Tan0013948 (gene) Snake gourd v1

Overview
NameTan0013948
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationLG04: 885074 .. 888888 (-)
RNA-Seq ExpressionTan0013948
SyntenyTan0013948
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCAAGTCCGCCAATAACCAATAGGCCTGTAGCTTTCACAAGCCTCGTCATTACTTCCAGTTCCCCCAAAGCTCAAAAAACGGCTTCCCCCATTTCCCAATTACAAAATAATCCTACGTTTAAGAGTGGTTGAATTGAAAATGATAGCAAAATTTAGATAGGAACAAACAAACCCAAATTAAAAAAAAAAAGTAAAAACCAAGAGAAGATCGATGAGGAGGTTTGTTTCGTTCTGTTTTATCGCTGCCGTGTGGCTGCAGGCGGGAGTGGGGCTGCCACTGCACACGGATTCACGGTGGATCGTGGACGAAGAAGGCCAAAGAGTGAAACTAAGATGCGTAAATTGGGTGGCGCATTTGGAGGCAGTGGTGGCGGAGGGGCTTTCAAAGCAGCCAATCGACGAGATTTCGAACCGAATTGTCTCACTAGGATTCAACTGCGTGAGGCTAACTTGGCCGCTCTTCTTAGCCACAAACGACTCTTTTAATTCCCTCACCGTTCGTCAGTCTTTCCAGCGGCTAGGCCTGGCCGAAGCCATCGCCGGAATCCAAGCCAATAACCCCTTCATCATCGATCTTCCTGTCATACAGGCTTTTGAGGTAAATGGGGGGTTTTTGAATTTTGCGAATCGTTGATGTGGGTTTGATTTTGATTTTGATTTAGATTTAGATTTTGAAACTCAGGCGGTGGTGGGGCGGCTGGGGGAGGCGAAGTTGATGGTGATATTGGACAATCACATAAGCAAGCCAGGGTGGTGCTGCAGCAGCTTCGACGGAAATGGGTTCTTTGGGGATCAGTATTTCAACCCGGAGACATGGATTAAGGGGCTCAATCGAATGGCCACCATGTTCAATGGTGTGGCCCATGTTGTTGCTATGAGCCTTAGAAACGAGCTTCGTGGCCCAAGACAGAATGTCAACGATTGGTACAGGTAAAAATTAAGGTCCACGACCTCTTAGCTTTATCTTTCCTTGATATGAATGAAATGGATGTTGATGAAGCAATACAATAATGGAAGGTACATGCAAAGAGGAGCAGAGACAGTTCATTCAGCAAATTCAGACATTCTTGTAATTCTCTCAGGACTGAGCTTCGACAAGGACTTATCATATCTGAAAACCCAACCCATAAACCTGACATTCACAGGGAAATTAGTGTACGAGGTTCATTGGTATGCGTTTTCAGATGGGTCCTCATGGGAATCAGGGAACTCAAACCAAGTGTGTGGAAGAGTGGTGAATAACATAATGAAAATGTCTGGTTTTGTATTGGAACAGGGGATGCCTCTGTTTGTGAGTGAATTTGGAGTGGATCAAAGAGGAAGCAATGTGAATGATAACAGATATTTGAGTTGCTTTTTGGCTGTGGCGGCTGAGTTTGATCTTGATTGGGCGATTTGGACACTTGTTGGAAGCTATTATTTGAGAGAAGGGGTTGTGGGTTTGAATGAGTTTTATGGATTGCTTGATTGGAATTGGTGTGCTCTTAGAAATTCTACCTTCCTTCAAAGGATTTCTGCCCTTCAATCTCCCTTTCAAGGTATCATTTCCTTTCATACTCTTCTTACACTTCCAATATTGAATGTGTTTTGGAGTAATTTAATTTTATTGCTTTTAACTCCTTAGGCTAAATTATACGAAATGCGATTAATTTCACTTTTATTCTCTAACGTTTTGAAATTTTCAATTTTACTTTTAATGTTTTTATTTCTTTCATAAAACTTCCTTGTTTAACTCCATTAAACGGCTCAATGCAAAATAAACTATTGAAGACAAAATTGAAACTTTGAAATCGTTAGAAGTAAATAAGGATGTCCATTTACCCTACACGGATGGAGCCTCGTGGGGTCTTGAGGAATAGAGGAGGTGGAAAAAAAAAAAAAAAAATACCGCGAGCTAAATGGAGATTGGATTTCCATCCTTGCCCTTGCCTCATTCCTGGTCCTCACCCATTTGCAATTTCTAATTTTTTATTATTATTATTATATAATATTTAAATTTAAAGTTTTATTATTTAATGACTTAAATAAATAATTTGATATATGTTGGAACGATTTGCTTGAACATTCGGGCTATATATAGATAATTATATAGTTTAATAGGTAATACTATTTTATTTCTATTGGGATGTTTAATAAATTTTTCTTTAAATGTAGATTTTAGGTTGGAATTAACCATTTAAATAAAAAAATTTCAAAAGAAAATTAAATATTAATAAAAAAATCAAAAGAATCCGTTGGGTTTTCTAAAAAACTAAATAGAGAATAATTCCATGAGAAAAATCCGATCCCTATGAAATTTAGCAGGAATAGAAAATTAAATGGGGGAGGGGAGGAGATGGGCATCTCTAGAACCAAAATTGAAACCAACCAATACCATACATTTAGAGAGAAAAATTAAAATCATCTACATTCATTAAGAGCATTTTGTATGATTTATTCGGTTGCAATTTTGTCCTAATTGATCTACATTTGTTTCTATTTACCTCTAACATTTTAAAATTTAATTATTATAAAAAATTTAATGTAGGTATTTTTGGTAACAAATTAAAAGATTGAGTGCAAAGTTGAAACTTTTAAAGCATTAAGGATCATTTAAACCAAAGTTCAAGGCCATTTTGGCTAGGTATTTCTTCTTTAAAAAAAAAGAGAAGTCATTTTAGCTAGTTTAGCATACCTGAATCTTTTCAGGGTCGTGTCCAATCTAGTTCTTGTAGTTTAAAAAGTTTAGATCACAAAAACTACATTACATAAGAATTTTGTGGTGTATCATGAGAAATCAAACTTACATAGATAATGAAAGAGACCTATTAGACACAAAATAGACGACATAGATACAATCTTAAAAATTCCTCGACATATTAGAATTAATAACGTACTCCAACCAACAAAAGGATAGCTCAAATGACATCAAGTATGACTTATGACCTCATGACCAAAAGGTCATGGGTTTGAATCCCCCACTCTAATTGTTGATGTACTAAAAAAAAAAAATACTCCGAGCTCAGCATTGGGGATGTGGTGATGTGAATTGCAGGGCCAGGACTAGCAGAAAGAAGGCAGTACAATGTAATCTTCCACCCATCAACAGGCCTATGCGTGGTGCGAAAATCACTAATAGATCCATTAAAATTAGGCCCATGCACTGACTCAGATCCTTGGTACTACACGCCACAGAAGTTTTTAACTCTCAAAGGTACATATTTTTGCATACAAGCAGACGAAATGGGGAAGCAAGCAAAATTGGGAATAATATGCACTGTTTCTAATGCAAAATGGGACATGATTTCTGATTCAAAGATGCATCTTTCTTCTAAAGCTACCGATGGCTCGACCGTTTGCTTGGACGTCGACCCGAGCACCAATGAACTTGTGACCAATGCTTGTAAATGCTTGACTCGGGACTCGACGTGCGACCCGAGTAGCCAATGGTTCAAGCTCGTTAATAGCACGAGAAGTTTGGGCGTGACGAGGTCGATGATTGATATGGTCGGTTCCTCGTTGGCTAATATTGTGCCCAAGTTTATGGAGTTGGGTTATGGCAGTATTTGAAGTGTAGAGGGAAAATAGTAGCTGGGGATGTGTTTAATTTATTACATAAGTCAAATTATAAACAGAGGACGTTTAATTTTAATTGGTTCTGTTTATTTTAGAAAATAAAAATAGATAAAAGTGGAAAAAAATTGCCCCCATGCAGGATCGAACTACAGACCTTCAGTTTACAAGACTGACGCTCTACCACTGAGCTATAGGGGCGATTGATAATTTTAAGTCATTTATACTTTTTCAATTAATAATATCTTTCCTCTTTATTAA

mRNA sequence

TTCCAAGTCCGCCAATAACCAATAGGCCTGTAGCTTTCACAAGCCTCGTCATTACTTCCAGTTCCCCCAAAGCTCAAAAAACGGCTTCCCCCATTTCCCAATTACAAAATAATCCTACGTTTAAGAGTGGTTGAATTGAAAATGATAGCAAAATTTAGATAGGAACAAACAAACCCAAATTAAAAAAAAAAAGTAAAAACCAAGAGAAGATCGATGAGGAGGTTTGTTTCGTTCTGTTTTATCGCTGCCGTGTGGCTGCAGGCGGGAGTGGGGCTGCCACTGCACACGGATTCACGGTGGATCGTGGACGAAGAAGGCCAAAGAGTGAAACTAAGATGCGTAAATTGGGTGGCGCATTTGGAGGCAGTGGTGGCGGAGGGGCTTTCAAAGCAGCCAATCGACGAGATTTCGAACCGAATTGTCTCACTAGGATTCAACTGCGTGAGGCTAACTTGGCCGCTCTTCTTAGCCACAAACGACTCTTTTAATTCCCTCACCGTTCGTCAGTCTTTCCAGCGGCTAGGCCTGGCCGAAGCCATCGCCGGAATCCAAGCCAATAACCCCTTCATCATCGATCTTCCTGTCATACAGGCTTTTGAGGCGGTGGTGGGGCGGCTGGGGGAGGCGAAGTTGATGGTGATATTGGACAATCACATAAGCAAGCCAGGGTGGTGCTGCAGCAGCTTCGACGGAAATGGGTTCTTTGGGGATCAGTATTTCAACCCGGAGACATGGATTAAGGGGCTCAATCGAATGGCCACCATGTTCAATGGTGTGGCCCATGTTGTTGCTATGAGCCTTAGAAACGAGCTTCGTGGCCCAAGACAGAATGTCAACGATTGGTACAGGTACATGCAAAGAGGAGCAGAGACAGTTCATTCAGCAAATTCAGACATTCTTGTAATTCTCTCAGGACTGAGCTTCGACAAGGACTTATCATATCTGAAAACCCAACCCATAAACCTGACATTCACAGGGAAATTAGTGTACGAGGTTCATTGGTATGCGTTTTCAGATGGGTCCTCATGGGAATCAGGGAACTCAAACCAAGTGTGTGGAAGAGTGGTGAATAACATAATGAAAATGTCTGGTTTTGTATTGGAACAGGGGATGCCTCTGTTTGTGAGTGAATTTGGAGTGGATCAAAGAGGAAGCAATGTGAATGATAACAGATATTTGAGTTGCTTTTTGGCTGTGGCGGCTGAGTTTGATCTTGATTGGGCGATTTGGACACTTGTTGGAAGCTATTATTTGAGAGAAGGGGTTGTGGGTTTGAATGAGTTTTATGGATTGCTTGATTGGAATTGGTGTGCTCTTAGAAATTCTACCTTCCTTCAAAGGATTTCTGCCCTTCAATCTCCCTTTCAAGGGCCAGGACTAGCAGAAAGAAGGCAGTACAATGTAATCTTCCACCCATCAACAGGCCTATGCGTGGTGCGAAAATCACTAATAGATCCATTAAAATTAGGCCCATGCACTGACTCAGATCCTTGGTACTACACGCCACAGAAGTTTTTAACTCTCAAAGGTACATATTTTTGCATACAAGCAGACGAAATGGGGAAGCAAGCAAAATTGGGAATAATATGCACTGTTTCTAATGCAAAATGGGACATGATTTCTGATTCAAAGATGCATCTTTCTTCTAAAGCTACCGATGGCTCGACCGTTTGCTTGGACGTCGACCCGAGCACCAATGAACTTGTGACCAATGCTTGTAAATGCTTGACTCGGGACTCGACGTGCGACCCGAGTAGCCAATGGTTCAAGCTCGTTAATAGCACGAGAAGTTTGGGCGTGACGAGGTCGATGATTGATATGGTCGGTTCCTCGTTGGCTAATATTGTGCCCAAGTTTATGGAGTTGGGTTATGGCAGTATTTGAAGTGTAGAGGGAAAATAGTAGCTGGGGATGTGTTTAATTTATTACATAAGTCAAATTATAAACAGAGGACGTTTAATTTTAATTGGTTCTGTTTATTTTAGAAAATAAAAATAGATAAAAGTGGAAAAAAATTGCCCCCATGCAGGATCGAACTACAGACCTTCAGTTTACAAGACTGACGCTCTACCACTGAGCTATAGGGGCGATTGATAATTTTAAGTCATTTATACTTTTTCAATTAATAATATCTTTCCTCTTTATTAA

Coding sequence (CDS)

ATGAGGAGGTTTGTTTCGTTCTGTTTTATCGCTGCCGTGTGGCTGCAGGCGGGAGTGGGGCTGCCACTGCACACGGATTCACGGTGGATCGTGGACGAAGAAGGCCAAAGAGTGAAACTAAGATGCGTAAATTGGGTGGCGCATTTGGAGGCAGTGGTGGCGGAGGGGCTTTCAAAGCAGCCAATCGACGAGATTTCGAACCGAATTGTCTCACTAGGATTCAACTGCGTGAGGCTAACTTGGCCGCTCTTCTTAGCCACAAACGACTCTTTTAATTCCCTCACCGTTCGTCAGTCTTTCCAGCGGCTAGGCCTGGCCGAAGCCATCGCCGGAATCCAAGCCAATAACCCCTTCATCATCGATCTTCCTGTCATACAGGCTTTTGAGGCGGTGGTGGGGCGGCTGGGGGAGGCGAAGTTGATGGTGATATTGGACAATCACATAAGCAAGCCAGGGTGGTGCTGCAGCAGCTTCGACGGAAATGGGTTCTTTGGGGATCAGTATTTCAACCCGGAGACATGGATTAAGGGGCTCAATCGAATGGCCACCATGTTCAATGGTGTGGCCCATGTTGTTGCTATGAGCCTTAGAAACGAGCTTCGTGGCCCAAGACAGAATGTCAACGATTGGTACAGGTACATGCAAAGAGGAGCAGAGACAGTTCATTCAGCAAATTCAGACATTCTTGTAATTCTCTCAGGACTGAGCTTCGACAAGGACTTATCATATCTGAAAACCCAACCCATAAACCTGACATTCACAGGGAAATTAGTGTACGAGGTTCATTGGTATGCGTTTTCAGATGGGTCCTCATGGGAATCAGGGAACTCAAACCAAGTGTGTGGAAGAGTGGTGAATAACATAATGAAAATGTCTGGTTTTGTATTGGAACAGGGGATGCCTCTGTTTGTGAGTGAATTTGGAGTGGATCAAAGAGGAAGCAATGTGAATGATAACAGATATTTGAGTTGCTTTTTGGCTGTGGCGGCTGAGTTTGATCTTGATTGGGCGATTTGGACACTTGTTGGAAGCTATTATTTGAGAGAAGGGGTTGTGGGTTTGAATGAGTTTTATGGATTGCTTGATTGGAATTGGTGTGCTCTTAGAAATTCTACCTTCCTTCAAAGGATTTCTGCCCTTCAATCTCCCTTTCAAGGGCCAGGACTAGCAGAAAGAAGGCAGTACAATGTAATCTTCCACCCATCAACAGGCCTATGCGTGGTGCGAAAATCACTAATAGATCCATTAAAATTAGGCCCATGCACTGACTCAGATCCTTGGTACTACACGCCACAGAAGTTTTTAACTCTCAAAGGTACATATTTTTGCATACAAGCAGACGAAATGGGGAAGCAAGCAAAATTGGGAATAATATGCACTGTTTCTAATGCAAAATGGGACATGATTTCTGATTCAAAGATGCATCTTTCTTCTAAAGCTACCGATGGCTCGACCGTTTGCTTGGACGTCGACCCGAGCACCAATGAACTTGTGACCAATGCTTGTAAATGCTTGACTCGGGACTCGACGTGCGACCCGAGTAGCCAATGGTTCAAGCTCGTTAATAGCACGAGAAGTTTGGGCGTGACGAGGTCGATGATTGATATGGTCGGTTCCTCGTTGGCTAATATTGTGCCCAAGTTTATGGAGTTGGGTTATGGCAGTATTTGA

Protein sequence

MRRFVSFCFIAAVWLQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQPIDEISNRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNRMATMFNGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKDLSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGMPLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGLLDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGPCTDSDPWYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKATDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSSLANIVPKFMELGYGSI
Homology
BLAST of Tan0013948 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 4.3e-130
Identity = 245/526 (46.58%), Postives = 324/526 (61.60%), Query Frame = 0

Query: 21  LPLHTDSRWIVDE-EGQRVKLRCVNWVAHLEAVVAEGLSKQPIDEISNRIVSLGFNCVRL 80
           LPL T  RWIVDE  G RVKL CVNWV HLE  + EGL++ P+  +++ I SLGFNCVRL
Sbjct: 29  LPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVAHTISSLGFNCVRL 88

Query: 81  TWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPVIQAFEAVVGRLGEAK 140
           T+ + + T  S+ + TV Q+F RL L EA +GI+ NNP ++DL  + A+  VV  L EA 
Sbjct: 89  TYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVAAYHHVVAALSEAG 148

Query: 141 LMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNRMATMFNGVAHVVAMSLRNE 200
           +MVILDNH+SKP WCC+  DGNGFFGD+YFNP TW++GL  MAT FN   +VVAMSLRNE
Sbjct: 149 VMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFNNTPNVVAMSLRNE 208

Query: 201 LRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKDLSYLKTQPINLTFTGKLVY 260
           LRG R     W R+MQ GA TVH AN  +LVILSGL FD DLS+L   P+ L F  K+VY
Sbjct: 209 LRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPVLPVTLPFKEKIVY 268

Query: 261 EVHWYAFSDGSSWESGNSNQVC----GRVVNNIMKMSGFVLEQGMPLFVSEFGVDQRGSN 320
           E HWY+F  G  W +G  N VC    GR  +N+  ++        PLF+SEFG+DQR  N
Sbjct: 269 EGHWYSF--GVPWRTGLPNDVCKNETGRFKSNVGFVTSSANATAAPLFMSEFGIDQRYVN 328

Query: 321 VNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLR---EGVVGLNEFYGLLDWNWCALRNST 380
            NDNRYL+C LA  AE DLDWA+WT+ GSYY R   + V    E YG  + +W  +RN  
Sbjct: 329 DNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEETYGFFNHDWSRIRNPD 388

Query: 381 FLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGPCTD-SDPWYY-- 440
           F+ R+  +Q P Q P LA    Y +I+HP++GLC V   + + + LG C      W Y  
Sbjct: 389 FISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLC-VESGIGNTVHLGSCQSVRSRWNYDA 448

Query: 441 TPQKFLTLKGTYFCIQADEMGKQAKLGIICTV-SNAKWDMISDSKMHLSSK--ATDGSTV 500
           + +  + L G+  CI     G  A +   C+  +N  W  +S +++ L ++    DG   
Sbjct: 449 SVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQLQLGTRVLGKDGKEK 508

Query: 501 CLDVDPSTNELV-TNACKCLTRDSTC----DPSSQWFKLVNSTRSL 528
            + +D S + L+ TN C C+T DS C    +P  QWFK++ + + L
Sbjct: 509 WMCLDGSKSPLISTNECICIT-DSHCYPKLNPEKQWFKVITTNKQL 550

BLAST of Tan0013948 vs. NCBI nr
Match: XP_038893388.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.4e-303
Identity = 489/555 (88.11%), Postives = 522/555 (94.05%), Query Frame = 0

Query: 1   MRRFVSFCFIAAVWLQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQ 60
           M+  + FC +AAVWLQA VGLPLHTDSRWIVDEEGQRVKLRCVNWV+HLEAVVAEGLSKQ
Sbjct: 8   MKGLILFCSVAAVWLQAAVGLPLHTDSRWIVDEEGQRVKLRCVNWVSHLEAVVAEGLSKQ 67

Query: 61  PIDEISNRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIDEISNRI S GFNCVRLTWPLFLATNDS NSLTVRQSFQRLGLAEAIAGIQANNPFII
Sbjct: 68  PIDEISNRIESFGFNCVRLTWPLFLATNDSLNSLTVRQSFQRLGLAEAIAGIQANNPFII 127

Query: 121 DLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNR 180
           DLP+I+AFEAVVG+LGE KLMVILDNHISKPGWCCS+FDGNGFFGDQYF+PE WIKGL R
Sbjct: 128 DLPLIKAFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFSPELWIKGLTR 187

Query: 181 MATMFNGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKD 240
           MA MFNGVAHVV MSLRNELRGP+QNVNDWYRYMQRGAE VHSAN D+L+ILSGLSFD+D
Sbjct: 188 MAAMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDVLIILSGLSFDRD 247

Query: 241 LSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGM 300
           LS+LK QPINLTFT KLVYEVHWY FSDGSSWESGNSNQVCGRV NN+MKMSGF+LEQG 
Sbjct: 248 LSFLKNQPINLTFTAKLVYEVHWYGFSDGSSWESGNSNQVCGRVTNNLMKMSGFILEQGF 307

Query: 301 PLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGL 360
           PLF+SEFGVDQRG+N+NDNRYLSCFL+VAAEFDLDWA+WTLVGSYYLREGVVGLNEFYG+
Sbjct: 308 PLFISEFGVDQRGTNINDNRYLSCFLSVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGI 367

Query: 361 LDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGP 420
           LDWNWC+LRNS+FLQRISALQSPFQGPGL ERRQYNVIFHP TGLCVVRKSL+DPLKLGP
Sbjct: 368 LDWNWCSLRNSSFLQRISALQSPFQGPGLEERRQYNVIFHPLTGLCVVRKSLLDPLKLGP 427

Query: 421 CTDSDPWYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKA 480
           C DSDPWYYTPQKFLTLKGTYFCIQA+EMGKQAKLGIICTVSNAKWDMISDSKMHLSSK 
Sbjct: 428 CVDSDPWYYTPQKFLTLKGTYFCIQAEEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKV 487

Query: 481 TDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSS 540
           ++GS VCLDVD STNE+VTN+CKCL RDS CDPSSQWFKLVNSTRSLG  RSM+ MVGSS
Sbjct: 488 SNGSMVCLDVDSSTNEIVTNSCKCLGRDSACDPSSQWFKLVNSTRSLGRARSMVSMVGSS 547

Query: 541 LANIVPKFMELGYGS 556
           L N+V KF+ELGYGS
Sbjct: 548 LGNVVTKFVELGYGS 562

BLAST of Tan0013948 vs. NCBI nr
Match: XP_023514558.1 (uncharacterized protein LOC111778811 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1023.8 bits (2646), Expect = 5.3e-295
Identity = 476/548 (86.86%), Postives = 519/548 (94.71%), Query Frame = 0

Query: 1   MRRFVSFCFIAAVWLQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQ 60
           M+  V  C +AA+WLQA VGLPLHTDSRWIVDE+GQRVKLRCVNWV+HLEAVVAEGLSKQ
Sbjct: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEISNRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIDEI+NRI SLGFNCVRLTWPLFLATN+S +SLTVRQSFQRLGL EAIAG+QANNPFII
Sbjct: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 120

Query: 121 DLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNR 180
           DLP+I+AFEAVVGRLGEA+LMV+LDNHISKPGWCCS+FDGNGFFGDQYFNPE WI+GL R
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 MATMFNGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKD 240
           MATMFNGVAHVV MSLRNELRGP+QNVNDWYRYMQRGAE VH+AN D+LVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGM 300
           LS+LKTQPINLTFT KLVYEVHWYAFSDGSSW+SGN NQVCGRVVNN+MKMSGF+LEQGM
Sbjct: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300

Query: 301 PLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGL 360
           PLF++EFGVDQRG+NVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGP 420
           LDWNWC  RNS+FLQRISALQ+PFQGPGLAERR Y+V+FHPSTGLCV RKSL+DPL+LGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CTDSDPWYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKA 480
           C DSDPWYYTPQKFLTLKGTYFCIQA +MGKQ +LGIICTVSNA+WDM+SDSKMHLSSK 
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 TDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSS 540
            DGS VCLDVDPSTNE+VTNACKCL+RDS+CDPSSQWFKLVNSTRSLG TRSMI MVGSS
Sbjct: 481 DDGSVVCLDVDPSTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGATRSMISMVGSS 540

Query: 541 L-ANIVPK 548
           L +N+VPK
Sbjct: 541 LSSNLVPK 548

BLAST of Tan0013948 vs. NCBI nr
Match: KAG6601558.1 (hypothetical protein SDJN03_06791, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1020.4 bits (2637), Expect = 5.9e-294
Identity = 475/548 (86.68%), Postives = 518/548 (94.53%), Query Frame = 0

Query: 1   MRRFVSFCFIAAVWLQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQ 60
           MR  V  C +AA+WLQA VGLPLHTDSRWIVDE+GQRVKLRCVNWV+HLEAVVAEGLSKQ
Sbjct: 1   MRGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEISNRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIDEI+NRI SLGFNCVRLTWPLFLATN+S +SLTVRQSFQRLGL EAIAGIQANNPFII
Sbjct: 61  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNR 180
           DLP+I+AFEAVVGRLGEA+LMV+LDNHISKPGWCCS+FDGNGFFGDQYFNPE WI+GL R
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 MATMFNGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKD 240
           MATMFNGVAHVV MSLRNELRGP+QNVNDWYRYMQRGAE VH+AN D+LVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGM 300
           LS+LKTQPINLTFT KLVYEVHWYAFSDGSSW+SGN NQVCGRVVNN++KMSGF+LEQGM
Sbjct: 241 LSFLKTQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLLKMSGFLLEQGM 300

Query: 301 PLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGL 360
           PLF++EFGVDQRG+NVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGP 420
           LDWNWC  RNS+FLQRISALQ+PFQGPGLAERR Y+V+FHPSTGLCV RKSL+DPL+LGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CTDSDPWYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKA 480
           C DSDPWYYTPQKFLTLKGTYFCIQA +MGKQ +LGIICTVSNA+WDM+SDSKMHLSSK 
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 TDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSS 540
            DGS VCLDVDPS+NE+VTNACKCL+RDS+CDPSSQWFKLVNSTRSLG  RSMI MVGSS
Sbjct: 481 DDGSMVCLDVDPSSNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSLGAARSMISMVGSS 540

Query: 541 L-ANIVPK 548
           L +N+VPK
Sbjct: 541 LSSNLVPK 548

BLAST of Tan0013948 vs. NCBI nr
Match: KAG7032321.1 (hypothetical protein SDJN02_06366, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1018.1 bits (2631), Expect = 2.9e-293
Identity = 474/548 (86.50%), Postives = 518/548 (94.53%), Query Frame = 0

Query: 1   MRRFVSFCFIAAVWLQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQ 60
           MR  V  C +AA+WLQA VGLPLHTDSRWIVDE+GQRVKLRCVNWV+HLEAVVAEGLSKQ
Sbjct: 1   MRGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEISNRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIDEI+NRI  LGFNCVRLTWPLFLATN+S +SLTVRQSFQRLGL EAIAGIQANNPFII
Sbjct: 61  PIDEITNRIGLLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNR 180
           DLP+I+AFEAVVGRLGEA+LMV+LDNHISKPGWCCS+FDGNGFFGDQYFNPE WI+GL R
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 MATMFNGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKD 240
           MATMFNGVAHVV MSLRNELRGP+QNVNDWYRYMQRGAE VH+AN D+LVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGM 300
           LS+LKTQPINLTFT KLVYEVHWYAFSDGSSW+SGN NQVCGRVVNN++KMSGF+LEQGM
Sbjct: 241 LSFLKTQPINLTFTSKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLLKMSGFLLEQGM 300

Query: 301 PLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGL 360
           PLF++EFGVDQRG+NVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGP 420
           LDWNWC  RNS+FLQRISALQ+PFQGPGLAERR Y+V+FHPSTGLCV RKSL+DPL+LGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CTDSDPWYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKA 480
           C DSDPWYYTPQKFLTLKGTYFCIQA +MGKQ +LGIICTVSNA+WDM+SDS+MHLSSK 
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSRMHLSSKL 480

Query: 481 TDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSS 540
            DGS VCLDVDPSTNE+VTNACKCL++DS+CDPSSQWFKLVNSTRSLG TRSMI MVGSS
Sbjct: 481 EDGSVVCLDVDPSTNEIVTNACKCLSQDSSCDPSSQWFKLVNSTRSLGRTRSMISMVGSS 540

Query: 541 L-ANIVPK 548
           L +N+VPK
Sbjct: 541 LSSNLVPK 548

BLAST of Tan0013948 vs. NCBI nr
Match: XP_022957392.1 (uncharacterized protein LOC111458804 [Cucurbita moschata])

HSP 1 Score: 1016.5 bits (2627), Expect = 8.5e-293
Identity = 473/548 (86.31%), Postives = 517/548 (94.34%), Query Frame = 0

Query: 1   MRRFVSFCFIAAVWLQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQ 60
           M+  V  C +AA+WLQA VGLPLHTDSRWIVDE+GQRVKLRCVNWV+HLEAVVAEGLSKQ
Sbjct: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEISNRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIDEI+NRI  LGFNCVRLTWPLFLATN+S +SLTVRQSFQRLGL EAIAGIQANNPFII
Sbjct: 61  PIDEITNRIGLLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNR 180
           DLP+I+AFEAVVGRLGEA+LMV+LDNHISKPGWCCS+FDGNGFFGDQYFNPE WI+GL R
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 MATMFNGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKD 240
           MATMFNGVAHVV MSLRNELRGP+QNVNDWYRYMQRGAE VH+AN D+LVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGM 300
           LS+LKTQPINLTFT KLVYEVHWYAFSDGSSW+SGN NQVCGRVVNN+MKMSGF+LEQGM
Sbjct: 241 LSFLKTQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300

Query: 301 PLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGL 360
           PLF++EFGVDQRG+NVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGP 420
           LDWNWC  RNS+FLQRISALQ+PFQGPGLAERR Y+V+FHPSTGLCV RKSL+DPL+LGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CTDSDPWYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKA 480
           C DSDPWYYTPQKFLTLKGTYFCIQA +MGKQ +LGIICTVSNA+WDM+SDSKMHLSSK 
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 TDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSS 540
            DGS VCLDVDPS+NE+VTNACKCL+RDS+C+PSSQWFKLVNSTRSLG  RSMI MVGSS
Sbjct: 481 DDGSVVCLDVDPSSNEIVTNACKCLSRDSSCNPSSQWFKLVNSTRSLGAARSMISMVGSS 540

Query: 541 L-ANIVPK 548
           L +N+VPK
Sbjct: 541 LSSNLVPK 548

BLAST of Tan0013948 vs. ExPASy TrEMBL
Match: A0A6J1GZ00 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111458804 PE=3 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 4.1e-293
Identity = 473/548 (86.31%), Postives = 517/548 (94.34%), Query Frame = 0

Query: 1   MRRFVSFCFIAAVWLQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQ 60
           M+  V  C +AA+WLQA VGLPLHTDSRWIVDE+GQRVKLRCVNWV+HLEAVVAEGLSKQ
Sbjct: 1   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 60

Query: 61  PIDEISNRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIDEI+NRI  LGFNCVRLTWPLFLATN+S +SLTVRQSFQRLGL EAIAGIQANNPFII
Sbjct: 61  PIDEITNRIGLLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGIQANNPFII 120

Query: 121 DLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNR 180
           DLP+I+AFEAVVGRLGEA+LMV+LDNHISKPGWCCS+FDGNGFFGDQYFNPE WI+GL R
Sbjct: 121 DLPLIKAFEAVVGRLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 180

Query: 181 MATMFNGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKD 240
           MATMFNGVAHVV MSLRNELRGP+QNVNDWYRYMQRGAE VH+AN D+LVILSGLSFDKD
Sbjct: 181 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 240

Query: 241 LSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGM 300
           LS+LKTQPINLTFT KLVYEVHWYAFSDGSSW+SGN NQVCGRVVNN+MKMSGF+LEQGM
Sbjct: 241 LSFLKTQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 300

Query: 301 PLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGL 360
           PLF++EFGVDQRG+NVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYGL
Sbjct: 301 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 360

Query: 361 LDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGP 420
           LDWNWC  RNS+FLQRISALQ+PFQGPGLAERR Y+V+FHPSTGLCV RKSL+DPL+LGP
Sbjct: 361 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRKSLLDPLRLGP 420

Query: 421 CTDSDPWYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKA 480
           C DSDPWYYTPQKFLTLKGTYFCIQA +MGKQ +LGIICTVSNA+WDM+SDSKMHLSSK 
Sbjct: 421 CADSDPWYYTPQKFLTLKGTYFCIQAQDMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 480

Query: 481 TDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSS 540
            DGS VCLDVDPS+NE+VTNACKCL+RDS+C+PSSQWFKLVNSTRSLG  RSMI MVGSS
Sbjct: 481 DDGSVVCLDVDPSSNEIVTNACKCLSRDSSCNPSSQWFKLVNSTRSLGAARSMISMVGSS 540

Query: 541 L-ANIVPK 548
           L +N+VPK
Sbjct: 541 LSSNLVPK 548

BLAST of Tan0013948 vs. ExPASy TrEMBL
Match: A0A6J1KEP8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111492584 PE=3 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 3.0e-291
Identity = 470/548 (85.77%), Postives = 514/548 (93.80%), Query Frame = 0

Query: 1   MRRFVSFCFIAAVWLQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQ 60
           M+  V  C +AA+WLQA VGLPLHTDSRWIVDE+GQRVKLRCVNWV+HLEAVVAEGLSKQ
Sbjct: 2   MKGLVLLCSVAALWLQAAVGLPLHTDSRWIVDEQGQRVKLRCVNWVSHLEAVVAEGLSKQ 61

Query: 61  PIDEISNRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFII 120
           PIDEI+NRI SLGFNCVRLTWPLFLATN+S +SLTVRQSFQRLGL EAIAG+QANNPFII
Sbjct: 62  PIDEITNRIGSLGFNCVRLTWPLFLATNESLSSLTVRQSFQRLGLREAIAGVQANNPFII 121

Query: 121 DLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNR 180
           DLP+I+AFEAVVG LGEA+LMV+LDNHISKPGWCCS+FDGNGFFGDQYFNPE WI+GL R
Sbjct: 122 DLPLIKAFEAVVGWLGEAELMVVLDNHISKPGWCCSNFDGNGFFGDQYFNPEKWIEGLTR 181

Query: 181 MATMFNGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKD 240
           MATMFNGVAHVV MSLRNELRGP+QNVNDWYRYMQRGAE VH+AN D+LVILSGLSFDKD
Sbjct: 182 MATMFNGVAHVVGMSLRNELRGPKQNVNDWYRYMQRGAEAVHAANPDVLVILSGLSFDKD 241

Query: 241 LSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGM 300
           LS+LK QPINLTFT KLVYEVHWYAFSDGSSW+SGN NQVCGRVVNN+MKMSGF+LEQGM
Sbjct: 242 LSFLKNQPINLTFTAKLVYEVHWYAFSDGSSWQSGNPNQVCGRVVNNLMKMSGFLLEQGM 301

Query: 301 PLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGL 360
           PLF++EFGVDQRG+NVNDNRYLSCFL+VAAE+DLDWA+WTLVGSYYLREGVVGLNEFYGL
Sbjct: 302 PLFLTEFGVDQRGTNVNDNRYLSCFLSVAAEYDLDWAVWTLVGSYYLREGVVGLNEFYGL 361

Query: 361 LDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGP 420
           LDWNWC  RNS+FLQRISALQ+PFQGPGLAERR Y+V+FHPSTGLCV R SL+DPL+LGP
Sbjct: 362 LDWNWCNPRNSSFLQRISALQTPFQGPGLAERRPYSVMFHPSTGLCVGRASLLDPLRLGP 421

Query: 421 CTDSDPWYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKA 480
           C DSDPWYYTPQKFLTLKGTYFCIQA EMGKQ +LGIICTVSNA+WDM+SDSKMHLSSK 
Sbjct: 422 CADSDPWYYTPQKFLTLKGTYFCIQAQEMGKQPRLGIICTVSNAQWDMVSDSKMHLSSKL 481

Query: 481 TDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSS 540
            +GS VCLDVDP+TNE+VTNACKCL+RDS+CDPSSQWFKLVNSTRS   TRSMI MVGSS
Sbjct: 482 DNGSVVCLDVDPNTNEIVTNACKCLSRDSSCDPSSQWFKLVNSTRSFDTTRSMISMVGSS 541

Query: 541 LA-NIVPK 548
           L+ N+VPK
Sbjct: 542 LSFNLVPK 549

BLAST of Tan0013948 vs. ExPASy TrEMBL
Match: A0A5D3CXK4 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001710 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.4e-288
Identity = 465/545 (85.32%), Postives = 509/545 (93.39%), Query Frame = 0

Query: 10  IAAVW---LQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQPIDEIS 69
           + AVW     A VGLPLHTD+RWIVD EG+RVKLRCVNWV+HLEAVVAEGLSKQPI+EIS
Sbjct: 8   LLAVWCVAASAAVGLPLHTDTRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQPIEEIS 67

Query: 70  NRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPVIQ 129
           NRI  LGFNCVRLTWPLFLATN+S NSLTVRQSFQRLGL EAIAGIQANNPFIIDLP+++
Sbjct: 68  NRIEGLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLTEAIAGIQANNPFIIDLPLLK 127

Query: 130 AFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNRMATMFN 189
           AFEAVVG+LGE KLMVILDNHISKPGWCCS+FDGNGFFGDQYFNP+ WIKGL RMATMFN
Sbjct: 128 AFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMATMFN 187

Query: 190 GVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKDLSYLKT 249
           GV HVVAMSLRNELRGP+QNVNDWYRYMQRGAE VHSAN DIL+ILSGLSFD+DLS+LK 
Sbjct: 188 GVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLSFLKN 247

Query: 250 QPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGMPLFVSE 309
           QPINLTFT K VYEVHWYAFSDGSSWESGNSNQVCGR  NN+MKMSGF+L+QG PLF+SE
Sbjct: 248 QPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPLFISE 307

Query: 310 FGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGLLDWNWC 369
           FG+DQRG+NVNDNRYLSCFLAVAAEFDLDWA+WTLVGSYYLREGVVGLNEFYG+LDWNWC
Sbjct: 308 FGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILDWNWC 367

Query: 370 ALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGPCTDSDP 429
            LRNSTFLQRIS LQ+P QGPGLAERR+YN+IFHP +GLCVVRKSL+DPL+LGPC DSD 
Sbjct: 368 NLRNSTFLQRISVLQTPLQGPGLAERREYNLIFHPLSGLCVVRKSLLDPLRLGPCVDSDA 427

Query: 430 WYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKATDGSTV 489
           WYYTPQKFLTLKGTYFCIQADE+GKQAKLGIICTV+NAKWDMISDSK+HLSSK+++GS V
Sbjct: 428 WYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKSSNGSLV 487

Query: 490 CLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSSLANIVP 549
           CLDVD +TNE+VTN+CKCL+RDS+CDPSSQWFKLVNSTRSLG  RSMI+M GSSLAN+V 
Sbjct: 488 CLDVDANTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGGGRSMINMAGSSLANVVT 547

Query: 550 KFMEL 552
           KF+EL
Sbjct: 548 KFVEL 552

BLAST of Tan0013948 vs. ExPASy TrEMBL
Match: A0A1S3BF06 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103488945 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.4e-288
Identity = 465/545 (85.32%), Postives = 509/545 (93.39%), Query Frame = 0

Query: 10  IAAVW---LQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQPIDEIS 69
           + AVW     A VGLPLHTD+RWIVD EG+RVKLRCVNWV+HLEAVVAEGLSKQPI+EIS
Sbjct: 8   LLAVWCVAASAAVGLPLHTDTRWIVDGEGERVKLRCVNWVSHLEAVVAEGLSKQPIEEIS 67

Query: 70  NRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPVIQ 129
           NRI  LGFNCVRLTWPLFLATN+S NSLTVRQSFQRLGL EAIAGIQANNPFIIDLP+++
Sbjct: 68  NRIEGLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLTEAIAGIQANNPFIIDLPLLK 127

Query: 130 AFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNRMATMFN 189
           AFEAVVG+LGE KLMVILDNHISKPGWCCS+FDGNGFFGDQYFNP+ WIKGL RMATMFN
Sbjct: 128 AFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRMATMFN 187

Query: 190 GVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKDLSYLKT 249
           GV HVVAMSLRNELRGP+QNVNDWYRYMQRGAE VHSAN DIL+ILSGLSFD+DLS+LK 
Sbjct: 188 GVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSFDRDLSFLKN 247

Query: 250 QPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGMPLFVSE 309
           QPINLTFT K VYEVHWYAFSDGSSWESGNSNQVCGR  NN+MKMSGF+L+QG PLF+SE
Sbjct: 248 QPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPLFISE 307

Query: 310 FGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGLLDWNWC 369
           FG+DQRG+NVNDNRYLSCFLAVAAEFDLDWA+WTLVGSYYLREGVVGLNEFYG+LDWNWC
Sbjct: 308 FGIDQRGTNVNDNRYLSCFLAVAAEFDLDWALWTLVGSYYLREGVVGLNEFYGILDWNWC 367

Query: 370 ALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGPCTDSDP 429
            LRNSTFLQRIS LQ+P QGPGLAERR+YN+IFHP +GLCVVRKSL+DPL+LGPC DSD 
Sbjct: 368 NLRNSTFLQRISVLQTPLQGPGLAERREYNLIFHPLSGLCVVRKSLLDPLRLGPCVDSDA 427

Query: 430 WYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKATDGSTV 489
           WYYTPQKFLTLKGTYFCIQADE+GKQAKLGIICTV+NAKWDMISDSK+HLSSK+++GS V
Sbjct: 428 WYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKSSNGSLV 487

Query: 490 CLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSSLANIVP 549
           CLDVD +TNE+VTN+CKCL+RDS+CDPSSQWFKLVNSTRSLG  RSMI+M GSSLAN+V 
Sbjct: 488 CLDVDANTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGGGRSMINMAGSSLANVVT 547

Query: 550 KFMEL 552
           KF+EL
Sbjct: 548 KFVEL 552

BLAST of Tan0013948 vs. ExPASy TrEMBL
Match: A0A0A0KTH1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G593400 PE=3 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.6e-287
Identity = 464/545 (85.14%), Postives = 507/545 (93.03%), Query Frame = 0

Query: 10  IAAVW---LQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQPIDEIS 69
           I  VW     A VGLPLHTD+RWIVD  G+RVKLRCVNWV+HLEAVVAEGLSKQPI+EIS
Sbjct: 6   ILLVWCVAASAAVGLPLHTDTRWIVDGAGERVKLRCVNWVSHLEAVVAEGLSKQPIEEIS 65

Query: 70  NRIVSLGFNCVRLTWPLFLATNDSFNSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPVIQ 129
           NRI  LGFNCVRLTWPLFLATN+S NSLTVRQSFQRLGLAEAIAGIQANNPFIIDLP+++
Sbjct: 66  NRIQWLGFNCVRLTWPLFLATNESLNSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPLLK 125

Query: 130 AFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNRMATMFN 189
           AFEAVVG+LGE KLMVILDNHISKPGWCCS+FDGNGFFGDQYFNP+ WIKGL R+ATMFN
Sbjct: 126 AFEAVVGKLGEGKLMVILDNHISKPGWCCSNFDGNGFFGDQYFNPDLWIKGLTRIATMFN 185

Query: 190 GVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKDLSYLKT 249
           GV HVVAMSLRNELRGP+QNVNDWYRYMQRGAE VHSAN DIL+ILSGLS+D+DLS+LK 
Sbjct: 186 GVNHVVAMSLRNELRGPKQNVNDWYRYMQRGAEAVHSANPDILIILSGLSYDRDLSFLKN 245

Query: 250 QPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGMPLFVSE 309
           QPINLTFT K VYEVHWYAFSDGSSWESGNSNQVCGR  NN+MKMSGF+L+QG PLF+SE
Sbjct: 246 QPINLTFTSKTVYEVHWYAFSDGSSWESGNSNQVCGRTTNNLMKMSGFLLQQGFPLFISE 305

Query: 310 FGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGLLDWNWC 369
           FG+DQRG+NVNDNRYLSCFLAVAAE DLDWA+WTLVGSYYLREGVVGLNEFYG+LDWNWC
Sbjct: 306 FGIDQRGTNVNDNRYLSCFLAVAAELDLDWAVWTLVGSYYLREGVVGLNEFYGILDWNWC 365

Query: 370 ALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLKLGPCTDSDP 429
            LRNSTFLQRISALQSPFQGPGLAERR+YNVIFHP +GLCVVRKSL+DPL LGPC D+D 
Sbjct: 366 NLRNSTFLQRISALQSPFQGPGLAERREYNVIFHPLSGLCVVRKSLLDPLTLGPCVDTDA 425

Query: 430 WYYTPQKFLTLKGTYFCIQADEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKATDGSTV 489
           WYYTPQKFLTLKGTYFCIQADE+GKQAKLGIICTV+NAKWDMISDSK+HLSSK+++GS V
Sbjct: 426 WYYTPQKFLTLKGTYFCIQADEIGKQAKLGIICTVNNAKWDMISDSKLHLSSKSSNGSLV 485

Query: 490 CLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTRSMIDMVGSSLANIVP 549
           CLDVD STNE+VTN+CKCL+RDS+CDPSSQWFKLVNSTRSLG  RSMI+MVGSSL N+  
Sbjct: 486 CLDVDSSTNEIVTNSCKCLSRDSSCDPSSQWFKLVNSTRSLGRGRSMINMVGSSLPNVAT 545

Query: 550 KFMEL 552
           KF++L
Sbjct: 546 KFVDL 550

BLAST of Tan0013948 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 662.5 bits (1708), Expect = 2.9e-190
Identity = 307/535 (57.38%), Postives = 402/535 (75.14%), Query Frame = 0

Query: 4   FVSFCFIAAVWLQ---AGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQ 63
           F  FCF  +   Q     +  PL T SRWIVDE G RVKL C NW +HL+ VVAEGLSKQ
Sbjct: 13  FPFFCFFFSFIAQNTVPNMSYPLSTSSRWIVDENGLRVKLVCANWPSHLQPVVAEGLSKQ 72

Query: 64  PIDEISNRIVSLGFNCVRLTWPLFLATNDSF-NSLTVRQSFQRLGLAEAIAGIQANNPFI 123
           P+D ++ +IV +GFNCVRLTWPL L TN++  N++TVRQSFQ LGL + I G Q NNP I
Sbjct: 73  PVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSI 132

Query: 124 IDLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLN 183
           IDLP+I+A++ VV  LG   +MVILDNH++KPGWCC++ DGNGFFGDQ+F+P  W+  L 
Sbjct: 133 IDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALK 192

Query: 184 RMATMFNGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDK 243
           +MA  FNGV++VV MSLRNELRGP+QNVNDW++YMQ+GAE VHSAN+ +LVILSGLSFD 
Sbjct: 193 KMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDA 252

Query: 244 DLSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQG 303
           DLS+++++P+ L+FTGKLV+E+HWY+FSDG+SW + N N +CGRV+N I    G++L QG
Sbjct: 253 DLSFVRSRPVKLSFTGKLVFELHWYSFSDGNSWAANNPNDICGRVLNRIGNGGGYLLNQG 312

Query: 304 MPLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYG 363
            PLF+SEFG+D+RG N NDNRY  C    AAE D+DW++W L GSYYLR+G VG+NE+YG
Sbjct: 313 FPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYG 372

Query: 364 LLDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDP--LK 423
           +LD +W ++RNS+FLQ+IS LQSP QGPG      YN++FHP TGLC+VR SL DP  L 
Sbjct: 373 VLDSDWISVRNSSFLQKISFLQSPLQGPG-PRTDAYNLVFHPLTGLCIVR-SLDDPKMLT 432

Query: 424 LGPCTDSDPWYYTPQKFLTLKGTYFCIQADEMGKQAKL-GIICTVSNAKWDMISDSKMHL 483
           LGPC  S+PW YT +K L +K    C+Q++       +    C+ S +KW  IS S+MHL
Sbjct: 433 LGPCNSSEPWSYT-KKALRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHL 492

Query: 484 SSKATDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTRSLGVTR 532
           +S  ++ +++CLDVD + N +V NACKCL++D +C+P SQWFK++ +TR L  +R
Sbjct: 493 ASTTSNKTSLCLDVD-TANNVVANACKCLSKDKSCEPMSQWFKIIKATRPLKSSR 543

BLAST of Tan0013948 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 632.5 bits (1630), Expect = 3.2e-181
Identity = 298/531 (56.12%), Postives = 400/531 (75.33%), Query Frame = 0

Query: 1   MRRFVSFCFIAAVWLQAGVGLPLHTDSRWIVDE--EGQRVKLRCVNWVAHLEAVVAEGLS 60
           M +F         ++      P  TDSRWIVD+  +G+RVKL CVNW +HLE  VAEGLS
Sbjct: 1   MEKFFFISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLS 60

Query: 61  KQPIDEISNRIVSLGFNCVRLTWPLFLATNDSFNS-LTVRQSFQRLGLAEAIAGIQANNP 120
           KQP+D I+ +IVS+GFNCVRLTWPL+LAT++SF++ +TVRQS ++  L EA++G Q +NP
Sbjct: 61  KQPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNP 120

Query: 121 FIIDLPVIQAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKG 180
            I+DLP+I+AF+ VV  L + ++MVILDNHIS+PGWCCS  DGNGFFGD++ NP+ WIKG
Sbjct: 121 TILDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKG 180

Query: 181 LNRMATMFNGV-AHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLS 240
           L +MA+MF  V ++VV MSLRNELRGP+QN+ DWY+YM+ GAE VHS N ++LVI+SGL+
Sbjct: 181 LKKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLN 240

Query: 241 FDKDLSYLKTQPINLTFTGKLVYEVHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVL 300
           +  DLS+L+ +P  ++F  K+V+E+HWY F   ++WE  N N++CG+    +MKMSGF+L
Sbjct: 241 YATDLSFLRERPFEVSFRRKVVFEIHWYGF--WNTWEGDNLNKICGKETEKMMKMSGFLL 300

Query: 301 EQGMPLFVSEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNE 360
           E+G+PLFVSEFG+DQRG+N NDN++LSCF+A+AA+ DLDW++WTL GSYY+RE  +G +E
Sbjct: 301 EKGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDE 360

Query: 361 FYGLLDWNWCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPL 420
            YG+LD+NW ++RNST LQ ISA+Q+PF   GL E +   ++FHPSTGLC+VRKSL   L
Sbjct: 361 SYGVLDFNWSSIRNSTILQMISAIQTPF--IGLMETQPKKIMFHPSTGLCIVRKSLFQ-L 420

Query: 421 KLGPCTDSDPWYYTPQKFLTL-KGTYFCIQADEMGKQAKLGIICTVSN-AKWDMISDSKM 480
           KLG C  S+ W  +  + L+L +    C++A E GK  KL +  + S  +KW + SDSKM
Sbjct: 421 KLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFSDSKM 480

Query: 481 HLSSKATDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKLVNSTR 526
            LSS   +G +VCLDVD   N +VTN+CKCL  +S+CDP SQWFKLV STR
Sbjct: 481 QLSSITKNGFSVCLDVDTENNNIVTNSCKCLRGNSSCDPRSQWFKLVTSTR 526

BLAST of Tan0013948 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 608.6 bits (1568), Expect = 5.0e-174
Identity = 284/510 (55.69%), Postives = 389/510 (76.27%), Query Frame = 0

Query: 22  PLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQPIDEISNRIVSLGFNCVRLTW 81
           PL T+SRWI+DE+GQRVKL CVNW +HL+ VVAEGLSKQ +D+++ +I+++GFNCVR TW
Sbjct: 4   PLSTNSRWIIDEKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFTW 63

Query: 82  PLFLATNDSF-NSLTVRQSFQRLGLAEAIAGIQANNPFIIDLPVIQAFEAVVGRLGEAKL 141
           PL LATN++  N++TVRQSFQ LGL + I+G +  NP +IDLP+I+A++ VV +LG   +
Sbjct: 64  PLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNNV 123

Query: 142 MVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNRMATMFNGVAHVVAMSLRNEL 201
           MVILDNH++KPGWCC   DGNGFFGD +F+P TWI GL ++A  F G  +VV MSLRNEL
Sbjct: 124 MVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNEL 183

Query: 202 RGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKDLSYLKTQPINLTFTGKLVYE 261
           RGP+QNV+DW++YMQ+GAE VH AN ++LVILSGLS+D DLS+++++ +NLTFT KLV+E
Sbjct: 184 RGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLVFE 243

Query: 262 VHWYAFSDGSSWESGNSNQVCGRVVNNIMKMSGFVLEQGMPLFVSEFGVDQRGSNVNDNR 321
           +H Y+F++ ++W S N N+ CG ++ +I    GF L +  P+F+SEFG+D RG NVNDNR
Sbjct: 244 LHRYSFTNTNTWSSKNPNEACGEILKSIENGGGFNL-RDFPVFLSEFGIDLRGKNVNDNR 303

Query: 322 YLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGLLDWNWCALRNSTFLQRISAL 381
           Y+ C L  AAE D+DW+IWTL GSYYLREGVVG++EFYG+LD +W  +R+ +FLQR+S +
Sbjct: 304 YIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFLQRLSLI 363

Query: 382 QSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLIDPLK--LGPCTDSDPWYYTPQKFLTLK 441
            SP QGPG ++ + YN++FHP TGLC++ +S++DP K  LG C +S PW YTPQ  LTLK
Sbjct: 364 LSPLQGPG-SQSKVYNLVFHPLTGLCML-QSILDPTKVTLGLCNESQPWSYTPQNTLTLK 423

Query: 442 GTYFCIQADEMGKQAKLG-IICTVSN-AKWDMISDSKMHLSSKATDGSTVCLDVDPSTNE 501
               C+++       KL    C+  N ++W+ IS S M L++K+T+ S +CLDVD  TN 
Sbjct: 424 DKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTNNS-LCLDVD-ETNN 483

Query: 502 LVTNACKCLT-RDSTCDPSSQWFKLVNSTR 526
           L+ + CKC+   DS+CDP SQWFK+V  ++
Sbjct: 484 LMASNCKCVKGEDSSCDPISQWFKIVKVSK 508

BLAST of Tan0013948 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 574.3 bits (1479), Expect = 1.0e-163
Identity = 276/520 (53.08%), Postives = 363/520 (69.81%), Query Frame = 0

Query: 7   FCFIAAVWLQAGVGLPLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQPIDEIS 66
           F F++ + L      PL T SRWIV+ +G RVKL C NW +HL+ VVAEGLS QP+D IS
Sbjct: 12  FLFLSLISLTLATDYPLFTKSRWIVNNKGHRVKLACANWPSHLKPVVAEGLSSQPMDSIS 71

Query: 67  NRIVSLGFNCVRLTWPLFLATNDSFN-SLTVRQSFQRLGLAEAIAGIQANNPFIIDLPVI 126
            +I  +GFNCVRLTWPL L  ND+   ++TV+QSF+R GL   + GI  +NP+I++ P+I
Sbjct: 72  KKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVNTPLI 131

Query: 127 QAFEAVVGRLGEAKLMVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNRMATMF 186
             F+AVV  LG   +MVILDNH + PGWCCS+ D + FFGD  FNP+ W+ GL +MAT+F
Sbjct: 132 NVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKMATIF 191

Query: 187 NGVAHVVAMSLRNELRGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKDLSYLK 246
             V +VV MSLRNELRG      DWY+YMQ+GAE VH++N ++LVILSGL+FD DLS+LK
Sbjct: 192 MNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADLSFLK 251

Query: 247 TQPINLTFTGKLVYEVHWYAFSDGS-SWESGNSNQVCGRVVNNIMKMSGFVLEQGMPLFV 306
            +P+NL+F  KLV E+HWY+F+DG+  W+S N N  C ++ +   +  GFVL+QG PLF+
Sbjct: 252 DRPVNLSFKKKLVLELHWYSFTDGTGQWKSHNVNDFCSQMFSKERRTGGFVLDQGFPLFL 311

Query: 307 SEFGVDQRGSNVNDNRYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGLLDWN 366
           SEFG DQRG ++  NRY++C LA AAE DLDWA+W + G YY REG  G+ E YG+LD N
Sbjct: 312 SEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVEAYGMLDAN 371

Query: 367 WCALRNSTFLQRISALQSPFQGPGLAERRQYNVIFHPSTGLCVVRKSLI--DPLKLGPCT 426
           W  + N T+L+R+S +Q P  GPG+ +   +  IFHP TGLC+VRKS      L LGPCT
Sbjct: 372 WHNVHNYTYLRRLSVIQPPHTGPGV-KHNHHKKIFHPLTGLCLVRKSHCHESELTLGPCT 431

Query: 427 DSDPWYYTPQKFLTL-KGTYFCIQAD-EMGKQAKLGIICTVSNAKWDMISDSKMHLSSKA 486
             +PW Y+    L + +G   C++ +  +GK  KLG ICT    K + IS +KMHLS   
Sbjct: 432 KDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICT----KIEQISATKMHLSFNT 491

Query: 487 TDGSTVCLDVDPSTNELVTNACKCLTRDSTCDPSSQWFKL 521
           +DGS VCLDVD S N +V N+C CLT D+TC+P+SQWFK+
Sbjct: 492 SDGSLVCLDVD-SDNNVVANSCNCLTGDTTCEPASQWFKI 525

BLAST of Tan0013948 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 508.1 bits (1307), Expect = 9.2e-144
Identity = 252/505 (49.90%), Postives = 330/505 (65.35%), Query Frame = 0

Query: 22  PLHTDSRWIVDEEGQRVKLRCVNWVAHLEAVVAEGLSKQPIDEISNRIVSLGFNCVRLTW 81
           PL T SRWIVDE+GQRVKL CVNW AHL+  VAEGLSKQP+D IS +IVS+GFNCVRLTW
Sbjct: 25  PLSTKSRWIVDEKGQRVKLACVNWPAHLQPTVAEGLSKQPLDSISKKIVSMGFNCVRLTW 84

Query: 82  PLFLATNDSFN-SLTVRQSFQRLGLAEAIAGIQANNPFIIDLPVIQAFEAVVGRLGEAKL 141
           PL L TND+    +TV+QSF+ L L E + GIQ +NP ++ LP+  AF+ VV  LGE  +
Sbjct: 85  PLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLPLFNAFQEVVSNLGENGV 144

Query: 142 MVILDNHISKPGWCCSSFDGNGFFGDQYFNPETWIKGLNRMATMFNGVAHVVAMSLRNEL 201
           MVILDNH++ PGWCC   D + FFG  +F+P  W KGL +MAT+F    HV+ MSLRNE 
Sbjct: 145 MVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMATLFRNFTHVIGMSLRNEP 204

Query: 202 RGPRQNVNDWYRYMQRGAETVHSANSDILVILSGLSFDKDLSYLKTQPINLTFTGKLVYE 261
           RG R   + W+R+M +GAE VH+AN  +LVILSG+ FD +LS+L+ + +N++FT KLV+E
Sbjct: 205 RGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSFLRDRSVNVSFTDKLVFE 264

Query: 262 VHWYAFSDG-SSWESGNSNQVCGRVVNNIMKMSGFVLEQGMPLFVSEFGVDQRGSNVNDN 321
           +HWY+FSDG  SW   NSN  C +++  +    GF+L +G PL +SEFG DQRG +++ N
Sbjct: 265 LHWYSFSDGRDSWRKHNSNDFCVKIIEKVTHNGGFLLGRGFPLILSEFGTDQRGGDMSGN 324

Query: 322 RYLSCFLAVAAEFDLDWAIWTLVGSYYLREGVVGLNEFYGLLDWNWCALRNSTFLQRISA 381
           RY++C +A AAE DLDWA+W L G YYLR                               
Sbjct: 325 RYMNCLVAWAAENDLDWAVWALTGDYYLR------------------------------- 384

Query: 382 LQSPFQGPGLAERRQYNVIFHPSTGLCVVR--KSLIDPLKLGPCTDSDPWYYTPQKFLTL 441
                 GPGL  R   N++FHPSTGLCV       I  L+LGPC  SDPW + P + + L
Sbjct: 385 -----TGPGL--RPNKNLLFHPSTGLCVTNNPSDNIPTLRLGPCPKSDPWTFNPSEGI-L 444

Query: 442 KGTYFCIQA-DEMGKQAKLGIICTVSNAKWDMISDSKMHLSSKATDGSTVCLDVDPSTNE 501
                C++A + +G++ KLG+    S  K   IS +KMHLS K ++G  +CLDVD   N 
Sbjct: 445 WINKMCVEAPNVVGQKVKLGVGTKCS--KLGQISATKMHLSFKTSNGLLLCLDVDERDNS 488

Query: 502 LVTNACKCLTRDSTCDPSSQWFKLV 522
           +V N CK LT D++CDP+SQWFK++
Sbjct: 505 VVANRCKFLTMDASCDPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA04.3e-13046.58Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038893388.11.4e-30388.11glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
XP_023514558.15.3e-29586.86uncharacterized protein LOC111778811 [Cucurbita pepo subsp. pepo][more]
KAG6601558.15.9e-29486.68hypothetical protein SDJN03_06791, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7032321.12.9e-29386.50hypothetical protein SDJN02_06366, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022957392.18.5e-29386.31uncharacterized protein LOC111458804 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1GZ004.1e-29386.31Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111458804 P... [more]
A0A6J1KEP83.0e-29185.77Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111492584 PE=... [more]
A0A5D3CXK41.4e-28885.32Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BF061.4e-28885.32Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103488945 PE=3 SV... [more]
A0A0A0KTH17.6e-28785.14Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G593400 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G13130.12.9e-19057.38Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.13.2e-18156.12Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.15.0e-17455.69Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.11.0e-16353.08Glycosyl hydrolase superfamily protein [more]
AT5G16700.19.2e-14449.90Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 60..341
e-value: 2.3E-24
score: 86.3
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 21..382
e-value: 2.5E-74
score: 252.6
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 10..528
NoneNo IPR availablePANTHERPTHR31263:SF44CELLULASE (GLYCOSYL HYDROLASE FAMILY 5) PROTEINcoord: 10..528
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 395..502
e-value: 0.0049583
score: 35.5591
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 22..375
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 394..520

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0013948.1Tan0013948.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds