAI-SKSNKKGGKVIIIDMKVENEKDED 324

Query: 316 D-SIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIMGLRSLIEIYP 353
           D S ETQLFFDMLMM L TGRER E+EWA+L K+AGFS Y I PI+GLRSLIE+YP
Sbjct: 325 DESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGLRSLIEVYP 377

BLAST of Tan0015572 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 397.1 bits (1019), Expect = 2.0e-109
Identity = 201/359 (55.99%), Postives = 249/359 (69.36%), Query Frame = 0

Query: 12  VAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLSQLVSAIQLHPN 71
           V   E++ A+AH+WNHIFNFI SMSLKCA++LGIPD IH HG  PM L +LV+ + +HP 
Sbjct: 7   VISAELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPELVAKLPVHPK 66

Query: 72  KTHFIYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKHN---------- 131
           ++  +YRLMRIL HSGF   Q++     QQ +EEEGYVLT++SRLLL  +          
Sbjct: 67  RSQCVYRLMRILVHSGFLAAQRV-----QQGKEEEGYVLTDASRLLLMDDSLSIRPLVLA 126

Query: 132 --------PCAFLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFNAGMASDARLVMS 191
                   P  +LS W + DD TPF TA+   FW+Y G + +  + FN  MASDARL+ S
Sbjct: 127 MLDPILTKPWHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTS 186

Query: 192 VLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVADLKGERNLKYV 251
           VLL++ +GVF GLNSLVDVGGGTG +AK IA AFP + CTV DL  VVA L+G +NL Y 
Sbjct: 187 VLLKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSKNLNYF 246

Query: 252 QGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGKVMVIDRVVGNK 311
            GDMFEAIP   A+LLKWILHDWS+EE VKILK+C+ AI S  NG  GKV++ID ++   
Sbjct: 247 AGDMFEAIPPADAILLKWILHDWSNEECVKILKRCREAIPSKENG--GKVIIIDMIMMKN 306

Query: 312 KNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIMGLRSLIEIYP 353
           +    S ETQLFFDM MMI   GRER+E EW +L  +AGFS Y I PI+GLRSLIE+YP
Sbjct: 307 QGDYKSTETQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

BLAST of Tan0015572 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 8.6e-100
Identity = 180/360 (50.00%), Postives = 245/360 (68.06%), Query Frame = 0

Query: 13  AGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLSQLVSAIQLHPNK 72
           +G+E++ A+AHI+NHIFNFI+SM+LKCAVEL IP+ +H H P  + LS+LV A+Q+   K
Sbjct: 20  SGEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTLSELVQALQIPQAK 79

Query: 73  THFIYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKHNPCA-------- 132
           +  +YRL+RIL HSGFF + K+ SE      +EEGY+ T SS+LLLK++P +        
Sbjct: 80  SACLYRLLRILVHSGFFAITKIQSE-----GDEEGYLPTLSSKLLLKNHPMSMSPCLLGL 139

Query: 133 ----------FLSTWLK-TDDQTPFETAHGLPFWEYLGKKSKDGDVFNAGMASDARLVMS 192
                     F S W K +DD TPFE  HG   W+Y G+     ++FN  M  + RL MS
Sbjct: 140 VNPTMVAPMHFFSDWFKRSDDMTPFEATHGASLWKYFGETPHMAEIFNEAMGCETRLAMS 199

Query: 193 VLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVADLKGERNLKYV 252
           V+L++ KG  +G++SLVDVGGGTG + + IA AFP ++CTV DLPQVV +LKG  NL++V
Sbjct: 200 VVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGSNNLEFV 259

Query: 253 QGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGKVMVIDRVVG-N 312
            GDMF+ IP    + LKWILHDW+DEE +KILK+CK AI S   G  GK+++ID VV  +
Sbjct: 260 SGDMFQFIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEG--GKLIIIDMVVNDH 319

Query: 313 KKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIMGLRSLIEIYP 353
            K   +S ETQLF+D+ +M L TG ER E EW +L   AGF+ Y I P++GL+S+IE++P
Sbjct: 320 NKGSYESTETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLGLKSIIEVFP 372

BLAST of Tan0015572 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 4.9e-95
Identity = 176/362 (48.62%), Postives = 244/362 (67.40%), Query Frame = 0

Query: 11  IVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLSQLVSAIQLHP 70
           I +  E++EA+AH+WNHI+++INSMSLKCA++LGIPDAIH+HG +P+ LSQL  A+ ++ 
Sbjct: 7   ISSKQELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHG-NPITLSQLADALNINK 66

Query: 71  NKTHFIYRLMRILTHSGFF--VLQKLISEKEQQHEEEEGYVLTNSSRLLLKHNPCA---- 130
            K+H ++RLMRIL HSGFF  V  K+  E E + EEE+ Y LT +SRLLL+  P +    
Sbjct: 67  AKSHGLFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAPF 126

Query: 131 --------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFNAGMASDARL 190
                          LS W + D    F+T +G+ F EY     +   +FN  MA DA  
Sbjct: 127 ALAMSDPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGF 186

