Tan0011787 (gene) Snake gourd v1

Overview
NameTan0011787
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionsubtilisin-like protease SBT1.8
LocationLG09: 62866601 .. 62869050 (-)
RNA-Seq ExpressionTan0011787
SyntenyTan0011787
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGACCATGGCTAGATTTCTAATCGTTTCTCTTCTTCTTCTTCTTCTTCCTTGTGTCTTCGTCAATGGCAAGAGAACCTACATTGTTCGCATGAAACATCATGCTCTGCCGTCTGATTATCCCACCCACCATGACTGGTACTCTGCCCATCTGCAATCTCTATCCTCCTCTGATTCCCTTCTCTACACCTACACCTCCGCCTACCATGGCTTCGCTGCTTCTCTGGATCCAGAGGAAGTCGAGTTGCTCAGCCAATCGGATTCCGTTCTGGGTGTCTACGAGGACACTGTTTACAACCTTCACACCACTCGAACTCCGGGATTCCTCGGCCTCGACTCCAATTTTGGCTTGTGGGAAGGTCACAACACTCAGGATCTCGACCAGGCTTCTCACGATGTCATCATTGGTGTTCTCGACACCGGGATTTGGCCGGAGTCTAAGAGCTTTGATGACACTGGAATGCCGGAGATCCCATCACGGTGGCGTGGAGCATGCGAATCGGGTCCCGATTTCAGCCCTTCACTCTGCAACAAGAAACTAATCGGAGCTCGCAGCTTCTCGAAGGGTTATCAGATGGCTTCCGGTGGTGGGTACTTCAGAAAACCCAGAGAAAACCAGTCGCCTCGAGACCAGGACGGGCACGGCACTCACACAGCTAGCACGGCTGCTGGGTCACATGTGGCTAACGCGAGCTTACTTGGGTATGCCAGAGGCATTGCTAGGGGGATGGCTCCTCAAGCGAGAGTTGCCGCTTACAAAACTTGTTGGCCCACTGGTTGCTTTGGATCTGACATTCTTGCAGGTATGGATCAGGCAATATCCGACGGCGTCGATGTACTCTCACTCTCTTTGGGTGGTGGCTCTGCACCGTATTACCGGGATACCATTGCAATCGGAGCATTTGCAGCCATGGAGAAGGGGGTTTTCGTTTCGTGCTCGGCTGGGAACAGTGGACCAAACAAGGCCTCTCTGGCCAATGTGGCGCCGTGGATCATGACAGTCGGAGCCGGAACTCTGGACCGGGACTTCCCAGCTTATGTCCAACTCGGAAATGGAAAGCGGTTCACTGGAGTGTCGCTCTACAGTGGCCAAGGAATGGGGAACAAGCCGGTGGCTTTGGTGTACAACAAAGGAAGCAACACCTCGAGCAACATGTGCTTGCCCGGATCTCTTGAGCCGGCGTTGGTTAGAGGAAAAGTTGTGATATGCGACAGAGGAATCAACGCCAGAGTAGAGAAGGGAGGGGTGGTGCGAGATGCAGGTGGAATTGGAATGATTCTGGCAAACACGGCCGCTAGTGGGGAGGAATTGGTGGCTGATAGCCACTTGCTACCGGCCGTCGCGGTGGGGAGAAAAACCGGCGACTTGATCCGGCAGTATGTGCGGTCAGTTTCGAAGCCGACGGCGGTGCTGAGCTTTGGTGGGACAGTTTTGAACGTGCGCCCATCTCCAGTTGTGGCGGCCTTTAGTTCCAGAGGACCCAACTTGGTAACGCCCCAAATCCTAAAGCCGGACGTTATTGGGCCTGGCGTTAACATCTTGGCTGCCTGGTCTGAATCCATTGGGCCAACTGGATTGGACAGTGACAAGCGAAAAACTCAGTTCAATATTATGTCAGGTATATCCTAAATGCCTCTTCTTACTGTACTGATCCACTGTCCTGGCGATTTCAAATTAATGGCTCAGTTCTGTCTTCAACTCTGTTACTAACCCATTTGAAAGCCAGTTGTTTTCTAGGCAAATAATGTCCATTCTGTAACGGCTAAATCTTCTGCTATGTAGGAACATCCATGTCTTGCCCACATATAAGTGGGCTGGCGGCATTACTGAAGGCAGCTCATCCAGAATGGAGTCCAAGTGCTATCAAATCTGCGTTAATGACCACTGCATACACGCAGGACAACACCAACTCATCTCTCAGGGATGCTGCCGGAGGGGCGTTTTCCAATCCATGGGCTCATGGAGCTGGCCATGTGGATCCTCACAAAGCCCTCTCCCCCGGTCTTGTATACGATATCTCGATCGATGACTACATTGCCTTCTTGTGCACCTTGGACTACGGGACCGATCATGTTCAGGCAATTGTAAAGCGTTCGAACATAACCTGCTCCAGAAAGTTTGCTGACCCAGGACAACTTAACTACCCTTCATTCTCAATTGTGTTTGGGAGCAAGAGGGTTGTTCGGTACACTCGTGAAGTCACGAATGTTGGGGCCGCAGGGTCGGTTTATGAAGTGGCTACTACTGCACCATCAGTTGTGAAGGTGATGGTGAAACCCTCAAAACTTGTGTTCACTAAAGTTGGGGAGAAAAAGAGGTACACTGTTACATTTGTGGCAAGCAGGGACGCTGCTCAAACCACGAGATTTGGGTTTGGATCGATTGTATGGAGAAATGACCTACACCAAGTCAGAAGCCCAGTGGCATTCGCCTGGACAAGGTTGTGA

