Tan0011054 (gene) Snake gourd v1

BLAST of Tan0011054 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 6.0e-231
Identity = 411/750 (54.80%), Postives = 534/750 (71.20%), Query Frame = 0

Query: 21  VADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLDEDQEEASNAAEILYVYKTAL 80
           V+  +QTY+IH       TT+ +   TS+ +S+   +  D+D     +  EI Y+Y+ A+
Sbjct: 37  VSSRKQTYVIH-----TVTTSTKHIVTSLFNSLQTENINDDD----FSLPEIHYIYENAM 96

Query: 81  SGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQFLGLRRGHGLWNSSNMASDII 140
           SGFSA L+  +L  +    GF++A  DELL LHTT++ +FLGL  G GLWN ++++SD+I
Sbjct: 97  SGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSDVI 156

Query: 141 IGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRSNCNKKLIGARAYIKGYEAIL 200
           IG++D+GI PEH+SF+D    PVP +W+G C+ G  FS S CNKK+IGA A+ KGYE+I+
Sbjct: 157 IGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYESIV 216

Query: 201 GRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMGVAAGISFTSRIAAYKVCW 260
           G++N T  FRS RD+ GHGTHTASTAAG+IV KA+ + Q  G+A+G+ FTSRIAAYK CW
Sbjct: 217 GKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKACW 276

Query: 261 PEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEMAIATFGAVRNGVFVSCSAGN 320
             GCAS D++AAIDRA++DGVDV+SLSLGG    FY D +AIA FGA++  +FVSCSAGN
Sbjct: 277 ALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGN 336

Query: 321 SGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFKGSSLYSGNNIDQLPLVYNNT 380
           SGP  STV N APW+MTVAASYTDRTFPA +++GN +   GSSLY G ++  LPL +N T
Sbjct: 337 SGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGKSLKNLPLAFNRT 396

Query: 381 AGGEEANV-CAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQVKLAGGAGMILINIRLEGED 440
           AG E   V C   SL   +V+GKIV+C +G + R  KGE+VK +GGA M+L++   EGE+
Sbjct: 397 AGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSGGAAMLLVSTEAEGEE 456

Query: 441 FFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEGTKYRSRAPIVAAFSSRGPS 500
             ADPHVLPA++LG  S GK ++NY+A + +   AS+ F GT Y + AP+VAAFSSRGPS
Sbjct: 457 LLADPHVLPAVSLGF-SDGKTLLNYLAGAAN-ATASVRFRGTAYGATAPMVAAFSSRGPS 516

Query: 501 FFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFNIISGTSMSCPHVSGIAALL 560
              P + KPDI APG+NILA W    SP+ L SD RRV FNIISGTSM+CPH+SGIAAL+
Sbjct: 517 VAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHISGIAALI 576

Query: 561 KSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASG-GPANAFALGSGHVDPEKAVDP 620
           KSVH +WSPA IKSA+MTTA ITDN+ R I D G A     A AFA G+G+VDP +AVDP
Sbjct: 577 KSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDP 636

Query: 621 GLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTLLQPGDLNYPSFSVFMKKKA 680
           GL+YD +  DYLNYLCS NYTS+++ L S  N+TC S   +L PGDLNYPSF+V +   A
Sbjct: 637 GLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFAVNLVNGA 696

Query: 681 KNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSFRRLGQKLSYQVSFVALGK 740
               V  KRTVTNVGSP  +Y V +  P G+ + V+P+ L F++  ++LSY V++ A   
Sbjct: 697 NLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTVTYDAEAS 756

Query: 741 GKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
             + S  SFG L+W+  KY VRSPIAVTW+
Sbjct: 757 RNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Tan0011054 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 2.9e-193
Identity = 371/782 (47.44%), Postives = 493/782 (63.04%), Query Frame = 0

Query: 7   WLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPE--QWYTSMIDSVNELSSLDEDQE 66
           +  L  + + S++A + +  TYI+H+D     +  P    WYTS + S+           
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----------- 66

Query: 67  EASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQFLGLR 126
             S+   I++ Y T   GFSA+L+++    L   P  ++   +++  LHTT +P+FLGLR
Sbjct: 67  -TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLR 126

Query: 127 RGH--GLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRSNC 186
                GL   S+  SD++IGVID+G+WPE  SF D+G  PVP KWKG C   Q F  S C
Sbjct: 127 STDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESAC 186

Query: 187 NKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMG 246
           N+KL+GAR +  GYEA  G++N T  FRS RDSDGHGTHTAS +AG  V+ AS      G
Sbjct: 187 NRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 246

Query: 247 VAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEMAI 306
           VAAG++  +R+AAYKVCW  GC  +DILAA D AV DGVDV+SLS+GG    +Y D +AI
Sbjct: 247 VAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAI 306

Query: 307 ATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFKGS 366
             FGA+  G+FVS SAGN GP   TV NVAPW+ TV A   DR FPA +KLGNG++  G 
Sbjct: 307 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 366

Query: 367 SLYSGNNID---QLPLVYNNT---AGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEK 426
           S+Y G  +D     PLVY  +     G  +++C  GSL P++VKGKIV+C++G NSR  K
Sbjct: 367 SVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATK 426

Query: 427 GEQVKLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIA------SSKH 486
           GE V+  GG GMI+ N   +GE   AD HVLPA ++G  S G  I  YI+      SSKH
Sbjct: 427 GEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGA-SGGDEIRRYISESSKSRSSKH 486

Query: 487 QPKASITFEGTKYRSR-APIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTE 546
            P A+I F+GT+   R AP+VA+FS+RGP+   P ++KPD+ APG+NILAAWP  + P+ 
Sbjct: 487 -PTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 546

Query: 547 LGSDKRRVLFNIISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLI 606
           + SD RR  FNI+SGTSM+CPHVSG+AALLK+ H +WSPAAI+SAL+TTAY  DN    +
Sbjct: 547 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 606

Query: 607 SDVGRASGGPANAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRG 666
            D   ++G  ++    GSGHV P KA+DPGL+YDI   DY+N+LC+SNYT   +  ++R 
Sbjct: 607 MD--ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRR 666

Query: 667 NFTCPSKRTLLQPGDLNYPSFSVFMKKKAKNVIVT-LKRTVTNVGSPRSDYTVKINNPTG 726
              C   R     G+LNYPSFSV  ++  ++ + T   RTVTNVG   S Y +KI  P G
Sbjct: 667 QADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRG 726

Query: 727 ITIIVKPEKLSFRRLGQKLSY--QVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVT 769
            T+ V+PEKLSFRR+GQKLS+  +V    +      +    G ++W  GK  V SP+ VT
Sbjct: 727 TTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 772

BLAST of Tan0011054 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.9e-192
Identity = 362/778 (46.53%), Postives = 502/778 (64.52%), Query Frame = 0

Query: 6   IWLLLS---IMLATSTAAVADDQQTYIIHMDPTRMAT--TNPEQWYTSMIDSVNELSSLD 65
           ++++LS   I L   T      ++TY+IHMD + M    TN  QWY+S I+SV +  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 66  EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQF 125
             QEE  N   ILY Y+TA  G +A+L+  +   L +  G VA   +   +LHTT +P F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 126 LGLRR--GHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFS 185
           LGL R     +W       D+++GV+D+GIWPE  SF D G  PVP  W+G CE G++F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 186 RSNCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYN 245
           + NCN+K++GAR + +GYEA  G+++    ++S RD DGHGTHTA+T AG+ V  A+L+ 
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 246 QGMGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYND 305
              G A G++  +R+AAYKVCW  GC S+DIL+A+D+AV DGV VLS+SLGGG  ++  D
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 306 EMAIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQI 365
            ++IATFGA+  GVFVSCSAGN GP   ++ NV+PWI TV AS  DR FPAT+K+G  + 
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 366 FKGSSLYSGNNI----DQLPLVY--NNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNS 425
           FKG SLY G  +     Q PLVY   N +  +  + C  G+L    V GKIV+C++G   
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 426 RLEKGEQVKLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQP 485
           R++KG+ VK AGG GM+L N    GE+  AD H+LPA+ +G +  GK I  Y  +SK + 
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKE-GKLIKQYAMTSK-KA 491

Query: 486 KASITFEGTKYRSR-APIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELG 545
            AS+   GT+   + +P+VAAFSSRGP+F    ++KPD+ APGVNILAAW   ++P+ L 
Sbjct: 492 TASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLS 551

Query: 546 SDKRRVLFNIISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISD 605
           SD RRV FNI+SGTSMSCPHVSG+AAL+KS H +WSPAAIKSALMTTAY+ DN  + ++D
Sbjct: 552 SDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD 611

Query: 606 VGRASGGPANAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSR-GN 665
              A+  P++ +  G+GH+DP +A DPGL+YDI PQ+Y  +LC+ + +  Q+ + ++  N
Sbjct: 612 ASGAA--PSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSN 671

Query: 666 FTCPSKRTLLQ-PGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGI 725
            TC  K TL + PG+LNYP+ S    +      +TL+RTVTNVG   S Y V ++   G 
Sbjct: 672 RTC--KHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGA 731

Query: 726 TIIVKPEKLSFRRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTW 768
           ++ V+P+ L+F    QKLSY V+F    +   +    FG L+W S  + VRSP+ +TW
Sbjct: 732 SVTVQPKTLNFTSKHQKLSYTVTFRTRFR---MKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Tan0011054 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 5.5e-192
Identity = 364/755 (48.21%), Postives = 487/755 (64.50%), Query Frame = 0

Query: 22  ADDQQTYIIHMDPTRMATTNP--EQWYTSMIDSVNELSSLDEDQEEASNAAEILYVYKTA 81
           + DQ TYI+HM  ++M ++      WY S + S+             S++AE+LY Y+ A
Sbjct: 26  SSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-------------SDSAELLYTYENA 85

Query: 82  LSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQFLGL-RRGHGLWNSSNMASD 141
           + GFS +L+  +   L   PG ++   +   +LHTT TP FLGL      L+  +   SD
Sbjct: 86  IHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSD 145

Query: 142 IIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRSNCNKKLIGARAYIKGYEA 201
           +++GV+D+G+WPE  S+ D+GF P+P  WKG CE G  F+ S CN+KLIGAR + +GYE+
Sbjct: 146 VVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYES 205

Query: 202 ILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMGVAAGISFTSRIAAYKV 261
            +G ++ +   RS RD DGHGTHT+STAAG++V  ASL     G A G++  +R+A YKV
Sbjct: 206 TMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKV 265

Query: 262 CWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEMAIATFGAVRNGVFVSCSA 321
           CW  GC S+DILAAID+A+ D V+VLS+SLGGG   +Y D +AI  F A+  G+ VSCSA
Sbjct: 266 CWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSA 325

Query: 322 GNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFKGSSLYSGNNIDQ--LPLV 381
           GN+GP  S++ NVAPWI TV A   DR FPA   LGNG+ F G SL+ G  +    LP +
Sbjct: 326 GNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFI 385

Query: 382 Y-NNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQVKLAGGAGMILINIRL 441
           Y  N +     N+C  G+L+P  VKGKIV+C++G N+R++KG+ VK AGG GMIL N   
Sbjct: 386 YAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAA 445

Query: 442 EGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEGTKYRSR-APIVAAFS 501
            GE+  AD H+LPA T+G + AG  I +Y+ +  + P ASI+  GT    + +P+VAAFS
Sbjct: 446 NGEELVADAHLLPATTVGEK-AGDIIRHYVTTDPN-PTASISILGTVVGVKPSPVVAAFS 505

Query: 502 SRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFNIISGTSMSCPHVSG 561
           SRGP+   P ++KPD+ APGVNILAAW     PT L SD RRV FNIISGTSMSCPHVSG
Sbjct: 506 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 565

Query: 562 IAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPANAFALGSGHVDPEK 621
           +AALLKSVH  WSPAAI+SALMTTAY T    + + D+  A+G P+  F  G+GHV P  
Sbjct: 566 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPSTPFDHGAGHVSPTT 625

Query: 622 AVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTC-PSKRTLLQPGDLNYPSFSVF 681
           A +PGLIYD+  +DYL +LC+ NYTS Q+  VSR N+TC PSK       DLNYPSF+V 
Sbjct: 626 ATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS--YSVADLNYPSFAVN 685

Query: 682 MKKKAKNVIVTLKRTVTNVGSPRSDYTVKI-NNPTGITIIVKPEKLSFRRLGQKLSYQVS 741
           +            RTVT+VG     Y+VK+ +  TG+ I V+P  L+F+   +K SY V+
Sbjct: 686 VDGVG---AYKYTRTVTSVGG-AGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVT 745

Query: 742 FVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTW 768
           F  +   K     SFGS+ W  GK+VV SP+A++W
Sbjct: 746 F-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Tan0011054 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 4.5e-186
Identity = 359/752 (47.74%), Postives = 467/752 (62.10%), Query Frame = 0

Query: 25  QQTYII---HMDPTRMATTNPEQWYTSMIDSVNELSSLDEDQEEASNAAEILYVYKTALS 84
           ++TYII   H D      T+   WYTS ++S + L                LY Y T+  
Sbjct: 27  KKTYIIRVNHSDKPESFLTH-HDWYTSQLNSESSL----------------LYTYTTSFH 86

Query: 85  GFSAKL-STRKLHLLSKIPGFVAATRDELLQLHTTHTPQFLGLRRGHGLWNSSNMASDII 144
           GFSA L ST    LLS     +    D L  LHTT TP+FLGL    G+ +  + ++ +I
Sbjct: 87  GFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVI 146

Query: 145 IGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRSNCNKKLIGARAYIKGYE-AI 204
           IGV+D+G+WPE  SF D   P +P KWKG CE G  F    CNKKLIGAR++ KG++ A 
Sbjct: 147 IGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMAS 206

Query: 205 LGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMGVAAGISFTSRIAAYKVC 264
            G  ++     S RD DGHGTHT++TAAG+ V  AS      G A G++  +R+A YKVC
Sbjct: 207 GGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVC 266

Query: 265 WPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEMAIATFGAVRNGVFVSCSAG 324
           W  GC  +DILAA+DRA++DGVDVLSLSLGGG   +Y D +AI  F A+  GVFVSCSAG
Sbjct: 267 WSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAG 326

Query: 325 NSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFKGSSLYSGNNIDQLP--LVY 384
           NSGP  ++V NVAPW+MTV A   DR FPA   LGNG+   G SLYSG  +   P  LVY
Sbjct: 327 NSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY 386

Query: 385 NNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQVKLAGGAGMILINIRLEG 444
           N       +N+C  GSL  S+V+GKIVVC++G N+R+EKG  V+ AGG GMI+ N    G
Sbjct: 387 NK-GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASG 446

Query: 445 EDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEGTKYRSR-APIVAAFSSR 504
           E+  AD H+LPAI +G +  G  +  Y+ S   +P A + F+GT    + +P+VAAFSSR
Sbjct: 447 EELVADSHLLPAIAVG-KKTGDLLREYVKSDS-KPTALLVFKGTVLDVKPSPVVAAFSSR 506

Query: 505 GPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFNIISGTSMSCPHVSGIA 564
           GP+   P ++KPD+  PGVNILA W   + PT L  D RR  FNI+SGTSMSCPH+SG+A
Sbjct: 507 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 566

Query: 565 ALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPANAFALGSGHVDPEKAV 624
            LLK+ H  WSP+AIKSALMTTAY+ DN    + D   A    +N +A GSGHVDP+KA+
Sbjct: 567 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDA--ADNSLSNPYAHGSGHVDPQKAL 626

Query: 625 DPGLIYDIAPQDYLNYLCSSNYTSKQ-VALVSRGNFTCPSKRTLLQPGDLNYPSFSVFMK 684
            PGL+YDI+ ++Y+ +LCS +YT    VA+V R +  C  K     PG LNYPSFSV   
Sbjct: 627 SPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK--FSDPGQLNYPSFSVLFG 686

Query: 685 KKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSFRRLGQKLSYQVSFVA 744
            K    +V   R VTNVG+  S Y V +N    + I VKP KLSF+ +G+K  Y V+FV+
Sbjct: 687 GKR---VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 746

Query: 745 LGKGKALSEFSFGSLIWLSGKYVVRSPIAVTW 768
                  ++  FGS+ W + ++ VRSP+A +W
Sbjct: 747 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of Tan0011054 vs. NCBI nr
Match: XP_022989707.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022989715.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 652/769 (84.79%), Postives = 703/769 (91.42%), Query Frame = 0

Query: 1   MGIREIW-LLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSL 60
           MGI E+W LLLSIMLATS AAV  DQQTYIIHMD T+MATTNPEQWYTS+I S+N+LSS+
Sbjct: 1   MGITELWLLLLSIMLATSAAAV--DQQTYIIHMDATKMATTNPEQWYTSIIHSINQLSSI 60

Query: 61  DEDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQ 120
           ++DQ EASNAAEILY+YKTA+SGFSAKLSTRKLH LSK+PGF+AAT D+LLQLHTTHTPQ
Sbjct: 61  NDDQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQ 120

Query: 121 FLGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSR 180
           FLGL+RGHGLWN+SN+ASDIIIGVID+GIWPEHISFQDKG PPVPKKWKG C+ G KFSR
Sbjct: 121 FLGLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSR 180

Query: 181 SNCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQ 240
           SNCNKKL+GARAYIKGYE ILGRLN TGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQ
Sbjct: 181 SNCNKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQ 240

Query: 241 GMGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDE 300
           GMG A G+ FTSRIAAYKVCWPEGCAS DILAAIDRAV+DGVDVLSLSLGGGDG FY DE
Sbjct: 241 GMGAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDE 300

Query: 301 MAIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIF 360
           +AIA FGAVRNGVFVSCSAGNSGPF+STV NVAPWIMTVAASYTDRTFP ++KLGNGQIF
Sbjct: 301 IAIAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIF 360

Query: 361 KGSSLYSGNNIDQLPLVYNNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQ 420
           +GSSL+SGN+I +LPLVYN TAGGEEA+VC AGSLVPSMVKGKIVVCE+GTNSR EKGEQ
Sbjct: 361 EGSSLHSGNSIGELPLVYNKTAGGEEASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQ 420

Query: 421 VKLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFE 480
           VKLAGG GMILIN +LEGE+ F D HVLPAI LG  SAGKAIINYIASSKH PKASI FE
Sbjct: 421 VKLAGGVGMILINTQLEGEELFGDSHVLPAINLGA-SAGKAIINYIASSKHPPKASILFE 480

Query: 481 GTKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLF 540
           GT+Y SRAP +AAFSSRGPSFFEPYVIKPDITAPGVNILAAWP +VSP+EL SDKRRVLF
Sbjct: 481 GTRYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLF 540

Query: 541 NIISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGP 600
           NIISGTSMSCPHVSGIAALLKS HKNWSPAAIKSALMTTAY+ DNKR LISDVGR SGGP
Sbjct: 541 NIISGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGP 600

Query: 601 ANAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTL 660
           A+ +A GSGHVDPEKAVDPGL+YDI PQDYLNYLCS NYTSKQV LVSRGNF+CPSKRT+
Sbjct: 601 ADPYAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTV 660

Query: 661 LQPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLS 720
           LQPGDLNYPSFSV MK KAKN  V  KRTVTNVG+P SDYTVKINNP+GI + VKPEKLS
Sbjct: 661 LQPGDLNYPSFSVSMKNKAKN--VRFKRTVTNVGTPSSDYTVKINNPSGIRVSVKPEKLS 720

Query: 721 FRRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           FRR GQKLSYQVSFVALGK + LS+FSFGSL+W+SGKY VRSPIAV W+
Sbjct: 721 FRRSGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Tan0011054 vs. NCBI nr
Match: KAG6599889.1 (Subtilisin-like protease 1.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 642/768 (83.59%), Postives = 699/768 (91.02%), Query Frame = 0

Query: 1   MGIREIWLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLD 60
           MGI E+WLLLSIMLATS AA A DQQ+YIIHMD T+MATTNP+QWYTS+ID++N+LSS++
Sbjct: 1   MGITELWLLLSIMLATSAAAAA-DQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIN 60

Query: 61  EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQF 120
           +DQ EASNAAEILY+YKTA+SGFSAKLSTRKLH LSK+PGF++AT D+LLQLHTTHTP+F
Sbjct: 61  DDQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKF 120

Query: 121 LGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRS 180
           LGL+RGHGLWN+SN+ASDIIIGVID+GIWPEHISFQDKG P VPKKWKG C+ G KFSRS
Sbjct: 121 LGLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRS 180

Query: 181 NCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240
           NCNKKLIGA AYIKGYE ILGRLN TGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG
Sbjct: 181 NCNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240

Query: 241 MGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEM 300
           MG A G+ FTSRIAAYKVCWPEGCAS DILAAIDRAV+DGVDVLSLSLGGGDG FY DE+
Sbjct: 241 MGAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEI 300

Query: 301 AIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFK 360
           AIA FGAVRNGVFVSCSAGNSGPF+STV NVAPWIMTVAASYTDRTF  ++KLGNGQIF+
Sbjct: 301 AIAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFE 360

Query: 361 GSSLYSGNNIDQLPLVYNNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQV 420
           GSSL+SGN+I QLPLVYN TAGGEEANVC AGSLVPSMVKGKIVVCE+GTNSR EKGEQV
Sbjct: 361 GSSLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEG 480
           KLAGG GMILIN +LEGE+ FAD HVLPA+ LG  SAGKAIINYIASSK  PKASI FEG
Sbjct: 421 KLAGGVGMILINTQLEGEELFADSHVLPAVNLGA-SAGKAIINYIASSKQPPKASILFEG 480

Query: 481 TKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFN 540
           T+Y SRAP +AAFSSRGPSFFEPYVIKPDITAPGVNILAAWP +VSP+EL SDKRRVLFN
Sbjct: 481 TRYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFN 540

Query: 541 IISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPA 600
           IISGTSMSCPHVSGIAALLKS H NWSPAAIKSALMTTAY+ DNKR +ISDVGR SGGPA
Sbjct: 541 IISGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPA 600

Query: 601 NAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTLL 660
           + +A GSGHVDPEKAVDPGL+YDI PQDYLNYLCS NYTSKQV LVSRGNF+CPSKR L+
Sbjct: 601 DPYAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRNLV 660

Query: 661 QPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSF 720
           QPGDLNYPSFSV MK +AKN  VT KRTVTNVG+P SDYTVKINNP+GI + VKP+KLSF
Sbjct: 661 QPGDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSF 720

Query: 721 RRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           RR GQKL YQVSFVALGK + LS+FSFGSL+W+SGKY VRSPIAV W+
Sbjct: 721 RRSGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Tan0011054 vs. NCBI nr
Match: XP_022943077.1 (subtilisin-like protease SBT1.1 [Cucurbita moschata] >XP_022943079.1 subtilisin-like protease SBT1.1 [Cucurbita moschata])

HSP 1 Score: 1290.0 bits (3337), Expect = 0.0e+00
Identity = 641/768 (83.46%), Postives = 698/768 (90.89%), Query Frame = 0

Query: 1   MGIREIWLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLD 60
           MGI E+WLLLSIMLATS AA A DQQ+YIIHMD T+MATTNP+QWYTS+ID++N+LSS++
Sbjct: 1   MGITELWLLLSIMLATSAAAAA-DQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIN 60

Query: 61  EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQF 120
           +DQ EASNAAEILY+YKTA+SGFSAKLSTRKLH LSK+PGF++AT D+LLQLHTTHTP+F
Sbjct: 61  DDQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKF 120

Query: 121 LGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRS 180
           LGL+RGHGLWN+SN+ASDIIIGVID+GIWPEHISFQDKG P VPKKWKG C+ G KFSRS
Sbjct: 121 LGLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRS 180

Query: 181 NCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240
           NCNKKLIGA AYIKGYE ILGRLN TGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG
Sbjct: 181 NCNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240

Query: 241 MGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEM 300
           MG A G+ FTSRIAAYKVCWPEGCAS DILAAIDRAV+DGVDVLSLSLGGGDG FY DE+
Sbjct: 241 MGAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEI 300

Query: 301 AIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFK 360
           AIA FGAVRNGVFVSCSAGNSGPF+STV NVAPWIMTVAASYTDRTF  ++KLGNGQIF+
Sbjct: 301 AIAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFE 360

Query: 361 GSSLYSGNNIDQLPLVYNNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQV 420
           GSSL+SGN+I QLPLVYN TAGGEEANVC AGSLVPSMVKGKIVVCE+GTNSR EKGEQV
Sbjct: 361 GSSLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEG 480
           KLAGG GMILIN +LEGE+ FAD HVLPA+ LG  SAGKAIINYIASSK  PKASI FEG
Sbjct: 421 KLAGGVGMILINTQLEGEELFADSHVLPAVNLGA-SAGKAIINYIASSKQPPKASILFEG 480

Query: 481 TKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFN 540
           T+Y SRAP +AAFSSRGPSFFEPYVIKPDITAPGVNILAAWP +VSP+EL SDKRRVLFN
Sbjct: 481 TRYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFN 540

Query: 541 IISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPA 600
           IISGTSMSCPHVSGIAALLKS H NWSPAAIKSALMTTAY+ DNKR +ISDVGR SGGPA
Sbjct: 541 IISGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPA 600

Query: 601 NAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTLL 660
           + +A GSGHVDPEKAVDPGL+YDI PQDYLNYLCS NYTSKQV LVSRGNF+CPS R L+
Sbjct: 601 DPYAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSNRNLV 660

Query: 661 QPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSF 720
           QPGDLNYPSFSV MK +AKN  VT KRTVTNVG+P SDYTVKINNP+GI + VKP+KLSF
Sbjct: 661 QPGDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSF 720

Query: 721 RRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           RR GQKL YQVSFVALGK + LS+FSFGSL+W+SGKY VRSPIAV W+
Sbjct: 721 RRSGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Tan0011054 vs. NCBI nr
Match: XP_023544752.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1288.1 bits (3332), Expect = 0.0e+00
Identity = 643/768 (83.72%), Postives = 700/768 (91.15%), Query Frame = 0

Query: 1   MGIREIWLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLD 60
           MGI E+WLLLSIMLATS AAV  DQQ+YIIHMD T+MATTNP+QWYTS+ID+VN+LSS++
Sbjct: 1   MGITELWLLLSIMLATSAAAV--DQQSYIIHMDTTKMATTNPQQWYTSIIDTVNQLSSIN 60

Query: 61  EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQF 120
           +DQ EASNAAEILY+YKTA+SGFSAKLSTRKL  LSK+PGF++AT D+LLQLHTTHTP+F
Sbjct: 61  DDQNEASNAAEILYIYKTAISGFSAKLSTRKLLSLSKLPGFLSATPDKLLQLHTTHTPKF 120

Query: 121 LGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRS 180
           LGL+RGHGLWN+S +ASDIIIGVID+GIWPEHISFQDKG  PVPKKWKG C+ G KFSRS
Sbjct: 121 LGLQRGHGLWNASYLASDIIIGVIDTGIWPEHISFQDKGLSPVPKKWKGTCQAGPKFSRS 180

Query: 181 NCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240
           NCN KLIGARAYIKGYE ILGRLN TGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG
Sbjct: 181 NCNNKLIGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240

Query: 241 MGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEM 300
           MG A G+ FTSRIAAYKVCWPEGCAS DILAAIDRAV+DGVDVLSLSLGGGDG FY DE+
Sbjct: 241 MGAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEI 300

Query: 301 AIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFK 360
           AIA FGAVRNGVFVSCSAGNSGPF+STV NVAPWIMTVAASYTDRTF  ++KLGNGQIF+
Sbjct: 301 AIAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFE 360

Query: 361 GSSLYSGNNIDQLPLVYNNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQV 420
           GSSL+SGN+I QLPLVYN TAGGEEANVC AGSLVPSMVKGKIVVCE+GTNSR EKGEQV
Sbjct: 361 GSSLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEG 480
           KLAGG GMILIN +LEGE+ FAD HVLPA+ LG  SAGKAI NYIASSK  PKASI FEG
Sbjct: 421 KLAGGVGMILINTQLEGEELFADSHVLPAVNLGA-SAGKAITNYIASSKQPPKASILFEG 480

Query: 481 TKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFN 540
           T+Y SRAP +AAFSSRGPSFFEPYVIKPDITAPGVNILAAWP +VSP+EL SD+RRVLFN
Sbjct: 481 TRYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDERRVLFN 540

Query: 541 IISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPA 600
           IISGTSMSCPHVSGIAALLKS HKNWSPAAIKSALMTTAY+TDNKR +ISDVGR SGGPA
Sbjct: 541 IISGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVTDNKRSVISDVGRPSGGPA 600

Query: 601 NAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTLL 660
           + +A GSGHVDPEKAVDPGL+YDIAPQDYLNYLCS NYTSKQV LVSRGNF+CPSK T+L
Sbjct: 601 DPYAFGSGHVDPEKAVDPGLVYDIAPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKGTVL 660

Query: 661 QPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSF 720
           QPGDLNYPSFSV MK +AKN  VTLKRTVTNVG+P SDYTVKINNP+GI + VKP+KLSF
Sbjct: 661 QPGDLNYPSFSVSMKNRAKN--VTLKRTVTNVGTPTSDYTVKINNPSGIAVSVKPKKLSF 720

Query: 721 RRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           RR GQKLSYQVSFVA GK + LS+FSFGSL+W+SGKY VRSPIAV W+
Sbjct: 721 RRSGQKLSYQVSFVASGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 763

BLAST of Tan0011054 vs. NCBI nr
Match: XP_022941552.1 (subtilisin-like protease SBT1.1 [Cucurbita moschata] >XP_022941553.1 subtilisin-like protease SBT1.1 [Cucurbita moschata])

HSP 1 Score: 1235.3 bits (3195), Expect = 0.0e+00
Identity = 622/768 (80.99%), Postives = 679/768 (88.41%), Query Frame = 0

Query: 1   MGIREIWLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLD 60
           MG RE+ L LSI LATSTAAV  DQQ+YIIHMD T+MA   PEQWYT++IDS+NE+SSL 
Sbjct: 1   MGFREVCLFLSIFLATSTAAV--DQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSL- 60

Query: 61  EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQF 120
           EDQEEASNAA+ILYVYKTA+SGF+AKLST+KLH LSK PGF+AAT +ELLQLHTTH+PQF
Sbjct: 61  EDQEEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRS 180
           LGL+R HGLWNSSN+ASDI+IG++D+GIWPEHISFQDKG PPVPKKWKG C+ GQKFS S
Sbjct: 121 LGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPS 180

Query: 181 NCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240
           NCN+KLIGA AYIKGYEAI+GRLNATGTFRS RDSDGHGTHTASTAAGNIV KAS +NQ 
Sbjct: 181 NCNRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQA 240

Query: 241 MGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEM 300
           MGVA G+ FTSRIAAYKVCW EGCA+ADILAAIDRAV DGVDVLSLSLGG   +FY D++
Sbjct: 241 MGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDI 300

Query: 301 AIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFK 360
           AIATFGAVRNGVFVSCSAGNSGP  STV N+APWIMTVAASYTDR+FPAT+KLGNGQ+F+
Sbjct: 301 AIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFE 360

Query: 361 GSSLYSGNNIDQLPLVYNNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQV 420
           GSSLYSGNNI QLPLVYNNTAGGE+ANVC AGSLVPSMVKGKIVVCE+GTNSR  KGEQV
Sbjct: 361 GSSLYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEG 480
           KLAGGAGMILIN +LEGE+ FADPHVLPA  LG  SAG+AII YI+SSKHQPKA I FEG
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGA-SAGQAIIRYISSSKHQPKALIAFEG 480

Query: 481 TKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFN 540
           TK+ +RAP VAAFSSRGPS   P VIKPD+TAPGVNILAAWP I SP+E+ SDKRRVLFN
Sbjct: 481 TKFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFN 540

Query: 541 IISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPA 600
           +ISGTSMSCPHVSG+AALLKS HK+WSPAAIKSALMTTAY  DN+   ISDVG ASG PA
Sbjct: 541 VISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPA 600

Query: 601 NAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTLL 660
           N FA GSGHVDPEKA DPGLIYDI PQDYLNYLCS NY S Q+ LVSRGNFTCPSKR + 
Sbjct: 601 NPFAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVG 660

Query: 661 QPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSF 720
           Q G LNYPSFSVFMKKKAKNV VTLKRTVTNVG PRSDY+VKI NP GI I VKPEKLSF
Sbjct: 661 QAGKLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSF 720

Query: 721 RRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           RR GQKLSYQVSFVALGK + +S FSFGSL+W+SG Y VRSPIAVTW+
Sbjct: 721 RRYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Tan0011054 vs. ExPASy TrEMBL
Match: A0A6J1JN56 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111486706 PE=3 SV=1)

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 652/769 (84.79%), Postives = 703/769 (91.42%), Query Frame = 0

Query: 1   MGIREIW-LLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSL 60
           MGI E+W LLLSIMLATS AAV  DQQTYIIHMD T+MATTNPEQWYTS+I S+N+LSS+
Sbjct: 1   MGITELWLLLLSIMLATSAAAV--DQQTYIIHMDATKMATTNPEQWYTSIIHSINQLSSI 60

Query: 61  DEDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQ 120
           ++DQ EASNAAEILY+YKTA+SGFSAKLSTRKLH LSK+PGF+AAT D+LLQLHTTHTPQ
Sbjct: 61  NDDQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQ 120

Query: 121 FLGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSR 180
           FLGL+RGHGLWN+SN+ASDIIIGVID+GIWPEHISFQDKG PPVPKKWKG C+ G KFSR
Sbjct: 121 FLGLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSR 180

Query: 181 SNCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQ 240
           SNCNKKL+GARAYIKGYE ILGRLN TGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQ
Sbjct: 181 SNCNKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQ 240

Query: 241 GMGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDE 300
           GMG A G+ FTSRIAAYKVCWPEGCAS DILAAIDRAV+DGVDVLSLSLGGGDG FY DE
Sbjct: 241 GMGAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDE 300

Query: 301 MAIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIF 360
           +AIA FGAVRNGVFVSCSAGNSGPF+STV NVAPWIMTVAASYTDRTFP ++KLGNGQIF
Sbjct: 301 IAIAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIF 360

Query: 361 KGSSLYSGNNIDQLPLVYNNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQ 420
           +GSSL+SGN+I +LPLVYN TAGGEEA+VC AGSLVPSMVKGKIVVCE+GTNSR EKGEQ
Sbjct: 361 EGSSLHSGNSIGELPLVYNKTAGGEEASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQ 420

Query: 421 VKLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFE 480
           VKLAGG GMILIN +LEGE+ F D HVLPAI LG  SAGKAIINYIASSKH PKASI FE
Sbjct: 421 VKLAGGVGMILINTQLEGEELFGDSHVLPAINLGA-SAGKAIINYIASSKHPPKASILFE 480

Query: 481 GTKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLF 540
           GT+Y SRAP +AAFSSRGPSFFEPYVIKPDITAPGVNILAAWP +VSP+EL SDKRRVLF
Sbjct: 481 GTRYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLF 540

Query: 541 NIISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGP 600
           NIISGTSMSCPHVSGIAALLKS HKNWSPAAIKSALMTTAY+ DNKR LISDVGR SGGP
Sbjct: 541 NIISGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGP 600

Query: 601 ANAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTL 660
           A+ +A GSGHVDPEKAVDPGL+YDI PQDYLNYLCS NYTSKQV LVSRGNF+CPSKRT+
Sbjct: 601 ADPYAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTV 660

Query: 661 LQPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLS 720
           LQPGDLNYPSFSV MK KAKN  V  KRTVTNVG+P SDYTVKINNP+GI + VKPEKLS
Sbjct: 661 LQPGDLNYPSFSVSMKNKAKN--VRFKRTVTNVGTPSSDYTVKINNPSGIRVSVKPEKLS 720

Query: 721 FRRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           FRR GQKLSYQVSFVALGK + LS+FSFGSL+W+SGKY VRSPIAV W+
Sbjct: 721 FRRSGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Tan0011054 vs. ExPASy TrEMBL
Match: A0A6J1FT86 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111447921 PE=3 SV=1)

HSP 1 Score: 1290.0 bits (3337), Expect = 0.0e+00
Identity = 641/768 (83.46%), Postives = 698/768 (90.89%), Query Frame = 0

Query: 1   MGIREIWLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLD 60
           MGI E+WLLLSIMLATS AA A DQQ+YIIHMD T+MATTNP+QWYTS+ID++N+LSS++
Sbjct: 1   MGITELWLLLSIMLATSAAAAA-DQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIN 60

Query: 61  EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQF 120
           +DQ EASNAAEILY+YKTA+SGFSAKLSTRKLH LSK+PGF++AT D+LLQLHTTHTP+F
Sbjct: 61  DDQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKF 120

Query: 121 LGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRS 180
           LGL+RGHGLWN+SN+ASDIIIGVID+GIWPEHISFQDKG P VPKKWKG C+ G KFSRS
Sbjct: 121 LGLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRS 180

Query: 181 NCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240
           NCNKKLIGA AYIKGYE ILGRLN TGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG
Sbjct: 181 NCNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240

Query: 241 MGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEM 300
           MG A G+ FTSRIAAYKVCWPEGCAS DILAAIDRAV+DGVDVLSLSLGGGDG FY DE+
Sbjct: 241 MGAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEI 300

Query: 301 AIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFK 360
           AIA FGAVRNGVFVSCSAGNSGPF+STV NVAPWIMTVAASYTDRTF  ++KLGNGQIF+
Sbjct: 301 AIAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFE 360

Query: 361 GSSLYSGNNIDQLPLVYNNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQV 420
           GSSL+SGN+I QLPLVYN TAGGEEANVC AGSLVPSMVKGKIVVCE+GTNSR EKGEQV
Sbjct: 361 GSSLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQV 420

Query: 421 KLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEG 480
           KLAGG GMILIN +LEGE+ FAD HVLPA+ LG  SAGKAIINYIASSK  PKASI FEG
Sbjct: 421 KLAGGVGMILINTQLEGEELFADSHVLPAVNLGA-SAGKAIINYIASSKQPPKASILFEG 480

Query: 481 TKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFN 540
           T+Y SRAP +AAFSSRGPSFFEPYVIKPDITAPGVNILAAWP +VSP+EL SDKRRVLFN
Sbjct: 481 TRYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFN 540

Query: 541 IISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPA 600
           IISGTSMSCPHVSGIAALLKS H NWSPAAIKSALMTTAY+ DNKR +ISDVGR SGGPA
Sbjct: 541 IISGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPA 600

Query: 601 NAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTLL 660
           + +A GSGHVDPEKAVDPGL+YDI PQDYLNYLCS NYTSKQV LVSRGNF+CPS R L+
Sbjct: 601 DPYAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSNRNLV 660

Query: 661 QPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSF 720
           QPGDLNYPSFSV MK +AKN  VT KRTVTNVG+P SDYTVKINNP+GI + VKP+KLSF
Sbjct: 661 QPGDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSF 720

Query: 721 RRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           RR GQKL YQVSFVALGK + LS+FSFGSL+W+SGKY VRSPIAV W+
Sbjct: 721 RRSGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Tan0011054 vs. ExPASy TrEMBL
Match: A0A6J1FMR4 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111446877 PE=3 SV=1)

HSP 1 Score: 1235.3 bits (3195), Expect = 0.0e+00
Identity = 622/768 (80.99%), Postives = 679/768 (88.41%), Query Frame = 0

Query: 1   MGIREIWLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLD 60
           MG RE+ L LSI LATSTAAV  DQQ+YIIHMD T+MA   PEQWYT++IDS+NE+SSL 
Sbjct: 1   MGFREVCLFLSIFLATSTAAV--DQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSL- 60

Query: 61  EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQF 120
           EDQEEASNAA+ILYVYKTA+SGF+AKLST+KLH LSK PGF+AAT +ELLQLHTTH+PQF
Sbjct: 61  EDQEEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRS 180
           LGL+R HGLWNSSN+ASDI+IG++D+GIWPEHISFQDKG PPVPKKWKG C+ GQKFS S
Sbjct: 121 LGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPS 180

Query: 181 NCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240
           NCN+KLIGA AYIKGYEAI+GRLNATGTFRS RDSDGHGTHTASTAAGNIV KAS +NQ 
Sbjct: 181 NCNRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQA 240

Query: 241 MGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEM 300
           MGVA G+ FTSRIAAYKVCW EGCA+ADILAAIDRAV DGVDVLSLSLGG   +FY D++
Sbjct: 241 MGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDI 300

Query: 301 AIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFK 360
           AIATFGAVRNGVFVSCSAGNSGP  STV N+APWIMTVAASYTDR+FPAT+KLGNGQ+F+
Sbjct: 301 AIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFE 360

Query: 361 GSSLYSGNNIDQLPLVYNNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQV 420
           GSSLYSGNNI QLPLVYNNTAGGE+ANVC AGSLVPSMVKGKIVVCE+GTNSR  KGEQV
Sbjct: 361 GSSLYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEG 480
           KLAGGAGMILIN +LEGE+ FADPHVLPA  LG  SAG+AII YI+SSKHQPKA I FEG
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGA-SAGQAIIRYISSSKHQPKALIAFEG 480

Query: 481 TKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFN 540
           TK+ +RAP VAAFSSRGPS   P VIKPD+TAPGVNILAAWP I SP+E+ SDKRRVLFN
Sbjct: 481 TKFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFN 540

Query: 541 IISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPA 600
           +ISGTSMSCPHVSG+AALLKS HK+WSPAAIKSALMTTAY  DN+   ISDVG ASG PA
Sbjct: 541 VISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPA 600

Query: 601 NAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTLL 660
           N FA GSGHVDPEKA DPGLIYDI PQDYLNYLCS NY S Q+ LVSRGNFTCPSKR + 
Sbjct: 601 NPFAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVG 660

Query: 661 QPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSF 720
           Q G LNYPSFSVFMKKKAKNV VTLKRTVTNVG PRSDY+VKI NP GI I VKPEKLSF
Sbjct: 661 QAGKLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSF 720

Query: 721 RRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           RR GQKLSYQVSFVALGK + +S FSFGSL+W+SG Y VRSPIAVTW+
Sbjct: 721 RRYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Tan0011054 vs. ExPASy TrEMBL
Match: A0A6J1JPC3 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111487676 PE=3 SV=1)

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 621/768 (80.86%), Postives = 679/768 (88.41%), Query Frame = 0

Query: 1   MGIREIWLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLD 60
           MG RE+ L LSI LATS AAV  DQQ+YIIHMD T+MA  +PEQWYT++IDS+N++SSLD
Sbjct: 1   MGFREVCLFLSIFLATSAAAV--DQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLD 60

Query: 61  EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQF 120
            DQEEAS+AA+ILYVYKTA+SGF+AKLST+KLH LSK PGF+AAT +ELLQLHTTH+PQF
Sbjct: 61  -DQEEASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRS 180
           LGL+R HGLWNSSN+ASDI+IG++D+GIWPEHISFQDKG PPVPKKWKG C+ GQKFS S
Sbjct: 121 LGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPS 180

Query: 181 NCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQG 240
           NCN+KLIGA AYIKGYEAI+GRLNATGTFRS RDSDGHGTHTASTAAGNIV KAS +NQ 
Sbjct: 181 NCNRKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQA 240

Query: 241 MGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEM 300
           MGVA G+ FTSRIAAYKVCW EGCA+ADILAAIDRAV DGVDVLSLSLGG   +FY D++
Sbjct: 241 MGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDI 300

Query: 301 AIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFK 360
           AIATFGAVRNGVFVSCSAGNSGP  STV N+APWIMTVAASYTDRTFPAT+KLGNGQ+F+
Sbjct: 301 AIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFE 360

Query: 361 GSSLYSGNNIDQLPLVYNNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQV 420
           GSSLYSGN+I QLPLVYNNTAGGEEANVC AGSLVPS+VKGKIVVCE+GTNSR  KGEQV
Sbjct: 361 GSSLYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEG 480
           KLAGGAGMILIN +LEGE+ FADPHVLPA  LG  SAG+AII YI+SSKHQPKA I FEG
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGA-SAGQAIIRYISSSKHQPKALIAFEG 480

Query: 481 TKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFN 540
           TK+ +RAP VAAFSSRGPS   P VIKPD+TAPGVNILAAWP I SP+EL SDKRRVLFN
Sbjct: 481 TKFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFN 540

Query: 541 IISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPA 600
           IISGTSMSCPHVSG+AALLKS HK+WSPAAIKSALMTTAY  DN+   ISDVG  SG PA
Sbjct: 541 IISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPA 600

Query: 601 NAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTLL 660
           N FA GSGHVDPEKA DPGLIYDI PQDYLNY CS NY S Q+ LVSRGNFTCPSKR + 
Sbjct: 601 NPFAFGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVG 660

Query: 661 QPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSF 720
           QPG+LNYPSFSVFMKKKAKNV VTLKRTVTNVG PRSDYTVKI NP GI I V+PEKLSF
Sbjct: 661 QPGNLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSF 720

Query: 721 RRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           RR GQKLSYQVSFVALGK + L  FSFGSL+W+SGKY VRSPIAVTW+
Sbjct: 721 RRYGQKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of Tan0011054 vs. ExPASy TrEMBL
Match: A0A1S3BZC2 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495085 PE=3 SV=1)

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 597/771 (77.43%), Postives = 668/771 (86.64%), Query Frame = 0

Query: 1   MGIREIWLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLD 60
           MG +E+ L L I + T T+ VA DQQ+YI+HMD T+MATTNPEQWYT++I SVN+LSSLD
Sbjct: 1   MGFKEVLLFLYITMLT-TSIVAMDQQSYIVHMDTTKMATTNPEQWYTAIIHSVNKLSSLD 60

Query: 61  --EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTP 120
              ++E+A N AEILYVYKT +SGF AKLS++ LH LSK+PGF+AA+ +ELLQLHTTH+P
Sbjct: 61  ANNNEEQALNIAEILYVYKTVISGFCAKLSSKNLHFLSKVPGFIAASPNELLQLHTTHSP 120

Query: 121 QFLGLRRGHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFS 180
           +FLGL+RGHGLWNSSN+ASDIIIGV+D+GIWPEHISFQDK  PPVP KWKG+C+ G  FS
Sbjct: 121 KFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKALPPVPSKWKGICQTGPSFS 180

Query: 181 RSNCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYN 240
            SNCNKKLIGAR +I+ YEA +GRLNATGTFRSARDSDGHGTHTASTAAGN V +AS YN
Sbjct: 181 LSNCNKKLIGARTFIQAYEAAVGRLNATGTFRSARDSDGHGTHTASTAAGNFVNRASFYN 240

Query: 241 QGMGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYND 300
           QGMGVA G+ FTSRIAAYKVCWPEGCASADILAA+D AV DGVDVLS+SLGGG    Y+D
Sbjct: 241 QGMGVATGMRFTSRIAAYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIYSD 300

Query: 301 EMAIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQI 360
           ++AIA FGA++ GVFVSCSAGNSGPFISTV NVAPW+MTVAASYTDRTFP T++LGNG +
Sbjct: 301 QIAIAAFGAIQKGVFVSCSAGNSGPFISTVGNVAPWVMTVAASYTDRTFPTTVRLGNGMV 360

Query: 361 FKGSSLYSGNNIDQLPLVYNNTAG-GEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKG 420
           F+GSSLY G N+ + PLVYNNTAG G E N C  GSL P+MVKGKI VCE+GTNSR EKG
Sbjct: 361 FEGSSLYFGKNLKETPLVYNNTAGDGRETNFCTPGSLDPTMVKGKIAVCERGTNSRTEKG 420

Query: 421 EQVKLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASIT 480
           EQVKLAGGAGMILIN  LEGED  AD HVLPA ++G  SAGK+I+NYIASSK Q KASI 
Sbjct: 421 EQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGV-SAGKSILNYIASSKRQAKASIM 480

Query: 481 FEGTKYRSRAPIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRV 540
           F+GTKY SRAP VAAFSSRGPSF++PYVIKPDITAPGVNILAAWP IVSP+EL SDKRRV
Sbjct: 481 FKGTKYGSRAPRVAAFSSRGPSFYKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRV 540

Query: 541 LFNIISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASG 600
           LFNIISGTSMSCPHVSG+AALLKSVHK+WSPAAIKSALMTTAY+TDNK  LISDVGRASG
Sbjct: 541 LFNIISGTSMSCPHVSGLAALLKSVHKDWSPAAIKSALMTTAYVTDNKNHLISDVGRASG 600

Query: 601 GPANAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKR 660
           GPA+ FA GSGHVDPEKA DPGL+YDIAPQDY+ YLCS  Y S Q+ALVSRG FTC SKR
Sbjct: 601 GPADPFAFGSGHVDPEKASDPGLVYDIAPQDYITYLCSLKYNSTQIALVSRGKFTCSSKR 660

Query: 661 TLLQPGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEK 720
           T  QPGDLNYPSFSVFM KK KNV  T KRTVTNVG PRSDYTV+I NP GI IIVKPEK
Sbjct: 661 TFFQPGDLNYPSFSVFM-KKGKNVNSTFKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPEK 720

Query: 721 LSFRRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
           LSF +LG+KLSY+VSFVALGK K+L +FSFGSL+W SG YVVRSPIAVTWQ
Sbjct: 721 LSFVKLGEKLSYKVSFVALGKRKSLDDFSFGSLVWHSGTYVVRSPIAVTWQ 768

BLAST of Tan0011054 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 802.0 bits (2070), Expect = 4.3e-232
Identity = 411/750 (54.80%), Postives = 534/750 (71.20%), Query Frame = 0

Query: 21  VADDQQTYIIHMDPTRMATTNPEQWYTSMIDSVNELSSLDEDQEEASNAAEILYVYKTAL 80
           V+  +QTY+IH       TT+ +   TS+ +S+   +  D+D     +  EI Y+Y+ A+
Sbjct: 37  VSSRKQTYVIH-----TVTTSTKHIVTSLFNSLQTENINDDD----FSLPEIHYIYENAM 96

Query: 81  SGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQFLGLRRGHGLWNSSNMASDII 140
           SGFSA L+  +L  +    GF++A  DELL LHTT++ +FLGL  G GLWN ++++SD+I
Sbjct: 97  SGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSDVI 156

Query: 141 IGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRSNCNKKLIGARAYIKGYEAIL 200
           IG++D+GI PEH+SF+D    PVP +W+G C+ G  FS S CNKK+IGA A+ KGYE+I+
Sbjct: 157 IGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYESIV 216

Query: 201 GRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMGVAAGISFTSRIAAYKVCW 260
           G++N T  FRS RD+ GHGTHTASTAAG+IV KA+ + Q  G+A+G+ FTSRIAAYK CW
Sbjct: 217 GKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKACW 276

Query: 261 PEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEMAIATFGAVRNGVFVSCSAGN 320
             GCAS D++AAIDRA++DGVDV+SLSLGG    FY D +AIA FGA++  +FVSCSAGN
Sbjct: 277 ALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGN 336

Query: 321 SGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFKGSSLYSGNNIDQLPLVYNNT 380
           SGP  STV N APW+MTVAASYTDRTFPA +++GN +   GSSLY G ++  LPL +N T
Sbjct: 337 SGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGKSLKNLPLAFNRT 396

Query: 381 AGGEEANV-CAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQVKLAGGAGMILINIRLEGED 440
           AG E   V C   SL   +V+GKIV+C +G + R  KGE+VK +GGA M+L++   EGE+
Sbjct: 397 AGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSGGAAMLLVSTEAEGEE 456

Query: 441 FFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEGTKYRSRAPIVAAFSSRGPS 500
             ADPHVLPA++LG  S GK ++NY+A + +   AS+ F GT Y + AP+VAAFSSRGPS
Sbjct: 457 LLADPHVLPAVSLGF-SDGKTLLNYLAGAAN-ATASVRFRGTAYGATAPMVAAFSSRGPS 516

Query: 501 FFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFNIISGTSMSCPHVSGIAALL 560
              P + KPDI APG+NILA W    SP+ L SD RRV FNIISGTSM+CPH+SGIAAL+
Sbjct: 517 VAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHISGIAALI 576

Query: 561 KSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASG-GPANAFALGSGHVDPEKAVDP 620
           KSVH +WSPA IKSA+MTTA ITDN+ R I D G A     A AFA G+G+VDP +AVDP
Sbjct: 577 KSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDP 636

Query: 621 GLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTCPSKRTLLQPGDLNYPSFSVFMKKKA 680
           GL+YD +  DYLNYLCS NYTS+++ L S  N+TC S   +L PGDLNYPSF+V +   A
Sbjct: 637 GLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFAVNLVNGA 696

Query: 681 KNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSFRRLGQKLSYQVSFVALGK 740
               V  KRTVTNVGSP  +Y V +  P G+ + V+P+ L F++  ++LSY V++ A   
Sbjct: 697 NLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTVTYDAEAS 756

Query: 741 GKALSEFSFGSLIWLSGKYVVRSPIAVTWQ 769
             + S  SFG L+W+  KY VRSPIAVTW+
Sbjct: 757 RNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Tan0011054 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 676.8 bits (1745), Expect = 2.1e-194
Identity = 371/782 (47.44%), Postives = 493/782 (63.04%), Query Frame = 0

Query: 7   WLLLSIMLATSTAAVADDQQTYIIHMDPTRMATTNPE--QWYTSMIDSVNELSSLDEDQE 66
           +  L  + + S++A + +  TYI+H+D     +  P    WYTS + S+           
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----------- 66

Query: 67  EASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQFLGLR 126
             S+   I++ Y T   GFSA+L+++    L   P  ++   +++  LHTT +P+FLGLR
Sbjct: 67  -TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLR 126

Query: 127 RGH--GLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRSNC 186
                GL   S+  SD++IGVID+G+WPE  SF D+G  PVP KWKG C   Q F  S C
Sbjct: 127 STDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESAC 186

Query: 187 NKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMG 246
           N+KL+GAR +  GYEA  G++N T  FRS RDSDGHGTHTAS +AG  V+ AS      G
Sbjct: 187 NRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHG 246

Query: 247 VAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEMAI 306
           VAAG++  +R+AAYKVCW  GC  +DILAA D AV DGVDV+SLS+GG    +Y D +AI
Sbjct: 247 VAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAI 306

Query: 307 ATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFKGS 366
             FGA+  G+FVS SAGN GP   TV NVAPW+ TV A   DR FPA +KLGNG++  G 
Sbjct: 307 GAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGV 366

Query: 367 SLYSGNNID---QLPLVYNNT---AGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEK 426
           S+Y G  +D     PLVY  +     G  +++C  GSL P++VKGKIV+C++G NSR  K
Sbjct: 367 SVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATK 426

Query: 427 GEQVKLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIA------SSKH 486
           GE V+  GG GMI+ N   +GE   AD HVLPA ++G  S G  I  YI+      SSKH
Sbjct: 427 GEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGA-SGGDEIRRYISESSKSRSSKH 486

Query: 487 QPKASITFEGTKYRSR-APIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTE 546
            P A+I F+GT+   R AP+VA+FS+RGP+   P ++KPD+ APG+NILAAWP  + P+ 
Sbjct: 487 -PTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 546

Query: 547 LGSDKRRVLFNIISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLI 606
           + SD RR  FNI+SGTSM+CPHVSG+AALLK+ H +WSPAAI+SAL+TTAY  DN    +
Sbjct: 547 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 606

Query: 607 SDVGRASGGPANAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRG 666
            D   ++G  ++    GSGHV P KA+DPGL+YDI   DY+N+LC+SNYT   +  ++R 
Sbjct: 607 MD--ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRR 666

Query: 667 NFTCPSKRTLLQPGDLNYPSFSVFMKKKAKNVIVT-LKRTVTNVGSPRSDYTVKINNPTG 726
              C   R     G+LNYPSFSV  ++  ++ + T   RTVTNVG   S Y +KI  P G
Sbjct: 667 QADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRG 726

Query: 727 ITIIVKPEKLSFRRLGQKLSY--QVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVT 769
            T+ V+PEKLSFRR+GQKLS+  +V    +      +    G ++W  GK  V SP+ VT
Sbjct: 727 TTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 772

BLAST of Tan0011054 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 674.1 bits (1738), Expect = 1.3e-193
Identity = 362/778 (46.53%), Postives = 502/778 (64.52%), Query Frame = 0

Query: 6   IWLLLS---IMLATSTAAVADDQQTYIIHMDPTRMAT--TNPEQWYTSMIDSVNELSSLD 65
           ++++LS   I L   T      ++TY+IHMD + M    TN  QWY+S I+SV +  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 66  EDQEEASNAAEILYVYKTALSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQF 125
             QEE  N   ILY Y+TA  G +A+L+  +   L +  G VA   +   +LHTT +P F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 126 LGLRR--GHGLWNSSNMASDIIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFS 185
           LGL R     +W       D+++GV+D+GIWPE  SF D G  PVP  W+G CE G++F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 186 RSNCNKKLIGARAYIKGYEAILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYN 245
           + NCN+K++GAR + +GYEA  G+++    ++S RD DGHGTHTA+T AG+ V  A+L+ 
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 246 QGMGVAAGISFTSRIAAYKVCWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYND 305
              G A G++  +R+AAYKVCW  GC S+DIL+A+D+AV DGV VLS+SLGGG  ++  D
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 306 EMAIATFGAVRNGVFVSCSAGNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQI 365
            ++IATFGA+  GVFVSCSAGN GP   ++ NV+PWI TV AS  DR FPAT+K+G  + 
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 366 FKGSSLYSGNNI----DQLPLVY--NNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNS 425
           FKG SLY G  +     Q PLVY   N +  +  + C  G+L    V GKIV+C++G   
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 426 RLEKGEQVKLAGGAGMILINIRLEGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQP 485
           R++KG+ VK AGG GM+L N    GE+  AD H+LPA+ +G +  GK I  Y  +SK + 
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKE-GKLIKQYAMTSK-KA 491

Query: 486 KASITFEGTKYRSR-APIVAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELG 545
            AS+   GT+   + +P+VAAFSSRGP+F    ++KPD+ APGVNILAAW   ++P+ L 
Sbjct: 492 TASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLS 551

Query: 546 SDKRRVLFNIISGTSMSCPHVSGIAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISD 605
           SD RRV FNI+SGTSMSCPHVSG+AAL+KS H +WSPAAIKSALMTTAY+ DN  + ++D
Sbjct: 552 SDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD 611

Query: 606 VGRASGGPANAFALGSGHVDPEKAVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSR-GN 665
              A+  P++ +  G+GH+DP +A DPGL+YDI PQ+Y  +LC+ + +  Q+ + ++  N
Sbjct: 612 ASGAA--PSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSN 671

Query: 666 FTCPSKRTLLQ-PGDLNYPSFSVFMKKKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGI 725
            TC  K TL + PG+LNYP+ S    +      +TL+RTVTNVG   S Y V ++   G 
Sbjct: 672 RTC--KHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGA 731

Query: 726 TIIVKPEKLSFRRLGQKLSYQVSFVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTW 768
           ++ V+P+ L+F    QKLSY V+F    +   +    FG L+W S  + VRSP+ +TW
Sbjct: 732 SVTVQPKTLNFTSKHQKLSYTVTFRTRFR---MKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Tan0011054 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 672.5 bits (1734), Expect = 3.9e-193
Identity = 364/755 (48.21%), Postives = 487/755 (64.50%), Query Frame = 0

Query: 22  ADDQQTYIIHMDPTRMATTNP--EQWYTSMIDSVNELSSLDEDQEEASNAAEILYVYKTA 81
           + DQ TYI+HM  ++M ++      WY S + S+             S++AE+LY Y+ A
Sbjct: 26  SSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-------------SDSAELLYTYENA 85

Query: 82  LSGFSAKLSTRKLHLLSKIPGFVAATRDELLQLHTTHTPQFLGL-RRGHGLWNSSNMASD 141
           + GFS +L+  +   L   PG ++   +   +LHTT TP FLGL      L+  +   SD
Sbjct: 86  IHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSD 145

Query: 142 IIIGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRSNCNKKLIGARAYIKGYEA 201
           +++GV+D+G+WPE  S+ D+GF P+P  WKG CE G  F+ S CN+KLIGAR + +GYE+
Sbjct: 146 VVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYES 205

Query: 202 ILGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMGVAAGISFTSRIAAYKV 261
            +G ++ +   RS RD DGHGTHT+STAAG++V  ASL     G A G++  +R+A YKV
Sbjct: 206 TMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKV 265

Query: 262 CWPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEMAIATFGAVRNGVFVSCSA 321
           CW  GC S+DILAAID+A+ D V+VLS+SLGGG   +Y D +AI  F A+  G+ VSCSA
Sbjct: 266 CWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSA 325

Query: 322 GNSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFKGSSLYSGNNIDQ--LPLV 381
           GN+GP  S++ NVAPWI TV A   DR FPA   LGNG+ F G SL+ G  +    LP +
Sbjct: 326 GNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFI 385

Query: 382 Y-NNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQVKLAGGAGMILINIRL 441
           Y  N +     N+C  G+L+P  VKGKIV+C++G N+R++KG+ VK AGG GMIL N   
Sbjct: 386 YAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAA 445

Query: 442 EGEDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEGTKYRSR-APIVAAFS 501
            GE+  AD H+LPA T+G + AG  I +Y+ +  + P ASI+  GT    + +P+VAAFS
Sbjct: 446 NGEELVADAHLLPATTVGEK-AGDIIRHYVTTDPN-PTASISILGTVVGVKPSPVVAAFS 505

Query: 502 SRGPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFNIISGTSMSCPHVSG 561
           SRGP+   P ++KPD+ APGVNILAAW     PT L SD RRV FNIISGTSMSCPHVSG
Sbjct: 506 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 565

Query: 562 IAALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPANAFALGSGHVDPEK 621
           +AALLKSVH  WSPAAI+SALMTTAY T    + + D+  A+G P+  F  G+GHV P  
Sbjct: 566 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPSTPFDHGAGHVSPTT 625

Query: 622 AVDPGLIYDIAPQDYLNYLCSSNYTSKQVALVSRGNFTC-PSKRTLLQPGDLNYPSFSVF 681
           A +PGLIYD+  +DYL +LC+ NYTS Q+  VSR N+TC PSK       DLNYPSF+V 
Sbjct: 626 ATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS--YSVADLNYPSFAVN 685

Query: 682 MKKKAKNVIVTLKRTVTNVGSPRSDYTVKI-NNPTGITIIVKPEKLSFRRLGQKLSYQVS 741
           +            RTVT+VG     Y+VK+ +  TG+ I V+P  L+F+   +K SY V+
Sbjct: 686 VDGVG---AYKYTRTVTSVGG-AGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVT 745

Query: 742 FVALGKGKALSEFSFGSLIWLSGKYVVRSPIAVTW 768
           F  +   K     SFGS+ W  GK+VV SP+A++W
Sbjct: 746 F-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Tan0011054 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 652.9 bits (1683), Expect = 3.2e-187
Identity = 359/752 (47.74%), Postives = 467/752 (62.10%), Query Frame = 0

Query: 25  QQTYII---HMDPTRMATTNPEQWYTSMIDSVNELSSLDEDQEEASNAAEILYVYKTALS 84
           ++TYII   H D      T+   WYTS ++S + L                LY Y T+  
Sbjct: 27  KKTYIIRVNHSDKPESFLTH-HDWYTSQLNSESSL----------------LYTYTTSFH 86

Query: 85  GFSAKL-STRKLHLLSKIPGFVAATRDELLQLHTTHTPQFLGLRRGHGLWNSSNMASDII 144
           GFSA L ST    LLS     +    D L  LHTT TP+FLGL    G+ +  + ++ +I
Sbjct: 87  GFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVI 146

Query: 145 IGVIDSGIWPEHISFQDKGFPPVPKKWKGVCEVGQKFSRSNCNKKLIGARAYIKGYE-AI 204
           IGV+D+G+WPE  SF D   P +P KWKG CE G  F    CNKKLIGAR++ KG++ A 
Sbjct: 147 IGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMAS 206

Query: 205 LGRLNATGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMGVAAGISFTSRIAAYKVC 264
            G  ++     S RD DGHGTHT++TAAG+ V  AS      G A G++  +R+A YKVC
Sbjct: 207 GGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVC 266

Query: 265 WPEGCASADILAAIDRAVIDGVDVLSLSLGGGDGSFYNDEMAIATFGAVRNGVFVSCSAG 324
           W  GC  +DILAA+DRA++DGVDVLSLSLGGG   +Y D +AI  F A+  GVFVSCSAG
Sbjct: 267 WSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAG 326

Query: 325 NSGPFISTVVNVAPWIMTVAASYTDRTFPATLKLGNGQIFKGSSLYSGNNIDQLP--LVY 384
           NSGP  ++V NVAPW+MTV A   DR FPA   LGNG+   G SLYSG  +   P  LVY
Sbjct: 327 NSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY 386

Query: 385 NNTAGGEEANVCAAGSLVPSMVKGKIVVCEKGTNSRLEKGEQVKLAGGAGMILINIRLEG 444
           N       +N+C  GSL  S+V+GKIVVC++G N+R+EKG  V+ AGG GMI+ N    G
Sbjct: 387 NK-GNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASG 446

Query: 445 EDFFADPHVLPAITLGTRSAGKAIINYIASSKHQPKASITFEGTKYRSR-APIVAAFSSR 504
           E+  AD H+LPAI +G +  G  +  Y+ S   +P A + F+GT    + +P+VAAFSSR
Sbjct: 447 EELVADSHLLPAIAVG-KKTGDLLREYVKSDS-KPTALLVFKGTVLDVKPSPVVAAFSSR 506

Query: 505 GPSFFEPYVIKPDITAPGVNILAAWPSIVSPTELGSDKRRVLFNIISGTSMSCPHVSGIA 564
           GP+   P ++KPD+  PGVNILA W   + PT L  D RR  FNI+SGTSMSCPH+SG+A
Sbjct: 507 GPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLA 566

Query: 565 ALLKSVHKNWSPAAIKSALMTTAYITDNKRRLISDVGRASGGPANAFALGSGHVDPEKAV 624
            LLK+ H  WSP+AIKSALMTTAY+ DN    + D   A    +N +A GSGHVDP+KA+
Sbjct: 567 GLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDA--ADNSLSNPYAHGSGHVDPQKAL 626

Query: 625 DPGLIYDIAPQDYLNYLCSSNYTSKQ-VALVSRGNFTCPSKRTLLQPGDLNYPSFSVFMK 684
            PGL+YDI+ ++Y+ +LCS +YT    VA+V R +  C  K     PG LNYPSFSV   
Sbjct: 627 SPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK--FSDPGQLNYPSFSVLFG 686

Query: 685 KKAKNVIVTLKRTVTNVGSPRSDYTVKINNPTGITIIVKPEKLSFRRLGQKLSYQVSFVA 744
            K    +V   R VTNVG+  S Y V +N    + I VKP KLSF+ +G+K  Y V+FV+
Sbjct: 687 GKR---VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVS 746

Query: 745 LGKGKALSEFSFGSLIWLSGKYVVRSPIAVTW 768
                  ++  FGS+ W + ++ VRSP+A +W
Sbjct: 747 KKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751