Query: 191 VMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVADLKGERNL 250
           V S+L  + + +FDGL S+VDVGGGTG  AK IA AFP ++CTV DLP VV  LKG  NL
Sbjct: 187 VNSILTTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGSENL 246

Query: 251 KYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGKVMVIDRVV 310
            +V GDMF+ IP   A+ +K+ILHDW+DEE VKILKKCK AI S  N +  K+++++ V+
Sbjct: 247 SFVSGDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAI-SRSNNSCRKIILVEIVM 306

Query: 311 GNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIMGLRSLIEI 353
            ++K   ++ ET+LFFDM M+ + TG+ER E+EW +L  +AGF+ Y I  ++GLRS+IE+
Sbjct: 307 EDEKETHEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITRVLGLRSVIEV 366

BLAST of Tan0015572 vs. ExPASy Swiss-Prot
Match: Q8GSN1 (Myricetin O-methyltransferase OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 6.8e-89
Identity = 170/357 (47.62%), Postives = 236/357 (66.11%), Query Frame = 0

Query: 16  EMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLSQLVSAIQLHPNKTHF 75
           E+  A+AH +  +F+F +  SLKCAV+LGIPDAIH HG  PM LS L +++ ++P+K  +
Sbjct: 7   EIRNAQAHFFTQVFSFTSMSSLKCAVQLGIPDAIHSHG-KPMALSDLTNSLPINPSKAPY 66

Query: 76  IYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKHNPC------------ 135
           IYRLMRIL  +G+F             EE+  Y LT  +RLLLK++P             
Sbjct: 67  IYRLMRILVAAGYF-----------SEEEKNVYSLTPFTRLLLKNDPLNSISMVLGVNQI 126

Query: 136 ------AFLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFNAGMASDARLVMSVLLE 195
                   +S W + +D T FETAHG  FW++ G + K G  F+  MA+D+ LV  +L+ 
Sbjct: 127 AELKAWNAMSEWFQNEDLTAFETAHGKNFWDF-GAEDKYGKNFDGVMAADSILVSKMLIP 186

Query: 196 KYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVADLKGERNLKYVQGDM 255
           ++  +F+GL+SLVDVGGGTGT+AK IA++FP ++CTVFDLP VVA+L+   NL++V GDM
Sbjct: 187 EFNYLFEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLESTENLEFVGGDM 246

Query: 256 FEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGKVMVIDRVV--GNKKN 315
           FE IPS +A+LLKWILHDW DEE VK+LK C+ AI     G  GKV++I+ V+    K  
Sbjct: 247 FEKIPSANAILLKWILHDWKDEECVKVLKMCRKAIPEKEKG--GKVILIETVLMDSKKHE 306

Query: 316 KDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIMGLRSLIEIYP 353
            +++++ Q+  D+ MM+  T +ER EEEWA L +EAGFSGY IFP++  RS IE+YP
Sbjct: 307 NEEAVKAQISSDIDMMVFFTAKERTEEEWATLFREAGFSGYKIFPMIDFRSPIEVYP 348

BLAST of Tan0015572 vs. NCBI nr
Match: KAG6572273.1 (Trans-resveratrol di-O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 547.7 bits (1410), Expect = 7.1e-152
Identity = 274/372 (73.66%), Postives = 311/372 (83.60%), Query Frame = 0

Query: 1   MNLDECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLS 60
           MNL E   MA +AG+E++EA+AHIWNH+FNFINSM+LKCA++LGIPDAIHRHGP+PMPLS
Sbjct: 1   MNL-EGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPNPMPLS 60

Query: 61  QLVSAIQLHPNKTHFIYRLMRILTHSGFFVLQKL--ISEKEQQHEEEEGYVLTNSSRLLL 120
           +LVSA+QLHPNKT FIYRLMR+LTHSGFFV QKL   S  E++ EEEEGYVLTNSSRLLL
Sbjct: 61  RLVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEEGYVLTNSSRLLL 120

Query: 121 KHNPCA------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVF 180
           KHNPC                    LSTW +++D+TPF TAHG+P WE+LG K KDG+ F
Sbjct: 121 KHNPCGVTPFLLSMLQPALVEPWQILSTWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGF 180

Query: 181 NAGMASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQV 240
           N GMASDARLVMSVLL+K+KGVFDG+ SLVDVGGGTGT+AK IA+AFPQI+CTV DLPQV
Sbjct: 181 NEGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAQAFPQIECTVLDLPQV 240

Query: 241 VADLKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNT 300
           VA+LKGE NLKYV+GDMF+AIPS  ALLLKWILHDWSDEE VKILK CKAAITS  NG  
Sbjct: 241 VAELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDEECVKILKNCKAAITS--NGYK 300

Query: 301 GKVMVIDRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFP 353
           GKVMVID VVG K  + DS ETQLF+DMLMMIL  G+EREE+EWA+LIKEAGFSGYNI P
Sbjct: 301 GKVMVIDMVVGYKGKESDSYETQLFYDMLMMILVGGKEREEKEWAKLIKEAGFSGYNILP 360

