Tan0010682 (gene) Snake gourd v1

BLAST of Tan0010682 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 4.7e-142
Identity = 284/508 (55.91%), Postives = 362/508 (71.26%), Query Frame = 0

Query: 7   SLYISLLLILVSLFPFGSPSE---GTTFSIEEATLKDLQLAFNQNKLTSRQLVEFYIEQI 66
           SL I L+L    +   GS S+    +TFSI+EAT++D+++AFN+ +LTS+QLVE Y+E I
Sbjct: 11  SLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAI 70

Query: 67  RKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATKDKLRTT 126
            K N  L  VIE NPDA   A+ ADRER    T + LP+LHG+PVL+KD+I+TKDKL TT
Sbjct: 71  SKLNPILHAVIETNPDALIQAEIADRERDLKNT-TKLPILHGVPVLLKDSISTKDKLNTT 130

Query: 127 AGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNGRGYEAY 186
           AGSFALL S+V RDAGVV +LR++GA+ILGKASLS+W+ FRS   P GWSAR  +G   Y
Sbjct: 131 AGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNPY 190

Query: 187 TF-GDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSRAGVVP 246
               +P GSSSGSAISV+ N+ AV++GTETDGS+L P+S NSVV IKP+VGLTSRAGVVP
Sbjct: 191 VLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVVP 250

Query: 247 ISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDGLRGKR 306
           IS RQD++GP+ RTV+DA ++L+AIVGYD  D+AT   S++IP GGY QFL   GL+GKR
Sbjct: 251 ISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKR 310

Query: 307 LGIVQDLSLGVDPSVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGEWTALLNE 366
           LGIV   S  +D  +          LR +GAI++NNLTI +   I    +SGE  ALL E
Sbjct: 311 LGIVMKHSSLLDHHI--------KTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAE 370

Query: 367 FRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGTGMGE--I 426
           F+ SLNAYL+ELV SPVRSL+DVI +N    + E+V  +GQ     A AT+G G  E   
Sbjct: 371 FKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTA 430

Query: 427 LAKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTRKPFGFYF 486
           L K+  LS+ G EK + +N+LDAI++ G  +   LAIGG+PGI+VPAGY     P+G  F
Sbjct: 431 LQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISF 490

Query: 487 GGLKGYEPRLIEIAYAFEQATKRRKPPR 509
           GGL+  EP+LIEIA+AFEQAT  RKPP+
Sbjct: 491 GGLRFSEPKLIEIAFAFEQATLIRKPPK 509

BLAST of Tan0010682 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 7.6e-68
Identity = 181/519 (34.87%), Postives = 265/519 (51.06%), Query Frame = 0

Query: 32  SIEEATLKDLQLAFNQNKLTSRQLVEFYIEQIRKYNGRLRGVIEVNPDARDLADNADRER 91
           ++E+AT+  LQ       LTS  +V  Y+++  + N  + G++++NPD   +A   D ER
Sbjct: 65  TLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDER 124

Query: 92  KANKTPSALPLLHGIPVLVKDNIATKDKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAII 151
                      LHGIP +VKDN ATKDK+ TTAGS+ALL SIVPRDA VV +LR+AGA++
Sbjct: 125 ANGIIRGP---LHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVL 184

Query: 152 LGKASLSQWSGFRSRLAPTGWSARNGRGYEAYTFG-DPCGSSSGSAISVSTNMAAVTVGT 211
            G A+LS+W+  RS     G+SAR G+    +    +P GSSSGSAISV++NM A  +GT
Sbjct: 185 FGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGT 244

Query: 212 ETDGSLLCPSSYNSVVTIKPTVGLTSRAGVVPISRRQDTVGPMGRTVADAAYVLEAIVGY 271
           ETDGS++ P+  N VV +KPTVGLTSR GV+P S  QDT GP+ RTV DA YV +++ G 
Sbjct: 245 ETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGI 304

Query: 272 DVFDQAT-NCCSQYIPSGGYGQFL-KPDGLRGKRLGIV-QDLSLGVDPSVAQAFQDVFPI 331
           D  D  T N   +    G Y +FL     L G R G+  + L             +V   
Sbjct: 305 DENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLEVVKQ 364

Query: 332 LRGKGAILVNNLTIKSFVTIAN--------PFESGEWTALLNEFRESLNAYLQELVSSPV 391
           +   GAI+ NN    +   I+N             E+T +  +F  ++ +YL E+ ++ +
Sbjct: 365 IEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKNTEI 424

Query: 392 RSLSDVIQFNNNHKDLEE-----VNTYGQGY------LEAANATNGTGMGEILAKLTTLS 451
            SL D++++NN +   E      V  +  G       LE     N T    +     T  
Sbjct: 425 HSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRRTSQ 484

Query: 452 KEGFE--------KTMIKNELDAIISPG--ELIFPFLAIGGFPGISVPAGYSPTRKPFGF 511
            EG +        KT     L+ ++ P    + +   A  G+P I++P G     +PFG 
Sbjct: 485 DEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGRPFGL 544

Query: 512 YFGGLKGYEPRLIEIAYAFEQATKRRKPPRLSKLARSSV 518
                   EP+LI+   A E   + +  P+  +    +V
Sbjct: 545 GIMHSAWQEPQLIKYGSAIEDLLQYKAKPKFYEYVAKNV 580

BLAST of Tan0010682 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.8e-65
Identity = 178/510 (34.90%), Postives = 263/510 (51.57%), Query Frame = 0

Query: 41  LQLAFNQNKLTSRQLVEFYIEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSAL 100
           LQ  + Q  +    +V+ Y+ +I + N  +R V E+NPDA  +A   D ERK  K     
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLRGP- 153

Query: 101 PLLHGIPVLVKDNIATKDKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQW 160
             LHG+P+++K+NI T DK+ +TAGS+A+  +    DA V TKLR+AG +I+GK+  SQW
Sbjct: 154 --LHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQW 213

