Tan0012794 (gene) Snake gourd v1

BLAST of Tan0012794 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 2.2e-176
Identity = 312/509 (61.30%), Postives = 395/509 (77.60%), Query Frame = 0

Query: 11  LQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRR-SPPKDMSEFKKMVIQFAM 70
           L  F LS ++F  +    +  WC KT  P+ C+YF +     +  K  ++F K+ +Q A+
Sbjct: 11  LVLFSLSHTSF-GYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLAL 70

Query: 71  EKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCTEFDIQTWLS 130
           E+A++ +S    +GSKCRNE+E+AAW DC +LY+ T+ +LN T +SS  CT+ D QTWLS
Sbjct: 71  ERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLS 130

Query: 131 TALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKETYKKGFPSW 190
           TALTNLETCR    +L V +YVLPL+ SNNVTKLISN+L++N       + +YK GFP+W
Sbjct: 131 TALTNLETCRASLEDLGVPEYVLPLL-SNNVTKLISNTLSLNKVPY--NEPSYKDGFPTW 190

Query: 191 LSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRGVYEENL 250
           +  GDRKLLQ++  +A++VVAQDGSG+ KT+  A+  AA R    R+VI +K G Y EN+
Sbjct: 191 VKPGDRKLLQTTP-RANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYVIYIKAGTYNENI 250

Query: 251 DIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGITFRNTAGPE 310
           ++  K+KNIM VGDG+  T ITG++SVGGG+TTF SATVAV G+ F+AR IT RNTAGP 
Sbjct: 251 EV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPN 310

Query: 311 NHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNC 370
           NHQAVALRSG+DLSVFYRC+FEGYQDTLYVHSQRQFY+EC IYGTVDFIFGNAAVVLQNC
Sbjct: 311 NHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNC 370

Query: 371 MIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEY 430
            I+AR+P N + N +TAQGRTDPNQ+TGI IHN RV A  DLKPV+S+VKT+LGRPWK+Y
Sbjct: 371 NIFARKPPN-RTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQY 430

Query: 431 SRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKWKGYRVISNS 490
           SRTV++KT+LDSL++PAGW+EW+GDFALNTLYY EY N GPGSST+ RVKW+GY V+++ 
Sbjct: 431 SRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSP 490

Query: 491 TEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           ++ S+FTV NFIAG SWLP T VPF  GL
Sbjct: 491 SQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of Tan0012794 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 599.4 bits (1544), Expect = 4.0e-170
Identity = 300/508 (59.06%), Postives = 386/508 (75.98%), Query Frame = 0

Query: 15  LLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPP-KDMSEFKKMVIQFAMEKAS 74
           +L +S  S +    +  WC +T  P+ C+YF +H   + P K  SEF K+ ++  +++A 
Sbjct: 16  ILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAI 75

Query: 75  SVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCTEFDIQTWLSTALT 134
             ++HA  +G KCR+ +E+AAW DC+KLY  T+ ++N T+D + KC++ D QTWLSTALT
Sbjct: 76  LAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALT 135

Query: 135 NLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNAS---TWPGKETYKKGFPSWL 194
           NL+TCR GF EL V+D VLPL+ SNNV+ L+ N+LA+N      T P K+    GFPSW+
Sbjct: 136 NLDTCRAGFLELGVTDIVLPLM-SNNVSNLLCNTLAINKVPFNYTPPEKD----GFPSWV 195

Query: 195 SGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRGVYEENLD 254
             GDRKLLQSS+ K + VVA+DGSG+ KT+  A++ A+     GRFVI VK+GVY ENL+
Sbjct: 196 KPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENLE 255

Query: 255 IGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGITFRNTAGPEN 314
           I  + KN+ML GDG+  T ITG++SVGGG+TTFNSATVA  G+GF+ARGITFRNTAG  N
Sbjct: 256 I--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASN 315

Query: 315 HQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCM 374
            QAVALRSG+DLSVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA VLQNC 
Sbjct: 316 EQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCN 375

Query: 375 IYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYS 434
           I+ARRP   + N +TAQGR+DPNQNTGI IHNSRV A  DL+PV  + KTYLGRPW++YS
Sbjct: 376 IFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYS 435

Query: 435 RTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKWKGYRVISNST 494
           RTVFMKT LDSL+DP GWLEW+G+FAL TL+Y E++N GPG+STS RV W G+RV+ +++
Sbjct: 436 RTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSAS 495

Query: 495 EASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           EAS+FTV  F+AG SW+P + VPF  GL
Sbjct: 496 EASKFTVGTFLAGGSWIP-SSVPFTSGL 511

BLAST of Tan0012794 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 4.4e-161
Identity = 290/524 (55.34%), Postives = 370/524 (70.61%), Query Frame = 0

Query: 4   SIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCK-YFFSHGRRSPPKDMSEFKK 63
           S+ +L +L   L   +  ++  S  ID WC KT YP  CK YF  H     P  +SEF+ 
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRV 72

Query: 64  MVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLD-----SST 123
           ++++ AM++A S           C + K++A  ADC+ LY +T+ QLN TL      +  
Sbjct: 73  LLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGR 132

Query: 124 KCTEFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWP 183
           +CT+FD QTWLSTALTN ETCR G ++LNVSD+  P++++  ++ LISN LAVN A    
Sbjct: 133 RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTA 192

Query: 184 GKETY----KKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRK-T 243
           GK        KGFP+W+S  +R+LLQ  SV+A+LVVA+DGSG  KTV AA++ A +RK T
Sbjct: 193 GKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVT 252

Query: 244 DGRFVIQVKRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 303
            GRFVI VKRG+Y+ENL++     NIMLVGDGMR+T ITG RSV GG TT++SAT  + G
Sbjct: 253 SGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEG 312

Query: 304 EGFMARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 363
             F+A+GI F+NTAGP   QAVALRS +DLS+FYRC+ EGYQDTL VHSQRQFY+ECYIY
Sbjct: 313 LHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIY 372

Query: 364 GTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 423
           GTVDFIFGNAAVV QNC+I  R P+ GQ N +TAQGRTD  QNTGISIHNS ++   DLK
Sbjct: 373 GTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLK 432

Query: 424 PVESTVKTYLGRPWKEYSRTVFMKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGP 483
           PV  +VKTY+GRPW  YSRTV +KTY+DS+V P GW  W     + L+TL+Y EYKNIGP
Sbjct: 433 PVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGP 492

Query: 484 GSSTSQRVKWKGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPF 515
            SST  RV+WKG+ V+S +++AS F+V  FIAG +WLPG+ +PF
Sbjct: 493 ASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

BLAST of Tan0012794 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 9.8e-161
Identity = 290/530 (54.72%), Postives = 370/530 (69.81%), Query Frame = 0

Query: 4   SIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCK-YFFSHGRRSPPKDMSEFKK 63
           SI  L L+   LL     +         WC KT YP+ CK YF +H     P  +SEF+ 
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRV 68

Query: 64  MVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTL-------DS 123
           M+++ AM++A S  +     G  C + K++A  ADC+ LY +TI QLN TL        +
Sbjct: 69  MLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGA 128

Query: 124 STKCTEFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNAST 183
           +  CT+FD QTWLSTALTN ETCR G ++LNV+D++ P++++  ++ LISN LAVN A  
Sbjct: 129 AKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALL 188

Query: 184 WPGKE----TYKKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRK 243
             G +      +KGFP+WLS  D++LL+  +V+A+LVVA+DGSG   TV AA++ A +RK
Sbjct: 189 TAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAGRRK 248

Query: 244 -TDGRFVIQVKRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAV 303
            T GRFVI VKRG+Y+EN+++     +IMLVGDGMR T ITG RSV GG TT+NSAT  +
Sbjct: 249 VTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGI 308

Query: 304 TGEGFMARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECY 363
            G  F+A+GITFRNTAGP   QAVALRS +DLS+FY+C+ EGYQDTL VHSQRQFY+ECY
Sbjct: 309 EGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECY 368

Query: 364 IYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDD 423
           IYGTVDFIFGNAA V QNC+I  RRP+ GQ N +TAQGR DP QNTGISIHNSR++   D
Sbjct: 369 IYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPD 428

Query: 424 LKPVESTVKTYLGRPWKEYSRTVFMKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNI 483
           LKPV  TVKTY+GRPW ++SRTV ++TYLD++V P GW  W     F L+TL+Y EYKN 
Sbjct: 429 LKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNT 488

Query: 484 GPGSSTSQRVKWKGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           GP SST  RV WKG+ V+  +++AS FTV  FIAG +WLP T +PF  GL
Sbjct: 489 GPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Tan0012794 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.3e-156
Identity = 280/515 (54.37%), Postives = 358/515 (69.51%), Query Frame = 0

Query: 25  VSNGIDWWCRKTTYPETCKYFFSHGRRS-----PPKDMSEFKKMVIQFAMEKASSVESHA 84
           VS  + WWC KT + ETC Y+F    ++     PP+  SEF +M+++ A+++A    S  
Sbjct: 80  VSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQT 139

Query: 85  KGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTL-------DSSTKCTEFDIQTWLSTAL 144
              G  C N + +AAW+DC+ L+QNT+ QLN TL        S  KCT+FD QTWLSTA 
Sbjct: 140 VKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQ 199

Query: 145 TNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKET----YKKGFPS 204
           TN+ETCR+G  +LNVSD+V+P+I++ N++ LI N LAVN         T    +K+ FPS
Sbjct: 200 TNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPS 259

Query: 205 WLSGGDRKLLQSSSV---KADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRGVY 264
           W+S  +R+LL S+S+      LVVAQD SG  +++ AA+  AA+R+   RFVI VK+GVY
Sbjct: 260 WVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVY 319

Query: 265 EENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGITFRNT 324
            EN+D+GN   NIMLVGDG R T IT  RSV  G TT+NSAT    G+ F+A+ +TF NT
Sbjct: 320 RENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINT 379

Query: 325 AGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVV 384
           AGP   QAVA+RS +DLSVFYR    G+QDTLY+HSQRQF++ECYI GT+DFIFGNAAVV
Sbjct: 380 AGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVV 439

Query: 385 LQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRP 444
            QNCMI  RRP++GQ N +TAQGR DP QNTGI+IH+SR++A  DLKPV    KTYLGRP
Sbjct: 440 FQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRP 499

Query: 445 WKEYSRTVFMKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPGSSTSQRVKWKGY 504
           W+ YSR   MKTY+D+ + P GW  W    +FALNT++YGEYKN GPGSST  RV+WKG+
Sbjct: 500 WQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGF 559

Query: 505 RVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
             I+++  AS FTV + IAG SWLP T VPF  GL
Sbjct: 560 HAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Tan0012794 vs. NCBI nr
Match: XP_038882414.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 953.0 bits (2462), Expect = 1.1e-273
Identity = 465/518 (89.77%), Postives = 487/518 (94.02%), Query Frame = 0

Query: 1   MEKSIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSEF 60
           ME+S+GV F L FFLLSS+AFS H SNGIDWWCRKTTYPETCKYFF+ G ++ PKDM+EF
Sbjct: 1   MERSLGVFFFLHFFLLSSAAFSKHASNGIDWWCRKTTYPETCKYFFNQGSKAAPKDMTEF 60

Query: 61  KKMVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCT 120
           KKMVIQFAME+A   ESH KGVGSKCRN KE+AAWADCLKLYQNTIFQLN TLDS+TKCT
Sbjct: 61  KKMVIQFAMERALGAESHTKGVGSKCRNGKEKAAWADCLKLYQNTIFQLNQTLDSATKCT 120

Query: 121 EFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKE 180
           EFDIQTWLSTALTNLETCRTGFAELNVSDY+L LI SNNVTKLISNSLA+NNAS   GKE
Sbjct: 121 EFDIQTWLSTALTNLETCRTGFAELNVSDYILQLIMSNNVTKLISNSLAINNASAGLGKE 180

Query: 181 TYKKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQV 240
           TYKKGFPSWLSGGDR+LLQSSS K DLVVAQDGSG+H TV AALEEAAKRKT+GRFVIQV
Sbjct: 181 TYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNHTTVGAALEEAAKRKTNGRFVIQV 240

Query: 241 KRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGI 300
           KRGVY+ENL+IG+KMKNIML+GDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGF+ARGI
Sbjct: 241 KRGVYQENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGI 300

Query: 301 TFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFG 360
           TFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFG
Sbjct: 301 TFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFG 360

Query: 361 NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKT 420
           NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKT
Sbjct: 361 NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKT 420

Query: 421 YLGRPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKW 480
           YLGRPWKEYSRTVFMKTYLDSLVDP GWLEW+GDFAL+TLYYGEY NIGP S TSQRV W
Sbjct: 421 YLGRPWKEYSRTVFMKTYLDSLVDPTGWLEWDGDFALDTLYYGEYNNIGPRSPTSQRVNW 480

Query: 481 KGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           KGYRVI NSTEASEFTVQNFIAGQSWLP TEVPF PGL
Sbjct: 481 KGYRVIRNSTEASEFTVQNFIAGQSWLPDTEVPFTPGL 518

BLAST of Tan0012794 vs. NCBI nr
Match: XP_022141175.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 947.6 bits (2448), Expect = 4.5e-272
Identity = 460/518 (88.80%), Postives = 485/518 (93.63%), Query Frame = 0

Query: 1   MEKSIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSEF 60
           MEKS GVLF L FF LSS AFS H SNGIDWWC KTTYPETCKYFF+HG  + PKDMSEF
Sbjct: 40  MEKSFGVLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEF 99

Query: 61  KKMVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCT 120
           KKMV +FAME+A   ESHAKGVGSKCRNEKE+AAWADCL+LYQNTI QLNHTLDSS+KCT
Sbjct: 100 KKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCT 159

Query: 121 EFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKE 180
           EFDIQTWLSTALTNLETC TGFAE NVSD+VLPLI SNNVTKLISNSLA+NNAS WPGKE
Sbjct: 160 EFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKE 219

Query: 181 TYKKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQV 240
           TYK+GFPSWL GGDR+LLQSSSVK DLVVAQDGSGDH+TV AALE AAKRKT GRFVI V
Sbjct: 220 TYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHV 279

Query: 241 KRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGI 300
           KRGVYEENL+IGNKMKNIMLVGDGMRFT ITG+RSV GGSTTF+SATVAVTGEGF+ARGI
Sbjct: 280 KRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGI 339

Query: 301 TFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFG 360
           TFRNTAGPENHQAVALRSGAD SVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVDFIFG
Sbjct: 340 TFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFG 399

Query: 361 NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKT 420
           NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VESTVKT
Sbjct: 400 NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKT 459

Query: 421 YLGRPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKW 480
           YLGRPWKEYSRTVFM+TYLDSLVDPAGWLEW+G+FALNTLYYGEYKN+GPGSSTSQRVKW
Sbjct: 460 YLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKW 519

Query: 481 KGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           +GY +++NSTEAS+FTVQNFIAGQSWLPGTEVPF PGL
Sbjct: 520 RGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 557

BLAST of Tan0012794 vs. NCBI nr
Match: XP_023551648.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 943.3 bits (2437), Expect = 8.6e-271
Identity = 461/517 (89.17%), Postives = 484/517 (93.62%), Query Frame = 0

Query: 2   EKSIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSEFK 61
           + ++GVLF L FFLLSSSAFS HVSNGI WWC KTTYPETCKYF+ H    PPKDMSEFK
Sbjct: 3   KSTLGVLFFLHFFLLSSSAFSKHVSNGIHWWCTKTTYPETCKYFYGHANNPPPKDMSEFK 62

Query: 62  KMVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCTE 121
           KM I+FAM+ A SVE H K VGS+CRN KER AWADCLKLYQNTIFQL  TLDSS K TE
Sbjct: 63  KMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLERTLDSSAKSTE 122

Query: 122 FDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKET 181
           FDIQTWLSTALTN+ETCRTGFAELNV D VLPLITSNNVT+LISNSLAVNNASTW GKET
Sbjct: 123 FDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSNNVTQLISNSLAVNNASTWQGKET 182

Query: 182 YKKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVK 241
           YK+GFPSWLSGGDRKLLQSSS+  DLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVK
Sbjct: 183 YKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVK 242

Query: 242 RGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGIT 301
           RGVYEENL+IGNKMKNIMLVGDGMRFTFITG+RSVGGGSTTFNSATVAVTGEGF+A+GIT
Sbjct: 243 RGVYEENLEIGNKMKNIMLVGDGMRFTFITGSRSVGGGSTTFNSATVAVTGEGFIAQGIT 302

Query: 302 FRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGN 361
           FRNTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGN
Sbjct: 303 FRNTAGPENHQAVALRSGADRSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGN 362

Query: 362 AAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTY 421
           AAVVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VESTVKT+
Sbjct: 363 AAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTF 422

Query: 422 LGRPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKWK 481
           LGRPWKEYSRTVF+KTYLD+LVDPAGWLEWNGDFALNTLYYGEYKNIGP SST QRV+WK
Sbjct: 423 LGRPWKEYSRTVFIKTYLDTLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWK 482

Query: 482 GYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           GY VISNSTEASEF+VQNFI+GQSWLPGTEVPFIPG+
Sbjct: 483 GYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 519

BLAST of Tan0012794 vs. NCBI nr
Match: XP_022943089.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 941.8 bits (2433), Expect = 2.5e-270
Identity = 460/515 (89.32%), Postives = 484/515 (93.98%), Query Frame = 0

Query: 4   SIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSEFKKM 63
           ++GVLFL+ FFLLSSSAFS HVSNGI WWC KTTYPETCKYF+ H    PPKDMSEFKKM
Sbjct: 5   TLGVLFLIHFFLLSSSAFSKHVSNGIHWWCTKTTYPETCKYFYGHANNPPPKDMSEFKKM 64

Query: 64  VIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCTEFD 123
            I+FAM+ A SVE H K VGS+CRN KER AWADCLKLYQNTIFQL  TLDSS K TEFD
Sbjct: 65  AIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLERTLDSSAKSTEFD 124

Query: 124 IQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKETYK 183
           IQTWLSTALTN+ETCRTGFAELNV D VLPLITSNNVT+LISNSLAVNNASTW GKETYK
Sbjct: 125 IQTWLSTALTNVETCRTGFAELNVVDNVLPLITSNNVTQLISNSLAVNNASTWQGKETYK 184

Query: 184 KGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRG 243
           +GFPSWLSGGDRKLLQSSS+  DLVVAQDGSGDHKTVAAALEEAAKR+TDGRFVIQVKRG
Sbjct: 185 QGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRRTDGRFVIQVKRG 244

Query: 244 VYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGITFR 303
           VYEENL+IGNKMKNIMLVGDGMRFTFITG+RSVGGGSTTFNSATVAVTGEGF+A+GITFR
Sbjct: 245 VYEENLEIGNKMKNIMLVGDGMRFTFITGSRSVGGGSTTFNSATVAVTGEGFIAQGITFR 304

Query: 304 NTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 363
           NTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA
Sbjct: 305 NTAGPENHQAVALRSGADRSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 364

Query: 364 VVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLG 423
           VVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VESTVKT+LG
Sbjct: 365 VVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTFLG 424

Query: 424 RPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKWKGY 483
           RPWKEYSRTVF+KTYLD+LVDPAGWLEWNGDFALNTLYYGEYKNIGP SST QRV+WKGY
Sbjct: 425 RPWKEYSRTVFIKTYLDTLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGY 484

Query: 484 RVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
            VISNSTEASEF+VQNFI+GQSWLPGTEVPFIPG+
Sbjct: 485 HVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 519

BLAST of Tan0012794 vs. NCBI nr
Match: KAG6600170.1 (Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 939.9 bits (2428), Expect = 9.5e-270
Identity = 460/515 (89.32%), Postives = 482/515 (93.59%), Query Frame = 0

Query: 4   SIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSEFKKM 63
           ++GVLF L FFLLSSSAFS HVSNGI WWC KTTYPETCKYF+ H    PPKDMSEFKKM
Sbjct: 5   TLGVLFFLHFFLLSSSAFSKHVSNGIHWWCTKTTYPETCKYFYGHANNPPPKDMSEFKKM 64

Query: 64  VIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCTEFD 123
            I+FAM+ A SVE H K VGS+CRN KER AWADCLKLYQNTIFQL  TLDSS K TE D
Sbjct: 65  AIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLERTLDSSAKSTEVD 124

Query: 124 IQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKETYK 183
           IQTWLSTALTN+ETCRTGFAELNV D VLPLITSNNVTKLISNSLAVNNASTW GKETYK
Sbjct: 125 IQTWLSTALTNVETCRTGFAELNVVDNVLPLITSNNVTKLISNSLAVNNASTWQGKETYK 184

Query: 184 KGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRG 243
           +GFPSWLSGGDRKLLQSSS+  DLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRG
Sbjct: 185 QGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRG 244

Query: 244 VYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGITFR 303
           VYEENL+IGNKMKNIMLVGDGMRFTFITG+RSVGGGSTTFNSATVAVTGEGF+A+GITFR
Sbjct: 245 VYEENLEIGNKMKNIMLVGDGMRFTFITGSRSVGGGSTTFNSATVAVTGEGFIAQGITFR 304

Query: 304 NTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 363
           NTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA
Sbjct: 305 NTAGPENHQAVALRSGADRSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 364

Query: 364 VVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLG 423
           VVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VESTVKT+LG
Sbjct: 365 VVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTFLG 424

Query: 424 RPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKWKGY 483
           RPWKEYSRTVF+KTYLD+LVDPAGWLEWNGDFALNT+YYGEYKNIGP SST QRV+WKGY
Sbjct: 425 RPWKEYSRTVFIKTYLDTLVDPAGWLEWNGDFALNTVYYGEYKNIGPRSSTDQRVQWKGY 484

Query: 484 RVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
            VISNSTEASEF+VQNFI+GQSWLPGTEVPFIPG+
Sbjct: 485 HVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 519

BLAST of Tan0012794 vs. ExPASy TrEMBL
Match: A0A6J1CH86 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111011631 PE=3 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.2e-272
Identity = 460/518 (88.80%), Postives = 485/518 (93.63%), Query Frame = 0

Query: 1   MEKSIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSEF 60
           MEKS GVLF L FF LSS AFS H SNGIDWWC KTTYPETCKYFF+HG  + PKDMSEF
Sbjct: 40  MEKSFGVLFFLLFFSLSSPAFSKHASNGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEF 99

Query: 61  KKMVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCT 120
           KKMV +FAME+A   ESHAKGVGSKCRNEKE+AAWADCL+LYQNTI QLNHTLDSS+KCT
Sbjct: 100 KKMVTKFAMERALRAESHAKGVGSKCRNEKEKAAWADCLELYQNTILQLNHTLDSSSKCT 159

Query: 121 EFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKE 180
           EFDIQTWLSTALTNLETC TGFAE NVSD+VLPLI SNNVTKLISNSLA+NNAS WPGKE
Sbjct: 160 EFDIQTWLSTALTNLETCYTGFAEFNVSDHVLPLIMSNNVTKLISNSLAINNASAWPGKE 219

Query: 181 TYKKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQV 240
           TYK+GFPSWL GGDR+LLQSSSVK DLVVAQDGSGDH+TV AALE AAKRKT GRFVI V
Sbjct: 220 TYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHV 279

Query: 241 KRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGI 300
           KRGVYEENL+IGNKMKNIMLVGDGMRFT ITG+RSV GGSTTF+SATVAVTGEGF+ARGI
Sbjct: 280 KRGVYEENLEIGNKMKNIMLVGDGMRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGI 339

Query: 301 TFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFG 360
           TFRNTAGPENHQAVALRSGAD SVFYRC FEGYQDTLYVHSQRQFYKECYIYGTVDFIFG
Sbjct: 340 TFRNTAGPENHQAVALRSGADFSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFG 399

Query: 361 NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKT 420
           NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VESTVKT
Sbjct: 400 NAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKT 459

Query: 421 YLGRPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKW 480
           YLGRPWKEYSRTVFM+TYLDSLVDPAGWLEW+G+FALNTLYYGEYKN+GPGSSTSQRVKW
Sbjct: 460 YLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKW 519

Query: 481 KGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           +GY +++NSTEAS+FTVQNFIAGQSWLPGTEVPF PGL
Sbjct: 520 RGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVPFTPGL 557

BLAST of Tan0012794 vs. ExPASy TrEMBL
Match: A0A6J1FT96 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447927 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.2e-270
Identity = 460/515 (89.32%), Postives = 484/515 (93.98%), Query Frame = 0

Query: 4   SIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSEFKKM 63
           ++GVLFL+ FFLLSSSAFS HVSNGI WWC KTTYPETCKYF+ H    PPKDMSEFKKM
Sbjct: 5   TLGVLFLIHFFLLSSSAFSKHVSNGIHWWCTKTTYPETCKYFYGHANNPPPKDMSEFKKM 64

Query: 64  VIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCTEFD 123
            I+FAM+ A SVE H K VGS+CRN KER AWADCLKLYQNTIFQL  TLDSS K TEFD
Sbjct: 65  AIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLERTLDSSAKSTEFD 124

Query: 124 IQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKETYK 183
           IQTWLSTALTN+ETCRTGFAELNV D VLPLITSNNVT+LISNSLAVNNASTW GKETYK
Sbjct: 125 IQTWLSTALTNVETCRTGFAELNVVDNVLPLITSNNVTQLISNSLAVNNASTWQGKETYK 184

Query: 184 KGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRG 243
           +GFPSWLSGGDRKLLQSSS+  DLVVAQDGSGDHKTVAAALEEAAKR+TDGRFVIQVKRG
Sbjct: 185 QGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRRTDGRFVIQVKRG 244

Query: 244 VYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGITFR 303
           VYEENL+IGNKMKNIMLVGDGMRFTFITG+RSVGGGSTTFNSATVAVTGEGF+A+GITFR
Sbjct: 245 VYEENLEIGNKMKNIMLVGDGMRFTFITGSRSVGGGSTTFNSATVAVTGEGFIAQGITFR 304

Query: 304 NTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 363
           NTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA
Sbjct: 305 NTAGPENHQAVALRSGADRSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAA 364

Query: 364 VVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLG 423
           VVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK VESTVKT+LG
Sbjct: 365 VVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTFLG 424

Query: 424 RPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKWKGY 483
           RPWKEYSRTVF+KTYLD+LVDPAGWLEWNGDFALNTLYYGEYKNIGP SST QRV+WKGY
Sbjct: 425 RPWKEYSRTVFIKTYLDTLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGY 484

Query: 484 RVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
            VISNSTEASEF+VQNFI+GQSWLPGTEVPFIPG+
Sbjct: 485 HVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 519

BLAST of Tan0012794 vs. ExPASy TrEMBL
Match: A0A5D3DJL7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440 PE=3 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.3e-268
Identity = 460/521 (88.29%), Postives = 482/521 (92.51%), Query Frame = 0

Query: 1   MEKS-IGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSE 60
           ME S +GV   L FFLLSSSAFS   SNGIDWWC+KTTYPETCKYFF+HG +SPPKDM++
Sbjct: 6   METSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTD 65

Query: 61  FKKMVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKC 120
           FKKMVIQFAME+A S ESH KGVGSKCRN KE+AAWADCLKLYQNTI QLN TLDSSTKC
Sbjct: 66  FKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKC 125

Query: 121 TEFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNAS--TWP 180
           TEFDIQTWLSTALTNLETCRTGFAELNV DY+ PLI S+NVTKLISNSLA+NNAS     
Sbjct: 126 TEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGN 185

Query: 181 GKETYKKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFV 240
           GK TYKKGFPSWLSGGDR+LLQS S K DLVVAQDGSG++ TV AALEEAAKRKT GRFV
Sbjct: 186 GKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFV 245

Query: 241 IQVKRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMA 300
           IQVKRGVY ENL+IG+KMKNIML+GDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGF+A
Sbjct: 246 IQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIA 305

Query: 301 RGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF 360
           RGITF+NTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF
Sbjct: 306 RGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF 365

Query: 361 IFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 420
           IFGNAAVVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST
Sbjct: 366 IFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 425

Query: 421 VKTYLGRPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQR 480
           VKTYLGRPWKEYSRTVFMKTY+DSLVDP GWLEW+GDFALNTLYYGEY NIGPGS  SQR
Sbjct: 426 VKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQR 485

Query: 481 VKWKGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           VKWKGY VISNSTEASEFTVQNFIAGQSWLP TEVPF PGL
Sbjct: 486 VKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

BLAST of Tan0012794 vs. ExPASy TrEMBL
Match: A0A0A0L9G4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G175720 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.3e-268
Identity = 461/521 (88.48%), Postives = 483/521 (92.71%), Query Frame = 0

Query: 1   MEKSIGVLFLLQFFLL-SSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSE 60
           M  S GV   L FFLL SSSAFS   SNGIDWWC+KTTYPETCKYFF+HG +SPPKDM++
Sbjct: 51  MGTSFGVFIFLHFFLLSSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTD 110

Query: 61  FKKMVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKC 120
           FKKMVIQFAME+A S ESH KGVGSKCRN KE+AAWADCLKLYQNTI QLN TLDSSTK 
Sbjct: 111 FKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKS 170

Query: 121 TEFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNAS--TWP 180
           TEFDIQTWLSTALTNLETCRTGFAELNVSDY+LPLI S+NVT+LISNSLA+NNAS     
Sbjct: 171 TEFDIQTWLSTALTNLETCRTGFAELNVSDYILPLIMSDNVTELISNSLAINNASAGVGN 230

Query: 181 GKETYKKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFV 240
           GKETYKKGFPSWLSGGDR+LLQSSS K DLVVAQDGSG++ TV AALEEAAKRKT GRFV
Sbjct: 231 GKETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFV 290

Query: 241 IQVKRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMA 300
           IQVKRGVY ENL+IG+KMKNIML+GDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGF+A
Sbjct: 291 IQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIA 350

Query: 301 RGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF 360
           RGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF
Sbjct: 351 RGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF 410

Query: 361 IFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 420
           IFGNAAVVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST
Sbjct: 411 IFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 470

Query: 421 VKTYLGRPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQR 480
           VKTYLGRPWKEYSRTVFMKTY+DSLVDPAGWLEW+GDFALNTLYYGEY NIGPGS  SQR
Sbjct: 471 VKTYLGRPWKEYSRTVFMKTYIDSLVDPAGWLEWDGDFALNTLYYGEYNNIGPGSPISQR 530

Query: 481 VKWKGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           VKWKGY VI+N TEASEFTVQNFIAGQSWLP TEVPF PGL
Sbjct: 531 VKWKGYHVITNLTEASEFTVQNFIAGQSWLPDTEVPFTPGL 571

BLAST of Tan0012794 vs. ExPASy TrEMBL
Match: A0A6J1JEY1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111486289 PE=3 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.2e-267
Identity = 459/519 (88.44%), Postives = 481/519 (92.68%), Query Frame = 0

Query: 1   MEKSI-GVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPPKDMSE 60
           ME+SI GVLF L FFL SSSAFS H SNGI WWC KTTYPETCKYF+ +    PPKDMSE
Sbjct: 32  MERSILGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANNPPPKDMSE 91

Query: 61  FKKMVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKC 120
           FKKM I+FAM+ A SVE H K VGS+CRN KER AWADCLKLYQNTIFQL  TL+SSTK 
Sbjct: 92  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKS 151

Query: 121 TEFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGK 180
           TEFDIQTWLSTALTN+ETCRTGFAELNV D VLPLITS NVTKLISNSLAVNNASTW GK
Sbjct: 152 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGK 211

Query: 181 ETYKKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQ 240
           ETYK+GFPSWLSGGDRKLLQSSS+  DLVVAQDGSGDHKTVAAALEEAAKR TDGRFVIQ
Sbjct: 212 ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQ 271

Query: 241 VKRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARG 300
           VKRGVYEENL+IGNKMKNIMLVGDGMRFTFIT +RSVGGGSTTFNSATVAVTGEGF+A+G
Sbjct: 272 VKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQG 331

Query: 301 ITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 360
           ITFRNTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF
Sbjct: 332 ITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 391

Query: 361 GNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVK 420
           GNAAVVLQNCMIYAR+PMNGQKNA+TAQGRTDPNQNTGISIHNSRVMATDDLK VE TVK
Sbjct: 392 GNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVK 451

Query: 421 TYLGRPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVK 480
           T+LGRPWKEYSRTVF+KTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGP SST QRV+
Sbjct: 452 TFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ 511

Query: 481 WKGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           WKGY VISNSTEASEF+VQNFI+GQSWLPGTEVPFIPG+
Sbjct: 512 WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 550

BLAST of Tan0012794 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 599.4 bits (1544), Expect = 2.8e-171
Identity = 300/508 (59.06%), Postives = 386/508 (75.98%), Query Frame = 0

Query: 15  LLSSSAFSNHVSNGIDWWCRKTTYPETCKYFFSHGRRSPP-KDMSEFKKMVIQFAMEKAS 74
           +L +S  S +    +  WC +T  P+ C+YF +H   + P K  SEF K+ ++  +++A 
Sbjct: 16  ILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAI 75

Query: 75  SVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLDSSTKCTEFDIQTWLSTALT 134
             ++HA  +G KCR+ +E+AAW DC+KLY  T+ ++N T+D + KC++ D QTWLSTALT
Sbjct: 76  LAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALT 135

Query: 135 NLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNAS---TWPGKETYKKGFPSWL 194
           NL+TCR GF EL V+D VLPL+ SNNV+ L+ N+LA+N      T P K+    GFPSW+
Sbjct: 136 NLDTCRAGFLELGVTDIVLPLM-SNNVSNLLCNTLAINKVPFNYTPPEKD----GFPSWV 195

Query: 195 SGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRGVYEENLD 254
             GDRKLLQSS+ K + VVA+DGSG+ KT+  A++ A+     GRFVI VK+GVY ENL+
Sbjct: 196 KPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYSENLE 255

Query: 255 IGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGITFRNTAGPEN 314
           I  + KN+ML GDG+  T ITG++SVGGG+TTFNSATVA  G+GF+ARGITFRNTAG  N
Sbjct: 256 I--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASN 315

Query: 315 HQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCM 374
            QAVALRSG+DLSVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA VLQNC 
Sbjct: 316 EQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCN 375

Query: 375 IYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYS 434
           I+ARRP   + N +TAQGR+DPNQNTGI IHNSRV A  DL+PV  + KTYLGRPW++YS
Sbjct: 376 IFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYS 435

Query: 435 RTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPGSSTSQRVKWKGYRVISNST 494
           RTVFMKT LDSL+DP GWLEW+G+FAL TL+Y E++N GPG+STS RV W G+RV+ +++
Sbjct: 436 RTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSAS 495

Query: 495 EASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           EAS+FTV  F+AG SW+P + VPF  GL
Sbjct: 496 EASKFTVGTFLAGGSWIP-SSVPFTSGL 511

BLAST of Tan0012794 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 569.3 bits (1466), Expect = 3.1e-162
Identity = 290/524 (55.34%), Postives = 370/524 (70.61%), Query Frame = 0

Query: 4   SIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCK-YFFSHGRRSPPKDMSEFKK 63
           S+ +L +L   L   +  ++  S  ID WC KT YP  CK YF  H     P  +SEF+ 
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRV 72

Query: 64  MVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTLD-----SST 123
           ++++ AM++A S           C + K++A  ADC+ LY +T+ QLN TL      +  
Sbjct: 73  LLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGR 132

Query: 124 KCTEFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWP 183
           +CT+FD QTWLSTALTN ETCR G ++LNVSD+  P++++  ++ LISN LAVN A    
Sbjct: 133 RCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTA 192

Query: 184 GKETY----KKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRK-T 243
           GK        KGFP+W+S  +R+LLQ  SV+A+LVVA+DGSG  KTV AA++ A +RK T
Sbjct: 193 GKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVT 252

Query: 244 DGRFVIQVKRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 303
            GRFVI VKRG+Y+ENL++     NIMLVGDGMR+T ITG RSV GG TT++SAT  + G
Sbjct: 253 SGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEG 312

Query: 304 EGFMARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 363
             F+A+GI F+NTAGP   QAVALRS +DLS+FYRC+ EGYQDTL VHSQRQFY+ECYIY
Sbjct: 313 LHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIY 372

Query: 364 GTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 423
           GTVDFIFGNAAVV QNC+I  R P+ GQ N +TAQGRTD  QNTGISIHNS ++   DLK
Sbjct: 373 GTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLK 432

Query: 424 PVESTVKTYLGRPWKEYSRTVFMKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGP 483
           PV  +VKTY+GRPW  YSRTV +KTY+DS+V P GW  W     + L+TL+Y EYKNIGP
Sbjct: 433 PVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGP 492

Query: 484 GSSTSQRVKWKGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPF 515
            SST  RV+WKG+ V+S +++AS F+V  FIAG +WLPG+ +PF
Sbjct: 493 ASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

BLAST of Tan0012794 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 568.2 bits (1463), Expect = 7.0e-162
Identity = 290/530 (54.72%), Postives = 370/530 (69.81%), Query Frame = 0

Query: 4   SIGVLFLLQFFLLSSSAFSNHVSNGIDWWCRKTTYPETCK-YFFSHGRRSPPKDMSEFKK 63
           SI  L L+   LL     +         WC KT YP+ CK YF +H     P  +SEF+ 
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRV 68

Query: 64  MVIQFAMEKASSVESHAKGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTL-------DS 123
           M+++ AM++A S  +     G  C + K++A  ADC+ LY +TI QLN TL        +
Sbjct: 69  MLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGA 128

Query: 124 STKCTEFDIQTWLSTALTNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNAST 183
           +  CT+FD QTWLSTALTN ETCR G ++LNV+D++ P++++  ++ LISN LAVN A  
Sbjct: 129 AKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALL 188

Query: 184 WPGKE----TYKKGFPSWLSGGDRKLLQSSSVKADLVVAQDGSGDHKTVAAALEEAAKRK 243
             G +      +KGFP+WLS  D++LL+  +V+A+LVVA+DGSG   TV AA++ A +RK
Sbjct: 189 TAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAGRRK 248

Query: 244 -TDGRFVIQVKRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAV 303
            T GRFVI VKRG+Y+EN+++     +IMLVGDGMR T ITG RSV GG TT+NSAT  +
Sbjct: 249 VTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGI 308

Query: 304 TGEGFMARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECY 363
            G  F+A+GITFRNTAGP   QAVALRS +DLS+FY+C+ EGYQDTL VHSQRQFY+ECY
Sbjct: 309 EGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECY 368

Query: 364 IYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDD 423
           IYGTVDFIFGNAA V QNC+I  RRP+ GQ N +TAQGR DP QNTGISIHNSR++   D
Sbjct: 369 IYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPD 428

Query: 424 LKPVESTVKTYLGRPWKEYSRTVFMKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNI 483
           LKPV  TVKTY+GRPW ++SRTV ++TYLD++V P GW  W     F L+TL+Y EYKN 
Sbjct: 429 LKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNT 488

Query: 484 GPGSSTSQRVKWKGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           GP SST  RV WKG+ V+  +++AS FTV  FIAG +WLP T +PF  GL
Sbjct: 489 GPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Tan0012794 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 553.1 bits (1424), Expect = 2.3e-157
Identity = 280/515 (54.37%), Postives = 358/515 (69.51%), Query Frame = 0

Query: 25  VSNGIDWWCRKTTYPETCKYFFSHGRRS-----PPKDMSEFKKMVIQFAMEKASSVESHA 84
           VS  + WWC KT + ETC Y+F    ++     PP+  SEF +M+++ A+++A    S  
Sbjct: 80  VSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQT 139

Query: 85  KGVGSKCRNEKERAAWADCLKLYQNTIFQLNHTL-------DSSTKCTEFDIQTWLSTAL 144
              G  C N + +AAW+DC+ L+QNT+ QLN TL        S  KCT+FD QTWLSTA 
Sbjct: 140 VKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQ 199

Query: 145 TNLETCRTGFAELNVSDYVLPLITSNNVTKLISNSLAVNNASTWPGKET----YKKGFPS 204
           TN+ETCR+G  +LNVSD+V+P+I++ N++ LI N LAVN         T    +K+ FPS
Sbjct: 200 TNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPS 259

Query: 205 WLSGGDRKLLQSSSV---KADLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQVKRGVY 264
           W+S  +R+LL S+S+      LVVAQD SG  +++ AA+  AA+R+   RFVI VK+GVY
Sbjct: 260 WVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVY 319

Query: 265 EENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFMARGITFRNT 324
            EN+D+GN   NIMLVGDG R T IT  RSV  G TT+NSAT    G+ F+A+ +TF NT
Sbjct: 320 RENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINT 379

Query: 325 AGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVV 384
           AGP   QAVA+RS +DLSVFYR    G+QDTLY+HSQRQF++ECYI GT+DFIFGNAAVV
Sbjct: 380 AGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVV 439

Query: 385 LQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRP 444
            QNCMI  RRP++GQ N +TAQGR DP QNTGI+IH+SR++A  DLKPV    KTYLGRP
Sbjct: 440 FQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRP 499

Query: 445 WKEYSRTVFMKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPGSSTSQRVKWKGY 504
           W+ YSR   MKTY+D+ + P GW  W    +FALNT++YGEYKN GPGSST  RV+WKG+
Sbjct: 500 WQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGF 559

Query: 505 RVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
             I+++  AS FTV + IAG SWLP T VPF  GL
Sbjct: 560 HAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Tan0012794 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 440.7 bits (1132), Expect = 1.7e-123
Identity = 252/532 (47.37%), Postives = 326/532 (61.28%), Query Frame = 0

Query: 33  CRKTTYPETCKYFFSHGRRSPPKDMSE---FKKMVIQFAMEKA-------SSVESHAKGV 92
           C++T YP  C +  S+       D ++   F  +V+   M++A       SS++ H    
Sbjct: 38  CKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQH---- 97

Query: 93  GSKCRNEKERAAWADCLKLYQNTIFQLNHTLDS-STKCTEFDIQTWLSTALTNLETCRTG 152
                ++   +A  DCL+LY++TI QLNH+  S     +  D QT LS A+ N +TCR G
Sbjct: 98  --HSLHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNG 157

Query: 153 FAELNV-SDY--VLPLITSNNVTKLISNSLAVNNAS---------------TWPGKETYK 212
           F +  + S Y    P+    N+TK ISNSLAV  A+               T   K+   
Sbjct: 158 FRDFKLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSS 217

Query: 213 KG--------------FPSWLSGGDRKLLQSS--SVKADLVVAQDGSGDHKTVAAALEEA 272
            G              FPSW    DRKLL+ S  + KADLVVA+DGSG + ++  A+  A
Sbjct: 218 AGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAA 277

Query: 273 AK-RKTDGRFVIQVKRGVYEENLDIGNKMKNIMLVGDGMRFTFITGNRSVGGGSTTFNSA 332
           AK  + + R VI VK GVY EN+ I   +KN+M++GDG+  T +TGNR+V  G+TTF SA
Sbjct: 278 AKLPRRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSA 337

Query: 333 TVAVTGEGFMARGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFY 392
           T AV+G GF+A+GITF NTAGPE HQAVALRS +D SVFY C+F+GYQDTLY+HS RQF 
Sbjct: 338 TFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFL 397

Query: 393 KECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVM 452
           + C IYGTVDFIFG+A  +LQNC IYAR+PM+GQKN +TAQ R +P++ TG  I +S V 
Sbjct: 398 RNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVA 457

Query: 453 ATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYLDSLVDPAGWLEWNGDFALNTLYYGEYK 512
              +         TYLGRPW+ +SRTVFMK  L +LV PAGWL W+G FAL+TLYYGEY 
Sbjct: 458 TASE---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYG 517

Query: 513 NIGPGSSTSQRVKWKGYRVISNSTEASEFTVQNFIAGQSWLPGTEVPFIPGL 519
           N G G+S S RVKW GY VI   TEA +FTV+NF+ G  W+  T VP   GL
Sbjct: 518 NTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554