BLAST of Tan0015572 vs. NCBI nr
Match: XP_022952337.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 546.6 bits (1407), Expect = 1.6e-151
Identity = 271/371 (73.05%), Postives = 309/371 (83.29%), Query Frame = 0

Query: 1   MNLDECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLS 60
           MNL E   MA +AG+E++EA+AHIWNH+FNFINSM+LKCA++LGIPDAIHRHGPHPMPLS
Sbjct: 1   MNL-EGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPHPMPLS 60

Query: 61  QLVSAIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEKE-QQHEEEEGYVLTNSSRLLLK 120
           +LVSA+QLHPNKT FIYRLMR+LTHSGFFV QKL      ++ EEEEGYVLTNSSRLLLK
Sbjct: 61  RLVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEGYVLTNSSRLLLK 120

Query: 121 HNPCA------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFN 180
           HNPCA                   LSTW +++D+TPF TAHG+PFWE+LG K KDG+ FN
Sbjct: 121 HNPCAVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFN 180

Query: 181 AGMASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVV 240
           AGMASDARLVMSVLL+K+KGVFDG+ SLVDVGGGTGT+ K IA AFPQI+CTVFDLPQVV
Sbjct: 181 AGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECTVFDLPQVV 240

Query: 241 ADLKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTG 300
            +LKGE NLKYV+GDMF+AIP   ALLLKWILHDWSD+E VKILK CKAAITS  NG  G
Sbjct: 241 GELKGEGNLKYVEGDMFKAIPPADALLLKWILHDWSDQECVKILKNCKAAITS--NGYKG 300

Query: 301 KVMVIDRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPI 353
           KVMVID VVG K  + DS ETQLF++MLMMIL  G+EREE+EWA+LI+EAGFSGYNI PI
Sbjct: 301 KVMVIDMVVGYKGKESDSYETQLFYEMLMMILVGGKEREEKEWAKLIEEAGFSGYNILPI 360

BLAST of Tan0015572 vs. NCBI nr
Match: XP_023554523.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540.4 bits (1391), Expect = 1.1e-149
Identity = 268/370 (72.43%), Postives = 306/370 (82.70%), Query Frame = 0

Query: 1   MNLDECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLS 60
           MNL E   MA +AG+E++EA+AHIWNH+FNFINSM+LKCA++LGIPDAIHRHGP+PMPLS
Sbjct: 1   MNL-EGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPNPMPLS 60

Query: 61  QLVSAIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKH 120
           +LVSA+QLH NKT FIYRLMR+LTHSGFFV QKL      + EEEEGYVLTNSSRLLLKH
Sbjct: 61  RLVSALQLHSNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEGYVLTNSSRLLLKH 120

Query: 121 NPCA------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFNA 180
           NPC                    LSTW +++D+TPF TAHG+P WE+LG K KDG+ FN 
Sbjct: 121 NPCGVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGFNE 180

Query: 181 GMASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVA 240
           GMASDARLVMSVLL+K+K VFDG+ SLVDVGGGTGT+AK IA+AFPQI+CTV DLPQVVA
Sbjct: 181 GMASDARLVMSVLLDKHKAVFDGVASLVDVGGGTGTVAKAIAQAFPQIECTVLDLPQVVA 240

Query: 241 DLKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGK 300
           +LKGE NLKYV+GDMF+AIPS  ALLLKWILHDWSDEE V+ILK CKAAITS  NG  GK
Sbjct: 241 ELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDEECVRILKNCKAAITS--NGYKGK 300

Query: 301 VMVIDRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIM 353
           VMVID VVG K  + DS ETQLF+DMLMMIL  G+EREE+EWA+LI+EAGFSGYNI PI+
Sbjct: 301 VMVIDMVVGYKGKESDSYETQLFYDMLMMILVGGKEREEKEWAKLIEEAGFSGYNILPIV 360

BLAST of Tan0015572 vs. NCBI nr
Match: XP_022969140.1 (trans-resveratrol di-O-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 536.6 bits (1381), Expect = 1.6e-148
Identity = 266/371 (71.70%), Postives = 306/371 (82.48%), Query Frame = 0

Query: 1   MNLDECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLS 60
           MNL E   MA +AG+E++EA+AHIWNH+FNFINSM+LKCA++LGIPDAIHRH PHPMPLS
Sbjct: 1   MNL-EGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHEPHPMPLS 60

Query: 61  QLVSAIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEK-EQQHEEEEGYVLTNSSRLLLK 120
           +LVSA+QLHPNKT FIYRLMR+LTHSGFFV QK+     +++ EEEEGYVLTNSSRLLLK
Sbjct: 61  RLVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKVPKNSGKEEEEEEEGYVLTNSSRLLLK 120

Query: 121 HNPCA------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFN 180
           HNPC                    LS W +++D+TPF TAHG+P WE+LG K KDG+ FN
Sbjct: 121 HNPCGATPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGFN 180

Query: 181 AGMASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVV 240
           AGMASDARLVMSVLL+K+KGVFDG+ SLVDVGGGTGT+AK IA AFPQI+CTV DLPQVV
Sbjct: 181 AGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVLDLPQVV 240

Query: 241 ADLKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTG 300
            +LKGE NLKYV+GDMF+AIPS  ALLLKWILHDWSD E V+ILK CKAAITS  +G  G
Sbjct: 241 GELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDPECVRILKNCKAAITS--HGYKG 300

Query: 301 KVMVIDRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPI 353
           KVMVID VVG K  + DS ETQLF+DMLMMIL  G+EREE+EWA+LI+EAGFSGYNI PI
Sbjct: 301 KVMVIDMVVGYKGKESDSYETQLFYDMLMMILVGGKEREEKEWAKLIREAGFSGYNILPI 360

BLAST of Tan0015572 vs. NCBI nr
Match: XP_023554524.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.0 bits (1377), Expect = 4.8e-148
Identity = 264/366 (72.13%), Postives = 297/366 (81.15%), Query Frame = 0

Query: 5   ECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLSQLVS 64
           E E M I+AGDEM+EA+AHIWNH+FNFINSM+LKCAVELGIPDAIHRH PHPMPLS LVS
Sbjct: 4   EGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDAIHRHAPHPMPLSHLVS 63

Query: 65  AIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKHNPCA 124
           A+QLH NKT FIYRLMR+LTHSGFFVLQKL     +Q +EE+GYVLTNSSRLLLKHNPCA
Sbjct: 64  ALQLHSNKTQFIYRLMRLLTHSGFFVLQKLPKNNGKQ-QEEDGYVLTNSSRLLLKHNPCA 123

Query: 125 ------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFNAGMAS 184
                              LSTW ++ D TPF TAHG+PFWE+LG K KDG+ FNAGMAS
Sbjct: 124 VTPFLLSMLQPALVEPWQLLSTWFQSQDPTPFHTAHGMPFWEFLGSKVKDGEGFNAGMAS 183

Query: 185 DARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVADLKG 244
           DARLVMSVLL+K+KGVFDG+ SLVDVGGGTGT+AK IA AFPQI+CTVFDLPQVV +LKG
Sbjct: 184 DARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAHAFPQIECTVFDLPQVVGELKG 243

Query: 245 ERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGKVMVI 304
           E NLKYV+GDMF AIP    L+LKWILHDW+DEE VKILKKCK AI S  NG  GKV+VI
Sbjct: 244 EGNLKYVEGDMFNAIPPADVLVLKWILHDWNDEECVKILKKCKEAIRS--NGKKGKVIVI 303

Query: 305 DRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIMGLRS 353
           D VVGN K  D  I+ QLFFDMLMM L +G+ER+E+EW++L KEAGF GY IFPI+G RS
Sbjct: 304 DMVVGNNKRDDTFIQAQLFFDMLMMGLVSGKERDEKEWSELFKEAGFGGYKIFPILGSRS 363

BLAST of Tan0015572 vs. ExPASy TrEMBL
Match: A0A6J1GK49 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455045 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 7.7e-152
Identity = 271/371 (73.05%), Postives = 309/371 (83.29%), Query Frame = 0

Query: 1   MNLDECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLS 60
           MNL E   MA +AG+E++EA+AHIWNH+FNFINSM+LKCA++LGIPDAIHRHGPHPMPLS
Sbjct: 1   MNL-EGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHGPHPMPLS 60

Query: 61  QLVSAIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEKE-QQHEEEEGYVLTNSSRLLLK 120
           +LVSA+QLHPNKT FIYRLMR+LTHSGFFV QKL      ++ EEEEGYVLTNSSRLLLK
Sbjct: 61  RLVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKLPKNSGLEEEEEEEGYVLTNSSRLLLK 120

Query: 121 HNPCA------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFN 180
           HNPCA                   LSTW +++D+TPF TAHG+PFWE+LG K KDG+ FN
Sbjct: 121 HNPCAVTPFLLSMLQPALVEPWQLLSTWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFN 180

Query: 181 AGMASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVV 240
           AGMASDARLVMSVLL+K+KGVFDG+ SLVDVGGGTGT+ K IA AFPQI+CTVFDLPQVV
Sbjct: 181 AGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECTVFDLPQVV 240

Query: 241 ADLKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTG 300
            +LKGE NLKYV+GDMF+AIP   ALLLKWILHDWSD+E VKILK CKAAITS  NG  G
Sbjct: 241 GELKGEGNLKYVEGDMFKAIPPADALLLKWILHDWSDQECVKILKNCKAAITS--NGYKG 300

Query: 301 KVMVIDRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPI 353
           KVMVID VVG K  + DS ETQLF++MLMMIL  G+EREE+EWA+LI+EAGFSGYNI PI
Sbjct: 301 KVMVIDMVVGYKGKESDSYETQLFYEMLMMILVGGKEREEKEWAKLIEEAGFSGYNILPI 360

BLAST of Tan0015572 vs. ExPASy TrEMBL
Match: A0A6J1HZ45 (trans-resveratrol di-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111468224 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 7.9e-149
Identity = 266/371 (71.70%), Postives = 306/371 (82.48%), Query Frame = 0

Query: 1   MNLDECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLS 60
           MNL E   MA +AG+E++EA+AHIWNH+FNFINSM+LKCA++LGIPDAIHRH PHPMPLS
Sbjct: 1   MNL-EGGKMAAIAGNELLEAQAHIWNHLFNFINSMTLKCAIQLGIPDAIHRHEPHPMPLS 60

Query: 61  QLVSAIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEK-EQQHEEEEGYVLTNSSRLLLK 120
           +LVSA+QLHPNKT FIYRLMR+LTHSGFFV QK+     +++ EEEEGYVLTNSSRLLLK
Sbjct: 61  RLVSALQLHPNKTQFIYRLMRLLTHSGFFVAQKVPKNSGKEEEEEEEGYVLTNSSRLLLK 120

Query: 121 HNPCA------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFN 180
           HNPC                    LS W +++D+TPF TAHG+P WE+LG K KDG+ FN
Sbjct: 121 HNPCGATPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPLWEFLGNKVKDGEGFN 180

Query: 181 AGMASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVV 240
           AGMASDARLVMSVLL+K+KGVFDG+ SLVDVGGGTGT+AK IA AFPQI+CTV DLPQVV
Sbjct: 181 AGMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVLDLPQVV 240

Query: 241 ADLKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTG 300
            +LKGE NLKYV+GDMF+AIPS  ALLLKWILHDWSD E V+ILK CKAAITS  +G  G
Sbjct: 241 GELKGEGNLKYVEGDMFKAIPSADALLLKWILHDWSDPECVRILKNCKAAITS--HGYKG 300

Query: 301 KVMVIDRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPI 353
           KVMVID VVG K  + DS ETQLF+DMLMMIL  G+EREE+EWA+LI+EAGFSGYNI PI
Sbjct: 301 KVMVIDMVVGYKGKESDSYETQLFYDMLMMILVGGKEREEKEWAKLIREAGFSGYNILPI 360

BLAST of Tan0015572 vs. ExPASy TrEMBL
Match: A0A6J1GKA3 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455047 PE=3 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 5.7e-147
Identity = 259/367 (70.57%), Postives = 298/367 (81.20%), Query Frame = 0

Query: 5   ECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLSQLVS 64
           E E M I+AGDEM+EA+AHIWNH+FNFINSM+LKCAVELGIPD IHRH PHPMPLS LVS
Sbjct: 4   EGEKMPILAGDEMLEAQAHIWNHLFNFINSMTLKCAVELGIPDTIHRHAPHPMPLSHLVS 63

Query: 65  AIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEK-EQQHEEEEGYVLTNSSRLLLKHNPC 124
           A+QLH NKT FIYRLMR+LTHSGFFV+QKL     +Q+ EEE+GYVLTNSSRLLLKHNPC
Sbjct: 64  ALQLHSNKTQFIYRLMRLLTHSGFFVVQKLPKNNGKQEEEEEDGYVLTNSSRLLLKHNPC 123

Query: 125 A------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFNAGMA 184
           A                   LS W +++D+TPF TAHG+PFWE+LG K KDG+ FN GMA
Sbjct: 124 AVTPFLLSMLQPALVEPWQLLSIWFQSEDRTPFHTAHGMPFWEFLGNKVKDGEGFNEGMA 183

Query: 185 SDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVADLK 244
           SDARLVMSVLL+K+KGVFDG+ SLVDVGGGTGT+AK IA AFPQI+CTVFDLPQVV +LK
Sbjct: 184 SDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVAKAIAEAFPQIECTVFDLPQVVGELK 243

Query: 245 GERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGKVMV 304
           GE NLKYV+GDMF+AIP    L+LKWILHDW+D+E  KILKKCK AI S  NG  GKV+V
Sbjct: 244 GEGNLKYVEGDMFKAIPPADVLVLKWILHDWNDDECAKILKKCKEAIRS--NGKKGKVIV 303

Query: 305 IDRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIMGLR 353
           ID VVGN K  D  I++QLFFDMLMM L +G+ER+E+EW++L KEAGF GY IFPI+G R
Sbjct: 304 IDMVVGNNKRDDTFIQSQLFFDMLMMGLVSGKERDEKEWSELFKEAGFGGYKIFPILGSR 363

BLAST of Tan0015572 vs. ExPASy TrEMBL
Match: A0A6J1GJY8 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455044 PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 3.1e-145
Identity = 255/370 (68.92%), Postives = 300/370 (81.08%), Query Frame = 0

