Tan0013286 (gene) Snake gourd v1

Overview
NameTan0013286
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
LocationLG01: 18568327 .. 18570079 (+)
RNA-Seq ExpressionTan0013286
SyntenyTan0013286
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAAATCAGAGTTTGGAGAAAACCCATAAGAAACCTCTTTCTTTCCTGAGCTTTAATTTGTGTCTCATAATAATCATCCATGGGCGTCTTAACTTTCCTGCTCTTCCTCCTTTCTGCCACTGCTCTCGCAGAAGCCGGATCTGGATGGGTTGGAGTTAACTACGGCCGAATCGCCGACAACCTTCCCCCGCCGGAAAAAGTGGTTGAGCTCCTCAAACAGCAAGGAATCGACCGAATCAAGCTCTTCGACAGCGACGCCACCGTCCTCACCGCCCTCTCCAACTCCAACATCTCCGTCGTTGTCGCCCTTCCCAACGAGCTCCTATCCTCTGCCGCTGCTGATTCTTCCTTCACCGACAATTGGGTCCAATCCAAAATCTCCCATTTCTACCCCGCCACCAAAATCGACGCCATCGCCGTCGGCAACGAGGTCTTCGTCGACCCCAACAACACAACTAAATTCCTTGTCCCCGCCATGAAAAACGTCTACTCCTCCCTCGAAAAATACAACCTCCACAACTCCATCAAAATTTCATCCCCCATTGCCCTCTCTGCCCTCGGTTCCTCCTACCCGACCTCCTCCGGTTCGTTCAAACCAGACCTCGTCGAGCCAGTCATCAAGCCCATGCTCGACTTCCTCCGCCAGACCCAATCCTATCTAATGGTTAACGCTTACCCCTTCTTCGCCTACGCCGCTAACTCCGACCAGATCTCTTTGGACTACGCTCTCTTTCGGGAGAATCCCGGCGTTGTGGACTCCGGCAATGGGCTGAAATACTCTAGCCTTTTCGAGGCTCAAATCGACGCCGTTTACGCCGCCATGTCGGCGCTCAAATACGACGACGTTAAGGTCGTTGTTACCGAGACTGGGTGGCCGTCGAAAGGCGACGAGAAGGAATTGGGAGCGAGTGGGGAGAATGCGGCGTCGTATAACGGCAATTTGGTGAAGAGAGTTCTGACCGGAAGTGGGACCCCCTTGAGACCGCAGGACCCATTAAACGTCTTTCTCTTCGCGTTGTTCAATGAGAATCAGAAGTCCGGGCCCACCTCCGAGAGGAATTACGGCTTGTTTTACCCCGACGAGAAGAAGGTTTATGAGATTCCTCTCACACGCGCCGACTTGGAGAGTGGAGCTCCGACGCCGGTGAATGAGAGTAAGGCTCAGGTGCCGACCGGCGACTTCTCGCCGTTGTCGAAAGGGCAGACGTGGTGTGTGGCCAATGGGCACGCGGCGGCGGAGAAGCTGCAGGCGGCGTTGGATTATGCCTGTGGAGAGGGGGGCGCTGATTGCCGTCCGATCCAGCCGGGTTCCACGTGTTACAATCCTAACTCCCTCGAGGCACACGCTTCGTACGCTTTCAATAGTTATTACCAGAAGATGGCTCGTGCCAGTGGCACGTGCTACTTCGGTGGTGCTGCTTACGTCGTCACTCAACCTCCCAGTAAGTATCGAATTGCAAGTTCAATAATCTCTAATTTTAATTCATTTATTATTTTTTGCTTTTAGAATAATTAACGGTGTCGTTTTTGTGGTGCAGAATACGGAAAATGCGAGTTCCCCACTGGGTACTGAGTTCGGACAGGCACCTATAGAAATTGATCGACCACAATCTAATTTCATTTGGTGACATAGTTGACTTCTTCTTTCGTGTTTTTTTTTTTTTTGGATTCTAATATATTATTTGTGTGTAATAGTTAGATATTTACTTATAAAAGTTATACTTGGTGGGGCTATTGCTTCCCAATTTTTAA

mRNA sequence

ATAAAATCAGAGTTTGGAGAAAACCCATAAGAAACCTCTTTCTTTCCTGAGCTTTAATTTGTGTCTCATAATAATCATCCATGGGCGTCTTAACTTTCCTGCTCTTCCTCCTTTCTGCCACTGCTCTCGCAGAAGCCGGATCTGGATGGGTTGGAGTTAACTACGGCCGAATCGCCGACAACCTTCCCCCGCCGGAAAAAGTGGTTGAGCTCCTCAAACAGCAAGGAATCGACCGAATCAAGCTCTTCGACAGCGACGCCACCGTCCTCACCGCCCTCTCCAACTCCAACATCTCCGTCGTTGTCGCCCTTCCCAACGAGCTCCTATCCTCTGCCGCTGCTGATTCTTCCTTCACCGACAATTGGGTCCAATCCAAAATCTCCCATTTCTACCCCGCCACCAAAATCGACGCCATCGCCGTCGGCAACGAGGTCTTCGTCGACCCCAACAACACAACTAAATTCCTTGTCCCCGCCATGAAAAACGTCTACTCCTCCCTCGAAAAATACAACCTCCACAACTCCATCAAAATTTCATCCCCCATTGCCCTCTCTGCCCTCGGTTCCTCCTACCCGACCTCCTCCGGTTCGTTCAAACCAGACCTCGTCGAGCCAGTCATCAAGCCCATGCTCGACTTCCTCCGCCAGACCCAATCCTATCTAATGGTTAACGCTTACCCCTTCTTCGCCTACGCCGCTAACTCCGACCAGATCTCTTTGGACTACGCTCTCTTTCGGGAGAATCCCGGCGTTGTGGACTCCGGCAATGGGCTGAAATACTCTAGCCTTTTCGAGGCTCAAATCGACGCCGTTTACGCCGCCATGTCGGCGCTCAAATACGACGACGTTAAGGTCGTTGTTACCGAGACTGGGTGGCCGTCGAAAGGCGACGAGAAGGAATTGGGAGCGAGTGGGGAGAATGCGGCGTCGTATAACGGCAATTTGGTGAAGAGAGTTCTGACCGGAAGTGGGACCCCCTTGAGACCGCAGGACCCATTAAACGTCTTTCTCTTCGCGTTGTTCAATGAGAATCAGAAGTCCGGGCCCACCTCCGAGAGGAATTACGGCTTGTTTTACCCCGACGAGAAGAAGGTTTATGAGATTCCTCTCACACGCGCCGACTTGGAGAGTGGAGCTCCGACGCCGGTGAATGAGAGTAAGGCTCAGGTGCCGACCGGCGACTTCTCGCCGTTGTCGAAAGGGCAGACGTGGTGTGTGGCCAATGGGCACGCGGCGGCGGAGAAGCTGCAGGCGGCGTTGGATTATGCCTGTGGAGAGGGGGGCGCTGATTGCCGTCCGATCCAGCCGGGTTCCACGTGTTACAATCCTAACTCCCTCGAGGCACACGCTTCGTACGCTTTCAATAGTTATTACCAGAAGATGGCTCGTGCCAGTGGCACGTGCTACTTCGGTGGTGCTGCTTACGTCGTCACTCAACCTCCCAAATACGGAAAATGCGAGTTCCCCACTGGGTACTGAGTTCGGACAGGCACCTATAGAAATTGATCGACCACAATCTAATTTCATTTGGTGACATAGTTGACTTCTTCTTTCGTGTTTTTTTTTTTTTTGGATTCTAATATATTATTTGTGTGTAATAGTTAGATATTTACTTATAAAAGTTATACTTGGTGGGGCTATTGCTTCCCAATTTTTAA