Query: 161 SGFRSRLAPTGWSARNGRGYEAYTFG-DPCGSSSGSAISVSTNMAAVTVGTETDGSLLCP 220
           + FRS  +  GWSA  G+   AY    DP GSSSGS ++    +A  T+GTET GS++ P
Sbjct: 214 ANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSP 273

Query: 221 SSYNSVVTIKPTVGLTSRAGVVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNC 280
           +  +++V +KPTVGLTSR  VVPIS RQDTVGPM R+V DAAY+L+ I G D  D  T+ 
Sbjct: 274 ADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYTSA 333

Query: 281 CSQYIPSGGYGQFLKP---DGLRGKRLGIVQDL--SLGVDPSVAQAFQDVFPILRGKGAI 340
               IP      ++K    + L+GKR+G+ +++    G   +V   F     +++  GAI
Sbjct: 334 ----IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAI 393

Query: 341 LVNNLTIKSFVTIA-NPFESGEWTALLNEFRESLNAYLQELVSSP--VRSLSDVIQFNNN 400
           +V N    SF   A +P        L  +   +L A+ ++L  +P  +  L  + +F  +
Sbjct: 394 IVENTDFTSFAEFAQSPIPD---DILYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQH 453

Query: 401 HKDLEEVNTYGQG----YLEAANATNGTGMGEILAKLTTLSKE-GFEKTMIKNELDAIIS 460
           H+ LEE  +         L+            +  K      E G    + +++LDA + 
Sbjct: 454 HR-LEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVL 513

Query: 461 PGELIFPFLAIGGFPGISVPAGYSPTRK---------------PFGFYFGGLKGYEPRLI 520
           P +L     A+ G P I+VP G  P                  P G  F G    E +LI
Sbjct: 514 PTDLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLI 573

Query: 521 EIAYAFEQATKRRKPPRLSKLARSSVSTQG 522
            +AYAFEQ T  R  P+L +  +     +G
Sbjct: 574 GLAYAFEQKTHAR--PKLKRFIQPKKEVKG 590

BLAST of Tan0010682 vs. ExPASy Swiss-Prot
Match: B0JSX3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=gatA PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 3.1e-37
Identity = 140/501 (27.94%), Postives = 226/501 (45.11%), Query Frame = 0

Query: 37  TLKDLQLAFNQNKLTSRQLVEFYIEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKT 96
           +++ L       + T+ ++   ++ +I+    +++  + + PD   LA     + K  + 
Sbjct: 3   SIRQLHQQLVNKEKTAVEIATEFLARIQALEPQVKSFLHLTPDLA-LAQAQKVDEKIARG 62

Query: 97  PSALPLLHGIPVLVKDNIATKDKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKAS 156
            S L LL GIP+ +KDN+ TK  + TT  S  L N + P ++ V  KLR  GA+I+GK +
Sbjct: 63  ES-LHLLAGIPIALKDNLCTKG-IPTTCASRILENFVPPYESTVTQKLRDLGAVIVGKTN 122

Query: 157 LSQWSGFRSRLAPTGWSARNGRGYEAYTFGD----PCGSSSGSAISVSTNMAAVTVGTET 216
           L +++         G S  N   +      D    P GSS GSA +V+     V +G++T
Sbjct: 123 LDEFA--------MGSSTENSGYHVTANPWDLSRVPGGSSGGSAAAVAAQECVVALGSDT 182

Query: 217 DGSLLCPSSYNSVVTIKPTVGLTSRAGVVPISRRQDTVGPMGRTVADAAYVLEAIVGYDV 276
            GS+  P+S+  VV +KPT GL SR G+V  +   D +GP GRTV DAA +L+AI GYD 
Sbjct: 183 GGSIRQPASFCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQAIAGYDP 242

Query: 277 FDQATNCCSQYIPSGGYGQFLKPDGLRGKRLGIVQD-LSLGVDPSVAQAFQDVFPILRGK 336
            D      S  +P   Y QFLK   L+G ++G++++    G+D  VA+A       L+  
Sbjct: 243 QDST----SLNLPIPDYSQFLK-TSLKGLKIGVIKETFGEGLDQVVAEAVNQALAQLKAL 302

Query: 337 GAILVN------NLTIKSFVTIANPFESGEWTALLNEFRESLNAYLQELVSSPVRSLSDV 396
           GA +           + ++  IA P E+    A  +  +  +      L+    ++ +  
Sbjct: 303 GATIKEISCPRFRYGLPTYYIIA-PSEASANLARYDGVKYGIREDADSLIDMYTKTRAKG 362

Query: 397 IQFNNNHKDLEEVNTYGQGYLEAANATNGTGMGEILAKLTTLSKEGFEKTMIKNELDAII 456
                  + +    T   GY +A              K+ TL KE F++      +D ++
Sbjct: 363 FGAEVKRRIMLGTYTLSAGYYDAYYLK--------AQKVRTLIKEDFDRAF--QSVDVLV 422

Query: 457 SPGELIFPFLA---------------------IGGFPGISVPAGYSPTRKPFGFYFGGLK 506
           SP      F A                     + G PG+S+P G+     P G    G  
Sbjct: 423 SPTSPTTAFKAGEKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDGQGLPIGLQLVGNV 476

BLAST of Tan0010682 vs. ExPASy Swiss-Prot
Match: B1WYP3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=gatA PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 6.9e-37
Identity = 141/501 (28.14%), Postives = 232/501 (46.31%), Query Frame = 0

Query: 37  TLKDLQLAFNQNKLTSRQLVEFYIEQIRKYNGRLRGVIEVNPD-ARDLADNADRERKANK 96
           ++++L       + ++ ++    +E+I   + +++  ++V  D A + A   D +   N+
Sbjct: 3   SIRELHQQLVNKERSAVEITTEALERINAVDSKVKSFLQVTADHALETAKKVDEKIAKNE 62

Query: 97  TPSALPLLHGIPVLVKDNIATKDKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKA 156
               + LL GIP+ +KDN+ T   ++TT GS  L N + P ++ V  KL+ AGA+++GK 
Sbjct: 63  ---EIGLLAGIPIGIKDNMCTTG-IKTTCGSKILANFVPPYESTVTQKLKDAGAVMVGKT 122

Query: 157 SLSQWSGFRSRLAPTGWSARNGRGYEAYT-----FGDPCGSSSGSAISVSTNMAAVTVGT 216
           +L +++         G S  N  GY+           P GSS GSA +V+ +   V +G+
Sbjct: 123 NLDEFA--------MGSSTENS-GYQVTANPWDLSRVPGGSSGGSAAAVAASECVVALGS 182

Query: 217 ETDGSLLCPSSYNSVVTIKPTVGLTSRAGVVPISRRQDTVGPMGRTVADAAYVLEAIVGY 276
           +T GS+  P+S   VV +KPT GL SR G+V  +   D +GP  R V DAA +L AI GY
Sbjct: 183 DTGGSIRQPASLCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFARNVQDAAILLGAIAGY 242

Query: 277 DVFDQATNCCSQYIPSGGYGQFLKPDGLRGKRLGIVQD-LSLGVDPSVAQAFQDVFPILR 336
           D  D      S   P   Y QFLKP  L G  +G++++    G+DP V++  +     L+
Sbjct: 243 DPKDST----SINTPVPDYTQFLKP-SLEGLTIGVIEETFGEGLDPVVSKTVKAAIDHLK 302

Query: 337 GKGAILVN------NLTIKSFVTIANPFESGEWTALLNEFRESLNAYLQELVSSPVRSLS 396
             GA +VN         + ++  IA P E+    A  +  +  +      L+    ++ +
Sbjct: 303 TLGAKVVNVSCPRFRYGLPAYYIIA-PSEASSNLARYDAVKYGIREDADTLIDMYTKTRA 362

Query: 397 DVIQFNNNHKDLEEVNTY--GQGYLEAANATNGTGMGEILAKLTTLSKEGFEKTMIKNEL 456
             + F N  K    + TY    GY +A              K+ TL K+ F+    K  +
Sbjct: 363 --VGFGNEVKRRIMIGTYTLSAGYYDAYYLK--------AQKVRTLIKQDFDAGFEK--V 422

Query: 457 DAIISPGELIFPFLA---------------------IGGFPGISVPAGYSPTRKPFGFYF 502
           D ++ P      F A                     + G PG+S+P G+     P G   
Sbjct: 423 DVLVCPTSPTTAFKAGEKTSDPLSMYLSDLTTIPVNLAGLPGMSIPCGFDDNNLPVGLQL 472

BLAST of Tan0010682 vs. NCBI nr
Match: XP_022146229.1 (putative amidase C869.01 [Momordica charantia])

HSP 1 Score: 666.0 bits (1717), Expect = 2.7e-187
Identity = 356/510 (69.80%), Postives = 405/510 (79.41%), Query Frame = 0

Query: 1   MEPKSFSLYISLLLILVSLFPFGSPS-EGTTFSIEEATLKDLQLAFNQNKLTSRQLVEFY 60
           ME    SL  SL LILV++ PF S S     F IEEA LKDL LAF QNKLTSRQLVEFY
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60

Query: 61  IEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATKDK 120
           I+QIR+YN RLRGVIEVNPDA  LAD ADRERKA K P +LPLLHGIPVLVKDN+ATKDK
Sbjct: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKA-KAPGSLPLLHGIPVLVKDNMATKDK 120

Query: 121 LRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNGRG 180
           L TTAGS ALL S+VPRDAG V +LRKAGAIILGKAS+S+W+GFRS  APTGW+AR G+G
Sbjct: 121 LNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQG 180

Query: 181 YEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSRAG 240
            E YT G PCGSSSGSAISVS NMAAV +GTETDGS+LCPSS+NSVV IKPT+GLTS AG
Sbjct: 181 REPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTAG 240

Query: 241 VVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDGLR 300
           VVPIS RQDTVGP+ RTV+DAAYVL+ IVG D  D +T   S+YIP GGYGQFLK DGLR
Sbjct: 241 VVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLR 300

Query: 301 GKRLGIVQDLSLGVDPSVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGEWTAL 360
           GKRLGIV+D    VDPS+  AF+++F IL  +GAILV+NL I +   I N  ESGE  AL
Sbjct: 301 GKRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAAL 360

Query: 361 LNEFRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGTGMGE 420
           LNEF+ SLNAYL+ELVSSP+RSL++ I FN  H  LE++  YGQ     A ATNG G+  
Sbjct: 361 LNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGING 420

Query: 421 ILAKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTRKPFGFY 480
           +L+KL  LSK+G EKTMIKN+LDAI++P  +I P LAIGGFPGI+VPAGYSP+  PFG  
Sbjct: 421 LLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGID 480

Query: 481 FGGLKGYEPRLIEIAYAFEQATKRRKPPRL 510
           FGGLKGYEP+LIEIAY FEQATKRR+ P L
Sbjct: 481 FGGLKGYEPKLIEIAYGFEQATKRRRKPPL 509

BLAST of Tan0010682 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 619.4 bits (1596), Expect = 2.9e-173
Identity = 341/518 (65.83%), Postives = 394/518 (76.06%), Query Frame = 0

Query: 1   MEPKSFSLYISL---LLILVSLFPFGSPSEGTTFSIEEATLKDLQLAFNQNKLTSRQLVE 60
           M  +SF +YIS+   LL ++SL+  GS S  T FSIEEATLKD QLAF QNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYIEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATK 120
           FY+EQ+R+ N  L+G+IEVNPDA + A  AD +RK +   S  P LHGIPVLVKDNIATK
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIATK 120

Query: 121 DKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNG 180
           DKL TTAGSFALL SIVPRDAGVVTKLRKAGAII GKASLS+WSGFRS   P GWSAR G
Sbjct: 121 DKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGG 180

Query: 181 RGYEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSR 240
           +G   YT G+PCGSSSGSAISV+ NM  V++GTETDGS+LCPS+ NSVV IKPTVGLTSR
Sbjct: 181 QGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSR 240

Query: 241 AGVVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDG 300
           AGVVPIS RQDTVGP+ RTVADAAYVL+AI G D +D +T   S+YIP GGYGQFL+ +G
Sbjct: 241 AGVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEG 300

Query: 301 LRGKRLGIVQDL-SLGVDP-SVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGE 360
           L+GKR+GIV+ L   G D      AF+ VF  L+  GAILV+NLTI  F  I     SGE
Sbjct: 301 LKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS-SSGE 360

Query: 361 WTALLNEFRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGT 420
           WTALL EF+ SLNAYL++LV+SP+RSLSD I+FN  +  LE++  YGQ     A AT G 
Sbjct: 361 WTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGI 420

Query: 421 GMGE--ILAKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTR 480
           G  E   LA+L  LSKEGFE+ MIKN+LDAI +PG LI PFLAIGGFPG+SVPAGY+P  
Sbjct: 421 GGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQG 480

Query: 481 KPFGFYFGGLKGYEPRLIEIAYAFEQATKRRKPPRLSK 512
            PFG  FGGLKG++PRLIEIAY FE  T  RK P L +
Sbjct: 481 LPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of Tan0010682 vs. NCBI nr
Match: XP_038900913.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 617.8 bits (1592), Expect = 8.4e-173
Identity = 338/516 (65.50%), Postives = 399/516 (77.33%), Query Frame = 0

Query: 1   MEPKSFSLYISLLLILVS-LFPFGSPSEGTTFSIEEATLKDLQLAFNQNKLTSRQLVEFY 60
           M  +SF LYIS++L LV+ L  +G+ S GT F IEEAT+KDLQLAF QNKLTSRQLV+FY
Sbjct: 1   MAAQSFWLYISMVLSLVAILSSYGNCSFGTHFPIEEATVKDLQLAFYQNKLTSRQLVKFY 60

Query: 61  IEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATKDK 120
           ++Q+R++N  L+G+IEVNPDA D A  AD ERK +   S  P LHGIPVL+KDNIATKDK
Sbjct: 61  LKQVRRFNPILKGIIEVNPDALDQATRADHERKRSSLSSLSP-LHGIPVLLKDNIATKDK 120

Query: 121 LRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNGRG 180
           L TTAGSFALL SIVPRDAGVVTKLRKAGAII GKASLS+WS FRS L P GW AR G+G
Sbjct: 121 LNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSQFRSNL-PNGWCARGGQG 180

Query: 181 YEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSRAG 240
              YT G+PCGSSSGSAISV+ NM AV++GT+TDGS+LCPS+YNSVV I+PTVGLTSRAG
Sbjct: 181 KNPYTMGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTYNSVVGIRPTVGLTSRAG 240

Query: 241 VVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDGLR 300
           VVPIS RQD VGP+ RTV+DAAYVL+AIVG D +D +T   S+YIP GGYGQFL+PDGL+
Sbjct: 241 VVPISSRQDAVGPICRTVSDAAYVLDAIVGADKYDNSTIEASKYIPKGGYGQFLRPDGLK 300

Query: 301 GKRLGIVQDLSLGVDPSV--AQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGEWT 360
           G R+GIV+      D  V  ++A++ VF  L+  GAILV+NLTI S   I     SGEWT
Sbjct: 301 GTRIGIVRKFFDFGDDGVFYSEAYEKVFKSLKQGGAILVDNLTIDSLDIIIGGL-SGEWT 360

Query: 361 ALLNEFRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGTGM 420
           AL  EF+ S+NAYL+ELV+SP+RSLSD I+FN  +  LE++  YGQ     A ATNG G 
Sbjct: 361 ALPAEFKISINAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQVLFLEAEATNGIGD 420

Query: 421 GE--ILAKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTRKP 480
            E   LA + +LSK GFE+ MIKN+LDAI +PG LI P LAIGGFPGISVPAGYSP   P
Sbjct: 421 VEKAALASVASLSKNGFERVMIKNKLDAIAAPGGLISPVLAIGGFPGISVPAGYSPDGVP 480

Query: 481 FGFYFGGLKGYEPRLIEIAYAFEQATKRRKPPRLSK 512
           +G  FGGLKG+EPRLIEIAY FEQ TK RKPP + +
Sbjct: 481 YGIGFGGLKGFEPRLIEIAYGFEQLTKSRKPPLIKR 513

BLAST of Tan0010682 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 616.7 bits (1589), Expect = 1.9e-172
Identity = 339/516 (65.70%), Postives = 392/516 (75.97%), Query Frame = 0

Query: 1   MEPKSFSLYISLLL-ILVSLFPFGSPSEGTTFSIEEATLKDLQLAFNQNKLTSRQLVEFY 60
           M  +SF +YIS+LL +L  L  +GS S  T FSIEEATLKDLQLAF QNKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  IEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATKDK 120
           +EQ+R++N  L G+IEVNPDA + A  AD ERK   +P +L  LHGIPVLVKDNIATKD+
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERK-RSSPRSLSPLHGIPVLVKDNIATKDQ 120

Query: 121 LRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNGRG 180
           L TTAGSFALL SIVPRDAGVVTKLRKAGAII GKASLS+WS FRS   P GWSAR G+G
Sbjct: 121 LNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQG 180

Query: 181 YEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSRAG 240
              YT G+PCGSSSGSAISV+ NM  V++GTETDGS+LCPS+ NSVV IKPTVGLTSRAG
Sbjct: 181 KNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAG 240

Query: 241 VVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDGLR 300
           VVPIS RQDTVGP+ RTVADAAYVL+AI G D +D +T   S+Y+P GGYGQFLK DGL+
Sbjct: 241 VVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLK 300

Query: 301 GKRLGIVQDL-SLGVDP-SVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGEWT 360
           GKR+GIV+ L   G D      AF+ VF  L+  GAILV+NLTI SF  I     SGEWT
Sbjct: 301 GKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGS-SSGEWT 360

Query: 361 ALLNEFRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGTGM 420
           A+L EF+ S+N YL++LV+SP+RSLSD I+FN  +  LE++  YGQ     A AT G G 
Sbjct: 361 AVLAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGG 420

Query: 421 GE--ILAKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTRKP 480
            E   LA+L  LSKEGFE+ MIKN+LDAI +PG LI  FLAIGGFPG+SVPAGY+P   P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGFYFGGLKGYEPRLIEIAYAFEQATKRRKPPRLSK 512
           FG  FGGLKG+EPRLIEIAY FE  T  RK P L +
Sbjct: 481 FGIGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of Tan0010682 vs. NCBI nr
Match: ADN34007.1 (amidase [Cucumis melo subsp. melo])

HSP 1 Score: 612.8 bits (1579), Expect = 2.7e-171
Identity = 335/503 (66.60%), Postives = 385/503 (76.54%), Query Frame = 0

Query: 13  LLILVSLFPFGSPSEGTTFSIEEATLKDLQLAFNQNKLTSRQLVEFYIEQIRKYNGRLRG 72
           LL ++SL+  GS S  T FSIEEATLKD QLAF QNKLTSRQLVEFY+EQ+R+ N  L+G
Sbjct: 5   LLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNPILKG 64

Query: 73  VIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATKDKLRTTAGSFALLNS 132
           +IEVNPDA + A  AD +RK +   S  P LHGIPVLVKDNIATKDKL TTAGSFALL S
Sbjct: 65  IIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIATKDKLNTTAGSFALLGS 124

Query: 133 IVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNGRGYEAYTFGDPCGSS 192
           IVPRDAGVVTKLRKAGAII GKASLS+WSGFRS   P GWSAR G+G   YT G+PCGSS
Sbjct: 125 IVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPCGSS 184

Query: 193 SGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSRAGVVPISRRQDTVGP 252
           SGSAISV+ NM  V++GTETDGS+LCPS+ NSVV IKPTVGLTSRAGVVPIS RQDTVGP
Sbjct: 185 SGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGP 244

Query: 253 MGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDGLRGKRLGIVQDL-SL 312
           + RTVADAAYVL+AI G D +D +T   S+YIP GGYGQFL+ +GL+GKR+GIV+ L   
Sbjct: 245 ICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKLYDF 304

Query: 313 GVDP-SVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGEWTALLNEFRESLNAY 372
           G D      AF+ VF  L+  GAILV+NLTI  F  I     SGEWTALL EF+ SLNAY
Sbjct: 305 GHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS-SSGEWTALLAEFKISLNAY 364

Query: 373 LQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGTGMGE--ILAKLTTLS 432
           L++LV+SP+RSLSD I+FN  +  LE++  YGQ     A AT G G  E   LA+L  LS
Sbjct: 365 LKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARLAKLS 424

Query: 433 KEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTRKPFGFYFGGLKGYEP 492
           KEGFE+ MIKN+LDAI +PG LI PFLAIGGFPG+SVPAGY+P   PFG  FGGLKG++P
Sbjct: 425 KEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLKGFDP 484

Query: 493 RLIEIAYAFEQATKRRKPPRLSK 512
           RLIEIAY FE  T  RK P L +
Sbjct: 485 RLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of Tan0010682 vs. ExPASy TrEMBL
Match: A0A6J1CXI8 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015494 PE=4 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 1.3e-187
Identity = 356/510 (69.80%), Postives = 405/510 (79.41%), Query Frame = 0

Query: 1   MEPKSFSLYISLLLILVSLFPFGSPS-EGTTFSIEEATLKDLQLAFNQNKLTSRQLVEFY 60
           ME    SL  SL LILV++ PF S S     F IEEA LKDL LAF QNKLTSRQLVEFY
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60

Query: 61  IEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATKDK 120
           I+QIR+YN RLRGVIEVNPDA  LAD ADRERKA K P +LPLLHGIPVLVKDN+ATKDK
Sbjct: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKA-KAPGSLPLLHGIPVLVKDNMATKDK 120

Query: 121 LRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNGRG 180
           L TTAGS ALL S+VPRDAG V +LRKAGAIILGKAS+S+W+GFRS  APTGW+AR G+G
Sbjct: 121 LNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQG 180

Query: 181 YEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSRAG 240
            E YT G PCGSSSGSAISVS NMAAV +GTETDGS+LCPSS+NSVV IKPT+GLTS AG
Sbjct: 181 REPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTAG 240

Query: 241 VVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDGLR 300
           VVPIS RQDTVGP+ RTV+DAAYVL+ IVG D  D +T   S+YIP GGYGQFLK DGLR
Sbjct: 241 VVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLR 300

Query: 301 GKRLGIVQDLSLGVDPSVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGEWTAL 360
           GKRLGIV+D    VDPS+  AF+++F IL  +GAILV+NL I +   I N  ESGE  AL
Sbjct: 301 GKRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAAL 360

Query: 361 LNEFRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGTGMGE 420
           LNEF+ SLNAYL+ELVSSP+RSL++ I FN  H  LE++  YGQ     A ATNG G+  
Sbjct: 361 LNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGING 420

Query: 421 ILAKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTRKPFGFY 480
           +L+KL  LSK+G EKTMIKN+LDAI++P  +I P LAIGGFPGI+VPAGYSP+  PFG  
Sbjct: 421 LLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGID 480

Query: 481 FGGLKGYEPRLIEIAYAFEQATKRRKPPRL 510
           FGGLKGYEP+LIEIAY FEQATKRR+ P L
Sbjct: 481 FGGLKGYEPKLIEIAYGFEQATKRRRKPPL 509

BLAST of Tan0010682 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.4e-173
Identity = 341/518 (65.83%), Postives = 394/518 (76.06%), Query Frame = 0

Query: 1   MEPKSFSLYISL---LLILVSLFPFGSPSEGTTFSIEEATLKDLQLAFNQNKLTSRQLVE 60
           M  +SF +YIS+   LL ++SL+  GS S  T FSIEEATLKD QLAF QNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYIEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATK 120
           FY+EQ+R+ N  L+G+IEVNPDA + A  AD +RK +   S  P LHGIPVLVKDNIATK
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIATK 120

Query: 121 DKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNG 180
           DKL TTAGSFALL SIVPRDAGVVTKLRKAGAII GKASLS+WSGFRS   P GWSAR G
Sbjct: 121 DKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGG 180

Query: 181 RGYEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSR 240
           +G   YT G+PCGSSSGSAISV+ NM  V++GTETDGS+LCPS+ NSVV IKPTVGLTSR
Sbjct: 181 QGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSR 240

Query: 241 AGVVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDG 300
           AGVVPIS RQDTVGP+ RTVADAAYVL+AI G D +D +T   S+YIP GGYGQFL+ +G
Sbjct: 241 AGVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEG 300

Query: 301 LRGKRLGIVQDL-SLGVDP-SVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGE 360
           L+GKR+GIV+ L   G D      AF+ VF  L+  GAILV+NLTI  F  I     SGE
Sbjct: 301 LKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS-SSGE 360

Query: 361 WTALLNEFRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGT 420
           WTALL EF+ SLNAYL++LV+SP+RSLSD I+FN  +  LE++  YGQ     A AT G 
Sbjct: 361 WTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGI 420

Query: 421 GMGE--ILAKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTR 480
           G  E   LA+L  LSKEGFE+ MIKN+LDAI +PG LI PFLAIGGFPG+SVPAGY+P  
Sbjct: 421 GGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQG 480

Query: 481 KPFGFYFGGLKGYEPRLIEIAYAFEQATKRRKPPRLSK 512
            PFG  FGGLKG++PRLIEIAY FE  T  RK P L +
Sbjct: 481 LPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of Tan0010682 vs. ExPASy TrEMBL
Match: A0A1S3C7A8 (putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.4e-173
Identity = 341/518 (65.83%), Postives = 394/518 (76.06%), Query Frame = 0

Query: 1   MEPKSFSLYISL---LLILVSLFPFGSPSEGTTFSIEEATLKDLQLAFNQNKLTSRQLVE 60
           M  +SF +YIS+   LL ++SL+  GS S  T FSIEEATLKD QLAF QNKLTSRQLVE
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYIEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATK 120
           FY+EQ+R+ N  L+G+IEVNPDA + A  AD +RK +   S  P LHGIPVLVKDNIATK
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIATK 120

Query: 121 DKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNG 180
           DKL TTAGSFALL SIVPRDAGVVTKLRKAGAII GKASLS+WSGFRS   P GWSAR G
Sbjct: 121 DKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGG 180

Query: 181 RGYEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSR 240
           +G   YT G+PCGSSSGSAISV+ NM  V++GTETDGS+LCPS+ NSVV IKPTVGLTSR
Sbjct: 181 QGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSR 240

Query: 241 AGVVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDG 300
           AGVVPIS RQDTVGP+ RTVADAAYVL+AI G D +D +T   S+YIP GGYGQFL+ +G
Sbjct: 241 AGVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEG 300

Query: 301 LRGKRLGIVQDL-SLGVDP-SVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGE 360
           L+GKR+GIV+ L   G D      AF+ VF  L+  GAILV+NLTI  F  I     SGE
Sbjct: 301 LKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS-SSGE 360

Query: 361 WTALLNEFRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGT 420
           WTALL EF+ SLNAYL++LV+SP+RSLSD I+FN  +  LE++  YGQ     A AT G 
Sbjct: 361 WTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGI 420

Query: 421 GMGE--ILAKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTR 480
           G  E   LA+L  LSKEGFE+ MIKN+LDAI +PG LI PFLAIGGFPG+SVPAGY+P  
Sbjct: 421 GGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQG 480

Query: 481 KPFGFYFGGLKGYEPRLIEIAYAFEQATKRRKPPRLSK 512
            PFG  FGGLKG++PRLIEIAY FE  T  RK P L +
Sbjct: 481 LPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of Tan0010682 vs. ExPASy TrEMBL
Match: A0A0A0LLK0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=4 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 2.6e-172
Identity = 338/516 (65.50%), Postives = 391/516 (75.78%), Query Frame = 0

Query: 1   MEPKSFSLYISLLL-ILVSLFPFGSPSEGTTFSIEEATLKDLQLAFNQNKLTSRQLVEFY 60
           M  +SF +YIS+LL +L  L  +GS S  T FSIEEATLKDLQLAF QNKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  IEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATKDK 120
           +EQ+R++N  L G+IEVNPDA + A  AD ERK   +P +L  LHGIPV VKDNIATKD+
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERK-RSSPRSLSPLHGIPVFVKDNIATKDQ 120

Query: 121 LRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNGRG 180
           L TTAGSFALL SIVPRDAGVVTKLRKAGAII GKASLS+WS FRS   P GWSAR G+G
Sbjct: 121 LNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQG 180

Query: 181 YEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSRAG 240
              YT G+PCGSSSGSAISV+ NM  V++GTETDGS+LCPS+ NSVV IKPTVGLTSRAG
Sbjct: 181 KNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAG 240

Query: 241 VVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDGLR 300
           VVPIS RQDTVGP+ RTVADAAYVL+AI G D +D +T   S+Y+P GGYGQFLK DGL+
Sbjct: 241 VVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLK 300

Query: 301 GKRLGIVQDL-SLGVDP-SVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGEWT 360
           GKR+GIV+ L   G D      AF+ VF  L+  GAILV+NLTI SF  I     SGEWT
Sbjct: 301 GKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGS-SSGEWT 360

Query: 361 ALLNEFRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGTGM 420
           A+L EF+ S+N YL++LV+SP+RSLSD I+FN  +  LE++  YGQ     A AT G G 
Sbjct: 361 AVLAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGG 420

Query: 421 GE--ILAKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTRKP 480
            E   LA+L  LSKEGFE+ MIKN+LDAI +PG LI  FLAIGGFPG+SVPAGY+P   P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGFYFGGLKGYEPRLIEIAYAFEQATKRRKPPRLSK 512
           FG  FGGLKG+EPRLIEIAY FE  T  RK P L +
Sbjct: 481 FGIGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of Tan0010682 vs. ExPASy TrEMBL
Match: E5GC08 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 1.3e-171
Identity = 335/503 (66.60%), Postives = 385/503 (76.54%), Query Frame = 0

Query: 13  LLILVSLFPFGSPSEGTTFSIEEATLKDLQLAFNQNKLTSRQLVEFYIEQIRKYNGRLRG 72
           LL ++SL+  GS S  T FSIEEATLKD QLAF QNKLTSRQLVEFY+EQ+R+ N  L+G
Sbjct: 5   LLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNPILKG 64

Query: 73  VIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATKDKLRTTAGSFALLNS 132
           +IEVNPDA + A  AD +RK +   S  P LHGIPVLVKDNIATKDKL TTAGSFALL S
Sbjct: 65  IIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIATKDKLNTTAGSFALLGS 124

Query: 133 IVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNGRGYEAYTFGDPCGSS 192
           IVPRDAGVVTKLRKAGAII GKASLS+WSGFRS   P GWSAR G+G   YT G+PCGSS
Sbjct: 125 IVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPCGSS 184

Query: 193 SGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSRAGVVPISRRQDTVGP 252
           SGSAISV+ NM  V++GTETDGS+LCPS+ NSVV IKPTVGLTSRAGVVPIS RQDTVGP
Sbjct: 185 SGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGP 244

Query: 253 MGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDGLRGKRLGIVQDL-SL 312
           + RTVADAAYVL+AI G D +D +T   S+YIP GGYGQFL+ +GL+GKR+GIV+ L   
Sbjct: 245 ICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKLYDF 304

Query: 313 GVDP-SVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGEWTALLNEFRESLNAY 372
           G D      AF+ VF  L+  GAILV+NLTI  F  I     SGEWTALL EF+ SLNAY
Sbjct: 305 GHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGS-SSGEWTALLAEFKISLNAY 364

Query: 373 LQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGTGMGE--ILAKLTTLS 432
           L++LV+SP+RSLSD I+FN  +  LE++  YGQ     A AT G G  E   LA+L  LS
Sbjct: 365 LKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARLAKLS 424

Query: 433 KEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTRKPFGFYFGGLKGYEP 492
           KEGFE+ MIKN+LDAI +PG LI PFLAIGGFPG+SVPAGY+P   PFG  FGGLKG++P
Sbjct: 425 KEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLKGFDP 484

Query: 493 RLIEIAYAFEQATKRRKPPRLSK 512
           RLIEIAY FE  T  RK P L +
Sbjct: 485 RLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of Tan0010682 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 442.6 bits (1137), Expect = 4.5e-124
Identity = 258/507 (50.89%), Postives = 328/507 (64.69%), Query Frame = 0

Query: 7   SLYISLLLILVSLFPFGSPSE---GTTFSIEEATLKDLQLAFNQNKLTSRQLVEFYIEQI 66
           SL I L+L    +   GS S+    +TFSI+EAT++D+++AFN+ +LTS+QLVE Y+E I
Sbjct: 11  SLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAI 70

Query: 67  RKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLVKDNIATKDKLRTT 126
            K N  L  VIE NPDA   A+ ADRER    T + LP+LHG+PVL+KD+I+TKDKL TT
Sbjct: 71  SKLNPILHAVIETNPDALIQAEIADRERDLKNT-TKLPILHGVPVLLKDSISTKDKLNTT 130

Query: 127 AGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPTGWSARNGRGYEAY 186
           AGSFALL S+V RDAGVV +LR++GA+ILGKASLS+W+ FRS   P GWSA         
Sbjct: 131 AGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA--------- 190

Query: 187 TFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKPTVGLTSRAGVVPI 246
                                                S NSVV IKP+VGLTSRAGVVPI
Sbjct: 191 ------------------------------------PSQNSVVGIKPSVGLTSRAGVVPI 250

Query: 247 SRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYGQFLKPDGLRGKRL 306
           S RQD++GP+ RTV+DA ++L+AIVGYD  D+AT   S++IP GGY QFL   GL+GKRL
Sbjct: 251 SLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKRL 310

Query: 307 GIVQDLSLGVDPSVAQAFQDVFPILRGKGAILVNNLTIKSFVTIANPFESGEWTALLNEF 366
           GIV   S  +D  +          LR +GAI++NNLTI +   I    +SGE  ALL EF
Sbjct: 311 GIVMKHSSLLDHHI--------KTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEF 370

Query: 367 RESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEVNTYGQGYLEAANATNGTGMGE--IL 426
           + SLNAYL+ELV SPVRSL+DVI +N    + E+V  +GQ     A AT+G G  E   L
Sbjct: 371 KMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTAL 430

Query: 427 AKLTTLSKEGFEKTMIKNELDAIISPGELIFPFLAIGGFPGISVPAGYSPTRKPFGFYFG 486
            K+  LS+ G EK + +N+LDAI++ G  +   LAIGG+PGI+VPAGY     P+G  FG
Sbjct: 431 QKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFG 463

Query: 487 GLKGYEPRLIEIAYAFEQATKRRKPPR 509
           GL+  EP+LIEIA+AFEQAT  RKPP+
Sbjct: 491 GLRFSEPKLIEIAFAFEQATLIRKPPK 463

BLAST of Tan0010682 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 123.6 bits (309), Expect = 4.6e-28
Identity = 137/503 (27.24%), Postives = 228/503 (45.33%), Query Frame = 0

Query: 51  TSRQLVEFYIEQIRKYNGRLRGVIEVNPDARDLADNADRERKANKTPSALPLLHGIPVLV 110
           T+ ++ + Y+ +IR    +L+  + V+ +   L D  + +++  K     PL  G+ + V
Sbjct: 59  TAVEIAKSYLSRIRLTEPQLKCFLHVSENV--LKDAQEIDQRIAKGEELGPLA-GVLIGV 118

Query: 111 KDNIATKDKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQWSGFRSRLAPT 170
           KDNI T+  + +TA S  L +   P DA  V K+++ G I++GK ++ ++    +  A  
Sbjct: 119 KDNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFGMGSTTEASA 178

Query: 171 GWSARNGRGYEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSSYNSVVTIKP 230
                N           P GSS GSA +V+     V++G++T GS+  P+S+  VV +KP
Sbjct: 179 FQVTANPWDLSRV----PGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKP 238

Query: 231 TVGLTSRAGVVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFDQATNCCSQYIPSGGYG 290
           T G  SR G++  +   D +G  G TVADA  +L AI GYD FD  ++   Q +P     
Sbjct: 239 TYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSS--KQDVPE-FQS 298

Query: 291 QFLKPD-----GLRGKRLGIVQD-LSLGVDPSVAQAFQDVFPILRGKGAILVN------N 350
           QFL  D      L G ++GI+++ L  GVD  V  A Q+    L   G IL        +
Sbjct: 299 QFLSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASHLEALGCILTEVSLPSFS 358

Query: 351 LTIKSFVTIANPFESGEWTALLNEFRESLNAYLQELVSSPVRSLSDVIQFNNNHKDLEEV 410
           L + ++  IA+  ES    +  +  R       +EL  + +   S    F    K    +
Sbjct: 359 LGLPAYYVIASS-ESSSNLSRYDGVRYGNQVMAEEL--NKLYECSRGEGFGGEVKMRILM 418

Query: 411 NTY--GQGYLEAANATNGTGMGEILAKLTTLSKEGFEKTMIKNELDAIISP--------- 470
            TY    GY +A          +   ++ TL ++ F+  + +N  D +ISP         
Sbjct: 419 GTYALSAGYYDA--------YYKRAQQVRTLIRKDFKAALEQN--DILISPAAPSAAYKI 478

Query: 471 ------------GELIFPFLAIGGFPGISVPAGY---SPTRKPFGFYFGGLKGYEPRLIE 514
                       G+++   + + G P + +P G     P+  P G    G    E +L++
Sbjct: 479 GEKKDDPLAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLK 537

BLAST of Tan0010682 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 97.4 bits (241), Expect = 3.5e-20
Identity = 75/233 (32.19%), Postives = 116/233 (49.79%), Query Frame = 0

Query: 49  KLTSRQLVEFYIEQIRKYNGRLRGVIEVNPD-ARDLADNADRERKANKTPSALPLLHGIP 108
           ++TS++LV  Y++Q+++YN  L  V+    + A   A  AD         + L  LHGIP
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD---DLLSQGTYLGPLHGIP 275

Query: 109 VLVKDNIATKDKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQ------WS 168
             +KD +A     +TT GS +  +  +  +A V  +L+ +GA+++ K           W 
Sbjct: 276 YGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWF 335

Query: 169 GFRSRLAPTGWSARNGRGYEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSS 228
           G R+         RN    E ++     GSS+G A S S  M    +G+ET GS+  P++
Sbjct: 336 GGRT---------RNPWNIEEFS----TGSSAGPAASTSAGMVPFAIGSETAGSMTYPAA 395

Query: 229 YNSVVTIKPTVGLTSRAGVVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFD 275
              +  ++PT G   R GV+ IS   D +GP  RT AD A +L+AI G D  D
Sbjct: 396 RCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 431

BLAST of Tan0010682 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 89.0 bits (219), Expect = 1.3e-17
Identity = 74/233 (31.76%), Postives = 114/233 (48.93%), Query Frame = 0

Query: 49  KLTSRQLVEFYIEQIRKYNGRLRGVIEVNPD-ARDLADNADRERKANKTPSALPLLHGIP 108
           ++TS++LV  Y++Q+++YN  L  V+    + A   A  AD         + L  LHGIP
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD---DLLSQGTYLGPLHGIP 275

Query: 109 VLVKDNIATKDKLRTTAGSFALLNSIVPRDAGVVTKLRKAGAIILGKASLSQ------WS 168
             +KD +A     +TT GS +  +  +  +A V  +L+ +GA+++ K           W 
Sbjct: 276 YGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWF 335

Query: 169 GFRSRLAPTGWSARNGRGYEAYTFGDPCGSSSGSAISVSTNMAAVTVGTETDGSLLCPSS 228
           G R+         RN    E ++     GSS+G A S S        G+ET GS+  P++
Sbjct: 336 GGRT---------RNPWNIEEFS----TGSSAGPAASTS-------AGSETAGSMTYPAA 395

Query: 229 YNSVVTIKPTVGLTSRAGVVPISRRQDTVGPMGRTVADAAYVLEAIVGYDVFD 275
              +  ++PT G   R GV+ IS   D +GP  RT AD A +L+AI G D  D
Sbjct: 396 RCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 424

BLAST of Tan0010682 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 63.2 bits (152), Expect = 7.4e-10
Identity = 49/165 (29.70%), Postives = 80/165 (48.48%), Query Frame = 0

Query: 96  TPSALPLLHGIPVLVKDNIATKDKLRTTAG-SFALLNSIVPRDAGVVTKLRKAGAIILGK 155
           T S+ P L G+   +KD    + ++       +   +S     A VV+ L +AGA  LG 
Sbjct: 21  TSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPVVSSLLEAGATALGI 80

Query: 156 ASLSQWSGFRSRLAPTGWSARNGRGYEAYTFGD-PCGSSSGSAISVSTNMAAVTVGTETD 215
             + + +      +  G +A  G       F   P GSSSGSA++V+  +   ++GT+T 
Sbjct: 81  TIMDEMA-----YSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLVDFSIGTDTG 140

Query: 216 GSLLCPSSYNSVVTIKPTVGLTSRAGVVPISRRQDTVGPMGRTVA 259
           GS+  P+SY  +   +P+ G  S  G+ P+++  DTVG   R  A
Sbjct: 141 GSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTA 180