Tan0011612 (gene) Snake gourd v1

BLAST of Tan0011612 vs. ExPASy Swiss-Prot
Match: Q6NTR1 (eEF1A lysine and N-terminal methyltransferase OS=Xenopus laevis OX=8355 GN=eef1aknmt PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.0e-23
Identity = 111/447 (24.83%), Postives = 193/447 (43.18%), Query Frame = 0

Query: 2   ALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSP-IQPINSPSVAAMFVPSGLETD 61
           AL ++       S+ +S    +     S     ++DSP ++  +S   A   +P G ET+
Sbjct: 251 ALIRNRLNQNQSSQEVSLDLCDGDSRKSRYTFYIVDSPAVRLSHSNHFAIFIIPHGRETE 310

Query: 62  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIATADAIYNRSSHEDSAESPLVVSLQPLF 121
           W+F +E G  QL   S G +RLI+V          D  Y       S  S  V+ L P  
Sbjct: 311 WLFGSEQGRKQLA-GSVGFNRLIIVALHR------DQQYTDMKAIQSELSAKVLELAP-- 370

Query: 122 LALFPKSCFRNGIP---EVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTGM 181
                      G+P   ++P LS  +++ S  +  +      GE++VE+V  + +S    
Sbjct: 371 ----------PGLPDNQQIPFLSAGEDIGSRTIQHRGKSEFSGEYVVEDVRGDGNSS--- 430

Query: 182 FETREFRRRLRFKRMPNLIQTEICIIP---------KANLNLDGVEIQNIEFKPDTRV-- 241
                  RRL F    N++Q+E  ++P         + +       ++++E    TR+  
Sbjct: 431 ------YRRLIFLSNQNVVQSEARLLPISTHIGQKKRKDKKKQQKPVKDLEQPTITRIDK 490

Query: 242 --LVHPYLPPMAASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHL-DFEVVGV 301
             L   +   M + L+L+    +  +    +   L +G+GGG+L  F+  +     V  V
Sbjct: 491 SYLCCEHHKAMISGLALLP---NPGLLPECQASVLVIGLGGGSLSLFIHDYFPGSRVEVV 550

Query: 302 EMDREVLRVAKEHFGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIM 361
           E+D  VL VA   F   ++E + + + D +  +   S +D  + C          YDVIM
Sbjct: 551 EIDPSVLDVASNWFNFCQDERMKVHLADGLVHIN--SLADNGEAC----------YDVIM 610

Query: 362 VDLDSSDVQNGMSAPPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTV 421
            D+DS D   GMS PP  FV + FL N  +IL+ +G+ ++N++  D      +++    V
Sbjct: 611 FDVDSKDPSVGMSCPPPAFVEKMFLQNVHNILNANGVFILNLVCRDTDLRLKVLNVLHEV 654

Query: 422 FDDLFQIDVDNGENFVVIASVCPIRSF 431
           F  ++   +D   N ++       R F
Sbjct: 671 FPLIYAQKIDEEVNEILFCRPNSERKF 654

BLAST of Tan0011612 vs. ExPASy Swiss-Prot
Match: Q8N6R0 (eEF1A lysine and N-terminal methyltransferase OS=Homo sapiens OX=9606 GN=EEF1AKNMT PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 2.2e-23
Identity = 101/412 (24.51%), Postives = 178/412 (43.20%), Query Frame = 0

Query: 35  VLDSP-IQPINSPSVAAMFVPSGLETDWIFCTESGHYQLLFDSPGISRLILVGNQEPLIA 94
           V+DSP ++P      A   +P G ET+W+F  + G  QL   S G  RLI V        
Sbjct: 286 VVDSPTVKPSRDNHFAIFIIPQGRETEWLFGMDEGRKQLA-ASAGFRRLITVALHR---- 345

Query: 95  TADAIYNRSSHEDSAESPLVVSLQPLFLALFPKSCFRNGIP---EVPILSFVDNVISSVV 154
                Y    H  +  S  V+ L P             G+P   +VP LS   ++    V
Sbjct: 346 --GQQYESMDHIQAELSARVMELAPA------------GMPTQQQVPFLSVGGDIGVRTV 405

Query: 155 LEKCIGSSVGEFLVENVEIEKDSGTGMFETREFRRRLRFKRMPNLIQTEICII------- 214
             +      G++++E+V+          + + + RRL F    N++Q+E  ++       
Sbjct: 406 QHQDCSPLSGDYVIEDVQ---------GDDKRYFRRLIFLSNRNVVQSEARLLKDVSHKA 465

Query: 215 ----PKANLNLDGVEIQNIEFKP----DTRVLVHPYLPPMAASLSLIDSSIDQQIQTGHR 274
                K        + +++   P    D   L   +   M A L+L+    + ++     
Sbjct: 466 QKKRKKDRKKQRPADAEDLPAAPGQSIDKSYLCCEHHKAMIAGLALLR---NPELLLEIP 525

Query: 275 PKALCVGVGGGALLTFLATHLDFEVV-GVEMDREVLRVAKEHFGLVENEFLHISIGDAIA 334
              L VG+GGG+L  F+  H     +  VE+D  +L VA + FG  +++ + + I D + 
Sbjct: 526 LALLVVGLGGGSLPLFVHDHFPKSCIDAVEIDPSMLEVATQWFGFSQSDRMKVHIADGLD 585

Query: 335 FLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMSAPPLEFVRRDFLLNARSI 394
           ++ + +   + + C          YDVIM D+DS D   GMS PP  FV + FL   +SI
Sbjct: 586 YIASLAGGGEARPC----------YDVIMFDVDSKDPTLGMSCPPPAFVEQSFLQKVKSI 645

Query: 395 LSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGENFVVIASVCP 427
           L+  G+ ++N++  D    ++++   + VF  L+   ++   N ++   + P
Sbjct: 646 LTPEGVFILNLVCRDLGLKDSVLAGLKAVFPLLYVRRIEGEVNEILFCQLHP 656

BLAST of Tan0011612 vs. ExPASy Swiss-Prot
Match: A5PK19 (eEF1A lysine and N-terminal methyltransferase OS=Bos taurus OX=9913 GN=EEF1AKNMT PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 6.5e-23
Identity = 107/433 (24.71%), Postives = 189/433 (43.65%), Query Frame = 0

Query: 17  ISFSFPNPSHSHSSLRVAVLDSP-IQPINSPSVAAMFVPSGLETDWIFCTESGHYQLLFD 76
           +S    N         + V+DSP ++P      A   +P G ET+W+F  E G  QL   
Sbjct: 268 VSLDLCNGDTGEPRYTLHVVDSPTVKPSRDNHFAIFIIPQGRETEWLFGMEEGRKQLA-A 327

Query: 77  SPGISRLILVGNQEPLIATADAIYNRSSHE--DSAESPLVVSLQPLFLALFPKSCFRNGI 136
           S G  RLI V           A++    +E  DS ++ L   +  L  A  P        
Sbjct: 328 SAGFRRLITV-----------ALHRGQQYEGMDSIQAELSARVMELAPAGMPAQL----- 387

Query: 137 PEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTGMFETREFRRRLRFKRMP 196
            +VP LS   ++   +V  +      G++++E+V+          + + + RRL F    
Sbjct: 388 -QVPFLSVGGDIGVRIVQHQACSPLSGDYVIEDVQ---------GDDKRYFRRLIFLSNR 447

Query: 197 NLIQTEICIIPKAN--------------------LNLDGVEIQNIEFKPDTRVLVHPYLP 256
           N++Q+E  ++   +                     +L   + Q+I    D   L   +  
Sbjct: 448 NVVQSEARLLQDVSHRAQKKRKKDRKKHRPADTPEDLPAAQGQSI----DKSYLCCEHHK 507

Query: 257 PMAASLSLIDSSIDQQIQTGHRPKA-LCVGVGGGALLTFLATHLDFEVV-GVEMDREVLR 316
            M A L+L+ +  +  ++T   P A L VG+GGG+L  F+  H     +  VE+D  +L 
Sbjct: 508 AMIAGLALLKNP-ELLLET---PLALLVVGLGGGSLPLFIHDHFPKSCIHAVEIDPSMLE 567

Query: 317 VAKEHFGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDV 376
           VA + FG  +++ + + I D + F+   ++ + +             YDVIM D+DS D 
Sbjct: 568 VATQWFGFSQSDRMKVHIADGLDFITRLAEEEARP-----------HYDVIMFDVDSKDP 627

Query: 377 QNGMSAPPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQID 425
             GMS PP  FV + FL   +SIL+  G+ ++N++  D    ++++   + VF  L+   
Sbjct: 628 TLGMSCPPPAFVAQLFLQKVKSILTPEGVFILNLVCRDLGLKDSVLAGLKAVFPLLYVRR 654

BLAST of Tan0011612 vs. ExPASy Swiss-Prot
Match: Q29LW1 (eEF1A lysine and N-terminal methyltransferase homolog OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA15401 PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.7e-21
Identity = 106/412 (25.73%), Postives = 171/412 (41.50%), Query Frame = 0

Query: 17  ISFSFPNPSHSHSSLRVAVLD-SPIQPINSPSVAAMFVPSGLETDWIFCTESGHYQLLFD 76
           ++     PS +     + +LD +  + +N    AA  VP G E +W+F T SG  +L   
Sbjct: 268 VTLDLYRPSENTPRYSIYILDQAAARGLN--KYAAFIVPQGREIEWLFGTPSGRKKLQ-A 327

Query: 77  SPGISRLILVGNQEPLIATADAIYNRSSHEDSAESPLVVSLQPLFLALFPKSCFRNGIPE 136
           S    RL +V          D +YN      +     V SL P              I +
Sbjct: 328 SAKFQRLAVV------TLHRDQVYNTLEEVQAELGDTVFSLAP-----------HGHIKQ 387

Query: 137 VPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTGMFETREFRRRLRFKRMPNL 196
           +P LS   +V     L        GEF +E VE    +           RRL F     +
Sbjct: 388 IPYLSLGSDVGKRETLISGFSKISGEFRIEEVEAGGKT----------LRRLIFLSNQFV 447

Query: 197 IQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMAASLSLIDSSIDQQIQTGHR 256
           +Q+E             V+   I+ K + + +   YL   A    L  S   Q   T   
Sbjct: 448 VQSEAL-----------VKTIKIKGKKERKKIDFGYL---ACQHHLYMSVGVQLATTLQN 507

Query: 257 PK------ALCVGVGGGALLTFLATHL-DFEVVGVEMDREVLRVAKEHFGLVENEFLHIS 316
           PK       L +G+GGG L +FL   L    +  VE+D  +L VA+++F L +++  H+ 
Sbjct: 508 PKKDVQKDVLVIGLGGGGLCSFLHAALPQSRITAVEIDPIMLEVAEQYFELKQDKRFHVV 567

Query: 317 IGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMSAPPLEFVRRDFL 376
           I D +AF+E         +C +  +H    +D ++ D+DS D+  GMS PP  F+  D L
Sbjct: 568 IDDGLAFVE---------RCRNEDIH----FDAVLFDVDSKDLSLGMSCPPQGFLAHDVL 622

Query: 377 LNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGENFVV 421
           L+ + I+   G+ ++N++  D+      +   + VF  +    ++   N VV
Sbjct: 628 LHIKEIIGPKGLFMLNLVCRDETLKTEAIANLQKVFPAVCSYKLEEDINEVV 622

BLAST of Tan0011612 vs. ExPASy Swiss-Prot
Match: Q91YR5 (eEF1A lysine and N-terminal methyltransferase OS=Mus musculus OX=10090 GN=EEF1AKNMT PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.0e-20
Identity = 101/410 (24.63%), Postives = 176/410 (42.93%), Query Frame = 0

Query: 35  VLDSP-IQPINSPSVAAMFVPSGLETDWIFCTESGHYQLLFDSPGISRLILVGNQEPLIA 94
           V+D+P ++P      A   +P G ET+W+F  E G  QL   S G  RL+ V        
Sbjct: 286 VVDNPAVKPSRDNHFAIFIIPQGRETEWLFGMEEGRKQLA-ASAGFRRLVTVALHRG--- 345

Query: 95  TADAIYNRSSHEDSAESPLVVSLQPLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEK 154
                  R +  +S ++ L   +  L  A  P         +VP LS   ++    V  +
Sbjct: 346 ------QRYAGMESIQAELSARVMELAPAGLPPQ------QQVPFLSVGGDIGVRTVQHQ 405

Query: 155 CIGSSVGEFLVENVEIEKDSGTGMFETREFRRRLRFKRMPNLIQTEICIIP--------- 214
              +  G++++E+V+          E R + RRL F    N++Q+E  ++          
Sbjct: 406 DHSALSGDYVIEDVQ---------GEDRWYFRRLIFLSNRNVVQSEARLLKDTSHRAQKK 465

Query: 215 -KANLNLDGVEIQNIEFKP------DTRVLVHPYLPPMAASLSLIDSSIDQQIQTGHRPK 274
            K +         + +F P      D   L   +   M A L+L+    + ++       
Sbjct: 466 RKKDRKKQRPADTSEDFPPAPGQSIDKSYLCCEHHKAMVAGLALLR---NPELLLETPLT 525

Query: 275 ALCVGVGGGALLTFLATHL-DFEVVGVEMDREVLRVAKEHFGLVENEFLHISIGDAIAFL 334
            L VG+GGG+L  F+  H     +  VE+D  +L VA + FG  +++ + + I D + ++
Sbjct: 526 LLVVGLGGGSLPLFVHDHFPKSRIDAVEIDPTMLEVATQWFGFSQSDRMKVHIADGLDYI 585

Query: 335 ENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMSAPPLEFVRRDFLLNARSILS 394
            + +        G    H    YDVIM D+DS D   GMS PP  FV + FL   +SIL 
Sbjct: 586 TSLA--------GEAPPH----YDVIMFDVDSKDPTLGMSCPPPAFVDQVFLQKVKSILC 645

Query: 395 EHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGENFVVIASVCP 427
             G+ ++N++  D    ++++   +  F  L+   ++   N ++   + P
Sbjct: 646 HDGVFILNLVCRDVRLKDSVLAGLKAAFPLLYVRRIEGEVNEILFCQLHP 655

BLAST of Tan0011612 vs. NCBI nr
Match: XP_023005401.1 (methyltransferase-like protein 13 [Cucurbita maxima])

HSP 1 Score: 763.8 bits (1971), Expect = 8.3e-217
Identity = 389/464 (83.84%), Postives = 425/464 (91.59%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALDQH FEAL+PSRFISFSFP+P HSHSSLR+AVLDSPIQ  +SPSVA MFVP GLETD
Sbjct: 1   MALDQHMFEALSPSRFISFSFPHPFHSHSSLRLAVLDSPIQFTDSPSVAVMFVPFGLETD 60

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIATAD-AIYNRSSHEDSA---ESPLVVSL 120
           WIF TESGH+QLL+DSPGISRLILVGNQEP+I     ++YNRSS EDSA   ES LVVSL
Sbjct: 61  WIFSTESGHHQLLYDSPGISRLILVGNQEPMITDDSLSVYNRSSDEDSAKSSESRLVVSL 120

Query: 121 QPLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTG 180
           QPLFLALFPKSCF+ GIPEVPILSFVD+VI  VVLE+CIGSSVGEFL+ENVEIE++SG  
Sbjct: 121 QPLFLALFPKSCFKYGIPEVPILSFVDDVICRVVLERCIGSSVGEFLIENVEIERESGKE 180

Query: 181 MFETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMA 240
           MFETREFRRRLRFKRMPNLIQTEI IIP A+L+LDGVEIQNIEFK DTRVLVHPYLPPMA
Sbjct: 181 MFETREFRRRLRFKRMPNLIQTEIRIIPAASLSLDGVEIQNIEFKLDTRVLVHPYLPPMA 240

Query: 241 ASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEH 300
           ASLSLI SSID+QIQTGHRPKALC+GVGGGALLTFLATHLDF+V GVE+D EVLRVA+++
Sbjct: 241 ASLSLIASSIDEQIQTGHRPKALCIGVGGGALLTFLATHLDFKVEGVELDMEVLRVAQQY 300

Query: 301 FGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMS 360
           FGLVENEFLHISIGDA  FLENASKS+KK KCGS GVH+S +YDVIMVDLDSSD +NG+ 
Sbjct: 301 FGLVENEFLHISIGDATEFLENASKSNKKHKCGSFGVHMSSEYDVIMVDLDSSDARNGIG 360

Query: 361 APPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGE 420
           APPL FV RD LL+ARSILSEHGILVMNVIPLDKLFFETL+DKFR+VFDDLF+IDVDNGE
Sbjct: 361 APPLGFVERDVLLSARSILSEHGILVMNVIPLDKLFFETLLDKFRSVFDDLFRIDVDNGE 420

Query: 421 NFVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           NFVV+ASVCPIRSFPH TKNKMNSFLSRLR+LLSGAY+DSIKRI
Sbjct: 421 NFVVVASVCPIRSFPHGTKNKMNSFLSRLRVLLSGAYMDSIKRI 464

BLAST of Tan0011612 vs. NCBI nr
Match: XP_022961507.1 (methyltransferase-like protein 13 [Cucurbita moschata])

HSP 1 Score: 758.1 bits (1956), Expect = 4.5e-215
Identity = 387/464 (83.41%), Postives = 424/464 (91.38%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALDQH FEAL+PSRFISFSFP+P HSHSSLR+AVLDSPIQ  +SPSVA MFVP GLETD
Sbjct: 1   MALDQHMFEALSPSRFISFSFPHPFHSHSSLRLAVLDSPIQFTDSPSVAVMFVPFGLETD 60

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIATAD-AIYNRSSHEDSA---ESPLVVSL 120
           WIF TESGHYQLL+DSPGISRLILVGNQEP++A    ++Y+R S EDSA   ES LVVSL
Sbjct: 61  WIFSTESGHYQLLYDSPGISRLILVGNQEPMVADDSLSVYHRLSDEDSAKSSESRLVVSL 120

Query: 121 QPLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTG 180
           QPLFLALFPKSCF+ GIPEVPILSFVD+VI  VVLE+CIGSSVGEFL+ENVEIE++SG  
Sbjct: 121 QPLFLALFPKSCFKYGIPEVPILSFVDDVICRVVLERCIGSSVGEFLIENVEIERESGKE 180

Query: 181 MFETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMA 240
           +FETREFRRRLRFKRMPNLIQTEI IIP A+L+LDGVEIQNIEFKPDTRVLVHPYLPPMA
Sbjct: 181 IFETREFRRRLRFKRMPNLIQTEIRIIPAASLSLDGVEIQNIEFKPDTRVLVHPYLPPMA 240

Query: 241 ASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEH 300
           ASLSLI SSID+QIQTGHRPKALC+GVGGGALLTFLATHLDF+V GVE+D EVLRVA+++
Sbjct: 241 ASLSLIASSIDEQIQTGHRPKALCIGVGGGALLTFLATHLDFKVEGVELDMEVLRVAQQY 300

Query: 301 FGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMS 360
           FGLVENEFLHISIGDA  FLENASKS+KK+KCGS GVH S +YDVIMVDLDSSD +NG+ 
Sbjct: 301 FGLVENEFLHISIGDATEFLENASKSNKKRKCGSFGVHKSSEYDVIMVDLDSSDARNGIG 360

Query: 361 APPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGE 420
           APPL FV RD LL+ARSILSEHGILVMNVIPLDKLFFETL+DKFR+VFDDLF+IDVDNGE
Sbjct: 361 APPLGFVERDVLLSARSILSEHGILVMNVIPLDKLFFETLLDKFRSVFDDLFRIDVDNGE 420

Query: 421 NFVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           NFVVIASV PIRS PH TKNKMNSFLSRLR+LLSGAY+DSIKRI
Sbjct: 421 NFVVIASVSPIRSLPHGTKNKMNSFLSRLRVLLSGAYMDSIKRI 464

BLAST of Tan0011612 vs. NCBI nr
Match: KAG6596905.1 (eEF1A lysine and N-terminal methyltransferase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030181.1 Methyltransferase-like protein 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 752.3 bits (1941), Expect = 2.5e-213
Identity = 386/464 (83.19%), Postives = 421/464 (90.73%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALDQH FEAL+PSRFISFSFP+P HSHSSLR+AVLDSPIQ  +SPSVA MFVP GLETD
Sbjct: 1   MALDQHMFEALSPSRFISFSFPHPFHSHSSLRLAVLDSPIQFTDSPSVAVMFVPFGLETD 60

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIATAD-AIYNRSSHEDSA---ESPLVVSL 120
           WIF TESGHYQLL+DSPGISRLILVGNQEP++A    ++Y+R S EDSA   ES LVVSL
Sbjct: 61  WIFSTESGHYQLLYDSPGISRLILVGNQEPMVADDSLSVYHRLSDEDSAKSSESRLVVSL 120

Query: 121 QPLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTG 180
           QPLFLALFPKSCF+ GIPEVPILSFVD+VI  VVLE+CIGSSVGEFL+ENVEIE+ SG  
Sbjct: 121 QPLFLALFPKSCFKYGIPEVPILSFVDDVICRVVLERCIGSSVGEFLIENVEIERGSGKE 180

Query: 181 MFETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMA 240
           MFETREFRRRLRFKRMPNLIQTEI IIP A+L+LDGVEIQNIEFK DTRVLVHPYLPPMA
Sbjct: 181 MFETREFRRRLRFKRMPNLIQTEIRIIPAASLSLDGVEIQNIEFKLDTRVLVHPYLPPMA 240

Query: 241 ASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEH 300
           ASLSLI SSID+QIQTGHRPKALC+GVGGGALLTFLATHLDF+V GVE+D EVLRVA+++
Sbjct: 241 ASLSLIASSIDEQIQTGHRPKALCIGVGGGALLTFLATHLDFKVEGVELDVEVLRVAQQY 300

Query: 301 FGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMS 360
           FGLVENEFLHISIGDA  FLENASKS+KK KCGS G H+S +YDVIMVDLDSSD +NG+ 
Sbjct: 301 FGLVENEFLHISIGDATEFLENASKSNKKHKCGSNGAHMSSEYDVIMVDLDSSDARNGIG 360

Query: 361 APPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGE 420
           APPL FV RD LL+ARSILSEHGILVMNVIPLDKLFFETL+DKFR+VFDDLF+IDVDNGE
Sbjct: 361 APPLGFVERDVLLSARSILSEHGILVMNVIPLDKLFFETLLDKFRSVFDDLFRIDVDNGE 420

Query: 421 NFVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           NFVVIASV PIRS PH TKNKMNSFLSRLR+LLSGAY+DSIKRI
Sbjct: 421 NFVVIASVSPIRSLPHGTKNKMNSFLSRLRVLLSGAYMDSIKRI 464

BLAST of Tan0011612 vs. NCBI nr
Match: XP_023539661.1 (methyltransferase-like protein 13 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 746.1 bits (1925), Expect = 1.8e-211
Identity = 384/465 (82.58%), Postives = 422/465 (90.75%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALDQH FEAL+PSRFISFSFP+P HSHSSLR+AVLDSPIQ  +SPSVA MFVP GLETD
Sbjct: 1   MALDQHMFEALSPSRFISFSFPHPFHSHSSLRLAVLDSPIQFTDSPSVAVMFVPFGLETD 60

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIATAD-AIYNRSSHEDSA---ESPLVVSL 120
           WIF TESGHYQLL+DSPGISRLILVGNQEP++A    ++Y RSS EDSA   ES LVVSL
Sbjct: 61  WIFSTESGHYQLLYDSPGISRLILVGNQEPMVADDSLSVYYRSSDEDSAKSSESRLVVSL 120

Query: 121 QPLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTG 180
           QPLFLAL PKSCF+ GIPEVPILSFVD+VI  VVLE+CIGSSVGEFL+ENVEIE++SG  
Sbjct: 121 QPLFLALSPKSCFKYGIPEVPILSFVDDVICRVVLERCIGSSVGEFLIENVEIERESGKE 180

Query: 181 MFETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPD-TRVLVHPYLPPM 240
           MFETREFRRRLRFKRMPNLIQTEI IIP A+L+LDGVEIQNIEFK D +RVLVHPYLPPM
Sbjct: 181 MFETREFRRRLRFKRMPNLIQTEIRIIPAASLSLDGVEIQNIEFKLDSSRVLVHPYLPPM 240

Query: 241 AASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKE 300
           AASLSLI SSID+QIQTGHRPKALC+GVGGGALLTFLATHLDF+V GVE+D EV+RVA++
Sbjct: 241 AASLSLIASSIDEQIQTGHRPKALCIGVGGGALLTFLATHLDFKVEGVELDMEVVRVAQQ 300

Query: 301 HFGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGM 360
           +FGLVENEFLHISIGDA  FLENAS+S+KK KCGS G H+S +YDVIMVDLDSSD +NG+
Sbjct: 301 YFGLVENEFLHISIGDATEFLENASRSNKKHKCGSNGAHMSSEYDVIMVDLDSSDARNGI 360

Query: 361 SAPPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNG 420
            APPL FV RD LL+ARSILSEHGILVMNVIPLDKLFFETL+DKFR+VFDDLF+IDVDNG
Sbjct: 361 GAPPLGFVERDVLLSARSILSEHGILVMNVIPLDKLFFETLLDKFRSVFDDLFRIDVDNG 420

Query: 421 ENFVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           ENFVVIASV PIRSFPH TKNKMNSFLSRLR+LLSGAY+DSIKRI
Sbjct: 421 ENFVVIASVTPIRSFPHGTKNKMNSFLSRLRVLLSGAYMDSIKRI 465

BLAST of Tan0011612 vs. NCBI nr
Match: XP_038906139.1 (eEF1A lysine and N-terminal methyltransferase [Benincasa hispida])

HSP 1 Score: 743.0 bits (1917), Expect = 1.5e-210
Identity = 385/463 (83.15%), Postives = 418/463 (90.28%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALDQH FEALNPSRFISFSFPNP  SHSSLR+AVLDSPI+P  SPSVAAMFVP GLETD
Sbjct: 1   MALDQHMFEALNPSRFISFSFPNPCKSHSSLRIAVLDSPIEPTGSPSVAAMFVPPGLETD 60

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIA-TADAIYNRSSHEDSAE--SPLVVSLQ 120
           WIF TESGHY LLFDSP ISRLILVG+QEP+    +  +YNR   +DSA   S LVVSLQ
Sbjct: 61  WIFSTESGHYHLLFDSPEISRLILVGDQEPVTGHDSFLVYNR---QDSASTWSRLVVSLQ 120

Query: 121 PLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTGM 180
           PLFLALFPKSCF++GIPEVPILSFVDNVI SVVLE+ IGSSVGEFLVENVEIE++S    
Sbjct: 121 PLFLALFPKSCFQHGIPEVPILSFVDNVICSVVLERSIGSSVGEFLVENVEIERES---- 180

Query: 181 FETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMAA 240
            ETREFRRRLRFKRMPNLIQTEI IIP+ANLNLD +EIQNIEFK DTRVLVHPYLPPMAA
Sbjct: 181 LETREFRRRLRFKRMPNLIQTEIRIIPEANLNLDAIEIQNIEFKLDTRVLVHPYLPPMAA 240

Query: 241 SLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEHF 300
           SLSLI SSID+QIQTGHRPKALCVGVGGGALL+FLATHLDFEVVGVEMD EVLR A+ +F
Sbjct: 241 SLSLIASSIDEQIQTGHRPKALCVGVGGGALLSFLATHLDFEVVGVEMDMEVLRAAQHYF 300

Query: 301 GLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMSA 360
           GLVEN FLHISIGDAI FLENASKS+KKQKCGS GVH+S +YDVIMVDLDSSD +NGMS+
Sbjct: 301 GLVENNFLHISIGDAIEFLENASKSNKKQKCGSFGVHMSSEYDVIMVDLDSSDARNGMSS 360

Query: 361 PPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGEN 420
           PPLEFV RD LL+ARS+LSEHGILVMNVIPLDKLFFETLVD+ R+VFDDLFQI+VDNGEN
Sbjct: 361 PPLEFVGRDVLLSARSVLSEHGILVMNVIPLDKLFFETLVDELRSVFDDLFQINVDNGEN 420

Query: 421 FVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           FVVIAS+CPI+SFP+VTKN+MNSF SRLRLLLSGAY+DSIKRI
Sbjct: 421 FVVIASMCPIKSFPNVTKNEMNSFSSRLRLLLSGAYMDSIKRI 456

BLAST of Tan0011612 vs. ExPASy TrEMBL
Match: A0A6J1KUV6 (methyltransferase-like protein 13 OS=Cucurbita maxima OX=3661 GN=LOC111498401 PE=4 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 4.0e-217
Identity = 389/464 (83.84%), Postives = 425/464 (91.59%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALDQH FEAL+PSRFISFSFP+P HSHSSLR+AVLDSPIQ  +SPSVA MFVP GLETD
Sbjct: 1   MALDQHMFEALSPSRFISFSFPHPFHSHSSLRLAVLDSPIQFTDSPSVAVMFVPFGLETD 60

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIATAD-AIYNRSSHEDSA---ESPLVVSL 120
           WIF TESGH+QLL+DSPGISRLILVGNQEP+I     ++YNRSS EDSA   ES LVVSL
Sbjct: 61  WIFSTESGHHQLLYDSPGISRLILVGNQEPMITDDSLSVYNRSSDEDSAKSSESRLVVSL 120

Query: 121 QPLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTG 180
           QPLFLALFPKSCF+ GIPEVPILSFVD+VI  VVLE+CIGSSVGEFL+ENVEIE++SG  
Sbjct: 121 QPLFLALFPKSCFKYGIPEVPILSFVDDVICRVVLERCIGSSVGEFLIENVEIERESGKE 180

Query: 181 MFETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMA 240
           MFETREFRRRLRFKRMPNLIQTEI IIP A+L+LDGVEIQNIEFK DTRVLVHPYLPPMA
Sbjct: 181 MFETREFRRRLRFKRMPNLIQTEIRIIPAASLSLDGVEIQNIEFKLDTRVLVHPYLPPMA 240

Query: 241 ASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEH 300
           ASLSLI SSID+QIQTGHRPKALC+GVGGGALLTFLATHLDF+V GVE+D EVLRVA+++
Sbjct: 241 ASLSLIASSIDEQIQTGHRPKALCIGVGGGALLTFLATHLDFKVEGVELDMEVLRVAQQY 300

Query: 301 FGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMS 360
           FGLVENEFLHISIGDA  FLENASKS+KK KCGS GVH+S +YDVIMVDLDSSD +NG+ 
Sbjct: 301 FGLVENEFLHISIGDATEFLENASKSNKKHKCGSFGVHMSSEYDVIMVDLDSSDARNGIG 360

Query: 361 APPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGE 420
           APPL FV RD LL+ARSILSEHGILVMNVIPLDKLFFETL+DKFR+VFDDLF+IDVDNGE
Sbjct: 361 APPLGFVERDVLLSARSILSEHGILVMNVIPLDKLFFETLLDKFRSVFDDLFRIDVDNGE 420

Query: 421 NFVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           NFVV+ASVCPIRSFPH TKNKMNSFLSRLR+LLSGAY+DSIKRI
Sbjct: 421 NFVVVASVCPIRSFPHGTKNKMNSFLSRLRVLLSGAYMDSIKRI 464

BLAST of Tan0011612 vs. ExPASy TrEMBL
Match: A0A6J1HAD4 (methyltransferase-like protein 13 OS=Cucurbita moschata OX=3662 GN=LOC111459260 PE=4 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.2e-215
Identity = 387/464 (83.41%), Postives = 424/464 (91.38%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALDQH FEAL+PSRFISFSFP+P HSHSSLR+AVLDSPIQ  +SPSVA MFVP GLETD
Sbjct: 1   MALDQHMFEALSPSRFISFSFPHPFHSHSSLRLAVLDSPIQFTDSPSVAVMFVPFGLETD 60

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIATAD-AIYNRSSHEDSA---ESPLVVSL 120
           WIF TESGHYQLL+DSPGISRLILVGNQEP++A    ++Y+R S EDSA   ES LVVSL
Sbjct: 61  WIFSTESGHYQLLYDSPGISRLILVGNQEPMVADDSLSVYHRLSDEDSAKSSESRLVVSL 120

Query: 121 QPLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTG 180
           QPLFLALFPKSCF+ GIPEVPILSFVD+VI  VVLE+CIGSSVGEFL+ENVEIE++SG  
Sbjct: 121 QPLFLALFPKSCFKYGIPEVPILSFVDDVICRVVLERCIGSSVGEFLIENVEIERESGKE 180

Query: 181 MFETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMA 240
           +FETREFRRRLRFKRMPNLIQTEI IIP A+L+LDGVEIQNIEFKPDTRVLVHPYLPPMA
Sbjct: 181 IFETREFRRRLRFKRMPNLIQTEIRIIPAASLSLDGVEIQNIEFKPDTRVLVHPYLPPMA 240

Query: 241 ASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEH 300
           ASLSLI SSID+QIQTGHRPKALC+GVGGGALLTFLATHLDF+V GVE+D EVLRVA+++
Sbjct: 241 ASLSLIASSIDEQIQTGHRPKALCIGVGGGALLTFLATHLDFKVEGVELDMEVLRVAQQY 300

Query: 301 FGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMS 360
           FGLVENEFLHISIGDA  FLENASKS+KK+KCGS GVH S +YDVIMVDLDSSD +NG+ 
Sbjct: 301 FGLVENEFLHISIGDATEFLENASKSNKKRKCGSFGVHKSSEYDVIMVDLDSSDARNGIG 360

Query: 361 APPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGE 420
           APPL FV RD LL+ARSILSEHGILVMNVIPLDKLFFETL+DKFR+VFDDLF+IDVDNGE
Sbjct: 361 APPLGFVERDVLLSARSILSEHGILVMNVIPLDKLFFETLLDKFRSVFDDLFRIDVDNGE 420

Query: 421 NFVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           NFVVIASV PIRS PH TKNKMNSFLSRLR+LLSGAY+DSIKRI
Sbjct: 421 NFVVIASVSPIRSLPHGTKNKMNSFLSRLRVLLSGAYMDSIKRI 464

BLAST of Tan0011612 vs. ExPASy TrEMBL
Match: A0A5A7TD16 (Methyltransferase-like protein 13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00920 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.6e-205
Identity = 374/464 (80.60%), Postives = 414/464 (89.22%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALD H FEALNPSRFISFSFPNP +S SSLR+AVLDSPIQP +SPSVAAMFVP GLETD
Sbjct: 454 MALDVHMFEALNPSRFISFSFPNPCNSRSSLRIAVLDSPIQPTHSPSVAAMFVPPGLETD 513

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIATADA--IYNRSSHEDSAE--SPLVVSL 120
           WIF TESGHY LLFDSPGISRLILVG+QEP +A  D+  IYNR   +DSA   S LVVSL
Sbjct: 514 WIFSTESGHYHLLFDSPGISRLILVGDQEP-VAGLDSLPIYNR---QDSASTWSRLVVSL 573

Query: 121 QPLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTG 180
           QPL LALFPKSCF+NGIPEVPILSFVDNVI  +VLE+CIGSSVGEFLVENVEIE+ S   
Sbjct: 574 QPLLLALFPKSCFKNGIPEVPILSFVDNVIRRLVLERCIGSSVGEFLVENVEIERKS--- 633

Query: 181 MFETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMA 240
            FETREFRRRLRFKRMPNLIQTEI +IP+ANLNLD VEIQN++FKPDTRVLVHPYLPPMA
Sbjct: 634 -FETREFRRRLRFKRMPNLIQTEIRLIPEANLNLDDVEIQNMQFKPDTRVLVHPYLPPMA 693

Query: 241 ASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEH 300
           ASLSLI SSID+QIQTGHRPKALCVGVGGGALL+FLATHLDFEV+GVEMD EVLRVA+++
Sbjct: 694 ASLSLIASSIDKQIQTGHRPKALCVGVGGGALLSFLATHLDFEVMGVEMDMEVLRVAQQY 753

Query: 301 FGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMS 360
           FGLVENEFLHISIGDA  FL+NASKS KKQKC S GVH+S  YDVIM DLDSSD +NG+S
Sbjct: 754 FGLVENEFLHISIGDATEFLQNASKSVKKQKCESFGVHMSSLYDVIMFDLDSSDARNGIS 813

Query: 361 APPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGE 420
           +PPLEFV RD LL+ARS+LSEHGIL++NVIPLDK FF+ L+++FR++FDDLFQIDVDNGE
Sbjct: 814 SPPLEFVGRDVLLSARSVLSEHGILIVNVIPLDKFFFDALINEFRSIFDDLFQIDVDNGE 873

Query: 421 NFVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           NFVVIASVC I+SFP+VTK +MNSF SRLR  LSGAY+DSIKRI
Sbjct: 874 NFVVIASVCSIKSFPNVTKKEMNSFSSRLRSFLSGAYMDSIKRI 909

BLAST of Tan0011612 vs. ExPASy TrEMBL
Match: A0A1S3BKG2 (methyltransferase-like protein 13 OS=Cucumis melo OX=3656 GN=LOC103490826 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.6e-205
Identity = 374/464 (80.60%), Postives = 414/464 (89.22%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALD H FEALNPSRFISFSFPNP +S SSLR+AVLDSPIQP +SPSVAAMFVP GLETD
Sbjct: 1   MALDVHMFEALNPSRFISFSFPNPCNSRSSLRIAVLDSPIQPTHSPSVAAMFVPPGLETD 60

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIATADA--IYNRSSHEDSAE--SPLVVSL 120
           WIF TESGHY LLFDSPGISRLILVG+QEP +A  D+  IYNR   +DSA   S LVVSL
Sbjct: 61  WIFSTESGHYHLLFDSPGISRLILVGDQEP-VAGLDSLPIYNR---QDSASTWSRLVVSL 120

Query: 121 QPLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTG 180
           QPL LALFPKSCF+NGIPEVPILSFVDNVI  +VLE+CIGSSVGEFLVENVEIE+ S   
Sbjct: 121 QPLLLALFPKSCFKNGIPEVPILSFVDNVIRRLVLERCIGSSVGEFLVENVEIERKS--- 180

Query: 181 MFETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMA 240
            FETREFRRRLRFKRMPNLIQTEI +IP+ANLNLD VEIQN++FKPDTRVLVHPYLPPMA
Sbjct: 181 -FETREFRRRLRFKRMPNLIQTEIRLIPEANLNLDDVEIQNMQFKPDTRVLVHPYLPPMA 240

Query: 241 ASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEH 300
           ASLSLI SSID+QIQTGHRPKALCVGVGGGALL+FLATHLDFEV+GVEMD EVLRVA+++
Sbjct: 241 ASLSLIASSIDKQIQTGHRPKALCVGVGGGALLSFLATHLDFEVMGVEMDMEVLRVAQQY 300

Query: 301 FGLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMS 360
           FGLVENEFLHISIGDA  FL+NASKS KKQKC S GVH+S  YDVIM DLDSSD +NG+S
Sbjct: 301 FGLVENEFLHISIGDATEFLQNASKSVKKQKCESFGVHMSSLYDVIMFDLDSSDARNGIS 360

Query: 361 APPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGE 420
           +PPLEFV RD LL+ARS+LSEHGIL++NVIPLDK FF+ L+++FR++FDDLFQIDVDNGE
Sbjct: 361 SPPLEFVGRDVLLSARSVLSEHGILIVNVIPLDKFFFDALINEFRSIFDDLFQIDVDNGE 420

Query: 421 NFVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           NFVVIASVC I+SFP+VTK +MNSF SRLR  LSGAY+DSIKRI
Sbjct: 421 NFVVIASVCSIKSFPNVTKKEMNSFSSRLRSFLSGAYMDSIKRI 456

BLAST of Tan0011612 vs. ExPASy TrEMBL
Match: A0A0A0L5C4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G017010 PE=4 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 9.6e-203
Identity = 371/463 (80.13%), Postives = 411/463 (88.77%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSVAAMFVPSGLETD 60
           MALD H FEALNPSRFI+FSFPNP +S SSLR+AVLDSPI+  +SPSVAAMFVP GLETD
Sbjct: 1   MALDVHMFEALNPSRFITFSFPNPCNSRSSLRIAVLDSPIRLTDSPSVAAMFVPPGLETD 60

Query: 61  WIFCTESGHYQLLFDSPGISRLILVGNQEPLIA-TADAIYNRSSHEDSAE--SPLVVSLQ 120
           WIF TESGHY LLFDSPGISRLILVG+QEP+    +  IYNR   +DSA   S LVVSLQ
Sbjct: 61  WIFSTESGHYHLLFDSPGISRLILVGDQEPVTGHDSLPIYNR---QDSASTWSRLVVSLQ 120

Query: 121 PLFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTGM 180
           PL LALFPKSCF+NGIPEVPILSFVDNVI  VVLE+CIGSSVGEFLVENVEIE++S    
Sbjct: 121 PLLLALFPKSCFKNGIPEVPILSFVDNVIRRVVLERCIGSSVGEFLVENVEIERES---- 180

Query: 181 FETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMAA 240
           FETREFRRRLRFKRMPNLIQTEI +IP+ NLNLDGVEIQNI+FKPDTRVLVHPYLPPM A
Sbjct: 181 FETREFRRRLRFKRMPNLIQTEIRLIPEDNLNLDGVEIQNIQFKPDTRVLVHPYLPPMVA 240

Query: 241 SLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEHF 300
           SLSLI SSID+QIQTGHRPKALCVGVGGGALL+FLATHLDFEV+GVEMD EVLRVA+++F
Sbjct: 241 SLSLIASSIDKQIQTGHRPKALCVGVGGGALLSFLATHLDFEVMGVEMDVEVLRVAQQYF 300

Query: 301 GLVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMSA 360
           GLVENEFLHISIGDA  FL+ ASKS KKQK  S+GVH+S  YDVIM DLDSSD +NGMS+
Sbjct: 301 GLVENEFLHISIGDATEFLQYASKSVKKQKSESLGVHMSSLYDVIMFDLDSSDARNGMSS 360

Query: 361 PPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGEN 420
           PPLEFVRRD LL+ARS+LSEHGIL++NVIPLDK FF+TLV +FR++FDDLFQIDVDNGEN
Sbjct: 361 PPLEFVRRDVLLSARSVLSEHGILIVNVIPLDKFFFDTLVHEFRSIFDDLFQIDVDNGEN 420

Query: 421 FVVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           FVVIASVC I+SFP+VTK ++NSF SRLRL L GAY+DSIKRI
Sbjct: 421 FVVIASVCSIKSFPNVTKEEINSFSSRLRLFLPGAYMDSIKRI 456

BLAST of Tan0011612 vs. TAIR 10
Match: AT5G04610.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 407.5 bits (1046), Expect = 1.4e-113
Identity = 230/462 (49.78%), Postives = 304/462 (65.80%), Query Frame = 0

Query: 1   MALDQHTFEALNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINS--PSVAAMFVPSGLE 60
           MALD   FE  +PSRFISF+ PNP+     LRVAVLDSP+    S  P VAA+ VP   E
Sbjct: 1   MALDVRIFEKTSPSRFISFTIPNPNSPIHLLRVAVLDSPVHSTESSPPRVAAILVPKHRE 60

Query: 61  TDWIFCTESGHYQLLFDSPGISRLILVGNQEPLIATADAIYNRSSHEDSAESPLVVSLQP 120
           +DWIF TESG  QLL + P ISRLIL+G+     +   A+Y+R + ED     L + L+P
Sbjct: 61  SDWIFSTESGQLQLLLNMPEISRLILIGDDHDTDSDLPAVYHRPNGEDDESEKLEIRLKP 120

Query: 121 LFLALFPKSCFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEKDSGTGMF 180
           L +AL PK+  R  I +VP L + DNV+SSV +EKC+G  VGE L+E+VEIE D G    
Sbjct: 121 LVVALLPKTLTREEIDDVPFLIYDDNVVSSVEIEKCVGPFVGEMLIEDVEIEIDDG---- 180

Query: 181 ETREFRRRLRFKRMPNLIQTEICIIPKANLNLDGVEIQNIEFKPDTRVLVHPYLPPMAAS 240
             REFRRRLRFKRMPNL+Q+ I IIP++  N   + +   EFK D   LVHPYL PM AS
Sbjct: 181 -VREFRRRLRFKRMPNLVQSAIKIIPRSCSN-PSLPLMGTEFKLDLTELVHPYLAPMVAS 240

Query: 241 LSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLDFEVVGVEMDREVLRVAKEHFG 300
           LSLI S +   +++  +PKALC+GVGGG LL+FL   L FEV GVE+D EVLR+A+++FG
Sbjct: 241 LSLIGSDVYDHLKS--KPKALCIGVGGGGLLSFLRLQLGFEVTGVEIDPEVLRIARQYFG 300

Query: 301 LVENEFLHISIGDAIAFLENASKSDKKQKCGSIGVHISCKYDVIMVDLDSSDVQNGMSAP 360
           L E     + + D I FL+  SKS     C         ++DV+MVDLDS+D  +GM+AP
Sbjct: 301 L-EESLARVHVEDGIEFLKRLSKS-----CDD-----DARFDVLMVDLDSTDPIHGMTAP 360

Query: 361 PLEFVRRDFLLNARSILSEHGILVMNVIPLDKLFFETLVDKFRTVFDDLFQIDVDNGENF 420
           P+EFV +D LL AR++L   G+ ++NVIP +K F++ L D+FR VF +L++IDV NGENF
Sbjct: 361 PVEFVAKDVLLAARTVLVPSGVFIINVIPPNKTFYQELQDQFRHVFAELYEIDVGNGENF 420

Query: 421 VVIASVCPIRSFPHVTKNKMNSFLSRLRLLLSGAYIDSIKRI 461
           V+IA+V P +S      N+ N     L   +SG Y+D+I++I
Sbjct: 421 VLIATVAPRKS----GFNREN-----LTPAVSGKYLDAIQKI 434

BLAST of Tan0011612 vs. TAIR 10
Match: AT2G31740.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 1.3e-29
Identity = 115/454 (25.33%), Postives = 202/454 (44.49%), Query Frame = 0

Query: 11  LNPSRFISFSFPNPSHSHSSLRVAVLDSPIQPINSPSV---AAMFVPSGLETDWIFCTES 70
           L   R I F+      S+ S R  +LD+  Q    P V       VP     +W+FC+E 
Sbjct: 285 LTEGRRIKFTLGGQG-SNFSYRAVLLDA--QKQTEPFVYYCGVFLVPKTRAHEWLFCSEE 344

Query: 71  GHYQLLFDSPGISRLILVGNQEPLIATADAIYNRSSHEDSAESPLVVSLQPLFLALFPKS 130
           G +Q++ +S   +RLI+V             +  SSH  +    +   L P+   L P++
Sbjct: 345 GQWQVV-ESSQAARLIMV-------------FLDSSHSGATMEDIQNDLSPMVTQLAPRN 404

Query: 131 CFRNGIPEVPILSFVDNVISSVVLEKCIGSSVGEFLVENVEIEK-DSGTGMFETRE--FR 190
              +    +P +   D +     + +      G+ +VE+V  E   S      T      
Sbjct: 405 ---DDEARIPYMMASDGIKKRDTVHEVTSPMTGKVVVEDVVYESAPSNLEDLSTSSDLAF 464

Query: 191 RRLRFKRMPNLIQTEICIIPKANL---------------NLDGVEIQNIE-FKPDTRV-- 250
           RRL FKR   LIQ+E  ++    +                  G + QN E  +P  RV  
Sbjct: 465 RRLVFKRTEGLIQSEALLVEDGEILEQSQKEKTKNVSQSKRKGNKKQNQEPSRPLMRVSH 524

Query: 251 --LVHPYLPPMAASLSLIDSSIDQQIQTGHRPKALCVGVGGGALLTFLATHLD-FEVVGV 310
             L   Y   + +  +L+ S + +    G   K + +G+G G L  FL   L  F +  V
Sbjct: 525 DYLASSYHTGIISGFTLVSSYLKKAESCGTMVKTVVIGLGAGLLPMFLHGCLPFFSIEAV 584

Query: 311 EMDREVLRVAKEHFGLVENEFLHISIGDAIAFL----ENASKSDKKQKCGSIGVHIS--- 370
           E+D  +L V K++FG  +N+ L + I D I F+     + + S++    GS G   +   
Sbjct: 585 ELDPVMLSVGKDYFGFTQNDRLKVHIADGIKFIRDITNSEASSEESSNIGSNGDSTTHNT 644

Query: 371 ----CKYDVIMVDLDSSDVQNGMSAPPLEFVRRDFLLNARSILSEHGILVMNVIPLDKLF 426
               C  D++++D+DS+D   G++ P  +F+   FLL+ +  L +HG+ ++N++   +  
Sbjct: 645 QGGICP-DILIIDVDSADSSGGLTCPASDFIEETFLLSVKQALPQHGLFIVNLVTRSQSV 704