mRNA sequence

ATGGGGACCATGGCTAGATTTCTAATCGTTTCTCTTCTTCTTCTTCTTCTTCCTTGTGTCTTCGTCAATGGCAAGAGAACCTACATTGTTCGCATGAAACATCATGCTCTGCCGTCTGATTATCCCACCCACCATGACTGGTACTCTGCCCATCTGCAATCTCTATCCTCCTCTGATTCCCTTCTCTACACCTACACCTCCGCCTACCATGGCTTCGCTGCTTCTCTGGATCCAGAGGAAGTCGAGTTGCTCAGCCAATCGGATTCCGTTCTGGGTGTCTACGAGGACACTGTTTACAACCTTCACACCACTCGAACTCCGGGATTCCTCGGCCTCGACTCCAATTTTGGCTTGTGGGAAGGTCACAACACTCAGGATCTCGACCAGGCTTCTCACGATGTCATCATTGGTGTTCTCGACACCGGGATTTGGCCGGAGTCTAAGAGCTTTGATGACACTGGAATGCCGGAGATCCCATCACGGTGGCGTGGAGCATGCGAATCGGGTCCCGATTTCAGCCCTTCACTCTGCAACAAGAAACTAATCGGAGCTCGCAGCTTCTCGAAGGGTTATCAGATGGCTTCCGGTGGTGGGTACTTCAGAAAACCCAGAGAAAACCAGTCGCCTCGAGACCAGGACGGGCACGGCACTCACACAGCTAGCACGGCTGCTGGGTCACATGTGGCTAACGCGAGCTTACTTGGGTATGCCAGAGGCATTGCTAGGGGGATGGCTCCTCAAGCGAGAGTTGCCGCTTACAAAACTTGTTGGCCCACTGGTTGCTTTGGATCTGACATTCTTGCAGGTATGGATCAGGCAATATCCGACGGCGTCGATGTACTCTCACTCTCTTTGGGTGGTGGCTCTGCACCGTATTACCGGGATACCATTGCAATCGGAGCATTTGCAGCCATGGAGAAGGGGGTTTTCGTTTCGTGCTCGGCTGGGAACAGTGGACCAAACAAGGCCTCTCTGGCCAATGTGGCGCCGTGGATCATGACAGTCGGAGCCGGAACTCTGGACCGGGACTTCCCAGCTTATGTCCAACTCGGAAATGGAAAGCGGTTCACTGGAGTGTCGCTCTACAGTGGCCAAGGAATGGGGAACAAGCCGGTGGCTTTGGTGTACAACAAAGGAAGCAACACCTCGAGCAACATGTGCTTGCCCGGATCTCTTGAGCCGGCGTTGGTTAGAGGAAAAGTTGTGATATGCGACAGAGGAATCAACGCCAGAGTAGAGAAGGGAGGGGTGGTGCGAGATGCAGGTGGAATTGGAATGATTCTGGCAAACACGGCCGCTAGTGGGGAGGAATTGGTGGCTGATAGCCACTTGCTACCGGCCGTCGCGGTGGGGAGAAAAACCGGCGACTTGATCCGGCAGTATGTGCGGTCAGTTTCGAAGCCGACGGCGGTGCTGAGCTTTGGTGGGACAGTTTTGAACGTGCGCCCATCTCCAGTTGTGGCGGCCTTTAGTTCCAGAGGACCCAACTTGGTAACGCCCCAAATCCTAAAGCCGGACGTTATTGGGCCTGGCGTTAACATCTTGGCTGCCTGGTCTGAATCCATTGGGCCAACTGGATTGGACAGTGACAAGCGAAAAACTCAGTTCAATATTATGTCAGGAACATCCATGTCTTGCCCACATATAAGTGGGCTGGCGGCATTACTGAAGGCAGCTCATCCAGAATGGAGTCCAAGTGCTATCAAATCTGCGTTAATGACCACTGCATACACGCAGGACAACACCAACTCATCTCTCAGGGATGCTGCCGGAGGGGCGTTTTCCAATCCATGGGCTCATGGAGCTGGCCATGTGGATCCTCACAAAGCCCTCTCCCCCGGTCTTGTATACGATATCTCGATCGATGACTACATTGCCTTCTTGTGCACCTTGGACTACGGGACCGATCATGTTCAGGCAATTGTAAAGCGTTCGAACATAACCTGCTCCAGAAAGTTTGCTGACCCAGGACAACTTAACTACCCTTCATTCTCAATTGTGTTTGGGAGCAAGAGGGTTGTTCGGTACACTCGTGAAGTCACGAATGTTGGGGCCGCAGGGTCGGTTTATGAAGTGGCTACTACTGCACCATCAGTTGTGAAGGTGATGGTGAAACCCTCAAAACTTGTGTTCACTAAAGTTGGGGAGAAAAAGAGGTACACTGTTACATTTGTGGCAAGCAGGGACGCTGCTCAAACCACGAGATTTGGGTTTGGATCGATTGTATGGAGAAATGACCTACACCAAGTCAGAAGCCCAGTGGCATTCGCCTGGACAAGGTTGTGA

Coding sequence (CDS)

ATGGGGACCATGGCTAGATTTCTAATCGTTTCTCTTCTTCTTCTTCTTCTTCCTTGTGTCTTCGTCAATGGCAAGAGAACCTACATTGTTCGCATGAAACATCATGCTCTGCCGTCTGATTATCCCACCCACCATGACTGGTACTCTGCCCATCTGCAATCTCTATCCTCCTCTGATTCCCTTCTCTACACCTACACCTCCGCCTACCATGGCTTCGCTGCTTCTCTGGATCCAGAGGAAGTCGAGTTGCTCAGCCAATCGGATTCCGTTCTGGGTGTCTACGAGGACACTGTTTACAACCTTCACACCACTCGAACTCCGGGATTCCTCGGCCTCGACTCCAATTTTGGCTTGTGGGAAGGTCACAACACTCAGGATCTCGACCAGGCTTCTCACGATGTCATCATTGGTGTTCTCGACACCGGGATTTGGCCGGAGTCTAAGAGCTTTGATGACACTGGAATGCCGGAGATCCCATCACGGTGGCGTGGAGCATGCGAATCGGGTCCCGATTTCAGCCCTTCACTCTGCAACAAGAAACTAATCGGAGCTCGCAGCTTCTCGAAGGGTTATCAGATGGCTTCCGGTGGTGGGTACTTCAGAAAACCCAGAGAAAACCAGTCGCCTCGAGACCAGGACGGGCACGGCACTCACACAGCTAGCACGGCTGCTGGGTCACATGTGGCTAACGCGAGCTTACTTGGGTATGCCAGAGGCATTGCTAGGGGGATGGCTCCTCAAGCGAGAGTTGCCGCTTACAAAACTTGTTGGCCCACTGGTTGCTTTGGATCTGACATTCTTGCAGGTATGGATCAGGCAATATCCGACGGCGTCGATGTACTCTCACTCTCTTTGGGTGGTGGCTCTGCACCGTATTACCGGGATACCATTGCAATCGGAGCATTTGCAGCCATGGAGAAGGGGGTTTTCGTTTCGTGCTCGGCTGGGAACAGTGGACCAAACAAGGCCTCTCTGGCCAATGTGGCGCCGTGGATCATGACAGTCGGAGCCGGAACTCTGGACCGGGACTTCCCAGCTTATGTCCAACTCGGAAATGGAAAGCGGTTCACTGGAGTGTCGCTCTACAGTGGCCAAGGAATGGGGAACAAGCCGGTGGCTTTGGTGTACAACAAAGGAAGCAACACCTCGAGCAACATGTGCTTGCCCGGATCTCTTGAGCCGGCGTTGGTTAGAGGAAAAGTTGTGATATGCGACAGAGGAATCAACGCCAGAGTAGAGAAGGGAGGGGTGGTGCGAGATGCAGGTGGAATTGGAATGATTCTGGCAAACACGGCCGCTAGTGGGGAGGAATTGGTGGCTGATAGCCACTTGCTACCGGCCGTCGCGGTGGGGAGAAAAACCGGCGACTTGATCCGGCAGTATGTGCGGTCAGTTTCGAAGCCGACGGCGGTGCTGAGCTTTGGTGGGACAGTTTTGAACGTGCGCCCATCTCCAGTTGTGGCGGCCTTTAGTTCCAGAGGACCCAACTTGGTAACGCCCCAAATCCTAAAGCCGGACGTTATTGGGCCTGGCGTTAACATCTTGGCTGCCTGGTCTGAATCCATTGGGCCAACTGGATTGGACAGTGACAAGCGAAAAACTCAGTTCAATATTATGTCAGGAACATCCATGTCTTGCCCACATATAAGTGGGCTGGCGGCATTACTGAAGGCAGCTCATCCAGAATGGAGTCCAAGTGCTATCAAATCTGCGTTAATGACCACTGCATACACGCAGGACAACACCAACTCATCTCTCAGGGATGCTGCCGGAGGGGCGTTTTCCAATCCATGGGCTCATGGAGCTGGCCATGTGGATCCTCACAAAGCCCTCTCCCCCGGTCTTGTATACGATATCTCGATCGATGACTACATTGCCTTCTTGTGCACCTTGGACTACGGGACCGATCATGTTCAGGCAATTGTAAAGCGTTCGAACATAACCTGCTCCAGAAAGTTTGCTGACCCAGGACAACTTAACTACCCTTCATTCTCAATTGTGTTTGGGAGCAAGAGGGTTGTTCGGTACACTCGTGAAGTCACGAATGTTGGGGCCGCAGGGTCGGTTTATGAAGTGGCTACTACTGCACCATCAGTTGTGAAGGTGATGGTGAAACCCTCAAAACTTGTGTTCACTAAAGTTGGGGAGAAAAAGAGGTACACTGTTACATTTGTGGCAAGCAGGGACGCTGCTCAAACCACGAGATTTGGGTTTGGATCGATTGTATGGAGAAATGACCTACACCAAGTCAGAAGCCCAGTGGCATTCGCCTGGACAAGGTTGTGA