Query: 1   MNLDECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLS 60
           MN +    + IVAGDE++EA+AHIWNH+ NFINSM+LKCAVELGIPDAIHRHGPHPMPLS
Sbjct: 5   MNFESENMLPIVAGDELLEAQAHIWNHLLNFINSMTLKCAVELGIPDAIHRHGPHPMPLS 64

Query: 61  QLVSAIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKH 120
           +LVSA+QLHP+KT FIYRLMR+LTHSGFFVLQKL     ++ EEEEGYVLTNSSRLLLK+
Sbjct: 65  RLVSALQLHPHKTRFIYRLMRLLTHSGFFVLQKLPKNSGEEEEEEEGYVLTNSSRLLLKY 124

Query: 121 NPCA------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFNA 180
           NPC                    LS W K++D+TPF+TAHG+ +WE++G K K GD+FNA
Sbjct: 125 NPCGVTPLLLSILQPALVEPWHLLSVWFKSEDRTPFDTAHGISYWEFMGSKPKHGDIFNA 184

Query: 181 GMASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVA 240
           GMASDARLVMSVLL+K+KGVFDG+ SLVDVGGGTGT+ K IA AFPQI+C VFDLPQVV 
Sbjct: 185 GMASDARLVMSVLLDKHKGVFDGVASLVDVGGGTGTVGKAIAEAFPQIECAVFDLPQVVG 244

Query: 241 DLKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGK 300
           +LKGE NLKYV+GDMF+AIP    L+LKWILHDW+DE+ VKILK CK AITS  NG  GK
Sbjct: 245 ELKGEGNLKYVEGDMFKAIPPADVLVLKWILHDWNDEDCVKILKNCKEAITS--NGKKGK 304

Query: 301 VMVIDRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIM 353
           V+VID VVG+K  +D  I++QL FDM+MM L  GRER+E+EW++  KEAGFSGY IFPI+
Sbjct: 305 VIVIDMVVGSKL-EDTFIQSQLCFDMVMMGLLNGRERDEKEWSEAFKEAGFSGYKIFPIL 364

BLAST of Tan0015572 vs. ExPASy TrEMBL
Match: A0A6J1I050 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111468226 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.5e-144
Identity = 255/370 (68.92%), Postives = 301/370 (81.35%), Query Frame = 0

Query: 1   MNLDECENMAIVAGDEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRHGPHPMPLS 60
           MN++  + + IVAG+E++EA+AHIWNH+ NFINSM+LKCAVELGIPDAIH H P PMPLS
Sbjct: 1   MNIEGEKMLPIVAGEELLEAQAHIWNHLLNFINSMTLKCAVELGIPDAIHHHAPRPMPLS 60

Query: 61  QLVSAIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKH 120
           +LVSA+QLHPNKTHFIYRLMR+LTHSGFFVLQKL    +   EEEEGYVLTNSSRLLLKH
Sbjct: 61  RLVSALQLHPNKTHFIYRLMRLLTHSGFFVLQKL---PKNSGEEEEGYVLTNSSRLLLKH 120

Query: 121 NPCA------------------FLSTWLKTDDQTPFETAHGLPFWEYLGKKSKDGDVFNA 180
           NPC                    LS W K+ D+TPF+ AHG+ FWE++G K KDGD+FN+
Sbjct: 121 NPCGVAPFLLSLLQPALVEPWHLLSVWFKSKDRTPFDMAHGMSFWEFMGSKPKDGDIFNS 180

Query: 181 GMASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVA 240
           GMASDARLVMS+LL+K+KGVFDG+ SLVD+GGGTGT+AK IA AFPQI+CTVFDLPQVV 
Sbjct: 181 GMASDARLVMSLLLDKHKGVFDGVASLVDIGGGTGTVAKAIAEAFPQIECTVFDLPQVVG 240

Query: 241 DLKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGK 300
           +LKGE NL+YV+GDMF+AIP    L+LKWILHDW+DE+ VKILK CKAAITS  NG  GK
Sbjct: 241 ELKGEGNLRYVEGDMFKAIPPADVLVLKWILHDWNDEDCVKILKNCKAAITS--NGKKGK 300

Query: 301 VMVIDRVVGNKKNKDDSIETQLFFDMLMMILQTGREREEEEWAQLIKEAGFSGYNIFPIM 353
           V+VID VVG+K  +D  I++QL FDM MM L  GRER+E+EW++  KEAGFSGYNIFPI+
Sbjct: 301 VIVIDMVVGSKL-EDTFIQSQLCFDMEMMSLVNGRERDEKEWSEAFKEAGFSGYNIFPIL 360

BLAST of Tan0015572 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 212.6 bits (540), Expect = 5.1e-55
Identity = 131/370 (35.41%), Postives = 195/370 (52.70%), Query Frame = 0

Query: 15  DEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRH-GPHPMPLSQLVSAIQLHPNKT 74
           DE  +A   IW ++F F +  + KCA++L IP+AI  H    P+ L++L SA+   P+  
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPS-- 83

Query: 75  HFIYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLL--------------LK 134
             + R+MR L H G F   K I  K+       GYV T  SR L              L 
Sbjct: 84  -HLRRIMRFLVHQGIF---KEIPTKDGL---ATGYVNTPLSRRLMITRRDGKSLAPFVLF 143

Query: 135 HNPCAFLSTWLKTDD----------QTPFETAHGLPFWEYLGKKSKDGDVFNAGMASDAR 194
                 L+ WL+               PF+  HG   W +        D+ N  MA DAR
Sbjct: 144 ETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDAR 203

Query: 195 LVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVADLKGERN 254
            V+  +     G+FDG+ ++VDVGGGTG     + + FP I+   FDLP V+   +    
Sbjct: 204 RVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDG 263

Query: 255 LKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGKVMVIDRV 314
           ++ V+GDMF++IP+  A+ +KW+LHDW D++ +KILK CK A+  N+    GKV++++ V
Sbjct: 264 VENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI----GKVLIVESV 323

Query: 315 VG-NKK-----NKDDSIE-TQLFFDMLMMI-LQTGREREEEEWAQLIKEAGFSGYNIFPI 352
           +G NKK      +D+ +E  +L  DM+MM    TG+ER  +EW  ++KEAGF+ Y +  I
Sbjct: 324 IGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDI 380

BLAST of Tan0015572 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 204.5 bits (519), Expect = 1.4e-52
Identity = 120/346 (34.68%), Postives = 184/346 (53.18%), Query Frame = 0

Query: 15  DEMVEAEAHIWNHIFNFINSMSLKCAVELGIPDAIHRH-GPHPMPLSQLVSAIQLHPNKT 74
           DE  +A   IW ++F F +  + KCA++L IP+AI  H    P+ LS+L SA+   P+  
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPS-- 69

Query: 75  HFIYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKHNPCAFLSTWLKTD 134
             + R+MR L H G F       E   +     GY  T  SR ++               
Sbjct: 70  -HLRRIMRFLVHQGLF------KEVPTKDGLATGYTNTPLSRRMM--------------- 129

Query: 135 DQTPFETAHGLPFWEYLGKKSKDGDVFNAGMASDARLVMSVLLEKYKGVFDGLNSLVDVG 194
                   HG   W +         + N  MA DAR V+  +    +G+FDG+ ++VDVG
Sbjct: 130 ----ITKLHGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQGLFDGVATVVDVG 189

Query: 195 GGTGTMAKTIARAFPQIQCTVFDLPQVVADLKGERNLKYVQGDMFEAIPSVHALLLKWIL 254
           GGTG     + + FP I+   FDLP V+   +    ++ V+GDMF++IP+  A+++KW+L
Sbjct: 190 GGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPASDAVIIKWVL 249

Query: 255 HDWSDEESVKILKKCKAAITSNVNGNTGKVMVIDRVVGNKKN------KDDSIE-TQLFF 314
           HDW D++ +KILK CK A+  N+    GKV++++ V+G KKN      +DD +E  +L  
Sbjct: 250 HDWGDKDCIKILKNCKEAVLPNI----GKVLIVECVIGEKKNTMIAEERDDKLEHVRLQL 309

Query: 315 DMLMMI-LQTGREREEEEWAQLIKEAGFSGYNIFPIMGLRSLIEIY 352
           DM+MM+   TG+ER  +EW  ++ EAGF+ Y +     ++SLI  Y
Sbjct: 310 DMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIAY 323

BLAST of Tan0015572 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 156.4 bits (394), Expect = 4.3e-38
Identity = 99/324 (30.56%), Postives = 165/324 (50.93%), Query Frame = 0

Query: 35  MSLKCAVELGIPDAIHRHGPHPMPLSQLVSAIQL-HPNKTHFIYRLMRILTHSGFFVL-- 94
           M+LK A+EL + + + ++G  PM  +++ S +   +P     + R++R+LT         
Sbjct: 34  MALKSALELDLLEIMAKNG-SPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSN 93

Query: 95  QKLISEKEQQ-----------HEEEEGYVLTNSSRLLLKHNPCAFLSTWLKTDDQ----- 154
           +KL  +  ++            + E+G    + + L L +     + +W    D      
Sbjct: 94  RKLSGDGVERIYGLGPVCKYLTKNEDG---VSIAALCLMNQDKVLMESWYHLKDAILDGG 153

Query: 155 TPFETAHGLPFWEYLGKKSKDGDVFNAGMASDARLVMSVLLEKYKGVFDGLNSLVDVGGG 214
            PF  A+G+  +EY G   +   VFN GM++ + + M  +LE YKG F+GL SLVDVGGG
Sbjct: 154 IPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGG 213

Query: 215 TGTMAKTIARAFPQIQCTVFDLPQVVADLKGERNLKYVQGDMFEAIPSVHALLLKWILHD 274
            G   K I   +P ++   FDLP V+ D      +++V GDMF ++P   A+ +KWI HD
Sbjct: 214 IGATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHD 273

Query: 275 WSDEESVKILKKCKAAITSNVNGNTGKVMVIDRVVGNKKNKDDSIETQLFFDMLMMILQT 334
           WSDE  VK LK C  ++  +     GKV++ + ++    +   S +  +  D +M+    
Sbjct: 274 WSDEHCVKFLKNCYESLPED-----GKVILAECILPETPDSSLSTKQVVHVDCIMLAHNP 333

Query: 335 -GREREEEEWAQLIKEAGFSGYNI 339
            G+ER E+E+  L K +GF G  +
Sbjct: 334 GGKERTEKEFEALAKASGFKGIKV 347

BLAST of Tan0015572 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 146.0 bits (367), Expect = 5.8e-35
Identity = 101/323 (31.27%), Postives = 166/323 (51.39%), Query Frame = 0

Query: 53  GPHPMPLSQLVSAIQLHPNKTHFIYRLMRILTHSGFFVLQKLISEKEQQH---------- 112
           G +  P+     A   +P+    I RL+R L         KL+ ++E +           
Sbjct: 51  GSYLSPVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTC-KLVKDEEGRESRAYGLGKVG 110

Query: 113 ----EEEEG-----YVLTNSSRLLLKHNPCAFLSTWLKTDDQTPFETAHGLPFWEYLGKK 172
               ++E+G     YVL   ++   K    ++L+  ++    + +E A+    +EY+ K 
Sbjct: 111 KKLIKDEDGFSIAPYVLAGCTK--AKGGVWSYLTEAIQEGGASAWERANEALIFEYMKKN 170

Query: 173 SKDGDVFNAGMASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCT 232
                +FN  M +   +VM  +LE Y G F+G++  VDVGG  G+    I   +P I+  
Sbjct: 171 ENLKKIFNESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGI 230

Query: 233 VFDLPQVVADLKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAIT 292
            FDLP +V +      ++++ GDMF+ IP    +L+KWILHDW+DE+ V+ILK CK A+ 
Sbjct: 231 NFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALP 290

Query: 293 SNVNGNTGKVMVIDRVVGNKKNKDD-SIETQLFFDMLMMILQT-GREREEEEWAQLIKEA 352
                 TG+++VI+ +V  + ++ D + +  L  D+ MM L + G+ER ++E+  L KEA
Sbjct: 291 -----ETGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEA 350

BLAST of Tan0015572 vs. TAIR 10
Match: AT5G53810.1 (O-methyltransferase family protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.3e-34
Identity = 113/370 (30.54%), Postives = 171/370 (46.22%), Query Frame = 0

Query: 12  VAGDEMVEAEAHIW-NHIFNFINS-MSLKCAVELGIPDAIHRHGPHPMPLSQLVSAIQLH 71
           V  ++ VE EA +    + N   S M LK A+ELG+ D I   G   + LS    A++L 
Sbjct: 15  VKEEQEVEEEARLLARRLANAAASPMVLKAALELGVIDTITTVGGGDLWLSPSEIALRL- 74

Query: 72  PNK------THFIYRLMRILTHSGFFVLQKLISEKEQQHEEEEGYVLTNSSRLLLKHN-- 131
           P K         + R++R L        + +I E  Q  + E  Y      + LL  +  
Sbjct: 75  PTKPCNLEAPALLDRMLRFLVSHSVLKCRTVIEENGQTGKVERVYAAEPVCKYLLNKSDD 134

Query: 132 -------------PCAFLSTWLKTDD-----QTPFETAHGLPFWEYLGKKSKDGDVFNAG 191
                           F+ TW   +D     +  F +AHG+  +EY+    + G VFN  
Sbjct: 135 VSGSFASLFMLDLSDVFIKTWTHLEDVILEGRDAFSSAHGMKLFEYIQADERFGKVFNRA 194

Query: 192 MASDARLVMSVLLEKYKGVFDGLNSLVDVGGGTGTMAKTIARAFPQIQCTVFDLPQVVAD 251
           M   + +V   +L+ Y+G F  + +LVDVGGG G     I   +P +    FDL  V+A+
Sbjct: 195 MLESSTMVTEKVLKFYEG-FKDVKTLVDVGGGLGNTLGLITSKYPHLIGINFDLAPVLAN 254

Query: 252 LKGERNLKYVQGDMFEAIPSVHALLLKWILHDWSDEESVKILKKCKAAITSNVNGNTGKV 311
                 + +V GDMF  IP   A+ +KWILHDW+DE+ V ILK C  ++  N     GK+
Sbjct: 255 AHSYPGVNHVAGDMFIKIPKGDAIFMKWILHDWTDEQCVAILKNCWKSLEEN-----GKL 314

Query: 312 MVIDRVVGNKKNKDDSIETQLF-FDMLMMI-LQTGREREEEEWAQLIKEAGFSGYNIFPI 352
           ++++ V   +    D     +F  DM M+     G+ER+  E+  L   +GFS   I   
Sbjct: 315 IIVEMVTPVEAKSGDICSNIVFGMDMTMLTQCSGGKERDLYEFENLAYASGFSRCAIVCA 374