Coding sequence (CDS)

ATGGGCGTCTTAACTTTCCTGCTCTTCCTCCTTTCTGCCACTGCTCTCGCAGAAGCCGGATCTGGATGGGTTGGAGTTAACTACGGCCGAATCGCCGACAACCTTCCCCCGCCGGAAAAAGTGGTTGAGCTCCTCAAACAGCAAGGAATCGACCGAATCAAGCTCTTCGACAGCGACGCCACCGTCCTCACCGCCCTCTCCAACTCCAACATCTCCGTCGTTGTCGCCCTTCCCAACGAGCTCCTATCCTCTGCCGCTGCTGATTCTTCCTTCACCGACAATTGGGTCCAATCCAAAATCTCCCATTTCTACCCCGCCACCAAAATCGACGCCATCGCCGTCGGCAACGAGGTCTTCGTCGACCCCAACAACACAACTAAATTCCTTGTCCCCGCCATGAAAAACGTCTACTCCTCCCTCGAAAAATACAACCTCCACAACTCCATCAAAATTTCATCCCCCATTGCCCTCTCTGCCCTCGGTTCCTCCTACCCGACCTCCTCCGGTTCGTTCAAACCAGACCTCGTCGAGCCAGTCATCAAGCCCATGCTCGACTTCCTCCGCCAGACCCAATCCTATCTAATGGTTAACGCTTACCCCTTCTTCGCCTACGCCGCTAACTCCGACCAGATCTCTTTGGACTACGCTCTCTTTCGGGAGAATCCCGGCGTTGTGGACTCCGGCAATGGGCTGAAATACTCTAGCCTTTTCGAGGCTCAAATCGACGCCGTTTACGCCGCCATGTCGGCGCTCAAATACGACGACGTTAAGGTCGTTGTTACCGAGACTGGGTGGCCGTCGAAAGGCGACGAGAAGGAATTGGGAGCGAGTGGGGAGAATGCGGCGTCGTATAACGGCAATTTGGTGAAGAGAGTTCTGACCGGAAGTGGGACCCCCTTGAGACCGCAGGACCCATTAAACGTCTTTCTCTTCGCGTTGTTCAATGAGAATCAGAAGTCCGGGCCCACCTCCGAGAGGAATTACGGCTTGTTTTACCCCGACGAGAAGAAGGTTTATGAGATTCCTCTCACACGCGCCGACTTGGAGAGTGGAGCTCCGACGCCGGTGAATGAGAGTAAGGCTCAGGTGCCGACCGGCGACTTCTCGCCGTTGTCGAAAGGGCAGACGTGGTGTGTGGCCAATGGGCACGCGGCGGCGGAGAAGCTGCAGGCGGCGTTGGATTATGCCTGTGGAGAGGGGGGCGCTGATTGCCGTCCGATCCAGCCGGGTTCCACGTGTTACAATCCTAACTCCCTCGAGGCACACGCTTCGTACGCTTTCAATAGTTATTACCAGAAGATGGCTCGTGCCAGTGGCACGTGCTACTTCGGTGGTGCTGCTTACGTCGTCACTCAACCTCCCAAATACGGAAAATGCGAGTTCCCCACTGGGTACTGA

Protein sequence

MGVLTFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLTALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNNTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPMLDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAVYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQDPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVNESKAQVPTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHASYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTGY
Homology
BLAST of Tan0013286 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.2e-101
Identity = 193/476 (40.55%), Postives = 296/476 (62.18%), Query Frame = 0

Query: 1   MGVLTFLLFLLSATALAEAGSGW---VGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFD 60
           MG    L+F +  + LA    G    +G+ YGR ADNLP P +V EL++   I  ++++D
Sbjct: 1   MGQRLNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYD 60

Query: 61  SDATVLTALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNE 120
           ++  VL A +N+ I +++ +PN  L + A   S  D W+ + I  +YP+TKI +I+VG E
Sbjct: 61  ANIDVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLE 120

Query: 121 VFVDPNNTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVE 180
           V   P+N T  ++PAM+N++++L+K  L   IKISS  +L+ L  S+P SS SF      
Sbjct: 121 VTEAPDNATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HS 180

Query: 181 PVIKPMLDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLF 240
             +KPML+FL + +S  M++ YP++AY  +++++ L+YALF  +  VVD   GL YS++F
Sbjct: 181 AFLKPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMF 240

Query: 241 EAQIDAVYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSG 300
           +AQ+DA+Y A++A+ +  VKV+VTE+GWPSKG  KE  A+ ENA +YN NL++ V+   G
Sbjct: 241 DAQLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPG 300

Query: 301 TPLRPQDPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN 360
           TP +P + ++V+LF+LFNEN+K G  SERN+G+FY +   VY +  T  +    +PT  N
Sbjct: 301 TPAKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPT--N 360

Query: 361 ESKAQVPTGDFSPLSKGQT--------------WCVANGHAAAEKLQAALDYACGEGGAD 420
            +    P+   SP+  G +              WC+A+  A+  +LQ ALD+ACG G  D
Sbjct: 361 STTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVD 420

Query: 421 CRPIQPGSTCYNPNSLEAHASYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKC 460
           C  +QP   C+ P+++ +HASYAFN+YYQ+   +S  C F GA+  V + P YG C
Sbjct: 421 CSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNC 473

BLAST of Tan0013286 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 4.6e-101
Identity = 182/444 (40.99%), Postives = 282/444 (63.51%), Query Frame = 0

Query: 22  GWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLTALSNSNISVVVALPNEL 81
           G VGV YGR AD+LP P KVV+L++Q  I  ++++D ++ VL A  N++I +++ +PN  
Sbjct: 24  GKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSD 83

Query: 82  LSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPN-NTTKFLVPAMKNVYSSL 141
           L++ +   S  D W+++ +  +YP TKI  I VG E   DP+ N + F+VPAM+NV ++L
Sbjct: 84  LNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTAL 143

Query: 142 EKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPMLDFLRQTQSYLMVNAYP 201
            K  L   IK+S+ ++L  L  S+P S+G+F        ++PML+FL + +S  M++ YP
Sbjct: 144 RKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAY-FLRPMLEFLAENKSPFMIDLYP 203

Query: 202 FFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAVYAAMSALKYDDVKVVV 261
           ++AY  + + +SLDY LF  +  V+D   GL Y ++F+AQ+DA+Y A++AL +  +K++V
Sbjct: 204 YYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMV 263

Query: 262 TETGWPSKGDEKE-LGASGENAASYNGNLVKRVLTGSGTPLRPQDPLNVFLFALFNENQK 321
           TETGWP+KG  KE   AS +NA +YN N+++ V+T  GTP +P + +NV++F+LFNEN+K
Sbjct: 264 TETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENRK 323

Query: 322 SGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVNESKAQVPTGDFSPLSKGQTWCV 381
           +G  SERN+GLFYPD+  VY++  T      G     + + +   +   S      +WC+
Sbjct: 324 AGLDSERNWGLFYPDQTSVYQLDFT------GKSNGFHSNSSGTNSSGSS-----NSWCI 383

