Tan0015454 (gene) Snake gourd v1

Overview


Name	Tan0015454
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calcium-binding EF hand family protein
Location	LG07: 42965930 .. 42966788 (+)
RNA-Seq Expression	Tan0015454
Synteny	Tan0015454

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATCTCATATATAAACCCTTTCCAATCTCCAATTCACCACATTCAGTCCACAAAAACCAGTATCCATGTTCTTCCGATTGGAAGTTTTAATCACAATGCAGAACCACTTCCCCGGAGTCAACCTGCAGCTCGTCCCGACGCTCGTGCTCGTCGATCGAATATCGATATTGCCAAATCTGTTCTCCTTTGCAGACAGGATACATGACATGATTTCTCTACCTTGGTCTCTGTTTCATCAACCCCCCAAAGAAATTAAGGCTTTCCCACAAATCACTTCCTCACAATCTGAGGCCACTCAACACAAAAACAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAACTTGAATTATTTTGCAGCCCAGAAGAAGAGGAGGAAATGAAGGAATGTTTAGGAACTGATGAACTAATTACTGCCATTTTTTATGAGAGTGAGCCAAGCTTGGAAGAGTTGAATCAAACTTTTAATGTGTTTGATAAAAACAGAGATGGATTCATTGATGCAGAGGAGTTGTATACAGTGCTGGGCTTGCTTGGATCTAATAAAGGCATATTCCTCCATGACTGCCAAAGAATGATTGCCAGATTTGATCACAACAAAGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAATTGCCTTGTCCTGAGTATTATTTTGTCTAAACAAAGGTGTTTCAATTTTCATTTCCGTTGACATGTCCGACAGTTTTATTGACATTTGTATATGTCTATGGGTTGGACGTCGACATAAATGGTGAATTATTATTTTCGCCATATAATGAAAAGTTGACCAAAATATGAAATAAAATTTAAAAAATAACACTGTTGTGAACTAATTTT

mRNA sequence

CCATCTCATATATAAACCCTTTCCAATCTCCAATTCACCACATTCAGTCCACAAAAACCAGTATCCATGTTCTTCCGATTGGAAGTTTTAATCACAATGCAGAACCACTTCCCCGGAGTCAACCTGCAGCTCGTCCCGACGCTCGTGCTCGTCGATCGAATATCGATATTGCCAAATCTGTTCTCCTTTGCAGACAGGATACATGACATGATTTCTCTACCTTGGTCTCTGTTTCATCAACCCCCCAAAGAAATTAAGGCTTTCCCACAAATCACTTCCTCACAATCTGAGGCCACTCAACACAAAAACAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAACTTGAATTATTTTGCAGCCCAGAAGAAGAGGAGGAAATGAAGGAATGTTTAGGAACTGATGAACTAATTACTGCCATTTTTTATGAGAGTGAGCCAAGCTTGGAAGAGTTGAATCAAACTTTTAATGTGTTTGATAAAAACAGAGATGGATTCATTGATGCAGAGGAGTTGTATACAGTGCTGGGCTTGCTTGGATCTAATAAAGGCATATTCCTCCATGACTGCCAAAGAATGATTGCCAGATTTGATCACAACAAAGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAATTGCCTTGTCCTGAGTATTATTTTGTCTAAACAAAGGTGTTTCAATTTTCATTTCCGTTGACATGTCCGACAGTTTTATTGACATTTGTATATGTCTATGGGTTGGACGTCGACATAAATGGTGAATTATTATTTTCGCCATATAATGAAAAGTTGACCAAAATATGAAATAAAATTTAAAAAATAACACTGTTGTGAACTAATTTT

Coding sequence (CDS)

ATGTTCTTCCGATTGGAAGTTTTAATCACAATGCAGAACCACTTCCCCGGAGTCAACCTGCAGCTCGTCCCGACGCTCGTGCTCGTCGATCGAATATCGATATTGCCAAATCTGTTCTCCTTTGCAGACAGGATACATGACATGATTTCTCTACCTTGGTCTCTGTTTCATCAACCCCCCAAAGAAATTAAGGCTTTCCCACAAATCACTTCCTCACAATCTGAGGCCACTCAACACAAAAACAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAACTTGAATTATTTTGCAGCCCAGAAGAAGAGGAGGAAATGAAGGAATGTTTAGGAACTGATGAACTAATTACTGCCATTTTTTATGAGAGTGAGCCAAGCTTGGAAGAGTTGAATCAAACTTTTAATGTGTTTGATAAAAACAGAGATGGATTCATTGATGCAGAGGAGTTGTATACAGTGCTGGGCTTGCTTGGATCTAATAAAGGCATATTCCTCCATGACTGCCAAAGAATGATTGCCAGATTTGATCACAACAAAGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAATTGCCTTGTCCTGA

Protein sequence

MFFRLEVLITMQNHFPGVNLQLVPTLVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKEIKAFPQITSSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLEELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEFVKFMEIALS

Homology

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BLAST of Tan0015454 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.1e-22
Identity = 57/112 (50.89%), Postives = 76/112 (67.86%), Query Frame = 0


Query: 84  ISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLEELNQTFNVFDKNR 143
           IS+EE + VM  L LF + ++ +E        + ++++F E E SLEE+ Q F+VFD+N+
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQYSA----KEVSSLFEEKEASLEEVKQAFDVFDENK 142

Query: 144 DGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEFVKFME 196
           DGFIDA EL  VL +LG  +G +L +C  MI   D NKDGKIDFNEFVKFME
Sbjct: 143 DGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFME 190

[Close]

BLAST of Tan0015454 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 8.5e-20
Identity = 56/128 (43.75%), Postives = 80/128 (62.50%), Query Frame = 0


Query: 68  QITSSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEP 127
           Q +    E     +  + +E+V  VM+ L L  S ++E E  +   + + ++ +F E EP
Sbjct: 75  QTSIKHEEYRDDDDDGLCREDVGMVMKSLGL--STDQENEGLQKQYSSKEVSNLFEEKEP 134

Query: 128 SLEELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDF 187
           SLEE+ Q F+VFD+NRDGFID  +L  VL +LG  +G  L +C+RMI  FD +KDG+IDF
Sbjct: 135 SLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDF 194

Query: 188 NEFVKFME 196
             FVKFME
Sbjct: 195 YGFVKFME 200

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BLAST of Tan0015454 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.9e-11
Identity = 42/125 (33.60%), Postives = 70/125 (56.00%), Query Frame = 0


Query: 81  NSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTD-------ELITAIF---YESEPSLE 140
           +  I+K+E+   +E L ++   ++  +M E +  +       E    ++    E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 141 ELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEF 196
           ++ + FNVFD+NRDGFI  EEL +VL  LG  +G  L DC+RMI++ D + DG ++F EF
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEF 137

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BLAST of Tan0015454 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.9e-11
Identity = 46/147 (31.29%), Postives = 75/147 (51.02%), Query Frame = 0


Query: 60  PKEIKAFPQITSSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKE--------C 119
           P E+K   Q+     + T      I+ +E+   +  L ++   +E  +M E        C
Sbjct: 3   PTELKRVFQMFDKNGDGT------ITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGC 62

Query: 120 LGTD---ELITAIFYESEPSLEELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLH 179
           +  D   EL   I  E +   E++ + FNVFD+N DGFI  +EL  VL  LG  +G  L 
Sbjct: 63  VDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLD 122

Query: 180 DCQRMIARFDHNKDGKIDFNEFVKFME 196
           DC++MI + D + DG++++ EF + M+
Sbjct: 123 DCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of Tan0015454 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 7.2e-11
Identity = 46/117 (39.32%), Postives = 68/117 (58.12%), Query Frame = 0


Query: 85  SKEEVKFVMEKLELF--CSPEEEEEMKECLGTDELITAIFYESEPSLEELNQTFNVFDKN 144
           S EEVK +++  E    C  EE EE    L  +E++           E + + F +FD+N
Sbjct: 79  SVEEVKAIIDDSEALYECLIEEGEEY--LLEKNEMM---------GKEIVKEAFRLFDEN 138

Query: 145 RDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEFVKFMEIALS 200
           +DGFID  EL  VL LLG ++   + +C++M+  +D N+DGKIDF EFVK +E + S
Sbjct: 139 QDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]

BLAST of Tan0015454 vs. NCBI nr
Match: XP_023003075.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 271.6 bits (693), Expect = 5.6e-69
Identity = 146/189 (77.25%), Postives = 157/189 (83.07%), Query Frame = 0


Query: 11  MQNHFPGVNLQLVPTLVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKEIKAFPQIT 70
           M + FP VNL+L PTL+LVDRISI   LFSF DRIHD+IS PWSLFH PPKE K  PQ  
Sbjct: 1   MPSQFPIVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKE-KVSPQGN 60

Query: 71  SSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLE 130
           S +SEATQHKNSSISKEEVKFVMEKLE FCS EE EE    LG +E I AIFYESEPSLE
Sbjct: 61  SLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEE--SWLGGEE-IGAIFYESEPSLE 120

Query: 131 ELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEF 190
           EL QTFNVFDKNRDG IDA+EL +VLGLL  N+G+F+HDC RMIARFDHNKDGKIDFNEF
Sbjct: 121 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 180

Query: 191 VKFMEIALS 200
           VKFMEIALS
Sbjct: 181 VKFMEIALS 185

[Close]

BLAST of Tan0015454 vs. NCBI nr
Match: XP_023530007.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.0 bits (689), Expect = 1.6e-68
Identity = 144/189 (76.19%), Postives = 158/189 (83.60%), Query Frame = 0


Query: 11  MQNHFPGVNLQLVPTLVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKEIKAFPQIT 70
           M + FP VNL+L PTL+LVDRISI   LFSF DRIHD+IS PWSLFH PPKE KA PQ  
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKE-KASPQGN 60

Query: 71  SSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLE 130
           S +SEATQHKNSSISKEEVKFVMEKLE FCS EE +E    LG +E I AIFYESEPSLE
Sbjct: 61  SLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDE--SWLGGEE-IGAIFYESEPSLE 120

Query: 131 ELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEF 190
           EL QTFNVFDKNRDG IDA+EL ++LGLL  N+G+F+HDC RMIAR+DHNKDGKIDFNEF
Sbjct: 121 ELKQTFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 180

Query: 191 VKFMEIALS 200
           VKFMEIALS
Sbjct: 181 VKFMEIALS 185

[Close]

BLAST of Tan0015454 vs. NCBI nr
Match: KAG6588366.1 (putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 269.6 bits (688), Expect = 2.1e-68
Identity = 146/189 (77.25%), Postives = 157/189 (83.07%), Query Frame = 0


Query: 11  MQNHFPGVNLQLVPTLVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKEIKAFPQIT 70
           M + FP VNL+L PTL+LVDRISI   LFSF DRIHD+IS PWSLFH PPKE KA PQ  
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKE-KASPQGN 60

Query: 71  SSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLE 130
           S +SEATQHKNSSISKEEVKFVMEKLE FCS EE EE    LG +E I AIF ESEPSLE
Sbjct: 61  SLESEATQHKNSSISKEEVKFVMEKLEFFCSEEEIEE--SWLGGEE-IGAIFDESEPSLE 120

Query: 131 ELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEF 190
           EL QTFNVFDKNRDG IDA+EL +VLGLL  N+G+F+HDC RMIARFDHNKDGKIDFNEF
Sbjct: 121 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 180

Query: 191 VKFMEIALS 200
           VKFMEIALS
Sbjct: 181 VKFMEIALS 185

[Close]

BLAST of Tan0015454 vs. NCBI nr
Match: XP_022927644.1 (probable calcium-binding protein CML30 [Cucurbita moschata])

HSP 1 Score: 269.6 bits (688), Expect = 2.1e-68
Identity = 146/189 (77.25%), Postives = 157/189 (83.07%), Query Frame = 0


Query: 11  MQNHFPGVNLQLVPTLVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKEIKAFPQIT 70
           M + FP VNL+L  TL+LVDRISI   LFSF DRIHD+IS PWSLFH PPKE KA PQ  
Sbjct: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKE-KASPQGN 60

Query: 71  SSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLE 130
           S +SEATQHKNSSISKEEVKFVMEKLE FCS EE EE    LG +E I AIFYESEPSLE
Sbjct: 61  SLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEE--SWLGGEE-IGAIFYESEPSLE 120

Query: 131 ELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEF 190
           EL QTFNVFDKNRDG IDA+EL +VLGLL  N+G+F+HDC RMIARFDHNKDGKIDFNEF
Sbjct: 121 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 180

Query: 191 VKFMEIALS 200
           VKFMEIALS
Sbjct: 181 VKFMEIALS 185

[Close]

BLAST of Tan0015454 vs. NCBI nr
Match: KAG7022215.1 (putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 267.7 bits (683), Expect = 8.0e-68
Identity = 145/189 (76.72%), Postives = 157/189 (83.07%), Query Frame = 0


Query: 11  MQNHFPGVNLQLVPTLVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKEIKAFPQIT 70
           M + FP VNL+L PTL+LVDRISI   LFSF DRIHD+IS PWSLFH PPKE KA PQ  
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKE-KASPQGN 60

Query: 71  SSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLE 130
           S +SEATQHK+SSISKEEVKFVMEKLE FCS EE EE    LG +E I AIF ESEPSLE
Sbjct: 61  SLESEATQHKSSSISKEEVKFVMEKLEFFCSEEEIEE--SWLGGEE-IGAIFDESEPSLE 120

Query: 131 ELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEF 190
           EL QTFNVFDKNRDG IDA+EL +VLGLL  N+G+F+HDC RMIARFDHNKDGKIDFNEF
Sbjct: 121 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 180

Query: 191 VKFMEIALS 200
           VKFMEIALS
Sbjct: 181 VKFMEIALS 185

[Close]

BLAST of Tan0015454 vs. ExPASy TrEMBL
Match: A0A6J1KQS0 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111496787 PE=4 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 2.7e-69
Identity = 146/189 (77.25%), Postives = 157/189 (83.07%), Query Frame = 0


Query: 11  MQNHFPGVNLQLVPTLVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKEIKAFPQIT 70
           M + FP VNL+L PTL+LVDRISI   LFSF DRIHD+IS PWSLFH PPKE K  PQ  
Sbjct: 1   MPSQFPIVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKE-KVSPQGN 60

Query: 71  SSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLE 130
           S +SEATQHKNSSISKEEVKFVMEKLE FCS EE EE    LG +E I AIFYESEPSLE
Sbjct: 61  SLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEE--SWLGGEE-IGAIFYESEPSLE 120

Query: 131 ELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEF 190
           EL QTFNVFDKNRDG IDA+EL +VLGLL  N+G+F+HDC RMIARFDHNKDGKIDFNEF
Sbjct: 121 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 180

Query: 191 VKFMEIALS 200
           VKFMEIALS
Sbjct: 181 VKFMEIALS 185

[Close]

BLAST of Tan0015454 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.0e-68
Identity = 146/189 (77.25%), Postives = 157/189 (83.07%), Query Frame = 0


Query: 11  MQNHFPGVNLQLVPTLVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKEIKAFPQIT 70
           M + FP VNL+L  TL+LVDRISI   LFSF DRIHD+IS PWSLFH PPKE KA PQ  
Sbjct: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKE-KASPQGN 60

Query: 71  SSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLE 130
           S +SEATQHKNSSISKEEVKFVMEKLE FCS EE EE    LG +E I AIFYESEPSLE
Sbjct: 61  SLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEE--SWLGGEE-IGAIFYESEPSLE 120

Query: 131 ELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEF 190
           EL QTFNVFDKNRDG IDA+EL +VLGLL  N+G+F+HDC RMIARFDHNKDGKIDFNEF
Sbjct: 121 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 180

Query: 191 VKFMEIALS 200
           VKFMEIALS
Sbjct: 181 VKFMEIALS 185

[Close]

BLAST of Tan0015454 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 6.7e-52
Identity = 117/196 (59.69%), Postives = 145/196 (73.98%), Query Frame = 0


Query: 11  MQNHFPGVNLQLVPT-------LVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKEI 70
           M N  P +NLQL+P+       LV +DR SI      FA  IHD++SL +S F  PPK+ 
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASI------FAGTIHDLVSLAFSFFQSPPKD- 60

Query: 71  KAFPQITSSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFY 130
           KA PQ  +SQSE+ +  +  +S+EEVK VMEKLEL+C+ EE++E+ EC G DE +  +F 
Sbjct: 61  KAAPQSRASQSESKE--DWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDE-VRGMFE 120

Query: 131 ESEPSLEELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDG 190
           E+EPSLEEL QTFNVFD+NRDGFIDA+EL+TVLGLL SN  IF+HDC+ MIARFD N D 
Sbjct: 121 ENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQ 180

Query: 191 KIDFNEFVKFMEIALS 200
           KIDFNEFVKFME+ALS
Sbjct: 181 KIDFNEFVKFMEVALS 186

[Close]

BLAST of Tan0015454 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 1.3e-50
Identity = 116/197 (58.88%), Postives = 143/197 (72.59%), Query Frame = 0


Query: 11  MQNHFPGVNLQLVPT--------LVLVDRISILPNLFSFADRIHDMISLPWSLFHQPPKE 70
           M N  P +NLQL+P+        LV +DR SI      FAD IHD++SL +S F  PPKE
Sbjct: 1   MHNPQPTINLQLIPSTTTMMPPPLVFIDRASI------FADTIHDLVSLAFSFFQSPPKE 60

Query: 71  IKAFPQITSSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIF 130
            KA PQ  +SQSE  +  +  +S+EEVK VMEKLEL+C+ E ++E+ EC G +E +  +F
Sbjct: 61  -KAAPQSRASQSEGKE--DWLMSREEVKSVMEKLELYCNEEGDDEIDECFGREE-VRGMF 120

Query: 131 YESEPSLEELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKD 190
            E+EPSLEEL QTFNVFD+NRDGFIDA EL+TVLGLL SN  IF+HDC+ MIARFD N D
Sbjct: 121 EENEPSLEELKQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNND 180

Query: 191 GKIDFNEFVKFMEIALS 200
            KIDFNEFVKFME++LS
Sbjct: 181 EKIDFNEFVKFMEVSLS 187

[Close]

BLAST of Tan0015454 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 5.8e-48
Identity = 122/211 (57.82%), Postives = 147/211 (69.67%), Query Frame = 0


Query: 1   MFFRLE-VLITMQNHFPGV-NLQLVPT-LVLVDRISILPNLFSFADRIHDMI-SLPWSLF 60
           MFFRLE  LITM+     + ++QL P+  V VDRISILP LF+FADRI +++ SLPW + 
Sbjct: 1   MFFRLEYYLITMETRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPIL 60

Query: 61  HQPPKEIKAFPQITSSQSE------ATQHKNSSISKEEVKFVMEKLELFCSPEEE--EEM 120
            QP  + KA  Q   S          TQ   SS+S+EEV+FVMEKLELF   E +     
Sbjct: 61  -QPHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGD 120

Query: 121 KECLGTDELITAIFYESEPSLEELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLH 180
           K    +DE+I  +F E+EPSLEEL QTFNVFD+NRDGFID  EL+ VL LL SNKGIF+H
Sbjct: 121 KGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIH 180

Query: 181 DCQRMIARFDHNKDGKIDFNEFVKFMEIALS 200
           DC+ MIARFD N DGKIDF+EFVKFME+ALS
Sbjct: 181 DCKTMIARFDLNNDGKIDFHEFVKFMEVALS 210

[Close]

BLAST of Tan0015454 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 106.3 bits (264), Expect = 2.9e-23
Identity = 57/112 (50.89%), Postives = 76/112 (67.86%), Query Frame = 0


Query: 84  ISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEPSLEELNQTFNVFDKNR 143
           IS+EE + VM  L LF + ++ +E        + ++++F E E SLEE+ Q F+VFD+N+
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQYSA----KEVSSLFEEKEASLEEVKQAFDVFDENK 142

Query: 144 DGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEFVKFME 196
           DGFIDA EL  VL +LG  +G +L +C  MI   D NKDGKIDFNEFVKFME
Sbjct: 143 DGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFME 190

[Close]

BLAST of Tan0015454 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 98.6 bits (244), Expect = 6.0e-21
Identity = 56/128 (43.75%), Postives = 80/128 (62.50%), Query Frame = 0


Query: 68  QITSSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTDELITAIFYESEP 127
           Q +    E     +  + +E+V  VM+ L L  S ++E E  +   + + ++ +F E EP
Sbjct: 75  QTSIKHEEYRDDDDDGLCREDVGMVMKSLGL--STDQENEGLQKQYSSKEVSNLFEEKEP 134

Query: 128 SLEELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDF 187
           SLEE+ Q F+VFD+NRDGFID  +L  VL +LG  +G  L +C+RMI  FD +KDG+IDF
Sbjct: 135 SLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDF 194

Query: 188 NEFVKFME 196
             FVKFME
Sbjct: 195 YGFVKFME 200

[Close]

BLAST of Tan0015454 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 70.9 bits (172), Expect = 1.3e-12
Identity = 46/147 (31.29%), Postives = 75/147 (51.02%), Query Frame = 0


Query: 60  PKEIKAFPQITSSQSEATQHKNSSISKEEVKFVMEKLELFCSPEEEEEMKE--------C 119
           P E+K   Q+     + T      I+ +E+   +  L ++   +E  +M E        C
Sbjct: 3   PTELKRVFQMFDKNGDGT------ITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGC 62

Query: 120 LGTD---ELITAIFYESEPSLEELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLH 179
           +  D   EL   I  E +   E++ + FNVFD+N DGFI  +EL  VL  LG  +G  L 
Sbjct: 63  VDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLD 122

Query: 180 DCQRMIARFDHNKDGKIDFNEFVKFME 196
           DC++MI + D + DG++++ EF + M+
Sbjct: 123 DCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of Tan0015454 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 70.9 bits (172), Expect = 1.3e-12
Identity = 42/125 (33.60%), Postives = 70/125 (56.00%), Query Frame = 0


Query: 81  NSSISKEEVKFVMEKLELFCSPEEEEEMKECLGTD-------ELITAIF---YESEPSLE 140
           +  I+K+E+   +E L ++   ++  +M E +  +       E    ++    E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 141 ELNQTFNVFDKNRDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEF 196
           ++ + FNVFD+NRDGFI  EEL +VL  LG  +G  L DC+RMI++ D + DG ++F EF
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEF 137

[Close]

BLAST of Tan0015454 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 68.9 bits (167), Expect = 5.1e-12
Identity = 46/117 (39.32%), Postives = 68/117 (58.12%), Query Frame = 0


Query: 85  SKEEVKFVMEKLELF--CSPEEEEEMKECLGTDELITAIFYESEPSLEELNQTFNVFDKN 144
           S EEVK +++  E    C  EE EE    L  +E++           E + + F +FD+N
Sbjct: 79  SVEEVKAIIDDSEALYECLIEEGEEY--LLEKNEMM---------GKEIVKEAFRLFDEN 138

Query: 145 RDGFIDAEELYTVLGLLGSNKGIFLHDCQRMIARFDHNKDGKIDFNEFVKFMEIALS 200
           +DGFID  EL  VL LLG ++   + +C++M+  +D N+DGKIDF EFVK +E + S
Sbjct: 139 QDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

The following BLAST results are available for this feature:

BLAST of Tan0015454 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	4.1e-22	50.89	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	8.5e-20	43.75	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9SRR7	1.9e-11	33.60	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9LNE7	1.9e-11	31.29	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SN89	7.2e-11	39.32	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]

BLAST of Tan0015454 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023003075.1	5.6e-69	77.25	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]
XP_023530007.1	1.6e-68	76.19	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]
KAG6588366.1	2.1e-68	77.25	putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022927644.1	2.1e-68	77.25	probable calcium-binding protein CML30 [Cucurbita moschata]	[more]
KAG7022215.1	8.0e-68	76.72	putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. a...	[more]

BLAST of Tan0015454 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KQS0	2.7e-69	77.25	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114967...	[more]
A0A6J1EIK8	1.0e-68	77.25	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1F854	6.7e-52	59.69	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1J5Q6	1.3e-50	58.88	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A0A0LHQ5	5.8e-48	57.82	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]

BLAST of Tan0015454 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	2.9e-23	50.89	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	6.0e-21	43.75	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	1.3e-12	31.29	EF hand calcium-binding protein family	[more]
AT3G07490.1	1.3e-12	33.60	ARF-GAP domain 11	[more]
AT3G47480.1	5.1e-12	39.32	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 169..197 e-value: 8.9E-4 score: 28.6 coord: 131..159 e-value: 1.2E-5 score: 34.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 130..195 e-value: 6.7E-11 score: 42.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..162 score: 15.127104
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 165..199 score: 11.361225
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 131..194 e-value: 7.78355E-16 score: 66.8025
None	No IPR available	GENE3D	1.10.238.10		coord: 60..196 e-value: 1.7E-21 score: 78.6
None	No IPR available	PANTHER	PTHR10891:SF805	CALCIUM-BINDING PROTEIN CML45-RELATED	coord: 73..197
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 73..197
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 178..190
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 79..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0015454.1	Tan0015454.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0005509	calcium ion binding