Tan0011173 (gene) Snake gourd v1

BLAST of Tan0011173 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 1.2e-134
Identity = 255/540 (47.22%), Postives = 348/540 (64.44%), Query Frame = 0

Query: 10  LLILVSIFHFQLRVLPSH---TIPNQDASYMKFVHDANYLPKKEEYDYIIIGGGTAGCPL 69
           +L+++ +F   L+    H   T  N D SY++F +DA  L  +  YDY+I+GGGT+GCPL
Sbjct: 9   ILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPL 68

Query: 70  AATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVENIRGR 129
           AATLS K+ VL+LERGS+P  YP+VL     +     EDDGK P  RF+SEDG++N+RGR
Sbjct: 69  AATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGR 128

Query: 130 VLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAWQSAFRSA 189
           VLGG+SMINAG Y+R +   +  +G+DWDM+LV+K+Y+WVE T+V +P    WQS   +A
Sbjct: 129 VLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTA 188

Query: 190 LLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIHATVERII 249
            LEAGV P++GF L H  GT+I GS FD+KG RH A E LNK    NL+V +HA+VE+II
Sbjct: 189 FLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKII 248

Query: 250 IS---GASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLK 309
            S   G +A GV Y DSNG  H AF+  KGE+I+SAG IG+PQLLLLSG+GP+S+LSSL 
Sbjct: 249 FSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLN 308

Query: 310 LPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQA----------- 369
           +PVVL  P+VG+ + DNPR   NI+ P  +  + V V+G  ++N  +Q            
Sbjct: 309 IPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG--ISNDFYQCSFSSLPFTTPP 368

Query: 370 ----------VSSSSSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKC 429
                     + +S+ A    K +  LS GSL L SS++V+ SP V+FNYYS+P D++ C
Sbjct: 369 FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHC 428

Query: 430 VRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDD--------NSVGEFF- 489
           V G++K+GELL T  ++  K +DL G + F  LG   PK+ +DD         SV  ++ 
Sbjct: 429 VSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWH 488

Query: 490 ----------VDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQERSS 504
                     +D ++RV GI  LRVVDGSTF  +P ++P    +MLGRYVG+KILQERS+
Sbjct: 489 YHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERSA 546

BLAST of Tan0011173 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 2.9e-133
Identity = 252/543 (46.41%), Postives = 350/543 (64.46%), Query Frame = 0

Query: 5   LATFLLLILVSIFHFQLRVLPS-HTIPNQDASYMKFVHDANYLPKKEEYDYIIIGGGTAG 64
           ++  LL++ + + H Q   + S  T  + D SY+ F +DA  L  +  YDY+I+GGGT+G
Sbjct: 6   MSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSG 65

Query: 65  CPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVENI 124
           CPLAATLS K+ VL+LERGS+P  YP+VL     +     EDDGK P  RF+SEDG++N+
Sbjct: 66  CPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 125 RGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAWQSAF 184
           RGRVLGG+S+INAG Y+R +   +  +G+DWDM+LV+++Y+WVE T+V +P   +WQS  
Sbjct: 126 RGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVT 185

Query: 185 RSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIHATVE 244
           ++A LEAGV P++GF L H  GT+I GS FD+KG RH A E LNK    NL+V +HA+VE
Sbjct: 186 KTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVE 245

Query: 245 RIIIS---GASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 304
           +II S   G +A GV Y DSNG  H AF+  KGE+I+SAG IG+PQLLLLSG+GP+S+LS
Sbjct: 246 KIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLS 305

Query: 305 SLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQA-------- 364
           SL +PVVL  P+VG+ + DNPR   NI+ P  +  + V V+G  ++N  +Q         
Sbjct: 306 SLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG--ISNDFYQCSFSSLPFT 365

Query: 365 -------------VSSSSSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDV 424
                        + +S+ A    K +  LS GSL L SS++V+ SP V+FNYYS+  D+
Sbjct: 366 TPPFGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDL 425

Query: 425 AKCVRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDD--------NSVGE 484
           + CV G++K+GELL T  ++  K +DL G + F  LG   PK+ +DD         SV  
Sbjct: 426 SHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVAS 485

Query: 485 FF-----------VDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQE 504
           ++           +D ++RV GI  LRVVDGSTF  +P ++P    +MLGRYVG+KILQE
Sbjct: 486 YWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 545

BLAST of Tan0011173 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.1e-131
Identity = 249/542 (45.94%), Postives = 348/542 (64.21%), Query Frame = 0

Query: 5   LATFLLLILVSIFHFQLRVLPS-HTIPNQDASYMKFVHDANYLPKKEEYDYIIIGGGTAG 64
           ++  LL++ + + H Q   + S     + D SY+ FV+DA     +  YDYII+GGGTAG
Sbjct: 6   MSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAG 65

Query: 65  CPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVENI 124
           CPLAATLS+ +SVL+LERGS+P +YP++L     +     EDDGK P  RF+SEDG++N+
Sbjct: 66  CPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 125 RGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAWQSAF 184
           RGRVLGG+SMINAG Y R +  FF   GI+WDM+LV+++Y+WVE T+V  P    WQ+  
Sbjct: 126 RGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVI 185

Query: 185 RSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIHATVE 244
            +A LEAG++P+NGF + HL GT++ GS FD+ G RH + E LNK +P NL+VA+ A VE
Sbjct: 186 GTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVE 245

Query: 245 RIII----SGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHL 304
           +II     SG +A GV Y+DSNG  H AF+  +GE+ILSAG IGSPQLLLLSG+GP+S+L
Sbjct: 246 KIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYL 305

Query: 305 SSLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTL-------LNNIHFQA 364
           +SL + VV   P+VG+ + DNPR   NI+ P  + AS+V V+G         ++++ F  
Sbjct: 306 TSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSDFYQCSISSLPFDT 365

Query: 365 ------------VSSSSSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVA 424
                       + + + A I+ K    LS G++ LNSS+DV+  P V+FNYYS+  D++
Sbjct: 366 PPFSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLS 425

Query: 425 KCVRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDD--------NSVGEF 484
            CV G++KLGE+L T  +E  K +DL G   F  LG   P+N +DD         SV  +
Sbjct: 426 HCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVASY 485

Query: 485 F-----------VDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 504
           +           +D  +RV GI  LRVVDGSTF  +P ++P    +MLGRY+G++ILQER
Sbjct: 486 WHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQER 545

BLAST of Tan0011173 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 2.8e-128
Identity = 245/539 (45.45%), Postives = 344/539 (63.82%), Query Frame = 0

Query: 9   LLLILVSIFHFQLRVLPSHTIPN---QDASYMKFVHDANYLPKKEEYDYIIIGGGTAGCP 68
           +L++ + +F   L+     ++ N    D SY++FV+DAN    +  YDYII+GGGTAGCP
Sbjct: 9   ILVLALHLFVLHLQYSEVQSLANTSAHDFSYLEFVYDANDTELEGTYDYIIVGGGTAGCP 68

Query: 69  LAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVENIRG 128
           LAATLS+ +SVL+LERG++P +YP++L     +     EDDG+ P  RF+S DG++N+RG
Sbjct: 69  LAATLSANYSVLVLERGTLPTEYPNLLTSDGFIYNLQQEDDGQTPVERFVSGDGIDNVRG 128

Query: 129 RVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAWQSAFRS 188
           RVLGG+SMINAG Y R +  FF   GI+WDM+LV+K+Y WVE T+V +P    WQ+   +
Sbjct: 129 RVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNKTYDWVEDTIVFKPDFQFWQNLTGT 188

Query: 189 ALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIHATVERI 248
           A LE G++PDNGF L HL GT++ GS FD+ G RH + E LNK +P NL+VA+HA VE+I
Sbjct: 189 AFLEVGILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVHAAVEKI 248

Query: 249 II----SGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLSS 308
           I     SG +A GV Y+DSNG  H AF+   GE+ILSAG IGSPQLLLLSG+G +S+L+S
Sbjct: 249 IFSSDSSGVTAIGVIYTDSNGTTHQAFVRGDGEVILSAGPIGSPQLLLLSGVGLESYLTS 308

Query: 309 LKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTL-------LNNIHFQA-- 368
           L + VV   P+VG+ + DNPR   NI+ P  + AS+V V+G         ++++ F    
Sbjct: 309 LNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSDFYQCSISSLPFSTAP 368

Query: 369 ----------VSSSSSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKC 428
                     + +++ A I+ K    LS G++ L S++DV+ +P V FNYYS+  D+A C
Sbjct: 369 FGFFPNPTYPLPNTTFAHIVNKVPGPLSHGTVLLQSTSDVRVAPNVTFNYYSNTTDLAHC 428

Query: 429 VRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNS-------------- 488
           V G++K+GE L +  ++  K +DL G + F  LG   P+N +DD +              
Sbjct: 429 VSGMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIPLPENQTDDAAFETFCREAVASYWH 488

Query: 489 ------VGEFFVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 502
                 VGE  +D ++RV GI  LRVVDGSTF  +P ++P    +MLGRY+G KILQER
Sbjct: 489 YHGGCLVGE-VLDDDFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGTKILQER 546

BLAST of Tan0011173 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.7e-128
Identity = 242/519 (46.63%), Postives = 329/519 (63.39%), Query Frame = 0

Query: 31  NQDASYMKFVHDANYLPKKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSVPNKYPS 90
           N+   +M+F+ +A     ++ YDYII+GGGTAGCPLAATLS  F VLLLERG VP   P+
Sbjct: 33  NRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPN 92

Query: 91  VLKEQDLLNIFSTEDDGKNPFNRFISEDGVENIRGRVLGGSSMINAGFYSRGHREFFETA 150
           V+     L   +  ++  +P   FISE+GV N RGRVLGGSS INAGFYSR  ++FFE +
Sbjct: 93  VMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENS 152

Query: 151 GIDWDMELVDKSYQWVEQTVVSRPILNAWQSAFRSALLEAGVVPDNGFDLRHLVGTKIGG 210
           G+ WD+  V++SY+WVE+ +V RP L  WQ+A R ALLE GV P NGF L H VGTKIGG
Sbjct: 153 GLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGG 212

Query: 211 SIFDHKGNRHGAVEFLNKVEPKNLKVAIHATVERIII--------SGASANGVSYSDSNG 270
           S FD  G RH + + L      N++VA++ATVER+++        S  SA GV Y D  G
Sbjct: 213 STFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLG 272

Query: 271 KLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSDNPR 330
           + H A I  +GE+ILSAGA+GSPQLL LSGIGP+S+LS+  +PV L QPHVG+ + DNPR
Sbjct: 273 RFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPR 332

Query: 331 FGANIVLPMQLAASSVRVVGTLLNNIHFQAVSS---------------------SSSAII 390
            G +IV P+ +  S ++VVG   +    +A S+                          I
Sbjct: 333 NGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTI 392

Query: 391 LGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKCVRGLRKLGELLQTQTMEKI 450
           + K    +S+G LRL +STDV+ +P+VRFNY+S P D+ +CV G RK+GE+L+++ M+  
Sbjct: 393 MEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDF 452

Query: 451 KTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF-------------------FVDSNYRVIG 502
             ++  G + F+F+G   P + S+D  + +F                    VDS+ +VIG
Sbjct: 453 MIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIG 512

BLAST of Tan0011173 vs. NCBI nr
Match: XP_022153314.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 768.1 bits (1982), Expect = 4.8e-218
Identity = 396/542 (73.06%), Postives = 440/542 (81.18%), Query Frame = 0

Query: 1   MDHF-LATFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGG 60
           M H+ +A FLLL +VSIFHFQL+VL SHTIPNQD SYMKFV DA  LP+KEEYDYII+GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDG 120
           GTAGCPLA TLSSKFSVLLLERGS PNK+PSVL E+ LLN F+ EDD KNPF RFISEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAW 180
           VEN+RGRVLGGSSMINAGFYSRGHRE+FE AG++WDMELV+K+YQWVE+TVVSRP L+A 
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHREYFEKAGVEWDMELVEKAYQWVEETVVSRPSLSAL 180

Query: 181 QSAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIH 240
           Q  FRSALLE+GV+PDNGF LRHLVGT+IGGSIFD KGNRHGAVE LNK  P NLKVAI 
Sbjct: 181 QPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNRHGAVELLNKANPTNLKVAIR 240

Query: 241 ATVERIIISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
           ATVERII SG SA+GVSYSDS GKLHTAFI  KGEIILSAGAIGSPQLLLLSGIGPKS L
Sbjct: 241 ATVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQL 300

Query: 301 SSLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSSS--- 360
           SSLKLPVVLHQP+VGE MSDNPRFG +IVLP     +S +VVGTL NNIH Q V+S    
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPFVPTSAKVVGTLENNIHLQTVASPLPF 360

Query: 361 ------------------SSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
                             S  I  GKFSEVLS GSLRLNSSTDVKKSPIVRFNYYSHPDD
Sbjct: 361 SIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420

Query: 421 VAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF------ 480
           +A+CV G+RK+G+L++T TMEKIKT+D EGKK FQF+GP  P+N+SDD++VGEF      
Sbjct: 421 LARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLT 480

Query: 481 -------------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQ 502
                         VD +YRVIGI +LRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQ 540

BLAST of Tan0011173 vs. NCBI nr
Match: XP_022153315.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 762.3 bits (1967), Expect = 2.6e-216
Identity = 393/542 (72.51%), Postives = 438/542 (80.81%), Query Frame = 0

Query: 1   MDHF-LATFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGG 60
           M H+ +A FLLL +VSIFHFQL+VL SHTIPNQD SYMKFV DA  LP+KEEYDYII+GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDG 120
           GTAGCPLA TLSSKFSVLLLERGS PNK+PSVL E+ LLN F+ EDD KNPF RFISEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAW 180
           VEN+RGRVLGGSSMINAGFYSRGH+EFFE AG++WDMELV+K+YQWVE+TVVSRP L+A 
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHQEFFEKAGVEWDMELVEKAYQWVEETVVSRPSLSAL 180

Query: 181 QSAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIH 240
           Q  FRSALLE+GV+PDNGF LRHLVGT+IGGSIFD KGN HGAVE LNK  P N+KVAI 
Sbjct: 181 QPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNPHGAVELLNKANPTNIKVAIR 240

Query: 241 ATVERIIISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
            TVERII SG SA+GVSYSDS GKLHTAFI  KGEIILSAGAIGSPQLLLLSGIGPKS L
Sbjct: 241 TTVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQL 300

Query: 301 SSLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSSS--- 360
           SSLKLPVVLHQP+VGE MSDNPRFG +IVLP     +S +VVGTL NNIH Q V+S    
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPXVPTSAKVVGTLENNIHLQTVASPLPF 360

Query: 361 ------------------SSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
                             S  I  GKFSEVLS GSLRLNSSTDVKKSPIVRFNYYSHPDD
Sbjct: 361 SIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420

Query: 421 VAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF------ 480
           +A+CV G+RK+G+L++T TMEKIKT+D EGKK FQF+GP  P+N+SDD++VGEF      
Sbjct: 421 LARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLT 480

Query: 481 -------------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQ 502
                         VD +YRVIGI +LRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQ 540

BLAST of Tan0011173 vs. NCBI nr
Match: XP_038889314.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 757.7 bits (1955), Expect = 6.5e-215
Identity = 391/543 (72.01%), Postives = 441/543 (81.22%), Query Frame = 0

Query: 1   MDHFLATFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGGG 60
           M+H  AT L+L L+ I   QL VL  + IP +D  YMKFVH A+ LP KEEYDYIIIGGG
Sbjct: 1   MEHSKATILILSLM-ISISQLGVLTYNAIPIKDVRYMKFVHKASDLPAKEEYDYIIIGGG 60

Query: 61  TAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGV 120
           TAGCPLAATLSSKFSVL+LERGS PNKYPSVL EQ LLN+F+  DDGKNPF RFISEDGV
Sbjct: 61  TAGCPLAATLSSKFSVLVLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFISEDGV 120

Query: 121 ENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAWQ 180
           ENIR RVLGG SM+NAGFYSRGH++FFETAG+DWDMELV+K+YQWVE+TV SRPILNAWQ
Sbjct: 121 ENIRARVLGGGSMVNAGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVASRPILNAWQ 180

Query: 181 SAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIHA 240
           SAFRSALLEAGVVPDNGFDLRHLVGTK GGSIFD KGNRHGAVE LNK EP N+KVAI A
Sbjct: 181 SAFRSALLEAGVVPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPTNIKVAIEA 240

Query: 241 TVERIIISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 300
           TV++I+ SG SA GV YSDS GKLH AFI KKGEII+SAGAIGSPQLLLLSG+GPKSHLS
Sbjct: 241 TVQKILFSGLSARGVLYSDSKGKLHRAFIRKKGEIIVSAGAIGSPQLLLLSGVGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSSSSS-- 360
           SLKLPVVLHQP+VGE MSDNPRFG NI+LP QL  SSV+VVG L +NI+ Q+ +S S   
Sbjct: 301 SLKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFF 360

Query: 361 -------------------AIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDV 420
                              A+ +GKFSEV S GSLRLNSST+VKKSPIVRFNYYSHPDD+
Sbjct: 361 APPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDL 420

Query: 421 AKCVRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF------- 480
           A+CV+G+RK+G+LL+TQTMEKIKT DLEG K FQFLG   P+N+S+D+SVGE+       
Sbjct: 421 ARCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVGEYCQKTVTT 480

Query: 481 ------------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQE 504
                        VD NY+VIGIK+LRVVDGSTFS+SPGTNPMATLMMLGRYVG+K+LQ+
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQ 540

BLAST of Tan0011173 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 756.9 bits (1953), Expect = 1.1e-214
Identity = 386/544 (70.96%), Postives = 441/544 (81.07%), Query Frame = 0

Query: 1   MDHFLATFLLL-ILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGG 60
           M+H  +T L+L +++SIFH  L +L SH IPNQD  YMKFVH A  LP KEEYDYIIIGG
Sbjct: 1   MEHSRSTILILSLMISIFH--LGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGG 60

Query: 61  GTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDG 120
           GTAGCPL++TLSSKFSVLLLERGS PNKYPSVL EQ LLN F+ EDDGKNPF  FISEDG
Sbjct: 61  GTAGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDG 120

Query: 121 VENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAW 180
           VENIRGRVLGG SM+N GFYSRGH++FFETAG+DWDMELV+K+YQWVE+TVVS+PILNAW
Sbjct: 121 VENIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPILNAW 180

Query: 181 QSAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIH 240
           QSAFRSA L+AGVVPDNGFDLRH VGTKIGGSIFD+KGNRHGAVE LNK  P N+KV I 
Sbjct: 181 QSAFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIE 240

Query: 241 ATVERIIISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
           ATV+RI+ SG SA GV YSDS GKLH AFI K+GEII+SAGA+GSPQLLLLSG+GPKSHL
Sbjct: 241 ATVQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHL 300

Query: 301 SSLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSSSSS- 360
           SSLKLPVVLHQP+VGE MSDNPRFG NI+LP QL  SSV+VVG L +NI+ Q+ +S S  
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPF 360

Query: 361 --------------------AIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
                               A+ +GKFSEV S GSLRLNSST+VKKSPIVRFNYYSHPDD
Sbjct: 361 FAPPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDD 420

Query: 421 VAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF------ 480
           +A+CV+G+RK+G+LL+TQTMEKIKT DLEG K FQFLG   P+N+S+D+SV E+      
Sbjct: 421 LARCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVT 480

Query: 481 -------------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQ 504
                         VD NY+VIGI++LRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFCDSPGTNPMATLMMLGRYVGLKVLQ 540

BLAST of Tan0011173 vs. NCBI nr
Match: XP_022153313.1 ((R)-mandelonitrile lyase 1-like [Momordica charantia])

HSP 1 Score: 753.8 bits (1945), Expect = 9.3e-214
Identity = 380/535 (71.03%), Postives = 435/535 (81.31%), Query Frame = 0

Query: 7   TFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGGGTAGCPL 66
           TFLLL ++SIFHFQL VL SHTI NQD SYMKFVHDA  LP+  EYDYII+GGG AGCPL
Sbjct: 8   TFLLLFIISIFHFQLGVLSSHTISNQDVSYMKFVHDAKDLPETGEYDYIIVGGGAAGCPL 67

Query: 67  AATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVENIRGR 126
           A TLSSKFS+L+LERGS PNK+PSVL EQ     F+ EDDGKNPF RF+SEDGVEN+RGR
Sbjct: 68  ATTLSSKFSILILERGSEPNKFPSVLTEQGRSKAFTAEDDGKNPFQRFVSEDGVENLRGR 127

Query: 127 VLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAWQSAFRSA 186
           VLGG +MIN GFYSRGHREFF+TAG++WDMELV+K+YQWVE+TVVSRP L+AWQSAFRSA
Sbjct: 128 VLGGGTMINGGFYSRGHREFFDTAGVEWDMELVEKAYQWVEETVVSRPNLSAWQSAFRSA 187

Query: 187 LLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIHATVERII 246
           LLE+GV+PDNGFDLRHLVGT+IGGS FD KGNRHGAVE LNK  P NLKV I ATVERII
Sbjct: 188 LLESGVLPDNGFDLRHLVGTRIGGSTFDGKGNRHGAVELLNKANPANLKVVIRATVERII 247

Query: 247 ISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPV 306
            SG SA+GVSYSDS GKLHTAFI KKGEI LSAGAIGSPQLLLLSG+GPKS LSSLKLPV
Sbjct: 248 FSGLSASGVSYSDSEGKLHTAFIRKKGEIFLSAGAIGSPQLLLLSGVGPKSDLSSLKLPV 307

Query: 307 VLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSSS---------- 366
           VLHQPHVG+ MSDNPRF  NIVLP  + +++ +VVGTL NNIHFQ+ + S          
Sbjct: 308 VLHQPHVGQFMSDNPRFVTNIVLPFPIVSTTGKVVGTLKNNIHFQSFAGSSLFSVPPSFS 367

Query: 367 -----------SSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKCVRG 426
                      S A ++GKFS+V S+GSLRLNSSTDVKKSPIVRFNYYSHPDD+A+CV G
Sbjct: 368 LLPPRSNSVNMSLATVVGKFSKVDSMGSLRLNSSTDVKKSPIVRFNYYSHPDDLARCVVG 427

Query: 427 LRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF------------- 486
           +RK+G+L++T TMEKIKT++ EGKK FQFLGP  P+N+SD +SVGEF             
Sbjct: 428 VRKIGDLIKTPTMEKIKTRNFEGKKVFQFLGPPLPENLSDYSSVGEFCHKTLTTYWHYHG 487

Query: 487 ------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 502
                  VD +YRVIGI +LRVVDGSTF++SPGTNPMATLMMLGRYVG+K++Q+R
Sbjct: 488 GCLVGKVVDGDYRVIGIGNLRVVDGSTFTESPGTNPMATLMMLGRYVGLKVMQQR 542

BLAST of Tan0011173 vs. ExPASy TrEMBL
Match: A0A6J1DIQ0 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020835 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.3e-218
Identity = 396/542 (73.06%), Postives = 440/542 (81.18%), Query Frame = 0

Query: 1   MDHF-LATFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGG 60
           M H+ +A FLLL +VSIFHFQL+VL SHTIPNQD SYMKFV DA  LP+KEEYDYII+GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDG 120
           GTAGCPLA TLSSKFSVLLLERGS PNK+PSVL E+ LLN F+ EDD KNPF RFISEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAW 180
           VEN+RGRVLGGSSMINAGFYSRGHRE+FE AG++WDMELV+K+YQWVE+TVVSRP L+A 
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHREYFEKAGVEWDMELVEKAYQWVEETVVSRPSLSAL 180

Query: 181 QSAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIH 240
           Q  FRSALLE+GV+PDNGF LRHLVGT+IGGSIFD KGNRHGAVE LNK  P NLKVAI 
Sbjct: 181 QPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNRHGAVELLNKANPTNLKVAIR 240

Query: 241 ATVERIIISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
           ATVERII SG SA+GVSYSDS GKLHTAFI  KGEIILSAGAIGSPQLLLLSGIGPKS L
Sbjct: 241 ATVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQL 300

Query: 301 SSLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSSS--- 360
           SSLKLPVVLHQP+VGE MSDNPRFG +IVLP     +S +VVGTL NNIH Q V+S    
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPFVPTSAKVVGTLENNIHLQTVASPLPF 360

Query: 361 ------------------SSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
                             S  I  GKFSEVLS GSLRLNSSTDVKKSPIVRFNYYSHPDD
Sbjct: 361 SIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420

Query: 421 VAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF------ 480
           +A+CV G+RK+G+L++T TMEKIKT+D EGKK FQF+GP  P+N+SDD++VGEF      
Sbjct: 421 LARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLT 480

Query: 481 -------------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQ 502
                         VD +YRVIGI +LRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQ 540

BLAST of Tan0011173 vs. ExPASy TrEMBL
Match: A0A6J1DGG8 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020836 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.3e-216
Identity = 393/542 (72.51%), Postives = 438/542 (80.81%), Query Frame = 0

Query: 1   MDHF-LATFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGG 60
           M H+ +A FLLL +VSIFHFQL+VL SHTIPNQD SYMKFV DA  LP+KEEYDYII+GG
Sbjct: 1   MKHYSMAIFLLLFIVSIFHFQLQVLSSHTIPNQDVSYMKFVEDAKDLPEKEEYDYIIVGG 60

Query: 61  GTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDG 120
           GTAGCPLA TLSSKFSVLLLERGS PNK+PSVL E+ LLN F+ EDD KNPF RFISEDG
Sbjct: 61  GTAGCPLATTLSSKFSVLLLERGSEPNKFPSVLVEEGLLNAFAAEDDDKNPFQRFISEDG 120

Query: 121 VENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAW 180
           VEN+RGRVLGGSSMINAGFYSRGH+EFFE AG++WDMELV+K+YQWVE+TVVSRP L+A 
Sbjct: 121 VENLRGRVLGGSSMINAGFYSRGHQEFFEKAGVEWDMELVEKAYQWVEETVVSRPSLSAL 180

Query: 181 QSAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIH 240
           Q  FRSALLE+GV+PDNGF LRHLVGT+IGGSIFD KGN HGAVE LNK  P N+KVAI 
Sbjct: 181 QPTFRSALLESGVLPDNGFGLRHLVGTRIGGSIFDGKGNPHGAVELLNKANPTNIKVAIR 240

Query: 241 ATVERIIISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHL 300
            TVERII SG SA+GVSYSDS GKLHTAFI  KGEIILSAGAIGSPQLLLLSGIGPKS L
Sbjct: 241 TTVERIIFSGLSASGVSYSDSEGKLHTAFIRNKGEIILSAGAIGSPQLLLLSGIGPKSQL 300

Query: 301 SSLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSSS--- 360
           SSLKLPVVLHQP+VGE MSDNPRFG +IVLP     +S +VVGTL NNIH Q V+S    
Sbjct: 301 SSLKLPVVLHQPYVGEFMSDNPRFGPSIVLPFPXVPTSAKVVGTLENNIHLQTVASPLPF 360

Query: 361 ------------------SSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDD 420
                             S  I  GKFSEVLS GSLRLNSSTDVKKSPIVRFNYYSHPDD
Sbjct: 361 SIPPSFSLLPPQSNSVNLSLVIFTGKFSEVLSEGSLRLNSSTDVKKSPIVRFNYYSHPDD 420

Query: 421 VAKCVRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF------ 480
           +A+CV G+RK+G+L++T TMEKIKT+D EGKK FQF+GP  P+N+SDD++VGEF      
Sbjct: 421 LARCVVGVRKMGDLIKTPTMEKIKTRDFEGKKVFQFIGPSLPENLSDDSTVGEFCHKSLT 480

Query: 481 -------------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQ 502
                         VD +YRVIGI +LRVVDGSTF DSPGTNPMATLMMLGRYVG+K+LQ
Sbjct: 481 TYWHYHGGCLVGKVVDGDYRVIGIGNLRVVDGSTFIDSPGTNPMATLMMLGRYVGLKVLQ 540

BLAST of Tan0011173 vs. ExPASy TrEMBL
Match: A0A6J1DH55 ((R)-mandelonitrile lyase 1-like OS=Momordica charantia OX=3673 GN=LOC111020834 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.5e-214
Identity = 380/535 (71.03%), Postives = 435/535 (81.31%), Query Frame = 0

Query: 7   TFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGGGTAGCPL 66
           TFLLL ++SIFHFQL VL SHTI NQD SYMKFVHDA  LP+  EYDYII+GGG AGCPL
Sbjct: 8   TFLLLFIISIFHFQLGVLSSHTISNQDVSYMKFVHDAKDLPETGEYDYIIVGGGAAGCPL 67

Query: 67  AATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVENIRGR 126
           A TLSSKFS+L+LERGS PNK+PSVL EQ     F+ EDDGKNPF RF+SEDGVEN+RGR
Sbjct: 68  ATTLSSKFSILILERGSEPNKFPSVLTEQGRSKAFTAEDDGKNPFQRFVSEDGVENLRGR 127

Query: 127 VLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAWQSAFRSA 186
           VLGG +MIN GFYSRGHREFF+TAG++WDMELV+K+YQWVE+TVVSRP L+AWQSAFRSA
Sbjct: 128 VLGGGTMINGGFYSRGHREFFDTAGVEWDMELVEKAYQWVEETVVSRPNLSAWQSAFRSA 187

Query: 187 LLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIHATVERII 246
           LLE+GV+PDNGFDLRHLVGT+IGGS FD KGNRHGAVE LNK  P NLKV I ATVERII
Sbjct: 188 LLESGVLPDNGFDLRHLVGTRIGGSTFDGKGNRHGAVELLNKANPANLKVVIRATVERII 247

Query: 247 ISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPV 306
            SG SA+GVSYSDS GKLHTAFI KKGEI LSAGAIGSPQLLLLSG+GPKS LSSLKLPV
Sbjct: 248 FSGLSASGVSYSDSEGKLHTAFIRKKGEIFLSAGAIGSPQLLLLSGVGPKSDLSSLKLPV 307

Query: 307 VLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSSS---------- 366
           VLHQPHVG+ MSDNPRF  NIVLP  + +++ +VVGTL NNIHFQ+ + S          
Sbjct: 308 VLHQPHVGQFMSDNPRFVTNIVLPFPIVSTTGKVVGTLKNNIHFQSFAGSSLFSVPPSFS 367

Query: 367 -----------SSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKCVRG 426
                      S A ++GKFS+V S+GSLRLNSSTDVKKSPIVRFNYYSHPDD+A+CV G
Sbjct: 368 LLPPRSNSVNMSLATVVGKFSKVDSMGSLRLNSSTDVKKSPIVRFNYYSHPDDLARCVVG 427

Query: 427 LRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF------------- 486
           +RK+G+L++T TMEKIKT++ EGKK FQFLGP  P+N+SD +SVGEF             
Sbjct: 428 VRKIGDLIKTPTMEKIKTRNFEGKKVFQFLGPPLPENLSDYSSVGEFCHKTLTTYWHYHG 487

Query: 487 ------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQER 502
                  VD +YRVIGI +LRVVDGSTF++SPGTNPMATLMMLGRYVG+K++Q+R
Sbjct: 488 GCLVGKVVDGDYRVIGIGNLRVVDGSTFTESPGTNPMATLMMLGRYVGLKVMQQR 542

BLAST of Tan0011173 vs. ExPASy TrEMBL
Match: A0A6J1HRM5 ((R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465498 PE=3 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 3.2e-212
Identity = 388/539 (71.99%), Postives = 433/539 (80.33%), Query Frame = 0

Query: 5   LATFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGGGTAGC 64
           +AT L LIL+SIF   LRVL SHTIPNQD SYMK VHDA  +P+ EEYDYIIIGGGTAGC
Sbjct: 1   MATILPLILISIFF--LRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGC 60

Query: 65  PLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGVENIR 124
           PLAATLSSKFSVLLLERGS PNKYPSVL EQ LLN+F  EDDG+NPF RF+SE+GVE IR
Sbjct: 61  PLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIR 120

Query: 125 GRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAWQSAFR 184
           GRVLGG SM+NAGFYSRGHREFFE+AG+DWDMELV+K Y+WVE+TVVSRP L+AWQ AFR
Sbjct: 121 GRVLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFR 180

Query: 185 SALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIHATVER 244
            ALLE GV PDNGFDLRHLVGTK GGSIFD  GNRHGAVE LN VEP NLKVA+ ATV++
Sbjct: 181 RALLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQK 240

Query: 245 IIISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKL 304
           II S  SA GVSYSDS GK HTAFI KKGEIILSAGAIGSPQLLLLSG+GPKSHLSSLKL
Sbjct: 241 IIFSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKL 300

Query: 305 PVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSS--------- 364
           PVVLHQPHVGE MSDNPRF A +VLP  L ASS +VVG L  NIH Q  +S         
Sbjct: 301 PVVLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPT 360

Query: 365 ------------SSSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKCV 424
                        S A+++GKFS+VLS GSL LNSSTDVK +PIVRFNYY++P+D+A+CV
Sbjct: 361 FGLLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCV 420

Query: 425 RGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF----------- 484
           RGLRK+G+ L+T+TME+IKTQDLEG K FQFLGP  P+N+SD++SV E+           
Sbjct: 421 RGLRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHY 480

Query: 485 --------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQERSS 504
                    VD NYRVIGIK+LRVVDGSTFS SPGTNPMATLMMLGRYVG+K+LQERSS
Sbjct: 481 HGGCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 537

BLAST of Tan0011173 vs. ExPASy TrEMBL
Match: A0A6J1EP46 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436113 PE=3 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.7e-211
Identity = 379/543 (69.80%), Postives = 436/543 (80.29%), Query Frame = 0

Query: 1   MDHFLATFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGGG 60
           M+  +AT L LIL+S F F    + S+TIP+QD SYMKFVH+A  LP+K+EYDYIIIGGG
Sbjct: 1   MERSIATILFLILISTFQF--GAISSNTIPDQDVSYMKFVHNAGDLPEKQEYDYIIIGGG 60

Query: 61  TAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFSTEDDGKNPFNRFISEDGV 120
            AGCPLAATLSSKF VLLLERGS PNKYPSVL EQ LLN F  +DDG+NPF RF SEDGV
Sbjct: 61  AAGCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGV 120

Query: 121 ENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNAWQ 180
           ENIRGR+LGGS+M+NAGFYSRGHR+FFETAG++WDME+V+ +YQWVE+TVVS+PILNAWQ
Sbjct: 121 ENIRGRILGGSTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQ 180

Query: 181 SAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAIHA 240
           SAF+SALLEAGV PDNGF+L HL+GTK GGSIFD KGNRHGAVE LNK EP NLKVA++A
Sbjct: 181 SAFKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPMNLKVAVNA 240

Query: 241 TVERIIISGASANGVSYSDSNGKLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLS 300
           TV +I+ +G SANGVSYSDS GK+HTAFI KKGEI LSAGAIGSP LLL SG+GPKSHLS
Sbjct: 241 TVRQILFNGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGTLLNNIHFQAVSS----- 360
           SLKLPVV HQP+VGE MSDNPRFGA IVLP QLA+SSV+VVGTL +N+H QA +S     
Sbjct: 301 SLKLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFL 360

Query: 361 ----------------SSSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDV 420
                            S  I +GKFSEV S G LRLNS+TD K + IVRFNYYSHPDD+
Sbjct: 361 APPTFSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDL 420

Query: 421 AKCVRGLRKLGELLQTQTMEKIKTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF------- 480
           A+CV G+RK+G+LL+TQTMEKIKTQDLEG K FQF+G   P+N+ DD+SV E+       
Sbjct: 421 ARCVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTT 480

Query: 481 ------------FVDSNYRVIGIKHLRVVDGSTFSDSPGTNPMATLMMLGRYVGVKILQE 504
                        VD +YRVIG+K+LRVVDGSTFSDSPGTNPMATLMMLGRYVG+K+LQE
Sbjct: 481 YWHYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQE 540

BLAST of Tan0011173 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 459.9 bits (1182), Expect = 2.6e-129
Identity = 242/519 (46.63%), Postives = 329/519 (63.39%), Query Frame = 0

Query: 31  NQDASYMKFVHDANYLPKKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSVPNKYPS 90
           N+   +M+F+ +A     ++ YDYII+GGGTAGCPLAATLS  F VLLLERG VP   P+
Sbjct: 33  NRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPN 92

Query: 91  VLKEQDLLNIFSTEDDGKNPFNRFISEDGVENIRGRVLGGSSMINAGFYSRGHREFFETA 150
           V+     L   +  ++  +P   FISE+GV N RGRVLGGSS INAGFYSR  ++FFE +
Sbjct: 93  VMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENS 152

Query: 151 GIDWDMELVDKSYQWVEQTVVSRPILNAWQSAFRSALLEAGVVPDNGFDLRHLVGTKIGG 210
           G+ WD+  V++SY+WVE+ +V RP L  WQ+A R ALLE GV P NGF L H VGTKIGG
Sbjct: 153 GLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGG 212

Query: 211 SIFDHKGNRHGAVEFLNKVEPKNLKVAIHATVERIII--------SGASANGVSYSDSNG 270
           S FD  G RH + + L      N++VA++ATVER+++        S  SA GV Y D  G
Sbjct: 213 STFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLG 272

Query: 271 KLHTAFISKKGEIILSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSDNPR 330
           + H A I  +GE+ILSAGA+GSPQLL LSGIGP+S+LS+  +PV L QPHVG+ + DNPR
Sbjct: 273 RFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPR 332

Query: 331 FGANIVLPMQLAASSVRVVGTLLNNIHFQAVSS---------------------SSSAII 390
            G +IV P+ +  S ++VVG   +    +A S+                          I
Sbjct: 333 NGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTI 392

Query: 391 LGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKCVRGLRKLGELLQTQTMEKI 450
           + K    +S+G LRL +STDV+ +P+VRFNY+S P D+ +CV G RK+GE+L+++ M+  
Sbjct: 393 MEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDF 452

Query: 451 KTQDLEGKKAFQFLGPMFPKNMSDDNSVGEF-------------------FVDSNYRVIG 502
             ++  G + F+F+G   P + S+D  + +F                    VDS+ +VIG
Sbjct: 453 MIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIG 512

BLAST of Tan0011173 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 363.2 bits (931), Expect = 3.3e-100
Identity = 230/581 (39.59%), Postives = 304/581 (52.32%), Query Frame = 0

Query: 1   MDHFLATFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGGG 60
           MD F  ++ L + +S+F      L S    +  A    F+ DA   P    YDYIIIGGG
Sbjct: 2   MDRFW-SWRLFVALSLF------LHSPICSSDKAPNYSFMRDATGSPTTSYYDYIIIGGG 61

Query: 61  TAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFS---TEDDGKNPFNRFISE 120
           TAGCPLAATLS   SVLLLERG  P   P++ +    L+ F    ++    +P  RF+SE
Sbjct: 62  TAGCPLAATLSQNASVLLLERGDSPYNNPNITR----LSAFGAALSDLSESSPSQRFVSE 121

Query: 121 DGVENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILN 180
           DGV N R RVLGG S +NAGFY+R   ++    G  WD  L ++SYQWVE  V  +P + 
Sbjct: 122 DGVINARARVLGGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMG 181

Query: 181 AWQSAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVA 240
            WQ+A R  LLEAG+VP+NGF   H+ GTK GG+IFD  GNRH A + L   +PK + V 
Sbjct: 182 RWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVL 241

Query: 241 IHATVERIII-----SGASANGVSYSDSNGKLHTAFISK--KGEIILSAGAIGSPQLLLL 300
           +HATV RI+      +   ANGV Y D  G+ H A++ +    EIILSAG +GSPQLL+L
Sbjct: 242 LHATVHRILFRTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLML 301

Query: 301 SGIGPKSHLSSLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVG-------- 360
           SG+GP + L +  + VV+ QPHVG+ M DNP     +  P+ +  S + VVG        
Sbjct: 302 SGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYV 361

Query: 361 --------------------------------TLLNNIHFQAVSSS---SSAIILGKFSE 420
                                           TLL +     +SS+       +L K   
Sbjct: 362 EAAGGENFGGGGGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMG 421

Query: 421 VLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQD-- 480
            LS G L L  + + K +PIV FNY+ HPDD+ +CVRG++ +  ++Q++   + K  D  
Sbjct: 422 PLSTGHLEL-KTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVS 481

Query: 481 ------LEGKKAFQFLGPMFPKNMSDDNSVGEF-------------------FVDSNYRV 502
                 L          P      S   S  EF                    VD +Y+V
Sbjct: 482 FEYLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKV 541

BLAST of Tan0011173 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 344.0 bits (881), Expect = 2.1e-94
Identity = 210/531 (39.55%), Postives = 282/531 (53.11%), Query Frame = 0

Query: 52  YDYIIIGGGTAGCPLAATLSSKFSVLLLERGSVP--NKYPSVLKEQDLLNIFSTEDDGKN 111
           YDYI+IGGGTAGCPLAATLS  FSVL+LERG VP  N   S L+     +I   +    +
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADISASS 122

Query: 112 PFNRFISEDGVENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQT 171
               F+S DGV N R RVLGG S INAGFYSR    F + AG  WD +LV +SY WVE+ 
Sbjct: 123 ASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVERE 182

Query: 172 VVSRPILNAWQSAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKV 231
           +V +P L  WQ A R +LLE GV P NGF   H+ GTKIGG+IFD  G RH A E L   
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 232 EPKNLKVAIHATVERIIISGAS----ANGVSYSDSNGKLHTAFIS--KKGEIILSAGAIG 291
            P+ L+V I+ATV++I+   +       GV + D  G  H A +S  K  E+ILS+GAIG
Sbjct: 243 NPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIG 302

Query: 292 SPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGT 351
           SPQ+L+LSGIGPK  L  LK+PVVL   HVG+ M+DNP     +     +  S ++ VG 
Sbjct: 303 SPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGI 362

Query: 352 LLNNIH-------------------------------------------------FQAVS 411
               ++                                                 +Q   
Sbjct: 363 TKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHE 422

Query: 412 SSSSAIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKCVRGLRKLGELLQ 471
           + + + IL K +  +S G L L  +T+V  +P V FNY+ HP D+ +CV  +R + +++ 
Sbjct: 423 AFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVT 482

Query: 472 TQTMEKIKTQDLEGKKAFQFLG-----PMFPKNMSDDNSVGEFFVDS------------- 502
           +         D +       L       + PK ++D  S+ +F  D+             
Sbjct: 483 SNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLV 542

BLAST of Tan0011173 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 342.4 bits (877), Expect = 6.0e-94
Identity = 214/553 (38.70%), Postives = 294/553 (53.16%), Query Frame = 0

Query: 34  ASYMKFVHDANYLPKKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSVPNKYPSVLK 93
           A Y  F+ DA   P    +DYIIIGGGT+GC LAATLS   SVL+LERG  P   P+   
Sbjct: 28  AGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATD 87

Query: 94  EQDLLNIFSTEDDGKNPFNRFISEDGVENIRGRVLGGSSMINAGFYSRGHREFFETAGID 153
            ++     S     K+    FISEDGV N R RVLGG S++NAGFY+R   E+ +    +
Sbjct: 88  IENFATTLSNTSP-KSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKET--E 147

Query: 154 WDMELVDKSYQWVEQTVVSRPILNAWQSAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIF 213
           W  + V+ +Y+WVE+ V  +P +  WQ+AF+  LLEAG  P NGF   H+ GTKIGG+IF
Sbjct: 148 WKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIF 207

Query: 214 DHKGNRHGAVEFLNKVEPKNLKVAIHATVERIIIS-----GASANGVSYSDSNGKLHTAF 273
           D  G+RH A + L    P N+ V +HA+V +I+ +        A GV + D+NG LH A 
Sbjct: 208 DRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAE 267

Query: 274 ISKK--GEIILSAGAIGSPQLLLLSGIGPKSHLSSLKL-PVVLHQPHVGESMSDNPRFGA 333
           + K    E+ILSAGAIGSPQLL+LSGIGP +HL++  + P+VL  P VG+ M DNP    
Sbjct: 268 LEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAI 327

Query: 334 NIVLPMQLAASSVRVVG-----------------------------TLLNNIHFQAVSSS 393
            I  P  +  S ++VVG                               LN +     +S+
Sbjct: 328 FIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTST 387

Query: 394 SS-------------------------AIILGKFSEVLSVGSLRLNSSTDVKKSPIVRFN 453
           +S                          +IL K +  +S G L L  +T+   +P VRFN
Sbjct: 388 TSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR-NTNPDDNPSVRFN 447

Query: 454 YYSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQD--LEGKKAFQFLGP--MFPKNMSDDN 502
           YY  P+D+  CV G+  + +++ ++   K K  D  + G        P  + P++++   
Sbjct: 448 YYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMF 507

BLAST of Tan0011173 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 340.1 bits (871), Expect = 3.0e-93
Identity = 223/580 (38.45%), Postives = 312/580 (53.79%), Query Frame = 0

Query: 1   MDHFLATFLLLILVSIFHFQLRVLPSHTIPNQDASYMKFVHDANYLPKKEEYDYIIIGGG 60
           M+  +  F   ILV++F F        +  + +A   +F+ DA   PK   +DYIIIGGG
Sbjct: 1   MNFHIFRFFQFILVAVFIFH------DSCYSDEAGNYRFMKDATLAPKLSHFDYIIIGGG 60

Query: 61  TAGCPLAATLSSKFSVLLLERGSVPNKYPSVLKEQDLLNIFST-EDDGKNPFNR-FISED 120
           TAGC LAATLS   +VL+LERG  P   P+     D+ N  +T  +   N +++ FISED
Sbjct: 61  TAGCALAATLSQNATVLVLERGGSPYDDPAA---TDIGNFANTLLNITPNSWSQLFISED 120

Query: 121 GVENIRGRVLGGSSMINAGFYSRGHREFFETAGIDWDMELVDKSYQWVEQTVVSRPILNA 180
           GV N R RVLGG ++INAGFYSR   +F   AG  W+ + V+ +Y+WVE+ VV  P +N 
Sbjct: 121 GVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNK 180

Query: 181 WQSAFRSALLEAGVVPDNGFDLRHLVGTKIGGSIFDHKGNRHGAVEFLNKVEPKNLKVAI 240
           WQSAFR  LLEAGV P NGF   H+VGTK GG+IFD  G+RH A   L    P  + V +
Sbjct: 181 WQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYL 240

Query: 241 HATVERIIIS-----GASANGVSYSDSNGKLHTAFI----SKKGEIILSAGAIGSPQLLL 300
           HA+V +I+ +        A GV + D+NG  + A +    S   E+ILSAGAI SPQLL+
Sbjct: 241 HASVHKILFTIKGNQRPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLM 300

Query: 301 LSGIGPKSHLSSLKL-PVVLHQPHVGESMSDNPRFGANIVLPMQLAASSVRVVGT----- 360
           LSG+GP +HL++ ++ PV++ QP VG+ M DNP     I  P  +  S V+ VG      
Sbjct: 301 LSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGS 360

Query: 361 ---------------------LLN-----NIHFQAVS------------SSSSAIILGKF 420
                                +LN      +  Q++S            ++ + +I+ K 
Sbjct: 361 YIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKV 420

Query: 421 SEVLSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVAKCVRGLRKLGELLQTQTMEKIKTQD 480
           +  LS G L L  +T+   +P V FNY+  P+D+ KCV GL  + +++ ++   K K   
Sbjct: 421 NGPLSRGHLELR-NTNPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYK-YP 480

Query: 481 LEGKKAFQFLGPMFPKNMSDDNSVGEF------------------------FVDSNYRVI 502
           L   +    L    P N+   +    F                         VD+NY+V+
Sbjct: 481 LASARGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVL 540