Tan0013463 (gene) Snake gourd v1

Overview
NameTan0013463
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionrRNA 2'-O-methyltransferase fibrillarin
LocationLG01: 1897788 .. 1901247 (-)
RNA-Seq ExpressionTan0013463
SyntenyTan0013463
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTTAAAACCCCAAACAAGAGCTCTTCACTTCGTCTTTGTGCTCTGCAGTTCTAAAACCCTAGCCGCAACTATCCTCCGGCGCTCGCTCTTTCGGTGACTGAAATGAGACCTCCTCGAGGTACAATCGTGAAACAAGCAGAGCTTGTACTCCTTTTGTTTTCGATTTCCTTGTTTTTGGATTTTGAAAATGCTCTGTTGAGTACGATGGAGACTAATGTTTGTAACGTAATGTATTGTTCGTCTGTTTGTTTCCGTTCTTACTACTTACTGAATCCACATCTATAAAATCTCGTTGATCGGTTGTTTGATTGTTGGCTCCAGCTATTCTACTTGCGTGTTTTATCACTTTGTATGGGTCATTAAATTGGAGTAAGTTGGATATGTGCAGGCCGCGGTGGTGGTGGTGGATTTAGGGGAAGGGGTGATGGAGGAAGGGGTGGTGGTAGAGGCCGAGGTGGTGGTGGTGGTAGAGGCGGCGGCGGAGGCAGAGGTATGCAAAGCCGCGGTGGTGGTCGCGGAGGTGGAAGAGGTCGCGGTGGAGGAAGAGGAGGTGGTATGAAAGGTGGAAGCAAAGTTGTCGTTGAACCCCACAGACACGAAGGTATTTTCATTGCCAAGGGTAAAGAAGATGCTCTTGTCACTAAGAACATGGTCTCTGGTGAATCTGTCTACAACGAGAAGAGAATATCCGTCCAGGTGATTCGCTTCTAGCAGTAATCCTGCCTTTTAATGCTTATGCCTAGATGTTTGTTAATTTTCAAGGTACACTTATTGTGGTTGAGGGAAACCATGCTGTTTTTGGAGAAGTTTGGTATGAGTTTTTTTTTTATTATTTAAATTATCCATGAGAAATTCTAGTTGACATTAGTATCTCCAAATAGTTTTGGCTGGTTCTGGCACGGTAGTTAGTGCTTGTAAAATATTTATCCTTAGTTAAGTGAATCGTCTATGCATGTTCTATTGTTATACCTTCAAAGCATTCCATTTTGCAAGTATTTGTGTTCGTAAACTGTCTGCATGGCTAATTGTTTGATGTACTACTGTCCTTCCAGAATGAAGATGGAACAAAAATAGAATACAGAGTTTGGAACCCATTCAGGTCTAAGTTGGCTGCTGCTGTTCTTGGTGGTGTTGATGACATATGGATCGTTAGTACTTTTCACCTCCAATTTGACGTCCTAATGTTTTATCTACTTAACTCTTTAGTTTGTCTTTTCTTTTAGTTATTTTTAACGTGTTAATGTATGTATTGTAGAAACCTGGTGCTCGAGTGTTGTATCTCGGTGCTGCTTCAGGAACAACTGTATCTCATGTCTCAGATGTTGTTGGCCCTGTAAGTAGTTATTTTGAGAGCATCTCTATTAAGTTTCATGAAGTAAAAAAGGTTTCTTAACATTTGTTGCTGCTTTGATTTGCAGACTGGTATAGTGTATGCCGTTGAATTCTCTCATAGGAGTGGTAGAGATTTAGTCAACATGGCAAAGAAGCGCACAAATGTTATACCAATCATTGAAGATGCTAGACACCCAGCCAAGTACCGAATGTTGGTTGGTATGGTGGATGTGATATTTTCAGATGTTGCTCAGCCAGATCAGGTTCACACTTCTTTCTTTTTCTGATTTGTCTGTATTATTCTGGTATGTGAACACCACCATCTTAGCTGAAATTTAAAGGCACAGTTCTTTGATTTATAAATAGAATCTAACTGCTGTTGTATCTTATTTCCCTGCATTAACCCGTTCTTATTTCCCTGTTTCGGATGTAATATCACTCTTTCATCGTTTATTCTTAAAGCCATTTTCCCCTTAAATTGCTTAGCATATGATGAGTTGCTCGGATTCCCCTTCAAGACTTCTTGAATGATGCAAAACCCGAGTTTCTGATGCTTGAAAATACTTAATTTTGAATTGGTATTATCTTTTGTTTTTTTTAATTATACTGCTCGTCTGATACTATCAACTTCTTTTTTTCCAACGTCTTTCTTGGATTTGTTTTCCTTTTTCCATTGTTGGTCATTTTTTATATTTCAAATGAATGGTATCAAATCCATGAAAGTTTTTTTTAATATTCTGTTTTAAAAATATTTTTCATTTTCTATTACTCATGAAATATTTTTGTTATCATATTTAACCATTCATTGTCATTTTATTAGTTCTTTGATTTAAACCACATTGTTTTCTTAGCGTGCGTAGCGGTAATCTGAATTTGAATTTATGATTCCCGTGCAAGAAATAGTAATCACAATCACCTCTACATTTTGACATTTACACCACCCATCCCACCTCTTTTAGCTCTATTTATATTTGGATTCTGTCGGTAGTTTAGTTGCAAGCTATTCTGCTCAATCAATATATGGGATCTCTCTATGCATAATCTTTCTAATTTCCCCCCAATAATCTCTTACCAAAAAATATTTCTCCCCAATAATCGTTTTATTACTTGCAGTTCTTTTTTTTTAATGATATGCTCTAACTATGGATTAGTTTGGTTTTGGAGGTTTAGATTCTAGGCTCTTCTGTAGAGTTTAAAGAACTAAAGAAGAAAATAACGAGAATCTACTATATCTTATTTAGATCATCTTTGTTTGGATAATGAGATATAATGGGGACTGTGCCACAAATCTTTAGCCCTCTCATCCATTTCAATACAGATTTATCTTCACTTAATAATTTGATTCTTACGTCCGGTTATCCTGCAGGTATATTTGCTATTGAACATAGTGCTATGCATCTTATCACATTTTTATCTTTTAGGCTCTTCCTCTTTGCTAAGCATGTGATGAATTACTCGGATTCCCCTTCAAGACATCTTGAATGATGCACAACCCGAGTTTCTGATGCGTCCAAGAATATCCCATTTTTATTAAACCTTCCATCAGTTAGCCATTTTTGCATTCTTGCTCTGCCAATTTTTTAAACCTATGAACTTGTCTGCATCTTACATCGACCTCTCTTAACTTCCTTTTTCAACGCAGGCAAGAATTTTGGCACTTAATGCATCATATTTTCTCAAAGCCGGTGGTCATTTTGTTATATCAATCAAGGTGAGTGTATGGTTTCCATTTTGTTAAAGATGAAAGACCGTGCATAATCCATGAATAGAATTTGATATATTTCATATTGAACTTTGTAGGCCAACTGTATCGACTCGACTGTCCCAGCAGAGGCAGTGTTTGCAAGTGAAGTGAATAAGCTAAAGGCAGACCAATTCAAACCCACCGAGCAAGTGACTCTCGAACCATTCGAGCGAGACCATGCTTGTGTTGTTGGTATCTATCGTGCGCTGAAGAAACAGAAGCCCGCTGCTTAGACAGTTGAACGAACAGCAGGATTTGGATTCTTATGGATGATTTATAGATAGTTCACAGCTGTAGCTTATCCATGTTTCTTATTTTGCTAGCTTGTAGCCGAGATCATCTTGAAAACCAATATGCGAGTGAACCTTTTTTTTTTTTTTT

mRNA sequence

AAATTTAAAACCCCAAACAAGAGCTCTTCACTTCGTCTTTGTGCTCTGCAGTTCTAAAACCCTAGCCGCAACTATCCTCCGGCGCTCGCTCTTTCGGTGACTGAAATGAGACCTCCTCGAGGCCGCGGTGGTGGTGGTGGATTTAGGGGAAGGGGTGATGGAGGAAGGGGTGGTGGTAGAGGCCGAGGTGGTGGTGGTGGTAGAGGCGGCGGCGGAGGCAGAGGTATGCAAAGCCGCGGTGGTGGTCGCGGAGGTGGAAGAGGTCGCGGTGGAGGAAGAGGAGGTGGTATGAAAGGTGGAAGCAAAGTTGTCGTTGAACCCCACAGACACGAAGGTATTTTCATTGCCAAGGGTAAAGAAGATGCTCTTGTCACTAAGAACATGGTCTCTGGTGAATCTGTCTACAACGAGAAGAGAATATCCGTCCAGAATGAAGATGGAACAAAAATAGAATACAGAGTTTGGAACCCATTCAGGTCTAAGTTGGCTGCTGCTGTTCTTGGTGGTGTTGATGACATATGGATCAAACCTGGTGCTCGAGTGTTGTATCTCGGTGCTGCTTCAGGAACAACTGTATCTCATGTCTCAGATGTTGTTGGCCCTACTGGTATAGTGTATGCCGTTGAATTCTCTCATAGGAGTGGTAGAGATTTAGTCAACATGGCAAAGAAGCGCACAAATGTTATACCAATCATTGAAGATGCTAGACACCCAGCCAAGTACCGAATGTTGGTTGGTATGGTGGATGTGATATTTTCAGATGTTGCTCAGCCAGATCAGGCAAGAATTTTGGCACTTAATGCATCATATTTTCTCAAAGCCGGTGGTCATTTTGTTATATCAATCAAGGCCAACTGTATCGACTCGACTGTCCCAGCAGAGGCAGTGTTTGCAAGTGAAGTGAATAAGCTAAAGGCAGACCAATTCAAACCCACCGAGCAAGTGACTCTCGAACCATTCGAGCGAGACCATGCTTGTGTTGTTGGTATCTATCGTGCGCTGAAGAAACAGAAGCCCGCTGCTTAGACAGTTGAACGAACAGCAGGATTTGGATTCTTATGGATGATTTATAGATAGTTCACAGCTGTAGCTTATCCATGTTTCTTATTTTGCTAGCTTGTAGCCGAGATCATCTTGAAAACCAATATGCGAGTGAACCTTTTTTTTTTTTTTT

Coding sequence (CDS)

ATGAGACCTCCTCGAGGCCGCGGTGGTGGTGGTGGATTTAGGGGAAGGGGTGATGGAGGAAGGGGTGGTGGTAGAGGCCGAGGTGGTGGTGGTGGTAGAGGCGGCGGCGGAGGCAGAGGTATGCAAAGCCGCGGTGGTGGTCGCGGAGGTGGAAGAGGTCGCGGTGGAGGAAGAGGAGGTGGTATGAAAGGTGGAAGCAAAGTTGTCGTTGAACCCCACAGACACGAAGGTATTTTCATTGCCAAGGGTAAAGAAGATGCTCTTGTCACTAAGAACATGGTCTCTGGTGAATCTGTCTACAACGAGAAGAGAATATCCGTCCAGAATGAAGATGGAACAAAAATAGAATACAGAGTTTGGAACCCATTCAGGTCTAAGTTGGCTGCTGCTGTTCTTGGTGGTGTTGATGACATATGGATCAAACCTGGTGCTCGAGTGTTGTATCTCGGTGCTGCTTCAGGAACAACTGTATCTCATGTCTCAGATGTTGTTGGCCCTACTGGTATAGTGTATGCCGTTGAATTCTCTCATAGGAGTGGTAGAGATTTAGTCAACATGGCAAAGAAGCGCACAAATGTTATACCAATCATTGAAGATGCTAGACACCCAGCCAAGTACCGAATGTTGGTTGGTATGGTGGATGTGATATTTTCAGATGTTGCTCAGCCAGATCAGGCAAGAATTTTGGCACTTAATGCATCATATTTTCTCAAAGCCGGTGGTCATTTTGTTATATCAATCAAGGCCAACTGTATCGACTCGACTGTCCCAGCAGAGGCAGTGTTTGCAAGTGAAGTGAATAAGCTAAAGGCAGACCAATTCAAACCCACCGAGCAAGTGACTCTCGAACCATTCGAGCGAGACCATGCTTGTGTTGTTGGTATCTATCGTGCGCTGAAGAAACAGAAGCCCGCTGCTTAG

Protein sequence

MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALKKQKPAA
Homology
BLAST of Tan0013463 vs. ExPASy Swiss-Prot
Match: Q94AH9 (rRNA 2'-O-methyltransferase fibrillarin 2 OS=Arabidopsis thaliana OX=3702 GN=FIB2 PE=1 SV=2)

HSP 1 Score: 443.7 bits (1140), Expect = 1.6e-123
Identity = 260/317 (82.02%), Postives = 272/317 (85.80%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGG-GGGRGG---GGGRGMQSRGG-GRGGGRGRG 60
           MRPP    GGG   GRG GG  GGRG GG  GGRGG   GGGRG   RGG GRG G  RG
Sbjct: 1   MRPPLTGSGGGFSGGRGRGGYSGGRGDGGFSGGRGGGGRGGGRGFSDRGGRGRGRGPPRG 60

Query: 61  GGRG-------GGMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQ 120
           G RG       GGMKGGSKV+VEPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQ
Sbjct: 61  GARGGRGPAGRGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQ 120

Query: 121 NEDGTKIEYRVWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTG 180
           NEDGTK EYRVWNPFRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G
Sbjct: 121 NEDGTKTEYRVWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEG 180

Query: 181 IVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARI 240
            VYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARI
Sbjct: 181 CVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARI 240

Query: 241 LALNASYFLKAGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERD 300
           LALNASYFLK+GGHFVISIKANCIDSTVPAEAVF +EV KL+ +QFKP EQVTLEPFERD
Sbjct: 241 LALNASYFLKSGGHFVISIKANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERD 300

Query: 301 HACVVGIYRALKKQKPA 306
           HACVVG YR  KK K A
Sbjct: 301 HACVVGGYRMPKKPKAA 317

BLAST of Tan0013463 vs. ExPASy Swiss-Prot
Match: Q9FEF8 (rRNA 2'-O-methyltransferase fibrillarin 1 OS=Arabidopsis thaliana OX=3702 GN=FIB1 PE=1 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.8e-122
Identity = 256/308 (83.12%), Postives = 268/308 (87.01%), Query Frame = 0

Query: 1   MRPP--RGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGR 60
           MRPP   GRGGGG   GR  GGRG G GR  GGGR G  GR    RG GRG  RGRGG  
Sbjct: 1   MRPPVTGGRGGGGFRGGRDGGGRGFGGGRSFGGGRSGDRGRS-GPRGRGRGAPRGRGGPP 60

Query: 61  GGGMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYR 120
            GGMKGGSKV+VEPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTK+EYR
Sbjct: 61  RGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYR 120

Query: 121 VWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHR 180
           VWNPFRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G VYAVEFSHR
Sbjct: 121 VWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHR 180

Query: 181 SGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLK 240
           SGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS+FLK
Sbjct: 181 SGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASFFLK 240

Query: 241 AGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA 300
            GGHFVISIKANCIDSTV AEAVF SEV KL+ +QFKP EQVTLEPFERDHACVVG YR 
Sbjct: 241 TGGHFVISIKANCIDSTVAAEAVFQSEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRM 300

Query: 301 LKKQKPAA 307
            KKQK  A
Sbjct: 301 PKKQKTPA 307

BLAST of Tan0013463 vs. ExPASy Swiss-Prot
Match: Q8I1F4 (rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila erecta OX=7220 GN=Fib PE=3 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 6.7e-109
Identity = 227/297 (76.43%), Postives = 251/297 (84.51%), Query Frame = 0

Query: 6   GRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGG 65
           GRGG GG RG G  G GGG GRGG GGRGGGGGRG   RGGG  GG GRGGG  GG KGG
Sbjct: 51  GRGGFGGGRGGGGRGGGGGGGRGGFGGRGGGGGRGGGGRGGGGRGGGGRGGG-AGGFKGG 110

Query: 66  SKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRS 125
             V +EPHRHEG+FIA+GKEDALVT+N V G  VY EKRISV+N +G KIEYRVWNPFRS
Sbjct: 111 KTVTIEPHRHEGVFIARGKEDALVTRNFVPGSEVYGEKRISVEN-NGEKIEYRVWNPFRS 170

Query: 126 KLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVN 185
           KLAAAVLGGV+ I + PG++VLYLGAASGTTVSHVSDVVGP G+VYAVEFSHRSGRDL+N
Sbjct: 171 KLAAAVLGGVEQIHMPPGSKVLYLGAASGTTVSHVSDVVGPEGLVYAVEFSHRSGRDLIN 230

Query: 186 MAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVI 245
           +AKKRTN+IPIIEDARHP KYRMLVGMVD IF+DVAQPDQ RI+ALNA +FLK GGHFVI
Sbjct: 231 VAKKRTNIIPIIEDARHPHKYRMLVGMVDTIFADVAQPDQGRIVALNAQHFLKNGGHFVI 290

Query: 246 SIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALKKQ 303
           SIKA+CIDST   EAVFASEV K++AD+ KP EQ+TLEP+ERDHA VVG+YR   KQ
Sbjct: 291 SIKASCIDSTAQPEAVFASEVKKMQADKLKPQEQLTLEPYERDHAVVVGVYRPPPKQ 345

BLAST of Tan0013463 vs. ExPASy Swiss-Prot
Match: Q9W1V3 (rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila melanogaster OX=7227 GN=Fib PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 3.7e-107
Identity = 224/297 (75.42%), Postives = 249/297 (83.84%), Query Frame = 0

Query: 6   GRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGG 65
           GRGG GG RG G  G GGG GRG  GGRGGGGGRG   RGGG  GG GRGGG  GG KGG
Sbjct: 50  GRGGFGGGRGGGGRGGGGGGGRGAFGGRGGGGGRGGGGRGGGGRGGGGRGGG-AGGFKGG 109

Query: 66  SKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRS 125
             V +EPHRHEG+FIA+GKEDALVT+N V G  VY EKRISV+  +G KIEYRVWNPFRS
Sbjct: 110 KTVTIEPHRHEGVFIARGKEDALVTRNFVPGSEVYGEKRISVET-NGEKIEYRVWNPFRS 169

Query: 126 KLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVN 185
           KLAAAVLGGV+ I + PG++VLYLGAASGTTVSHVSDVVGP G+VYAVEFSHRSGRDL+N
Sbjct: 170 KLAAAVLGGVEQIHMPPGSKVLYLGAASGTTVSHVSDVVGPEGLVYAVEFSHRSGRDLIN 229

Query: 186 MAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVI 245
           +AKKRTN+IPIIEDARHP KYRMLVGMVD IF+DVAQPDQ RI+ALNA +FLK GGHFVI
Sbjct: 230 VAKKRTNIIPIIEDARHPHKYRMLVGMVDTIFADVAQPDQGRIVALNAQHFLKNGGHFVI 289

Query: 246 SIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALKKQ 303
           SIKA+CIDST   EAVFA+EV K++AD+ KP EQ+TLEP+ERDHA VVG+YR   KQ
Sbjct: 290 SIKASCIDSTAQPEAVFAAEVKKMQADKLKPQEQLTLEPYERDHAVVVGVYRPPPKQ 344

BLAST of Tan0013463 vs. ExPASy Swiss-Prot
Match: P22509 (rRNA 2'-O-methyltransferase fibrillarin OS=Rattus norvegicus OX=10116 GN=Fbl PE=1 SV=2)

HSP 1 Score: 384.0 bits (985), Expect = 1.6e-105
Identity = 225/304 (74.01%), Postives = 251/304 (82.57%), Query Frame = 0

Query: 6   GRGGGGGFRGRGDGGRGGGRGRGGGG----GRGGGGGRGMQSRGGGRGGGRGRGGGRGG- 65
           GRGG GGF G   G  GGGRGRGGGG    GRGGGGGRG    G   GGGRGRGGGRGG 
Sbjct: 27  GRGGRGGFGGGRGGFGGGGRGRGGGGGGFRGRGGGGGRG---GGFQSGGGRGRGGGRGGK 86

Query: 66  -GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRV 125
            G + G  V+VEPHRHEG+FI +GKEDALVTKN+V GESVY EKR+S+ +E   KIEYR 
Sbjct: 87  RGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSI-SEGDDKIEYRA 146

Query: 126 WNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRS 185
           WNPFRSKLAAA+LGGVD I IKPGA+VLYLGAASGTTVSHVSD+VGP G+VYAVEFSHRS
Sbjct: 147 WNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRS 206

Query: 186 GRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKA 245
           GRDL+N+AKKRTN+IP+IEDARHP KYRML+ MVDVIF+DVAQPDQ RI+ALNA  FL+ 
Sbjct: 207 GRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRN 266

Query: 246 GGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAL 304
           GGHFVISIKANCIDST  AEAVFASEV K++ +  KP EQ+TLEP+ERDHA VVG+YR  
Sbjct: 267 GGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPP 326

BLAST of Tan0013463 vs. NCBI nr
Match: XP_022141240.1 (mediator of RNA polymerase II transcription subunit 36a-like [Momordica charantia])

HSP 1 Score: 550.1 bits (1416), Expect = 1.2e-152
Identity = 301/306 (98.37%), Postives = 303/306 (99.02%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRGGGGGRGM SRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVW
Sbjct: 61  GMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQKPAA
Sbjct: 301 KQKPAA 305

BLAST of Tan0013463 vs. NCBI nr
Match: XP_008439295.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 36a-like [Cucumis melo])

HSP 1 Score: 548.9 bits (1413), Expect = 2.8e-152
Identity = 302/306 (98.69%), Postives = 303/306 (99.02%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMV+GESVYNEKRISVQNEDGTKIEYRVW
Sbjct: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVTGESVYNEKRISVQNEDGTKIEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQK AA
Sbjct: 301 KQKAAA 305

BLAST of Tan0013463 vs. NCBI nr
Match: XP_023552409.1 (mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.4 bits (1409), Expect = 8.1e-152
Identity = 301/306 (98.37%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRGGGGGRGM SRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVW
Sbjct: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQK AA
Sbjct: 301 KQKAAA 305

BLAST of Tan0013463 vs. NCBI nr
Match: XP_022945875.1 (probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita moschata] >XP_022968445.1 probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita maxima] >KAG6574193.1 rRNA 2'-O-methyltransferase fibrillarin 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 545.8 bits (1405), Expect = 2.4e-151
Identity = 301/306 (98.37%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRG GGGRGM SRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGFGGGRGMASRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW
Sbjct: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGP+GIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQKPAA
Sbjct: 301 KQKPAA 305

BLAST of Tan0013463 vs. NCBI nr
Match: XP_022984980.1 (probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita maxima] >KAG7014755.1 Mediator of RNA polymerase II transcription subunit 36a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 542.7 bits (1397), Expect = 2.0e-150
Identity = 301/306 (98.37%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRGGGGGRGM SRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGGGGGRGM-SRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVW
Sbjct: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQK AA
Sbjct: 301 KQKAAA 304

BLAST of Tan0013463 vs. ExPASy TrEMBL
Match: A0A6J1CHH3 (mediator of RNA polymerase II transcription subunit 36a-like OS=Momordica charantia OX=3673 GN=LOC111011688 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.0e-153
Identity = 301/306 (98.37%), Postives = 303/306 (99.02%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRGGGGGRGM SRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVW
Sbjct: 61  GMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQKPAA
Sbjct: 301 KQKPAA 305

BLAST of Tan0013463 vs. ExPASy TrEMBL
Match: A0A1S3AY25 (mediator of RNA polymerase II transcription subunit 36a-like OS=Cucumis melo OX=3656 GN=LOC103484122 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.3e-152
Identity = 302/306 (98.69%), Postives = 303/306 (99.02%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMV+GESVYNEKRISVQNEDGTKIEYRVW
Sbjct: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVTGESVYNEKRISVQNEDGTKIEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQK AA
Sbjct: 301 KQKAAA 305

BLAST of Tan0013463 vs. ExPASy TrEMBL
Match: A0A6J1HX74 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita maxima OX=3661 GN=LOC111467690 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.1e-151
Identity = 301/306 (98.37%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRG GGGRGM SRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGFGGGRGMASRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW
Sbjct: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGP+GIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQKPAA
Sbjct: 301 KQKPAA 305

BLAST of Tan0013463 vs. ExPASy TrEMBL
Match: A0A6J1G280 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita moschata OX=3662 GN=LOC111449984 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.1e-151
Identity = 301/306 (98.37%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRG GGGRGM SRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGFGGGRGMASRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW
Sbjct: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGP+GIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQKPAA
Sbjct: 301 KQKPAA 305

BLAST of Tan0013463 vs. ExPASy TrEMBL
Match: A0A6J1JA27 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita maxima OX=3661 GN=LOC111483086 PE=3 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 9.6e-151
Identity = 301/306 (98.37%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGG 60
           MRPPRGRGGGGGFRGRGDGGR GGRGRGGGGGRGGGGGRGM SRGGGRGGGRGRGGGRGG
Sbjct: 1   MRPPRGRGGGGGFRGRGDGGR-GGRGRGGGGGRGGGGGRGM-SRGGGRGGGRGRGGGRGG 60

Query: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVW 120
           GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVW
Sbjct: 61  GMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVW 120

Query: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180
           NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG
Sbjct: 121 NPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSG 180

Query: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240
           RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG
Sbjct: 181 RDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAG 240

Query: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALK 300
           GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA K
Sbjct: 241 GHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPK 300

Query: 301 KQKPAA 307
           KQK AA
Sbjct: 301 KQKAAA 304

BLAST of Tan0013463 vs. TAIR 10
Match: AT4G25630.1 (fibrillarin 2 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-124
Identity = 260/317 (82.02%), Postives = 272/317 (85.80%), Query Frame = 0

Query: 1   MRPPRGRGGGGGFRGRGDGGRGGGRGRGG-GGGRGG---GGGRGMQSRGG-GRGGGRGRG 60
           MRPP    GGG   GRG GG  GGRG GG  GGRGG   GGGRG   RGG GRG G  RG
Sbjct: 1   MRPPLTGSGGGFSGGRGRGGYSGGRGDGGFSGGRGGGGRGGGRGFSDRGGRGRGRGPPRG 60

Query: 61  GGRG-------GGMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQ 120
           G RG       GGMKGGSKV+VEPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQ
Sbjct: 61  GARGGRGPAGRGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQ 120

Query: 121 NEDGTKIEYRVWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTG 180
           NEDGTK EYRVWNPFRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G
Sbjct: 121 NEDGTKTEYRVWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEG 180

Query: 181 IVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARI 240
            VYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARI
Sbjct: 181 CVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARI 240

Query: 241 LALNASYFLKAGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERD 300
           LALNASYFLK+GGHFVISIKANCIDSTVPAEAVF +EV KL+ +QFKP EQVTLEPFERD
Sbjct: 241 LALNASYFLKSGGHFVISIKANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERD 300

Query: 301 HACVVGIYRALKKQKPA 306
           HACVVG YR  KK K A
Sbjct: 301 HACVVGGYRMPKKPKAA 317

BLAST of Tan0013463 vs. TAIR 10
Match: AT5G52470.1 (fibrillarin 1 )

HSP 1 Score: 440.3 bits (1131), Expect = 1.3e-123
Identity = 256/308 (83.12%), Postives = 268/308 (87.01%), Query Frame = 0

Query: 1   MRPP--RGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGR 60
           MRPP   GRGGGG   GR  GGRG G GR  GGGR G  GR    RG GRG  RGRGG  
Sbjct: 1   MRPPVTGGRGGGGFRGGRDGGGRGFGGGRSFGGGRSGDRGRS-GPRGRGRGAPRGRGGPP 60

Query: 61  GGGMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYR 120
            GGMKGGSKV+VEPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTK+EYR
Sbjct: 61  RGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYR 120

Query: 121 VWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHR 180
           VWNPFRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G VYAVEFSHR
Sbjct: 121 VWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHR 180

Query: 181 SGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLK 240
           SGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS+FLK
Sbjct: 181 SGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASFFLK 240

Query: 241 AGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRA 300
            GGHFVISIKANCIDSTV AEAVF SEV KL+ +QFKP EQVTLEPFERDHACVVG YR 
Sbjct: 241 TGGHFVISIKANCIDSTVAAEAVFQSEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRM 300

Query: 301 LKKQKPAA 307
            KKQK  A
Sbjct: 301 PKKQKTPA 307

BLAST of Tan0013463 vs. TAIR 10
Match: AT5G52490.1 (Fibrillarin family protein )

HSP 1 Score: 338.2 bits (866), Expect = 6.9e-93
Identity = 195/287 (67.94%), Postives = 225/287 (78.40%), Query Frame = 0

Query: 25  RGRGGG--GGRG-GGGGRGMQSRGGGRGG--GRGRGGGR-----GGGMKGGSKVVVEPHR 84
           RGRGGG  GGRG   GG G   RG GRGG  GRGRG GR      GG+KGGSKV+V PHR
Sbjct: 6   RGRGGGVRGGRGLARGGEGSAVRGSGRGGESGRGRGPGRVKSESDGGIKGGSKVLVTPHR 65

Query: 85  HEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRSKLAAAVLGG 144
           H G+F+AK K DALVTKN+V GE +YNEKRI VQNED + +EYRVWNP RSKLA A+  G
Sbjct: 66  HAGVFVAKSKADALVTKNLVPGEIIYNEKRIFVQNEDRSTVEYRVWNPHRSKLADAITTG 125

Query: 145 VDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNMAKKRTNVI 204
           VD+IWIKPG +VLYLGA+SG TVSHVSD+VGP G VYAVE S   G+ L+NMA+KRTNVI
Sbjct: 126 VDNIWIKPGVKVLYLGASSGYTVSHVSDIVGPEGCVYAVEHSDICGKVLMNMAEKRTNVI 185

Query: 205 PIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVISIKANCIDS 264
           PIIEDARHPAKYRMLVGMVD+IFSDV  P+QA IL+LNASYFLK+GGHF+ISIKAN IDS
Sbjct: 186 PIIEDARHPAKYRMLVGMVDIIFSDVNHPEQANILSLNASYFLKSGGHFMISIKANSIDS 245

Query: 265 TVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRALKK 302
           T+ AE V+  EV KL+ ++ +PTE + L+  E  HACV G YR  +K
Sbjct: 246 TIAAETVYQMEVEKLQMEELRPTEILHLDSCEEKHACVFGGYRLPRK 292

BLAST of Tan0013463 vs. TAIR 10
Match: AT5G52470.2 (fibrillarin 1 )

HSP 1 Score: 312.4 bits (799), Expect = 4.1e-85
Identity = 189/227 (83.26%), Postives = 198/227 (87.22%), Query Frame = 0

Query: 1   MRPP--RGRGGGGGFRGRGDGGRGGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGR 60
           MRPP   GRGGGG   GR  GGRG G GR  GGGR G  GR    RG GRG  RGRGG  
Sbjct: 1   MRPPVTGGRGGGGFRGGRDGGGRGFGGGRSFGGGRSGDRGRS-GPRGRGRGAPRGRGGPP 60

Query: 61  GGGMKGGSKVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYR 120
            GGMKGGSKV+VEPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTK+EYR
Sbjct: 61  RGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYR 120

Query: 121 VWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHR 180
           VWNPFRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G VYAVEFSHR
Sbjct: 121 VWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHR 180

Query: 181 SGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQ 226
           SGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQ
Sbjct: 181 SGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQ 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AH91.6e-12382.02rRNA 2'-O-methyltransferase fibrillarin 2 OS=Arabidopsis thaliana OX=3702 GN=FIB... [more]
Q9FEF81.8e-12283.12rRNA 2'-O-methyltransferase fibrillarin 1 OS=Arabidopsis thaliana OX=3702 GN=FIB... [more]
Q8I1F46.7e-10976.43rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila erecta OX=7220 GN=Fib PE=3... [more]
Q9W1V33.7e-10775.42rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila melanogaster OX=7227 GN=Fi... [more]
P225091.6e-10574.01rRNA 2'-O-methyltransferase fibrillarin OS=Rattus norvegicus OX=10116 GN=Fbl PE=... [more]
Match NameE-valueIdentityDescription
XP_022141240.11.2e-15298.37mediator of RNA polymerase II transcription subunit 36a-like [Momordica charanti... [more]
XP_008439295.12.8e-15298.69PREDICTED: mediator of RNA polymerase II transcription subunit 36a-like [Cucumis... [more]
XP_023552409.18.1e-15298.37mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo sub... [more]
XP_022945875.12.4e-15198.37probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita mosc... [more]
XP_022984980.12.0e-15098.37probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita maxi... [more]
Match NameE-valueIdentityDescription
A0A6J1CHH36.0e-15398.37mediator of RNA polymerase II transcription subunit 36a-like OS=Momordica charan... [more]
A0A1S3AY251.3e-15298.69mediator of RNA polymerase II transcription subunit 36a-like OS=Cucumis melo OX=... [more]
A0A6J1HX741.1e-15198.37probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita ma... [more]
A0A6J1G2801.1e-15198.37probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita mo... [more]
A0A6J1JA279.6e-15198.37probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita ma... [more]
Match NameE-valueIdentityDescription
AT4G25630.11.2e-12482.02fibrillarin 2 [more]
AT5G52470.11.3e-12383.12fibrillarin 1 [more]
AT5G52490.16.9e-9367.94Fibrillarin family protein [more]
AT5G52470.24.1e-8583.26fibrillarin 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000692FibrillarinPRINTSPR00052FIBRILLARINcoord: 116..135
score: 75.71
coord: 168..185
score: 72.22
coord: 143..164
score: 79.22
coord: 193..222
score: 70.24
coord: 223..252
score: 67.62
coord: 275..296
score: 61.36
coord: 88..107
score: 64.29
IPR000692FibrillarinSMARTSM01206Fibrillarin_2coord: 70..298
e-value: 6.6E-173
score: 590.4
IPR000692FibrillarinPIRSFPIRSF006540Nop17pcoord: 19..300
e-value: 3.5E-121
score: 401.2
IPR000692FibrillarinPFAMPF01269Fibrillarincoord: 70..297
e-value: 9.7E-110
score: 365.0
IPR000692FibrillarinHAMAPMF_00351RNA_methyltransf_FlpAcoord: 84..297
score: 36.932789
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 65..120
e-value: 1.9E-29
score: 103.3
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 121..298
e-value: 1.6E-88
score: 296.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..67
NoneNo IPR availablePANTHERPTHR10335RRNA 2-O-METHYLTRANSFERASE FIBRILLARINcoord: 4..305
NoneNo IPR availablePANTHERPTHR10335:SF22FIBRILLARIN, S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE-RELATEDcoord: 4..305
IPR020813Fibrillarin, conserved sitePROSITEPS00566FIBRILLARINcoord: 168..182
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 68..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0013463.1Tan0013463.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000494 box C/D RNA 3'-end processing
biological_process GO:1990258 histone glutamine methylation
biological_process GO:0051321 meiotic cell cycle
biological_process GO:0007020 microtubule nucleation
biological_process GO:0000278 mitotic cell cycle
biological_process GO:0031167 rRNA methylation
biological_process GO:0051225 spindle assembly
biological_process GO:0006364 rRNA processing
biological_process GO:0031122 cytoplasmic microtubule organization
cellular_component GO:0005730 nucleolus
cellular_component GO:0000922 spindle pole
cellular_component GO:0032040 small-subunit processome
cellular_component GO:0000930 gamma-tubulin complex
cellular_component GO:0005874 microtubule
cellular_component GO:0000923 equatorial microtubule organizing center
cellular_component GO:0005737 cytoplasm
cellular_component GO:0031428 box C/D RNP complex
molecular_function GO:0043015 gamma-tubulin binding
molecular_function GO:1990259 histone-glutamine methyltransferase activity
molecular_function GO:0051011 microtubule minus-end binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0008649 rRNA methyltransferase activity
molecular_function GO:0008168 methyltransferase activity