Tan0011096 (gene) Snake gourd v1

Overview
NameTan0011096
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionAuxin-responsive GH3 family protein
LocationLG01: 17182039 .. 17184363 (+)
RNA-Seq ExpressionTan0011096
SyntenyTan0011096
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGAAGCACCAAACACAATCTCTGTGGAAAAGAACAAGGCGAGTCTGGAATACATCGAAGAAATAACGAGGAACGCCGGAAATGTCCAGCGCCAAGTTCTGTCTCAAATCCTGTCTCGAAATGCCTCCTCCGAGTACTTAACCCTCCATGGGCGTCCTAATTCCCCTGAGACCTTCAAATCATCCATTCCTTTAATCTCTTACAATGAAATAGAACCCTTCATCACCCGCATCGCCAATGGCGATACTTCCCCTATTCTTTGCTCTTCCCCGATCTCCGAATTCTTAACCAGCTCCGGCACTTCCGGCGGCGAGAGAAAGCTAATGCCCACCATCCACGAAGACCTCCACCGCCGCTCTCTCTTATACAGTTTGCTTATGCCTGTCATTTCCCAGTTCCTCCCTGGCTTGGAAAACGGTAAAGGAATGTACTTTTTGTTCGTCAAAGCCGAATCTCGCACCCCAGGCGGCCTTCTCGCTCGACCCGTTTTGACTAGCTATTACAAAAGCTCCCACTTTAAGGACCGCCCCTTCGATCCTTACACCAATTACACCAGCCCCAATCAGGCTATTCTTTGCCCTGACTCCTACCAAAGCATGTACGCTCAATTGCTCTCCGGCCTCTCCCAACGTCTCCACGTCCTTCGCGTCGGCGCCGTTTTCGCCTCCGGCTTCATTCGCGCCATTCGCTTCCTCGAAAACCACTGGCAGCTTCTCTGCCACGACATCCGCACTGGGACGCTCAATTCCCAAATCACCGACCAAGCGGTTCGCGATGCAGTGATGTCACTCTTGCCTAGACCAGATCCTGAGCTAGCCGATTTTATTCATGGGGAATGTTGTAAGGGGTCATGGCAGGGAATTATTACGAGGCTATGGCCTAATACCAAGTATGTGGATGTCATTGTTACTGGGACAATGTCGCAGTATATTGGGACGCTTGATTATTATAGCAATGGACTGCCTTTGGTTTGTACCATGTATGCTTCTTCCGAGTGCTATTTTGGCGTCAATCTTAACCCTCTTTCTAAACCAACTGAAGTTGCTTATACTCTCATCCCTTCCATGGCCTATTTTGAATTCCTTCCTCTTCAAAGAAACCACAACCACGAATCCATCGACGAGCAGCACCTCGTCGATCTCACCGATGTCGAACTCGGCAAAGAATATGAGCTCGTCGTCACCACTTATGCTGGTAAACCATTCCCTCTCCCTCTCCCTCTCCCTTTATATTCTATTCAATCAAAAGAGAAAGAATAAAAGGCAACTCTCTTTTTAGCTTATGAATCTTTAAAATTGAAAGATTACATATTTATATATATAAACTTTATACCTATGTTCATGTTTATTTATGATTTATGATCTTGCATTAGAGAAAACTCCTAGTTATTGATTTGACAATTGCTATCGAGACAACACTCAAGTATTACCTATTCTTAAATTAAAAAATCATTATACATATGATAAAATTTTGGTTTCTATTATAAAAATTTCTAGGTTGGCTACTGCATCTAACATGGGGTTGAATTGACAGGATTGTATCGCTATCGAGTTGGGGACATTCTAAGAGTGGCAGGATTCAAAAACAAAGCTCCACAATTCAACTTCATCTGTCGAAAGAATGTGGTTCTAAGCATTGATTCGGACAAGACGGACGAAGTGGAGCTCCAAAATGCAGTGAAAAACTCGGTGAACCATTTGGTGCCATTTGAGGCAACATTGGCGGAGTACACAAGCTACGCAAACACTTCAACAAATCCAGGACACTACGTGCTGTATTGGGAGGTAAACGAGAAGGGGTCGACCCCGGTGCCCCCGTCGGTGTTCGAAGATTGTTGTCTTACGATGGAGGAGTCGCTGAACAGCGTATACCGTCAAGGGCGGGTGTCGGACAAGTCGATCGGGCCACTGGAGATTAAGGTTGTGGAAACTGGGACATTTGACAAGCTGATGGATTATGCCATAAGCATGGGAGCTTCTATAAATCAGTACAAAACACCTAGATGTGTGAAGTTTGAACCCATTGTTGAGCTTTTGAACTCAAGAGTGGTGGCTACTTATTTTAGCCCTAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGTCCCATCAGGATTGAAGCAGGAAGATTTTTATGTGCCTTTTCTTTTTGTTCCATGCTAGCTGTTGTTTTTTTTTTCACCCCTTGCAAGAAAATTAATAATGTGTATGTTCTTGTGATGATCTCATGTTAGAATTAGGCCAATCATCTTTTTTTGTTAGGTTTTTTTTTTCTTTTTTCAATCTCTGTTTTGGATATAGATCCTTCTGAGTAAGCCCA

mRNA sequence

ATGCCTGAAGCACCAAACACAATCTCTGTGGAAAAGAACAAGGCGAGTCTGGAATACATCGAAGAAATAACGAGGAACGCCGGAAATGTCCAGCGCCAAGTTCTGTCTCAAATCCTGTCTCGAAATGCCTCCTCCGAGTACTTAACCCTCCATGGGCGTCCTAATTCCCCTGAGACCTTCAAATCATCCATTCCTTTAATCTCTTACAATGAAATAGAACCCTTCATCACCCGCATCGCCAATGGCGATACTTCCCCTATTCTTTGCTCTTCCCCGATCTCCGAATTCTTAACCAACCTCCACCGCCGCTCTCTCTTATACAGTTTGCTTATGCCTGTCATTTCCCAGTTCCTCCCTGGCTTGGAAAACGGTAAAGGAATGTACTTTTTGTTCGTCAAAGCCGAATCTCGCACCCCAGGCGGCCTTCTCGCTCGACCCGTTTTGACTAGCTATTACAAAAGCTCCCACTTTAAGGACCGCCCCTTCGATCCTTACACCAATTACACCAGCCCCAATCAGGCTATTCTTTGCCCTGACTCCTACCAAAGCATGTACGCTCAATTGCTCTCCGGCCTCTCCCAACGTCTCCACGTCCTTCGCGTCGGCGCCGTTTTCGCCTCCGGCTTCATTCGCGCCATTCGCTTCCTCGAAAACCACTGGCAGCTTCTCTGCCACGACATCCGCACTGGGACGCTCAATTCCCAAATCACCGACCAAGCGGTTCGCGATGCAGTGATGTCACTCTTGCCTAGACCAGATCCTGAGCTAGCCGATTTTATTCATGGGGAATGTTGTAAGGGGTCATGGCAGGGAATTATTACGAGGCTATGGCCTAATACCAAGTATGTGGATGTCATTGTTACTGGGACAATGTCGCAGTATATTGGGACGCTTGATTATTATAGCAATGGACTGCCTTTGGTTTGTACCATGTATGCTTCTTCCGAGTGCTATTTTGGCGTCAATCTTAACCCTCTTTCTAAACCAACTGAAGTTGCTTATACTCTCATCCCTTCCATGGCCTATTTTGAATTCCTTCCTCTTCAAAGAAACCACAACCACGAATCCATCGACGAGCAGCACCTCGTCGATCTCACCGATGTCGAACTCGGCAAAGAATATGAGCTCGTCGTCACCACTTATGCTGGATTGTATCGCTATCGAGTTGGGGACATTCTAAGAGTGGCAGGATTCAAAAACAAAGCTCCACAATTCAACTTCATCTGTCGAAAGAATGTGGTTCTAAGCATTGATTCGGACAAGACGGACGAAGTGGAGCTCCAAAATGCAGTGAAAAACTCGGTGAACCATTTGGTGCCATTTGAGGCAACATTGGCGGAGTACACAAGCTACGCAAACACTTCAACAAATCCAGGACACTACGTGCTGTATTGGGAGGTAAACGAGAAGGGGTCGACCCCGGTGCCCCCGTCGGTGTTCGAAGATTGTTGTCTTACGATGGAGGAGTCGCTGAACAGCGTATACCGTCAAGGGCGGGTGTCGGACAAGTCGATCGGGCCACTGGAGATTAAGGTTGTGGAAACTGGGACATTTGACAAGCTGATGGATTATGCCATAAGCATGGGAGCTTCTATAAATCAGTACAAAACACCTAGATGTGTGAAGTTTGAACCCATTGTTGAGCTTTTGAACTCAAGAGTGGTGGCTACTTATTTTAGCCCTAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGTCCCATCAGGATTGAAGCAGGAAGATTTTTATGTGCCTTTTCTTTTTGTTCCATGCTAGCTGTTGTTTTTTTTTTCACCCCTTGCAAGAAAATTAATAATGTGTATGTTCTTGTGATGATCTCATGTTAGAATTAGGCCAATCATCTTTTTTTGTTAGGTTTTTTTTTTCTTTTTTCAATCTCTGTTTTGGATATAGATCCTTCTGAGTAAGCCCA

Coding sequence (CDS)

ATGCCTGAAGCACCAAACACAATCTCTGTGGAAAAGAACAAGGCGAGTCTGGAATACATCGAAGAAATAACGAGGAACGCCGGAAATGTCCAGCGCCAAGTTCTGTCTCAAATCCTGTCTCGAAATGCCTCCTCCGAGTACTTAACCCTCCATGGGCGTCCTAATTCCCCTGAGACCTTCAAATCATCCATTCCTTTAATCTCTTACAATGAAATAGAACCCTTCATCACCCGCATCGCCAATGGCGATACTTCCCCTATTCTTTGCTCTTCCCCGATCTCCGAATTCTTAACCAACCTCCACCGCCGCTCTCTCTTATACAGTTTGCTTATGCCTGTCATTTCCCAGTTCCTCCCTGGCTTGGAAAACGGTAAAGGAATGTACTTTTTGTTCGTCAAAGCCGAATCTCGCACCCCAGGCGGCCTTCTCGCTCGACCCGTTTTGACTAGCTATTACAAAAGCTCCCACTTTAAGGACCGCCCCTTCGATCCTTACACCAATTACACCAGCCCCAATCAGGCTATTCTTTGCCCTGACTCCTACCAAAGCATGTACGCTCAATTGCTCTCCGGCCTCTCCCAACGTCTCCACGTCCTTCGCGTCGGCGCCGTTTTCGCCTCCGGCTTCATTCGCGCCATTCGCTTCCTCGAAAACCACTGGCAGCTTCTCTGCCACGACATCCGCACTGGGACGCTCAATTCCCAAATCACCGACCAAGCGGTTCGCGATGCAGTGATGTCACTCTTGCCTAGACCAGATCCTGAGCTAGCCGATTTTATTCATGGGGAATGTTGTAAGGGGTCATGGCAGGGAATTATTACGAGGCTATGGCCTAATACCAAGTATGTGGATGTCATTGTTACTGGGACAATGTCGCAGTATATTGGGACGCTTGATTATTATAGCAATGGACTGCCTTTGGTTTGTACCATGTATGCTTCTTCCGAGTGCTATTTTGGCGTCAATCTTAACCCTCTTTCTAAACCAACTGAAGTTGCTTATACTCTCATCCCTTCCATGGCCTATTTTGAATTCCTTCCTCTTCAAAGAAACCACAACCACGAATCCATCGACGAGCAGCACCTCGTCGATCTCACCGATGTCGAACTCGGCAAAGAATATGAGCTCGTCGTCACCACTTATGCTGGATTGTATCGCTATCGAGTTGGGGACATTCTAAGAGTGGCAGGATTCAAAAACAAAGCTCCACAATTCAACTTCATCTGTCGAAAGAATGTGGTTCTAAGCATTGATTCGGACAAGACGGACGAAGTGGAGCTCCAAAATGCAGTGAAAAACTCGGTGAACCATTTGGTGCCATTTGAGGCAACATTGGCGGAGTACACAAGCTACGCAAACACTTCAACAAATCCAGGACACTACGTGCTGTATTGGGAGGTAAACGAGAAGGGGTCGACCCCGGTGCCCCCGTCGGTGTTCGAAGATTGTTGTCTTACGATGGAGGAGTCGCTGAACAGCGTATACCGTCAAGGGCGGGTGTCGGACAAGTCGATCGGGCCACTGGAGATTAAGGTTGTGGAAACTGGGACATTTGACAAGCTGATGGATTATGCCATAAGCATGGGAGCTTCTATAAATCAGTACAAAACACCTAGATGTGTGAAGTTTGAACCCATTGTTGAGCTTTTGAACTCAAGAGTGGTGGCTACTTATTTTAGCCCTAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGTCCCATCAGGATTGA

Protein sequence

MPEAPNTISVEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRPNSPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTNLHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQLLCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMAYFEFLPLQRNHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTSTNPGHYVLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQWSHQD
Homology
BLAST of Tan0011096 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 1.1e-256
Identity = 440/610 (72.13%), Postives = 503/610 (82.46%), Query Frame = 0

Query: 1   MPEAPNTISV--------EKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHG 60
           MPEAP   ++        EKNK  L++IE++T NA +VQR+VL +ILSRNA  EYL  HG
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  RP--NSPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN----------- 120
                  ETFK  +P+++Y +I+P I RIANGD S +LCS+PISEFLT+           
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 ------LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYK 180
                 L RRSLLYSLLMPV+ QF+PGL+ GKGMYFLF+K+ES+TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAI 240
           SSHFK+RP+DPYTNYTSPNQ ILC DSYQSMY+Q+L GL Q   VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLENHWQLLCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGII 300
           +FLE HW  L  DIRTGTL+S+ITD +VR+AV  +L +PDP+LADF+  EC K SWQGII
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGII 300

Query: 301 TRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVA 360
           TRLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PL KP+EV+
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVS 360

Query: 361 YTLIPSMAYFEFLPLQRNHNHESI----------DEQHLVDLTDVELGKEYELVVTTYAG 420
           YTLIP+MAYFEFLP+ RN    S           ++Q LVDL DV+LG+EYELVVTTYAG
Sbjct: 361 YTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 420

Query: 421 LYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEA 480
           LYRYRVGD+L VAGFKN APQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HLVPF+A
Sbjct: 421 LYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDA 480

Query: 481 TLAEYTSYANTSTNPGHYVLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDK 540
           +L+EYTSYA+TS+ PGHYVL+WE+   G+TP+PPSVFEDCCLT+EESLNSVYRQGRVSDK
Sbjct: 481 SLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDK 540

Query: 541 SIGPLEIKVVETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKC 574
           SIGPLEIK+VE+GTFDKLMDYAIS+GASINQYKTPRCVKF PI+ELLNSRVV +YFSPKC
Sbjct: 541 SIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKC 600

BLAST of Tan0011096 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 1.5e-255
Identity = 447/611 (73.16%), Postives = 503/611 (82.32%), Query Frame = 0

Query: 1   MPEAPNTISVE--------KNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLH- 60
           MPEAP   S+E        KNK  L+ IEE+T NA  VQRQVL +IL+RNA  EYL  H 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  --GRPNSPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN---------- 120
             GR +  ETFK+ +P+I+Y +IEP I RIANGD SPIL S PISEFLT+          
Sbjct: 61  LNGRTDR-ETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKL 120

Query: 121 -------LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYY 180
                  L RRSLLYSLLMPV+SQF+PGLENGKGMYFLF+K+ES+TPGGL ARPVLTSYY
Sbjct: 121 MPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYY 180

Query: 181 KSSHFKDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRA 240
           KSSHFK+RP+DPYTNYTSPN+ ILC DSYQSMY+Q+L GL Q   VLRVGAVFASGFIRA
Sbjct: 181 KSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRA 240

Query: 241 IRFLENHWQLLCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGI 300
           I+FLE HW  L  DIRTGTL+S ITD +VR+AV  +L +P P+LADF+  EC K SWQGI
Sbjct: 241 IKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEV 360
           ITRLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PL KP+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPLQRNHN-HESI---------DEQHLVDLTDVELGKEYELVVTTYA 420
           +YTLIPSMAYFEFLP+ RN+    SI         ++Q LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFE 480
           GL RYRVGD+LRV GFKNKAPQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HLVPF+
Sbjct: 421 GLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 ATLAEYTSYANTSTNPGHYVLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSD 540
           A+L+EYTSYA+TS+ PGHYVL+WE+   G+TP+PPSVFEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 541 KSIGPLEIKVVETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPK 574
           KSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVKF PI+ELLNSRVV +YFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

BLAST of Tan0011096 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 4.8e-225
Identity = 389/601 (64.73%), Postives = 461/601 (76.71%), Query Frame = 0

Query: 1   MPEAPNTISVE-------KNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGR 60
           MPEAP   +          +  +LE+IE +T NAG VQR+VL +IL++NA +EYL  +G 
Sbjct: 1   MPEAPTAKTAPAYGYAPGAHAEALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGI 60

Query: 61  PNSP---ETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN----------- 120
           P SP   + F+  +PL++Y  ++P I RIANGDTSPI    PISEFLT+           
Sbjct: 61  PGSPDVVDAFRRLVPLVTYEGLQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLM 120

Query: 121 ------LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYK 180
                 ++RRSLLYSLLMPV+SQ + GL+ GK MY LFVKAESRTPGGL ARPVLTSYY+
Sbjct: 121 PTIADEMNRRSLLYSLLMPVMSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYR 180

Query: 181 SSHFKDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAI 240
           S  F DRP DPYT+YTSP++AILC DSYQSMYAQLL GL  R  VLRVGAVFASGF+RAI
Sbjct: 181 SRQFLDRPRDPYTSYTSPDEAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAI 240

Query: 241 RFLENHWQLLCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGII 300
            FLE HW  LCHDIRTG L+ +ITD+ VRDAV  +L R DP LAD I  EC + SW+GII
Sbjct: 241 HFLEKHWARLCHDIRTGELDPEITDRVVRDAVGRVL-RADPALADAIEDECARASWEGII 300

Query: 301 TRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVA 360
            RLWP TKY+DVIVTGTMSQYI TL++Y  GLPL CTMYASSECYFG+NLNP+ KP++VA
Sbjct: 301 RRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVA 360

Query: 361 YTLIPSMAYFEFLPLQRNHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDIL 420
           YTLIP+M Y+EFLP+  N+       + LVDL DV+LG EYELVVTTY+GLYRYRVGD+L
Sbjct: 361 YTLIPTMCYYEFLPVNCNNATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVL 420

Query: 421 RVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYAN 480
           RVAGFKNKAP F+F+ R+NV LS+DSDKTDE EL  AV  +V HL PF A+L EYTSYA+
Sbjct: 421 RVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYAD 480

Query: 481 TSTNPGHYVLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVV 540
            +T PGHYVL+WE+   GST VP SVFE+CCL++EE+LNSVYRQGR  D+SIGPLEI+VV
Sbjct: 481 AATIPGHYVLFWEL-RAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVV 540

Query: 541 ETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQW 575
             GTFDKLMDYAIS GASINQYK PRCV+  P+VELL++RV   YFSPKCPKW  G+KQW
Sbjct: 541 AEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQW 599

BLAST of Tan0011096 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 2.0e-215
Identity = 378/597 (63.32%), Postives = 449/597 (75.21%), Query Frame = 0

Query: 4   APNTISVEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHG----RPNSPET 63
           A  T    +++  LE+IE +TRNAG  QR+VL +IL++NA +EYL   G     P + E 
Sbjct: 14  AAATAMCAEHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEA 73

Query: 64  FKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN-----------------LHR 123
           F+   PL++Y +I P + RIANGDTSPIL   P+SEFLT+                 + R
Sbjct: 74  FRRLAPLVTYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMER 133

Query: 124 RSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPF 183
           RS LYSLLMPV+S+ +PGL+ GK MY  FVK+E RTPGGL ARPVLTS+Y+S +F +RP 
Sbjct: 134 RSGLYSLLMPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPH 193

Query: 184 DPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQL 243
           DPYT YTSP++A+LC D+YQSMYAQL+ GL  R  VLRVGAVFASGF+RAIRFLE HW  
Sbjct: 194 DPYTVYTSPDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPS 253

Query: 244 LCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTKY 303
           LC DIR G L+  +TD AVR AV  +L   DP LAD I  EC + SWQGII R+WP+TKY
Sbjct: 254 LCRDIRAGELDGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKY 313

Query: 304 VDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMAY 363
           +DVIVTG M+QYI TL++Y  GLPL CTMYASSECYFG+NLNP+ KP+EVAYTLIP+M Y
Sbjct: 314 IDVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCY 373

Query: 364 FEFLPLQRNHNHESI---DEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFK 423
           FEFLP+    N  +    D + LVDL DV+LG EYELVVTTY+GLYRYRVGD+LRVAGFK
Sbjct: 374 FEFLPVNSGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFK 433

Query: 424 NKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTSTN-P 483
           N AP F F+ RKNV LSIDSDKTDE EL  AV  +V HL PF A+L EYTSYA+T+T  P
Sbjct: 434 NAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIP 493

Query: 484 GHYVLYWEV-NEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGT 543
           GHYVL+WE+ +  G TPVP SVFEDCCL +EE LNSVYRQ R +D+SIGPLEI+VV  GT
Sbjct: 494 GHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGT 553

Query: 544 FDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVA-GHKQW 574
           FDKLMDYA+S GASINQYK PRCV+  P+VELL+ RV ATYFSPKCPKW A G+KQW
Sbjct: 554 FDKLMDYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQW 610

BLAST of Tan0011096 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 1.4e-203
Identity = 359/578 (62.11%), Postives = 438/578 (75.78%), Query Frame = 0

Query: 11  EKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYL---TLHGRPNSPETFKSSIPLI 70
           EK+  +L +IEE+TRNA  VQ  +L++IL+RNA +EYL    L G  +  +TFK+ IP+I
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDR-DTFKTKIPVI 76

Query: 71  SYNEIEPFITRIANGDTSPILCSSPISEFLTN-----------------LHRRSLLYSLL 130
           +Y +++P I RIA+GD SPIL + PISEFLT+                 L RR LLYSLL
Sbjct: 77  TYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLL 136

Query: 131 MPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPFDPYTNYTS 190
           MPV++ ++PGL+ GKGMYFLFVK+E++TPGGL ARPVLTSYYKS HF+ RP+DPY  YTS
Sbjct: 137 MPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 191 PNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQLLCHDIRTG 250
           PN+AILCPDS+QSMY Q+L GL  RL VLRVGAVFASG +RAIRFL+ HW    HDI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 251 TLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTKYVDVIVTGT 310
            L+S+ITD ++R  +  +L +PDP LA+FI  EC   +W+ IITR+WPNTKY+DVIVTG 
Sbjct: 257 CLDSEITDPSIRQCMSGIL-KPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGA 316

Query: 311 MSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMAYFEFLPLQR 370
           M+QYI TL+YYS GLP+ CTMYASSECYFG+NLNP+SKP+EV+YT++P+MAYFEF+PL  
Sbjct: 317 MAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPL-- 376

Query: 371 NHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFICR 430
                       V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF N APQF+F+ R
Sbjct: 377 -------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRR 436

Query: 431 KNVVLSIDSDKTDEVELQNAVKNSVNHL-VPFEATLAEYTSYANTSTNPGHYVLYWEVNE 490
           KNV+LSIDSDKTDE ELQ AV+N+ + L     + +AEYTSYA+TST PGHYVLYWE+  
Sbjct: 437 KNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLV 496

Query: 491 KGSTPVPP-SVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKLMDYAISM 550
           +     P       CCL MEESLNSVYRQ RV+D S+GPLEI+VV  GTF++LMDYAIS 
Sbjct: 497 RDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISR 556

Query: 551 GASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKW 567
           GASINQYK PRCV F PIVELL+SRVV+ +FSP  P W
Sbjct: 557 GASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHW 583

BLAST of Tan0011096 vs. NCBI nr
Match: XP_038905526.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida])

HSP 1 Score: 1034.6 bits (2674), Expect = 3.1e-298
Identity = 509/603 (84.41%), Postives = 552/603 (91.54%), Query Frame = 0

Query: 1   MPEAPNTIS-----VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRPN 60
           MPEAP T +     V+KNKA L++IE++T NA  VQRQVLSQILSRN++SEYLTL+GRP 
Sbjct: 1   MPEAPKTATPNYNLVQKNKAILQFIEDVTTNAALVQRQVLSQILSRNSNSEYLTLYGRP- 60

Query: 61  SPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN---------------- 120
           SP+TFK+SIPL+SY +I+PF++RIANGD SPILCSSPISEFLT+                
Sbjct: 61  SPDTFKTSIPLVSYEQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEE 120

Query: 121 -LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFK 180
            L RRSLLYSLLMPV++QF+PGLE GKGMYFLFVKAE++TPGGLLARPVLTSYYKSSHFK
Sbjct: 121 ELERRSLLYSLLMPVMTQFVPGLEKGKGMYFLFVKAEAKTPGGLLARPVLTSYYKSSHFK 180

Query: 181 DRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLEN 240
           DRP+DPYTNYTSPN+AILCPDSYQSMYAQLL GL  RL+VLRVGAVFASGFIRAIRFLE 
Sbjct: 181 DRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLNVLRVGAVFASGFIRAIRFLEK 240

Query: 241 HWQLLCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGIITRLWP 300
           HWQLLCHDIRTGTLNSQITDQ VRDAVM +L RPDPELAD++HGECCKGSWQGIITRLWP
Sbjct: 241 HWQLLCHDIRTGTLNSQITDQEVRDAVMLVLRRPDPELADYVHGECCKGSWQGIITRLWP 300

Query: 301 NTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIP 360
           NTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPL KP+EVAYTLIP
Sbjct: 301 NTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIP 360

Query: 361 SMAYFEFLPLQRNH--NHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVA 420
           SMAYFEFLP++RNH  +++S+DEQ LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVA
Sbjct: 361 SMAYFEFLPVERNHKGDNKSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVA 420

Query: 421 GFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTST 480
           GFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS NHLVPFEATLAEYTSYANTST
Sbjct: 421 GFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSANHLVPFEATLAEYTSYANTST 480

Query: 481 NPGHYVLYWEVNEKG--STPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVE 540
            PGHYVLYWE+N+KG  +TPVPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKVVE
Sbjct: 481 IPGHYVLYWELNQKGGSTTPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVE 540

Query: 541 TGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQWS 578
           TGTFDKLMDYAISMGASINQYKTPRCVKF+PIVELL+SRV+A+YFSPKCPKWV GHKQWS
Sbjct: 541 TGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLDSRVLASYFSPKCPKWVPGHKQWS 600

BLAST of Tan0011096 vs. NCBI nr
Match: XP_022942727.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1028.1 bits (2657), Expect = 2.9e-296
Identity = 510/596 (85.57%), Postives = 540/596 (90.60%), Query Frame = 0

Query: 1   MPEAPNTIS-VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRPNSPET 60
           MPEAPN  S +EKN A+L++IE+IT NA +VQRQVLSQILSRNASSEYLTLH RP SP+T
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN-----------------LHR 120
           FKSSIPLISY+ I+PFITRIANGDTSPILCSSPISEFLT+                 L R
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPF 180
           RSLLYSLLMPV++QF+PGLE GKGMYFLFVKAE+RTPGGLLARPVLTSYYKSSHFKDRP+
Sbjct: 121 RSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQL 240
           DPYTNYTSPN+AILC DSYQSMYAQ+L GL  RL VLRVGAVFASGFIRAIRFLE HWQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSQITDQAVRDAV-MSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTK 300
           LCHDIR+GTLNSQITDQAVR+AV MS+L RPDPEL DFI GECCKGSWQGIITRLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYSNGLPLVC+MYASSECYFG+NLNPL  P EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPLQRNHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 420
           YFEFLPLQ NHNH    E  LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK
Sbjct: 361 YFEFLPLQTNHNH----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 420

Query: 421 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTSTNPGHY 480
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPF+ATL +YTSYANTST PGHY
Sbjct: 421 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHY 480

Query: 481 VLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKL 540
           V+YWEVN+KGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKL
Sbjct: 481 VVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKL 540

Query: 541 MDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQWSHQD 578
           MDYAI+MGASINQYKTPRCVKFEPIV+LLNSRVV +YFSPKCPKWVAGHKQW  QD
Sbjct: 541 MDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

BLAST of Tan0011096 vs. NCBI nr
Match: XP_022985796.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1026.5 bits (2653), Expect = 8.6e-296
Identity = 510/596 (85.57%), Postives = 538/596 (90.27%), Query Frame = 0

Query: 1   MPEAPNTIS-VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRPNSPET 60
           MPEAPN  S VEKN A+L++IE IT NA +VQRQVLSQILSRNASSEYLTLH RP SP+T
Sbjct: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN-----------------LHR 120
           FKSSIPLISY+ I+PFITRIANGDTSPILCSSPISEFLT+                 L R
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPF 180
           RSLLYSLLMPV++QF+PGLE GKGMYFLFVKAE+RTPGGLLARPVLTSYYKSSHFKDRP+
Sbjct: 121 RSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQL 240
           DPYTNYTSPN+AILC DSYQSMYAQ+L GL  RL VLRVGAVFASGFIRAIRFLE HWQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSQITDQAVRDAV-MSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTK 300
           LCHDIR+GTLNSQITDQAVR+AV MS+L RPDPEL DFI GECCKGSWQGIITRLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYSNGLPLVC+MYASSECYFG+NLNPL  P EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPLQRNHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 420
           YFEFLPLQ NHNH    E  LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 
Sbjct: 361 YFEFLPLQTNHNH----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNN 420

Query: 421 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTSTNPGHY 480
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPF+ATL +YTSYANTST PGHY
Sbjct: 421 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHY 480

Query: 481 VLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKL 540
           V+YWEVN+KGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKL
Sbjct: 481 VVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKL 540

Query: 541 MDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQWSHQD 578
           MDYAI+MGASINQYKTPRCVKFEPIV+LLNSRVV +YFSPKCPKWVAGHKQW  QD
Sbjct: 541 MDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

BLAST of Tan0011096 vs. NCBI nr
Match: XP_008452341.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA0047007.1 indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo var. makuwa] >TYK04773.1 indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo var. makuwa])

HSP 1 Score: 1026.2 bits (2652), Expect = 1.1e-295
Identity = 508/605 (83.97%), Postives = 550/605 (90.91%), Query Frame = 0

Query: 1   MPEAPNTIS------VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRP 60
           MPEAP   +      V+KNKA L++IE++T NA  VQRQVLSQILS+N++SEYLTL+GRP
Sbjct: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60

Query: 61  NSPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN--------------- 120
            SP+TFK+SIPL+SY++I+PF++RIANGD SPILCSSPISEFLT+               
Sbjct: 61  -SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIE 120

Query: 121 --LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHF 180
             L RRSLLYSLLMPV+SQF+PGLE GKGMYFLF+KAE++TPGGLLARPVLTSYYKSSHF
Sbjct: 121 EELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHF 180

Query: 181 KDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLE 240
           K+RP+DPYTNYTSPN+AILCPDSYQSMYAQLL GL  RL VLRVGAVFASGFIRAIRFLE
Sbjct: 181 KERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLE 240

Query: 241 NHWQLLCHDIRTGTLNSQITDQAVRDAVM-SLLPRPDPELADFIHGECCKGSWQGIITRL 300
            HWQLLCHDIRTGTLNSQITDQAVRDAVM S+L RPDPELAD+IHGECCKGSWQGIITRL
Sbjct: 241 KHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRL 300

Query: 301 WPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTL 360
           WPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPL KP+EVAYTL
Sbjct: 301 WPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTL 360

Query: 361 IPSMAYFEFLPLQRNHNHE--SIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420
           IPSMAYFEFLP++R+HN++  S+DEQ LVDLTDVELGKEYELVVTTYAGLYRYRVGDILR
Sbjct: 361 IPSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420

Query: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANT 480
           VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFEATLAEYTSYANT
Sbjct: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANT 480

Query: 481 STNPGHYVLYWEVNEK--GSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKV 540
           ST PGHYVLYWEVN+K   +TPVPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKV
Sbjct: 481 STIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKV 540

Query: 541 VETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQ 578
           VE GTFDKLMDYAISMGASINQYKTPRCVKF+PIVELLNSRVV +YFSPKCPKWV GHKQ
Sbjct: 541 VENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQ 600

BLAST of Tan0011096 vs. NCBI nr
Match: KAG6600750.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1025.8 bits (2651), Expect = 1.5e-295
Identity = 508/596 (85.23%), Postives = 539/596 (90.44%), Query Frame = 0

Query: 1   MPEAPNTIS-VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRPNSPET 60
           MPEAPN  S +EKN A+L++IE+IT NA +VQRQVLSQILSRNASSEYLTLH RP SP+T
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN-----------------LHR 120
           FKSSIPLISY+ I+PFITRIANGDTSPILCSSPISEFLT+                 L R
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPF 180
           RSLLYSLLMPV++QF+PGLE GKGMYFLFVKAE+RTPGGLLARPVLTSYYKSSHFKDRP+
Sbjct: 121 RSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQL 240
           DPYTNYTSPN+AILC DSYQSMYAQ+L GL  RL VLRVGAVFASGFIRAIRFLE HWQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSQITDQAVRDAV-MSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTK 300
           LCHDIR+GTLNSQITDQAVR+AV MS+L RPDPEL DFI GECCKGSWQGIITRLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYSNGLPLVC+MYASSECYFG+NLNPL  P EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPLQRNHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 420
           YFEFLPLQ NHNH    E  LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 
Sbjct: 361 YFEFLPLQTNHNH----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNN 420

Query: 421 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTSTNPGHY 480
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATL +YTSYANTST PGHY
Sbjct: 421 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHY 480

Query: 481 VLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKL 540
           V+YWEVN+KGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKL
Sbjct: 481 VVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKL 540

Query: 541 MDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQWSHQD 578
           MDYAI+MGASINQYKTPRCVKFEPIV+LLNSRVV +YFSPKCPKWVAGHKQW  QD
Sbjct: 541 MDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

BLAST of Tan0011096 vs. ExPASy TrEMBL
Match: A0A6J1FR29 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111447677 PE=3 SV=1)

HSP 1 Score: 1028.1 bits (2657), Expect = 1.4e-296
Identity = 510/596 (85.57%), Postives = 540/596 (90.60%), Query Frame = 0

Query: 1   MPEAPNTIS-VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRPNSPET 60
           MPEAPN  S +EKN A+L++IE+IT NA +VQRQVLSQILSRNASSEYLTLH RP SP+T
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN-----------------LHR 120
           FKSSIPLISY+ I+PFITRIANGDTSPILCSSPISEFLT+                 L R
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPF 180
           RSLLYSLLMPV++QF+PGLE GKGMYFLFVKAE+RTPGGLLARPVLTSYYKSSHFKDRP+
Sbjct: 121 RSLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQL 240
           DPYTNYTSPN+AILC DSYQSMYAQ+L GL  RL VLRVGAVFASGFIRAIRFLE HWQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSQITDQAVRDAV-MSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTK 300
           LCHDIR+GTLNSQITDQAVR+AV MS+L RPDPEL DFI GECCKGSWQGIITRLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYSNGLPLVC+MYASSECYFG+NLNPL  P EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPLQRNHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 420
           YFEFLPLQ NHNH    E  LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK
Sbjct: 361 YFEFLPLQTNHNH----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 420

Query: 421 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTSTNPGHY 480
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPF+ATL +YTSYANTST PGHY
Sbjct: 421 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHY 480

Query: 481 VLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKL 540
           V+YWEVN+KGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKL
Sbjct: 481 VVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKL 540

Query: 541 MDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQWSHQD 578
           MDYAI+MGASINQYKTPRCVKFEPIV+LLNSRVV +YFSPKCPKWVAGHKQW  QD
Sbjct: 541 MDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

BLAST of Tan0011096 vs. ExPASy TrEMBL
Match: A0A6J1J981 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111483735 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 4.1e-296
Identity = 510/596 (85.57%), Postives = 538/596 (90.27%), Query Frame = 0

Query: 1   MPEAPNTIS-VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRPNSPET 60
           MPEAPN  S VEKN A+L++IE IT NA +VQRQVLSQILSRNASSEYLTLH RP SP+T
Sbjct: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARP-SPDT 60

Query: 61  FKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN-----------------LHR 120
           FKSSIPLISY+ I+PFITRIANGDTSPILCSSPISEFLT+                 L R
Sbjct: 61  FKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDR 120

Query: 121 RSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPF 180
           RSLLYSLLMPV++QF+PGLE GKGMYFLFVKAE+RTPGGLLARPVLTSYYKSSHFKDRP+
Sbjct: 121 RSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPY 180

Query: 181 DPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQL 240
           DPYTNYTSPN+AILC DSYQSMYAQ+L GL  RL VLRVGAVFASGFIRAIRFLE HWQL
Sbjct: 181 DPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQL 240

Query: 241 LCHDIRTGTLNSQITDQAVRDAV-MSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTK 300
           LCHDIR+GTLNSQITDQAVR+AV MS+L RPDPEL DFI GECCKGSWQGIITRLWPNTK
Sbjct: 241 LCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTK 300

Query: 301 YVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMA 360
           YVDVIVTG MSQYIGTLDYYSNGLPLVC+MYASSECYFG+NLNPL  P EVAYTLIPSMA
Sbjct: 301 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 360

Query: 361 YFEFLPLQRNHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 420
           YFEFLPLQ NHNH    E  LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 
Sbjct: 361 YFEFLPLQTNHNH----EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNN 420

Query: 421 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTSTNPGHY 480
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPF+ATL +YTSYANTST PGHY
Sbjct: 421 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHY 480

Query: 481 VLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKL 540
           V+YWEVN+KGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKL
Sbjct: 481 VVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKL 540

Query: 541 MDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQWSHQD 578
           MDYAI+MGASINQYKTPRCVKFEPIV+LLNSRVV +YFSPKCPKWVAGHKQW  QD
Sbjct: 541 MDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

BLAST of Tan0011096 vs. ExPASy TrEMBL
Match: A0A5D3BYD3 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00850 PE=3 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 5.4e-296
Identity = 508/605 (83.97%), Postives = 550/605 (90.91%), Query Frame = 0

Query: 1   MPEAPNTIS------VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRP 60
           MPEAP   +      V+KNKA L++IE++T NA  VQRQVLSQILS+N++SEYLTL+GRP
Sbjct: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60

Query: 61  NSPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN--------------- 120
            SP+TFK+SIPL+SY++I+PF++RIANGD SPILCSSPISEFLT+               
Sbjct: 61  -SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIE 120

Query: 121 --LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHF 180
             L RRSLLYSLLMPV+SQF+PGLE GKGMYFLF+KAE++TPGGLLARPVLTSYYKSSHF
Sbjct: 121 EELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHF 180

Query: 181 KDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLE 240
           K+RP+DPYTNYTSPN+AILCPDSYQSMYAQLL GL  RL VLRVGAVFASGFIRAIRFLE
Sbjct: 181 KERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLE 240

Query: 241 NHWQLLCHDIRTGTLNSQITDQAVRDAVM-SLLPRPDPELADFIHGECCKGSWQGIITRL 300
            HWQLLCHDIRTGTLNSQITDQAVRDAVM S+L RPDPELAD+IHGECCKGSWQGIITRL
Sbjct: 241 KHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRL 300

Query: 301 WPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTL 360
           WPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPL KP+EVAYTL
Sbjct: 301 WPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTL 360

Query: 361 IPSMAYFEFLPLQRNHNHE--SIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420
           IPSMAYFEFLP++R+HN++  S+DEQ LVDLTDVELGKEYELVVTTYAGLYRYRVGDILR
Sbjct: 361 IPSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420

Query: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANT 480
           VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFEATLAEYTSYANT
Sbjct: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANT 480

Query: 481 STNPGHYVLYWEVNEK--GSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKV 540
           ST PGHYVLYWEVN+K   +TPVPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKV
Sbjct: 481 STIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKV 540

Query: 541 VETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQ 578
           VE GTFDKLMDYAISMGASINQYKTPRCVKF+PIVELLNSRVV +YFSPKCPKWV GHKQ
Sbjct: 541 VENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQ 600

BLAST of Tan0011096 vs. ExPASy TrEMBL
Match: A0A1S3BTN3 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493400 PE=3 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 5.4e-296
Identity = 508/605 (83.97%), Postives = 550/605 (90.91%), Query Frame = 0

Query: 1   MPEAPNTIS------VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRP 60
           MPEAP   +      V+KNKA L++IE++T NA  VQRQVLSQILS+N++SEYLTL+GRP
Sbjct: 1   MPEAPKPSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRP 60

Query: 61  NSPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN--------------- 120
            SP+TFK+SIPL+SY++I+PF++RIANGD SPILCSSPISEFLT+               
Sbjct: 61  -SPDTFKTSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIE 120

Query: 121 --LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHF 180
             L RRSLLYSLLMPV+SQF+PGLE GKGMYFLF+KAE++TPGGLLARPVLTSYYKSSHF
Sbjct: 121 EELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHF 180

Query: 181 KDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLE 240
           K+RP+DPYTNYTSPN+AILCPDSYQSMYAQLL GL  RL VLRVGAVFASGFIRAIRFLE
Sbjct: 181 KERPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLE 240

Query: 241 NHWQLLCHDIRTGTLNSQITDQAVRDAVM-SLLPRPDPELADFIHGECCKGSWQGIITRL 300
            HWQLLCHDIRTGTLNSQITDQAVRDAVM S+L RPDPELAD+IHGECCKGSWQGIITRL
Sbjct: 241 KHWQLLCHDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRL 300

Query: 301 WPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTL 360
           WPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPL KP+EVAYTL
Sbjct: 301 WPNTKYVDVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTL 360

Query: 361 IPSMAYFEFLPLQRNHNHE--SIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420
           IPSMAYFEFLP++R+HN++  S+DEQ LVDLTDVELGKEYELVVTTYAGLYRYRVGDILR
Sbjct: 361 IPSMAYFEFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420

Query: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANT 480
           VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFEATLAEYTSYANT
Sbjct: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANT 480

Query: 481 STNPGHYVLYWEVNEK--GSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKV 540
           ST PGHYVLYWEVN+K   +TPVPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKV
Sbjct: 481 STIPGHYVLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKV 540

Query: 541 VETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQ 578
           VE GTFDKLMDYAISMGASINQYKTPRCVKF+PIVELLNSRVV +YFSPKCPKWV GHKQ
Sbjct: 541 VENGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQ 600

BLAST of Tan0011096 vs. ExPASy TrEMBL
Match: A0A6J1JAP2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111483268 PE=3 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 1.0e-294
Identity = 506/605 (83.64%), Postives = 548/605 (90.58%), Query Frame = 0

Query: 1   MPEAPNTIS--------VEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHG 60
           MPEAP T +        V+KN A L++IE++T NA  VQRQVLSQILSRNA+SEYL+LHG
Sbjct: 1   MPEAPKTATSVSPNYNLVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYLSLHG 60

Query: 61  RPNSPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN------------- 120
           RP SP+TFKSSIPL+SY+ I+PFI+RIANGDTSPILCSSPISEFLT+             
Sbjct: 61  RP-SPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPT 120

Query: 121 ----LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSS 180
               L RRSLLYSLLMP+++QF+PGLE GKGMYFLFVKAE+RTPGGLLARPVLTSYYKSS
Sbjct: 121 IEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSS 180

Query: 181 HFKDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRF 240
           HFK+RP+DPYTNYTSPN+AILCPD+YQS+YAQLL GL  RL VLRVGAVFASGFIRAIRF
Sbjct: 181 HFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGFIRAIRF 240

Query: 241 LENHWQLLCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGIITR 300
           LE HWQLLCHDIRTGTLNSQITDQAVRDAVM +L RPDPELADFIH +CCKG W+GIITR
Sbjct: 241 LEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLVLRRPDPELADFIHEQCCKGFWKGIITR 300

Query: 301 LWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYT 360
           LWPNTKY+DVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPL KP+EVAYT
Sbjct: 301 LWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYT 360

Query: 361 LIPSMAYFEFLPLQR-NHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420
           LIPSMAYFEFLP+QR ++N ES+D+Q LVDLTDVELGKEYELVVTTYAGLYRYRVGDILR
Sbjct: 361 LIPSMAYFEFLPVQRTSNNDESLDDQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420

Query: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANT 480
           VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVK SVNHLVPFEATLAEYTS+ANT
Sbjct: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKKSVNHLVPFEATLAEYTSHANT 480

Query: 481 STNPGHYVLYWEVNEKG--STPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKV 540
           ST PGHYVLYWE+N+KG  +TPVPPSV EDCCLT+EESLNSVYRQGRV+DKSIGPLEIKV
Sbjct: 481 STIPGHYVLYWELNQKGGSATPVPPSVLEDCCLTIEESLNSVYRQGRVADKSIGPLEIKV 540

Query: 541 VETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKWVAGHKQ 578
           VE+GTFDKLMDYAISMGASINQYKTPRCVKF+PIVELLNSRVV  YFSPKCPKWVAGHKQ
Sbjct: 541 VESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPKWVAGHKQ 600

BLAST of Tan0011096 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 887.1 bits (2291), Expect = 7.6e-258
Identity = 440/610 (72.13%), Postives = 503/610 (82.46%), Query Frame = 0

Query: 1   MPEAPNTISV--------EKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHG 60
           MPEAP   ++        EKNK  L++IE++T NA +VQR+VL +ILSRNA  EYL  HG
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  RP--NSPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN----------- 120
                  ETFK  +P+++Y +I+P I RIANGD S +LCS+PISEFLT+           
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 ------LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYK 180
                 L RRSLLYSLLMPV+ QF+PGL+ GKGMYFLF+K+ES+TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAI 240
           SSHFK+RP+DPYTNYTSPNQ ILC DSYQSMY+Q+L GL Q   VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLENHWQLLCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGII 300
           +FLE HW  L  DIRTGTL+S+ITD +VR+AV  +L +PDP+LADF+  EC K SWQGII
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGII 300

Query: 301 TRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVA 360
           TRLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PL KP+EV+
Sbjct: 301 TRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVS 360

Query: 361 YTLIPSMAYFEFLPLQRNHNHESI----------DEQHLVDLTDVELGKEYELVVTTYAG 420
           YTLIP+MAYFEFLP+ RN    S           ++Q LVDL DV+LG+EYELVVTTYAG
Sbjct: 361 YTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 420

Query: 421 LYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEA 480
           LYRYRVGD+L VAGFKN APQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HLVPF+A
Sbjct: 421 LYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDA 480

Query: 481 TLAEYTSYANTSTNPGHYVLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDK 540
           +L+EYTSYA+TS+ PGHYVL+WE+   G+TP+PPSVFEDCCLT+EESLNSVYRQGRVSDK
Sbjct: 481 SLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDK 540

Query: 541 SIGPLEIKVVETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKC 574
           SIGPLEIK+VE+GTFDKLMDYAIS+GASINQYKTPRCVKF PI+ELLNSRVV +YFSPKC
Sbjct: 541 SIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKC 600

BLAST of Tan0011096 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 883.2 bits (2281), Expect = 1.1e-256
Identity = 447/611 (73.16%), Postives = 503/611 (82.32%), Query Frame = 0

Query: 1   MPEAPNTISVE--------KNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLH- 60
           MPEAP   S+E        KNK  L+ IEE+T NA  VQRQVL +IL+RNA  EYL  H 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  --GRPNSPETFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLTN---------- 120
             GR +  ETFK+ +P+I+Y +IEP I RIANGD SPIL S PISEFLT+          
Sbjct: 61  LNGRTDR-ETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKL 120

Query: 121 -------LHRRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYY 180
                  L RRSLLYSLLMPV+SQF+PGLENGKGMYFLF+K+ES+TPGGL ARPVLTSYY
Sbjct: 121 MPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYY 180

Query: 181 KSSHFKDRPFDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRA 240
           KSSHFK+RP+DPYTNYTSPN+ ILC DSYQSMY+Q+L GL Q   VLRVGAVFASGFIRA
Sbjct: 181 KSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRA 240

Query: 241 IRFLENHWQLLCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGI 300
           I+FLE HW  L  DIRTGTL+S ITD +VR+AV  +L +P P+LADF+  EC K SWQGI
Sbjct: 241 IKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEV 360
           ITRLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PL KP+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPLQRNHN-HESI---------DEQHLVDLTDVELGKEYELVVTTYA 420
           +YTLIPSMAYFEFLP+ RN+    SI         ++Q LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFE 480
           GL RYRVGD+LRV GFKNKAPQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HLVPF+
Sbjct: 421 GLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 ATLAEYTSYANTSTNPGHYVLYWEVNEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSD 540
           A+L+EYTSYA+TS+ PGHYVL+WE+   G+TP+PPSVFEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 541 KSIGPLEIKVVETGTFDKLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPK 574
           KSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVKF PI+ELLNSRVV +YFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

BLAST of Tan0011096 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 711.4 bits (1835), Expect = 5.7e-205
Identity = 352/584 (60.27%), Postives = 443/584 (75.86%), Query Frame = 0

Query: 11  EKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRPN--SPETFKSSIPLIS 70
           EK+  +L++IEE+TRN  +VQ +VL +IL+RN+++EYL           +TFKS +P+++
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 71  YNEIEPFITRIANGDTSPILCSSPISEFLT-----------------NLHRRSLLYSLLM 130
           Y +++P I RI+NGD SPIL S PI+EFLT                 +L RR LLYSLLM
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 131 PVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPFDPYTNYTSP 190
           PV++ ++PGL+ GKG+YFLFVK+ES+T GGL ARPVLTSYYKS HFK RP+DPY  YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 191 NQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQLLCHDIRTGT 250
           N+AILC DS QSMYAQ+L GL  R  VLR+GAVFASG +RAI FL+N+W+ L  DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 251 LNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTKYVDVIVTGTM 310
           L+S+I D A+++ +  +L +PD ELA+F+ G C + +W+GIIT++WPNTKY+DVIVTG M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 311 SQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMAYFEFLPLQRN 370
           +QYI TL+YYS GLP+ CTMYASSE YFG+NL P+ KP+EV+YT++P+MAYFEFLP    
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP---- 377

Query: 371 HNHE-------SIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQ 430
           HNH+       S+DE  LV+L +VE+GKEYELV+TTYAGLYRYRVGDILRV GF N APQ
Sbjct: 378 HNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQ 437

Query: 431 FNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTSTNPGHYVLY 490
           F FI RKNV+LS++SDKTDE ELQ AV+N+          + EYTSYA T T PGHYV+Y
Sbjct: 438 FKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIY 497

Query: 491 WEV--NEKGSTPVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKLM 550
           WE+   ++ +  +   V   CCL MEESLNSVYRQ RV+DKSIGPLEI+VV  GTF++LM
Sbjct: 498 WELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELM 557

Query: 551 DYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKW 567
           DYAIS GASINQYK PRCV F PI+ELL+SRVV+ +FSP  P W
Sbjct: 558 DYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHW 597

BLAST of Tan0011096 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 710.7 bits (1833), Expect = 9.7e-205
Identity = 359/578 (62.11%), Postives = 438/578 (75.78%), Query Frame = 0

Query: 11  EKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYL---TLHGRPNSPETFKSSIPLI 70
           EK+  +L +IEE+TRNA  VQ  +L++IL+RNA +EYL    L G  +  +TFK+ IP+I
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDR-DTFKTKIPVI 76

Query: 71  SYNEIEPFITRIANGDTSPILCSSPISEFLTN-----------------LHRRSLLYSLL 130
           +Y +++P I RIA+GD SPIL + PISEFLT+                 L RR LLYSLL
Sbjct: 77  TYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLL 136

Query: 131 MPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRPFDPYTNYTS 190
           MPV++ ++PGL+ GKGMYFLFVK+E++TPGGL ARPVLTSYYKS HF+ RP+DPY  YTS
Sbjct: 137 MPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 191 PNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQLLCHDIRTG 250
           PN+AILCPDS+QSMY Q+L GL  RL VLRVGAVFASG +RAIRFL+ HW    HDI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 251 TLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCKGSWQGIITRLWPNTKYVDVIVTGT 310
            L+S+ITD ++R  +  +L +PDP LA+FI  EC   +W+ IITR+WPNTKY+DVIVTG 
Sbjct: 257 CLDSEITDPSIRQCMSGIL-KPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGA 316

Query: 311 MSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSMAYFEFLPLQR 370
           M+QYI TL+YYS GLP+ CTMYASSECYFG+NLNP+SKP+EV+YT++P+MAYFEF+PL  
Sbjct: 317 MAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPL-- 376

Query: 371 NHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFICR 430
                       V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF N APQF+F+ R
Sbjct: 377 -------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRR 436

Query: 431 KNVVLSIDSDKTDEVELQNAVKNSVNHL-VPFEATLAEYTSYANTSTNPGHYVLYWEVNE 490
           KNV+LSIDSDKTDE ELQ AV+N+ + L     + +AEYTSYA+TST PGHYVLYWE+  
Sbjct: 437 KNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLV 496

Query: 491 KGSTPVPP-SVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKLMDYAISM 550
           +     P       CCL MEESLNSVYRQ RV+D S+GPLEI+VV  GTF++LMDYAIS 
Sbjct: 497 RDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISR 556

Query: 551 GASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKW 567
           GASINQYK PRCV F PIVELL+SRVV+ +FSP  P W
Sbjct: 557 GASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHW 583

BLAST of Tan0011096 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 691.8 bits (1784), Expect = 4.7e-199
Identity = 351/587 (59.80%), Postives = 439/587 (74.79%), Query Frame = 0

Query: 1   MPEAPNTISVEKNKASLEYIEEITRNAGNVQRQVLSQILSRNASSEYLTLHGRP--NSPE 60
           M  +P+T    K+  +L +IEE+TRN   VQ++V+ +ILSRN+ +EYL   G       +
Sbjct: 11  MMHSPST----KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRK 70

Query: 61  TFKSSIPLISYNEIEPFITRIANGDTSPILCSSPISEFLT-----------------NLH 120
           TFK+ +P++ Y++++P I RIANGD S IL S PI+EFLT                 ++ 
Sbjct: 71  TFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMD 130

Query: 121 RRSLLYSLLMPVISQFLPGLENGKGMYFLFVKAESRTPGGLLARPVLTSYYKSSHFKDRP 180
           RR LLYSLLMPV++ ++PGL+ GK +YFLFVK ES+TPGGL ARPVLTSYYKS  FK RP
Sbjct: 131 RRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRP 190

Query: 181 FDPYTNYTSPNQAILCPDSYQSMYAQLLSGLSQRLHVLRVGAVFASGFIRAIRFLENHWQ 240
            DPY  YTSPN+AILCPDS QSMY Q+L GL  R  VLR+GAVFASG +RAI FL+ +W+
Sbjct: 191 NDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWK 250

Query: 241 LLCHDIRTGTLNSQITDQAVRDAVMSLLPRPDPELADFIHGECCK-GSWQGIITRLWPNT 300
            L  DI TGTL+S+I+D A+++++  +L +PD ELADFI   C +  SW+GIIT++WPNT
Sbjct: 251 ELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNT 310

Query: 301 KYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLSKPTEVAYTLIPSM 360
           KY+DVIVTG M+QYI  L+YYS GLP+ CTMYASSE YFG+NL P+ KP+EV+YT++P+M
Sbjct: 311 KYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNM 370

Query: 361 AYFEFLPLQRNHNHESIDEQHLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 420
           AYFEFLP    H+    ++  LV+L DVE+GKEYELV+TTYAGL RYRVGDIL+V GF N
Sbjct: 371 AYFEFLP----HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYN 430

Query: 421 KAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSYANTSTNPGH 480
            APQF F+ RKNV+LSI+SDKTDE ELQ+AV+N+   L      + EYTSYA T T PGH
Sbjct: 431 SAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGH 490

Query: 481 YVLYWEVNEKGST-PVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVETGTFD 540
           YV+YWE+  K  T P    V   CCL MEESLNSVYRQ RV+DKSIGPLEI+VV+ GTF+
Sbjct: 491 YVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFE 550

Query: 541 KLMDYAISMGASINQYKTPRCVKFEPIVELLNSRVVATYFSPKCPKW 567
           +LMDYAIS GASINQYK PRCV F PI+ELL+SRVV+T+FSP  P W
Sbjct: 551 ELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHW 589

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LSQ41.1e-25672.13Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
O818291.5e-25573.16Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM54.8e-22564.73Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
Q60EJ62.0e-21563.32Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... [more]
O823331.4e-20362.11Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
XP_038905526.13.1e-29884.41indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida][more]
XP_022942727.12.9e-29685.57indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata][more]
XP_022985796.18.6e-29685.57indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima][more]
XP_008452341.11.1e-29583.97PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA004700... [more]
KAG6600750.11.5e-29585.23Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
A0A6J1FR291.4e-29685.57indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1J9814.1e-29685.57indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A5D3BYD35.4e-29683.97Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BTN35.4e-29683.97indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493... [more]
A0A6J1JAP21.0e-29483.64indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
AT5G54510.17.6e-25872.13Auxin-responsive GH3 family protein [more]
AT4G27260.11.1e-25673.16Auxin-responsive GH3 family protein [more]
AT4G37390.15.7e-20560.27Auxin-responsive GH3 family protein [more]
AT2G14960.19.7e-20562.11Auxin-responsive GH3 family protein [more]
AT2G23170.14.7e-19959.80Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 20..543
e-value: 3.4E-179
score: 597.1
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 9..574
NoneNo IPR availablePANTHERPTHR31901:SF70BNAC03G14570D PROTEINcoord: 9..574

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0011096.1Tan0011096.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0010279 indole-3-acetic acid amido synthetase activity