Tan0015445 (gene) Snake gourd v1

BLAST of Tan0015445 vs. ExPASy Swiss-Prot
Match: F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 2.1e-182
Identity = 331/527 (62.81%), Postives = 398/527 (75.52%), Query Frame = 0

Query: 14  AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGS 73
           +YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + +M VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSE 133
           TCTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIASLGYDHME 193
           ++PMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN VQ P VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 LLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSLDPKLLHG 253
           +LG TLG+IA EKAGIFK G PAFTV QPDEAMRVL +KAS  +V L VV  L  +LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 RKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEGLTTASFEGRAQ 313
           +KLGL+GEHQY+NA LA++L+S WL + G  ++    Q   LPE+FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIK-RNQQGLNPQLEDNGTSSNK 373
           +VPD+Y +  +  DLVF+LDGAHSPESME C  WFS A+K  NQ G +  L +    S+ 
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 374 LLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKALFVPNTSVFNK 433
               + N+       Q+LLFNCM+VRDP LLLP L   CA  GV+F KALFVPN SV++K
Sbjct: 426 --DKWSNETCE----QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHK 485

Query: 434 VGTHG-LPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESEKPEVGGKSCE 493
           VGT   LPE + QVDLSWQF +++VWE+++Q ++                  E  G+S  
Sbjct: 486 VGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESDG 545

Query: 494 NSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           NS VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 NSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

BLAST of Tan0015445 vs. ExPASy Swiss-Prot
Match: Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 3.2e-162
Identity = 301/525 (57.33%), Postives = 376/525 (71.62%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LE+++SQMK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSED 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 VPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIASLGYDHMEL 194
           VPMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV+Q P VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSLDPKLLHGR 254
           LGYTL EIA+EKAGIFK G PAFTV QPDEAMRVL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNA LA+AL ST+L   G    + ++Q+  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQGLNPQLEDNGTSSNKLL 374
           VPD     E PE++V++LDGAHSPESME C  WFS  IK+NQ                  
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ------------------ 372

Query: 375 QGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKALFVPNTSVFNKVG 434
                +  +K+S Q+LLFNCM+VRDP LLLP L   C + GV F +A+FVPN SV+N+VG
Sbjct: 373 -----ERNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVG 432

Query: 435 THGLPEANGQVD-LSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESEKPEVGGKSCENS 494
           +        +V+ +SWQF ++R+WE++ + +  S+SK             +  GK  E S
Sbjct: 433 SS--TNVGTRVESMSWQFGLQRIWESLARGEAKSNSKS------------DSKGKEEEKS 491

Query: 495 TVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

BLAST of Tan0015445 vs. ExPASy Swiss-Prot
Match: F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.2e-150
Identity = 288/552 (52.17%), Postives = 380/552 (68.84%), Query Frame = 0

Query: 13  TAYEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEESMSQMKVIHVAGTK 72
           ++Y++A++ALS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 73  GKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKE 132
           GKGSTC F+E+ILRNCGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 133 KTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIASLGY 192
           K  + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV+Q P VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 193 DHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSLDPK 252
           DHM++LG TL +IA  KAGIFK   PAFTV Q  EAM VL+  A+ L+V L VV  L+PK
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320

Query: 253 LLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQS--GSLPEQFIEGLTTAS 312
            L G  LGL G+HQ +NA LA++LS  WL RTG+ K  +  +S    +P  F  GL TA 
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380

Query: 313 FEGRAQIVPDRYID-------IESP-EDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQGL 372
             GRAQ+V D   D       +E+P  DL+F+LDGAHSPESME CG WFS+A+ R  + L
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAV-RGDKSL 440

Query: 373 NPQLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFN 432
           +  +      +  +  G    +  + S Q+LLFNCM VRDPQ+LLP L+ TCA++G HF+
Sbjct: 441 STAV------NGYMRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTHFS 500

Query: 433 KALFVPNTSVFNKV--GTHGLPEANGQVDLSWQFHVRRVWENIMQ-CDKGSD----SKGI 492
           +ALFVP+ S +NKV  G   +P    + DL+WQF ++R+WE  +Q  D G D      GI
Sbjct: 501 RALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKPDGI 560

Query: 493 GAMLDEE---SEKPEVGGKS---CENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGS 537
            A+   +    + P+ GG +     +S V  SLP+ I WLRD V ++N S++L+VLVTGS
Sbjct: 561 TALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLVTGS 620

BLAST of Tan0015445 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.2e-81
Identity = 212/582 (36.43%), Postives = 297/582 (51.03%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRRCD----KSGD---RFELMFEYVKMLDLE-ESMSQMKVIHVAGT 74
           Y++A+  L++L  N    +    K GD   + E M  Y+    L+ E + Q+ +IH+ GT
Sbjct: 42  YQDAVRTLNTLQTNANYLEMVKRKRGDPQTQLEAMKLYLARSGLQVEDLDQLNIIHITGT 101

Query: 75  KGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLK 134
           KGKGSTC FTE ILR+ G  TG F+SPHL+ +RER R++G  IS E F  +FW  Y+RL+
Sbjct: 102 KGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRINGQPISSELFTKHFWRLYHRLE 161

Query: 135 EKTSED--VPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIAS 194
           E   +   V MP  FRFL L+AF +F +E+VD+A+LEVG+GG +D TN+++ P VCGI S
Sbjct: 162 ETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGIGGAYDCTNIIRKPVVCGITS 221

Query: 195 LGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSL 254
           LG DH  LLG T+ +IA +K GIFK G PAFTV QPDE + VLRD+A ++   L + P L
Sbjct: 222 LGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPLAVLRDRAQQISCPLYLCPPL 281

Query: 255 DPKLLHG--RKLGLEGEHQYLNASLAIALSSTWLHRTGHS-----KMSYVEQSGSLP--- 314
                 G    LGLEGEHQ  NA+LA+ ++  WL + G+      K S     G LP   
Sbjct: 282 QALEEGGPPLTLGLEGEHQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAP 341

Query: 315 -----EQFIEGLTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAA 374
                 +  +GL    + GR Q++            L ++LDGAH+  SM+ C  WF  A
Sbjct: 342 VFQPTPRMQQGLRHTEWPGRTQLL--------RRGPLTWYLDGAHTTSSMQACVRWFRQA 401

Query: 375 IKRNQQGLNPQLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTC 434
           + R ++       D+G+                  V+VLLFN    RD   LL  L++ C
Sbjct: 402 LHRCER------PDSGS-----------------EVRVLLFNSTGDRDSAALL-KLLQPC 461

Query: 435 ANNGVHFNKALFVPNTSVFNKVGTHGLPEANGQVD--------LSWQFHVRRVWENIMQC 494
                 F+ A+F PN +      T    + N  V         L+ Q H  R+ E  +  
Sbjct: 462 -----QFDYAIFCPNLA--EMASTDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSP 521

Query: 495 D----KGSDSKGIGAMLDEESEKPEVGGKSCENSTVFSSLPMAIKWL---RDSVLQQ--- 537
           D     G +  G  ++L      P        +S VFS +  A++W+   RD + Q    
Sbjct: 522 DPWSTPGQEQDGPASLL----LAPHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSP 580

BLAST of Tan0015445 vs. ExPASy Swiss-Prot
Match: P48760 (Folylpolyglutamate synthase, mitochondrial OS=Mus musculus OX=10090 GN=Fpgs PE=1 SV=3)

HSP 1 Score: 299.7 bits (766), Expect = 6.7e-80
Identity = 205/581 (35.28%), Postives = 293/581 (50.43%), Query Frame = 0

Query: 15  YEEALDALSSLIVN--------RRRCDKSGDRFELMFEYVKMLDLE-ESMSQMKVIHVAG 74
           Y++A+  L++L  N        R+R D    + E M  Y+    L+ E ++++ +IHV G
Sbjct: 45  YQDAVRTLNTLQTNASYLEQVKRQRSDPQA-QLEAMEMYLARSGLQVEDLNRLNIIHVTG 104

Query: 75  TKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRL 134
           TKGKGSTC FTE ILRN G  TG F+SPH++ +RER R++G  IS E F  +FW  YN+L
Sbjct: 105 TKGKGSTCAFTERILRNYGLKTGFFSSPHMVQVRERIRINGKPISPELFTKHFWCLYNQL 164

Query: 135 KE-KTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIAS 194
           +E K    V MP  FRFL L+AF +F +E+VD+A++EVG+GG FD TN+++ P VCG++S
Sbjct: 165 EEFKDDSHVSMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVVCGVSS 224

Query: 195 LGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSL 254
           LG DH  LLG T+ +IA +K GIFK G PAFTV QP+  + VLRD+A ++   L + P L
Sbjct: 225 LGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVVQPEGPLAVLRDRAQQIGCPLYLCPPL 284

Query: 255 DPKLLHG--RKLGLEGEHQYLNASLAIALSSTWLHRTGHS-----KMSYVEQSGSLP--- 314
           +     G    LGLEG HQ  NA+LA+ L+  WL R  H      K+S       LP   
Sbjct: 285 EALEEVGLPLSLGLEGAHQRSNAALALQLAHCWLERQDHQDIQELKVSRPSIRWQLPLAP 344

Query: 315 -----EQFIEGLTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAA 374
                     GL    + GR QI+            L ++LDGAH+  S++ C  W+  +
Sbjct: 345 VFRPTPHMRRGLRDTVWPGRTQIL--------QRGPLTWYLDGAHTTSSVQACVHWYRQS 404

Query: 375 IKRNQQGLNPQLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTC 434
           ++R+++       D G+                  V +LLFN    RD   LL  L++ C
Sbjct: 405 LERSKR------TDGGS-----------------EVHILLFNSTGDRDSAALL-KLLQPC 464

Query: 435 ANNGVHFNKALFVPNTSVFNKVGTHGLPEANGQVDLS------------WQFHVRRVWEN 494
                 F+ A+F PN +  + +G     + N  V L             W     +   +
Sbjct: 465 -----QFDYAVFCPNVTEVSSIG--NADQQNFTVTLDQVLLRCLQHQQHWNGLAEKQASS 524

Query: 495 IMQCDKGSDSKGIGAMLDEESEKPEVGGKSCENSTVFSSLPMAIKWL---RDSVLQQ--- 536
            +    G D  G G++L      P     +  +S VFS +  A+ W+   RD + Q    
Sbjct: 525 NLWSSCGPDPAGPGSLL----LAPHPPQPTRTSSLVFSCISHALLWISQGRDPIFQPQSL 581

BLAST of Tan0015445 vs. NCBI nr
Match: XP_022990580.1 (folylpolyglutamate synthase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 968.4 bits (2502), Expect = 2.6e-278
Identity = 484/537 (90.13%), Postives = 501/537 (93.30%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           M EG   LK SRTAYEEALD L SLIVNRRRCDKSGDRFELMFEYVKMLDLEESMS+MKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKEKTSEDVPMP LFRFLALLAFKIFS+EQVDVAILEVGLGGTFDATNVVQAP VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDH ELLG TLG IASEKAGIFKRGAPAFTVFQPDEAM VLRDKAS+LDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           VPSLDP+LL G KLGLEGEHQYLNA LAIALSSTWLHRTG S +SY EQSGSLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQG-LNP 360
           LTTA FEGRAQIVPDRY DIE+PEDLVF+LDGAHSPESM VC SWF AAIK NQ+G LN 
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 361 QLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKA 420
           QL+DNGTSSNKLLQGYDNDEARKKSVQVLLFNCM+VRDPQLLL PLIKTCANNGVHF KA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESE 480
           LFVPNTS+FNKVGTHGL EANG+VDLSWQFHVRRVWENI+Q +KG DS+GIGAM++EESE
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           KPEV GKSCENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Tan0015445 vs. NCBI nr
Match: XP_023533507.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 966.5 bits (2497), Expect = 9.8e-278
Identity = 483/537 (89.94%), Postives = 501/537 (93.30%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           M EG   LK SRTAYEEALD LSSLIV+RRRCDKSGDRFELMFEYVKMLDLEESMS+MKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKEKTSEDVPMP LFRFLALLAFKIFS+EQVDVAILEVGLGGTFDATNVVQAP VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDH ELLG TLG IASEKAGIFKRGAPAFTV QPDEAM VLRDKAS+LDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           VPSLDP+LL G KLGLEGEHQYLNA LAIALSSTWLHRTGHS +SY EQSGSLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQG-LNP 360
           LTTASFEGRAQIVPDRY DIESPEDLVF+LDGAHSPESM VC SWF AAIK NQ+G L+ 
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKA 420
           QL+DNGTSSNKLLQGYDNDEARKKSVQVLLFNCM+VRDPQLLL PLIKTCANNGVHF KA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESE 480
           +FVPNTSVFNKVGTHGL EANG+VDLSWQFHVRRVWENI+Q +KG DS+GIG M++EESE
Sbjct: 421 VFVPNTSVFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGTMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           KPEV GK CENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Tan0015445 vs. NCBI nr
Match: XP_038889970.1 (folylpolyglutamate synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 964.9 bits (2493), Expect = 2.8e-277
Identity = 482/538 (89.59%), Postives = 502/538 (93.31%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEG +TLK SRTAYEEALDALS LIVNRRRCDKSGDRFELMFEYVKMLDLEESM QMKV
Sbjct: 1   MVEGNETLKDSRTAYEEALDALSLLIVNRRRCDKSGDRFELMFEYVKMLDLEESMLQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRN GFHTGLFTSPHLIDIRERFRLDG+DISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNYGFHTGLFTSPHLIDIRERFRLDGVDISEEKFLTYFWW 120

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKEKTSEDVPMP LFRFLALLAFKIFS+EQVDVAILEVGLGGTFDATNVVQAP VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPVVC 180

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDHMELLG TLGEIASEKAGIFK GAPAFTVFQPDEAMRVLRDKAS+LDVQLT 
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKHGAPAFTVFQPDEAMRVLRDKASQLDVQLTT 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           V  LDP+LLHGRKLGLEGEHQYLNA LAIALSSTWLHR GHS++SYVEQ+ SLPEQFIEG
Sbjct: 241 VSPLDPELLHGRKLGLEGEHQYLNAGLAIALSSTWLHRAGHSELSYVEQNRSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQGLNPQ 360
           LTTASFEGRAQIVPD+YID+ESP+DLVF+LDGAHSPESME CG WFSAAI+ NQ G   Q
Sbjct: 301 LTTASFEGRAQIVPDKYIDVESPDDLVFYLDGAHSPESMEACGRWFSAAIRHNQPGNLNQ 360

Query: 361 LEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKAL 420
            EDN TSSNK LQGYDNDEARKKSVQVLLFNCM+VRDPQLLLPPLIK CANNGV+F KAL
Sbjct: 361 SEDNATSSNK-LQGYDNDEARKKSVQVLLFNCMSVRDPQLLLPPLIKMCANNGVNFKKAL 420

Query: 421 FVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVW--ENIMQCDKGSDSKGIGAMLDEES 480
           FVPNTSVFNKVGTHGLPE NGQVDLSWQFH+RRVW  EN MQC KG+DSKG G +L EES
Sbjct: 421 FVPNTSVFNKVGTHGLPETNGQVDLSWQFHLRRVWEKENTMQCVKGNDSKGTGVVLQEES 480

Query: 481 EKPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           EK EVGGK+CENSTVFSSLPMAIKWLRDSVLQQNRS+RLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 EKSEVGGKNCENSTVFSSLPMAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Tan0015445 vs. NCBI nr
Match: XP_004142354.1 (folylpolyglutamate synthase [Cucumis sativus] >KGN52384.1 hypothetical protein Csa_008061 [Cucumis sativus])

HSP 1 Score: 962.6 bits (2487), Expect = 1.4e-276
Identity = 480/539 (89.05%), Postives = 508/539 (94.25%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEG +TLK S+ AYEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLEESMSQMKV
Sbjct: 16  MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKV 75

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRNCGF TGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW 
Sbjct: 76  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 135

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKE+TSED+PMP LFRFLALLAFKIFS+EQVDVAILEVGLGGTFDATNVVQAP VC
Sbjct: 136 CYNRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 195

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDHMELLG TLGEIASEKAGIFKRGAPAFTVFQPDEAMRVL+DKAS+LDVQLTV
Sbjct: 196 GIASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTV 255

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           VPSLDP+LLHGRKLGLEGEHQYLNA LA+ALSSTWLHR+GHS++SYVEQS SLPEQFIEG
Sbjct: 256 VPSLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEG 315

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQG-LNP 360
           LTTASFEGRAQIVPDRY D+E P++LVF+LDGAHSPESME CG WFSAA++ NQ G LN 
Sbjct: 316 LTTASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNH 375

Query: 361 QLEDN--GTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFN 420
           Q EDN  GTSSNK LQGYDNDEARKKSVQVLLFNCM VRDPQLLLPPLIKTCANNGV+F 
Sbjct: 376 QSEDNGSGTSSNK-LQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFK 435

Query: 421 KALFVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEE 480
           KALFVPNTSVFNKVGTHGLPE + QVDLSWQFHVRR+WE+  QCDKGSDSKG GA+L+EE
Sbjct: 436 KALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDKGSDSKGTGAVLEEE 495

Query: 481 SEKPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           SEK EVGGK+C NSTVFSSLP+AIKWLRDSVLQQNRS+RLQVLVTGSLHLVGDVLKLIK
Sbjct: 496 SEKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 553

BLAST of Tan0015445 vs. NCBI nr
Match: XP_022961882.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 962.2 bits (2486), Expect = 1.8e-276
Identity = 479/537 (89.20%), Postives = 501/537 (93.30%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           M EG   LK SRTAYEEALD LSSLIV+RRRCDKSGDRFEL+FEYVKMLDLEESMS+MKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLID+RERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKEKTSEDVPMP LFRFLALLAFKIF++EQVDVAILEVGLGGTFDATNVVQAP VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDH ELLG TLG IASEKAGIFKRGAPAFTV QPDEAM VLRDKAS+LDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           VPSLDP+LL G KLGLEGEHQYLNA LAIALSSTWLHRTGHS +SY EQSGSLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQG-LNP 360
           LTTASFEGRAQIVPDRY DIESPEDLVF+LDGAHSPESM VC SWF AAIK NQ+G L+ 
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKA 420
           QL+D GTSSNKLLQGYDNDEARKKSVQVLLFNCM+VRDPQLLL PLIKTC NNGVHF KA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 421 LFVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESE 480
           +FVPNTS+FNKVGTHGL EANG+VDLSWQFHVRRVWENI+Q +KG DS+GIGAM++EESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           KPEV GKSCENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Tan0015445 vs. ExPASy TrEMBL
Match: A0A6J1JQH1 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.2e-278
Identity = 484/537 (90.13%), Postives = 501/537 (93.30%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           M EG   LK SRTAYEEALD L SLIVNRRRCDKSGDRFELMFEYVKMLDLEESMS+MKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKEKTSEDVPMP LFRFLALLAFKIFS+EQVDVAILEVGLGGTFDATNVVQAP VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDH ELLG TLG IASEKAGIFKRGAPAFTVFQPDEAM VLRDKAS+LDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           VPSLDP+LL G KLGLEGEHQYLNA LAIALSSTWLHRTG S +SY EQSGSLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQG-LNP 360
           LTTA FEGRAQIVPDRY DIE+PEDLVF+LDGAHSPESM VC SWF AAIK NQ+G LN 
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 361 QLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKA 420
           QL+DNGTSSNKLLQGYDNDEARKKSVQVLLFNCM+VRDPQLLL PLIKTCANNGVHF KA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESE 480
           LFVPNTS+FNKVGTHGL EANG+VDLSWQFHVRRVWENI+Q +KG DS+GIGAM++EESE
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           KPEV GKSCENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Tan0015445 vs. ExPASy TrEMBL
Match: A0A0A0KTX7 (Folylpolyglutamate synthase OS=Cucumis sativus OX=3659 GN=Csa_5G630860 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.8e-277
Identity = 480/539 (89.05%), Postives = 508/539 (94.25%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           MVEG +TLK S+ AYEEALDALSSLIVNRRRCDKSGD+FELMFEYVKMLDLEESMSQMKV
Sbjct: 16  MVEGNETLKDSQMAYEEALDALSSLIVNRRRCDKSGDQFELMFEYVKMLDLEESMSQMKV 75

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRNCGF TGLFTSPHLIDIRERFRLDGIDISEEKFLTYFW 
Sbjct: 76  IHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWC 135

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKE+TSED+PMP LFRFLALLAFKIFS+EQVDVAILEVGLGGTFDATNVVQAP VC
Sbjct: 136 CYNRLKERTSEDIPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 195

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDHMELLG TLGEIASEKAGIFKRGAPAFTVFQPDEAMRVL+DKAS+LDVQLTV
Sbjct: 196 GIASLGYDHMELLGNTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLKDKASQLDVQLTV 255

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           VPSLDP+LLHGRKLGLEGEHQYLNA LA+ALSSTWLHR+GHS++SYVEQS SLPEQFIEG
Sbjct: 256 VPSLDPELLHGRKLGLEGEHQYLNAGLAVALSSTWLHRSGHSELSYVEQSRSLPEQFIEG 315

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQG-LNP 360
           LTTASFEGRAQIVPDRY D+E P++LVF+LDGAHSPESME CG WFSAA++ NQ G LN 
Sbjct: 316 LTTASFEGRAQIVPDRYTDVECPDELVFYLDGAHSPESMEACGRWFSAAVRHNQLGNLNH 375

Query: 361 QLEDN--GTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFN 420
           Q EDN  GTSSNK LQGYDNDEARKKSVQVLLFNCM VRDPQLLLPPLIKTCANNGV+F 
Sbjct: 376 QSEDNGSGTSSNK-LQGYDNDEARKKSVQVLLFNCMAVRDPQLLLPPLIKTCANNGVNFK 435

Query: 421 KALFVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEE 480
           KALFVPNTSVFNKVGTHGLPE + QVDLSWQFHVRR+WE+  QCDKGSDSKG GA+L+EE
Sbjct: 436 KALFVPNTSVFNKVGTHGLPETDEQVDLSWQFHVRRIWEDTKQCDKGSDSKGTGAVLEEE 495

Query: 481 SEKPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           SEK EVGGK+C NSTVFSSLP+AIKWLRDSVLQQNRS+RLQVLVTGSLHLVGDVLKLIK
Sbjct: 496 SEKSEVGGKNCGNSTVFSSLPLAIKWLRDSVLQQNRSVRLQVLVTGSLHLVGDVLKLIK 553

BLAST of Tan0015445 vs. ExPASy TrEMBL
Match: A0A6J1HBB1 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 8.9e-277
Identity = 479/537 (89.20%), Postives = 501/537 (93.30%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           M EG   LK SRTAYEEALD LSSLIV+RRRCDKSGDRFEL+FEYVKMLDLEESMS+MKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLID+RERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKEKTSEDVPMP LFRFLALLAFKIF++EQVDVAILEVGLGGTFDATNVVQAP VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDH ELLG TLG IASEKAGIFKRGAPAFTV QPDEAM VLRDKAS+LDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           VPSLDP+LL G KLGLEGEHQYLNA LAIALSSTWLHRTGHS +SY EQSGSLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQG-LNP 360
           LTTASFEGRAQIVPDRY DIESPEDLVF+LDGAHSPESM VC SWF AAIK NQ+G L+ 
Sbjct: 301 LTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDH 360

Query: 361 QLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKA 420
           QL+D GTSSNKLLQGYDNDEARKKSVQVLLFNCM+VRDPQLLL PLIKTC NNGVHF KA
Sbjct: 361 QLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCTNNGVHFKKA 420

Query: 421 LFVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESE 480
           +FVPNTS+FNKVGTHGL EANG+VDLSWQFHVRRVWENI+Q +KG DS+GIGAM++EESE
Sbjct: 421 VFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           KPEV GKSCENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Tan0015445 vs. ExPASy TrEMBL
Match: A0A6J1BXX6 (Folylpolyglutamate synthase OS=Momordica charantia OX=3673 GN=LOC111006595 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 5.8e-276
Identity = 475/537 (88.45%), Postives = 502/537 (93.48%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           M  G  TLK S+TAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEE M QMKV
Sbjct: 1   MENGNGTLKYSQTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEEPMLQMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKEKTS+D+PMPQLFRFLALLAFKIFS+EQVDVAILEVGLGGTFDATNVV+AP VC
Sbjct: 121 CYNRLKEKTSKDIPMPQLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVRAPIVC 180

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDHMELLG TLGEIASEKAGIFKRG PAFTV QPDEAM VLRDKASRLDVQLTV
Sbjct: 181 GIASLGYDHMELLGNTLGEIASEKAGIFKRGVPAFTVVQPDEAMNVLRDKASRLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           VPSLDP+LLHG KLGLEGEHQYLNA LAIALSS WL RTGHSK+SY+EQS SLPEQFIEG
Sbjct: 241 VPSLDPELLHGHKLGLEGEHQYLNAGLAIALSSAWLQRTGHSKLSYMEQSRSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQG-LNP 360
           LTTASFEGRAQIVPDRYIDIESP DLVF+LDGAHSPESME CG WFSA +K NQ+G LN 
Sbjct: 301 LTTASFEGRAQIVPDRYIDIESPGDLVFYLDGAHSPESMEACGRWFSAVMKDNQRGTLNY 360

Query: 361 QLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKA 420
           QLEDNG SSNKLLQ   NDEARK SV VLLFNCM+VRDPQLLLPPLIKTCANNGVHF KA
Sbjct: 361 QLEDNGISSNKLLQENQNDEARKTSVPVLLFNCMSVRDPQLLLPPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESE 480
           LFVPNTSVF+KVGTH L EANGQ+DLSWQF++RRVWEN++QC+KG+DSKG GA+L+EE++
Sbjct: 421 LFVPNTSVFDKVGTHALTEANGQIDLSWQFNIRRVWENLIQCEKGTDSKGTGAVLEEETD 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           KPEVGGKSCENSTVF SLP+AI+WLRDS+LQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVGGKSCENSTVFPSLPVAIRWLRDSILQQNRSIRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Tan0015445 vs. ExPASy TrEMBL
Match: A0A6J1JNC2 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 8.3e-275
Identity = 481/537 (89.57%), Postives = 497/537 (92.55%), Query Frame = 0

Query: 1   MVEGKDTLKCSRTAYEEALDALSSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSQMKV 60
           M EG   LK SRTAYEEALD L SLIVNRRRCDKSGDRFELMFEYVKMLDLEESMS+MKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 61  IHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWW 120
           IHVAGTKGKGSTCTFTESILR CGFHTGLFTSPHLIDIRERFRLDGIDISEEKFL YFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 121 CYNRLKEKTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVC 180
           CYNRLKEKTSEDVPMP LFRFLALLAFKIFS+EQVDVAILEVGLGGTFDATNVVQAP VC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 181 GIASLGYDHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTV 240
           GIASLGYDH ELLG TLG IASEKAGIFKRGAPAFTVFQPDEAM VLRDKAS+LDVQLTV
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDVQLTV 240

Query: 241 VPSLDPKLLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEG 300
           VPSLDP+LL G KLGLEGEHQYLNA LAIALSSTWLHRTG S +SY EQSGSLPEQFIEG
Sbjct: 241 VPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEG 300

Query: 301 LTTASFEGRAQIVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQG-LNP 360
           LTTA FEGRAQIVPDRY DIE+PEDLVF+LDGAHSPESM VC SWF AAIK NQ+G LN 
Sbjct: 301 LTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNH 360

Query: 361 QLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKA 420
           QL+DNGTSSNKLLQGYDNDEARKKSVQVLLFNCM+VRDPQLLL PLIKTCANNGVHF KA
Sbjct: 361 QLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKA 420

Query: 421 LFVPNTSVFNKVGTHGLPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESE 480
           LFVPNTS+FNKVGTHGL EANG+VDLSWQFHVRRVWENI+Q +     KGIGAM++EESE
Sbjct: 421 LFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSE-----KGIGAMVEEESE 480

Query: 481 KPEVGGKSCENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           KPEV GKSCENS VFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 481 KPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 532

BLAST of Tan0015445 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 640.2 bits (1650), Expect = 1.5e-183
Identity = 331/527 (62.81%), Postives = 398/527 (75.52%), Query Frame = 0

Query: 14  AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGS 73
           +YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + +M VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 74  TCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSE 133
           TCTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 134 DVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIASLGYDHME 193
           ++PMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN VQ P VCGI+SLGYDHME
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 194 LLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSLDPKLLHG 253
           +LG TLG+IA EKAGIFK G PAFTV QPDEAMRVL +KAS  +V L VV  L  +LL G
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 305

Query: 254 RKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEGLTTASFEGRAQ 313
           +KLGL+GEHQY+NA LA++L+S WL + G  ++    Q   LPE+FI+GL TAS +GRAQ
Sbjct: 306 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 365

Query: 314 IVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIK-RNQQGLNPQLEDNGTSSNK 373
           +VPD+Y +  +  DLVF+LDGAHSPESME C  WFS A+K  NQ G +  L +    S+ 
Sbjct: 366 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 425

Query: 374 LLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKALFVPNTSVFNK 433
               + N+       Q+LLFNCM+VRDP LLLP L   CA  GV+F KALFVPN SV++K
Sbjct: 426 --DKWSNETCE----QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHK 485

Query: 434 VGTHG-LPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESEKPEVGGKSCE 493
           VGT   LPE + QVDLSWQF +++VWE+++Q ++                  E  G+S  
Sbjct: 486 VGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESDG 545

Query: 494 NSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           NS VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 546 NSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 570

BLAST of Tan0015445 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 640.2 bits (1650), Expect = 1.5e-183
Identity = 331/527 (62.81%), Postives = 398/527 (75.52%), Query Frame = 0

Query: 14  AYEEALDALSSLIVNRRRCDKS--GDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGS 73
           +YEEAL ALSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + +M VIHVAGTKGKGS
Sbjct: 8   SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 67

Query: 74  TCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSE 133
           TCTFTESI+RN GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 68  TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 127

Query: 134 DVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIASLGYDHME 193
           ++PMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN VQ P VCGI+SLGYDHME
Sbjct: 128 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 187

Query: 194 LLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSLDPKLLHG 253
           +LG TLG+IA EKAGIFK G PAFTV QPDEAMRVL +KAS  +V L VV  L  +LL G
Sbjct: 188 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASETEVNLEVVQPLTARLLSG 247

Query: 254 RKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEGLTTASFEGRAQ 313
           +KLGL+GEHQY+NA LA++L+S WL + G  ++    Q   LPE+FI+GL TAS +GRAQ
Sbjct: 248 QKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPSRTQMSILPEKFIKGLATASLQGRAQ 307

Query: 314 IVPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIK-RNQQGLNPQLEDNGTSSNK 373
           +VPD+Y +  +  DLVF+LDGAHSPESME C  WFS A+K  NQ G +  L +    S+ 
Sbjct: 308 VVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWFSVAVKGDNQSGSSGHLVNGSAGSSH 367

Query: 374 LLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKALFVPNTSVFNK 433
               + N+       Q+LLFNCM+VRDP LLLP L   CA  GV+F KALFVPN SV++K
Sbjct: 368 --DKWSNETCE----QILLFNCMSVRDPNLLLPHLKNMCAKYGVNFKKALFVPNMSVYHK 427

Query: 434 VGTHG-LPEANGQVDLSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESEKPEVGGKSCE 493
           VGT   LPE + QVDLSWQF +++VWE+++Q ++                  E  G+S  
Sbjct: 428 VGTAADLPENDPQVDLSWQFTLQKVWESLVQSERDG----------------EKDGESDG 487

Query: 494 NSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
           NS VF+SLPMAIK LRD+V + + + R QVLVTGSLHLVGDVL+LI+
Sbjct: 488 NSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTGSLHLVGDVLRLIR 512

BLAST of Tan0015445 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 573.2 bits (1476), Expect = 2.2e-163
Identity = 301/525 (57.33%), Postives = 376/525 (71.62%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LE+++SQMK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSED 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 VPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIASLGYDHMEL 194
           VPMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV+Q P VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 195 LGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSLDPKLLHGR 254
           LGYTL EIA+EKAGIFK G PAFTV QPDEAMRVL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNA LA+AL ST+L   G    + ++Q+  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQGLNPQLEDNGTSSNKLL 374
           VPD     E PE++V++LDGAHSPESME C  WFS  IK+NQ                  
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ------------------ 372

Query: 375 QGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKALFVPNTSVFNKVG 434
                +  +K+S Q+LLFNCM+VRDP LLLP L   C + GV F +A+FVPN SV+N+VG
Sbjct: 373 -----ERNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVG 432

Query: 435 THGLPEANGQVD-LSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESEKPEVGGKSCENS 494
           +        +V+ +SWQF ++R+WE++ + +  S+SK             +  GK  E S
Sbjct: 433 SS--TNVGTRVESMSWQFGLQRIWESLARGEAKSNSKS------------DSKGKEEEKS 491

Query: 495 TVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 491

BLAST of Tan0015445 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 567.4 bits (1461), Expect = 1.2e-161
Identity = 300/525 (57.14%), Postives = 375/525 (71.43%), Query Frame = 0

Query: 15  YEEALDALSSLIVNRRR--CDKSGDRFELMFEYVKMLDLEESMSQMKVIHVAGTKGKGST 74
           Y+  LDALSSLI  R R   +    RF L+F Y+K+L+LE+++SQMK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 75  CTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKEKTSED 134
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 135 VPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIASLGYDHMEL 194
           VPMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATN +Q P VCGI+SLGYDHME+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATN-IQKPVVCGISSLGYDHMEI 192

Query: 195 LGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSLDPKLLHGR 254
           LGYTL EIA+EKAGIFK G PAFTV QPDEAMRVL +KAS+L+V L VV  LD      +
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKLEVNLQVVEPLD----SSQ 252

Query: 255 KLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQSGSLPEQFIEGLTTASFEGRAQI 314
           +LGL+GEHQYLNA LA+AL ST+L   G    + ++Q+  LPE+FI GL+ A   GRA I
Sbjct: 253 RLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLDQTNGLPEKFISGLSNAYLMGRAMI 312

Query: 315 VPDRYIDIESPEDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQGLNPQLEDNGTSSNKLL 374
           VPD     E PE++V++LDGAHSPESME C  WFS  IK+NQ                  
Sbjct: 313 VPDS----ELPEEIVYYLDGAHSPESMEACAIWFSKQIKQNQ------------------ 372

Query: 375 QGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFNKALFVPNTSVFNKVG 434
                +  +K+S Q+LLFNCM+VRDP LLLP L   C + GV F +A+FVPN SV+N+VG
Sbjct: 373 -----ERNQKRSEQILLFNCMSVRDPSLLLPRLRSKCIDQGVDFKRAVFVPNVSVYNQVG 432

Query: 435 THGLPEANGQVD-LSWQFHVRRVWENIMQCDKGSDSKGIGAMLDEESEKPEVGGKSCENS 494
           +        +V+ +SWQF ++R+WE++ + +  S+SK             +  GK  E S
Sbjct: 433 SS--TNVGTRVESMSWQFGLQRIWESLARGEAKSNSKS------------DSKGKEEEKS 490

Query: 495 TVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 537
            VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHLVGD+L+ IK
Sbjct: 493 FVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHLVGDLLRFIK 490

BLAST of Tan0015445 vs. TAIR 10
Match: AT3G10160.1 (DHFS-FPGS homolog C )

HSP 1 Score: 534.6 bits (1376), Expect = 8.8e-152
Identity = 288/552 (52.17%), Postives = 380/552 (68.84%), Query Frame = 0

Query: 13  TAYEEALDALSSLIVNRRRCDK-----SGDRFELMFEYVKMLDLEESMSQMKVIHVAGTK 72
           ++Y++A++ALS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 73  GKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLTYFWWCYNRLKE 132
           GKGSTC F+E+ILRNCGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 133 KTSEDVPMPQLFRFLALLAFKIFSEEQVDVAILEVGLGGTFDATNVVQAPTVCGIASLGY 192
           K  + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV+Q P VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 193 DHMELLGYTLGEIASEKAGIFKRGAPAFTVFQPDEAMRVLRDKASRLDVQLTVVPSLDPK 252
           DHM++LG TL +IA  KAGIFK   PAFTV Q  EAM VL+  A+ L+V L VV  L+PK
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNLEVPLEVVAPLEPK 320

Query: 253 LLHGRKLGLEGEHQYLNASLAIALSSTWLHRTGHSKMSYVEQS--GSLPEQFIEGLTTAS 312
            L G  LGL G+HQ +NA LA++LS  WL RTG+ K  +  +S    +P  F  GL TA 
Sbjct: 321 KLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNWKKIFPNESKETEIPVAFCRGLATAR 380

Query: 313 FEGRAQIVPDRYID-------IESP-EDLVFFLDGAHSPESMEVCGSWFSAAIKRNQQGL 372
             GRAQ+V D   D       +E+P  DL+F+LDGAHSPESME CG WFS+A+ R  + L
Sbjct: 381 LHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDGAHSPESMEACGRWFSSAV-RGDKSL 440

Query: 373 NPQLEDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMTVRDPQLLLPPLIKTCANNGVHFN 432
           +  +      +  +  G    +  + S Q+LLFNCM VRDPQ+LLP L+ TCA++G HF+
Sbjct: 441 STAV------NGYMRHGEYGTDLNRVSKQILLFNCMEVRDPQVLLPKLVTTCASSGTHFS 500

Query: 433 KALFVPNTSVFNKV--GTHGLPEANGQVDLSWQFHVRRVWENIMQ-CDKGSD----SKGI 492
           +ALFVP+ S +NKV  G   +P    + DL+WQF ++R+WE  +Q  D G D      GI
Sbjct: 501 RALFVPSMSTYNKVISGASAIPSDTRRKDLTWQFRLQRLWEKSIQGTDAGLDHTLKPDGI 560

Query: 493 GAMLDEE---SEKPEVGGKS---CENSTVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGS 537
            A+   +    + P+ GG +     +S V  SLP+ I WLRD V ++N S++L+VLVTGS
Sbjct: 561 TALPPHDFLCGDAPQCGGPAGTPVTSSAVMPSLPLTINWLRDCV-RRNPSLKLEVLVTGS 620