Protein sequence

MGTMARFLIVSLLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSSSDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSNFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFADPGQLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGEKKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL
Homology
BLAST of Tan0011787 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 543/754 (72.02%), Postives = 630/754 (83.55%), Query Frame = 0

Query: 8   LIVSLLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSSSDSLLYTYTS 67
           +I + L LLL       K+TYI+R+ H   P  + THHDWY++    L+S  SLLYTYT+
Sbjct: 13  IITTFLFLLL---HTTAKKTYIIRVNHSDKPESFLTHHDWYTS---QLNSESSLLYTYTT 72

Query: 68  AYHGFAASLDPEEVE-LLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSNFGLWEGHNTQD 127
           ++HGF+A LD  E + LLS S+S+L ++ED +Y LHTTRTP FLGL+S FG+       D
Sbjct: 73  SFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------HD 132

Query: 128 LDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPSLCNKKLIGARS 187
           L  +S+ VIIGVLDTG+WPES+SFDDT MPEIPS+W+G CESG DF   LCNKKLIGARS
Sbjct: 133 LGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARS 192

Query: 188 FSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAP 247
           FSKG+QMASGGG+  K RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G ARGMA 
Sbjct: 193 FSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMAT 252

Query: 248 QARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAME 307
           +ARVA YK CW TGCFGSDILA MD+AI DGVDVLSLSLGGGSAPYYRDTIAIGAF+AME
Sbjct: 253 RARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAME 312

Query: 308 KGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQG 367
           +GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG G
Sbjct: 313 RGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVG 372

Query: 368 MGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGGVVRDAGGIGM 427
           MG KP+ LVYNKG+++SSN+CLPGSL+ ++VRGK+V+CDRG+NARVEKG VVRDAGG+GM
Sbjct: 373 MGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGM 432

Query: 428 ILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTVLNVRPSPV 487
           I+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YV+S SKPTA+L F GTVL+V+PSPV
Sbjct: 433 IMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPV 492

Query: 488 VAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSC 547
           VAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSGTSMSC
Sbjct: 493 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 552

Query: 548 PHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVD 607
           PHISGLA LLKAAHPEWSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GHVD
Sbjct: 553 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 612

Query: 608 PHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFADPGQLNYPSFSI 667
           P KALSPGLVYDIS ++YI FLC+LDY  DH+ AIVKR ++ CS+KF+DPGQLNYPSFS+
Sbjct: 613 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 672

Query: 668 VFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGEKKRYTVTFVA 727
           +FG KRVVRYTREVTNVGAA SVY+V       V + VKPSKL F  VGEKKRYTVTFV+
Sbjct: 673 LFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 732

Query: 728 SRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTR 761
            +  + T +  FGSI W N  H+VRSPVAF+W R
Sbjct: 733 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of Tan0011787 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 1.5e-234
Identity = 421/739 (56.97%), Postives = 527/739 (71.31%), Query Frame = 0

Query: 27  TYIVRMKHHALPSDYPTHHDWYSAHLQSLSSSDSLLYTYTSAYHGFAASLDPEEVELLSQ 86
           TYIV M    +PS +  H +WY + L+S+S S  LLYTY +A HGF+  L  EE + L  
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFSTRLTQEEADSLMT 90

Query: 87  SDSVLGVYEDTVYNLHTTRTPGFLGLDSNFGLWEGHNTQDL--DQASH-DVIIGVLDTGI 146
              V+ V  +  Y LHTTRTP FLGLD         +T DL  +  S+ DV++GVLDTG+
Sbjct: 91  QPGVISVLPEHRYELHTTRTPLFLGLD--------EHTADLFPEAGSYSDVVVGVLDTGV 150

Query: 147 WPESKSFDDTGMPEIPSRWRGACESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKP 206
           WPESKS+ D G   IPS W+G CE+G +F+ SLCN+KLIGAR F++GY+  S  G   + 
Sbjct: 151 WPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE--STMGPIDES 210

Query: 207 RENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFG 266
           +E++SPRD DGHGTHT+STAAGS V  ASLLGYA G ARGMAP+ARVA YK CW  GCF 
Sbjct: 211 KESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFS 270

Query: 267 SDILAGMDQAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKA 326
           SDILA +D+AI+D V+VLS+SLGGG + YYRD +AIGAFAAME+G+ VSCSAGN+GP+ +
Sbjct: 271 SDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSS 330

Query: 327 SLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVALVY--NKGSN 386
           SL+NVAPWI TVGAGTLDRDFPA   LGNGK FTGVSL+ G+ + +K +  +Y  N  + 
Sbjct: 331 SLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNA 390

Query: 387 TSSNMCLPGSLEPALVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 446
           T+ N+C+ G+L P  V+GK+V+CDRGINARV+KG VV+ AGG+GMILANTAA+GEELVAD
Sbjct: 391 TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVAD 450

Query: 447 SHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 506
           +HLLPA  VG K GD+IR YV +   PTA +S  GTV+ V+PSPVVAAFSSRGPN +TP 
Sbjct: 451 AHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPN 510

Query: 507 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 566
           ILKPD+I PGVNILAAW+ + GPTGL SD R+ +FNI+SGTSMSCPH+SGLAALLK+ HP
Sbjct: 511 ILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHP 570

Query: 567 EWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLVYDISI 626
           EWSP+AI+SALMTTAY        L D A G  S P+ HGAGHV P  A +PGL+YD++ 
Sbjct: 571 EWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTT 630

Query: 627 DDYIAFLCTLDYGTDHVQAIVKRSNITCS-RKFADPGQLNYPSFSIVFGSKRVVRYTREV 686
           +DY+ FLC L+Y +  +++ V R N TC   K      LNYPSF++        +YTR V
Sbjct: 631 EDYLGFLCALNYTSPQIRS-VSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTV 690

Query: 687 TNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGEKKRYTVTFVASRDAAQTTRFGFGS 746
           T+VG AG+     T+  + VK+ V+P+ L F +  EKK YTVTF         +   FGS
Sbjct: 691 TSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-SFGS 750

Query: 747 IVWRNDLHQVRSPVAFAWT 760
           I W +  H V SPVA +WT
Sbjct: 751 IEWSDGKHVVGSPVAISWT 757

BLAST of Tan0011787 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 8.9e-219
Identity = 415/777 (53.41%), Postives = 521/777 (67.05%), Query Frame = 0

Query: 4   MARFLIVSLLLL--LLPCVF------VNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSL 63
           MA+  + S+  +  LL C F       +G  +YIV ++    PS + +H++W+ + L+SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 64  SSSD---SLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGL 123
            SS    +LLY+Y+ A HGF+A L P +   L +  SV+ V  D    +HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 124 DSNFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDF 183
             N GLW   N         DVI+GVLDTGIWPE  SF D+G+  IPS W+G CE GPDF
Sbjct: 121 SQNSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 184 SPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANAS 243
             S CN+KLIGAR+F +GY     G      +E++SPRD +GHGTHTASTAAGS VANAS
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 244 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLG-GGSAP 303
           L  YARG A GMA +AR+AAYK CW  GC+ SDILA MDQA++DGV V+SLS+G  GSAP
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 304 -YYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQL 363
            Y+ D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 364 GNGKRFTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINA 423
           G+GK FTG SLY+G+ + +  ++LVY+   +  S +C PG L  +LV GK+V+CDRG NA
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNA 420

Query: 424 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTA 483
           RVEKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++   PTA
Sbjct: 421 RVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTA 480

Query: 484 VLSFGGTVLNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDS 543
            +SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD 
Sbjct: 481 KISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDI 540

Query: 544 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDA 603
           D R+ QFNI+SGTSMSCPH+SGLAALL+ AHP+WSP+AIKSAL+TTAY  +N+   + D 
Sbjct: 541 DPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL 600

Query: 604 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNI-- 663
           A G  SN + HGAGHVDP+KAL+PGLVYDI + +Y+AFLC + Y    +   ++   +  
Sbjct: 601 ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYD 660

Query: 664 TC-SRKFADPGQLNYPSFSIVFGSK-RVVRYTREVTNVGA-AGSVYEVATTAPSVVKVMV 723
            C + K    G LNYPSFS+VF S   VV+Y R V NVG+   +VYEV   +P+ V++ V
Sbjct: 661 ACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDV 720

Query: 724 KPSKLVFTKVGEKKRYTVTF---VASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAW 759
            PSKL F+K      Y VTF   V            FGSI W +  H V+SPVA  W
Sbjct: 721 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Tan0011787 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.5e-218
Identity = 401/773 (51.88%), Postives = 519/773 (67.14%), Query Frame = 0

Query: 7   FLIVSLLLLLLPC---VFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLS------- 66
           F+I+S+ L+ L       ++ K+TY++ M   A+P  Y  H  WYS+ + S++       
Sbjct: 13  FIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEE 72

Query: 67  --SSDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 126
             +++ +LYTY +A+HG AA L  EE E L + D V+ V  +T Y LHTTR+P FLGL+ 
Sbjct: 73  EGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLER 132

Query: 127 NFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSP 186
                E           HDV++GVLDTGIWPES+SF+DTGM  +P+ WRGACE+G  F  
Sbjct: 133 Q----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLK 192

Query: 187 SLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLL 246
             CN+K++GAR F +GY+ A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+L 
Sbjct: 193 RNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLF 252

Query: 247 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYR 306
           G+A G ARGMA +ARVAAYK CW  GCF SDIL+ +DQA++DGV VLS+SLGGG + Y R
Sbjct: 253 GFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSR 312

Query: 307 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGK 366
           D+++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G  +
Sbjct: 313 DSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMR 372

Query: 367 RFTGVSLYSGQGM--GNKPVALVY---NKGSNTSSNMCLPGSLEPALVRGKVVICDRGIN 426
            F GVSLY G+ +   NK   LVY   N  S   ++ CL G+L+   V GK+VICDRG+ 
Sbjct: 373 TFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVT 432

Query: 427 ARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPT 486
            RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K T
Sbjct: 433 PRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKAT 492

Query: 487 AVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDS 546
           A L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L S
Sbjct: 493 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 552

Query: 547 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDA 606
           D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP+WSP+AIKSALMTTAY  DN    L DA
Sbjct: 553 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDA 612

Query: 607 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITC 666
           +G A S+P+ HGAGH+DP +A  PGLVYDI   +Y  FLCT D     ++   K SN TC
Sbjct: 613 SGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC 672

Query: 667 SRKFA-DPGQLNYPSFSIVFGSK---RVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVK 726
               A +PG LNYP+ S +F      + +   R VTNVG   S Y+V+ +      V V+
Sbjct: 673 KHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQ 732

Query: 727 PSKLVFTKVGEKKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAW 759
           P  L FT   +K  YTVTF   R   +  R  FG +VW++  H+VRSPV   W
Sbjct: 733 PKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Tan0011787 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 5.8e-210
Identity = 389/749 (51.94%), Postives = 508/749 (67.82%), Query Frame = 0

Query: 27  TYIVRMKHHALPSDYPTHHDWYSAHLQSLSSS-DSLLYTYTSAYHGFAASLDPEEVELLS 86
           TYIV + H A PS +PTH  WY++ L SL+SS  S+++TY + +HGF+A L  ++   L 
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLL 86

Query: 87  QSDSVLGVYEDTVYNLHTTRTPGFLGLDS--NFGLWEGHNTQDLDQASHDVIIGVLDTGI 146
               V+ V  + V +LHTTR+P FLGL S    GL E     + D  S D++IGV+DTG+
Sbjct: 87  DHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE-----ESDFGS-DLVIGVIDTGV 146

Query: 147 WPESKSFDDTGMPEIPSRWRGACESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKP 206
           WPE  SFDD G+  +P +W+G C +  DF  S CN+KL+GAR F  GY+  +  G   + 
Sbjct: 147 WPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN--GKMNET 206

Query: 207 RENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFG 266
            E +SPRD DGHGTHTAS +AG +V  AS LGYA G+A GMAP+AR+AAYK CW +GC+ 
Sbjct: 207 TEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYD 266

Query: 267 SDILAGMDQAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKA 326
           SDILA  D A++DGVDV+SLS+GG   PYY D IAIGAF A+++G+FVS SAGN GP   
Sbjct: 267 SDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGAL 326

Query: 327 SLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMG-NKPVALVYN----K 386
           ++ NVAPW+ TVGAGT+DRDFPA V+LGNGK  +GVS+Y G G+   +   LVY      
Sbjct: 327 TVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLG 386

Query: 387 GSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEEL 446
           G   SS++CL GSL+P LV+GK+V+CDRGIN+R  KG +VR  GG+GMI+AN    GE L
Sbjct: 387 GDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGL 446

Query: 447 VADSHLLPAVAVGRKTGDLIRQYV------RSVSKPTAVLSFGGTVLNVRPSPVVAAFSS 506
           VAD H+LPA +VG   GD IR+Y+      RS   PTA + F GT L +RP+PVVA+FS+
Sbjct: 447 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 506

Query: 507 RGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGL 566
           RGPN  TP+ILKPDVI PG+NILAAW + IGP+G+ SD R+T+FNI+SGTSM+CPH+SGL
Sbjct: 507 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 566

Query: 567 AALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALS 626
           AALLKAAHP+WSP+AI+SAL+TTAYT DN+   + D + G  S+   +G+GHV P KA+ 
Sbjct: 567 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 626

Query: 627 PGLVYDISIDDYIAFLCTLDYGTDHVQAIVKR-SNITCSRKFADPGQLNYPSFSIVF--- 686
           PGLVYDI+  DYI FLC  +Y   ++  I +R ++   +R+    G LNYPSFS+VF   
Sbjct: 627 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQY 686

Query: 687 -GSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGEKKRYTVTFVAS 746
             SK    + R VTNVG + SVYE+    P    V V+P KL F +VG+K  + V    +
Sbjct: 687 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 746

Query: 747 --RDAAQTTRFGFGSIVWRNDLHQVRSPV 755
             + +   T    G IVW +    V SP+
Sbjct: 747 EVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of Tan0011787 vs. NCBI nr
Match: KAG6590040.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1441.4 bits (3730), Expect = 0.0e+00
Identity = 722/765 (94.38%), Postives = 740/765 (96.73%), Query Frame = 0

Query: 1   MGTMARFLIVS-LLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSS-- 60
           MG+MAR L+ S LLLLLLPCVFVN KRTYIVRMKHHALPSDY THHDWYSAHLQSLSS  
Sbjct: 1   MGSMARLLVASFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSA 60

Query: 61  -SDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSNF 120
            SDSLLYTYTSAYHGFAASLDP+E ELL QSDSVLGVYEDTVYNLHTTRTPGFLGLDS+F
Sbjct: 61  TSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPSL 180
           GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CESGPDFSPSL
Sbjct: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGG 420
           TGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQF
Sbjct: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLVYDIS +DYI FLC+LDYG DHVQAI KRSNITC +KFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFADP 660

Query: 661 GQLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGE 720
           GQLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVYEVATTAPSVVKV VKPSKLVF+KVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGE 720

Query: 721 KKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL 762
           +KRYTVTFVASRDAA+TTR+GFGSI W ND HQVRSPVAFAWT+L
Sbjct: 721 RKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Tan0011787 vs. NCBI nr
Match: XP_022960792.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-like protease SBT1.8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1441.0 bits (3729), Expect = 0.0e+00
Identity = 722/766 (94.26%), Postives = 740/766 (96.61%), Query Frame = 0

Query: 1   MGTMARFLIVS--LLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSS- 60
           MG+MAR L+ S  LLLLLLPCVFVN KRTYIVRMKHHALPSDY THHDWYSAHLQSLSS 
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  --SDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSN 120
             SDSLLYTYTSAYHGFAASLDP+E ELL QSDSVLGVYEDTVYNLHTTRTPGFLGLDS+
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGLVYDIS +DYI FLC+LDYG DHVQAI KRSNITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVG 720
           PGQLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVYEVATTAPSVVKV VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 EKKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL 762
           E+KRYTVTFVASRDAA+TTR+GFGSI W ND HQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Tan0011787 vs. NCBI nr
Match: XP_022987083.1 (subtilisin-like protease SBT1.8 [Cucurbita maxima])

HSP 1 Score: 1439.5 bits (3725), Expect = 0.0e+00
Identity = 721/766 (94.13%), Postives = 740/766 (96.61%), Query Frame = 0

Query: 1   MGTMARFLIVS--LLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSS- 60
           MG+MAR L+ S  LLLLLLPCVFVN KRTYIVRMKHHALPSDY THHDWYSAHLQSLSS 
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  --SDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSN 120
             SDSLLYTYTSAYHGFAASLDP+E ELL QSDSVLGVYEDTVY LHTTRTPGFLGLDS+
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDIS +DYI FLC+LDYG DHVQAIVKRSNITCS+KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVG 720
           PGQLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVYEVATTAPSVVKV VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 EKKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL 762
           E+KRYTVTFVASRDAA+TTR+GFGSI W ND HQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Tan0011787 vs. NCBI nr
Match: XP_023516159.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1437.2 bits (3719), Expect = 0.0e+00
Identity = 720/765 (94.12%), Postives = 738/765 (96.47%), Query Frame = 0

Query: 1   MGTMARFLIV-SLLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSS-- 60
           MG+MA  L+   LLLLLLPCVFVN KRTYIVRMKHHALPSDY THHDWYSAHLQSLSS  
Sbjct: 1   MGSMASLLVAYFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSA 60

Query: 61  -SDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSNF 120
            SDSLLYTYTSAYHGFAASLDP+E ELL QSDSVLGVYEDTVYNLHTTRTPGFLGLDS+F
Sbjct: 61  TSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPSL 180
           GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CESGPDFSPSL
Sbjct: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGG 420
           TGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQF
Sbjct: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLVYDIS +DYI FLC+LDYG DHVQAI KRSNITCS+KFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCSKKFADP 660

Query: 661 GQLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGE 720
           GQLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVYEVATTAPSVVKV VKPSKLVF+KVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGE 720

Query: 721 KKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL 762
           +KRYTVTFVASRDAA+TTR+GFGSI W ND HQVRSPVAFAWT+L
Sbjct: 721 RKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Tan0011787 vs. NCBI nr
Match: XP_038879224.1 (subtilisin-like protease SBT1.8 [Benincasa hispida])

HSP 1 Score: 1421.4 bits (3678), Expect = 0.0e+00
Identity = 712/764 (93.19%), Postives = 736/764 (96.34%), Query Frame = 0

Query: 1   MGTMARFLIVSLLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSS--- 60
           MG+MAR L+++ LLLLLPCVFVN K+TYIVRMKH+ALPS+Y THHDWYSAHLQSLSS   
Sbjct: 1   MGSMAR-LLIAFLLLLLPCVFVNAKKTYIVRMKHNALPSEYLTHHDWYSAHLQSLSSSST 60

Query: 61  SDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSNFG 120
           SDSLLYTYTSAYHGFAASLD  E ELL QSDSVLGVYED+VY LHTTRTPGFLGLDS+FG
Sbjct: 61  SDSLLYTYTSAYHGFAASLDSNEAELLRQSDSVLGVYEDSVYTLHTTRTPGFLGLDSDFG 120

Query: 121 LWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPSLC 180
           LWEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CESG DFSPSLC
Sbjct: 121 LWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGADFSPSLC 180

Query: 181 NKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYA 240
           NKKLIGARSFSKGYQMASGGGYFRKPREN+SPRDQDGHGTHTASTAAGSHVANASLLGYA
Sbjct: 181 NKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYA 240

Query: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRDTI 300
           RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRDTI
Sbjct: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTI 300

Query: 301 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT 360
           AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQ+GNGKRFT
Sbjct: 301 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQIGNGKRFT 360

Query: 361 GVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGGV 420
           GVSLYSGQGMG+K V LVYNKGSNTSSNMCLPGSLEPA+VRGKVV+CDRGINARVEKGGV
Sbjct: 361 GVSLYSGQGMGSKAVELVYNKGSNTSSNMCLPGSLEPAVVRGKVVVCDRGINARVEKGGV 420

Query: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGT 480
           VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS S PTAVLSFGGT
Sbjct: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGT 480

Query: 481 VLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFN 540
           +LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFN
Sbjct: 481 ILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFN 540

Query: 541 IMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNP 600
           IMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNP
Sbjct: 541 IMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNP 600

Query: 601 WAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFADPG 660
           WAHGAGHVDPHKALSPGL+YDIS +DYIAFLC+LDYG DHVQAIVKRSNITCSRKFADPG
Sbjct: 601 WAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPG 660

Query: 661 QLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGEK 720
           QLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVYEVATTAPSVVKV VKPSKLVFTKVGE+
Sbjct: 661 QLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFTKVGER 720

Query: 721 KRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL 762
           KRYTVTFVASRDAAQT RFGFGSI W N  HQVRSPVAFAWTRL
Sbjct: 721 KRYTVTFVASRDAAQTMRFGFGSIAWNNYQHQVRSPVAFAWTRL 763

BLAST of Tan0011787 vs. ExPASy TrEMBL
Match: A0A6J1H8F1 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE=3 SV=1)

HSP 1 Score: 1441.0 bits (3729), Expect = 0.0e+00
Identity = 722/766 (94.26%), Postives = 740/766 (96.61%), Query Frame = 0

Query: 1   MGTMARFLIVS--LLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSS- 60
           MG+MAR L+ S  LLLLLLPCVFVN KRTYIVRMKHHALPSDY THHDWYSAHLQSLSS 
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  --SDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSN 120
             SDSLLYTYTSAYHGFAASLDP+E ELL QSDSVLGVYEDTVYNLHTTRTPGFLGLDS+
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGLVYDIS +DYI FLC+LDYG DHVQAI KRSNITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVG 720
           PGQLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVYEVATTAPSVVKV VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 EKKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL 762
           E+KRYTVTFVASRDAA+TTR+GFGSI W ND HQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Tan0011787 vs. ExPASy TrEMBL
Match: A0A6J1JIF3 (subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3 SV=1)

HSP 1 Score: 1439.5 bits (3725), Expect = 0.0e+00
Identity = 721/766 (94.13%), Postives = 740/766 (96.61%), Query Frame = 0

Query: 1   MGTMARFLIVS--LLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSS- 60
           MG+MAR L+ S  LLLLLLPCVFVN KRTYIVRMKHHALPSDY THHDWYSAHLQSLSS 
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  --SDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSN 120
             SDSLLYTYTSAYHGFAASLDP+E ELL QSDSVLGVYEDTVY LHTTRTPGFLGLDS+
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+SPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVVICDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDIS +DYI FLC+LDYG DHVQAIVKRSNITCS+KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVG 720
           PGQLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVYEVATTAPSVVKV VKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 EKKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL 762
           E+KRYTVTFVASRDAA+TTR+GFGSI W ND HQVRSPVAFAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Tan0011787 vs. ExPASy TrEMBL
Match: A0A5D3CDV9 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001530 PE=3 SV=1)

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 709/762 (93.04%), Postives = 733/762 (96.19%), Query Frame = 0

Query: 1   MGTMARFLIVSLLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSL---SS 60
           M +M RFLI  LLLLLL CVF+N K+TYIV MKHHALPS+Y THHDWYSA LQSL   SS
Sbjct: 1   MDSMPRFLIPFLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSS 60

Query: 61  SDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSNFG 120
           SDSLLYTYTS++HGFAA LD EEVELL QSDSVLGVYEDTVYNLHTTRTPGFLGLDS+FG
Sbjct: 61  SDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFG 120

Query: 121 LWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPSLC 180
           LWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPSLC
Sbjct: 121 LWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSLC 180

Query: 181 NKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYA 240
           NKKLIGARSFSKGYQMASGGGYFRKPRENQS RDQDGHGTHTASTAAGSHV NASLLGYA
Sbjct: 181 NKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYA 240

Query: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRDTI 300
           RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRDTI
Sbjct: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTI 300

Query: 301 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT 360
           AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT
Sbjct: 301 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT 360

Query: 361 GVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGGV 420
           GVSLYSGQGMGNK VALVYNKGSNTSSNMCLPGSL+PA+VRGKVV+CDRGINARVEKGGV
Sbjct: 361 GVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGV 420

Query: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGT 480
           VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS S PTAVLSFGGT
Sbjct: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGT 480

Query: 481 VLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFN 540
           +LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQFN
Sbjct: 481 ILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFN 540

Query: 541 IMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNP 600
           IMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNP
Sbjct: 541 IMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNP 600

Query: 601 WAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFADPG 660
           WAHGAGHVDPHKALSPGL+YDIS +DYIAFLC+LDYG DHVQAIVKRSNITCSRKFADPG
Sbjct: 601 WAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPG 660

Query: 661 QLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGEK 720
           QLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVY+VATTAPSVVKV VKPSKLVFTKVGE+
Sbjct: 661 QLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGER 720

Query: 721 KRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWT 760
           KRYTVTFVASRDAAQTTRFGFGSIVW ND HQVRSPV+FAWT
Sbjct: 721 KRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 762

BLAST of Tan0011787 vs. ExPASy TrEMBL
Match: A0A5A7UKV3 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001560 PE=3 SV=1)

HSP 1 Score: 1417.5 bits (3668), Expect = 0.0e+00
Identity = 711/765 (92.94%), Postives = 735/765 (96.08%), Query Frame = 0

Query: 1   MGTMARFLI-VSLLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSL---S 60
           M +M RFLI   LLLLLL CVF+N K+TYIV MKHHALPS+Y THHDWYSA LQSL   S
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  SSDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSNF 120
           SSDSLLYTYTS++HGFAA LD EEVELL QSDSVLGVYEDTVYNLHTTRTPGFLGLDS+F
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPSL 180
           GLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPRENQS RDQDGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGG 420
           TGVSLYSGQGMGNK VALVYNKGSNTSSNMCLPGSL+PA+VRGKVV+CDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS S PTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGL+YDIS +DYIAFLC+LDYG DHVQAIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGE 720
           GQLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVY+VATTAPSVVKV VKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 KKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL 762
           +KRYTVTFVASRDAAQTTRFGFGSIVW ND HQVRSPV+FAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Tan0011787 vs. ExPASy TrEMBL
Match: A0A1S3BQE8 (subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=1)

HSP 1 Score: 1417.5 bits (3668), Expect = 0.0e+00
Identity = 711/765 (92.94%), Postives = 735/765 (96.08%), Query Frame = 0

Query: 1   MGTMARFLI-VSLLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSL---S 60
           M +M RFLI   LLLLLL CVF+N K+TYIV MKHHALPS+Y THHDWYSA LQSL   S
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  SSDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSNF 120
           SSDSLLYTYTS++HGFAA LD EEVELL QSDSVLGVYEDTVYNLHTTRTPGFLGLDS+F
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPSL 180
           GLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPRENQS RDQDGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMD+AISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGG 420
           TGVSLYSGQGMGNK VALVYNKGSNTSSNMCLPGSL+PA+VRGKVV+CDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS S PTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGL+YDIS +DYIAFLC+LDYG DHVQAIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSIVFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGE 720
           GQLNYPSFS+VFGSKRVVRYTR VTNVGAAGSVY+VATTAPSVVKV VKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 KKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTRL 762
           +KRYTVTFVASRDAAQTTRFGFGSIVW ND HQVRSPV+FAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Tan0011787 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 543/754 (72.02%), Postives = 630/754 (83.55%), Query Frame = 0

Query: 8   LIVSLLLLLLPCVFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLSSSDSLLYTYTS 67
           +I + L LLL       K+TYI+R+ H   P  + THHDWY++    L+S  SLLYTYT+
Sbjct: 13  IITTFLFLLL---HTTAKKTYIIRVNHSDKPESFLTHHDWYTS---QLNSESSLLYTYTT 72

Query: 68  AYHGFAASLDPEEVE-LLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDSNFGLWEGHNTQD 127
           ++HGF+A LD  E + LLS S+S+L ++ED +Y LHTTRTP FLGL+S FG+       D
Sbjct: 73  SFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------HD 132

Query: 128 LDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSPSLCNKKLIGARS 187
           L  +S+ VIIGVLDTG+WPES+SFDDT MPEIPS+W+G CESG DF   LCNKKLIGARS
Sbjct: 133 LGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARS 192

Query: 188 FSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAP 247
           FSKG+QMASGGG+  K RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G ARGMA 
Sbjct: 193 FSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMAT 252

Query: 248 QARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAME 307
           +ARVA YK CW TGCFGSDILA MD+AI DGVDVLSLSLGGGSAPYYRDTIAIGAF+AME
Sbjct: 253 RARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAME 312

Query: 308 KGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQG 367
           +GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG G
Sbjct: 313 RGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVG 372

Query: 368 MGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGGVVRDAGGIGM 427
           MG KP+ LVYNKG+++SSN+CLPGSL+ ++VRGK+V+CDRG+NARVEKG VVRDAGG+GM
Sbjct: 373 MGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGM 432

Query: 428 ILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTVLNVRPSPV 487
           I+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YV+S SKPTA+L F GTVL+V+PSPV
Sbjct: 433 IMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPV 492

Query: 488 VAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSC 547
           VAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSGTSMSC
Sbjct: 493 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 552

Query: 548 PHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVD 607
           PHISGLA LLKAAHPEWSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GHVD
Sbjct: 553 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 612

Query: 608 PHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITCSRKFADPGQLNYPSFSI 667
           P KALSPGLVYDIS ++YI FLC+LDY  DH+ AIVKR ++ CS+KF+DPGQLNYPSFS+
Sbjct: 613 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV 672

Query: 668 VFGSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGEKKRYTVTFVA 727
           +FG KRVVRYTREVTNVGAA SVY+V       V + VKPSKL F  VGEKKRYTVTFV+
Sbjct: 673 LFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 732

Query: 728 SRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAWTR 761
            +  + T +  FGSI W N  H+VRSPVAF+W R
Sbjct: 733 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of Tan0011787 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 813.9 bits (2101), Expect = 1.1e-235
Identity = 421/739 (56.97%), Postives = 527/739 (71.31%), Query Frame = 0

Query: 27  TYIVRMKHHALPSDYPTHHDWYSAHLQSLSSSDSLLYTYTSAYHGFAASLDPEEVELLSQ 86
           TYIV M    +PS +  H +WY + L+S+S S  LLYTY +A HGF+  L  EE + L  
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFSTRLTQEEADSLMT 90

Query: 87  SDSVLGVYEDTVYNLHTTRTPGFLGLDSNFGLWEGHNTQDL--DQASH-DVIIGVLDTGI 146
              V+ V  +  Y LHTTRTP FLGLD         +T DL  +  S+ DV++GVLDTG+
Sbjct: 91  QPGVISVLPEHRYELHTTRTPLFLGLD--------EHTADLFPEAGSYSDVVVGVLDTGV 150

Query: 147 WPESKSFDDTGMPEIPSRWRGACESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKP 206
           WPESKS+ D G   IPS W+G CE+G +F+ SLCN+KLIGAR F++GY+  S  G   + 
Sbjct: 151 WPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE--STMGPIDES 210

Query: 207 RENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFG 266
           +E++SPRD DGHGTHT+STAAGS V  ASLLGYA G ARGMAP+ARVA YK CW  GCF 
Sbjct: 211 KESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFS 270

Query: 267 SDILAGMDQAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKA 326
           SDILA +D+AI+D V+VLS+SLGGG + YYRD +AIGAFAAME+G+ VSCSAGN+GP+ +
Sbjct: 271 SDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSS 330

Query: 327 SLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGNKPVALVY--NKGSN 386
           SL+NVAPWI TVGAGTLDRDFPA   LGNGK FTGVSL+ G+ + +K +  +Y  N  + 
Sbjct: 331 SLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNA 390

Query: 387 TSSNMCLPGSLEPALVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEELVAD 446
           T+ N+C+ G+L P  V+GK+V+CDRGINARV+KG VV+ AGG+GMILANTAA+GEELVAD
Sbjct: 391 TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVAD 450

Query: 447 SHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQ 506
           +HLLPA  VG K GD+IR YV +   PTA +S  GTV+ V+PSPVVAAFSSRGPN +TP 
Sbjct: 451 AHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPN 510

Query: 507 ILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGLAALLKAAHP 566
           ILKPD+I PGVNILAAW+ + GPTGL SD R+ +FNI+SGTSMSCPH+SGLAALLK+ HP
Sbjct: 511 ILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHP 570

Query: 567 EWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALSPGLVYDISI 626
           EWSP+AI+SALMTTAY        L D A G  S P+ HGAGHV P  A +PGL+YD++ 
Sbjct: 571 EWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTT 630

Query: 627 DDYIAFLCTLDYGTDHVQAIVKRSNITCS-RKFADPGQLNYPSFSIVFGSKRVVRYTREV 686
           +DY+ FLC L+Y +  +++ V R N TC   K      LNYPSF++        +YTR V
Sbjct: 631 EDYLGFLCALNYTSPQIRS-VSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTV 690

Query: 687 TNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGEKKRYTVTFVASRDAAQTTRFGFGS 746
           T+VG AG+     T+  + VK+ V+P+ L F +  EKK YTVTF         +   FGS
Sbjct: 691 TSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-SFGS 750

Query: 747 IVWRNDLHQVRSPVAFAWT 760
           I W +  H V SPVA +WT
Sbjct: 751 IEWSDGKHVVGSPVAISWT 757

BLAST of Tan0011787 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 761.5 bits (1965), Expect = 6.3e-220
Identity = 415/777 (53.41%), Postives = 521/777 (67.05%), Query Frame = 0

Query: 4   MARFLIVSLLLL--LLPCVF------VNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSL 63
           MA+  + S+  +  LL C F       +G  +YIV ++    PS + +H++W+ + L+SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 64  SSSD---SLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGL 123
            SS    +LLY+Y+ A HGF+A L P +   L +  SV+ V  D    +HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 124 DSNFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDF 183
             N GLW   N         DVI+GVLDTGIWPE  SF D+G+  IPS W+G CE GPDF
Sbjct: 121 SQNSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 184 SPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANAS 243
             S CN+KLIGAR+F +GY     G      +E++SPRD +GHGTHTASTAAGS VANAS
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 244 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLG-GGSAP 303
           L  YARG A GMA +AR+AAYK CW  GC+ SDILA MDQA++DGV V+SLS+G  GSAP
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 304 -YYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQL 363
            Y+ D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 364 GNGKRFTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPALVRGKVVICDRGINA 423
           G+GK FTG SLY+G+ + +  ++LVY+   +  S +C PG L  +LV GK+V+CDRG NA
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNA 420

Query: 424 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTA 483
           RVEKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++   PTA
Sbjct: 421 RVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTA 480

Query: 484 VLSFGGTVLNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDS 543
            +SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD 
Sbjct: 481 KISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDI 540

Query: 544 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDA 603
           D R+ QFNI+SGTSMSCPH+SGLAALL+ AHP+WSP+AIKSAL+TTAY  +N+   + D 
Sbjct: 541 DPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL 600

Query: 604 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNI-- 663
           A G  SN + HGAGHVDP+KAL+PGLVYDI + +Y+AFLC + Y    +   ++   +  
Sbjct: 601 ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYD 660

Query: 664 TC-SRKFADPGQLNYPSFSIVFGSK-RVVRYTREVTNVGA-AGSVYEVATTAPSVVKVMV 723
            C + K    G LNYPSFS+VF S   VV+Y R V NVG+   +VYEV   +P+ V++ V
Sbjct: 661 ACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDV 720

Query: 724 KPSKLVFTKVGEKKRYTVTF---VASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAW 759
            PSKL F+K      Y VTF   V            FGSI W +  H V+SPVA  W
Sbjct: 721 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Tan0011787 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 760.8 bits (1963), Expect = 1.1e-219
Identity = 401/773 (51.88%), Postives = 519/773 (67.14%), Query Frame = 0

Query: 7   FLIVSLLLLLLPC---VFVNGKRTYIVRMKHHALPSDYPTHHDWYSAHLQSLS------- 66
           F+I+S+ L+ L       ++ K+TY++ M   A+P  Y  H  WYS+ + S++       
Sbjct: 13  FIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEE 72

Query: 67  --SSDSLLYTYTSAYHGFAASLDPEEVELLSQSDSVLGVYEDTVYNLHTTRTPGFLGLDS 126
             +++ +LYTY +A+HG AA L  EE E L + D V+ V  +T Y LHTTR+P FLGL+ 
Sbjct: 73  EGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLER 132

Query: 127 NFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGACESGPDFSP 186
                E           HDV++GVLDTGIWPES+SF+DTGM  +P+ WRGACE+G  F  
Sbjct: 133 Q----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLK 192

Query: 187 SLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSPRDQDGHGTHTASTAAGSHVANASLL 246
             CN+K++GAR F +GY+ A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+L 
Sbjct: 193 RNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLF 252

Query: 247 GYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDQAISDGVDVLSLSLGGGSAPYYR 306
           G+A G ARGMA +ARVAAYK CW  GCF SDIL+ +DQA++DGV VLS+SLGGG + Y R
Sbjct: 253 GFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSR 312

Query: 307 DTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGK 366
           D+++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G  +
Sbjct: 313 DSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMR 372

Query: 367 RFTGVSLYSGQGM--GNKPVALVY---NKGSNTSSNMCLPGSLEPALVRGKVVICDRGIN 426
            F GVSLY G+ +   NK   LVY   N  S   ++ CL G+L+   V GK+VICDRG+ 
Sbjct: 373 TFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVT 432

Query: 427 ARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPT 486
            RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K T
Sbjct: 433 PRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKAT 492

Query: 487 AVLSFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDS 546
           A L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L S
Sbjct: 493 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 552

Query: 547 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDA 606
           D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP+WSP+AIKSALMTTAY  DN    L DA
Sbjct: 553 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDA 612

Query: 607 AGGAFSNPWAHGAGHVDPHKALSPGLVYDISIDDYIAFLCTLDYGTDHVQAIVKRSNITC 666
           +G A S+P+ HGAGH+DP +A  PGLVYDI   +Y  FLCT D     ++   K SN TC
Sbjct: 613 SGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC 672

Query: 667 SRKFA-DPGQLNYPSFSIVFGSK---RVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVK 726
               A +PG LNYP+ S +F      + +   R VTNVG   S Y+V+ +      V V+
Sbjct: 673 KHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQ 732

Query: 727 PSKLVFTKVGEKKRYTVTFVASRDAAQTTRFGFGSIVWRNDLHQVRSPVAFAW 759
           P  L FT   +K  YTVTF   R   +  R  FG +VW++  H+VRSPV   W
Sbjct: 733 PKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Tan0011787 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 732.3 bits (1889), Expect = 4.1e-211
Identity = 389/749 (51.94%), Postives = 508/749 (67.82%), Query Frame = 0

Query: 27  TYIVRMKHHALPSDYPTHHDWYSAHLQSLSSS-DSLLYTYTSAYHGFAASLDPEEVELLS 86
           TYIV + H A PS +PTH  WY++ L SL+SS  S+++TY + +HGF+A L  ++   L 
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLL 86

Query: 87  QSDSVLGVYEDTVYNLHTTRTPGFLGLDS--NFGLWEGHNTQDLDQASHDVIIGVLDTGI 146
               V+ V  + V +LHTTR+P FLGL S    GL E     + D  S D++IGV+DTG+
Sbjct: 87  DHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE-----ESDFGS-DLVIGVIDTGV 146

Query: 147 WPESKSFDDTGMPEIPSRWRGACESGPDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKP 206
           WPE  SFDD G+  +P +W+G C +  DF  S CN+KL+GAR F  GY+  +  G   + 
Sbjct: 147 WPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN--GKMNET 206

Query: 207 RENQSPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFG 266
            E +SPRD DGHGTHTAS +AG +V  AS LGYA G+A GMAP+AR+AAYK CW +GC+ 
Sbjct: 207 TEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYD 266

Query: 267 SDILAGMDQAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKA 326
           SDILA  D A++DGVDV+SLS+GG   PYY D IAIGAF A+++G+FVS SAGN GP   
Sbjct: 267 SDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGAL 326

Query: 327 SLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMG-NKPVALVYN----K 386
           ++ NVAPW+ TVGAGT+DRDFPA V+LGNGK  +GVS+Y G G+   +   LVY      
Sbjct: 327 TVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLG 386

Query: 387 GSNTSSNMCLPGSLEPALVRGKVVICDRGINARVEKGGVVRDAGGIGMILANTAASGEEL 446
           G   SS++CL GSL+P LV+GK+V+CDRGIN+R  KG +VR  GG+GMI+AN    GE L
Sbjct: 387 GDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGL 446

Query: 447 VADSHLLPAVAVGRKTGDLIRQYV------RSVSKPTAVLSFGGTVLNVRPSPVVAAFSS 506
           VAD H+LPA +VG   GD IR+Y+      RS   PTA + F GT L +RP+PVVA+FS+
Sbjct: 447 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 506

Query: 507 RGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCPHISGL 566
           RGPN  TP+ILKPDVI PG+NILAAW + IGP+G+ SD R+T+FNI+SGTSM+CPH+SGL
Sbjct: 507 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 566

Query: 567 AALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDPHKALS 626
           AALLKAAHP+WSP+AI+SAL+TTAYT DN+   + D + G  S+   +G+GHV P KA+ 
Sbjct: 567 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 626

Query: 627 PGLVYDISIDDYIAFLCTLDYGTDHVQAIVKR-SNITCSRKFADPGQLNYPSFSIVF--- 686
           PGLVYDI+  DYI FLC  +Y   ++  I +R ++   +R+    G LNYPSFS+VF   
Sbjct: 627 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQY 686

Query: 687 -GSKRVVRYTREVTNVGAAGSVYEVATTAPSVVKVMVKPSKLVFTKVGEKKRYTVTFVAS 746
             SK    + R VTNVG + SVYE+    P    V V+P KL F +VG+K  + V    +
Sbjct: 687 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 746

Query: 747 --RDAAQTTRFGFGSIVWRNDLHQVRSPV 755
             + +   T    G IVW +    V SP+
Sbjct: 747 EVKLSPGATNVETGHIVWSDGKRNVTSPL 767

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZUF60.0e+0072.02Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
O653511.5e-23456.97Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9LVJ18.9e-21953.41Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... [more]
Q9FLI41.5e-21851.88Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
Q9LUM35.8e-21051.94Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
Match NameE-valueIdentityDescription
KAG6590040.10.0e+0094.38Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022960792.10.0e+0094.26subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-li... [more]
XP_022987083.10.0e+0094.13subtilisin-like protease SBT1.8 [Cucurbita maxima][more]
XP_023516159.10.0e+0094.12subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo][more]
XP_038879224.10.0e+0093.19subtilisin-like protease SBT1.8 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1H8F10.0e+0094.26subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE... [more]
A0A6J1JIF30.0e+0094.13subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3... [more]
A0A5D3CDV90.0e+0093.04Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A5A7UKV30.0e+0092.94Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BQE80.0e+0092.94subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT2G05920.10.0e+0072.02Subtilase family protein [more]
AT5G67360.11.1e-23556.97Subtilase family protein [more]
AT3G14067.16.3e-22053.41Subtilase family protein [more]
AT5G51750.11.1e-21951.88subtilase 1.3 [more]
AT3G14240.14.1e-21151.94Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 540..556
score: 56.45
coord: 131..150
score: 29.87
coord: 211..224
score: 49.97
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 132..582
e-value: 1.0E-49
score: 169.8
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 133..624
e-value: 1.0E-187
score: 626.5
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 101..615
NoneNo IPR availableGENE3D2.60.40.2310coord: 628..758
e-value: 2.5E-36
score: 126.3
NoneNo IPR availableGENE3D3.50.30.30coord: 341..479
e-value: 1.0E-187
score: 626.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 198..222
NoneNo IPR availablePANTHERPTHR10795:SF653CUCUMISIN-RELATEDcoord: 17..758
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 115..611
score: 28.333584
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 347..470
e-value: 2.01439E-40
score: 142.553
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 26..101
IPR003137PA domainPFAMPF02225PAcoord: 381..457
e-value: 8.3E-10
score: 38.6
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 22..102
e-value: 2.4E-17
score: 65.1
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 658..755
e-value: 6.8E-27
score: 93.6
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 27..102
e-value: 3.2E-14
score: 53.3
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 17..758
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 541..551
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 99..577
e-value: 3.5106E-147
score: 430.866

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0011787.1Tan0011787.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity