Tan0013363 (gene) Snake gourd v1

Overview


Name	Tan0013363
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	EF-hand domain-containing protein
Location	LG01: 16346578 .. 16348779 (-)
RNA-Seq Expression	Tan0013363
Synteny	Tan0013363

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCCCACATATAAATAAGGCAATAAAATATAAAATTCATAAACATGTGCCCGACCAATAAGCAATAATCGACCGTGTATTTGGTATAGGCACTAAAAAAATCCGAGAACGGCAGAACCCCAATCGCATCTTGCTGAAGAAAATGACGATTATACCCCTGCAACGCGCCTTCTTCGAAATGACATAGCCTGCAAATCCGAATCAGCGTAACCAATCTACATTATTATCTGTACCTTAGTTGGCTTCCATGGCCGCTCCTTCCCCGAAAACCCCATTGACCTTCTTCTTCTTCACTCTTCCCCCATATATATCCCCCATTCCACTCCCTCTTCTCACACTCAAACCAAAGCCCTAATCCCTAATTCCTAATTCCAATCCCATCTTACTTCTCGCTCCAAATTTCCAATGGCTTTTGCTGCAAGTGCAAACCAATCGGGATTCGAGGACTGGCTGCCGACGATGGCGCAGAGGCTGGGCGGCGAGGGTTTGATTGGGGAGCTTTGCAATGGGTTCAATCTTCTGATGGACAGAGAGAAGGGGGTGATCGATTTCCAGAGCTTGAAGAGGAACGCGGCGGCGCTAGGGCTGGGGGATCTGAGCGATGAGGATCTCAGAGCGATGTTGAGGGAGGGGGATTTCGACGGCGACGGCGCTCTCAATCAGATGGAGTTTTGTGTTCTTATGTTCAGATTGAGCCCTGAATTGATGGAAGAGTCCTGCTTCTTGCTCGAGGAGGCTCTAGGCCATGAGTTTAAGCATTTTTGTTGATTTCTTTTTCTTTTGTGTAATTAATTACTGATTTGATTTAGTGAATATACTTGGAAAACAAAATTAAAAGAACAAATTGTTACGATTTTTCTTTTTGCCACTTTCAATTTCATCCAATTAACATGGCAACTTCAGTTTTCACTAAACCTACTCCAAATTTTACAAATAAGTAAAAATTTTCTTTAAACAACTTAAGAACAACAAAATTAATCAACACAAGATCCACCTGAAATAAATCTTTCAAAATTAATATATTTCAATATAAATTCAACTCTAGGGCTATTTTTTAAACTATTGCACTGAACATATTTTATAATTACTGAATGCACTTAAGAATATTTTTCTTGAGGATTTTTCAAAATGCAATTATAACATTTTTTCAAATTTAAAGTTAAAGATGGTCAAATATTTGTTTCACATACACCTTGAAAACTTCATGGTTAAATTTGATATTTTCAAAAAGAAAATGGAAGAGTGGATAAAAAGAGGAATTGATTTGATAATTTGACGTTTTTTAAAGAATGCTTAAATTATTAAAAAAAAAAAAAACCTAACTTTGCTATATTTTTTTTTAAAAAAAATATCCATATTCTCTTAAGAGTTATAATATATTACCTTTTATCTTTCATAAGGGTTTCAAAACTATTTTGAAGTAGAAATCTGTTGTTACCAAATTTTGGACAGGAGAATGCACCTGATCTACACGTGACAACAACGGTAAATTTGTAATTCTTGGGGGGAAGAACGCAGCCTGCAAGACAAGGAAAACCAGGCCACACCGACTCCGATGCTTAAGTCAGAGAGAGTTTCAAGAGAATTTTAGAGTTGTAGGGAGTTTTGATGGCATAAATCAACTGCTCTCCCATCCTGAGAGAGCTTAAATAACTCCTCCCTAGGGTTTTTCGTGGGTTTCCGTTGCTTAATGGGAAAAGGAAAAGTCGGAAGTGGGCTGGAACCAGACCTACAAATCAAATGGGACCGATGCAAGGGCCTGAAGTGAGCCTTGCATCTTGTCGGCCTCGGCCGAGGCCGACCACGGGCTTGGGCCGAGGCCGAGGCCGACCACGTGCTTGGGCCAAAGTCCAAGCACGCCCTCTCGTCCCTTTTCGCCTCGGGTCAGTCCCTGTTCGATTTCGGTTCATTTGGACTTCATGTTGGGCTTCTTTCCTCGTTCGGCCTTTCGTTGCAGCCCTTTAACCAGCCCGTTCATTGTTGATCCAATTTCACCCATAACAAAATCCATTAGATTGTTGGATGAAAAATCAATGTGACATTGATATGAAACGGCGTGATAGTGACTAGGCGCAAATTACTTTTGAGTTATGTACGATTCAAGTAGAATACAACTGAAATGTGCTTGTAACCTACCCTAACTGACACTTAAATTGTGAGACAAGTTCATCATCTATTTAATGCTTTTCTAAGTACTTTTAGG

mRNA sequence

CTCCCACATATAAATAAGGCAATAAAATATAAAATTCATAAACATGTGCCCGACCAATAAGCAATAATCGACCGTGTATTTGGTATAGGCACTAAAAAAATCCGAGAACGGCAGAACCCCAATCGCATCTTGCTGAAGAAAATGACGATTATACCCCTGCAACGCGCCTTCTTCGAAATGACATAGCCTGCAAATCCGAATCAGCGTAACCAATCTACATTATTATCTGTACCTTAGTTGGCTTCCATGGCCGCTCCTTCCCCGAAAACCCCATTGACCTTCTTCTTCTTCACTCTTCCCCCATATATATCCCCCATTCCACTCCCTCTTCTCACACTCAAACCAAAGCCCTAATCCCTAATTCCTAATTCCAATCCCATCTTACTTCTCGCTCCAAATTTCCAATGGCTTTTGCTGCAAGTGCAAACCAATCGGGATTCGAGGACTGGCTGCCGACGATGGCGCAGAGGCTGGGCGGCGAGGGTTTGATTGGGGAGCTTTGCAATGGGTTCAATCTTCTGATGGACAGAGAGAAGGGGGTGATCGATTTCCAGAGCTTGAAGAGGAACGCGGCGGCGCTAGGGCTGGGGGATCTGAGCGATGAGGATCTCAGAGCGATGTTGAGGGAGGGGGATTTCGACGGCGACGGCGCTCTCAATCAGATGGAGTTTTGTGTTCTTATGTTCAGATTGAGCCCTGAATTGATGGAAGAGTCCTGCTTCTTGCTCGAGGAGGCTCTAGGCCATGAGTTTAAGCATTTTTGTTGATTTCTTTTTCTTTTGTGTAATTAATTACTGATTTGATTTAGTGAATATACTTGGAAAACAAAATTAAAAGAACAAATTGTTACGATTTTTCTTTTTGCCACTTTCAATTTCATCCAATTAACATGGCAACTTCAGTTTTCACTAAACCTACTCCAAATTTTACAAATAAGTAAAAATTTTCTTTAAACAACTTAAGAACAACAAAATTAATCAACACAAGATCCACCTGAAATAAATCTTTCAAAATTAATATATTTCAATATAAATTCAACTCTAGGGCTATTTTTTAAACTATTGCACTGAACATATTTTATAATTACTGAATGCACTTAAGAATATTTTTCTTGAGGATTTTTCAAAATGCAATTATAACATTTTTTCAAATTTAAAGTTAAAGATGGTCAAATATTTGTTTCACATACACCTTGAAAACTTCATGGTTAAATTTGATATTTTCAAAAAGAAAATGGAAGAGTGGATAAAAAGAGGAATTGATTTGATAATTTGACGTTTTTTAAAGAATGCTTAAATTATTAAAAAAAAAAAAAACCTAACTTTGCTATATTTTTTTTTAAAAAAAATATCCATATTCTCTTAAGAGTTATAATATATTACCTTTTATCTTTCATAAGGGTTTCAAAACTATTTTGAAGTAGAAATCTGTTGTTACCAAATTTTGGACAGGAGAATGCACCTGATCTACACGTGACAACAACGGTAAATTTGTAATTCTTGGGGGGAAGAACGCAGCCTGCAAGACAAGGAAAACCAGGCCACACCGACTCCGATGCTTAAGTCAGAGAGAGTTTCAAGAGAATTTTAGAGTTGTAGGGAGTTTTGATGGCATAAATCAACTGCTCTCCCATCCTGAGAGAGCTTAAATAACTCCTCCCTAGGGTTTTTCGTGGGTTTCCGTTGCTTAATGGGAAAAGGAAAAGTCGGAAGTGGGCTGGAACCAGACCTACAAATCAAATGGGACCGATGCAAGGGCCTGAAGTGAGCCTTGCATCTTGTCGGCCTCGGCCGAGGCCGACCACGGGCTTGGGCCGAGGCCGAGGCCGACCACGTGCTTGGGCCAAAGTCCAAGCACGCCCTCTCGTCCCTTTTCGCCTCGGGTCAGTCCCTGTTCGATTTCGGTTCATTTGGACTTCATGTTGGGCTTCTTTCCTCGTTCGGCCTTTCGTTGCAGCCCTTTAACCAGCCCGTTCATTGTTGATCCAATTTCACCCATAACAAAATCCATTAGATTGTTGGATGAAAAATCAATGTGACATTGATATGAAACGGCGTGATAGTGACTAGGCGCAAATTACTTTTGAGTTATGTACGATTCAAGTAGAATACAACTGAAATGTGCTTGTAACCTACCCTAACTGACACTTAAATTGTGAGACAAGTTCATCATCTATTTAATGCTTTTCTAAGTACTTTTAGG

Coding sequence (CDS)

ATGGCTTTTGCTGCAAGTGCAAACCAATCGGGATTCGAGGACTGGCTGCCGACGATGGCGCAGAGGCTGGGCGGCGAGGGTTTGATTGGGGAGCTTTGCAATGGGTTCAATCTTCTGATGGACAGAGAGAAGGGGGTGATCGATTTCCAGAGCTTGAAGAGGAACGCGGCGGCGCTAGGGCTGGGGGATCTGAGCGATGAGGATCTCAGAGCGATGTTGAGGGAGGGGGATTTCGACGGCGACGGCGCTCTCAATCAGATGGAGTTTTGTGTTCTTATGTTCAGATTGAGCCCTGAATTGATGGAAGAGTCCTGCTTCTTGCTCGAGGAGGCTCTAGGCCATGAGTTTAAGCATTTTTGTTGA

Protein sequence

MAFAASANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGDLSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC

Homology

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BLAST of Tan0013363 vs. ExPASy Swiss-Prot
Match: O81831 (Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.3e-39
Identity = 82/109 (75.23%), Postives = 92/109 (84.40%), Query Frame = 0


Query: 9   QSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAA-LGLGDLSDE 68
           Q  F D+LPTMA  LGGEGLIGELCNGF LLMDREKGVI F+SL+RNAAA LGLGDL+DE
Sbjct: 16  QPNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDE 75

Query: 69  DLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEF 117
           D+R M++EGDFD DGALNQMEFCVLMFRLSP+LME S  L+ E +  EF
Sbjct: 76  DVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

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BLAST of Tan0013363 vs. ExPASy Swiss-Prot
Match: Q9LSQ6 (Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 3.3e-35
Identity = 72/106 (67.92%), Postives = 88/106 (83.02%), Query Frame = 0


Query: 12  FEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAA-ALGLGDLSDEDLR 71
           F+D+ PTMA +LGGEGLI E+C GF LLMD++KGVI F+SL+RNA+  LGLGDL+D+D+R
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 72  AMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEF 117
            M+ EGDFD DGALNQMEFCVLMFRLSPELME S  ++ E +  EF
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

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BLAST of Tan0013363 vs. ExPASy Swiss-Prot
Match: Q9ZPX9 (Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2)

HSP 1 Score: 118.6 bits (296), Expect = 4.8e-26
Identity = 57/107 (53.27%), Postives = 78/107 (72.90%), Query Frame = 0


Query: 9   QSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGDLSDED 68
           ++ +ED LP MA+++  E  + ELC GF+LL D E+ +I  +SL+RN+  LG+  +S ED
Sbjct: 19  ETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKED 78

Query: 69  LRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHE 116
            + M+REGD DGDGALNQ EFCVLM RLSPE+ME++   LE+AL  E
Sbjct: 79  AQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

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BLAST of Tan0013363 vs. ExPASy Swiss-Prot
Match: Q2TBN3 (Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.3e-10
Identity = 36/86 (41.86%), Postives = 51/86 (59.30%), Query Frame = 0


Query: 12  FEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGDLSDEDLRA 71
           F D+L  M Q++  +    E+   F L  D E G I F++LKR A  LG  +LSDE+L+ 
Sbjct: 86  FSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLSDEELQE 145

Query: 72  MLREGDFDGDGALNQMEFCVLMFRLS 98
           M+ E D DGDG +N+ EF  +M + S
Sbjct: 146 MIDEADRDGDGEVNEQEFLRIMKKTS 170

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BLAST of Tan0013363 vs. ExPASy Swiss-Prot
Match: Q9R1K9 (Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.4e-09
Identity = 35/86 (40.70%), Postives = 51/86 (59.30%), Query Frame = 0


Query: 12  FEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGDLSDEDLRA 71
           F D+L  M Q++  +    E+   F L  D E G I F++LKR A  LG  +L+DE+L+ 
Sbjct: 86  FSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLTDEELQE 145

Query: 72  MLREGDFDGDGALNQMEFCVLMFRLS 98
           M+ E D DGDG +N+ EF  +M + S
Sbjct: 146 MIDEADRDGDGEVNEQEFLRIMKKTS 170

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BLAST of Tan0013363 vs. NCBI nr
Match: XP_004149304.1 (calcium-binding protein KRP1 [Cucumis sativus] >KGN56198.1 hypothetical protein Csa_010363 [Cucumis sativus])

HSP 1 Score: 216.1 bits (549), Expect = 1.7e-52
Identity = 106/117 (90.60%), Postives = 111/117 (94.87%), Query Frame = 0


Query: 4   AASANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGD 63
           A SA +SGFEDWLPTMA+RLGGEGLIGELCNGFNLLM+REKGVIDF+SLKRNAAALGLGD
Sbjct: 2   AFSAEKSGFEDWLPTMAERLGGEGLIGELCNGFNLLMNREKGVIDFESLKRNAAALGLGD 61

Query: 64  LSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC 121
           LSDEDLR+MLREGDFDGDGALNQMEFCVLMFRLSPELMEES FLLEEAL  EFK FC
Sbjct: 62  LSDEDLRSMLREGDFDGDGALNQMEFCVLMFRLSPELMEESWFLLEEALHQEFKDFC 118

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BLAST of Tan0013363 vs. NCBI nr
Match: XP_008452312.1 (PREDICTED: calcium-binding protein PBP1 [Cucumis melo])

HSP 1 Score: 214.5 bits (545), Expect = 4.9e-52
Identity = 105/117 (89.74%), Postives = 110/117 (94.02%), Query Frame = 0


Query: 4   AASANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGD 63
           A SA +SGFEDWLPTMA+RLGGEGLIGELCNGFNLLMDREKGVIDF+SLKRNAAALGL D
Sbjct: 2   AFSAEKSGFEDWLPTMAERLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLAD 61

Query: 64  LSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC 121
           LSDEDLR+MLREGDFDGDGALNQMEFCVLMFRLSPELM+ES FLLEEAL  EFK FC
Sbjct: 62  LSDEDLRSMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALHQEFKDFC 118

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BLAST of Tan0013363 vs. NCBI nr
Match: XP_038903612.1 (calcium-binding protein KRP1-like [Benincasa hispida])

HSP 1 Score: 214.2 bits (544), Expect = 6.4e-52
Identity = 104/117 (88.89%), Postives = 109/117 (93.16%), Query Frame = 0


Query: 4   AASANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGD 63
           A  A QSGF+DWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDF+SLKRNAAALGL D
Sbjct: 30  AFGAEQSGFQDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLAD 89

Query: 64  LSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC 121
           LSDEDLR+MLREGDFDGDGALNQMEFCVLMFRLSP+LM+ES FLLEEAL  EFK FC
Sbjct: 90  LSDEDLRSMLREGDFDGDGALNQMEFCVLMFRLSPDLMDESWFLLEEALNQEFKDFC 146

[Close]

BLAST of Tan0013363 vs. NCBI nr
Match: XP_022929391.1 (calcium-binding protein PBP1-like [Cucurbita moschata] >XP_023552392.1 calcium-binding protein PBP1 [Cucurbita pepo subsp. pepo] >KAG6577399.1 Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015477.1 Calcium-binding protein PBP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 208.8 bits (530), Expect = 2.7e-50
Identity = 104/120 (86.67%), Postives = 109/120 (90.83%), Query Frame = 0


Query: 1   MAFAASANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALG 60
           MAF   A+QSGFEDWLP MA+RLGGEGLIGELCNGFNLL DREKGVIDF+SLKRNAAALG
Sbjct: 1   MAFV--ADQSGFEDWLPKMAERLGGEGLIGELCNGFNLLKDREKGVIDFESLKRNAAALG 60

Query: 61  LGDLSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC 120
           L DLSDEDLR MLREGDFDGDGALNQMEFCVLMFRLSPELM+ES FLLE+AL  EFK FC
Sbjct: 61  LADLSDEDLREMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEDALNQEFKGFC 118

[Close]

BLAST of Tan0013363 vs. NCBI nr
Match: XP_022142860.1 (calcium-binding protein PBP1 [Momordica charantia])

HSP 1 Score: 205.7 bits (522), Expect = 2.3e-49
Identity = 101/120 (84.17%), Postives = 109/120 (90.83%), Query Frame = 0


Query: 4   AASANQ---SGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALG 63
           AA ANQ   SGF DWLPTMA RLGGEGLIGELC+GF+LLMDREKGVIDF+SLKRNAAALG
Sbjct: 3   AAGANQVPESGFHDWLPTMAARLGGEGLIGELCSGFSLLMDREKGVIDFESLKRNAAALG 62

Query: 64  LGDLSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC 121
           LGDL+DEDLR MLREGDFDGDGALNQMEFCVLMFRLSPELM+ES F L++AL HEF+ FC
Sbjct: 63  LGDLTDEDLRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDESLFCLDQALTHEFRDFC 122

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BLAST of Tan0013363 vs. ExPASy TrEMBL
Match: A0A0A0L347 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G099590 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 8.1e-53
Identity = 106/117 (90.60%), Postives = 111/117 (94.87%), Query Frame = 0


Query: 4   AASANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGD 63
           A SA +SGFEDWLPTMA+RLGGEGLIGELCNGFNLLM+REKGVIDF+SLKRNAAALGLGD
Sbjct: 2   AFSAEKSGFEDWLPTMAERLGGEGLIGELCNGFNLLMNREKGVIDFESLKRNAAALGLGD 61

Query: 64  LSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC 121
           LSDEDLR+MLREGDFDGDGALNQMEFCVLMFRLSPELMEES FLLEEAL  EFK FC
Sbjct: 62  LSDEDLRSMLREGDFDGDGALNQMEFCVLMFRLSPELMEESWFLLEEALHQEFKDFC 118

[Close]

BLAST of Tan0013363 vs. ExPASy TrEMBL
Match: A0A1S3BSY1 (calcium-binding protein PBP1 OS=Cucumis melo OX=3656 GN=LOC103493375 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.4e-52
Identity = 105/117 (89.74%), Postives = 110/117 (94.02%), Query Frame = 0


Query: 4   AASANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGD 63
           A SA +SGFEDWLPTMA+RLGGEGLIGELCNGFNLLMDREKGVIDF+SLKRNAAALGL D
Sbjct: 2   AFSAEKSGFEDWLPTMAERLGGEGLIGELCNGFNLLMDREKGVIDFESLKRNAAALGLAD 61

Query: 64  LSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC 121
           LSDEDLR+MLREGDFDGDGALNQMEFCVLMFRLSPELM+ES FLLEEAL  EFK FC
Sbjct: 62  LSDEDLRSMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEEALHQEFKDFC 118

[Close]

BLAST of Tan0013363 vs. ExPASy TrEMBL
Match: A0A6J1EN05 (calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111435976 PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.3e-50
Identity = 104/120 (86.67%), Postives = 109/120 (90.83%), Query Frame = 0


Query: 1   MAFAASANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALG 60
           MAF   A+QSGFEDWLP MA+RLGGEGLIGELCNGFNLL DREKGVIDF+SLKRNAAALG
Sbjct: 1   MAFV--ADQSGFEDWLPKMAERLGGEGLIGELCNGFNLLKDREKGVIDFESLKRNAAALG 60

Query: 61  LGDLSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC 120
           L DLSDEDLR MLREGDFDGDGALNQMEFCVLMFRLSPELM+ES FLLE+AL  EFK FC
Sbjct: 61  LADLSDEDLREMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEDALNQEFKGFC 118

[Close]

BLAST of Tan0013363 vs. ExPASy TrEMBL
Match: A0A6J1CM41 (calcium-binding protein PBP1 OS=Momordica charantia OX=3673 GN=LOC111012869 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-49
Identity = 101/120 (84.17%), Postives = 109/120 (90.83%), Query Frame = 0


Query: 4   AASANQ---SGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALG 63
           AA ANQ   SGF DWLPTMA RLGGEGLIGELC+GF+LLMDREKGVIDF+SLKRNAAALG
Sbjct: 3   AAGANQVPESGFHDWLPTMAARLGGEGLIGELCSGFSLLMDREKGVIDFESLKRNAAALG 62

Query: 64  LGDLSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHFC 121
           LGDL+DEDLR MLREGDFDGDGALNQMEFCVLMFRLSPELM+ES F L++AL HEF+ FC
Sbjct: 63  LGDLTDEDLRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDESLFCLDQALTHEFRDFC 122

[Close]

BLAST of Tan0013363 vs. ExPASy TrEMBL
Match: A0A6J1JAW3 (calcium-binding protein PBP1 OS=Cucurbita maxima OX=3661 GN=LOC111483314 PE=4 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 2.4e-49
Identity = 103/119 (86.55%), Postives = 107/119 (89.92%), Query Frame = 0


Query: 1   MAFAASANQSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALG 60
           MAF   A+QSGFEDWLP MA+RLGGEGLIGELCNGFNLL DREKGVIDF SLKRNAAALG
Sbjct: 1   MAFV--ADQSGFEDWLPKMAERLGGEGLIGELCNGFNLLKDREKGVIDFSSLKRNAAALG 60

Query: 61  LGDLSDEDLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEFKHF 120
           L DLSDEDLR MLREGDFDGDGALNQMEFCVLMFRLSPELM+ES FLLE+AL  EFK F
Sbjct: 61  LADLSDEDLREMLREGDFDGDGALNQMEFCVLMFRLSPELMDESWFLLEDALSQEFKGF 117

[Close]

BLAST of Tan0013363 vs. TAIR 10
Match: AT4G27280.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 163.7 bits (413), Expect = 9.2e-41
Identity = 82/109 (75.23%), Postives = 92/109 (84.40%), Query Frame = 0


Query: 9   QSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAA-LGLGDLSDE 68
           Q  F D+LPTMA  LGGEGLIGELCNGF LLMDREKGVI F+SL+RNAAA LGLGDL+DE
Sbjct: 16  QPNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDE 75

Query: 69  DLRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEF 117
           D+R M++EGDFD DGALNQMEFCVLMFRLSP+LME S  L+ E +  EF
Sbjct: 76  DVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

[Close]

BLAST of Tan0013363 vs. TAIR 10
Match: AT5G54490.1 (pinoid-binding protein 1 )

HSP 1 Score: 149.1 bits (375), Expect = 2.3e-36
Identity = 72/106 (67.92%), Postives = 88/106 (83.02%), Query Frame = 0


Query: 12  FEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAA-ALGLGDLSDEDLR 71
           F+D+ PTMA +LGGEGLI E+C GF LLMD++KGVI F+SL+RNA+  LGLGDL+D+D+R
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 72  AMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHEF 117
            M+ EGDFD DGALNQMEFCVLMFRLSPELME S  ++ E +  EF
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

[Close]

BLAST of Tan0013363 vs. TAIR 10
Match: AT2G46600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 118.6 bits (296), Expect = 3.4e-27
Identity = 57/107 (53.27%), Postives = 78/107 (72.90%), Query Frame = 0


Query: 9   QSGFEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGDLSDED 68
           ++ +ED LP MA+++  E  + ELC GF+LL D E+ +I  +SL+RN+  LG+  +S ED
Sbjct: 19  ETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKED 78

Query: 69  LRAMLREGDFDGDGALNQMEFCVLMFRLSPELMEESCFLLEEALGHE 116
            + M+REGD DGDGALNQ EFCVLM RLSPE+ME++   LE+AL  E
Sbjct: 79  AQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

[Close]

BLAST of Tan0013363 vs. TAIR 10
Match: AT4G37010.1 (centrin 2 )

HSP 1 Score: 48.5 bits (114), Expect = 4.3e-06
Identity = 31/93 (33.33%), Postives = 46/93 (49.46%), Query Frame = 0


Query: 8   NQSG---FEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGDL 67
           NQSG   F++++  M  + G    I EL   F ++     G I  + +K  A  LG  + 
Sbjct: 74  NQSGAIDFDEFVHMMTTKFGERDSIDELSKAFKIIDHDNNGKISPRDIKMIAKELG-ENF 133

Query: 68  SDEDLRAMLREGDFDGDGALNQMEFCVLMFRLS 98
           +D D+  M+ E D D DG +N  EF  +M R S
Sbjct: 134 TDNDIEEMIEEADRDKDGEVNLEEFMKMMKRTS 165

[Close]

BLAST of Tan0013363 vs. TAIR 10
Match: AT4G37010.2 (centrin 2 )

HSP 1 Score: 48.5 bits (114), Expect = 4.3e-06
Identity = 31/93 (33.33%), Postives = 46/93 (49.46%), Query Frame = 0


Query: 8   NQSG---FEDWLPTMAQRLGGEGLIGELCNGFNLLMDREKGVIDFQSLKRNAAALGLGDL 67
           NQSG   F++++  M  + G    I EL   F ++     G I  + +K  A  LG  + 
Sbjct: 78  NQSGAIDFDEFVHMMTTKFGERDSIDELSKAFKIIDHDNNGKISPRDIKMIAKELG-ENF 137

Query: 68  SDEDLRAMLREGDFDGDGALNQMEFCVLMFRLS 98
           +D D+  M+ E D D DG +N  EF  +M R S
Sbjct: 138 TDNDIEEMIEEADRDKDGEVNLEEFMKMMKRTS 169

The following BLAST results are available for this feature:

BLAST of Tan0013363 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81831	1.3e-39	75.23	Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1	[more]
Q9LSQ6	3.3e-35	67.92	Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1	[more]
Q9ZPX9	4.8e-26	53.27	Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2	[more]
Q2TBN3	6.3e-10	41.86	Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1	[more]
Q9R1K9	1.4e-09	40.70	Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1	[more]

BLAST of Tan0013363 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149304.1	1.7e-52	90.60	calcium-binding protein KRP1 [Cucumis sativus] >KGN56198.1 hypothetical protein ...	[more]
XP_008452312.1	4.9e-52	89.74	PREDICTED: calcium-binding protein PBP1 [Cucumis melo]	[more]
XP_038903612.1	6.4e-52	88.89	calcium-binding protein KRP1-like [Benincasa hispida]	[more]
XP_022929391.1	2.7e-50	86.67	calcium-binding protein PBP1-like [Cucurbita moschata] >XP_023552392.1 calcium-b...	[more]
XP_022142860.1	2.3e-49	84.17	calcium-binding protein PBP1 [Momordica charantia]	[more]

BLAST of Tan0013363 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L347	8.1e-53	90.60	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G099590 PE=...	[more]
A0A1S3BSY1	2.4e-52	89.74	calcium-binding protein PBP1 OS=Cucumis melo OX=3656 GN=LOC103493375 PE=4 SV=1	[more]
A0A6J1EN05	1.3e-50	86.67	calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111435976 ...	[more]
A0A6J1CM41	1.1e-49	84.17	calcium-binding protein PBP1 OS=Momordica charantia OX=3673 GN=LOC111012869 PE=4...	[more]
A0A6J1JAW3	2.4e-49	86.55	calcium-binding protein PBP1 OS=Cucurbita maxima OX=3661 GN=LOC111483314 PE=4 SV...	[more]

BLAST of Tan0013363 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27280.1	9.2e-41	75.23	Calcium-binding EF-hand family protein	[more]
AT5G54490.1	2.3e-36	67.92	pinoid-binding protein 1	[more]
AT2G46600.1	3.4e-27	53.27	Calcium-binding EF-hand family protein	[more]
AT4G37010.1	4.3e-06	33.33	centrin 2	[more]
AT4G37010.2	4.3e-06	33.33	centrin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 68..96 e-value: 0.008 score: 25.4
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 43..95 e-value: 8.5E-20 score: 70.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 64..99 score: 10.998585
None	No IPR available	GENE3D	1.10.238.10		coord: 10..119 e-value: 1.7E-20 score: 75.0
None	No IPR available	PANTHER	PTHR47319:SF6	CALCIUM-BINDING PROTEIN KRP1	coord: 6..119
IPR044205	Calcium-binding protein KIC/PBP1/KRP1	PANTHER	PTHR47319	CALCIUM-BINDING PROTEIN KIC	coord: 6..119
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 77..89
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 10..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0013363.1	Tan0013363.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding