Tan0014742 (gene) Snake gourd v1

Overview


Name	Tan0014742
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	calmodulin-like
Location	LG02: 85890018 .. 85890723 (+)
RNA-Seq Expression	Tan0014742
Synteny	Tan0014742

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAAGACATGTTTTTGAGGGGGAATAATTCGGTGTTGTCATAAAGAAAGAACATATAATTATGGGGCCATCATGAATTTGTACGTGAGAATAAATCATGTTCATAGGTCCCATACTATAAATACTCCATCAAAACACTCACCACTTCCGTCACAGCTCAAACTCACAAGCCAATATCCATACGTAGCAAACTAAACTAATTAAGCTCTCTTTCTCACAAAAATGGCTTGTCGTCCTGCAAGCAAGCTACAGCTCTGCCGGGAAGACATGAAGGAAATCTTCCGGGAGCACGACCTCGACGGAGATGGTGCCCTAAGCATGAGCGAGCTCATCAAATGCTTCGGGTTTATCGGTCATATTATGCCATTTTACAAAGCTCACTATGGCATGGCTTATGCTGATGAGGACGGTGATGGTCTCATTTCAGAGGACGAGCTCGACAAACTCATCGACTATGCCGAAAAGTTTGAACGCAAGAAGCATTAAGAAAATTGGCAACAACCCTATGTTGTTGTTGTACTACTCTATCGTTTTATGCTTTTCAATTTCAGTAATAAAGAAAAGAAGGGCTGGCTTGGTAGCTAGTTAGTCTTCTTTTGTTTATGTCATCTTGCTGGGAAATGGAAAAAGACTTCTAATATATATTTATTAGTTAATATGTGAGGTTAATCTGATTTGGACAATCATATATATAGACGTGCAAAGTT

mRNA sequence

ACAAGACATGTTTTTGAGGGGGAATAATTCGGTGTTGTCATAAAGAAAGAACATATAATTATGGGGCCATCATGAATTTGTACGTGAGAATAAATCATGTTCATAGGTCCCATACTATAAATACTCCATCAAAACACTCACCACTTCCGTCACAGCTCAAACTCACAAGCCAATATCCATACGTAGCAAACTAAACTAATTAAGCTCTCTTTCTCACAAAAATGGCTTGTCGTCCTGCAAGCAAGCTACAGCTCTGCCGGGAAGACATGAAGGAAATCTTCCGGGAGCACGACCTCGACGGAGATGGTGCCCTAAGCATGAGCGAGCTCATCAAATGCTTCGGGTTTATCGGTCATATTATGCCATTTTACAAAGCTCACTATGGCATGGCTTATGCTGATGAGGACGGTGATGGTCTCATTTCAGAGGACGAGCTCGACAAACTCATCGACTATGCCGAAAAGTTTGAACGCAAGAAGCATTAAGAAAATTGGCAACAACCCTATGTTGTTGTTGTACTACTCTATCGTTTTATGCTTTTCAATTTCAGTAATAAAGAAAAGAAGGGCTGGCTTGGTAGCTAGTTAGTCTTCTTTTGTTTATGTCATCTTGCTGGGAAATGGAAAAAGACTTCTAATATATATTTATTAGTTAATATGTGAGGTTAATCTGATTTGGACAATCATATATATAGACGTGCAAAGTT

Coding sequence (CDS)

ATGGCTTGTCGTCCTGCAAGCAAGCTACAGCTCTGCCGGGAAGACATGAAGGAAATCTTCCGGGAGCACGACCTCGACGGAGATGGTGCCCTAAGCATGAGCGAGCTCATCAAATGCTTCGGGTTTATCGGTCATATTATGCCATTTTACAAAGCTCACTATGGCATGGCTTATGCTGATGAGGACGGTGATGGTCTCATTTCAGAGGACGAGCTCGACAAACTCATCGACTATGCCGAAAAGTTTGAACGCAAGAAGCATTAA

Protein sequence

MACRPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDGDGLISEDELDKLIDYAEKFERKKH

Homology

[Close]

BLAST of Tan0014742 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 4.0e-06
Identity = 28/62 (45.16%), Postives = 36/62 (58.06%), Query Frame = 0


Query: 14  EDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDGDGLISEDELD 73
           E+M+ +FR+ D +GDG +S SEL   F  +GH     +    MA AD DGDG IS DE  
Sbjct: 39  EEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFA 98

Query: 74  KL 76
            L
Sbjct: 99  AL 100

[Close]

BLAST of Tan0014742 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 2.0e-05
Identity = 26/68 (38.24%), Postives = 38/68 (55.88%), Query Frame = 0


Query: 14 EDMKEIFREHDLDGDGALSMSELIKCF-GFIGHIMPFYKAHYGMAYADEDGDGLISEDEL 73
          ++ + +F   D DGDG +S +EL  C    +G  MP  +    MA AD DGDGL+ E+E 
Sbjct: 5  DEFRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEF 64

Query: 74 DKLIDYAE 81
           +L+   E
Sbjct: 65 VRLVTEME 72

[Close]

BLAST of Tan0014742 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 4.4e-05
Identity = 26/74 (35.14%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 3   CRPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADED 62
           CRP+  + + + ++ E FR  D DG+G +S +EL +    +GH + + +    M  AD D
Sbjct: 165 CRPS--VAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGHPICYAELTDMMREADTD 224

Query: 63  GDGLISEDELDKLI 77
           GDGLIS +E   ++
Sbjct: 225 GDGLISFEEFTAIM 236

[Close]

BLAST of Tan0014742 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 1.7e-04
Identity = 21/64 (32.81%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 13  REDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDGDGLISEDEL 72
           +E + E+FR  D DG+G+++ +EL      +GH + + +    M  AD +GDG+IS +E 
Sbjct: 85  QEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEADSNGDGVISFNEF 144

Query: 73  DKLI 77
             ++
Sbjct: 145 SHIM 148

[Close]

BLAST of Tan0014742 vs. NCBI nr
Match: KAA0060892.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 138.3 bits (347), Expect = 3.2e-29
Identity = 62/83 (74.70%), Postives = 74/83 (89.16%), Query Frame = 0


Query: 4  RPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDG 63
          R ASKLQL RED+KEIFREHD+DGDG LS+SE++K FGF G I+PFYKAHYGMA+ADE+G
Sbjct: 3  RAASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADENG 62

Query: 64 DGLISEDELDKLIDYAEKFERKK 87
          DG I E+ELDKL+DYAEKF+ K+
Sbjct: 63 DGYIEEEELDKLVDYAEKFQHKR 85

[Close]

BLAST of Tan0014742 vs. NCBI nr
Match: KGN62525.1 (hypothetical protein Csa_022453 [Cucumis sativus])

HSP 1 Score: 135.6 bits (340), Expect = 2.1e-28
Identity = 62/86 (72.09%), Postives = 73/86 (84.88%), Query Frame = 0


Query: 1  MACRPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYAD 60
          MA    SK    RE+MKEIFREHD+DGDG LS+SELIK  GF+GH +PFYKAHYGMAY D
Sbjct: 1  MALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCD 60

Query: 61 EDGDGLISEDELDKLIDYAEKFERKK 87
          E+GDGLISEDELDKLIDYAE+F++++
Sbjct: 61 ENGDGLISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of Tan0014742 vs. NCBI nr
Match: KGN62516.1 (hypothetical protein Csa_022521 [Cucumis sativus])

HSP 1 Score: 132.1 bits (331), Expect = 2.3e-27
Identity = 58/83 (69.88%), Postives = 73/83 (87.95%), Query Frame = 0


Query: 4  RPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDG 63
          R A++LQL RED+KEIFREHD+DGDG LSM+E++K FG  G I+PFYKAHYGMA+ADE+G
Sbjct: 3  RAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENG 62

Query: 64 DGLISEDELDKLIDYAEKFERKK 87
          DG I E+ELDKL+DYA+KF+ K+
Sbjct: 63 DGFIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of Tan0014742 vs. NCBI nr
Match: KAA0060886.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 130.6 bits (327), Expect = 6.7e-27
Identity = 61/83 (73.49%), Postives = 69/83 (83.13%), Query Frame = 0


Query: 1  MACRPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYAD 60
          MA    SK    RE+MKEIFREHD+DGDG LS+ ELIK  GF+GH +PFYKAHYGMAY+D
Sbjct: 1  MAHHGVSKFNFSREEMKEIFREHDIDGDGYLSIGELIKAMGFLGHSIPFYKAHYGMAYSD 60

Query: 61 EDGDGLISEDELDKLIDYAEKFE 84
          E+GDGLISEDELDKLIDYAE+ E
Sbjct: 61 ENGDGLISEDELDKLIDYAERKE 83

[Close]

BLAST of Tan0014742 vs. NCBI nr
Match: XP_004152805.1 (probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 129.4 bits (324), Expect = 1.5e-26
Identity = 56/81 (69.14%), Postives = 72/81 (88.89%), Query Frame = 0


Query: 6   ASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDGDG 65
           +++LQL RED+KEIFREHD+DGDG LSM+E++K FG  G I+PFYKAHYGMA+ADE+GDG
Sbjct: 86  STRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 145

Query: 66  LISEDELDKLIDYAEKFERKK 87
            I E+ELDKL+DYA+KF+ K+
Sbjct: 146 FIEEEELDKLVDYAQKFQHKR 166

[Close]

BLAST of Tan0014742 vs. ExPASy TrEMBL
Match: A0A5A7UYC0 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00250 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.6e-29
Identity = 62/83 (74.70%), Postives = 74/83 (89.16%), Query Frame = 0


Query: 4  RPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDG 63
          R ASKLQL RED+KEIFREHD+DGDG LS+SE++K FGF G I+PFYKAHYGMA+ADE+G
Sbjct: 3  RAASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADENG 62

Query: 64 DGLISEDELDKLIDYAEKFERKK 87
          DG I E+ELDKL+DYAEKF+ K+
Sbjct: 63 DGYIEEEELDKLVDYAEKFQHKR 85

[Close]

BLAST of Tan0014742 vs. ExPASy TrEMBL
Match: A0A0A0LKX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.0e-28
Identity = 62/86 (72.09%), Postives = 73/86 (84.88%), Query Frame = 0


Query: 1  MACRPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYAD 60
          MA    SK    RE+MKEIFREHD+DGDG LS+SELIK  GF+GH +PFYKAHYGMAY D
Sbjct: 1  MALPGVSKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCD 60

Query: 61 EDGDGLISEDELDKLIDYAEKFERKK 87
          E+GDGLISEDELDKLIDYAE+F++++
Sbjct: 61 ENGDGLISEDELDKLIDYAERFQKRR 86

[Close]

BLAST of Tan0014742 vs. ExPASy TrEMBL
Match: A0A0A0LRC1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.1e-27
Identity = 58/83 (69.88%), Postives = 73/83 (87.95%), Query Frame = 0


Query: 4  RPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDG 63
          R A++LQL RED+KEIFREHD+DGDG LSM+E++K FG  G I+PFYKAHYGMA+ADE+G
Sbjct: 3  RAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENG 62

Query: 64 DGLISEDELDKLIDYAEKFERKK 87
          DG I E+ELDKL+DYA+KF+ K+
Sbjct: 63 DGFIEEEELDKLVDYAQKFQHKR 85

[Close]

BLAST of Tan0014742 vs. ExPASy TrEMBL
Match: A0A5A7V2Z9 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00190 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 3.2e-27
Identity = 61/83 (73.49%), Postives = 69/83 (83.13%), Query Frame = 0


Query: 1  MACRPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYAD 60
          MA    SK    RE+MKEIFREHD+DGDG LS+ ELIK  GF+GH +PFYKAHYGMAY+D
Sbjct: 1  MAHHGVSKFNFSREEMKEIFREHDIDGDGYLSIGELIKAMGFLGHSIPFYKAHYGMAYSD 60

Query: 61 EDGDGLISEDELDKLIDYAEKFE 84
          E+GDGLISEDELDKLIDYAE+ E
Sbjct: 61 ENGDGLISEDELDKLIDYAERKE 83

[Close]

BLAST of Tan0014742 vs. ExPASy TrEMBL
Match: A0A6J1CSH1 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111013915 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.7e-24
Identity = 53/83 (63.86%), Postives = 68/83 (81.93%), Query Frame = 0


Query: 4   RPASKLQLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDG 63
           + A K++  R  +KEIF EHD+DGDG LS+SELIK F F+G ++P YKAHYG+A+AD+DG
Sbjct: 66  KKAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDG 125

Query: 64  DGLISEDELDKLIDYAEKFERKK 87
           DGLISE+ELDKL+DYAEK   +K
Sbjct: 126 DGLISEEELDKLVDYAEKGSDRK 148

[Close]

BLAST of Tan0014742 vs. TAIR 10
Match: AT3G25600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 46.6 bits (109), Expect = 1.2e-05
Identity = 21/64 (32.81%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 13  REDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDGDGLISEDEL 72
           +E + E+FR  D DG+G+++ +EL      +GH + + +    M  AD +GDG+IS +E 
Sbjct: 85  QEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEMMTEADSNGDGVISFNEF 144

Query: 73  DKLI 77
             ++
Sbjct: 145 SHIM 148

[Close]

BLAST of Tan0014742 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 42.0 bits (97), Expect = 2.9e-04
Identity = 19/62 (30.65%), Postives = 32/62 (51.61%), Query Frame = 0


Query: 10  QLCREDMKEIFREHDLDGDGALSMSELIKCFGFIGHIMPFYKAHYGMAYADEDGDGLISE 69
           Q  +E+++++F   D DGDG +S  EL   FG +G  +    A   +   D D DG +  
Sbjct: 59  QASKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGF 118

Query: 70  DE 72
           ++
Sbjct: 119 ED 120

The following BLAST results are available for this feature:

BLAST of Tan0014742 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RZB5	4.0e-06	45.16	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RYJ9	2.0e-05	38.24	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5ZD81	4.4e-05	35.14	Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9LI84	1.7e-04	32.81	Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ...	[more]

BLAST of Tan0014742 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0060892.1	3.2e-29	74.70	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
KGN62525.1	2.1e-28	72.09	hypothetical protein Csa_022453 [Cucumis sativus]	[more]
KGN62516.1	2.3e-27	69.88	hypothetical protein Csa_022521 [Cucumis sativus]	[more]
KAA0060886.1	6.7e-27	73.49	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
XP_004152805.1	1.5e-26	69.14	probable calcium-binding protein CML10 [Cucumis sativus]	[more]

BLAST of Tan0014742 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UYC0	1.6e-29	74.70	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LKX1	1.0e-28	72.09	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0LRC1	1.1e-27	69.88	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1	[more]
A0A5A7V2Z9	3.2e-27	73.49	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1CSH1	5.7e-24	63.86	probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Tan0014742 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25600.1	1.2e-05	32.81	Calcium-binding EF-hand family protein	[more]
AT3G50770.1	2.9e-04	30.65	calmodulin-like 41	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 15..41 e-value: 6.4E-7 score: 28.7
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 59..75 e-value: 0.0067 score: 15.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 11..46 score: 12.700205
None	No IPR available	GENE3D	1.10.238.10		coord: 3..82 e-value: 1.8E-11 score: 46.3
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 24..36
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 60..72
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0014742.1	Tan0014742.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding