Tan0011293 (gene) Snake gourd v1

Overview
NameTan0011293
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCytochrome p450 76c2
LocationLG07: 5861248 .. 5863095 (-)
RNA-Seq ExpressionTan0011293
SyntenyTan0011293
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAGATGGCTGCTTATGAAGATCTTCTCTCACATCCCCATCTTCTTCTTCTTCCTTTTCTTCTCCTTCTACCTACTCTGTTATTTCTTATTCTCAGACACATCAATTCTCCATCTTCAAAACACCCTCCAGGTCCAAGGCCATGGCCAATTCTCGGAAACCTTCTTCAAATTGGTCAAAATGCTCACATTTCAATCACCCAATTTGCCCAAATCTACGGCCCTCTAATCTCCTTCAAGCTTGGGGCTCAGCGCCTGGTCGTCGCTTCTTCTCCGGCAGCTGCCGCTGCAATTCTCAAGACTCAAGACCGTCTTCTCTCTGCTCGATACATTCCCCACATGATCCCTGATAGAGCTTTACACGACCAATCCTCTCTTGTTTTTTCTCCCGAGTGCAATGATCGTTGGAAGAATTTGAGGTCCATTTGCAAAGCCAATTTGTTTACAGCTAAAGCAATAGAATCCCAAGCCAATTTGAGACGAAGGATGATGAATGACATGGTGGAGTTCTTGCACTCAAAACAGGGATCTGTTGTCGAGATTAAAGATTTTGTGTTCACCACCGTTTTCAACACTCTTTCTAACTTCATCTTCTCGAGGGATCTGCTTGGTTTCGTTGGCGATGGATTCAATGGGATTAAAGAGCCTTTCAAGAAGGCGATGAATTTGAGTTTGACCCCAAATTTAGCTGACTTCTACCCTTTGCTCAGAAGCTTTGATCTTCAGGGTCTTCAAAAGAAAGGAGCCATCTACAAGGGCCAAGTCAACTCTTCGTGGAAAATTCTCATTCAAGAAAGAAGAAAAATCCATTCCCAAAATGGTTCAGCTGACTTCTTGGATATCTTGATCGAAAGTGGATTCACTGATCAACAAATTGATTACTTGATTGTTGTATGTGTGTAAAGAATAATCTCCAATATTAATATCTAACCATACCCTCAAAATTTGAGATAATTAAAAGTTTCAAATGAATTGCAGGAGTTGTTATCAGCAGGAACAGATACAACTACAACGACAGTGGAGTGGGCAATGACAGAGTTATTGAAGAACAGAGACATAACAAACAGAGTGCGAGAGGAGATGAAAAGGGTAATAAATAATGGAACCACAACCACCATTATTGAAGAATCAGAGATCTCTGAGCTTCATTATCTATGGAAATGTGTGAAAGAAACTCTAAGGCTTCATCCACCAGGGCCATTTCTTCTTCCACGAGTAGCTCCAGAAGATTGTGAAGTAATGGGATACTCCATTCCAAAAGATACTCTGATTTTTGTGAATGTTTGGGGGATTGGACGAGACCCATTAGTTTGGGAAGATTCTGAATCGTTCAATCCAGAAAGATTTGGTGGTTGCGATGTGGATTTTAAAGGCTATGATTATCGATATCTACCATTTGGAGGAGGAAGAAGAATTTGCCCTGGACTTCCCATGGCGGTTGTGCAAGTTCCTTTAATCTTAGCCAACTTAATTCATAACTTTGAATGGTTTCTTCCTAATGGTGAAGATTTTTCAAACCTAGATTTAACTGGGAAGCTTTCCATAACATTGCAGAAGGAAGAACCTCTTAAACTCATTCCGAAAAAGAGGCTCTGATTATCATATTCAAGTTTCAGTCCTTTTGATGAAGATCTTCTCTTCAAGAAATATTTTATGTTTATGAAATCAGTTGTTTCTTTCTTCAATGCGTTGTTGAACTTGTAAGTCAATGTTCCCTCACTTTGTACTAATTTTATAGGATTATTAGGACAAGTTGACAGTTTTGAGATGGAGTTGTTCTCGATCTCATCAAACTCAAAAGGTTCCATCGCAAATAAAAGCATAGTCAAGGTGCACAAATGATCATGAG

mRNA sequence

TAAAGATGGCTGCTTATGAAGATCTTCTCTCACATCCCCATCTTCTTCTTCTTCCTTTTCTTCTCCTTCTACCTACTCTGTTATTTCTTATTCTCAGACACATCAATTCTCCATCTTCAAAACACCCTCCAGGTCCAAGGCCATGGCCAATTCTCGGAAACCTTCTTCAAATTGGTCAAAATGCTCACATTTCAATCACCCAATTTGCCCAAATCTACGGCCCTCTAATCTCCTTCAAGCTTGGGGCTCAGCGCCTGGTCGTCGCTTCTTCTCCGGCAGCTGCCGCTGCAATTCTCAAGACTCAAGACCGTCTTCTCTCTGCTCGATACATTCCCCACATGATCCCTGATAGAGCTTTACACGACCAATCCTCTCTTGTTTTTTCTCCCGAGTGCAATGATCGTTGGAAGAATTTGAGGTCCATTTGCAAAGCCAATTTGTTTACAGCTAAAGCAATAGAATCCCAAGCCAATTTGAGACGAAGGATGATGAATGACATGGTGGAGTTCTTGCACTCAAAACAGGGATCTGTTGTCGAGATTAAAGATTTTGTGTTCACCACCGTTTTCAACACTCTTTCTAACTTCATCTTCTCGAGGGATCTGCTTGGTTTCGTTGGCGATGGATTCAATGGGATTAAAGAGCCTTTCAAGAAGGCGATGAATTTGAGTTTGACCCCAAATTTAGCTGACTTCTACCCTTTGCTCAGAAGCTTTGATCTTCAGGGTCTTCAAAAGAAAGGAGCCATCTACAAGGGCCAAGTCAACTCTTCGTGGAAAATTCTCATTCAAGAAAGAAGAAAAATCCATTCCCAAAATGGTTCAGCTGACTTCTTGGATATCTTGATCGAAAGTGGATTCACTGATCAACAAATTGATTACTTGATTGTTGAGTTGTTATCAGCAGGAACAGATACAACTACAACGACAGTGGAGTGGGCAATGACAGAGTTATTGAAGAACAGAGACATAACAAACAGAGTGCGAGAGGAGATGAAAAGGGTAATAAATAATGGAACCACAACCACCATTATTGAAGAATCAGAGATCTCTGAGCTTCATTATCTATGGAAATGTGTGAAAGAAACTCTAAGGCTTCATCCACCAGGGCCATTTCTTCTTCCACGAGTAGCTCCAGAAGATTGTGAAGTAATGGGATACTCCATTCCAAAAGATACTCTGATTTTTGTGAATGTTTGGGGGATTGGACGAGACCCATTAGTTTGGGAAGATTCTGAATCGTTCAATCCAGAAAGATTTGGTGGTTGCGATGTGGATTTTAAAGGCTATGATTATCGATATCTACCATTTGGAGGAGGAAGAAGAATTTGCCCTGGACTTCCCATGGCGGTTGTGCAAGTTCCTTTAATCTTAGCCAACTTAATTCATAACTTTGAATGGTTTCTTCCTAATGGTGAAGATTTTTCAAACCTAGATTTAACTGGGAAGCTTTCCATAACATTGCAGAAGGAAGAACCTCTTAAACTCATTCCGAAAAAGAGGCTCTGATTATCATATTCAAGTTTCAGTCCTTTTGATGAAGATCTTCTCTTCAAGAAATATTTTATGTTTATGAAATCAGTTGTTTCTTTCTTCAATGCGTTGTTGAACTTGTAAGTCAATGTTCCCTCACTTTGTACTAATTTTATAGGATTATTAGGACAAGTTGACAGTTTTGAGATGGAGTTGTTCTCGATCTCATCAAACTCAAAAGGTTCCATCGCAAATAAAAGCATAGTCAAGGTGCACAAATGATCATGAG

Coding sequence (CDS)

ATGGCTGCTTATGAAGATCTTCTCTCACATCCCCATCTTCTTCTTCTTCCTTTTCTTCTCCTTCTACCTACTCTGTTATTTCTTATTCTCAGACACATCAATTCTCCATCTTCAAAACACCCTCCAGGTCCAAGGCCATGGCCAATTCTCGGAAACCTTCTTCAAATTGGTCAAAATGCTCACATTTCAATCACCCAATTTGCCCAAATCTACGGCCCTCTAATCTCCTTCAAGCTTGGGGCTCAGCGCCTGGTCGTCGCTTCTTCTCCGGCAGCTGCCGCTGCAATTCTCAAGACTCAAGACCGTCTTCTCTCTGCTCGATACATTCCCCACATGATCCCTGATAGAGCTTTACACGACCAATCCTCTCTTGTTTTTTCTCCCGAGTGCAATGATCGTTGGAAGAATTTGAGGTCCATTTGCAAAGCCAATTTGTTTACAGCTAAAGCAATAGAATCCCAAGCCAATTTGAGACGAAGGATGATGAATGACATGGTGGAGTTCTTGCACTCAAAACAGGGATCTGTTGTCGAGATTAAAGATTTTGTGTTCACCACCGTTTTCAACACTCTTTCTAACTTCATCTTCTCGAGGGATCTGCTTGGTTTCGTTGGCGATGGATTCAATGGGATTAAAGAGCCTTTCAAGAAGGCGATGAATTTGAGTTTGACCCCAAATTTAGCTGACTTCTACCCTTTGCTCAGAAGCTTTGATCTTCAGGGTCTTCAAAAGAAAGGAGCCATCTACAAGGGCCAAGTCAACTCTTCGTGGAAAATTCTCATTCAAGAAAGAAGAAAAATCCATTCCCAAAATGGTTCAGCTGACTTCTTGGATATCTTGATCGAAAGTGGATTCACTGATCAACAAATTGATTACTTGATTGTTGAGTTGTTATCAGCAGGAACAGATACAACTACAACGACAGTGGAGTGGGCAATGACAGAGTTATTGAAGAACAGAGACATAACAAACAGAGTGCGAGAGGAGATGAAAAGGGTAATAAATAATGGAACCACAACCACCATTATTGAAGAATCAGAGATCTCTGAGCTTCATTATCTATGGAAATGTGTGAAAGAAACTCTAAGGCTTCATCCACCAGGGCCATTTCTTCTTCCACGAGTAGCTCCAGAAGATTGTGAAGTAATGGGATACTCCATTCCAAAAGATACTCTGATTTTTGTGAATGTTTGGGGGATTGGACGAGACCCATTAGTTTGGGAAGATTCTGAATCGTTCAATCCAGAAAGATTTGGTGGTTGCGATGTGGATTTTAAAGGCTATGATTATCGATATCTACCATTTGGAGGAGGAAGAAGAATTTGCCCTGGACTTCCCATGGCGGTTGTGCAAGTTCCTTTAATCTTAGCCAACTTAATTCATAACTTTGAATGGTTTCTTCCTAATGGTGAAGATTTTTCAAACCTAGATTTAACTGGGAAGCTTTCCATAACATTGCAGAAGGAAGAACCTCTTAAACTCATTCCGAAAAAGAGGCTCTGA

Protein sequence

MAAYEDLLSHPHLLLLPFLLLLPTLLFLILRHINSPSSKHPPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLISFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWKNLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFIFSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNSSWKILIQERRKIHSQNGSADFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAMTELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYLPFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLKLIPKKRL
Homology
[Close]
BLAST of Tan0011293 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 5.4e-116
Identity = 224/489 (45.81%), Postives = 308/489 (62.99%), Query Frame = 0 

Query: 13  LLLLPFLLLLPTLLFLILRHINSPSSKHPPGPRPWPILGNLLQIGQNAHISITQFAQIYG 72
           + ++ F  LL   LF ILR  +S     PPGPRP PI+GNLLQ+G   H    + AQ YG
Sbjct: 6   IAIVSFSFLL--FLFFILR--DSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYG 65

Query: 73  PLISFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECND 132
            L S KLG+Q +VVASSPAAAA ILKT D++LS RY+      +  H ++S+V+S ECND
Sbjct: 66  ELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENSIVWS-ECND 125

Query: 133 RWKNLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLS 192
            WK LR +C+  LFT K IESQ+ +R     +MV+FL  K+G VV+I + VF T+ N   
Sbjct: 126 NWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFG 185

Query: 193 NFIFSRDLLGFVG--DGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYK 252
           N IFS+D+        G   +KE   K +++  + N AD++P++   DL G ++  A   
Sbjct: 186 NLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVL 245

Query: 253 GQVNSSWKILIQERRKIHSQNGSADFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVE 312
            Q+   W ++++ERR         DF+D+L+ +G  DQ+I+ L++EL  AGT+T+ +T+E
Sbjct: 246 QQIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIE 305

Query: 313 WAMTELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPF 372
           WA+TEL K   + +++R E+  V+ + T    ++ES++  L YL   VKETLRLHPP P 
Sbjct: 306 WAITELTKKPLVVSKIRLELVNVVGDNT----VKESDLPHLPYLQAFVKETLRLHPPTPL 365

Query: 373 LLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDY 432
           LLPR A E C VM Y+IPK+  I VN W IGRDP  W+D  +F PERF   DVD+KG D+
Sbjct: 366 LLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDF 425

Query: 433 RYLPFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEE 492
             +PFGGGRRICPGLP+A     LI+A L+ NFEW LP G   S L +  K  +TLQK+ 
Sbjct: 426 ELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDP 484

Query: 493 PLKLIPKKR 500
           PL ++ K R
Sbjct: 486 PLLIVLKAR 484

[Close]
BLAST of Tan0011293 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 7.1e-116
Identity = 213/486 (43.83%), Postives = 311/486 (63.99%), Query Frame = 0 

Query: 20  LLLPTLLFLILRHINSPSSKHPPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLISFKL 79
           +++ ++L+L+     S     PPGP+PWPI+GNLLQ+G+  H    + AQ YG + + K+
Sbjct: 10  VIISSILYLLFG--GSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKM 69

Query: 80  GAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWKNLRS 139
           G + +VVAS+ +AA+ ILKT DR+LSARY+      +  H ++S+V+S +C + WKNLR 
Sbjct: 70  GTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRK 129

Query: 140 ICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFIFSRD 199
           +C+  LFT K IESQA++R +   +MVE+L  KQG  V+I + +F T+ N   N IFS++
Sbjct: 130 VCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQN 189

Query: 200 L--LGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNSSW 259
           +  LG    G +  KE   + + L  + N AD++P+L  FDL G +K+ A     + + W
Sbjct: 190 IFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 249

Query: 260 KILIQER---RKIHSQNGSADFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAMT 319
             ++QER   +K+       DF+D+ ++SG  D QI+ L++EL  AGT+T+ +T+EWAMT
Sbjct: 250 GAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 309

Query: 320 ELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLPR 379
           EL KN  IT ++R E++ V+   +    ++ES+   L YL   VKETLRLHPP P LLPR
Sbjct: 310 ELTKNPKITAKIRSEIQTVVGERS----VKESDFPNLPYLEATVKETLRLHPPTPLLLPR 369

Query: 380 VAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYLP 439
            A E C ++ Y+IPKD  I VN WGIGRDP  W D  +F+PERF    VDF+G D+  +P
Sbjct: 370 RALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIP 429

Query: 440 FGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLKL 499
           FG GRRICPGLP+A   + L++A  + N +W LPNG    +L +  K  +TLQKE PL +
Sbjct: 430 FGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFI 487

Query: 500 IPKKRL 501
           +PK R+
Sbjct: 490 VPKSRV 487

[Close]
BLAST of Tan0011293 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 7.1e-116
Identity = 213/486 (43.83%), Postives = 312/486 (64.20%), Query Frame = 0 

Query: 20  LLLPTLLFLILRHINSPSSKHPPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLISFKL 79
           +++ ++L+L+    +S     PPGP+PWPI+GNLLQ+G+  H    + AQ YG + + K+
Sbjct: 11  VIISSILYLLFG--SSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKM 70

Query: 80  GAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWKNLRS 139
           G + +VVAS+ +AA+ ILKT DR+LSARY+      +  H ++S+V+S +C + WKNLR 
Sbjct: 71  GTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRK 130

Query: 140 ICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFIFSRD 199
           +C+  LFT K IESQA++R +   +MVE+L  KQG  V+I + +F T+ N   N IFS++
Sbjct: 131 VCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQN 190

Query: 200 L--LGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNSSW 259
           +  LG    G +  KE   + + L  + N AD++P+L  FDL G +K+ A     + + W
Sbjct: 191 IFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 250

Query: 260 KILIQER---RKIHSQNGSADFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAMT 319
             ++QER   +K+       DF+D+ ++SG  D QI+ L++EL  AGT+T+ +T+EWAMT
Sbjct: 251 GAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 310

Query: 320 ELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLPR 379
           EL KN  IT ++R E++ V+   +    ++ES+   L YL   VKETLRLHPP P LLPR
Sbjct: 311 ELTKNPKITAKLRSELQTVVGERS----VKESDFPNLPYLEATVKETLRLHPPTPLLLPR 370

Query: 380 VAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYLP 439
            A E C ++ Y+IPKD  I VN WGIGRDP  W D  +F+PERF    VDF+G D+  +P
Sbjct: 371 RALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIP 430

Query: 440 FGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLKL 499
           FG GRRICPGLP+A   + L++A  + N +W LPNG    +L +  K  +TLQKE PL +
Sbjct: 431 FGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFI 488

Query: 500 IPKKRL 501
           +PK R+
Sbjct: 491 VPKSRV 488

[Close]
BLAST of Tan0011293 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.8e-114
Identity = 218/484 (45.04%), Postives = 308/484 (63.64%), Query Frame = 0 

Query: 20  LLLPTLLFLILRHINSPSSKHPPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLISFKL 79
           +++ T L+LI R  +S     PPGP+PWPI+GNLLQ+G+  H    Q A+ YG L S KL
Sbjct: 5   VVITTFLYLIFR--DSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKL 64

Query: 80  GAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWKNLRS 139
           G++ +VVAS+P AA+ ILKT DR+LS RY+      +  H ++S+V+S ECN+ WK LR 
Sbjct: 65  GSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNETWKKLRK 124

Query: 140 ICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFIFSRD 199
           +C+  LFT K IESQA +R     +MVE+L    G+ V+I + VF T+ N   N IFS++
Sbjct: 125 VCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQN 184

Query: 200 L--LGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNSSW 259
           +  LG    G   +KE   + + L  + N AD++P L  FDL G  K  A     + S W
Sbjct: 185 IFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 244

Query: 260 KILIQERR--KIHSQNGSADFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAMTE 319
             +++ER+  K H+ +   DF++IL++SG  DQQI+ L++E+  AGT+T+ +T+EWA++E
Sbjct: 245 GAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSE 304

Query: 320 LLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLPRV 379
           L KN  +T  +R E+  V+        ++ES+I  + YL   VKETLRLHP  P LLPR 
Sbjct: 305 LTKNPQVTANMRLELLSVVGKRP----VKESDIPNMPYLQAFVKETLRLHPATPLLLPRR 364

Query: 380 APEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYLPF 439
           A E C+V+ Y+IPK+  I VN WGIGRDP  W D   F+PERF    +DFKG D+  +PF
Sbjct: 365 ALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPF 424

Query: 440 GGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLKLI 499
           G GRRICPG+P+A   + LI+++L+ NF+W  P G D S L +  K  +TLQKE PL ++
Sbjct: 425 GAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIV 480

[Close]
BLAST of Tan0011293 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.2e-101
Identity = 204/493 (41.38%), Postives = 310/493 (62.88%), Query Frame = 0 

Query: 14  LLLPFLLLLPTLLFLILRHINSPSSKH-PPGPRPWPILGNLLQIGQNA----HISITQFA 73
           L +    L+P +L  IL  +  P  K+ PPGP PWP++GNL  +  N     HI++   A
Sbjct: 3   LQIALFSLIPVILVFIL--LLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMA 62

Query: 74  QIYGPLISFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSP 133
           + +GP++   LG Q  V+AS+  AA  ILKT DR+ SAR+I  M      H + SLV+S 
Sbjct: 63  RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVWS- 122

Query: 134 ECNDRWKNLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVF 193
           +C D WK LR I +  +F+ K +++Q+++R + + ++++FL SK+G VV+I  FVF T+ 
Sbjct: 123 DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLL 182

Query: 194 NTLSNFIFSRDLLGFVGDGFN--GIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKG 253
           N L N +FS+D+  + GD  +  GI+   ++ + +   PN+A+FYP L   DLQGL+KK 
Sbjct: 183 NILGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKC 242

Query: 254 AIYKGQVNSSWKILIQERRKIHSQNGSADFLDILIESGFTDQQIDYLIVELLSAGTDTTT 313
                +V   W+  ++E RK +    S D LD+L+ + F D QI+ L +E    G++T++
Sbjct: 243 DERFIRVMKMWEGTVKE-RKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSS 302

Query: 314 TTVEWAMTELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHP 373
            T+EW + EL+K+     +VR+E+  V+  GT+T  I+ES++ +L YL  C+KE +RLHP
Sbjct: 303 ATIEWVIAELIKSPKEMAKVRKELNEVV--GTST--IKESDLPQLPYLQACIKEAMRLHP 362

Query: 374 PGPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFK 433
             PFLLPR A E CEVMGY+IPK++ + VN + IGRDP  W+D  +F PERF   DVDF 
Sbjct: 363 AAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFH 422

Query: 434 GYDYRYLPFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITL 493
           G  Y+++PFG GRR C G+P+A   +PLI+ +L+HN+++ LP G    +L +   LS+TL
Sbjct: 423 GAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTL 482

Query: 494 QKEEPLKLIPKKR 500
             +  L ++PK R
Sbjct: 483 AIDPSLCVVPKAR 485

[Close]
BLAST of Tan0011293 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 769.2 bits (1985), Expect = 2.1e-218
Identity = 382/486 (78.60%), Postives = 421/486 (86.63%), Query Frame = 0 

Query: 18  FLLLLPTLLFLILRHINSPSSKH--PPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLI 77
           FLLLL  L+F+ILRHI+S S +   PPGP+PWPILGNLLQIGQNAHIS+TQF+ IYGPLI
Sbjct: 8   FLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 67

Query: 78  SFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWK 137
           S KLGAQRLVVASSPAAAAAILKTQDRLLSARYI  M PDR LHD  SL+FSPEC DRWK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 138 NLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFI 197
           NLRSIC+A LFTA+AIESQA+LRR+ MN+MVEFL SKQGSVVEIK+ VF TVFNTLSNFI
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 187

Query: 198 FSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNS 257
           FSRDL+GFVGDGFNGIK PFK  ++L L PNLADFYPLLR FDLQGL+KK AIYK QV+S
Sbjct: 188 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 247

Query: 258 SWKILIQERRKIHSQNGSA--DFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAM 317
           SW+ILI+ERR IHSQ  SA  DFLD+LI+SGFTD+QI YLIVE+LSAGTDTTTTTVEWAM
Sbjct: 248 SWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 307

Query: 318 TELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLP 377
            ELLKNRDI NRVREE K+ IN G     I+ES++SELHYLWKCVKETLRLHPP PFLLP
Sbjct: 308 AELLKNRDIMNRVREETKKAINGGA----IDESKLSELHYLWKCVKETLRLHPPAPFLLP 367

Query: 378 RVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYL 437
           R APE CE+MGYS+PK T+IFVNVWGIGRDP +WED +SF PERF GCD D KG+DYR+L
Sbjct: 368 RYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFL 427

Query: 438 PFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLK 497
           PFGGGRRICPGLPMA VQVPLILA LIHNFEW LPN ED S LDL G+L +TLQKEEPLK
Sbjct: 428 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLK 487

Query: 498 LIPKKR 500
           L+ K R
Sbjct: 488 LLSKNR 489

[Close]
BLAST of Tan0011293 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 765.4 bits (1975), Expect = 3.1e-217
Identity = 383/486 (78.81%), Postives = 419/486 (86.21%), Query Frame = 0 

Query: 18  FLLLLPTLLFLILRHINSPSSKH--PPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLI 77
           FLLLL  L+F ILRHI S S +   PPGP PWPILGNLLQIGQNAHIS+TQF+  YGPLI
Sbjct: 8   FLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 67

Query: 78  SFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWK 137
           S KLGAQRLVVASSPAAAAAILKTQDRLLSARYI  M PDR LHD  SL+FSPEC DRWK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 138 NLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFI 197
           NLRSIC+A LFTA+AIESQA+LRR+ MNDMVEFL SKQGSVVEIK+ VF TVFNTLSNFI
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 187

Query: 198 FSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNS 257
           FSRDL+GFVGDGFNGIK  FK  ++L L PNLADFYPLLRSFDLQGL+KK AIYK QV+S
Sbjct: 188 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 247

Query: 258 SWKILIQERRKIHSQNGSA--DFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAM 317
           SW+ILI+ERR IHS++ SA  DFLD+LIESGFTD+QI YLIVE+LSAGTDTTTTTVEWAM
Sbjct: 248 SWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 307

Query: 318 TELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLP 377
            ELLKNRDI NRVREE K+ IN G     I+ES++SELHYLWKCVKETLRLHPP PFLLP
Sbjct: 308 AELLKNRDIMNRVREETKKAINGGA----IDESKLSELHYLWKCVKETLRLHPPAPFLLP 367

Query: 378 RVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYL 437
           R APE CE+MGYS+PKDT+IFVNVWGIGRDP +WED  SF PERF G D D KG+DYR+L
Sbjct: 368 RYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFL 427

Query: 438 PFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLK 497
           PFGGGRRICPGLPMA VQVPLILA LIHNFEW+LPN ED S LDL G+L +TLQKEEPLK
Sbjct: 428 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLK 487

Query: 498 LIPKKR 500
           L+ KKR
Sbjct: 488 LLSKKR 489

[Close]
BLAST of Tan0011293 vs. NCBI nr
Match: XP_022929108.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 756.9 bits (1953), Expect = 1.1e-214
Identity = 375/487 (77.00%), Postives = 424/487 (87.06%), Query Frame = 0 

Query: 18  FLLLLPTLLFLILRHINSPSSK--HPPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLI 77
           FLLLLP L+F IL+HI+S S +   PPGP+PWP+LGNLLQIGQNAHIS+TQF+ IYGPLI
Sbjct: 8   FLLLLP-LIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLI 67

Query: 78  SFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWK 137
           S KLGAQRLVVASSPAAAAAILKTQDRLLSARYI  MIPDRALHD  SLVFSPEC DRWK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWK 127

Query: 138 NLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFI 197
           NLRSIC+A LFTA+AIESQA+LRR+ MN+MVEFL SKQGSVVEIK+FVF TVFNTLSNFI
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFI 187

Query: 198 FSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNS 257
           FSRDL+GFVGDGFNGI+  FK  ++ +L PNLADFYPLLR FDLQGL+KK AIYK Q++S
Sbjct: 188 FSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDS 247

Query: 258 SWKILIQERRKIHSQN--GSADFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAM 317
           SW+ILI+ERR IHS+    ++DFLD+LI+SGFTD+QI+YLIVE+LSAGTDTTTTTVEWAM
Sbjct: 248 SWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWAM 307

Query: 318 TELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLP 377
            ELLKNRDI NRVREE K+ IN       I+ES++SELHYLWKCVKETLRLHPP PFLLP
Sbjct: 308 AELLKNRDIMNRVREETKKAINGDA----IDESKLSELHYLWKCVKETLRLHPPAPFLLP 367

Query: 378 RVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYL 437
           R APE CE+MGYS+PK T+I VNVWGIGRDP +WED +SF PERF  CD D KG+DYR+L
Sbjct: 368 RYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYRFL 427

Query: 438 PFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLK 497
           PFGGGRR+CPGLPMA VQVPLILA LIHNFEW LPN ED S LDL G+L +TLQKEEPLK
Sbjct: 428 PFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLK 487

Query: 498 LIPKKRL 501
           L+PK+R+
Sbjct: 488 LLPKRRI 489

[Close]
BLAST of Tan0011293 vs. NCBI nr
Match: KAG7017133.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 755.4 bits (1949), Expect = 3.2e-214
Identity = 376/485 (77.53%), Postives = 422/485 (87.01%), Query Frame = 0 

Query: 19  LLLLPTLLFLILRHINSPSSKH--PPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLIS 78
           LLLL  L+  IL+HI+S S +   PPGP+PWP+LGNLLQIGQNAHIS+TQF+ IYGPLIS
Sbjct: 10  LLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLIS 69

Query: 79  FKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWKN 138
            KLGAQRLVVASSPAAAAAILKTQDRLLSARYI  MIPDRALHD  SLVFSPEC DRWKN
Sbjct: 70  LKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKN 129

Query: 139 LRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFIF 198
           LRSIC+A LFTA+AIESQA+LRR+ MN+MVEFL SKQGSVVEIK+FVF TVFNTLSNFIF
Sbjct: 130 LRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIF 189

Query: 199 SRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNSS 258
           SRDL+GFVGDGFNGIK  FK  ++L+L PNLADFYPLLR FDLQGL+KK AIYK Q++SS
Sbjct: 190 SRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSS 249

Query: 259 WKILIQERRKIHSQNGSA--DFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAMT 318
           W+ILI+ERR IHS+  SA  DFLD+LI+SG+T++QI+YLIVE+LSAGTD  TTTVEWAM 
Sbjct: 250 WEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD--TTTVEWAMA 309

Query: 319 ELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLPR 378
           ELLKNRDI NRVREE K+ IN G     I+ES++SELHYLWKCVKETLRLHPP PFLLPR
Sbjct: 310 ELLKNRDIMNRVREETKKAINGGA----IDESKLSELHYLWKCVKETLRLHPPAPFLLPR 369

Query: 379 VAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYLP 438
            APE CE+MGYS+PK T+IFVNVWGIGRDP +WED +SF PERF GCD D KG+DYR+LP
Sbjct: 370 YAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLP 429

Query: 439 FGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLKL 498
           FGGGRR+CPGLPMA VQVPLILA LIHNFEW LPN E+ S LDL G+L ITLQKEEPLKL
Sbjct: 430 FGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKEEPLKL 488

Query: 499 IPKKR 500
           +PKKR
Sbjct: 490 LPKKR 488

[Close]
BLAST of Tan0011293 vs. NCBI nr
Match: XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749.6 bits (1934), Expect = 1.8e-212
Identity = 375/486 (77.16%), Postives = 417/486 (85.80%), Query Frame = 0 

Query: 18  FLLLLPTLLFLILRHINSPSSKH--PPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLI 77
           FLLLLP L+F +L+HI+S S +   PPGP PWPILGNLLQIGQNAHIS+TQF+ IYGPLI
Sbjct: 8   FLLLLP-LIFTVLKHISSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHIYGPLI 67

Query: 78  SFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWK 137
           S KLGAQRLVVASSPAAAAAILKTQDRLLSARYI  M PDRALHD  SL+FSPEC DRWK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSLLFSPECGDRWK 127

Query: 138 NLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFI 197
           NLRSIC+A LFTA+AIESQA+LRR+ M DMVEFL SKQGSVVEIK+FVF TVFNTLSN I
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMKDMVEFLQSKQGSVVEIKEFVFITVFNTLSNLI 187

Query: 198 FSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNS 257
           FSRDL+ FVGDGFNGIK PFK  ++L L PNLADFYPLLRSFDLQGL+KK AIYK Q++S
Sbjct: 188 FSRDLVDFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQIDS 247

Query: 258 SWKILIQERRKIHSQNGSA--DFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAM 317
           S +ILI+ERR IHS+  SA  DFLDILIESGFT++QI YLI+E+L AGTDTTTTTVEWAM
Sbjct: 248 SSEILIKERRTIHSRGVSAASDFLDILIESGFTNEQISYLILEVLLAGTDTTTTTVEWAM 307

Query: 318 TELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLP 377
            EL+KNRDI NRVREE K+ +N G     I+ES++SELHYLWKCVKET+RLHPP PFLLP
Sbjct: 308 AELMKNRDIMNRVREETKKALNGGA----IDESKLSELHYLWKCVKETVRLHPPAPFLLP 367

Query: 378 RVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYL 437
           R APE CE+MGYS+PK T+IFVNVWGIGRDP +WED +SF PERF   D D KG+DYR+L
Sbjct: 368 RYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDADLKGHDYRFL 427

Query: 438 PFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLK 497
           PFGGGRR CPGLPMA VQVPLILA LIHNFEW LPN ED S LDL G+L ITLQKEEPLK
Sbjct: 428 PFGGGRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGITLQKEEPLK 487

Query: 498 LIPKKR 500
           L+PKKR
Sbjct: 488 LVPKKR 488

[Close]
BLAST of Tan0011293 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 1.0e-218
Identity = 382/486 (78.60%), Postives = 421/486 (86.63%), Query Frame = 0 

Query: 18  FLLLLPTLLFLILRHINSPSSKH--PPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLI 77
           FLLLL  L+F+ILRHI+S S +   PPGP+PWPILGNLLQIGQNAHIS+TQF+ IYGPLI
Sbjct: 8   FLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 67

Query: 78  SFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWK 137
           S KLGAQRLVVASSPAAAAAILKTQDRLLSARYI  M PDR LHD  SL+FSPEC DRWK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 138 NLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFI 197
           NLRSIC+A LFTA+AIESQA+LRR+ MN+MVEFL SKQGSVVEIK+ VF TVFNTLSNFI
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 187

Query: 198 FSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNS 257
           FSRDL+GFVGDGFNGIK PFK  ++L L PNLADFYPLLR FDLQGL+KK AIYK QV+S
Sbjct: 188 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 247

Query: 258 SWKILIQERRKIHSQNGSA--DFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAM 317
           SW+ILI+ERR IHSQ  SA  DFLD+LI+SGFTD+QI YLIVE+LSAGTDTTTTTVEWAM
Sbjct: 248 SWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 307

Query: 318 TELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLP 377
            ELLKNRDI NRVREE K+ IN G     I+ES++SELHYLWKCVKETLRLHPP PFLLP
Sbjct: 308 AELLKNRDIMNRVREETKKAINGGA----IDESKLSELHYLWKCVKETLRLHPPAPFLLP 367

Query: 378 RVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYL 437
           R APE CE+MGYS+PK T+IFVNVWGIGRDP +WED +SF PERF GCD D KG+DYR+L
Sbjct: 368 RYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFL 427

Query: 438 PFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLK 497
           PFGGGRRICPGLPMA VQVPLILA LIHNFEW LPN ED S LDL G+L +TLQKEEPLK
Sbjct: 428 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLK 487

Query: 498 LIPKKR 500
           L+ K R
Sbjct: 488 LLSKNR 489

[Close]
BLAST of Tan0011293 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.5e-217
Identity = 383/486 (78.81%), Postives = 419/486 (86.21%), Query Frame = 0 

Query: 18  FLLLLPTLLFLILRHINSPSSKH--PPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLI 77
           FLLLL  L+F ILRHI S S +   PPGP PWPILGNLLQIGQNAHIS+TQF+  YGPLI
Sbjct: 8   FLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 67

Query: 78  SFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWK 137
           S KLGAQRLVVASSPAAAAAILKTQDRLLSARYI  M PDR LHD  SL+FSPEC DRWK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 127

Query: 138 NLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFI 197
           NLRSIC+A LFTA+AIESQA+LRR+ MNDMVEFL SKQGSVVEIK+ VF TVFNTLSNFI
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 187

Query: 198 FSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNS 257
           FSRDL+GFVGDGFNGIK  FK  ++L L PNLADFYPLLRSFDLQGL+KK AIYK QV+S
Sbjct: 188 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 247

Query: 258 SWKILIQERRKIHSQNGSA--DFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAM 317
           SW+ILI+ERR IHS++ SA  DFLD+LIESGFTD+QI YLIVE+LSAGTDTTTTTVEWAM
Sbjct: 248 SWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 307

Query: 318 TELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLP 377
            ELLKNRDI NRVREE K+ IN G     I+ES++SELHYLWKCVKETLRLHPP PFLLP
Sbjct: 308 AELLKNRDIMNRVREETKKAINGGA----IDESKLSELHYLWKCVKETLRLHPPAPFLLP 367

Query: 378 RVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYL 437
           R APE CE+MGYS+PKDT+IFVNVWGIGRDP +WED  SF PERF G D D KG+DYR+L
Sbjct: 368 RYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFL 427

Query: 438 PFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLK 497
           PFGGGRRICPGLPMA VQVPLILA LIHNFEW+LPN ED S LDL G+L +TLQKEEPLK
Sbjct: 428 PFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLK 487

Query: 498 LIPKKR 500
           L+ KKR
Sbjct: 488 LLSKKR 489

[Close]
BLAST of Tan0011293 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 5.3e-215
Identity = 375/487 (77.00%), Postives = 424/487 (87.06%), Query Frame = 0 

Query: 18  FLLLLPTLLFLILRHINSPSSK--HPPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLI 77
           FLLLLP L+F IL+HI+S S +   PPGP+PWP+LGNLLQIGQNAHIS+TQF+ IYGPLI
Sbjct: 8   FLLLLP-LIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLI 67

Query: 78  SFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWK 137
           S KLGAQRLVVASSPAAAAAILKTQDRLLSARYI  MIPDRALHD  SLVFSPEC DRWK
Sbjct: 68  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWK 127

Query: 138 NLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNTLSNFI 197
           NLRSIC+A LFTA+AIESQA+LRR+ MN+MVEFL SKQGSVVEIK+FVF TVFNTLSNFI
Sbjct: 128 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFI 187

Query: 198 FSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNS 257
           FSRDL+GFVGDGFNGI+  FK  ++ +L PNLADFYPLLR FDLQGL+KK AIYK Q++S
Sbjct: 188 FSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDS 247

Query: 258 SWKILIQERRKIHSQN--GSADFLDILIESGFTDQQIDYLIVELLSAGTDTTTTTVEWAM 317
           SW+ILI+ERR IHS+    ++DFLD+LI+SGFTD+QI+YLIVE+LSAGTDTTTTTVEWAM
Sbjct: 248 SWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWAM 307

Query: 318 TELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLP 377
            ELLKNRDI NRVREE K+ IN       I+ES++SELHYLWKCVKETLRLHPP PFLLP
Sbjct: 308 AELLKNRDIMNRVREETKKAINGDA----IDESKLSELHYLWKCVKETLRLHPPAPFLLP 367

Query: 378 RVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYL 437
           R APE CE+MGYS+PK T+I VNVWGIGRDP +WED +SF PERF  CD D KG+DYR+L
Sbjct: 368 RYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYRFL 427

Query: 438 PFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLK 497
           PFGGGRR+CPGLPMA VQVPLILA LIHNFEW LPN ED S LDL G+L +TLQKEEPLK
Sbjct: 428 PFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLK 487

Query: 498 LIPKKRL 501
           L+PK+R+
Sbjct: 488 LLPKRRI 489

[Close]
BLAST of Tan0011293 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 4.2e-212
Identity = 377/496 (76.01%), Postives = 422/496 (85.08%), Query Frame = 0 

Query: 13  LLLLPFLLLLPTLLFLILRHINSPSSK--HPPGPRPWPILGNLLQIGQNAHISITQFAQI 72
           +LL   LLLLP  +F+IL+HI SPSSK   PPGP PWPILGNLLQIGQNAHISITQFA I
Sbjct: 8   ILLPSLLLLLP--IFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANI 67

Query: 73  YGPLISFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPEC 132
           YGPLIS KLGAQRL+VASSPAAA A+LKTQDRLLSARYI  M PDRALHDQ SLVFSPEC
Sbjct: 68  YGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPEC 127

Query: 133 NDRWKNLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNT 192
            D+WKNLRS+CK NLFTAKAIESQA LR + M ++V+FL SKQGSVVE+KDFVFT+VFNT
Sbjct: 128 GDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNT 187

Query: 193 LSNFIFSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYK 252
           LSN IFS DLL FVGDGF GIK PFKK M+L LTPNLADFYPLLR FDLQGL+ K  IYK
Sbjct: 188 LSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYK 247

Query: 253 GQVNSSWKILIQERRKIHSQNGSA--DFLDILIESGFTDQQIDYLIVELLSAGTDTTTTT 312
            +V+S W ILI+ERR+IH Q GSA  DFLD+LI+SGF DQQI+ LI+ELLSAGTDTTTTT
Sbjct: 248 NEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTT 307

Query: 313 VEWAMTELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPG 372
           VEWAM+E+LK++DI N+VREEMK+V         I+ES ISEL YLW+CVKETLRLHPP 
Sbjct: 308 VEWAMSEILKDKDILNKVREEMKKVTKENRP---IDESNISELQYLWRCVKETLRLHPPA 367

Query: 373 PFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERF----GGCDVD 432
           PFLLPR+AP DCEVMGYSIPKD+++FVNVWGIGRDP VWEDS++FNP+RF    G  +VD
Sbjct: 368 PFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVD 427

Query: 433 FKGYDYRYLPFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSI 492
           FKGYDYRYLPFGGGRRICPGLPMA+VQVPLILA LIHNFEW LPN ED S LDL G+L +
Sbjct: 428 FKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGV 487

Query: 493 TLQKEEPLKLIPKKRL 501
           TLQK E LKLIPK+R+
Sbjct: 488 TLQKYETLKLIPKRRV 498

[Close]
BLAST of Tan0011293 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 7.2e-212
Identity = 376/496 (75.81%), Postives = 422/496 (85.08%), Query Frame = 0 

Query: 13  LLLLPFLLLLPTLLFLILRHINSPSSK--HPPGPRPWPILGNLLQIGQNAHISITQFAQI 72
           +LL   LLLLP  +F+IL+HI SPSSK   PPGP PWPILGNLLQIGQNAHISITQFA I
Sbjct: 8   ILLPSLLLLLP--IFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANI 67

Query: 73  YGPLISFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSSLVFSPEC 132
           YGPLIS KLGAQRL+VASSPAAA A+LKTQDRLLSARYI  M PDRALHDQ SLVFSPEC
Sbjct: 68  YGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPEC 127

Query: 133 NDRWKNLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHSKQGSVVEIKDFVFTTVFNT 192
            D+WKNLRS+CK NLFTAKAIESQA LR + M ++V+FL SKQGSVVE+KDFVFT+VFNT
Sbjct: 128 GDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNT 187

Query: 193 LSNFIFSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYK 252
           LSN IFS DL+ FVGDGF GIK PFKK M+L LTPNLADFYPLLR FDLQGL+ K  IYK
Sbjct: 188 LSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYK 247

Query: 253 GQVNSSWKILIQERRKIHSQNGSA--DFLDILIESGFTDQQIDYLIVELLSAGTDTTTTT 312
            +V+S W ILI+ERR+IH Q GSA  DFLD+LI+SGF DQQI+ LI+ELLSAGTDTTTTT
Sbjct: 248 NEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTT 307

Query: 313 VEWAMTELLKNRDITNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPG 372
           VEWAM+E+LK++DI N+VREEMK+V         I+ES ISEL YLW+CVKETLRLHPP 
Sbjct: 308 VEWAMSEILKDKDILNKVREEMKKVTKENRP---IDESNISELQYLWRCVKETLRLHPPA 367

Query: 373 PFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERF----GGCDVD 432
           PFLLPR+AP DCEVMGYSIPKD+++FVNVWGIGRDP VWEDS++FNP+RF    G  +VD
Sbjct: 368 PFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVD 427

Query: 433 FKGYDYRYLPFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSI 492
           FKGYDYRYLPFGGGRRICPGLPMA+VQVPLILA LIHNFEW LPN ED S LDL G+L +
Sbjct: 428 FKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGV 487

Query: 493 TLQKEEPLKLIPKKRL 501
           TLQK E LKLIPK+R+
Sbjct: 488 TLQKYETLKLIPKRRV 498

[Close]
BLAST of Tan0011293 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 346.7 bits (888), Expect = 3.2e-95
Identity = 191/477 (40.04%), Postives = 284/477 (59.54%), Query Frame = 0 

Query: 41  PPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLISFKLGAQRLVVASSPAAAAAILKTQ 100
           PPGP   PI+GN+  +G+N H S    ++ YGP++S K G+   VV +SP AA  +L+T 
Sbjct: 39  PPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTY 98

Query: 101 DRLLSARYIPHMIPDRAL-HDQSSLVFSPECNDRWKNLRSICKANLFTAKAIESQANLRR 160
           D++LS+R   + I  R++ HD+ S+V+ P  + RW+ LR +    LF+ + IE+   LR 
Sbjct: 99  DQILSSRTPTNSI--RSINHDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRE 158

Query: 161 RMMNDMVEFL--HSKQGSVVEIKDFVFTTVFNTLSNFIFSRDLLGFVGDGFNGIKEPFKK 220
             + ++V F+   S++   V+I    F T  N +SN +FS DL  +  +     ++    
Sbjct: 159 NKVKELVSFMSESSEREEAVDISRATFITALNIISNILFSVDLGNYDSNKSGVFQDTVIG 218

Query: 221 AMNLSLTPNLADFYPLLRSFDLQGLQKK--------GAIYKGQVNSSWKILIQERRKIHS 280
            M     P+ A+F+P L   DLQG +K           +++G +++  K+  +  R  +S
Sbjct: 219 VMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFKVFRGFIDA--KLAEKSLRDTNS 278

Query: 281 QN-GSADFLDILI------ESGFTDQQIDYLIVELLSAGTDTTTTTVEWAMTELLKNRDI 340
           ++    DF+D+L+      E+      I +L+++L  AGTDT ++TVEWAM ELL+N + 
Sbjct: 279 KDVRERDFVDVLLDLTEGDEAELNTNDIVHLLLDLFGAGTDTNSSTVEWAMAELLRNPET 338

Query: 341 TNRVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLPRVAPEDCEV 400
             + + E+  VI       ++EES+IS L YL   VKET RLHP  P L+PR A  D EV
Sbjct: 339 MVKAQAEIDCVIGQ---KGVVEESDISALPYLQAVVKETFRLHPAAPLLVPRKAESDVEV 398

Query: 401 MGYSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYLPFGGGRRIC 460
           +G+ +PKDT +FVNVW IGRDP VWE+S  F PERF G D+D +G DY   PFG GRRIC
Sbjct: 399 LGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKDIDLRGRDYELTPFGAGRRIC 458

Query: 461 PGLPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLKLIPKKR 500
           PGLP+AV  VPL+LA+L+++F+W LPNG    +LD+     +TL K  PL  +P K+
Sbjct: 459 PGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLTLHKTNPLHAVPVKK 508

[Close]
BLAST of Tan0011293 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 343.6 bits (880), Expect = 2.7e-94
Identity = 188/475 (39.58%), Postives = 284/475 (59.79%), Query Frame = 0 

Query: 41  PPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLISFKLGAQRLVVASSPAAAAAILKTQ 100
           PPGP   PI+GN+ Q+G+N H S    A+IYGP++S K G    VV +SP AA  +L+T 
Sbjct: 39  PPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRTH 98

Query: 101 DRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWKNLRSICKANLFTAKAIESQANLRRR 160
           D++LS R     I     H++ S+++ P  + RW+ LR +    +F+ +  E+   LR +
Sbjct: 99  DQILSGRKSNDSIRCFG-HEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEATKALRMK 158

Query: 161 MMNDMVEFLH--SKQGSVVEIKDFVFTTVFNTLSNFIFSRDLLGFVGDGFNGIKEPFKKA 220
            + ++V F++  S++   V+I    +TTV N +SN +FS DL  +     N  ++    A
Sbjct: 159 KVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVDLGSYDSKKSNEFQDTVIGA 218

Query: 221 MNLSLTPNLADFYPLLRSFDLQGLQK--KG------AIYKGQVNSSWKILIQERRKIHSQ 280
           M  +  P+ A+++P +   DLQG +K  +G       +++G +++  KI  +        
Sbjct: 219 MEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGFMDA--KIAEKSLGNYSKD 278

Query: 281 NGSADFLDILI------ESGFTDQQIDYLIVELLSAGTDTTTTTVEWAMTELLKNRDITN 340
             + DFLD L+      E+   +  I++L++++ +AGTDT+++T+EWAM ELL+N     
Sbjct: 279 VSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTDTSSSTLEWAMAELLRNPKTMV 338

Query: 341 RVREEMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLPRVAPEDCEVMG 400
           + + EM RV+      ++++ES+IS L YL   VKET RLHP  P L+PR A  D EV+G
Sbjct: 339 KAQAEMDRVLGQ---NSVVQESDISGLPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLG 398

Query: 401 YSIPKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYLPFGGGRRICPG 460
           + +PKDT + VNVW IGRDP VWE+   F PERF G D+D KG DY   PFGGGRRICPG
Sbjct: 399 FMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFMGKDIDVKGRDYELTPFGGGRRICPG 458

Query: 461 LPMAVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLKLIPKKR 500
           LP+AV  V L+LA+L+++F+W LPNG    +LD+     ITL +   L  IP K+
Sbjct: 459 LPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLHRTNTLYAIPVKK 507

[Close]
BLAST of Tan0011293 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 337.4 bits (864), Expect = 1.9e-92
Identity = 197/514 (38.33%), Postives = 304/514 (59.14%), Query Frame = 0 

Query: 6   DLLSHPHLLLLPFLLLLPTLLFLILR--HINSPSSKHPPGPRPWPILGNLLQIGQNAHIS 65
           D++S   LLLL   +L   L+F   R   I+  ++  PPGP   PI+GN+  +G++ H S
Sbjct: 2   DIISGQALLLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRS 61

Query: 66  ITQFAQIYGPLISFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRAL-HDQS 125
             + ++ YGP++S KLG+   VV +SP AA  +L+T D++LSAR   + +  R++ H  +
Sbjct: 62  FAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAV--RSINHQDA 121

Query: 126 SLVFSPECNDRWKNLRSICKANLFTAKAIESQANLRRRMMNDMVEFL--HSKQGSVVEIK 185
           SLV+ P  + RW+ LR +    L + + IE+   LR   + ++V F+   S +   V+I 
Sbjct: 122 SLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDIS 181

Query: 186 DFVFTTVFNTLSNFIFSRDLLGF-VGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDL 245
              F T  N +SN +FS DL  +      NG+++     M+ + TP+ A+++P LR  DL
Sbjct: 182 RVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDL 241

Query: 246 QG--------LQKKGAIYKGQVNSSWKILIQERRKIHSQNGSADFLDILI-----ESGFT 305
           QG         ++   +++G +++  KI  +  +         DF+D L+     ES  +
Sbjct: 242 QGNVKTFKVCTERLVRVFRGFIDA--KIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELS 301

Query: 306 DQQIDYLIVELLSAGTDTTTTTVEWAMTELLKNRDITNRVREEMKRVI-NNGTTTTIIEE 365
              I++L++++ +AGTDT+++T+EWAMTELLKN     + + E+  VI  NG    I+EE
Sbjct: 302 ISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNG----IVEE 361

Query: 366 SEISELHYLWKCVKETLRLHPPGPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPL 425
           S+IS+L YL   VKET RLH P P L+PR A  D E++G+ + KDT + VNVW IGRDP 
Sbjct: 362 SDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPS 421

Query: 426 VWEDSESFNPERFGGCDVDFKGYDYRYLPFGGGRRICPGLPMAVVQVPLILANLIHNFEW 485
           VW++   F PERF G D+D +G DY   PFG GRRICPG+P+A+  V L+LA+L+++F+W
Sbjct: 422 VWDNPSQFEPERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDW 481

Query: 486 FLPNGEDFSNLDLTGKLSITLQKEEPLKLIPKKR 500
            LP G    +LD+     +TL K  PL  +P K+
Sbjct: 482 KLPKGVLSEDLDMDETFGLTLHKTNPLHAVPVKK 507

[Close]
BLAST of Tan0011293 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 337.0 bits (863), Expect = 2.5e-92
Identity = 198/511 (38.75%), Postives = 288/511 (56.36%), Query Frame = 0 

Query: 6   DLLSHPHLLLLPFLLLLPTLLFLILRHINSPS--SKHPPGPRPWPILGNLLQIGQNAHIS 65
           D++S   L L+   +L   L F   R   SP   SK PPGP   PI+GN+  +G+N H S
Sbjct: 2   DIISGQPLFLIFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHS 61

Query: 66  ITQFAQIYGPLISFKLGAQRLVVASSPAAAAAILKTQDRLLSARYIPHMIPDRALHDQSS 125
            T  ++ YGP++S KLG    VV +S  A   +LKT D++LS RYI         H+ S 
Sbjct: 62  FTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNNHHEFSV 121

Query: 126 LVFSPECNDRWKNLRSICKANLFTAKAIESQANLRRRMMNDMVEFLHS--KQGSVVEIKD 185
               P  + R++ LR +    LF+ + I++   LR + + ++V FL    ++   V+I  
Sbjct: 122 GWIHPS-SSRFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISH 181

Query: 186 FVFTTVFNTLSNFIFSRDLLGFVGDGFNGIKEPFKKAMNLSLTPNLADFYPLLRSFDLQG 245
             F T  N +SN +FS +L  +     +  +E           P+LA+F+P +R  DLQG
Sbjct: 182 VSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQG 241

Query: 246 LQKKGAIYKGQVNSSW------KILIQERRKIHSQNGSADFLDILI------ESGFTDQQ 305
             KK     G++   +      +I+ +  R +     S DFLD+LI      E+     +
Sbjct: 242 NSKKMRESSGRLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEINIDE 301

Query: 306 IDYLIVELLSAGTDTTTTTVEWAMTELLKNRDITNRVREEMKRVI-NNGTTTTIIEESEI 365
           I++L++++  AGTDT ++TVEWAM ELL N     +V++E+  VI  NG      +ES+I
Sbjct: 302 IEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGD----FQESDI 361

Query: 366 SELHYLWKCVKETLRLHPPGPFLLPRVAPEDCEVMGYSIPKDTLIFVNVWGIGRDPLVWE 425
           S+L YL   VKET RLHP  PFLL R A  + E++G+++ KD+ + VNVW IGRDPLVWE
Sbjct: 362 SKLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWE 421

Query: 426 DSESFNPERFGGCDVDFKGYDYRYLPFGGGRRICPGLPMAVVQVPLILANLIHNFEWFLP 485
           +   F PERF G ++D KG DY   PFG GRRICPGLP+A+  V L+LA+L++ FEW LP
Sbjct: 422 NPTHFEPERFLGKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLP 481

Query: 486 NGEDFSNLDLTGKLSITLQKEEPLKLIPKKR 500
           NG    +LD+     +T+ K  PL  +P K+
Sbjct: 482 NGVGSEDLDMEETFGLTVHKTNPLLAVPLKK 507

[Close]
BLAST of Tan0011293 vs. TAIR 10
Match: AT3G61040.1 (cytochrome P450, family 76, subfamily C, polypeptide 7 )

HSP 1 Score: 316.6 bits (810), Expect = 3.5e-86
Identity = 180/472 (38.14%), Postives = 275/472 (58.26%), Query Frame = 0 

Query: 38  SKHPPGPRPWPILGNLLQIGQNAHISITQFAQIYGPLISFKLGAQRLVVASSPAAAAAIL 97
           +K+PPGP    +L N+LQ  +  H S+   ++IYG ++SFKLG    VV SSP  A  +L
Sbjct: 31  AKNPPGPSKLSLLRNILQTVEKPHRSLADLSRIYGSVMSFKLGCLTTVVISSPETAKEVL 90

Query: 98  KTQDRLLSARYIPHMIPDRALHDQSSLVFSPECNDRWKNLRSICKANLFTAKAIESQANL 157
           KT D +LS R     +     H+ S L   P    RW+ LR I +  LF+ + +E+ + +
Sbjct: 91  KTHDHVLSYRVSSDPVRAAGHHELSLLWIPPLA--RWRFLRKITRNQLFSTQRLEATSAI 150

Query: 158 RRRMMNDMVEFLHS--KQGSVVEIKDFVFTTVFNTLSNFIFSRDLLGF-VGDGFNGIKEP 217
           R R + +++ F++   ++   V I    F T  N +SN +FS +L  F     F+  +  
Sbjct: 151 RTRKVQELMNFVNKCCERREAVNISRASFITSLNIISNALFSTNLANFDDSKTFHDFQNV 210

Query: 218 FKKAMNLSLTPNLADFYPLLRSFDLQGLQKKGAIYKGQVNSSWKILIQERRKIHSQNGSA 277
             + M +S  PNLADF+P L   DLQG +K+  +   ++   ++  I  +R   S+N + 
Sbjct: 211 VIRMMEISGKPNLADFFPFLGFLDLQGARKEARLLMHKLFRVFQGFIDTKRSSTSRNNN- 270

Query: 278 DFLDILI------ESGFTDQQIDYLIVELLSAGTDTTTTTVEWAMTELLKNRDITNRVRE 337
           D LD L+      ES   D  I +L+++L  AG DT+++ VEWAM ELL+N  +  +V+E
Sbjct: 271 DMLDSLLDIAHKKESELDDNNIKHLLLDLFLAGVDTSSSAVEWAMAELLRNPKMIVKVQE 330

Query: 338 EMKRVINNGTTTTIIEESEISELHYLWKCVKETLRLHPPGPFLLPRVA-PEDCEVMGYSI 397
           E+++VI    T   +++ +I +L YL   VKE+LRLHPP PFL+PR +  +D ++  + I
Sbjct: 331 EIRQVIGLKGT---VQDLDIVKLPYLQAVVKESLRLHPPAPFLVPRKSESDDVQIFEFLI 390

Query: 398 PKDTLIFVNVWGIGRDPLVWEDSESFNPERFGGCDVDFKGYDYRYLPFGGGRRICPGLPM 457
           PK+T + VNVW IGRDP VW++   F PERF G  +D KG  +  +PFG GRRICPG+P+
Sbjct: 391 PKNTQVLVNVWAIGRDPNVWKNPTQFEPERFLGRGIDVKGNHFELIPFGAGRRICPGMPL 450

Query: 458 AVVQVPLILANLIHNFEWFLPNGEDFSNLDLTGKLSITLQKEEPLKLIPKKR 500
           A   + L+LA+L++ F+W   NG    N+D+      TL K EPL ++P K+
Sbjct: 451 AFRIMHLVLASLLYGFDWEYQNGVVPENVDMNEAFGATLHKAEPLCIVPIKK 496

The following BLAST results are available for this feature:
BLAST of Tan0011293 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FXW45.4e-11645.81Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... [more]
O648997.1e-11643.83(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... [more]
O649007.1e-11643.83(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... [more]
Q9SP061.8e-11445.04(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... [more]
A8CDR52.2e-10141.38Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1[more]
BLAST of Tan0011293 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022928791.12.1e-21878.60probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata][more]
XP_022970204.13.1e-21778.81probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima][more]
XP_022929108.11.1e-21477.00probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata][more]
KAG7017133.13.2e-21477.53(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma... [more]
XP_023551257.11.8e-21277.16probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. ... [more]
BLAST of Tan0011293 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1ELA61.0e-21878.60probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A6J1I4V71.5e-21778.81probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... [more]
A0A6J1ER525.3e-21577.00probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A1S3CT144.2e-21276.01probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... [more]
A0A5A7UIU37.2e-21275.81Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... [more]
BLAST of Tan0011293 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G45570.13.2e-9540.04cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
AT2G45550.12.7e-9439.58cytochrome P450, family 76, subfamily C, polypeptide 4 [more]
AT2G45560.11.9e-9238.33cytochrome P450, family 76, subfamily C, polypeptide 1 [more]
AT1G33720.12.5e-9238.75cytochrome P450, family 76, subfamily C, polypeptide 6 [more]
AT3G61040.13.5e-8638.14cytochrome P450, family 76, subfamily C, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 289..306
score: 31.54
coord: 432..442
score: 55.82
coord: 68..87
score: 31.4
coord: 309..335
score: 29.09
coord: 442..465
score: 31.06
coord: 355..373
score: 31.88
coord: 396..420
score: 35.49
IPR001128Cytochrome P450PRINTSPR00385P450coord: 356..367
score: 39.99
coord: 433..442
score: 60.95
coord: 442..453
score: 28.52
coord: 300..317
score: 40.76
IPR001128Cytochrome P450PFAMPF00067p450coord: 41..483
e-value: 5.9E-99
score: 331.9
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 28..499
e-value: 7.6E-112
score: 376.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 41..499
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 21..465
e-value: 3.4E-18
score: 63.6
NoneNo IPR availablePANTHERPTHR47956:SF23(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKEcoord: 15..497
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 15..497
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 435..444

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0011293.1Tan0011293.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen