Tan0012136 (gene) Snake gourd v1

Overview
NameTan0012136
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionacidic mammalian chitinase-like
LocationLG08: 2840101 .. 2841789 (+)
RNA-Seq ExpressionTan0012136
SyntenyTan0012136
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCAAATCACAAAGTTTGTGAATGCTGGTTACTGGCTTGCTAGTACGGATGAGACACTCCCAGTTGCAAATATCGACGCAACACTTTTTAGCCATTTGTTTGTTGGTTATGTTGGTGTCAACTCCCAAACTTATGAAGTCACTGTAGATCCTTCACAAGCTATCCTAATCAATCAGTTTTCTTCAACTGTGAGACTACGTAATCCTGGGATCAAAACTCTGTTGTCCATTGCAGGAAGCAGTGATGTATTTGCTAAAATGGCCGAGGAGCCTGCAAATCGGGATGCCTTTATTAAATCATCAATTGAGGTAGCCAGAGCAGGTGAATTTGATGGCCTCGATCTTCAATGGTTATACCCATCTTCTCACGATGAAATGAACAATTTTGAATCTCTTCTTAGTTTTTGGCATAAAGCCATTGTGGATGATGCTATCAATTTGAGCCTCCCACAGTTGCTCCTTGTTGCCACAGTCTCCAACGTACCATATCTTCAACACAACATTCAATATCCTGTTGATGCAATCAAACAAAACTTGGATTGGGTGAACGTTCTTTCTTATGACTTTTACACCCCAACTTCCTCAAGCAAGTCTACAGGACCATCATCAGCATTCCACAATCCGAAGGTCAATGCTCTATCTGCACATTTTGGTATTCAAACTTGGATGCAAAGCCTTCATAATCTACCTCATAAGAAAATTGTATTCGGAATTCCATTCCATGGCTGGGCTTGGAAACTCGCCAACTCTCACCAACACGACATCTTTTCTAAAGCCGAAGGAGTAGCCACAGGAGAGTATATTTCTCCAAATGGCCAAATCTACTACTCCAAAGTGAAACAGTTTATAGATGATAATCAAGCTGGAAATGAGAACAAGGACCCTAAGTATTTAATTGCCTACACTTATTCTGGCACAACCTGGATTGCGTACGAAAGTGAATATACCATTGCAGCCAAGATTTCTTTGGCAAAGTCTGACAATGTGCTCGGTTACTTTGCATTCAACATTGCAGCCGATGATGTTGTTAACACTCTCGCTAATGCTGCCAAATCCAGTTGGTGATATATTTCAACATCGTCCTTCACACTGCGCCGAACACTTCATTTTCTTATCTTATATTGAAGCATCCAATAAAACGCATGCTAGCTCATGCTTGTGTGTCTTTCTAAACTAGGTTGATGGCCGTGGTGTTTGTTGTCTTTCTTATTTCAAATTTCCTTTTTTATTATCTTCTATCTTGTGTGGTGTTCAAAATTTGAAGTTTTATTTTTAAAATAAAATTACTGGTGTGTTTTTATCAACTTCTCTAATTTTCTCCACAAATTTCTAAGAGTATTCCATTGGCTAAGCTCGAAATATTTAATTCCCCCTCTTCATATTATTAGTGACGTTGCTAATTTAACATTGATTAATTTAATGGGCTTCTAACCCATTAAAATTCATATGAGAGATTAGTAGAGCAAATATGTCTTTTATCTCCAAACAATAATTTTTTCGCATACTTGAAACTACACATCTTATAATAGAAATATTATTTGATGAGGCTCAATGTGATAATCAATAGACCATTTAAGAACTAAATTTTAACCATGTATTGAATTGTTATATATTAAAAATCAATAATCACAGTTAAAGAAGGCTTGGAGTTCATCGGAGAGGTCTTGGAAACCTTTTTCCTTTTAG

mRNA sequence

ATGGCTCAAATCACAAAGTTTGTGAATGCTGGTTACTGGCTTGCTAGTACGGATGAGACACTCCCAGTTGCAAATATCGACGCAACACTTTTTAGCCATTTGTTTGTTGGTTATGTTGGTGTCAACTCCCAAACTTATGAAGTCACTGTAGATCCTTCACAAGCTATCCTAATCAATCAGTTTTCTTCAACTGTGAGACTACGTAATCCTGGGATCAAAACTCTGTTGTCCATTGCAGGAAGCAGTGATGTATTTGCTAAAATGGCCGAGGAGCCTGCAAATCGGGATGCCTTTATTAAATCATCAATTGAGGTAGCCAGAGCAGGTGAATTTGATGGCCTCGATCTTCAATGGTTATACCCATCTTCTCACGATGAAATGAACAATTTTGAATCTCTTCTTAGTTTTTGGCATAAAGCCATTGTGGATGATGCTATCAATTTGAGCCTCCCACAGTTGCTCCTTGTTGCCACAGTCTCCAACGTACCATATCTTCAACACAACATTCAATATCCTGTTGATGCAATCAAACAAAACTTGGATTGGGTGAACGTTCTTTCTTATGACTTTTACACCCCAACTTCCTCAAGCAAGTCTACAGGACCATCATCAGCATTCCACAATCCGAAGAAAATTGTATTCGGAATTCCATTCCATGGCTGGGCTTGGAAACTCGCCAACTCTCACCAACACGACATCTTTTCTAAAGCCGAAGGAGTAGCCACAGGAGAGTATATTTCTCCAAATGGCCAAATCTACTACTCCAAAGTGAAACAGTTTATAGATGATAATCAAGCTGGAAATGAGAACAAGGACCCTAAGTATTTAATTGCCTACACTTATTCTGGCACAACCTGGATTGCGTACGAAAGTGAATATACCATTGCAGCCAAGATTTCTTTGGCAAAGTCTGACAATGTGCTCGGTTACTTTGCATTCAACATTGCAGCCGATGATGTTAAGGCTTGGAGTTCATCGGAGAGGTCTTGGAAACCTTTTTCCTTTTAG

Coding sequence (CDS)

ATGGCTCAAATCACAAAGTTTGTGAATGCTGGTTACTGGCTTGCTAGTACGGATGAGACACTCCCAGTTGCAAATATCGACGCAACACTTTTTAGCCATTTGTTTGTTGGTTATGTTGGTGTCAACTCCCAAACTTATGAAGTCACTGTAGATCCTTCACAAGCTATCCTAATCAATCAGTTTTCTTCAACTGTGAGACTACGTAATCCTGGGATCAAAACTCTGTTGTCCATTGCAGGAAGCAGTGATGTATTTGCTAAAATGGCCGAGGAGCCTGCAAATCGGGATGCCTTTATTAAATCATCAATTGAGGTAGCCAGAGCAGGTGAATTTGATGGCCTCGATCTTCAATGGTTATACCCATCTTCTCACGATGAAATGAACAATTTTGAATCTCTTCTTAGTTTTTGGCATAAAGCCATTGTGGATGATGCTATCAATTTGAGCCTCCCACAGTTGCTCCTTGTTGCCACAGTCTCCAACGTACCATATCTTCAACACAACATTCAATATCCTGTTGATGCAATCAAACAAAACTTGGATTGGGTGAACGTTCTTTCTTATGACTTTTACACCCCAACTTCCTCAAGCAAGTCTACAGGACCATCATCAGCATTCCACAATCCGAAGAAAATTGTATTCGGAATTCCATTCCATGGCTGGGCTTGGAAACTCGCCAACTCTCACCAACACGACATCTTTTCTAAAGCCGAAGGAGTAGCCACAGGAGAGTATATTTCTCCAAATGGCCAAATCTACTACTCCAAAGTGAAACAGTTTATAGATGATAATCAAGCTGGAAATGAGAACAAGGACCCTAAGTATTTAATTGCCTACACTTATTCTGGCACAACCTGGATTGCGTACGAAAGTGAATATACCATTGCAGCCAAGATTTCTTTGGCAAAGTCTGACAATGTGCTCGGTTACTTTGCATTCAACATTGCAGCCGATGATGTTAAGGCTTGGAGTTCATCGGAGAGGTCTTGGAAACCTTTTTCCTTTTAG

Protein sequence

MAQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQFSSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYPSSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLDWVNVLSYDFYTPTSSSKSTGPSSAFHNPKKIVFGIPFHGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIAYTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADDVKAWSSSERSWKPFSF
Homology
BLAST of Tan0012136 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.0e-63
Identity = 139/350 (39.71%), Postives = 201/350 (57.43%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQFSSTVRL 67
           V A YW  +++   PV +ID++LF+HLF  +  +NSQT +VTV  +     + F+ TV+ 
Sbjct: 28  VKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 68  RNPGIKTLLSIAG---SSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYPSSH 127
           RNP +KTLLSI G       +A MA  P +R +FI SSI VAR+  F GLDL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 128 DEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATV--SNVPYLQHNIQYPVDAIKQNLDW 187
            EM NF +LL  W  A+V +A +   P+LLL A V  SN  Y   ++ YPV A+  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 188 VNVLSYDFYTPTSSSKSTGPSSAFHNP---------------------KKIVFGIPFHGW 247
           VN+++YDFY P   S+ TGP +A  +P                     KK V G P++G+
Sbjct: 208 VNLMAYDFYGP-GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFPYYGY 267

Query: 248 AWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIAYTY 307
           AW+L N++ H  ++   G A    ISP+G I Y ++++FI DN A     +   +  Y Y
Sbjct: 268 AWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGA-TTVYNSTVVGDYCY 327

Query: 308 SGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADDVKAWS-SSERSW 331
           +GT WI Y+   +I  K+  AK   +LGYF++++ ADD    S ++ ++W
Sbjct: 328 AGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDNSGLSRAASQAW 366

BLAST of Tan0012136 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.7e-47
Identity = 117/348 (33.62%), Postives = 184/348 (52.87%), Query Frame = 0

Query: 12  YWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQFSSTVRLRNPG 71
           YWL   +   P   +D +LF+H+F  ++  N+ TY++ +  SQ + +N F+ T + ++P 
Sbjct: 39  YWL--ENPFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKTKSPP 98

Query: 72  IKTLLSIAG---SSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYPSSHDEMN 131
             TL SI G   +S + A +A +P    AFI S+I+VAR   FDG+DL W +P +  EMN
Sbjct: 99  AATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNTKEMN 158

Query: 132 NFESLLSFWHKAIVDDAINLSLPQLLLVATV---SNVPYLQHNIQYPVDAIKQNLDWVNV 191
           +   +L  W KAI D+      P LLL A V    N         YPV++I +NLDWVNV
Sbjct: 159 DLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLDWVNV 218

Query: 192 LSYDFYTPTSSSKSTGPSSAF----------------HN---PKKIVFGIPFHGWAWKLA 251
           +SY+   P  S+K+  PS  F                H+   P+K+V G+P +G +WKL 
Sbjct: 219 MSYELRGP-RSNKTGAPSGTFDPKSNVSVVSGLLSWIHSGVVPEKLVMGMPLYGKSWKLR 278

Query: 252 NSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIAYTYSGTTW 311
           + + H I + + G   G     NG + Y +V  F +  ++     D      Y+YSG+TW
Sbjct: 279 DPNVHGIGAPSVGSGPG----VNGLMAYFQVLDF-NRQKSAKVEYDVDTASVYSYSGSTW 338

Query: 312 IAYESEYTIAAKISLAKSDNVLGYFAFNIAADDVKAW---SSSERSWK 332
           I Y++ +T++ K+  A++  + GYF F +A  D   W   + + ++WK
Sbjct: 339 IGYDNPFTVSIKVGFAQALKLRGYF-FWVAGLDTLDWKIATQASKAWK 377

BLAST of Tan0012136 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 8.9e-46
Identity = 122/361 (33.80%), Postives = 186/361 (51.52%), Query Frame = 0

Query: 5   TKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQ----AILINQ 64
           T  V   YW+   +   P ++ID +LF+H+F  +V  N  TY++  +       A  +  
Sbjct: 34  TTRVKGIYWI--ENPLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTT 93

Query: 65  FSSTVRLRNPGIKTLLSIAG---SSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQ 124
           F++T + + P I TLLSI G   +S +FA +A +P  R  FI S+I+VAR   FDG+D  
Sbjct: 94  FTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFD 153

Query: 125 WLYPSSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATV---SNVPYLQHNIQYPVD 184
           W +P++  EMN+   LL  W +AI D+  + S P LLL A V    N         YPVD
Sbjct: 154 WEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVD 213

Query: 185 AIKQNLDWVNVLSYDFYTPTSSSKSTGPSSAFHN-------------------PKKIVFG 244
           +I +NLDWVNV+SYD    + S+ +  PS  F +                   P+KIV G
Sbjct: 214 SINKNLDWVNVMSYDL-RGSGSNVTGAPSGMFDSKSNVSVVSGLFSWIRGGVAPEKIVMG 273

Query: 245 IPFHGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKY 304
           +P +G +WKL + + H I +   G   G     +G + Y +V  F +         D + 
Sbjct: 274 MPLYGKSWKLQDPNVHGIGAPNVGPGPG----VDGGMAYFQVVDF-NKQMGAKVVYDKET 333

Query: 305 LIAYTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADDVKAW--SSSERSWKPF 335
              Y+YSG+TWI Y+  +T++ K+  A++  + GYF F  A  D   W  S++ ++W+P 
Sbjct: 334 GSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYF-FWAAGYDTSDWKVSTASKAWRPE 385

BLAST of Tan0012136 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 1.5e-37
Identity = 107/349 (30.66%), Postives = 181/349 (51.86%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQFSSTVRL 67
           V A YW A +D     ++ID   F+H++  +V    +++ + +  S      ++     +
Sbjct: 55  VRAAYWPAWSD--FSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKYDGIHNI 114

Query: 68  RNPGIKTLLSIAG---SSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYPSSH 127
           R P + TLLSI G   ++ +F++MA    NR +FI S+I VAR  EF+GLDL W +P   
Sbjct: 115 R-PRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDE 174

Query: 128 DEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATV---SNVPYLQHNIQ-YPVDAIKQNL 187
            +M+N   LL  W+KA+V +A      +LLL + V   S +  + +  + YPV AI++ L
Sbjct: 175 KDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYL 234

Query: 188 DWVNVLSYDFYTPTSSSKSTGPSSAFHNP--------------------KKIVFGIPFHG 247
           DW + + +D+    + +  TG ++A ++P                    +K+V G+P +G
Sbjct: 235 DWASPMCFDY--NGAWANETGFNAALYDPNSNISTKYGIGSWIGSGVPAEKLVMGLPLYG 294

Query: 248 WAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIAYT 307
            AW+L + + H + +KA G A    +  +G + Y ++  F  D  A     D   +  Y+
Sbjct: 295 RAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVFNKDTGA-KVVYDEVAVSFYS 354

Query: 308 YSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADDVKAWSSSERS 330
           YSGTTWI Y+   +I  K+  A+S  + GYF + I  D  K W+ S+++
Sbjct: 355 YSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKD--KDWTISKQA 391

BLAST of Tan0012136 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.9e-35
Identity = 103/348 (29.60%), Postives = 175/348 (50.29%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQFSSTVRL 67
           V + YW A  D     + ID   F+H+ + ++     ++++ +  S       F   +R 
Sbjct: 40  VRSAYWPAGDD--FSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 68  RNPGIKTLLSIAG---SSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYPSSH 127
           R+P +KTLLSI G   +S +F+++A    NR+ FI S+IEVAR   FDG+DL W +P + 
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 128 DEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATV---SNVPYL-QHNIQ-YPVDAIKQN 187
            +M N   L   W+ A+  +A     P+LLL + V   S V  + +H  + YP  AI + 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKY 219

Query: 188 LDWVNVLSYDFY-----------TPTSSSKSTGPSSAFHN-------PKKIVFGIPFHGW 247
           LDW + + +D++               S      +   H+       P+K+V G+  +G 
Sbjct: 220 LDWASPMCFDYHGTWDNNTDFNAALYDSKSEISTNFGLHSWIKSGVRPEKLVMGLALYGR 279

Query: 248 AWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIAYTY 307
           AW+L + + + + ++A G AT      +G + Y+++ +F +     N   D   +  Y+Y
Sbjct: 280 AWELKDPNVNGVGAEAVGPAT----DTDGSMNYNEILKF-NKQSGANVVYDKVAISFYSY 339

Query: 308 SGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADDVKAWSSSERS 330
           +GTTWI Y+   +I  K+  AKS  + GYF + +  D  K WS S+++
Sbjct: 340 AGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKD--KDWSISKQA 378

BLAST of Tan0012136 vs. NCBI nr
Match: XP_022999580.1 (chitotriosidase-1-like [Cucurbita maxima])

HSP 1 Score: 491.1 bits (1263), Expect = 7.5e-135
Identity = 239/341 (70.09%), Postives = 276/341 (80.94%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQF 61
           A +T+FVNAGYWLASTD TLP ANIDATLF+HLF GY  +NS TY+VTVDPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYP 121
           SSTV+L NPG+KTLLSIAG + VFA MA +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLD 181
           SS D+M NF++L+  WH+A+VDDA ++SLPQLLLVATVSN+PYLQ+NIQYP DAI QNLD
Sbjct: 125 SSQDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLD 184

Query: 182 WVNVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPF 241
           WVN+LSYDFYTP S   +TGPSSAFHNP                       KKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIA 301
           HGWAWKLANSHQH +F+KA+G A+GEYI P+GQI+YS V++FI+DNQAG E+KD  YL+A
Sbjct: 245 HGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           Y Y  TTWI +ESEYT+A KI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0012136 vs. NCBI nr
Match: XP_022946077.1 (acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mammalian chitinase-like [Cucurbita moschata])

HSP 1 Score: 487.6 bits (1254), Expect = 8.3e-134
Identity = 239/341 (70.09%), Postives = 273/341 (80.06%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQF 61
           A +T+FVNAGYWLASTD+ LP ANIDATLF+HLF GY  +NS TY+VTVDPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYP 121
           SSTV+L NP +KTLLSIA  + VFA MA +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLD 181
           SS D+M+NF++L+  WH+A+VDDA +LSLPQLLLVATVSN+PYLQ NIQYP DAI Q LD
Sbjct: 125 SSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLD 184

Query: 182 WVNVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPF 241
           WVN+LSYDFYTP SS  +TGPSSAFHNP                       KKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIA 301
           HGWAWKL NSHQH +F+KA+G ATGEYI P+GQI+YS V++FI+DNQAG E+KD  YL+A
Sbjct: 245 HGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           Y Y  TTWI YESEYTIA KI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADD 345

BLAST of Tan0012136 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 487.6 bits (1254), Expect = 8.3e-134
Identity = 237/341 (69.50%), Postives = 272/341 (79.77%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQF 61
           A +T+FVNAGYWLASTD+ LP ANIDATLF+HLF GY  +NS TY+VT+DPSQ IL+NQF
Sbjct: 11  ANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQF 70

Query: 62  SSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYP 121
           S TVRL NP +KTLLSIA  + VFA MA +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 71  SPTVRLSNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 130

Query: 122 SSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLD 181
           SS +EM NF++L+  WH+A+VDDA +LSLPQLLLVATVSN+PYLQ NIQYP DAI Q LD
Sbjct: 131 SSQEEMGNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLD 190

Query: 182 WVNVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPF 241
           WVN+LSYDFYTP S+  +TGPSSAFHNP                       KKIVFGIPF
Sbjct: 191 WVNLLSYDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 250

Query: 242 HGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIA 301
           HGWAWKL NSHQH +F+KA+G ATGEYI P+GQI+YS V++FI+DNQAG E+KD  YL+A
Sbjct: 251 HGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 310

Query: 302 YTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           YTY  TTWI YES+YTIA KI+LAK+D VLGYFAFNIAADD
Sbjct: 311 YTYFETTWITYESDYTIADKIALAKADKVLGYFAFNIAADD 351

BLAST of Tan0012136 vs. NCBI nr
Match: KAG6599362.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1 Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 486.9 bits (1252), Expect = 1.4e-133
Identity = 237/341 (69.50%), Postives = 273/341 (80.06%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQF 61
           A +T+FVNAGYWLASTD+ LP ANIDATLF+HLF GY  +NS TY+VT+DPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILIDQF 64

Query: 62  SSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYP 121
           SSTV+L NP +KTLLSIA  + +FA MA +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPEVKTLLSIAADAALFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLD 181
           SS D+M+NF++L+  WH+A+VDDA +LSLPQLLLVATVSN+PYLQ NIQYP DAI Q LD
Sbjct: 125 SSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLD 184

Query: 182 WVNVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPF 241
           WVN+LSYDFYTP SS  +TGPSSAFHNP                       KKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIA 301
           HGWAWKL NSHQH +F+KA+G ATGEYI P+GQI+YS V++FI+DNQAG E+KD  YL+A
Sbjct: 245 HGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           Y Y  TTWI YESEYTIA KI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADD 345

BLAST of Tan0012136 vs. NCBI nr
Match: XP_022999577.1 (chitotriosidase-1-like [Cucurbita maxima])

HSP 1 Score: 486.1 bits (1250), Expect = 2.4e-133
Identity = 237/341 (69.50%), Postives = 274/341 (80.35%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQF 61
           A +T+FVNAGYWLASTD TLP ANIDATLF+HLF GY  +NS TY+VTVDPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYP 121
           SSTV+L NP +KTLLSIAG + VFA MA +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLD 181
           SS D+M NF++L+  WH+A+VDDA ++SLPQLLLVATVSN+PYLQ+NIQYP DAI Q LD
Sbjct: 125 SSQDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLD 184

Query: 182 WVNVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPF 241
           WVN+LSYDFYTP S   +TGPSSAFHNP                       KKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIA 301
           HGWAWKLANSHQH +F+KA+G A+GEYI P+GQI+YS V++FI+DNQAG E+KD  YL+A
Sbjct: 245 HGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           Y Y  TTWI +ESEYT+A KI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0012136 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.6e-135
Identity = 239/341 (70.09%), Postives = 276/341 (80.94%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQF 61
           A +T+FVNAGYWLASTD TLP ANIDATLF+HLF GY  +NS TY+VTVDPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYP 121
           SSTV+L NPG+KTLLSIAG + VFA MA +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLD 181
           SS D+M NF++L+  WH+A+VDDA ++SLPQLLLVATVSN+PYLQ+NIQYP DAI QNLD
Sbjct: 125 SSQDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLD 184

Query: 182 WVNVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPF 241
           WVN+LSYDFYTP S   +TGPSSAFHNP                       KKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIA 301
           HGWAWKLANSHQH +F+KA+G A+GEYI P+GQI+YS V++FI+DNQAG E+KD  YL+A
Sbjct: 245 HGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           Y Y  TTWI +ESEYT+A KI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0012136 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 4.0e-134
Identity = 239/341 (70.09%), Postives = 273/341 (80.06%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQF 61
           A +T+FVNAGYWLASTD+ LP ANIDATLF+HLF GY  +NS TY+VTVDPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYP 121
           SSTV+L NP +KTLLSIA  + VFA MA +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLD 181
           SS D+M+NF++L+  WH+A+VDDA +LSLPQLLLVATVSN+PYLQ NIQYP DAI Q LD
Sbjct: 125 SSQDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLD 184

Query: 182 WVNVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPF 241
           WVN+LSYDFYTP SS  +TGPSSAFHNP                       KKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIA 301
           HGWAWKL NSHQH +F+KA+G ATGEYI P+GQI+YS V++FI+DNQAG E+KD  YL+A
Sbjct: 245 HGWAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           Y Y  TTWI YESEYTIA KI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADD 345

BLAST of Tan0012136 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.2e-133
Identity = 237/341 (69.50%), Postives = 274/341 (80.35%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQF 61
           A +T+FVNAGYWLASTD TLP ANIDATLF+HLF GY  +NS TY+VTVDPSQ ILI+QF
Sbjct: 5   ANVTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQF 64

Query: 62  SSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYP 121
           SSTV+L NP +KTLLSIAG + VFA MA +P NRDAFIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLD 181
           SS D+M NF++L+  WH+A+VDDA ++SLPQLLLVATVSN+PYLQ+NIQYP DAI Q LD
Sbjct: 125 SSQDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLD 184

Query: 182 WVNVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPF 241
           WVN+LSYDFYTP S   +TGPSSAFHNP                       KKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIA 301
           HGWAWKLANSHQH +F+KA+G A+GEYI P+GQI+YS V++FI+DNQAG E+KD  YL+A
Sbjct: 245 HGWAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           Y Y  TTWI +ESEYT+A KI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0012136 vs. ExPASy TrEMBL
Match: A0A6J1G4H5 (chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.1e-128
Identity = 232/341 (68.04%), Postives = 267/341 (78.30%), Query Frame = 0

Query: 2   AQITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQF 61
           A +TKFVNAGYWLAST++ L  ANIDATLF+HLFVGY  ++ +T++VTVDPSQ ILINQF
Sbjct: 5   ANVTKFVNAGYWLASTNDRLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQF 64

Query: 62  SSTVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYP 121
           SSTV+L NP +KTLLSIA  + VFA MA  P NRD FIKSSI  AR G FDGLDLQWLYP
Sbjct: 65  SSTVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYP 124

Query: 122 SSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLD 181
           SS  EM NF++L+  WH+A+VDDA  +SLPQLLLVATVSN+PYLQ N++YP DAI Q LD
Sbjct: 125 SSQYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLD 184

Query: 182 WVNVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPF 241
           WVN+LSYDFYTP SS  +TGPSSAFHNP                       KKIVFGIPF
Sbjct: 185 WVNLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPF 244

Query: 242 HGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIA 301
           HGWAWKL NSHQH  F+KA+G A+GEYI P+GQI+YS V++FI+DNQAG E+KD  YL+A
Sbjct: 245 HGWAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVA 304

Query: 302 YTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           Y Y  TTWI YESEYTIA KI+LAK+D VLGYFAFNIAADD
Sbjct: 305 YAYYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADD 345

BLAST of Tan0012136 vs. ExPASy TrEMBL
Match: A0A6J1G4J9 (acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 9.6e-128
Identity = 230/339 (67.85%), Postives = 265/339 (78.17%), Query Frame = 0

Query: 4   ITKFVNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQFSS 63
           + +FVNAGYWLA TD+TL  ANIDATLF+HLFVGY  ++ +T++VTVDPSQ ILINQFSS
Sbjct: 1   MAEFVNAGYWLAGTDDTLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSS 60

Query: 64  TVRLRNPGIKTLLSIAGSSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYPSS 123
           TV+L NP +KTLLSIA  + VFA MA  P NRD FIKSSI  AR G FDGLDLQWLYPSS
Sbjct: 61  TVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSS 120

Query: 124 HDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLDWV 183
             EM NF++L+  WH+A+VDDA  +SLPQLLLVATVSN+PYLQ N++YP DAI Q LDWV
Sbjct: 121 QYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLDWV 180

Query: 184 NVLSYDFYTPTSSSKSTGPSSAFHNP-----------------------KKIVFGIPFHG 243
           N+LSYDFYTP SS  +TGPSSAFHNP                       KKIVFGIPFHG
Sbjct: 181 NLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHG 240

Query: 244 WAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIAYT 303
           WAWKL NSHQH  F+KA+G A+GEYI P+GQI+YS V++FI+DNQAG E+KD  YL+AY 
Sbjct: 241 WAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 300

Query: 304 YSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           Y  TTWI YESEYTIA KI+LAK+D VLGYFAFNIAADD
Sbjct: 301 YYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADD 339

BLAST of Tan0012136 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 242.3 bits (617), Expect = 5.7e-64
Identity = 139/350 (39.71%), Postives = 201/350 (57.43%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQFSSTVRL 67
           V A YW  +++   PV +ID++LF+HLF  +  +NSQT +VTV  +     + F+ TV+ 
Sbjct: 28  VKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQR 87

Query: 68  RNPGIKTLLSIAG---SSDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYPSSH 127
           RNP +KTLLSI G       +A MA  P +R +FI SSI VAR+  F GLDL W YPSS 
Sbjct: 88  RNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSA 147

Query: 128 DEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATV--SNVPYLQHNIQYPVDAIKQNLDW 187
            EM NF +LL  W  A+V +A +   P+LLL A V  SN  Y   ++ YPV A+  +LDW
Sbjct: 148 TEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLDW 207

Query: 188 VNVLSYDFYTPTSSSKSTGPSSAFHNP---------------------KKIVFGIPFHGW 247
           VN+++YDFY P   S+ TGP +A  +P                     KK V G P++G+
Sbjct: 208 VNLMAYDFYGP-GWSRVTGPPAALFDPSNAGPSGDAGTRSWIQAGLPAKKAVLGFPYYGY 267

Query: 248 AWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIAYTY 307
           AW+L N++ H  ++   G A    ISP+G I Y ++++FI DN A     +   +  Y Y
Sbjct: 268 AWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGA-TTVYNSTVVGDYCY 327

Query: 308 SGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADDVKAWS-SSERSW 331
           +GT WI Y+   +I  K+  AK   +LGYF++++ ADD    S ++ ++W
Sbjct: 328 AGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDNSGLSRAASQAW 366

BLAST of Tan0012136 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 224.9 bits (572), Expect = 9.4e-59
Identity = 132/349 (37.82%), Postives = 190/349 (54.44%), Query Frame = 0

Query: 1   MAQITKF------VNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQ 60
           MA  T+F      V A YW+   +   P  NID+T F+HLF  +  V+S T+EVT+  + 
Sbjct: 1   MADQTQFLRTNSIVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAAN 60

Query: 61  AILINQFSSTVRLRNPGIKTLLSIAG---SSDVFAKMAEEPANRDAFIKSSIEVARAGEF 120
           +  ++ F+ TV+ +N  ++TLLSI G      V A MA    NR AFI SSI++AR  +F
Sbjct: 61  SCQVSSFTHTVKDKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDF 120

Query: 121 DGLDLQWLYPSSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQY 180
            GLDL W YPS+  EM NF  L+  W  A+V+++   +   LLL A V   P   +  +Y
Sbjct: 121 YGLDLAWEYPSNDVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPD-YYGEEY 180

Query: 181 PVDAIKQNLDWVNVLSYDFYTPTSSSKSTGPSSAFHNP---------------------- 240
           PV AI  NLD+VN+++YDFY P   S  TGP +A  +P                      
Sbjct: 181 PVQAIADNLDFVNIMAYDFYGP-GWSPVTGPPAALFDPSNPAGRSGDSGLSKWLEAKLPA 240

Query: 241 KKIVFGIPFHGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNE 300
           KK V G  + GWAW L ++  +   +  +G A    IS +G I Y+K++ +I DN A   
Sbjct: 241 KKAVLGFSYCGWAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGAATF 300

Query: 301 NKDPKYLIAYTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAAD 319
           + DP  +  Y Y GTTWI Y+   +I +K+  AK   +LGYF++++ AD
Sbjct: 301 H-DPAVIGFYCYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

BLAST of Tan0012136 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 135/358 (37.71%), Postives = 193/358 (53.91%), Query Frame = 0

Query: 1   MAQITKF------VNAGYWL----ASTDETL----PVANIDATLFSHLFVGYVGVNSQTY 60
           MA  T+F      V A YW     + + E L    P + ID+TLF+HLF  +  V+S T+
Sbjct: 1   MADQTQFLRTNSIVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTH 60

Query: 61  EVTVDPSQAILINQFSSTVRLRNPGIKTLLSIAG---SSDVFAKMAEEPANRDAFIKSSI 120
           EVT+  + +   + F+ TV+ +N  ++TLLSI G      V A MA    NR AFI SSI
Sbjct: 61  EVTISAANSYQFSSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSI 120

Query: 121 EVARAGEFDGLDLQWLYPSSHDEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVP 180
           ++AR  +F GLDL W YPS+  EM NF  LL  W  A+V+++   +   LLL A V   P
Sbjct: 121 DIARKKDFYGLDLAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSP 180

Query: 181 YLQHNIQYPVDAIKQNLDWVNVLSYDFYTPTSSSKSTGPSSAFHN--------------- 240
                ++YPV AI  NLD+VN+++YDFY P  S  +  P++ FH+               
Sbjct: 181 QYD-GVEYPVKAIADNLDFVNIMAYDFYGPGWSPVTGPPAALFHDPSNPAGRSGNSGLRK 240

Query: 241 --------PKKIVFGIPFHGWAWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQF 300
                   PKK V G P+ GWAW L ++  +   +  +G A    ISP+G I Y+K++ +
Sbjct: 241 WLDEAKLPPKKAVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNY 300

Query: 301 IDDNQAGNENKDPKYLIAYTYSGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAAD 319
           I DN A   + DP  +  Y Y G TWI Y+   +I  K+  AK   +LGYF++++ AD
Sbjct: 301 IVDNGAATFH-DPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGAD 352

BLAST of Tan0012136 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 223.0 bits (567), Expect = 3.6e-58
Identity = 131/335 (39.10%), Postives = 189/335 (56.42%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQFSSTVRL 67
           V A YW A ++   P+A ID++LF+HLF  +  +N+ TY+V V        + F+ TVR 
Sbjct: 26  VKATYWFAESES--PLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVRR 85

Query: 68  RNPGIKTLLSIAGS---SDVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYPSSH 127
           RNP +KTLLSI G    +  FA MA  P +R  FI SSI++AR+  F GLDL W YPS  
Sbjct: 86  RNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSIT 145

Query: 128 DEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQYPVDAIKQNLDWVN 187
            EM+NF  LL  W  A+  +A +   P+LLL A V    Y  +++ +PV+A+  +LDWVN
Sbjct: 146 TEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADSLDWVN 205

Query: 188 VLSYDFYTPTSSSK-----------STGPS---------SAFHNPKKIVFGIPFHGWAWK 247
           +++YDFY   SS             +TGPS          A    KK V G P +G+AW 
Sbjct: 206 LVAYDFYESGSSRVTCSPAPLYDPITTGPSGDAGVRAWTQAGLPAKKAVLGFPLYGYAWC 265

Query: 248 LANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIAYTYSGT 307
           L ++  H+ ++ + G A    ISP+G I Y ++++FI DN+A     +   +  Y Y+  
Sbjct: 266 LTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKA-TMVYNSNLVQNYCYAKK 325

Query: 308 TWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADD 320
           TWI Y+   +I  K+  AK   +LGYF+++I ADD
Sbjct: 326 TWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADD 352

BLAST of Tan0012136 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 219.9 bits (559), Expect = 3.0e-57
Identity = 130/345 (37.68%), Postives = 191/345 (55.36%), Query Frame = 0

Query: 8   VNAGYWLASTDETLPVANIDATLFSHLFVGYVGVNSQTYEVTVDPSQAILINQFSSTVRL 67
           V A YW  +TD   P  +ID++LF+HLF  +  + +++YE+T+        + F+ TV+ 
Sbjct: 7   VKASYWFPATD--FPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTETVQQ 66

Query: 68  RNPGIKTLLSIAGSS---DVFAKMAEEPANRDAFIKSSIEVARAGEFDGLDLQWLYPSSH 127
           RNP +KTLLSI G +   D FA MA  P +R +FI+S+I VAR+  F GLDL W YP + 
Sbjct: 67  RNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYPRNE 126

Query: 128 DEMNNFESLLSFWHKAIVDDAINLSLPQLLLVATVSNVPYLQHNIQ---YPVDAIKQNLD 187
           +EM +F  LL  W  A+  ++ +     L+L A V    Y   N Q   YPV AI  +LD
Sbjct: 127 EEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLAISNSLD 186

Query: 188 WVNVLSYDFYTPTSSSKSTGPSSAFHNP--------------------KKIVFGIPFHGW 247
           W+N+++YDFY P   S  TGP ++ + P                    KK V G P++GW
Sbjct: 187 WINLMAYDFYGP-GWSTVTGPPASLYLPTDGRSGDSGVRDWTEAGLPAKKAVLGFPYYGW 246

Query: 248 AWKLANSHQHDIFSKAEGVATGEYISPNGQIYYSKVKQFIDDNQAGNENKDPKYLIAYTY 307
           AW LA+   +      +   TG  IS +G+I Y +++ +I DN A  +  D   +  Y Y
Sbjct: 247 AWTLADPDVNGY----DANTTGPAISDDGEISYRQLQTWIVDNGA-TKVHDDMMVGDYCY 306

Query: 308 SGTTWIAYESEYTIAAKISLAKSDNVLGYFAFNIAADDVKAWSSS 327
           +GTTWI Y+SE +I  K+  AK   +LGYF++++  DD    SS+
Sbjct: 307 AGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDNSELSSA 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818628.0e-6339.71Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A072UR654.7e-4733.62Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
U5N4E38.9e-4633.80Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
A0A1B1J8Z21.5e-3730.66Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
A0A072VEP01.9e-3529.60Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022999580.17.5e-13570.09chitotriosidase-1-like [Cucurbita maxima][more]
XP_022946077.18.3e-13470.09acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mamm... [more]
XP_023521369.18.3e-13469.50acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo][more]
KAG6599362.11.4e-13369.50Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG6599364.1... [more]
XP_022999577.12.4e-13369.50chitotriosidase-1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1KFT33.6e-13570.09chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1[more]
A0A6J1G2P44.0e-13470.09acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE... [more]
A0A6J1KB821.2e-13369.50chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1[more]
A0A6J1G4H51.1e-12868.04chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 ... [more]
A0A6J1G4J99.6e-12867.85acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G19810.15.7e-6439.71Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19750.19.4e-5937.82Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19760.11.2e-5837.71Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.13.6e-5839.10Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.13.0e-5737.68Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011583Chitinase IISMARTSM006362g34coord: 7..318
e-value: 6.1E-55
score: 198.5
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..207
e-value: 1.9E-46
score: 160.9
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 208..319
e-value: 4.4E-22
score: 81.0
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 5..210
coord: 210..328
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 5..210
coord: 210..328
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 10..318
e-value: 2.2E-50
score: 172.2
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 7..335
score: 44.340134
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 218..289
e-value: 4.4E-22
score: 81.0
IPR029070Chitinase insertion domain superfamilySUPERFAMILY54556Chitinase insertion domaincoord: 220..289
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 10..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0012136.1Tan0012136.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0008061 chitin binding
molecular_function GO:0016787 hydrolase activity