Query: 382 ANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHASYAFNSYYQKMARASG 441
           A+  A+   L+ ALD+ACG G  DC  IQP   C+ P++L +HAS+ FNSY+Q+      
Sbjct: 384 ASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDV 443

Query: 442 TCYFGGAAYVVTQPPKYGKCEFPT 464
            C FGGA   V + P Y KC + T
Sbjct: 444 ACSFGGAGVKVNKDPSYDKCIYIT 455

BLAST of Tan0013286 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 357.8 bits (917), Expect = 1.8e-97
Identity = 192/461 (41.65%), Postives = 287/461 (62.26%), Query Frame = 0

Query: 6   FLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLTA 65
           FLL  LS    + A   ++GVNYG++ADNLPPP + V+LL+   I +++L+ +D  ++ A
Sbjct: 9   FLLIFLSHFPSSHA-EPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKA 68

Query: 66  LSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFV--DPN 125
           L+ + + +V+   N  + S A+D +    W+ S +  FYPA+KI  I VGNE+ +  DPN
Sbjct: 69  LAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPN 128

Query: 126 NTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPM 185
              + L+PAM+NV  +LE  +L   IK+S+  +++ LGSS P SSGSF     +  +K +
Sbjct: 129 LVNQ-LLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAG-YQTGLKGI 188

Query: 186 LDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDA 245
           L FL  T S   +N YPFFAY ++    +L + LF  N G VDS  G+KY+++F+AQ+DA
Sbjct: 189 LQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDA 248

Query: 246 VYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQ 305
           V++A+ ++ ++ V++VV ETGW S+GD  E+GAS +NA +YNGNL+  + +  GTPL P 
Sbjct: 249 VHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPG 308

Query: 306 DPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVNESKAQV 365
            P++ ++FAL++EN K GP+SER +GLF  D   VY++ L +           + S +Q 
Sbjct: 309 KPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAK-----------SSSSSQT 368

Query: 366 PTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHA 425
           P+G  +  S G  WCV    A  E+LQA+LD+ACG  G DC  IQPG  C+ PN++ +HA
Sbjct: 369 PSGKVT--SSG--WCVPKKGATNEELQASLDWACGH-GIDCGAIQPGGACFEPNNVVSHA 428

Query: 426 SYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTG 465
           +YA N Y+QK  +    C F   A V +Q P Y  C +P G
Sbjct: 429 AYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

BLAST of Tan0013286 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 342.4 bits (877), Expect = 7.9e-93
Identity = 182/463 (39.31%), Postives = 276/463 (59.61%), Query Frame = 0

Query: 3   VLTFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATV 62
           +L   LFL +++AL++     +GVN G    N+P P +VV LLK Q I+R++L+D+D ++
Sbjct: 4   LLLLFLFLFASSALSQ--DSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSM 63

Query: 63  LTALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDP 122
           L A +++ + V++++PN+ L   +  ++   NWV   ++ +YPAT I  IAVG+EV    
Sbjct: 64  LLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSL 123

Query: 123 NNTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKP 182
            N    LV A+K + ++L   NL   IK+S+P + + +  S+P S   F     +PVI P
Sbjct: 124 TNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTW-DPVIVP 183

Query: 183 MLDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALF---RENPGVVDSGNGLKYSSLFEA 242
           +L FL+ T S L++N YP+F Y  ++  I LDYALF   + N   VD+   L Y+++F+A
Sbjct: 184 LLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDA 243

Query: 243 QIDAVYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTP 302
            +DA Y AMS L + ++ +VVTE+GWPSKG   E  A+ ENA +YN NL++ V+  +GTP
Sbjct: 244 IVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTP 303

Query: 303 LRPQDPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVNES 362
             P   +  +++ L+NE+ + GP SE+N+GLFY +   VY + L  A             
Sbjct: 304 KHPGTAVTTYIYELYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGA------------- 363

Query: 363 KAQVPTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSL 422
              +   D    +  QT+C+A      + LQAALD+ACG G  DC  +  G +CY P+ +
Sbjct: 364 -GAILAND----TTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDV 423

Query: 423 EAHASYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFP 463
            AH++YAFN+YYQKM +ASG+C F G A V T  P  G C FP
Sbjct: 424 VAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTCVFP 445

BLAST of Tan0013286 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.3e-91
Identity = 177/465 (38.06%), Postives = 276/465 (59.35%), Query Frame = 0

Query: 4   LTFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVL 63
           L FL+FLL     +     ++GVNYG+++DNLP  +  V LLK   I +++LF ++  V+
Sbjct: 10  LLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVI 69

Query: 64  TALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVD-P 123
            A +N+ + +V+   N  + + A++ +    +V+S +  FYPA+ I AI VGNEV     
Sbjct: 70  KAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGD 129

Query: 124 NNTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKP 183
                 L+PAM+NV ++L   +L   +K+S+  A++ L  SYP SSG F P L +  +K 
Sbjct: 130 QKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGD-TMKA 189

Query: 184 MLDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQID 243
           +L F     +  M++ YP+FAY       +L + LF+ N G VDSGNG KY+++F+AQ+D
Sbjct: 190 LLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVD 249

Query: 244 AVYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRP 303
           AV++A++A+ + D+++VV ETGWP  GD  E+G S +NA +Y GNL+  + +  GTPL P
Sbjct: 250 AVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMP 309

Query: 304 QDPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPL---TRADLESGAPTPVNES 363
              ++ +LF+L++E++K+G +SE+ +GLF PD    Y++ L   T+       PTP    
Sbjct: 310 GKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPTP---- 369

Query: 364 KAQVPTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSL 423
                    +P + G +WCV     + ++L   ++YACG+ G DC PIQPG  C+ PN++
Sbjct: 370 ---------TPKAAG-SWCVPKPGVSDDQLTGNINYACGQ-GIDCGPIQPGGACFEPNTV 429

Query: 424 EAHASYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTG 465
           +AHA+Y  N YYQ   R S  C F   A +    P YG C FP+G
Sbjct: 430 KAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFPSG 458

BLAST of Tan0013286 vs. NCBI nr
Match: KAG6577464.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 804.3 bits (2076), Expect = 5.6e-229
Identity = 403/461 (87.42%), Postives = 437/461 (94.79%), Query Frame = 0

Query: 5   TFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLT 64
           +F L LLS+ AL+EA +GWVGVNYGRIAD+LPPPEKVV+LLK QGIDRIKL+D+D+TVLT
Sbjct: 7   SFYLLLLSSIALSEA-AGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLT 66

Query: 65  ALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNN 124
           AL+NSNISVVVALPNELLSSAAADSSFTDNWVQS ISHFYP+TKIDAIAVGNEVFVDPNN
Sbjct: 67  ALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNN 126

Query: 125 TTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPML 184
           TTKFLVPAMKNVY+SL+KYNLHNSIKISSPIAL+AL SS+PTSSGSFK DL+EPVIKPML
Sbjct: 127 TTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPML 186

Query: 185 DFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAV 244
            FLRQTQSYLMVNAYPFFAYA+NSD+ISLDYALFR+NPGV+DSGNGLKYSSLFEAQIDAV
Sbjct: 187 AFLRQTQSYLMVNAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQIDAV 246

Query: 245 YAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQD 304
           +AAMSALKYDDVKVVVTETGWPSKGDEK++GASGENAASYNGNLVKRVLTGSGTPLRP D
Sbjct: 247 FAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPND 306

Query: 305 PLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN-ESKAQV 364
           PLNVFLFALFNENQK GPTSERNYGLFYP++++VY+IPLTRAD+ESG+PT VN  SKAQV
Sbjct: 307 PLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKAQV 366

Query: 365 PTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHA 424
           P+      SKGQTWCVANG  AAEKLQA LDYACGEGGADCRPIQPGSTCYNPNS+EAHA
Sbjct: 367 PS------SKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA 426

Query: 425 SYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTG 465
           SYAFNSYYQKMARASGTC FGGAAYVV+QPP+YGKCEFPTG
Sbjct: 427 SYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 460

BLAST of Tan0013286 vs. NCBI nr
Match: KAG7015534.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 802.4 bits (2071), Expect = 2.1e-228
Identity = 402/461 (87.20%), Postives = 437/461 (94.79%), Query Frame = 0

Query: 5   TFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLT 64
           +F L LLS+ AL+EA +GWVGVNYGRIAD+LPPPEKVV+LLK QGIDRIKL+D+D+TVLT
Sbjct: 7   SFYLLLLSSIALSEA-AGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLT 66

Query: 65  ALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNN 124
           AL+NSNISVVVALPNELLSSAAADSSFTDNWVQS ISHFYP+TKIDAIAVGNEVFVDPNN
Sbjct: 67  ALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNN 126

Query: 125 TTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPML 184
           TTKFLVPAMKNVY+SL+KYNLHNSIKISSPIAL+AL SS+PTSSGSFK DL+EPVIKPML
Sbjct: 127 TTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPML 186

Query: 185 DFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAV 244
            FLRQTQSYLMVNAYPFFAYA+NSD+ISLDYALFR+NPGV+DSGNGLKYSSLFEAQIDAV
Sbjct: 187 AFLRQTQSYLMVNAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQIDAV 246

Query: 245 YAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQD 304
           +AAMSALKYDDVKVVVTETGWPSKGDEK++GASGENAASYNGNLVKRVLTGSGTPLRP D
Sbjct: 247 FAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPND 306

Query: 305 PLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN-ESKAQV 364
           PL+VFLFALFNENQK GPTSERNYGLFYP++++VY+IPLTRAD+ESG+PT VN  SKAQV
Sbjct: 307 PLDVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKAQV 366

Query: 365 PTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHA 424
           P+      SKGQTWCVANG  AAEKLQA LDYACGEGGADCRPIQPGSTCYNPNS+EAHA
Sbjct: 367 PS------SKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA 426

Query: 425 SYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTG 465
           SYAFNSYYQKMARASGTC FGGAAYVV+QPP+YGKCEFPTG
Sbjct: 427 SYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 460

BLAST of Tan0013286 vs. NCBI nr
Match: XP_022932287.1 (glucan endo-1,3-beta-glucosidase 13-like [Cucurbita moschata])

HSP 1 Score: 802.4 bits (2071), Expect = 2.1e-228
Identity = 401/461 (86.98%), Postives = 437/461 (94.79%), Query Frame = 0

Query: 5   TFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLT 64
           +F L LLS+ AL+EA +GWVGVNYGRIAD+LPPPEKVV+LLK QGIDRIKL+D+D+TVLT
Sbjct: 7   SFYLLLLSSIALSEA-AGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLT 66

Query: 65  ALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNN 124
           AL+NSNISVVVALPNELLSSAAADSSFTDNWVQS ISHFYP+TKIDAIAVGNEVFVDPNN
Sbjct: 67  ALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNN 126

Query: 125 TTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPML 184
           TTKFLVPAMKNVY+SL+KYNLHNSIKISSPIAL+AL SS+PTSSGSFK DL+EPVIKPML
Sbjct: 127 TTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPML 186

Query: 185 DFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAV 244
            FLR+TQSYLM+NAYPFFAYA+NSD+ISLDYALFR+NPGV+DSGNGLKYSSLFEAQIDAV
Sbjct: 187 AFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQIDAV 246

Query: 245 YAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQD 304
           +AAMSALKYDDVKVVVTETGWPSKGDEK++GASGENAASYNGNLVKRVLTGSGTPLRP D
Sbjct: 247 FAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPND 306

Query: 305 PLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN-ESKAQV 364
           PLNVFLFALFNENQK GPTSERNYGLFYP++++VY+IPLTRAD+ESG+PT VN  SKAQV
Sbjct: 307 PLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKAQV 366

Query: 365 PTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHA 424
           P+      SKGQTWCVANG  AAEKLQA LDYACGEGGADCRPIQPGSTCYNPNS+EAHA
Sbjct: 367 PS------SKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA 426

Query: 425 SYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTG 465
           SYAFNSYYQKMARASGTC FGGAAYVV+QPP+YGKCEFPTG
Sbjct: 427 SYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 460

BLAST of Tan0013286 vs. NCBI nr
Match: XP_023551952.1 (glucan endo-1,3-beta-glucosidase 13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.1 bits (2034), Expect = 4.1e-224
Identity = 397/464 (85.56%), Postives = 433/464 (93.32%), Query Frame = 0

Query: 6   FLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLTA 65
           F L LLS+ AL++A +GWVGVNYGRIAD+LPPPEKVV+LLK QGIDRIKL+D+D+ VLTA
Sbjct: 4   FYLLLLSSIALSDA-AGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSAVLTA 63

Query: 66  LSNSNISVVVALPNELLSS----AAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVD 125
           L+NSNISVVVALPNELLSS    AAADSSFTDNWVQS ISHFYP+TKIDAIAVGNEVFVD
Sbjct: 64  LANSNISVVVALPNELLSSAAAAAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVD 123

Query: 126 PNNTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIK 185
           PNNTTKFL+PAMKNVY+SL+KYNLHNSIKISSPIAL+AL SS+PTSSGSFK DL+EPVIK
Sbjct: 124 PNNTTKFLIPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIK 183

Query: 186 PMLDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQI 245
           PML FLRQTQSYLMVNAYPFFAYA+NSD+ISLDYALFR+NPGV+DSGNGLKYSSLFEAQI
Sbjct: 184 PMLAFLRQTQSYLMVNAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQI 243

Query: 246 DAVYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLR 305
           DAV+AAMSALKYDDVKVVVTETGWPSKGDEK++GASGENAASYNGNLVKRVLTG GTPLR
Sbjct: 244 DAVFAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGRGTPLR 303

Query: 306 PQDPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN-ESK 365
           P D LNVFLFALFNEN+K GPTSERNYGLFYP++++VY+IPLTRAD+ESG+PT VN  SK
Sbjct: 304 PNDSLNVFLFALFNENRKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSK 363

Query: 366 AQVPTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLE 425
           AQVP+      SKGQTWCVANG  AAEKLQA LDYACGEGGADCRPIQPGSTCYNPNS+E
Sbjct: 364 AQVPS------SKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVE 423

Query: 426 AHASYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTG 465
           AHASYAFNSYYQKMARASGTC FGGAAYVV+QPP+YGKCEFPTG
Sbjct: 424 AHASYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 460

BLAST of Tan0013286 vs. NCBI nr
Match: XP_022965047.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima])

HSP 1 Score: 786.9 bits (2031), Expect = 9.2e-224
Identity = 395/460 (85.87%), Postives = 432/460 (93.91%), Query Frame = 0

Query: 6   FLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLTA 65
           +LL LLS+ AL+EA +GWVGVNYGRIAD+LP P+KVV+LLK QGIDRIKL+D+D+ VLTA
Sbjct: 9   YLLLLLSSIALSEA-AGWVGVNYGRIADDLPSPDKVVKLLKDQGIDRIKLYDTDSAVLTA 68

Query: 66  LSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNNT 125
           L+NSNISVVVALPNELLSSAAADSSFTD+WVQS ISHFYP+TKIDAIAVGNEVFVDPNNT
Sbjct: 69  LANSNISVVVALPNELLSSAAADSSFTDSWVQSSISHFYPSTKIDAIAVGNEVFVDPNNT 128

Query: 126 TKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPMLD 185
           TKFLVPAMKNVY+SL+KYNLH+SIKISSPIAL+AL SS+PTSSGSFK DL+EPVIKPML 
Sbjct: 129 TKFLVPAMKNVYTSLQKYNLHSSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPMLA 188

Query: 186 FLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAVY 245
           FLRQTQSYLMVNAYPFFAYA+NSD+ISLDYALF++NPGVVDSGNGLKYSSLFEAQIDAV+
Sbjct: 189 FLRQTQSYLMVNAYPFFAYASNSDKISLDYALFQDNPGVVDSGNGLKYSSLFEAQIDAVF 248

Query: 246 AAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQDP 305
           AAMSALKYDDVKVVVTETGWPSKGDEK++GAS ENAASYNGNLVKRVLTGSGTPLRP DP
Sbjct: 249 AAMSALKYDDVKVVVTETGWPSKGDEKDVGASEENAASYNGNLVKRVLTGSGTPLRPNDP 308

Query: 306 LNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN-ESKAQVP 365
           LNVFLFALFNENQK GPTSERNYGLFYP++++VY+IPLTRAD+ES APT VN  SKAQVP
Sbjct: 309 LNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESEAPTMVNGGSKAQVP 368

Query: 366 TGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHAS 425
           +      SKGQTWCVANG  AAE+LQA LDYACGEGGADCRPIQPGSTCYNPNS+EAHAS
Sbjct: 369 S------SKGQTWCVANGKTAAERLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 428

Query: 426 YAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTG 465
           YAFNSYYQKM RASGTC FGGAAYVV+QPP+YGKCEFPTG
Sbjct: 429 YAFNSYYQKMGRASGTCEFGGAAYVVSQPPQYGKCEFPTG 461

BLAST of Tan0013286 vs. ExPASy TrEMBL
Match: A0A6J1EW84 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111438639 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.0e-228
Identity = 401/461 (86.98%), Postives = 437/461 (94.79%), Query Frame = 0

Query: 5   TFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLT 64
           +F L LLS+ AL+EA +GWVGVNYGRIAD+LPPPEKVV+LLK QGIDRIKL+D+D+TVLT
Sbjct: 7   SFYLLLLSSIALSEA-AGWVGVNYGRIADDLPPPEKVVKLLKDQGIDRIKLYDTDSTVLT 66

Query: 65  ALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNN 124
           AL+NSNISVVVALPNELLSSAAADSSFTDNWVQS ISHFYP+TKIDAIAVGNEVFVDPNN
Sbjct: 67  ALANSNISVVVALPNELLSSAAADSSFTDNWVQSSISHFYPSTKIDAIAVGNEVFVDPNN 126

Query: 125 TTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPML 184
           TTKFLVPAMKNVY+SL+KYNLHNSIKISSPIAL+AL SS+PTSSGSFK DL+EPVIKPML
Sbjct: 127 TTKFLVPAMKNVYTSLQKYNLHNSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPML 186

Query: 185 DFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAV 244
            FLR+TQSYLM+NAYPFFAYA+NSD+ISLDYALFR+NPGV+DSGNGLKYSSLFEAQIDAV
Sbjct: 187 AFLRKTQSYLMINAYPFFAYASNSDKISLDYALFRDNPGVLDSGNGLKYSSLFEAQIDAV 246

Query: 245 YAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQD 304
           +AAMSALKYDDVKVVVTETGWPSKGDEK++GASGENAASYNGNLVKRVLTGSGTPLRP D
Sbjct: 247 FAAMSALKYDDVKVVVTETGWPSKGDEKDVGASGENAASYNGNLVKRVLTGSGTPLRPND 306

Query: 305 PLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN-ESKAQV 364
           PLNVFLFALFNENQK GPTSERNYGLFYP++++VY+IPLTRAD+ESG+PT VN  SKAQV
Sbjct: 307 PLNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESGSPTMVNGGSKAQV 366

Query: 365 PTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHA 424
           P+      SKGQTWCVANG  AAEKLQA LDYACGEGGADCRPIQPGSTCYNPNS+EAHA
Sbjct: 367 PS------SKGQTWCVANGKTAAEKLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHA 426

Query: 425 SYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTG 465
           SYAFNSYYQKMARASGTC FGGAAYVV+QPP+YGKCEFPTG
Sbjct: 427 SYAFNSYYQKMARASGTCDFGGAAYVVSQPPQYGKCEFPTG 460

BLAST of Tan0013286 vs. ExPASy TrEMBL
Match: A0A6J1HJA3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465026 PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 4.5e-224
Identity = 395/460 (85.87%), Postives = 432/460 (93.91%), Query Frame = 0

Query: 6   FLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLTA 65
           +LL LLS+ AL+EA +GWVGVNYGRIAD+LP P+KVV+LLK QGIDRIKL+D+D+ VLTA
Sbjct: 9   YLLLLLSSIALSEA-AGWVGVNYGRIADDLPSPDKVVKLLKDQGIDRIKLYDTDSAVLTA 68

Query: 66  LSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNNT 125
           L+NSNISVVVALPNELLSSAAADSSFTD+WVQS ISHFYP+TKIDAIAVGNEVFVDPNNT
Sbjct: 69  LANSNISVVVALPNELLSSAAADSSFTDSWVQSSISHFYPSTKIDAIAVGNEVFVDPNNT 128

Query: 126 TKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPMLD 185
           TKFLVPAMKNVY+SL+KYNLH+SIKISSPIAL+AL SS+PTSSGSFK DL+EPVIKPML 
Sbjct: 129 TKFLVPAMKNVYTSLQKYNLHSSIKISSPIALTALASSFPTSSGSFKQDLIEPVIKPMLA 188

Query: 186 FLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAVY 245
           FLRQTQSYLMVNAYPFFAYA+NSD+ISLDYALF++NPGVVDSGNGLKYSSLFEAQIDAV+
Sbjct: 189 FLRQTQSYLMVNAYPFFAYASNSDKISLDYALFQDNPGVVDSGNGLKYSSLFEAQIDAVF 248

Query: 246 AAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQDP 305
           AAMSALKYDDVKVVVTETGWPSKGDEK++GAS ENAASYNGNLVKRVLTGSGTPLRP DP
Sbjct: 249 AAMSALKYDDVKVVVTETGWPSKGDEKDVGASEENAASYNGNLVKRVLTGSGTPLRPNDP 308

Query: 306 LNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN-ESKAQVP 365
           LNVFLFALFNENQK GPTSERNYGLFYP++++VY+IPLTRAD+ES APT VN  SKAQVP
Sbjct: 309 LNVFLFALFNENQKPGPTSERNYGLFYPNQQRVYDIPLTRADVESEAPTMVNGGSKAQVP 368

Query: 366 TGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHAS 425
           +      SKGQTWCVANG  AAE+LQA LDYACGEGGADCRPIQPGSTCYNPNS+EAHAS
Sbjct: 369 S------SKGQTWCVANGKTAAERLQAGLDYACGEGGADCRPIQPGSTCYNPNSVEAHAS 428

Query: 426 YAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTG 465
           YAFNSYYQKM RASGTC FGGAAYVV+QPP+YGKCEFPTG
Sbjct: 429 YAFNSYYQKMGRASGTCEFGGAAYVVSQPPQYGKCEFPTG 461

BLAST of Tan0013286 vs. ExPASy TrEMBL
Match: A0A6J1KYS6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498785 PE=3 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 6.0e-221
Identity = 388/465 (83.44%), Postives = 418/465 (89.89%), Query Frame = 0

Query: 1   MGVLTFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDA 60
           M    FL  L SATA  E   G +GVNYGRIADNLPPP+KVV LLKQQGIDRIK++D+DA
Sbjct: 3   MPFFPFLYLLFSATAHGE--PGLIGVNYGRIADNLPPPDKVVHLLKQQGIDRIKVYDTDA 62

Query: 61  TVLTALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFV 120
            VLTALSNSNIS+VVALPN LLSSAAADSSFTDNWVQSKIS++YPATKI+A+AVGNEVFV
Sbjct: 63  AVLTALSNSNISIVVALPNHLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFV 122

Query: 121 DPNNTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVI 180
           DPNNTT FL+PAMKNVYSSL+KYNLH+SIK+SSPIAL+AL SSYPTSSGSFKPDL+EPVI
Sbjct: 123 DPNNTTNFLIPAMKNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVI 182

Query: 181 KPMLDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQ 240
           KPMLDFL QT+SYLMVNAYPFFAYAANSDQISLDYALFREN GVVD GNGLKY SLFEAQ
Sbjct: 183 KPMLDFLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQ 242

Query: 241 IDAVYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPL 300
           +DAVYAAM ALKYDD+KVVVTETGWPS G+EKE+GAS ENAASYNGNLV+RVLTG GTPL
Sbjct: 243 VDAVYAAMMALKYDDIKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGRGTPL 302

Query: 301 RPQDPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVNESK 360
           RP+DPL+VFLFALFNENQK GPTSERNYGLFYP+E+KVYEIP T AD+ S  PTPVN SK
Sbjct: 303 RPEDPLDVFLFALFNENQKPGPTSERNYGLFYPNEEKVYEIPFTLADMASETPTPVNGSK 362

Query: 361 AQVPTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLE 420
           AQVP       SKG TWCVANG+AAAEKL+  LDYACGEGGADCR IQPGSTCYNPNSLE
Sbjct: 363 AQVPP------SKGDTWCVANGNAAAEKLREGLDYACGEGGADCRAIQPGSTCYNPNSLE 422

Query: 421 AHASYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTGY 466
           AHASYAFNSYYQKM RASGTCYFGG AYVVTQPP+YG CEFPTGY
Sbjct: 423 AHASYAFNSYYQKMGRASGTCYFGGVAYVVTQPPQYGNCEFPTGY 459

BLAST of Tan0013286 vs. ExPASy TrEMBL
Match: A0A6J1GIP4 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454517 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 9.6e-219
Identity = 383/460 (83.26%), Postives = 416/460 (90.43%), Query Frame = 0

Query: 6   FLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLTA 65
           FL  L SATAL E   G +GVNYGRIADNLPPP+KVV LLKQQGIDRIK++D+DA VLTA
Sbjct: 8   FLFLLFSATALGE--PGLIGVNYGRIADNLPPPDKVVGLLKQQGIDRIKVYDTDAAVLTA 67

Query: 66  LSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNNT 125
           LSNSNIS+VVAL N+LLSSAAADSSFTDNWVQSKIS++YPATKI+A+AVGNEVFVDPNNT
Sbjct: 68  LSNSNISIVVALSNDLLSSAAADSSFTDNWVQSKISNYYPATKINAVAVGNEVFVDPNNT 127

Query: 126 TKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPMLD 185
           T FL+PAM+NVYSSL+KYNLH+SIK+SSPIAL+AL SSYPTSSGSFKPDL+EPVIKPMLD
Sbjct: 128 TNFLIPAMRNVYSSLQKYNLHDSIKVSSPIALTALASSYPTSSGSFKPDLIEPVIKPMLD 187

Query: 186 FLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAVY 245
           FL QT+SYLMVNAYPFFAYAANSDQISLDYALFREN GVVD GNGLKY SLFEAQ+DAVY
Sbjct: 188 FLHQTESYLMVNAYPFFAYAANSDQISLDYALFRENSGVVDPGNGLKYFSLFEAQVDAVY 247

Query: 246 AAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQDP 305
           AAM ALKYDDVKVVVTETGWPS G+EKE+GAS ENAASYNGNLV+RVLTG GTPLRP+DP
Sbjct: 248 AAMMALKYDDVKVVVTETGWPSIGEEKEVGASKENAASYNGNLVRRVLTGKGTPLRPEDP 307

Query: 306 LNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVNESKAQVPT 365
           L+VFLFALFNENQK GPTSERNYGLFYP+E++VY+IP T AD+ S  PTPVN SKAQVP 
Sbjct: 308 LDVFLFALFNENQKPGPTSERNYGLFYPNEEEVYQIPFTLADMASETPTPVNGSKAQVPP 367

Query: 366 GDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHASY 425
                 SKG TWCVANG+AAAEKL+  LDYACGEGGADC  IQPGSTCYNPNSLEAHASY
Sbjct: 368 ------SKGDTWCVANGNAAAEKLREGLDYACGEGGADCGAIQPGSTCYNPNSLEAHASY 427

Query: 426 AFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTGY 466
           AFNSYYQKM RASGTCYFGG AYVV QPP+YG CEFPTGY
Sbjct: 428 AFNSYYQKMGRASGTCYFGGVAYVVAQPPQYGNCEFPTGY 459

BLAST of Tan0013286 vs. ExPASy TrEMBL
Match: A0A6J1E1N7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111025610 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 6.7e-212
Identity = 371/461 (80.48%), Postives = 409/461 (88.72%), Query Frame = 0

Query: 5   TFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLT 64
           +FL   LSA ALA+   GWVGVNYGRIADNLPPP KVVELLK QGIDRIKLFD+D+TVL 
Sbjct: 6   SFLYLFLSAVALAD--EGWVGVNYGRIADNLPPPPKVVELLKAQGIDRIKLFDADSTVLA 65

Query: 65  ALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNN 124
           AL+NS IS VVALPNELLSSAAA  SFTD WVQ+ I+ FYP TKI+AIAVGNEVFVDPN+
Sbjct: 66  ALANSGISAVVALPNELLSSAAAAQSFTDQWVQTNIARFYPTTKIEAIAVGNEVFVDPNH 125

Query: 125 TTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPML 184
           +T+FLVPAM N+++SLEKYNL  SIKISSPIALSALGSSYPTSSGSF+ DL+EPVIKPML
Sbjct: 126 STQFLVPAMNNLHASLEKYNLSGSIKISSPIALSALGSSYPTSSGSFQEDLIEPVIKPML 185

Query: 185 DFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAV 244
           DFLR+TQSYLMVN YPFFAY  NSD+ISLDYAL R+ PGVVDSGNGL+YSSLFEAQ+DAV
Sbjct: 186 DFLRRTQSYLMVNVYPFFAYMDNSDKISLDYALLRDGPGVVDSGNGLRYSSLFEAQVDAV 245

Query: 245 YAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQD 304
           YAAMSALKYDDVK+VVTETGWPSKGD KE+GA+ ENAASYNGNLV+RVLTG GTPLRPQD
Sbjct: 246 YAAMSALKYDDVKLVVTETGWPSKGDTKEVGATEENAASYNGNLVQRVLTGGGTPLRPQD 305

Query: 305 PLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVNESKAQVP 364
            L VFLFALFNEN+KSGPTSERNYGLFYP+E++VY+IPLTRA ++ G  T VN SKAQVP
Sbjct: 306 SLKVFLFALFNENKKSGPTSERNYGLFYPNEQRVYDIPLTRAHVQGGPQTSVNGSKAQVP 365

Query: 365 TGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHAS 424
           +GD +P S+GQTWCVAN +A  EKLQA LDYACGEGGADCR IQPGS CYN NSLE HAS
Sbjct: 366 SGDLTPSSRGQTWCVANINAGVEKLQAGLDYACGEGGADCRSIQPGSMCYNSNSLEVHAS 425

Query: 425 YAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTGY 466
           YAFNSYYQK ARA GTCYFGGAAYVV+QPP YG+C FPTGY
Sbjct: 426 YAFNSYYQKNARARGTCYFGGAAYVVSQPPSYGECVFPTGY 464

BLAST of Tan0013286 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 671.4 bits (1731), Expect = 5.3e-193
Identity = 340/468 (72.65%), Postives = 387/468 (82.69%), Query Frame = 0

Query: 1   MGVLTFLLFLL---SATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFD 60
           M V    L +L   SA     A SG +GVNYGRIADNLP PEKVVELLK QGI+RIKL+D
Sbjct: 1   MAVFVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYD 60

Query: 61  SDATVLTALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNE 120
           ++ TVLTAL+NS I VVV+LPNE L+SAAAD S+TD WVQ  I  + PAT I+AIAVGNE
Sbjct: 61  TETTVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNE 120

Query: 121 VFVDPNNTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVE 180
           VFVDP NTT +LVPAMKNV SSL K+NL  SIKISSPIALSAL SSYP S+GSFKP+L+E
Sbjct: 121 VFVDPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIE 180

Query: 181 PVIKPMLDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLF 240
           PVIKPMLD LR+T S+LMVNAYPFFAYAAN+D+ISLDYALF+EN G VDSGNGLKY+SL 
Sbjct: 181 PVIKPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLL 240

Query: 241 EAQIDAVYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSG 300
           +AQIDAV+AAMSA+ ++DVK+VVTETGWPS GDE E+GA   NAA+YNG LVKRVLTG+G
Sbjct: 241 DAQIDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNG 300

Query: 301 TPLRPQDPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN 360
           TPL+P++PLNV+LFALFNENQK+GPTSERNYGLFYP+E KVY++ L      +G  TPVN
Sbjct: 301 TPLKPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSL------NGKSTPVN 360

Query: 361 ESKAQVPTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPN 420
           ++K +V      P   GQTWCVANG    EKLQ  LDYACGEGGADCRPIQPG+TCYNP 
Sbjct: 361 DNKEKVV--PVKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPE 420

Query: 421 SLEAHASYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTGY 466
           SLEAHASYAFNSYYQK AR  GTC FGGAAYVV+QPPKYGKCEFPTG+
Sbjct: 421 SLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPKYGKCEFPTGH 460

BLAST of Tan0013286 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 647.1 bits (1668), Expect = 1.1e-185
Identity = 322/456 (70.61%), Postives = 379/456 (83.11%), Query Frame = 0

Query: 10  LLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVLTALSNS 69
           +LS  +  +A SG VGVNYGRIA+NLP PEKVV LLK QGI+RIK+FD+D  VLTAL+NS
Sbjct: 11  ILSFLSAIDAHSGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTDKNVLTALANS 70

Query: 70  NISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPNNTTKFL 129
            I V+VALPNELLSSAA+  SF DNW+++ I  ++PAT+I+AIAVGNEVFVDP   T +L
Sbjct: 71  KIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVFVDP-TITPYL 130

Query: 130 VPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPMLDFLRQ 189
           V AMKN+++SL KY L  +IKISSPIALSAL +SYP SSGSFKP+L+EPV+KPML  L+Q
Sbjct: 131 VNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVVKPMLALLQQ 190

Query: 190 TQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDAVYAAMS 249
           T SYLMVNAYPFFAYAAN+D+ISLDYALF+EN G +DSG GLKY+SLF+AQIDAVYAA+S
Sbjct: 191 TSSYLMVNAYPFFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLFDAQIDAVYAALS 250

Query: 250 ALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQDPLNVF 309
           A+ +  VKV+VTETGWPS GDE E+GAS  NAA+YN  LVKRVLTG GTPLRP +PLNV+
Sbjct: 251 AVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTPLRPTEPLNVY 310

Query: 310 LFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVNESKAQVPTGDFS 369
           LFALFNENQK GPTSERNYGLFYP+E KVY +P T+      + TPVN ++ +VP     
Sbjct: 311 LFALFNENQKPGPTSERNYGLFYPNEGKVYNVPFTKK-----STTPVNGNRGKVPV---- 370

Query: 370 PLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPNSLEAHASYAFNS 429
              +G TWCV+NG  A EKLQ ALDYACGEGGADCRPIQPG+TCY+P SLEAHASYAFNS
Sbjct: 371 -THEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESLEAHASYAFNS 430

Query: 430 YYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTGY 466
           YYQK +R  GTC+FGGAA+VVTQPP+YGKCEFPTG+
Sbjct: 431 YYQKNSRRVGTCFFGGAAHVVTQPPRYGKCEFPTGH 455

BLAST of Tan0013286 vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 646.7 bits (1667), Expect = 1.4e-185
Identity = 330/457 (72.21%), Postives = 376/457 (82.28%), Query Frame = 0

Query: 1   MGVLTFLLFLL---SATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFD 60
           M V    L +L   SA     A SG +GVNYGRIADNLP PEKVVELLK QGI+RIKL+D
Sbjct: 1   MAVFVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYD 60

Query: 61  SDATVLTALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNE 120
           ++ TVLTAL+NS I VVV+LPNE L+SAAAD S+TD WVQ  I  + PAT I+AIAVGNE
Sbjct: 61  TETTVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNE 120

Query: 121 VFVDPNNTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVE 180
           VFVDP NTT +LVPAMKNV SSL K+NL  SIKISSPIALSAL SSYP S+GSFKP+L+E
Sbjct: 121 VFVDPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIE 180

Query: 181 PVIKPMLDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLF 240
           PVIKPMLD LR+T S+LMVNAYPFFAYAAN+D+ISLDYALF+EN G VDSGNGLKY+SL 
Sbjct: 181 PVIKPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLL 240

Query: 241 EAQIDAVYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSG 300
           +AQIDAV+AAMSA+ ++DVK+VVTETGWPS GDE E+GA   NAA+YNG LVKRVLTG+G
Sbjct: 241 DAQIDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNG 300

Query: 301 TPLRPQDPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN 360
           TPL+P++PLNV+LFALFNENQK+GPTSERNYGLFYP+E KVY++ L      +G  TPVN
Sbjct: 301 TPLKPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSL------NGKSTPVN 360

Query: 361 ESKAQVPTGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPN 420
           ++K +V      P   GQTWCVANG    EKLQ  LDYACGEGGADCRPIQPG+TCYNP 
Sbjct: 361 DNKEKVV--PVKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPE 420

Query: 421 SLEAHASYAFNSYYQKMARASGTCYFGGAAYVVTQPP 455
           SLEAHASYAFNSYYQK AR  GTC FGGAAYVV+QPP
Sbjct: 421 SLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPP 449

BLAST of Tan0013286 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 628.6 bits (1620), Expect = 3.9e-180
Identity = 311/468 (66.45%), Postives = 381/468 (81.41%), Query Frame = 0

Query: 4   LTFLLFLLSATALAEAGSGWVGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFDSDATVL 63
           L FLL LLS  +L++AGS  +GVNYGRI+D LP   KVV+LLK QGI R+K+FD+D +VL
Sbjct: 7   LIFLLLLLSPFSLSDAGS--IGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSVL 66

Query: 64  TALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNEVFVDPN 123
            ALS S I V V LPNELL SAA  +SF  +WV+  ++ ++P+T+I++IAVGNEVFVD +
Sbjct: 67  KALSGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDTH 126

Query: 124 NTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVEPVIKPM 183
           NTT FL+PAM+N++ +L  +NLH+ IKISSP+ALSAL +SYP+SSGSF+P+L++ VIKPM
Sbjct: 127 NTTSFLIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKPM 186

Query: 184 LDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLFEAQIDA 243
           LDFLR+T S LM+N YPFFAY  NSD I LDYAL RENPG+VDSGNGL+Y +LF+AQIDA
Sbjct: 187 LDFLRETGSRLMINVYPFFAYEGNSDVIPLDYALLRENPGMVDSGNGLRYFNLFDAQIDA 246

Query: 244 VYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSGTPLRPQ 303
           V+AAMSALKYDD++++VTETGWPSKGDE E+GA+  NAASYNGNL++R+LT  GTPLRP+
Sbjct: 247 VFAAMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRGGTPLRPK 306

Query: 304 DPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLE---SGAPTPV--NE 363
             L V+LFALFNEN+K GPTSERNYGLF+PDEKKVY+IP T   L+    G  TPV   +
Sbjct: 307 ADLTVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRDGGHTPVTGGD 366

Query: 364 SKAQVP-TGDFSPLSKGQTWCVANGHAAAEKLQAALDYACGEGGADCRPIQPGSTCYNPN 423
              + P +G  S    G TWCVANG A  E+LQ  LDYACGEGGADCRPIQPG+ CY+P+
Sbjct: 367 QVTKPPMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYSPD 426

Query: 424 SLEAHASYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKCEFPTGY 466
           +LEAHAS+AFNSYYQK  RA G+CYFGGAAYVV+QPPKYG+CEFPTGY
Sbjct: 427 TLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPPKYGRCEFPTGY 472

BLAST of Tan0013286 vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 371.7 bits (953), Expect = 8.6e-103
Identity = 193/476 (40.55%), Postives = 296/476 (62.18%), Query Frame = 0

Query: 1   MGVLTFLLFLLSATALAEAGSGW---VGVNYGRIADNLPPPEKVVELLKQQGIDRIKLFD 60
           MG    L+F +  + LA    G    +G+ YGR ADNLP P +V EL++   I  ++++D
Sbjct: 1   MGQRLNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYD 60

Query: 61  SDATVLTALSNSNISVVVALPNELLSSAAADSSFTDNWVQSKISHFYPATKIDAIAVGNE 120
           ++  VL A +N+ I +++ +PN  L + A   S  D W+ + I  +YP+TKI +I+VG E
Sbjct: 61  ANIDVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLE 120

Query: 121 VFVDPNNTTKFLVPAMKNVYSSLEKYNLHNSIKISSPIALSALGSSYPTSSGSFKPDLVE 180
           V   P+N T  ++PAM+N++++L+K  L   IKISS  +L+ L  S+P SS SF      
Sbjct: 121 VTEAPDNATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HS 180

Query: 181 PVIKPMLDFLRQTQSYLMVNAYPFFAYAANSDQISLDYALFRENPGVVDSGNGLKYSSLF 240
             +KPML+FL + +S  M++ YP++AY  +++++ L+YALF  +  VVD   GL YS++F
Sbjct: 181 AFLKPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMF 240

Query: 241 EAQIDAVYAAMSALKYDDVKVVVTETGWPSKGDEKELGASGENAASYNGNLVKRVLTGSG 300
           +AQ+DA+Y A++A+ +  VKV+VTE+GWPSKG  KE  A+ ENA +YN NL++ V+   G
Sbjct: 241 DAQLDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPG 300

Query: 301 TPLRPQDPLNVFLFALFNENQKSGPTSERNYGLFYPDEKKVYEIPLTRADLESGAPTPVN 360
           TP +P + ++V+LF+LFNEN+K G  SERN+G+FY +   VY +  T  +    +PT  N
Sbjct: 301 TPAKPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPT--N 360

Query: 361 ESKAQVPTGDFSPLSKGQT--------------WCVANGHAAAEKLQAALDYACGEGGAD 420
            +    P+   SP+  G +              WC+A+  A+  +LQ ALD+ACG G  D
Sbjct: 361 STTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVD 420

Query: 421 CRPIQPGSTCYNPNSLEAHASYAFNSYYQKMARASGTCYFGGAAYVVTQPPKYGKC 460
           C  +QP   C+ P+++ +HASYAFN+YYQ+   +S  C F GA+  V + P YG C
Sbjct: 421 CSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNC 473

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYE51.2e-10140.55Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9FJU94.6e-10140.99Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Q9M0691.8e-9741.65Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q9ZU917.9e-9339.31Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Q94G863.3e-9138.06Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6577464.15.6e-22987.42Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. soro... [more]
KAG7015534.12.1e-22887.20Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_022932287.12.1e-22886.98glucan endo-1,3-beta-glucosidase 13-like [Cucurbita moschata][more]
XP_023551952.14.1e-22485.56glucan endo-1,3-beta-glucosidase 13-like [Cucurbita pepo subsp. pepo][more]
XP_022965047.19.2e-22485.87glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1EW841.0e-22886.98(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111438639 P... [more]
A0A6J1HJA34.5e-22485.87(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465026 PE=... [more]
A0A6J1KYS66.0e-22183.44(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111498785 PE=... [more]
A0A6J1GIP49.6e-21983.26(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454517 P... [more]
A0A6J1E1N76.7e-21280.48(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111025610 ... [more]
Match NameE-valueIdentityDescription
AT5G55180.15.3e-19372.65O-Glycosyl hydrolases family 17 protein [more]
AT4G26830.11.1e-18570.61O-Glycosyl hydrolases family 17 protein [more]
AT5G55180.21.4e-18572.21O-Glycosyl hydrolases family 17 protein [more]
AT2G05790.13.9e-18066.45O-Glycosyl hydrolases family 17 protein [more]
AT4G29360.18.6e-10340.55O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 376..461
e-value: 5.3E-49
score: 178.8
IPR012946X8 domainPFAMPF07983X8coord: 376..447
e-value: 1.3E-22
score: 80.3
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 23..349
e-value: 4.4E-128
score: 428.7
NoneNo IPR availableGENE3D1.20.58.1040coord: 371..461
e-value: 2.4E-11
score: 45.9
NoneNo IPR availablePANTHERPTHR32227:SF299O-GLYCOSYL HYDROLASES FAMILY 17 PROTEINcoord: 13..458
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 24..341
e-value: 1.9E-93
score: 313.5
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 256..269
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 13..458
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 24..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0013286.1Tan0013286.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds