Tan0015425 (gene) Snake gourd v1

BLAST of Tan0015425 vs. ExPASy Swiss-Prot
Match: C8VK14 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=hxnY PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.4e-43
Identity = 114/320 (35.62%), Postives = 170/320 (53.12%), Query Frame = 0

Query: 30  VSLLKQACLETGFFYAVNHGITEEFMNEVFTQSKKLFSLPLSDKMKLLR--NEKHRGYTP 89
           V  ++++C   GFF    H I  E    V   +K+ F+LPL +KM + +  N  +RGY  
Sbjct: 26  VQQVRESCEYNGFFQITGHRIPRELQVRVMDAAKRFFALPLEEKMAIDKNLNSFNRGYEL 85

Query: 90  MLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDSDGEWPFY-------GPNIWPEEDLLPG 149
           +  ++L+       + KEG YIG  E+I  D      P+Y       GPN WP    +P 
Sbjct: 86  LRSQMLEVGT--APELKEGLYIG--EEIGADH-----PYYINGRLNSGPNQWPA--TVPD 145

Query: 150 ---WREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEMLGEPIAILRLLHYEGQ-I 209
              +RE   +++    E+AK +  ++A+ L +E  FFD     G  +A +R+LHY  Q  
Sbjct: 146 AQEFRETSMEYYHAVYELAKDVLAVLALTLDVEESFFDPLTEGG--VATMRMLHYPSQPK 205

Query: 210 SEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWEYVAPLKGAFVVNLGDM 269
            E  K   G GAH+D+G ITLL  DE  GLQ+    DA    W  V P+ GA+VVNLGD+
Sbjct: 206 DEDEKLNRGIGAHTDFGCITLLLQDEVDGLQVL---DAPSGQWLDVQPVLGAYVVNLGDL 265

Query: 270 LERWSNCIFKSTLHRVL-INGQERYSIAYFVEPSHDCLIECLPTCKSDKNPPKFPPILCK 329
           + R +N  +KS +HRV+  +G+ERYSI +F   + D + +CLP C      PK+PPI  +
Sbjct: 266 MMRMANDRYKSNIHRVINKSGRERYSIPFFFSGNPDHVCKCLPNCCKAGEQPKYPPITVE 325

Query: 330 TYLSQRYNETHVDLNTYKKD 336
             +   Y +++     YKK+
Sbjct: 326 DMVRGAYKQSYGRAEAYKKE 329

BLAST of Tan0015425 vs. ExPASy Swiss-Prot
Match: Q6K332 (2-oxoglutarate-dependent dioxygenase 33 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD33 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.4e-36
Identity = 96/288 (33.33%), Postives = 151/288 (52.43%), Query Frame = 0

Query: 21  LSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFTQSKKLFSLPLSDKMKLLRNE 80
           ++  D+ Q V +L  AC E GFFY   HGI +  M +V   ++K F LP  +K+K+    
Sbjct: 26  VNDEDLLQVVQMLDDACREAGFFYVKGHGIADSLMKQVRDVTQKFFQLPYEEKLKIKMTP 85

Query: 81  K--HRGYTPMLDELL--DPDNQIRGDYKEGYYIGIEEKIPGDDSDGEWPFYGPNIWPEED 140
           +  +RGY  + + +    PD     D     Y  IE   PG   D   P  G N+WP+  
Sbjct: 86  QSGYRGYQRVGENITKGKPDMHEAID----CYTPIE---PGKYGDLAKPMVGSNLWPKYP 145

Query: 141 LLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEMLGEPIAILRLLHY--EG 200
               +  ++E +     ++++ I R IA+ALG   D F+     G+P  + RL+ Y    
Sbjct: 146 --SNFDVLLENYISLLRDLSRKIMRGIALALGAPVDAFE-GTTAGDPFWVCRLIGYPVST 205

Query: 201 QISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWEYVAPLKGAFVVNLG 260
            I E  +   G GAH+DYGL+TL+  D+++     +++  +   W Y  P+ G FV N+G
Sbjct: 206 DIPEEQRTDTGCGAHTDYGLLTLVNQDDDICALEVRNQSGE---WIYAKPIPGTFVCNIG 265

Query: 261 DMLERWSNCIFKSTLHRVLING-QERYSIAYFVEPSHDCLIECLPTCK 302
           DML+ WSN I++ TLHRV+ N  + R S+A+F E + D  +E +  C+
Sbjct: 266 DMLKVWSNGIYQPTLHRVVNNSPRYRVSVAFFYESNFDAAVEPVEFCR 300

BLAST of Tan0015425 vs. ExPASy Swiss-Prot
Match: Q1ERI0 (2-oxoglutarate-dependent dioxygenase mpl2 OS=Monascus purpureus OX=5098 GN=mpl2 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.2e-35
Identity = 98/279 (35.13%), Postives = 149/279 (53.41%), Query Frame = 0

Query: 20  DLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFTQSKKLFSLPLSDKMKLLRN 79
           D +S    + +  ++ AC  TGFF  + HGI+      VF  + K F+LP SD     RN
Sbjct: 22  DPNSDAARKVIDDVRAACTSTGFFQLLGHGISPALQQSVFAAAAKFFALP-SDVKSRCRN 81

Query: 80  EKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS--DGEWPFYGPNIWPEEDL 139
              RGY PM  +  +    +  D KEG+  G  + IP DD     +  F G N WP  +L
Sbjct: 82  VGFRGYDPMASQSYELG--VLPDLKEGFIAG--KDIPLDDPRVASQRFFMGQNAWPPSEL 141

Query: 140 LP--GWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEMLGEPIAILRLLHY-EG 199
           LP   +R  +E++++  L++   +  L+A  L      FD+ +   +P   LRLLHY   
Sbjct: 142 LPEANFRRPIEEYYQAMLKLCWVVLDLVAATLPYGPHVFDEFKE-NDPACPLRLLHYPPA 201

Query: 200 QISEPAKG-IFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWEYVAPLKGAFVVNL 259
              + AKG   G+ AH+D+G ITLL  D++ GL++   +D +   W  V P K A+VVNL
Sbjct: 202 PAPDVAKGRQLGSSAHTDFGAITLLLQDDHSGLEV---QDCETGEWIGVPPNKDAYVVNL 261

Query: 260 GDMLERWSNCIFKSTLHRVL-INGQERYSIAYFVEPSHD 292
           GDM+ R +   +KS++HRV+  N  +RYS+ +F + + D
Sbjct: 262 GDMMSRITRGHYKSSIHRVINQNLTDRYSVVFFFDGNLD 291

BLAST of Tan0015425 vs. ExPASy Swiss-Prot
Match: A0A159BP93 (2-oxoglutarate-dependent dioxygenase citB OS=Monascus ruber OX=89489 GN=citB PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.2e-35
Identity = 98/279 (35.13%), Postives = 149/279 (53.41%), Query Frame = 0

Query: 20  DLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFTQSKKLFSLPLSDKMKLLRN 79
           D +S    + +  ++ AC  TGFF  + HGI+      VF  + K F+LP SD     RN
Sbjct: 22  DPNSDAARKVIDDVRAACTSTGFFQLLGHGISPALQQSVFAAAAKFFALP-SDVKSRCRN 81

Query: 80  EKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS--DGEWPFYGPNIWPEEDL 139
              RGY PM  +  +    +  D KEG+  G  + IP DD     +  F G N WP  +L
Sbjct: 82  VGFRGYDPMASQSYELG--VLPDLKEGFIAG--KDIPLDDPRVASQRFFMGQNAWPPSEL 141

Query: 140 LP--GWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEMLGEPIAILRLLHY-EG 199
           LP   +R  +E++++  L++   +  L+A  L      FD+ +   +P   LRLLHY   
Sbjct: 142 LPEANFRRPIEEYYQAMLKLCWVVLDLVAATLPYGPHVFDEFKE-NDPACPLRLLHYPPA 201

Query: 200 QISEPAKG-IFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWEYVAPLKGAFVVNL 259
              + AKG   G+ AH+D+G ITLL  D++ GL++   +D +   W  V P K A+VVNL
Sbjct: 202 PAPDVAKGRQLGSSAHTDFGAITLLLQDDHSGLEV---QDCETGEWIGVPPNKDAYVVNL 261

Query: 260 GDMLERWSNCIFKSTLHRVL-INGQERYSIAYFVEPSHD 292
           GDM+ R +   +KS++HRV+  N  +RYS+ +F + + D
Sbjct: 262 GDMMSRITRGHYKSSIHRVINQNLTDRYSVVFFFDGNLD 291

BLAST of Tan0015425 vs. ExPASy Swiss-Prot
Match: Q84MB6 (Probable 2-oxoglutarate-dependent dioxygenase At3g50210 OS=Arabidopsis thaliana OX=3702 GN=At3g50210 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.6e-34
Identity = 98/306 (32.03%), Postives = 163/306 (53.27%), Query Frame = 0

Query: 16  LNCIDLSSSD---IHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFTQSKKLFSLPLSD 75
           L C D   ++   + + V  L +AC + GFFY + HGI+E+ +N+V   +++ F LP  +
Sbjct: 18  LKCDDPDMAEDVGVAEVVQQLDKACRDAGFFYVIGHGISEDVINKVREITREFFKLPYEE 77

Query: 76  KMKLLRNEK--HRGY----------TPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 135
           K+K+       +RGY           P + E +D   +I    K+G Y  I + +     
Sbjct: 78  KLKIKMTPAAGYRGYQRIGENVTKGIPDIHEAIDCYREI----KQGKYGDIGKVME---- 137

Query: 136 DGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVAL-GLENDFFDKSEM 195
                  GPN WPE      ++E+ME++ +   ++++ I R I++AL G   +F  + +M
Sbjct: 138 -------GPNQWPENP--QEFKELMEEYIKLCTDLSRKILRGISLALAGSPYEF--EGKM 197

Query: 196 LGEPIAILRLLHYEG---QISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKP 255
            G+P  ++RL+ Y G      +P   I G GAH+DYGL+TL+  D++      ++   + 
Sbjct: 198 AGDPFWVMRLIGYPGAEFTNGQPENDI-GCGAHTDYGLLTLVNQDDDKTALQVRNLGGE- 257

Query: 256 QNWEYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLING-QERYSIAYFVEPSHDCLIE 302
             W    P+ G+FV N+GDML+  SN +++STLHRV+ N  Q R  +A+F E + D ++E
Sbjct: 258 --WISAIPIPGSFVCNIGDMLKILSNGVYESTLHRVINNSPQYRVCVAFFYETNFDAVVE 300

BLAST of Tan0015425 vs. NCBI nr
Match: XP_023545999.1 (2-oxoglutarate-dependent dioxygenase mpl2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 638.3 bits (1645), Expect = 3.9e-179
Identity = 300/338 (88.76%), Postives = 321/338 (94.97%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MEN   ++PNP FSALNCIDLSSSDIH SVSLLKQACL+TGFFYAVNHGI EEFM+EVF 
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120
           QSK+LFSLPLSDKMKLLRNEKHRGYTP+LDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120

Query: 121 DGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEML 180
            GE PFYGPNIWP EDLLPGWR+IMEKFHR+ALEVA+AIARLIA+ALGLENDFFDKSEML
Sbjct: 121 GGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEML 180

Query: 181 GEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWE 240
           GEPIA+LRLLHYEGQISEPAKGIFGAGAH+DYGLITLLATDEN GLQICKDK+AKPQ WE
Sbjct: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQIWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTC 300
           YVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQ+RYSIAYFVEPSHDCL+ECLPTC
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPSHDCLVECLPTC 300

Query: 301 KSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKKDQEQ 339
           KSD+NPPKFPPILCKTYLSQRY ETHVDL+TYK   ++
Sbjct: 301 KSDENPPKFPPILCKTYLSQRYKETHVDLDTYKSSNDE 338

BLAST of Tan0015425 vs. NCBI nr
Match: XP_038877416.1 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY-like isoform X2 [Benincasa hispida])

HSP 1 Score: 637.5 bits (1643), Expect = 6.6e-179
Identity = 298/334 (89.22%), Postives = 320/334 (95.81%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MENP E++ NPKF ALNCIDLSSSD+++SVSLLKQACL+TGFFYAVNHGITEEFM+EVF 
Sbjct: 28  MENPTENDSNPKFLALNCIDLSSSDVNRSVSLLKQACLDTGFFYAVNHGITEEFMDEVFA 87

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120
           QSK+LFSLPLSDKMKLLRNEKHRGYTP+LDELLDPDNQIRGDYKEGYYIGIEEK PGDDS
Sbjct: 88  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGIEEKNPGDDS 147

Query: 121 DGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEML 180
           DGE PFYGPNIWP EDLLPGWR+IMEKFH QALEVA+AIARLIA+AL LENDFFDK+E L
Sbjct: 148 DGERPFYGPNIWPAEDLLPGWRKIMEKFHTQALEVARAIARLIALALDLENDFFDKAETL 207

Query: 181 GEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWE 240
           GEPIAILRLLHYEGQ+SEPAKGIFGAGAH+DYGLITLLATDENLGLQICKDKDAKPQ W+
Sbjct: 208 GEPIAILRLLHYEGQVSEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQIWQ 267

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTC 300
           YVAPLKG FVVNLGDMLERWSNCIFKSTLHRVL+NGQ+RYSIAYFVEP+HDCL+ECLPTC
Sbjct: 268 YVAPLKGTFVVNLGDMLERWSNCIFKSTLHRVLVNGQDRYSIAYFVEPNHDCLVECLPTC 327

Query: 301 KSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKK 335
           KSDKNPPKFPPI C+TYLSQRY ETHVDL+TYKK
Sbjct: 328 KSDKNPPKFPPIRCRTYLSQRYKETHVDLSTYKK 361

BLAST of Tan0015425 vs. NCBI nr
Match: KAG7030452.1 (encD [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 636.7 bits (1641), Expect = 1.1e-178
Identity = 299/338 (88.46%), Postives = 321/338 (94.97%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MEN   ++PNP FSALNCIDLSSSDIH SVSLLKQACL+TGFFYAVNHGI EEFM+EVF 
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120
           QSK+LFSLPLSDKMKLLRNEKHRGYTP+LDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120

Query: 121 DGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEML 180
            GE PFYGPNIWP EDLLPGWR+IMEKFHR+ALEVA+AIARLIA+ALGLENDFFDKSEML
Sbjct: 121 GGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEML 180

Query: 181 GEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWE 240
           GEPIA+LRLLHYEG+ISEPAKGIFGAGAH+DYGLITLLATDEN GLQICKDK+AKPQ WE
Sbjct: 181 GEPIAVLRLLHYEGKISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQVWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTC 300
           YVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQ+RYSIAYFVEPSHDCL+ECLPTC
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPSHDCLVECLPTC 300

Query: 301 KSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKKDQEQ 339
           KSD+NPPKFPPILCKTYLSQRY ETHVDL+TYK   ++
Sbjct: 301 KSDENPPKFPPILCKTYLSQRYKETHVDLDTYKSSNDE 338

BLAST of Tan0015425 vs. NCBI nr
Match: XP_022946400.1 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 634.4 bits (1635), Expect = 5.6e-178
Identity = 298/338 (88.17%), Postives = 320/338 (94.67%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MEN   ++P+P FSALNCIDLSSSDIH SVSLLKQACL+TGFFYAVNHGI EEFM+EVF 
Sbjct: 1   MENRTVNDPSPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120
           QSK+LFSLPLSDKMKLLRNEKHRGYTP+LDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120

Query: 121 DGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEML 180
            GE PFYGPNIWP EDLLPGWR+IMEKFHR+ALEVA+AIARLIA+ALGLENDFFDKSEML
Sbjct: 121 GGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEML 180

Query: 181 GEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWE 240
           GEPIA+LRLLHYEGQISEPAKGIFGAGAH+DYGLITLLATDEN GLQICKDK+AKPQ WE
Sbjct: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQVWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTC 300
           YVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQ+RYSIAYFVEP HDCL+ECLPTC
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPRHDCLVECLPTC 300

Query: 301 KSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKKDQEQ 339
           KSD+NPPKFPPILCKTYLSQRY ETHVDL+TYK   ++
Sbjct: 301 KSDENPPKFPPILCKTYLSQRYKETHVDLDTYKSSNDE 338

BLAST of Tan0015425 vs. NCBI nr
Match: XP_023545997.1 (2-oxoglutarate-dependent dioxygenase mpl2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545998.1 2-oxoglutarate-dependent dioxygenase mpl2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 633.6 bits (1633), Expect = 9.5e-178
Identity = 300/339 (88.50%), Postives = 321/339 (94.69%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MEN   ++PNP FSALNCIDLSSSDIH SVSLLKQACL+TGFFYAVNHGI EEFM+EVF 
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIR-GDYKEGYYIGIEEKIPGDD 120
           QSK+LFSLPLSDKMKLLRNEKHRGYTP+LDELLDPDNQIR GDYKEGYYIGIEEKIPGDD
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRVGDYKEGYYIGIEEKIPGDD 120

Query: 121 SDGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEM 180
           S GE PFYGPNIWP EDLLPGWR+IMEKFHR+ALEVA+AIARLIA+ALGLENDFFDKSEM
Sbjct: 121 SGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEM 180

Query: 181 LGEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNW 240
           LGEPIA+LRLLHYEGQISEPAKGIFGAGAH+DYGLITLLATDEN GLQICKDK+AKPQ W
Sbjct: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQIW 240

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPT 300
           EYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQ+RYSIAYFVEPSHDCL+ECLPT
Sbjct: 241 EYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPSHDCLVECLPT 300

Query: 301 CKSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKKDQEQ 339
           CKSD+NPPKFPPILCKTYLSQRY ETHVDL+TYK   ++
Sbjct: 301 CKSDENPPKFPPILCKTYLSQRYKETHVDLDTYKSSNDE 339

BLAST of Tan0015425 vs. ExPASy TrEMBL
Match: A0A6J1G3R7 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450469 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.7e-178
Identity = 298/338 (88.17%), Postives = 320/338 (94.67%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MEN   ++P+P FSALNCIDLSSSDIH SVSLLKQACL+TGFFYAVNHGI EEFM+EVF 
Sbjct: 1   MENRTVNDPSPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120
           QSK+LFSLPLSDKMKLLRNEKHRGYTP+LDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120

Query: 121 DGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEML 180
            GE PFYGPNIWP EDLLPGWR+IMEKFHR+ALEVA+AIARLIA+ALGLENDFFDKSEML
Sbjct: 121 GGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEML 180

Query: 181 GEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWE 240
           GEPIA+LRLLHYEGQISEPAKGIFGAGAH+DYGLITLLATDEN GLQICKDK+AKPQ WE
Sbjct: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQVWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTC 300
           YVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQ+RYSIAYFVEP HDCL+ECLPTC
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPRHDCLVECLPTC 300

Query: 301 KSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKKDQEQ 339
           KSD+NPPKFPPILCKTYLSQRY ETHVDL+TYK   ++
Sbjct: 301 KSDENPPKFPPILCKTYLSQRYKETHVDLDTYKSSNDE 338

BLAST of Tan0015425 vs. ExPASy TrEMBL
Match: A0A6J1G3P3 (2-oxoglutarate-dependent dioxygenase mpl2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450469 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 6.6e-177
Identity = 298/339 (87.91%), Postives = 320/339 (94.40%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MEN   ++P+P FSALNCIDLSSSDIH SVSLLKQACL+TGFFYAVNHGI EEFM+EVF 
Sbjct: 1   MENRTVNDPSPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIR-GDYKEGYYIGIEEKIPGDD 120
           QSK+LFSLPLSDKMKLLRNEKHRGYTP+LDELLDPDNQIR GDYKEGYYIGIEEKIPGDD
Sbjct: 61  QSKRLFSLPLSDKMKLLRNEKHRGYTPILDELLDPDNQIRVGDYKEGYYIGIEEKIPGDD 120

Query: 121 SDGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEM 180
           S GE PFYGPNIWP EDLLPGWR+IMEKFHR+ALEVA+AIARLIA+ALGLENDFFDKSEM
Sbjct: 121 SGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEM 180

Query: 181 LGEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNW 240
           LGEPIA+LRLLHYEGQISEPAKGIFGAGAH+DYGLITLLATDEN GLQICKDK+AKPQ W
Sbjct: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKEAKPQVW 240

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPT 300
           EYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQ+RYSIAYFVEP HDCL+ECLPT
Sbjct: 241 EYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPRHDCLVECLPT 300

Query: 301 CKSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKKDQEQ 339
           CKSD+NPPKFPPILCKTYLSQRY ETHVDL+TYK   ++
Sbjct: 301 CKSDENPPKFPPILCKTYLSQRYKETHVDLDTYKSSNDE 339

BLAST of Tan0015425 vs. ExPASy TrEMBL
Match: A0A6J1KDA3 (2-oxoglutarate-Fe(II) type oxidoreductase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493849 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 1.9e-176
Identity = 296/338 (87.57%), Postives = 319/338 (94.38%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MEN   ++PNP FSALNCIDLSSSDIH SVSLLKQACL+TGFFYAVNHGI EEFM+EVF 
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120
           QSK+LFSLPL DKMKLLRNEKHRGYTP+LDELLDP+NQIRGDYKEGYYIGIEEK PGDDS
Sbjct: 61  QSKRLFSLPLCDKMKLLRNEKHRGYTPILDELLDPENQIRGDYKEGYYIGIEEKNPGDDS 120

Query: 121 DGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEML 180
            GE PFYGPNIWP EDLLPGWR+IMEKFHR+ALEVA+AIARLIA+ALGLENDFFDKSEML
Sbjct: 121 GGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEML 180

Query: 181 GEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWE 240
           GEPIA+LRLLHYEGQISEPAKGIFGAGAH+DYGLITLLATDEN GLQICKDKDAKP+ WE
Sbjct: 181 GEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKDAKPRIWE 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTC 300
           YVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQ+RYSIAYFVEPS+DCL+ECLPTC
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPSNDCLVECLPTC 300

Query: 301 KSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKKDQEQ 339
           KSD+NPPKFPPILCKTYLSQRY ETHVDL+TYK   ++
Sbjct: 301 KSDENPPKFPPILCKTYLSQRYKETHVDLDTYKSSIDE 338

BLAST of Tan0015425 vs. ExPASy TrEMBL
Match: A0A6J1KFK8 (2-oxoglutarate-Fe(II) type oxidoreductase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493849 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 4.8e-175
Identity = 296/339 (87.32%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MEN   ++PNP FSALNCIDLSSSDIH SVSLLKQACL+TGFFYAVNHGI EEFM+EVF 
Sbjct: 1   MENRTVNDPNPIFSALNCIDLSSSDIHLSVSLLKQACLDTGFFYAVNHGIPEEFMDEVFA 60

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIR-GDYKEGYYIGIEEKIPGDD 120
           QSK+LFSLPL DKMKLLRNEKHRGYTP+LDELLDP+NQIR GDYKEGYYIGIEEK PGDD
Sbjct: 61  QSKRLFSLPLCDKMKLLRNEKHRGYTPILDELLDPENQIRVGDYKEGYYIGIEEKNPGDD 120

Query: 121 SDGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEM 180
           S GE PFYGPNIWP EDLLPGWR+IMEKFHR+ALEVA+AIARLIA+ALGLENDFFDKSEM
Sbjct: 121 SGGEMPFYGPNIWPAEDLLPGWRKIMEKFHREALEVARAIARLIALALGLENDFFDKSEM 180

Query: 181 LGEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNW 240
           LGEPIA+LRLLHYEGQISEPAKGIFGAGAH+DYGLITLLATDEN GLQICKDKDAKP+ W
Sbjct: 181 LGEPIAVLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENFGLQICKDKDAKPRIW 240

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPT 300
           EYVAPLKGAFVVNLGDMLERWSNC+F+STLHRVL NGQ+RYSIAYFVEPS+DCL+ECLPT
Sbjct: 241 EYVAPLKGAFVVNLGDMLERWSNCVFRSTLHRVLGNGQDRYSIAYFVEPSNDCLVECLPT 300

Query: 301 CKSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKKDQEQ 339
           CKSD+NPPKFPPILCKTYLSQRY ETHVDL+TYK   ++
Sbjct: 301 CKSDENPPKFPPILCKTYLSQRYKETHVDLDTYKSSIDE 339

BLAST of Tan0015425 vs. ExPASy TrEMBL
Match: A0A0A0LIR5 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000940 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.6e-170
Identity = 283/334 (84.73%), Postives = 309/334 (92.51%), Query Frame = 0

Query: 1   MENPAESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFT 60
           MENP E++PNP+F ALNCIDLSSSD++ SVSLLKQACL+TGFFY VNHGITEEFM+EVF 
Sbjct: 1   MENPTENDPNPEFLALNCIDLSSSDVNLSVSLLKQACLDTGFFYVVNHGITEEFMDEVFA 60

Query: 61  QSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDS 120
           QSK+LF LPLSDKMKLLRNE HRGYTP+LDELLDP+NQIRGDYKEGYYIGIEE  PGD S
Sbjct: 61  QSKRLFDLPLSDKMKLLRNENHRGYTPILDELLDPENQIRGDYKEGYYIGIEETNPGDGS 120

Query: 121 DGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEML 180
           DG+ PFYGPN+WP ED+LP W +IMEKFH QALEVA+ +ARLIA+ALGLE +FFDKSEML
Sbjct: 121 DGKRPFYGPNVWPAEDMLPDWSKIMEKFHAQALEVARVVARLIALALGLEKEFFDKSEML 180

Query: 181 GEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWE 240
           G+P A LRLLHYEGQISEPAKGIFGAGAH+DYGLITLLATDENLGLQICKDKDAKPQ W+
Sbjct: 181 GKPTATLRLLHYEGQISEPAKGIFGAGAHTDYGLITLLATDENLGLQICKDKDAKPQVWQ 240

Query: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTC 300
           YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVL++GQ+RYSIAYFVEP+ DCL+ECLPTC
Sbjct: 241 YVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLVSGQDRYSIAYFVEPNQDCLVECLPTC 300

Query: 301 KSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKK 335
           KSDKNPPKFPPI C TYLSQRY ETHVDL TYKK
Sbjct: 301 KSDKNPPKFPPIRCGTYLSQRYKETHVDLETYKK 334

BLAST of Tan0015425 vs. TAIR 10
Match: AT1G35190.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 488.4 bits (1256), Expect = 4.6e-138
Identity = 224/327 (68.50%), Postives = 276/327 (84.40%), Query Frame = 0

Query: 6   ESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFTQSKKL 65
           E++   K S+LNCIDL++ D++ SV  LKQACL+ GFFY +NHGI+EEFM++VF QSKKL
Sbjct: 2   ENHTTMKVSSLNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFEQSKKL 61

Query: 66  FSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDSDGEWP 125
           F+LPL +KMK+LRNEKHRGYTP+LDELLDP NQI GD+KEGYYIGIE  +P DD   + P
Sbjct: 62  FALPLEEKMKVLRNEKHRGYTPVLDELLDPKNQINGDHKEGYYIGIE--VPKDDPHWDKP 121

Query: 126 FYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEMLGEPIA 185
           FYGPN WP+ D+LPGWRE MEK+H++AL V+ AIARL+A+AL L+  +FD++EMLG+PIA
Sbjct: 122 FYGPNPWPDADVLPGWRETMEKYHQEALRVSMAIARLLALALDLDVGYFDRTEMLGKPIA 181

Query: 186 ILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWEYVAPL 245
            +RLL Y+G IS+P+KGI+  GAHSD+G++TLLATD  +GLQICKDK+A PQ WEYV P+
Sbjct: 182 TMRLLRYQG-ISDPSKGIYACGAHSDFGMMTLLATDGVMGLQICKDKNAMPQKWEYVPPI 241

Query: 246 KGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTCKSDKN 305
           KGAF+VNLGDMLERWSN  FKSTLHRVL NGQERYSI +FVEP+HDCL+ECLPTCKS+  
Sbjct: 242 KGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHDCLVECLPTCKSESE 301

Query: 306 PPKFPPILCKTYLSQRYNETHVDLNTY 333
            PK+PPI C TYL+QRY ETH +L+ Y
Sbjct: 302 LPKYPPIKCSTYLTQRYEETHANLSIY 325

BLAST of Tan0015425 vs. TAIR 10
Match: AT1G35190.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 2.8e-127
Identity = 212/327 (64.83%), Postives = 258/327 (78.90%), Query Frame = 0

Query: 6   ESNPNPKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVFTQSKKL 65
           E++   K S+LNCIDL++ D++ SV  LKQACL+ GFFY +NHGI+EEFM++VF QSKKL
Sbjct: 2   ENHTTMKVSSLNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFEQSKKL 61

Query: 66  FSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDSDGEWP 125
           F+LPL +KMK+LRNEKHRGYTP+LDELLDP NQI GD+KEGYYIGIE  +P DD   + P
Sbjct: 62  FALPLEEKMKVLRNEKHRGYTPVLDELLDPKNQINGDHKEGYYIGIE--VPKDDPHWDKP 121

Query: 126 FYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEMLGEPIA 185
           FYGPN WP+ D+LPGWRE MEK+H++AL                       +EMLG+PIA
Sbjct: 122 FYGPNPWPDADVLPGWRETMEKYHQEAL-----------------------TEMLGKPIA 181

Query: 186 ILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWEYVAPL 245
            +RLL Y+G IS+P+KGI+  GAHSD+G++TLLATD  +GLQICKDK+A PQ WEYV P+
Sbjct: 182 TMRLLRYQG-ISDPSKGIYACGAHSDFGMMTLLATDGVMGLQICKDKNAMPQKWEYVPPI 241

Query: 246 KGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTCKSDKN 305
           KGAF+VNLGDMLERWSN  FKSTLHRVL NGQERYSI +FVEP+HDCL+ECLPTCKS+  
Sbjct: 242 KGAFIVNLGDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHDCLVECLPTCKSESE 301

Query: 306 PPKFPPILCKTYLSQRYNETHVDLNTY 333
            PK+PPI C TYL+QRY ETH +L+ Y
Sbjct: 302 LPKYPPIKCSTYLTQRYEETHANLSIY 302

BLAST of Tan0015425 vs. TAIR 10
Match: AT3G46490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 439.1 bits (1128), Expect = 3.2e-123
Identity = 203/330 (61.52%), Postives = 256/330 (77.58%), Query Frame = 0

Query: 1   MENPAESNPN-PKFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVF 60
           MEN  E   +  K S+L CIDL +SD+HQS  LLKQACL++GFFY +NHGI+EE  +E F
Sbjct: 1   MENKTEEEVSIIKVSSLTCIDLDNSDLHQSAVLLKQACLDSGFFYVINHGISEELKDEAF 60

Query: 61  TQSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDD 120
             SKK F+LPL +KMK+LRNEK+RGY P  D LLDP+NQ+RGDYKEG+ IG E    G  
Sbjct: 61  EHSKKFFALPLEEKMKVLRNEKYRGYAPFHDSLLDPENQVRGDYKEGFTIGFEGSKDGPH 120

Query: 121 SDGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEM 180
            D   PF+ PNIWP  D+LPGWRE MEK++++AL V K+IA+++A+AL L+ D+F+  EM
Sbjct: 121 WDK--PFHSPNIWPNPDVLPGWRETMEKYYQEALRVCKSIAKIMALALDLDVDYFNTPEM 180

Query: 181 LGEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNW 240
           LG PIA + L HYEG+ S+P+KGI+  GAHSD+G+++LLATD  +GLQICKDKD KPQ W
Sbjct: 181 LGNPIADMVLFHYEGK-SDPSKGIYACGAHSDFGMMSLLATDGVMGLQICKDKDVKPQKW 240

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPT 300
           EY   +KGA++VNLGD+LERWSN  FKSTLHRVL NGQ+RYSI +F++PSHDC+IECLPT
Sbjct: 241 EYTPSIKGAYIVNLGDLLERWSNGYFKSTLHRVLGNGQDRYSIPFFLKPSHDCIIECLPT 300

Query: 301 CKSDKNPPKFPPILCKTYLSQRYNETHVDL 330
           C+S+ N PK+P I C TY+SQRY   H  L
Sbjct: 301 CQSENNLPKYPAIKCSTYISQRYKAVHAHL 327

BLAST of Tan0015425 vs. TAIR 10
Match: AT3G46480.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 381.7 bits (979), Expect = 6.1e-106
Identity = 186/335 (55.52%), Postives = 237/335 (70.75%), Query Frame = 0

Query: 1   MENPAESNPNP-KFSALNCIDLSSSDIHQSVSLLKQACLETGFFYAVNHGITEEFMNEVF 60
           MEN  +   +  K S+L CIDL++ +  QS   LK                         
Sbjct: 1   MENKTQEEASTIKVSSLTCIDLANPNFQQSAVSLK------------------------- 60

Query: 61  TQSKKLFSLPLSDKMKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDD 120
            QSKK F+LPL +KMK+LRNEKHRGY+P+LD++LDP+NQ+ GDYKE ++IGIE       
Sbjct: 61  -QSKKFFALPLEEKMKVLRNEKHRGYSPVLDQILDPENQVDGDYKESFFIGIEV------ 120

Query: 121 SDGEWPFYGPNIWPEEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEM 180
                            +LPGWR  MEK+H++AL V KAIARL+A+AL L+ ++FDK EM
Sbjct: 121 -----------------VLPGWRATMEKYHQEALRVCKAIARLLALALDLDTNYFDKPEM 180

Query: 181 LGEPIAILRLLHYEGQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNW 240
           LG PIA++RLL YEG +S+P KGIFG GAHSDYG++TLLATD   GLQICKDKD KP+ W
Sbjct: 181 LGNPIAVMRLLRYEG-MSDPLKGIFGCGAHSDYGMLTLLATDSVTGLQICKDKDVKPRKW 240

Query: 241 EYVAPLKGAFVVNLGDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPT 300
           EYV  +KGA++VNLGD+LERWSN IFKSTLHRVL NGQ+RYSI +F+EPSHDCL+ECLPT
Sbjct: 241 EYVPSIKGAYIVNLGDLLERWSNGIFKSTLHRVLGNGQDRYSIPFFIEPSHDCLVECLPT 285

Query: 301 CKSDKNPPKFPPILCKTYLSQRYNETHVDLNTYKK 335
           C+S+ N PK+P I C T+L+QRY ++HV+L+ YK+
Sbjct: 301 CQSENNLPKYPAIKCSTFLTQRYQQSHVNLSIYKQ 285

BLAST of Tan0015425 vs. TAIR 10
Match: AT3G46500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 355.9 bits (912), Expect = 3.6e-98
Identity = 156/261 (59.77%), Postives = 208/261 (79.69%), Query Frame = 0

Query: 74  MKLLRNEKHRGYTPMLDELLDPDNQIRGDYKEGYYIGIEEKIPGDDSDGEWPFYGPNIWP 133
           MK+L+NEKH+GY+P+L ++   DNQI GDYKE ++IGIE          + PF   NIWP
Sbjct: 1   MKVLKNEKHQGYSPVLSQI--SDNQIHGDYKESFFIGIE-------GSNDTPFCRANIWP 60

Query: 134 EEDLLPGWREIMEKFHRQALEVAKAIARLIAVALGLENDFFDKSEMLGEPIAILRLLHYE 193
             D+L GW+  MEK+H++AL V KAIAR++A+AL ++ D+FD  EMLG P+  +RLLHYE
Sbjct: 61  NPDVLSGWQATMEKYHQEALRVCKAIARVLALALNVDGDYFDTPEMLGNPLTFMRLLHYE 120

Query: 194 GQISEPAKGIFGAGAHSDYGLITLLATDENLGLQICKDKDAKPQNWEYVAPLKGAFVVNL 253
           G +S+P+KGI+G G HSD+G++TLL TD  +GLQICKD+D KP+ WEY+  +KGA++VN+
Sbjct: 121 G-MSDPSKGIYGCGPHSDFGMMTLLGTDSVMGLQICKDRDVKPRKWEYILSIKGAYIVNI 180

Query: 254 GDMLERWSNCIFKSTLHRVLINGQERYSIAYFVEPSHDCLIECLPTCKSDKNPPKFPPIL 313
           GD+LERWSN IFKSTLHRVL NGQ+RYSIA+F++PSHDC++ECLPTC+S+ NPPK+P I 
Sbjct: 181 GDLLERWSNGIFKSTLHRVLGNGQDRYSIAFFLQPSHDCIVECLPTCQSENNPPKYPAIK 240

Query: 314 CKTYLSQRYNETHVDLNTYKK 335
           C TYL+QRY ++ VDL+ YKK
Sbjct: 241 CSTYLTQRYQDSQVDLSIYKK 251