Tan0010622 (gene) Snake gourd v1

BLAST of Tan0010622 vs. ExPASy Swiss-Prot
Match: Q9FKQ1 (Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 6.1e-197
Identity = 344/477 (72.12%), Postives = 401/477 (84.07%), Query Frame = 0

Query: 7   EHEQEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAF 66
           E   E+ V LL+S P   EE+   L  RI VE+KKLW IVGPAIFSRV +YSMLVITQAF
Sbjct: 7   EEGSESRVALLKS-PHTAEEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAF 66

Query: 67  AGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIV 126
           AGHLGDLELAA+SI NNV VGF+FGLLLGMASALETLCGQA+GAKK+HMLG+YMQRSWIV
Sbjct: 67  AGHLGDLELAAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIV 126

Query: 127 LFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQL 186
           LF CC+ LLP Y+F TP LK LGQP ++AEL+G VA+ ++PLHF+F   FPLQRFLQ QL
Sbjct: 127 LFFCCVLLLPTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQL 186

Query: 187 KTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPL 246
           K  V AY + VAL+VH+LV WL V GLKLG+ GT  TI+ SWWV V  L +Y+  GGCPL
Sbjct: 187 KNRVTAYAAAVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPL 246

Query: 247 TWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTI 306
           TW+G S EA +GLW+F+KLSA+SGVMLCLENWYYRILI+MTGNL+NA++AVD+LS+CM I
Sbjct: 247 TWTGLSSEALTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAI 306

Query: 307 NGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQI 366
           NGWEMMIPLAFF G+GVRVANELGAGNGKGA+FAT V+V  S+IIGLFFW++IM+   QI
Sbjct: 307 NGWEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQI 366

Query: 367 ALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGL 426
           A IF+SS  VL  V KLS+LLAFT+LLNSVQP+LSGVAVGSGWQSYVAY+NLGCYY IG+
Sbjct: 367 AWIFSSSVAVLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGV 426

Query: 427 PLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKWA 484
           PLGFLMGWGF  GVMGIW GMIFGGTA+QT+IL  +T+RCDW+KEA+KAS  I KW+
Sbjct: 427 PLGFLMGWGFKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWS 482

BLAST of Tan0010622 vs. ExPASy Swiss-Prot
Match: Q1PDX9 (Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 6.0e-184
Identity = 318/473 (67.23%), Postives = 393/473 (83.09%), Query Frame = 0

Query: 10  QEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAFAGH 69
           +EA VPLL+     +E   G +   IW+E+KK+W+IVGP+IF+ +A+YS+L+ITQAFAGH
Sbjct: 12  EEATVPLLECHNAAEE--GGGMKREIWIETKKIWYIVGPSIFTGLATYSILIITQAFAGH 71

Query: 70  LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI 129
           LGDLELAA+SI NN  +GF++GLLLGMASALETLCGQA+GA++++MLG+YMQR WI+LF+
Sbjct: 72  LGDLELAAISIINNFTLGFNYGLLLGMASALETLCGQAFGAREYYMLGVYMQRYWIILFL 131

Query: 130 CCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA 189
           CCI LLP+YLFATP LK +GQ  ++AEL G +A+ ++P+HF+FAF FPL RFLQ QLK  
Sbjct: 132 CCILLLPMYLFATPILKFIGQSDDIAELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNK 191

Query: 190 VIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPLTWS 249
           VIA  + V+L VH+LV W  VYG KLG+ GT  ++N  WW+ +F LF+Y+  GGC LTW+
Sbjct: 192 VIAISAGVSLAVHILVCWFFVYGYKLGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWT 251

Query: 250 GFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTINGW 309
           GFS EAF+GL +  KLSA+SG+MLCLENWYY+IL++MTGNL NAK+AVD+LS+CM++NGW
Sbjct: 252 GFSSEAFTGLLELTKLSASSGIMLCLENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGW 311

Query: 310 EMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQIALI 369
           EMMIPLAFF G+GVRVANELGAGNGKGA+FAT V++  S++IGLFF +II+IF  QI  I
Sbjct: 312 EMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSI 371

Query: 370 FTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPLG 429
           F+SSE VL  V  LSVLLAFT+LLNSVQP+LSGVAVGSGWQSYVAY+NLGCYYLIGLP G
Sbjct: 372 FSSSEAVLNAVDNLSVLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFG 431

Query: 430 FLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKW 483
             MGW F  GV GIWAGMIFGGTAIQTLIL I+T RCDWD EA K+S+ I+KW
Sbjct: 432 LTMGWIFKFGVKGIWAGMIFGGTAIQTLILIIITTRCDWDNEAHKSSVRIKKW 482

BLAST of Tan0010622 vs. ExPASy Swiss-Prot
Match: Q9FNC1 (Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 2.1e-173
Identity = 301/474 (63.50%), Postives = 383/474 (80.80%), Query Frame = 0

Query: 10  QEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAFAGH 69
           ++A +PLL+ +  + EE NG +   IW+E+KKLW IVGPAIF+RV +  + VITQAFAGH
Sbjct: 13  EKAKIPLLKDQ-NVAEEENGEIKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGH 72

Query: 70  LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI 129
           LG+LELAA+SI NNVI+GF++ L +GMA+ALETLCGQA+GAKK+ M G+Y+QRSWIVLF+
Sbjct: 73  LGELELAAISIVNNVIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFL 132

Query: 130 CCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA 189
             I LLP+Y+FATP LK +GQP ++AEL+G ++V  +P HFSFAF FP+ RFLQ QLK +
Sbjct: 133 FSILLLPMYIFATPILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNS 192

Query: 190 VIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPLTWS 249
           VIA  S V+L+VH+ V WL VY L+LG+ GT  T N SWW+ VF LF YT  GGCPLTW+
Sbjct: 193 VIAISSGVSLVVHIFVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWT 252

Query: 250 GFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTINGW 309
           GFS+E+F+ LW+F KLSA+SG+M+CLENWYYR+LIVMTGNLE+A++ VD++S+CM+ING 
Sbjct: 253 GFSMESFTRLWEFTKLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSINGL 312

Query: 310 EMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQIALI 369
           EMM+PLAFF G+ VRVANELGAGNGK A+FA  ++V  S+IIG+   ++I     QI  +
Sbjct: 313 EMMVPLAFFAGTSVRVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWM 372

Query: 370 FTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPLG 429
           F+SSE VLK V  LS+LL+F ILLNSVQP+LSGVAVGSGWQS VA++NLGCYY IGLPLG
Sbjct: 373 FSSSETVLKAVNNLSILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLG 432

Query: 430 FLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKWA 484
            +MGW F  GV GIWAGMIFGGT +QTLIL  +T+RCDW+KEA+ A + + KW+
Sbjct: 433 IVMGWMFKFGVKGIWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

BLAST of Tan0010622 vs. ExPASy Swiss-Prot
Match: Q8W488 (Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 4.8e-125
Identity = 231/459 (50.33%), Postives = 321/459 (69.93%), Query Frame = 0

Query: 24  KEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAFAGHLGDLELAAMSIANN 83
           +E++   L  ++W+ESKKLW +  PAIF+R +++ + +I+Q+F GHLG +ELAA SI   
Sbjct: 22  EEKDELGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIGHLGPIELAAYSITFT 81

Query: 84  VIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFICCIFLLPVYLFATP 143
           V++ F  G+LLGMASALETLCGQAYGAK+ HMLGIY+QRSWIVL  C I L PVY+F+ P
Sbjct: 82  VLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRSWIVLTGCTICLTPVYIFSGP 141

Query: 144 ALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTAVIAYVSFVALLVHV 203
            L  LGQ   +  +A  +A+ ++ ++FSF   F  Q FLQ+Q K  +IAYV+ V+L VHV
Sbjct: 142 ILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQAQSKNKIIAYVAAVSLGVHV 201

Query: 204 LVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPLTWSGFSVEAFSGLWDFV 263
            +SWLL+     G+ G   +   ++W+      ++   GGC  TW GFS+ AF  LW   
Sbjct: 202 FLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGGCKDTWRGFSMMAFKDLWPVF 261

Query: 264 KLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTINGWEMMIPLAFFVGSGV 323
           KLS +SG MLCLE WY  IL+++TGNL+NA++A+DAL++C+ ING EMMI L F   + V
Sbjct: 262 KLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAICLNINGLEMMIALGFLAAASV 321

Query: 324 RVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQIALIFTSSEIVLKEVKKL 383
           RV+NELG+GN KGAKFAT  AV TS+ +G+  + + +    +++ IFT+SE V  EV  L
Sbjct: 322 RVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSYIFTTSEAVAAEVADL 381

Query: 384 SVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPLGFLMGWGFNQGVMGI 443
           S LLAF+IL+NSVQP+LSGVAVG+GWQ YV YVNL CYYL+G+P+G ++G+     V G+
Sbjct: 382 SPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPIGIILGYVVGLQVKGV 441

Query: 444 WAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKW 483
           W GM+F G  +QT +L +MT+R DWD++   +   + +W
Sbjct: 442 WIGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRW 479

BLAST of Tan0010622 vs. ExPASy Swiss-Prot
Match: Q9SX83 (Protein DETOXIFICATION 33 OS=Arabidopsis thaliana OX=3702 GN=DTX33 PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.2e-123
Identity = 235/485 (48.45%), Postives = 329/485 (67.84%), Query Frame = 0

Query: 10  QEANVPLLQSKPQIKEENNGALS-TRIWV-----ESKKLWHIVGPAIFSRVASYSMLVIT 69
           ++  +PLL   P+   E  G  S +++W      ESK+LW + GPAIF+ ++ YS+  +T
Sbjct: 3   KDKTLPLLD--PREPPELTGTKSASKVWAKEFGEESKRLWELAGPAIFTAISQYSLGALT 62

Query: 70  QAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRS 129
           Q F+G LG+LELAA+S+ N+VI G  FG++LGM SALETLCGQAYGA +  M+GIYMQRS
Sbjct: 63  QTFSGRLGELELAAVSVENSVISGLAFGVMLGMGSALETLCGQAYGAGQIRMMGIYMQRS 122

Query: 130 WIVLFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQ 189
           W++LF   +FLLPVY++A P L   G+   +++ AGK A+ ++P  F++A  FP+Q+FLQ
Sbjct: 123 WVILFTTALFLLPVYIWAPPILSFFGEAPHISKAAGKFALWMIPQLFAYAANFPIQKFLQ 182

Query: 190 SQLKTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGG 249
           SQ K  V+A++S V L++H + SWL +   K GL G AIT+N SWW++V G  +Y +   
Sbjct: 183 SQRKVLVMAWISGVVLVIHAVFSWLFILYFKWGLVGAAITLNTSWWLIVIGQLLYILITK 242

Query: 250 CPLTWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVC 309
               W+GFS+ AF  L+ FVKLS AS +MLCLE WY  +L+V+TG L N  + VDA+S+C
Sbjct: 243 SDGAWTGFSMLAFRDLYGFVKLSLASALMLCLEFWYLMVLVVVTGLLPNPLIPVDAISIC 302

Query: 310 MTINGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFD 369
           M I GW  MI + F     VRV+NELGAGN   AKF+  V   TS +IG+   I+++   
Sbjct: 303 MNIEGWTAMISIGFNAAISVRVSNELGAGNAALAKFSVIVVSITSTLIGIVCMIVVLATK 362

Query: 370 TQIALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYL 429
                +FTSSE V  E  +++VLL FT+LLNS+QP+LSGVAVG+GWQ+ VAYVN+ CYY+
Sbjct: 363 DSFPYLFTSSEAVAAETTRIAVLLGFTVLLNSLQPVLSGVAVGAGWQALVAYVNIACYYI 422

Query: 430 IGLPLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKWA 489
           IGLP G ++G+  + GV GIW GM+  G  +QTLIL  +    +W+KEAE+A   +++W 
Sbjct: 423 IGLPAGLVLGFTLDLGVQGIWGGMV-AGICLQTLILIGIIYFTNWNKEAEQAESRVQRWG 482

BLAST of Tan0010622 vs. NCBI nr
Match: KAG6600668.1 (Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 847.8 bits (2189), Expect = 4.6e-242
Identity = 437/486 (89.92%), Postives = 460/486 (94.65%), Query Frame = 0

Query: 1   MSTNIAEHEQEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSML 60
           MST+ A   QE NVPLLQSK  I+EE++  LSTRI VES+KLWHIVGPA+FSRVASYSML
Sbjct: 1   MSTHPA--AQEPNVPLLQSKSLIQEEDDAPLSTRIRVESRKLWHIVGPAVFSRVASYSML 60

Query: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120
           VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM
Sbjct: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120

Query: 121 QRSWIVLFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQR 180
           QRSWIVLFICCIFLLP+YLFATPALKLLGQP++LAELAGKVAVLLVPLHFSFAFQFPLQR
Sbjct: 121 QRSWIVLFICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQR 180

Query: 181 FLQSQLKTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTV 240
           FLQSQLKTAVIAYVS VALLVH+LVSW  V GLKLGLFGTAITIN SWWVLVFGL IYTV
Sbjct: 181 FLQSQLKTAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTV 240

Query: 241 SGGCPLTWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDAL 300
           SGGCP TW+GFS+E FSGLWDFVKLSAASG+MLCLENWYYRILIVMTGNL+NAK+AVDAL
Sbjct: 241 SGGCPHTWTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDAL 300

Query: 301 SVCMTINGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIM 360
           SVCMTINGWEMMIPLAFF GSGVRVANELGAGNGKGAKFATKVAVGTSV+IG+FFWIIIM
Sbjct: 301 SVCMTINGWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIM 360

Query: 361 IFDTQIALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGC 420
            FDTQI+LIF+SSE+VLKEVKKLS+LLAFTILLNSVQP+LSGVAVGSGWQSYVAYVNLGC
Sbjct: 361 TFDTQISLIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGC 420

Query: 421 YYLIGLPLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIR 480
           YYLIGLPLG LMGWGF QGVMGIW GMIFGGTAIQTLILCIMTIRCDWDKEAEKA L IR
Sbjct: 421 YYLIGLPLGLLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEAEKARLQIR 480

Query: 481 KWAEES 487
           +W EES
Sbjct: 481 EWGEES 484

BLAST of Tan0010622 vs. NCBI nr
Match: XP_023516283.1 (protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845.5 bits (2183), Expect = 2.3e-241
Identity = 435/486 (89.51%), Postives = 460/486 (94.65%), Query Frame = 0

Query: 1   MSTNIAEHEQEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSML 60
           MST+ A   QE NVPLLQSK  I+EE++  LSTRI VES+KLWHIVGPA+FSRVASYSML
Sbjct: 1   MSTHPA--AQEPNVPLLQSKSLIQEEDDAPLSTRIRVESRKLWHIVGPAVFSRVASYSML 60

Query: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120
           VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM
Sbjct: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120

Query: 121 QRSWIVLFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQR 180
           QRSWIVLFICCIFLLP+YLFATPALKLLGQP++LAELAGKVAVLLVPLHFSFAFQFPLQR
Sbjct: 121 QRSWIVLFICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQR 180

Query: 181 FLQSQLKTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTV 240
           FLQSQLKTAVIAYVS VALLVH+LVSW  V GLKLGLFGTAITIN SWWVLVFGL IYTV
Sbjct: 181 FLQSQLKTAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTV 240

Query: 241 SGGCPLTWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDAL 300
           SGGCP TW+GFS+E FSGLWDFVKLSAASG+MLCLENWYYRILIVMTGNL+NAK+AVDAL
Sbjct: 241 SGGCPHTWTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDAL 300

Query: 301 SVCMTINGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIM 360
           SVCMTINGWEMMIPLAFF GSGVRVANELGAGNGKGAKFATKVAVGTS++IG+FFWIIIM
Sbjct: 301 SVCMTINGWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSMVIGVFFWIIIM 360

Query: 361 IFDTQIALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGC 420
            FDTQI+LIF+SSE+VLKEVKKLS+LLAFTILLNSVQP+LSGVAVGSGWQSYVAYVNLGC
Sbjct: 361 TFDTQISLIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGC 420

Query: 421 YYLIGLPLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIR 480
           YYLIGLPLG LMGWGF QGVMGIW GMIFGGTAIQTLILCIMTIRCDWDKEAEKA L IR
Sbjct: 421 YYLIGLPLGLLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEAEKARLQIR 480

Query: 481 KWAEES 487
           +W EE+
Sbjct: 481 EWGEET 484

BLAST of Tan0010622 vs. NCBI nr
Match: XP_022941750.1 (protein DETOXIFICATION 27-like [Cucurbita moschata])

HSP 1 Score: 844.7 bits (2181), Expect = 3.9e-241
Identity = 431/477 (90.36%), Postives = 455/477 (95.39%), Query Frame = 0

Query: 10  QEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAFAGH 69
           QE NVPLLQSK  I+EE++  LSTRI VES+KLWHIVGPA+FSRVASYSMLVITQAFAGH
Sbjct: 8   QEPNVPLLQSKSLIQEEDDAPLSTRIRVESRKLWHIVGPAVFSRVASYSMLVITQAFAGH 67

Query: 70  LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI 129
           LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI
Sbjct: 68  LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI 127

Query: 130 CCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA 189
           CCIFLLP+YLFATPALKLLGQP++LAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA
Sbjct: 128 CCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA 187

Query: 190 VIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPLTWS 249
           VIAYVS VALLVH+LVSW  V GLKLGLFGTAIT+N SWWVLVFGL IYTVSGGCP TW+
Sbjct: 188 VIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITMNISWWVLVFGLLIYTVSGGCPHTWT 247

Query: 250 GFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTINGW 309
           GFS+E FSGLWDFVKLSAASG+MLCLENWYYRILIVMTGNL+NAK+AVDALSVCMTINGW
Sbjct: 248 GFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDALSVCMTINGW 307

Query: 310 EMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQIALI 369
           EMMIPLAFF GSGVRVANELGAGNGKGAKFATKVAVGTSV+IG+FFWIIIM FDTQI+LI
Sbjct: 308 EMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIMTFDTQISLI 367

Query: 370 FTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPLG 429
           F+SSE+VLKEVKKLS+LLAFTILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLPLG
Sbjct: 368 FSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLPLG 427

Query: 430 FLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKWAEES 487
            LMGWGF QGVMGIW GMIFGGTAIQTLILCIMTIRCDWDKEAEKA L IR+W EE+
Sbjct: 428 LLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEAEKARLQIREWGEET 484

BLAST of Tan0010622 vs. NCBI nr
Match: XP_022999771.1 (protein DETOXIFICATION 27-like [Cucurbita maxima])

HSP 1 Score: 844.0 bits (2179), Expect = 6.7e-241
Identity = 434/489 (88.75%), Postives = 462/489 (94.48%), Query Frame = 0

Query: 1   MSTNIAEHEQEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSML 60
           MST+ A   QE NVPLLQSK  I+EE++  LSTRI VES+KLWHIVGPA+FSRVASYSML
Sbjct: 1   MSTHPA--AQEPNVPLLQSKSLIQEEDDAPLSTRIRVESRKLWHIVGPAVFSRVASYSML 60

Query: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120
           VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM
Sbjct: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120

Query: 121 QRSWIVLFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQR 180
           QRSWIVLFICCIFLLP+YLFATPALKLLGQP++LAELAGKVAVLLVPLHFSFAFQFPLQR
Sbjct: 121 QRSWIVLFICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQR 180

Query: 181 FLQSQLKTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTV 240
           FLQSQLKTAVIAYVS VALLVH+LVSW  V GLKLGLFGTAITIN SWWVLVFGL IYTV
Sbjct: 181 FLQSQLKTAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTV 240

Query: 241 SGGCPLTWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDAL 300
           SGGCP TW+GFS+E FSGLWDFVKLSAASG+MLCLENWYYRILIVMTGNL+NAK+AVDAL
Sbjct: 241 SGGCPHTWTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDAL 300

Query: 301 SVCMTINGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIM 360
           SVCMTINGWEMMIPLAFF GSGVRVANELGAGNGKGAKFATKVAVGTSV+IG+FFWIIIM
Sbjct: 301 SVCMTINGWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIM 360

Query: 361 IFDTQIALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGC 420
            FDTQI+LIF+SSE+VLKEVKKLS+LLAFTILLNSVQP+LSGVAVGSGWQSYVAYVNLGC
Sbjct: 361 TFDTQISLIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGC 420

Query: 421 YYLIGLPLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIR 480
           YYLIGLPLG LMGWGF QGVMGIW GMIFGGTAIQT+ILCI+TIRCDWDKEAEKA L IR
Sbjct: 421 YYLIGLPLGLLMGWGFKQGVMGIWGGMIFGGTAIQTMILCIITIRCDWDKEAEKARLQIR 480

Query: 481 KWAEESLEQ 490
           +W E++ +Q
Sbjct: 481 EWGEDTGDQ 487

BLAST of Tan0010622 vs. NCBI nr
Match: KAG7031307.1 (Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 840.5 bits (2170), Expect = 7.4e-240
Identity = 437/494 (88.46%), Postives = 460/494 (93.12%), Query Frame = 0

Query: 1   MSTNIAEHEQEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSML 60
           MST+ A   QE NVPLLQSK  I+EE++  LSTRI VES+KLWHIVGPA+FSRVASYSML
Sbjct: 1   MSTHPA--AQEPNVPLLQSKSLIQEEDDAPLSTRIRVESRKLWHIVGPAVFSRVASYSML 60

Query: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120
           VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM
Sbjct: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120

Query: 121 QRSWIVLFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQR 180
           QRSWIVLFICCIFLLP+YLFATPALKLLGQP++LAELAGKVAVLLVPLHFSFAFQFPLQR
Sbjct: 121 QRSWIVLFICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQR 180

Query: 181 FLQSQLKTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTV 240
           FLQSQLKTAVIAYVS VALLVH+LVSW  V GLKLGLFGTAITIN SWWVLVFGL IYTV
Sbjct: 181 FLQSQLKTAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTV 240

Query: 241 SGGCPLTWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDAL 300
           SGGCP TW+GFS+E FSGLWDFVKLSAASG+MLCLENWYYRILIVMTGNL+NAK+AVDAL
Sbjct: 241 SGGCPHTWTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDAL 300

Query: 301 SVCMTINGWEMMIPLAFFVGSG--------VRVANELGAGNGKGAKFATKVAVGTSVIIG 360
           SVCMTINGWEMMIPLAFF GSG        VRVANELGAGNGKGAKFATKVAVGTSV+IG
Sbjct: 301 SVCMTINGWEMMIPLAFFAGSGFRIFDEYRVRVANELGAGNGKGAKFATKVAVGTSVVIG 360

Query: 361 LFFWIIIMIFDTQIALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSY 420
           +FFWIIIM FDTQI+LIF+SSE+VLKEVKKLS+LLAFTILLNSVQP+LSGVAVGSGWQSY
Sbjct: 361 VFFWIIIMTFDTQISLIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSY 420

Query: 421 VAYVNLGCYYLIGLPLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEA 480
           VAYVNLGCYYLIGLPLG LMGWGF QGVMGIW GMIFGGTAIQTLILCIMTIRCDWDKEA
Sbjct: 421 VAYVNLGCYYLIGLPLGLLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEA 480

Query: 481 EKASLHIRKWAEES 487
           EKA L IR+W EES
Sbjct: 481 EKARLQIREWGEES 492

BLAST of Tan0010622 vs. ExPASy TrEMBL
Match: A0A6J1FUM6 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111447024 PE=3 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.9e-241
Identity = 431/477 (90.36%), Postives = 455/477 (95.39%), Query Frame = 0

Query: 10  QEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAFAGH 69
           QE NVPLLQSK  I+EE++  LSTRI VES+KLWHIVGPA+FSRVASYSMLVITQAFAGH
Sbjct: 8   QEPNVPLLQSKSLIQEEDDAPLSTRIRVESRKLWHIVGPAVFSRVASYSMLVITQAFAGH 67

Query: 70  LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI 129
           LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI
Sbjct: 68  LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI 127

Query: 130 CCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA 189
           CCIFLLP+YLFATPALKLLGQP++LAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA
Sbjct: 128 CCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA 187

Query: 190 VIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPLTWS 249
           VIAYVS VALLVH+LVSW  V GLKLGLFGTAIT+N SWWVLVFGL IYTVSGGCP TW+
Sbjct: 188 VIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITMNISWWVLVFGLLIYTVSGGCPHTWT 247

Query: 250 GFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTINGW 309
           GFS+E FSGLWDFVKLSAASG+MLCLENWYYRILIVMTGNL+NAK+AVDALSVCMTINGW
Sbjct: 248 GFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDALSVCMTINGW 307

Query: 310 EMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQIALI 369
           EMMIPLAFF GSGVRVANELGAGNGKGAKFATKVAVGTSV+IG+FFWIIIM FDTQI+LI
Sbjct: 308 EMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIMTFDTQISLI 367

Query: 370 FTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPLG 429
           F+SSE+VLKEVKKLS+LLAFTILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLPLG
Sbjct: 368 FSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLPLG 427

Query: 430 FLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKWAEES 487
            LMGWGF QGVMGIW GMIFGGTAIQTLILCIMTIRCDWDKEAEKA L IR+W EE+
Sbjct: 428 LLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEAEKARLQIREWGEET 484

BLAST of Tan0010622 vs. ExPASy TrEMBL
Match: A0A6J1KE20 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111494018 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.2e-241
Identity = 434/489 (88.75%), Postives = 462/489 (94.48%), Query Frame = 0

Query: 1   MSTNIAEHEQEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSML 60
           MST+ A   QE NVPLLQSK  I+EE++  LSTRI VES+KLWHIVGPA+FSRVASYSML
Sbjct: 1   MSTHPA--AQEPNVPLLQSKSLIQEEDDAPLSTRIRVESRKLWHIVGPAVFSRVASYSML 60

Query: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120
           VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM
Sbjct: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120

Query: 121 QRSWIVLFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQR 180
           QRSWIVLFICCIFLLP+YLFATPALKLLGQP++LAELAGKVAVLLVPLHFSFAFQFPLQR
Sbjct: 121 QRSWIVLFICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQR 180

Query: 181 FLQSQLKTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTV 240
           FLQSQLKTAVIAYVS VALLVH+LVSW  V GLKLGLFGTAITIN SWWVLVFGL IYTV
Sbjct: 181 FLQSQLKTAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTV 240

Query: 241 SGGCPLTWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDAL 300
           SGGCP TW+GFS+E FSGLWDFVKLSAASG+MLCLENWYYRILIVMTGNL+NAK+AVDAL
Sbjct: 241 SGGCPHTWTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDAL 300

Query: 301 SVCMTINGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIM 360
           SVCMTINGWEMMIPLAFF GSGVRVANELGAGNGKGAKFATKVAVGTSV+IG+FFWIIIM
Sbjct: 301 SVCMTINGWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIM 360

Query: 361 IFDTQIALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGC 420
            FDTQI+LIF+SSE+VLKEVKKLS+LLAFTILLNSVQP+LSGVAVGSGWQSYVAYVNLGC
Sbjct: 361 TFDTQISLIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGC 420

Query: 421 YYLIGLPLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIR 480
           YYLIGLPLG LMGWGF QGVMGIW GMIFGGTAIQT+ILCI+TIRCDWDKEAEKA L IR
Sbjct: 421 YYLIGLPLGLLMGWGFKQGVMGIWGGMIFGGTAIQTMILCIITIRCDWDKEAEKARLQIR 480

Query: 481 KWAEESLEQ 490
           +W E++ +Q
Sbjct: 481 EWGEDTGDQ 487

BLAST of Tan0010622 vs. ExPASy TrEMBL
Match: A0A6J1ESE5 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111436076 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 2.8e-237
Identity = 427/489 (87.32%), Postives = 454/489 (92.84%), Query Frame = 0

Query: 1   MSTNIAEHEQEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSML 60
           M+TNI   EQE+ VPLLQSKP  K   NGAL TRIWVES+KLWHIVGPAIFSR+ASYSML
Sbjct: 1   MATNII--EQESTVPLLQSKP--KHNGNGALPTRIWVESQKLWHIVGPAIFSRLASYSML 60

Query: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120
           VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM
Sbjct: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120

Query: 121 QRSWIVLFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQR 180
           QRSWIVLFIC + +LP+YLFATPAL LLGQP +LAELAGKVA LLVPLHFSFA QFP+QR
Sbjct: 121 QRSWIVLFICSVLILPIYLFATPALLLLGQPPDLAELAGKVAALLVPLHFSFAIQFPVQR 180

Query: 181 FLQSQLKTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTV 240
           FLQSQLKTAVIAYVS VAL+VHVLVSWLLVY L+LGL GTAIT N SWWVLV GLF Y V
Sbjct: 181 FLQSQLKTAVIAYVSLVALVVHVLVSWLLVYNLELGLVGTAITTNISWWVLVLGLFFYAV 240

Query: 241 SGGCPLTWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDAL 300
            GGCPLTW GFSVEAFSGLW+FVKLSAASG+MLCLENWYYRILIVMTGNLENAKLAVDAL
Sbjct: 241 CGGCPLTWPGFSVEAFSGLWEFVKLSAASGIMLCLENWYYRILIVMTGNLENAKLAVDAL 300

Query: 301 SVCMTINGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIM 360
           SVCMTINGWEMMIP AFF GSGVRVANELGAGNGKGA+FAT VAVGTSV+IGLFFWIII+
Sbjct: 301 SVCMTINGWEMMIPFAFFAGSGVRVANELGAGNGKGARFATMVAVGTSVVIGLFFWIIII 360

Query: 361 IFDTQIALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGC 420
            FDTQIALIFTSS++VLKEV KLS+LLAFTILLNS+QP+LSGVAVGSGWQSYVAYVNLGC
Sbjct: 361 TFDTQIALIFTSSQVVLKEVNKLSILLAFTILLNSIQPVLSGVAVGSGWQSYVAYVNLGC 420

Query: 421 YYLIGLPLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIR 480
           YYLIGLPLG +MGWGFNQGVMGIWAGMIFGGTAIQTL+LCIMTIRCDWD+EAE+ SLHIR
Sbjct: 421 YYLIGLPLGIVMGWGFNQGVMGIWAGMIFGGTAIQTLVLCIMTIRCDWDREAERTSLHIR 480

Query: 481 KWAEESLEQ 490
           K AEE+ +Q
Sbjct: 481 KLAEETQDQ 485

BLAST of Tan0010622 vs. ExPASy TrEMBL
Match: A0A6J1J2H5 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111482817 PE=3 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 3.5e-235
Identity = 423/489 (86.50%), Postives = 452/489 (92.43%), Query Frame = 0

Query: 1   MSTNIAEHEQEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSML 60
           M+TNI   EQE+ VPLLQSKP+  +  NGAL TRIWVES+KLWHIVGPAIFSR+ASYSML
Sbjct: 1   MATNII--EQESTVPLLQSKPE--DNGNGALPTRIWVESQKLWHIVGPAIFSRLASYSML 60

Query: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120
           VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM
Sbjct: 61  VITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYM 120

Query: 121 QRSWIVLFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQR 180
           QRSWIVLFIC + +LP+YLFATPAL LLGQP +LAELAGKVA LLVPLHFSFA QFP+QR
Sbjct: 121 QRSWIVLFICSVLILPIYLFATPALLLLGQPPDLAELAGKVAALLVPLHFSFAIQFPVQR 180

Query: 181 FLQSQLKTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTV 240
           FLQSQLKTAVIAYVS VAL+VHVLVSWLLVY L+LGL GTAIT N SWWVLV GLF Y V
Sbjct: 181 FLQSQLKTAVIAYVSLVALVVHVLVSWLLVYNLELGLVGTAITTNISWWVLVLGLFFYAV 240

Query: 241 SGGCPLTWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDAL 300
            GGCPLTW GFSVEAFSGLW+FVKLSAASG+MLCLENWYYRILIVMTGNLENAKLAVDAL
Sbjct: 241 CGGCPLTWPGFSVEAFSGLWEFVKLSAASGIMLCLENWYYRILIVMTGNLENAKLAVDAL 300

Query: 301 SVCMTINGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIM 360
           SVCMTINGWEMMIP AFF GSGVRVANELG GNGKGA+FAT VAVGTS+IIGLFFWIII+
Sbjct: 301 SVCMTINGWEMMIPFAFFAGSGVRVANELGDGNGKGARFATMVAVGTSIIIGLFFWIIII 360

Query: 361 IFDTQIALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGC 420
            FDTQIALIFTSS++VLKEV KLS+LLAFTILLNS+QP+LSGVAVGSGWQSYVAYVNLGC
Sbjct: 361 TFDTQIALIFTSSQVVLKEVNKLSILLAFTILLNSIQPVLSGVAVGSGWQSYVAYVNLGC 420

Query: 421 YYLIGLPLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIR 480
           YYLIGLP G +MGW FNQGVMGIWAGMIFGGTAIQTL+LCIMTIRCDWD+EAE+ SLHIR
Sbjct: 421 YYLIGLPFGIVMGWSFNQGVMGIWAGMIFGGTAIQTLVLCIMTIRCDWDREAERTSLHIR 480

Query: 481 KWAEESLEQ 490
           K AEE+ E+
Sbjct: 481 KLAEETQER 485

BLAST of Tan0010622 vs. ExPASy TrEMBL
Match: A0A0A0KZ65 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_4G099750 PE=3 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.5e-233
Identity = 416/479 (86.85%), Postives = 447/479 (93.32%), Query Frame = 0

Query: 11  EANVPLLQSKPQIKEE---NNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAFA 70
           E+NVPLL+ KPQI EE   N  +LSTRIWVESK+LWHIVGPAIFSRVASYSMLVITQAFA
Sbjct: 3   ESNVPLLELKPQIIEEDDANGSSLSTRIWVESKQLWHIVGPAIFSRVASYSMLVITQAFA 62

Query: 71  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 130
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL
Sbjct: 63  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 122

Query: 131 FICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLK 190
           FIC I +LP+YLF+TP LKLLGQPS+LAE+AGKVA++ +PLHFSFA QFPLQRFLQSQLK
Sbjct: 123 FICSILILPIYLFSTPVLKLLGQPSDLAEMAGKVAMVFLPLHFSFAIQFPLQRFLQSQLK 182

Query: 191 TAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPLT 250
           TAVIAY+S VAL+VH+LVSWL VYGLKLGL GTAIT N SWWVLVFGL  YT+ GGCP T
Sbjct: 183 TAVIAYLSLVALVVHILVSWLFVYGLKLGLVGTAITTNISWWVLVFGLLFYTICGGCPGT 242

Query: 251 WSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTIN 310
           W GFS EAFSGLW+FVKLS ASGVM+CLENWYYRILIVMTGNL NAKLAVDALSVCMTIN
Sbjct: 243 WCGFSFEAFSGLWEFVKLSTASGVMICLENWYYRILIVMTGNLANAKLAVDALSVCMTIN 302

Query: 311 GWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQIA 370
           GWEMMIPLAFFVGSGVRVANELGAGNGKGAKFAT VAVGTS+IIG+FFWIII+ FD+QIA
Sbjct: 303 GWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATMVAVGTSIIIGIFFWIIIITFDSQIA 362

Query: 371 LIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 430
           LIFTSSE+VLKEVK L++LLAFTILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLP
Sbjct: 363 LIFTSSEVVLKEVKTLTILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLP 422

Query: 431 LGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKWAEES 487
           LGFLMGWGFN GV GIWAGMIFGGTAIQTLILCIMTIRCDW+KEAE+AS+ I+KW EE+
Sbjct: 423 LGFLMGWGFNLGVKGIWAGMIFGGTAIQTLILCIMTIRCDWNKEAERASVQIKKWEEET 481

BLAST of Tan0010622 vs. TAIR 10
Match: AT5G65380.1 (MATE efflux family protein )

HSP 1 Score: 688.3 bits (1775), Expect = 4.4e-198
Identity = 344/477 (72.12%), Postives = 401/477 (84.07%), Query Frame = 0

Query: 7   EHEQEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAF 66
           E   E+ V LL+S P   EE+   L  RI VE+KKLW IVGPAIFSRV +YSMLVITQAF
Sbjct: 7   EEGSESRVALLKS-PHTAEEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAF 66

Query: 67  AGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIV 126
           AGHLGDLELAA+SI NNV VGF+FGLLLGMASALETLCGQA+GAKK+HMLG+YMQRSWIV
Sbjct: 67  AGHLGDLELAAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIV 126

Query: 127 LFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQL 186
           LF CC+ LLP Y+F TP LK LGQP ++AEL+G VA+ ++PLHF+F   FPLQRFLQ QL
Sbjct: 127 LFFCCVLLLPTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQL 186

Query: 187 KTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPL 246
           K  V AY + VAL+VH+LV WL V GLKLG+ GT  TI+ SWWV V  L +Y+  GGCPL
Sbjct: 187 KNRVTAYAAAVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPL 246

Query: 247 TWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTI 306
           TW+G S EA +GLW+F+KLSA+SGVMLCLENWYYRILI+MTGNL+NA++AVD+LS+CM I
Sbjct: 247 TWTGLSSEALTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAI 306

Query: 307 NGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQI 366
           NGWEMMIPLAFF G+GVRVANELGAGNGKGA+FAT V+V  S+IIGLFFW++IM+   QI
Sbjct: 307 NGWEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQI 366

Query: 367 ALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGL 426
           A IF+SS  VL  V KLS+LLAFT+LLNSVQP+LSGVAVGSGWQSYVAY+NLGCYY IG+
Sbjct: 367 AWIFSSSVAVLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGV 426

Query: 427 PLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKWA 484
           PLGFLMGWGF  GVMGIW GMIFGGTA+QT+IL  +T+RCDW+KEA+KAS  I KW+
Sbjct: 427 PLGFLMGWGFKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWS 482

BLAST of Tan0010622 vs. TAIR 10
Match: AT5G10420.1 (MATE efflux family protein )

HSP 1 Score: 645.2 bits (1663), Expect = 4.2e-185
Identity = 318/473 (67.23%), Postives = 393/473 (83.09%), Query Frame = 0

Query: 10  QEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAFAGH 69
           +EA VPLL+     +E   G +   IW+E+KK+W+IVGP+IF+ +A+YS+L+ITQAFAGH
Sbjct: 12  EEATVPLLECHNAAEE--GGGMKREIWIETKKIWYIVGPSIFTGLATYSILIITQAFAGH 71

Query: 70  LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI 129
           LGDLELAA+SI NN  +GF++GLLLGMASALETLCGQA+GA++++MLG+YMQR WI+LF+
Sbjct: 72  LGDLELAAISIINNFTLGFNYGLLLGMASALETLCGQAFGAREYYMLGVYMQRYWIILFL 131

Query: 130 CCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA 189
           CCI LLP+YLFATP LK +GQ  ++AEL G +A+ ++P+HF+FAF FPL RFLQ QLK  
Sbjct: 132 CCILLLPMYLFATPILKFIGQSDDIAELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNK 191

Query: 190 VIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPLTWS 249
           VIA  + V+L VH+LV W  VYG KLG+ GT  ++N  WW+ +F LF+Y+  GGC LTW+
Sbjct: 192 VIAISAGVSLAVHILVCWFFVYGYKLGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWT 251

Query: 250 GFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTINGW 309
           GFS EAF+GL +  KLSA+SG+MLCLENWYY+IL++MTGNL NAK+AVD+LS+CM++NGW
Sbjct: 252 GFSSEAFTGLLELTKLSASSGIMLCLENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGW 311

Query: 310 EMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQIALI 369
           EMMIPLAFF G+GVRVANELGAGNGKGA+FAT V++  S++IGLFF +II+IF  QI  I
Sbjct: 312 EMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSI 371

Query: 370 FTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPLG 429
           F+SSE VL  V  LSVLLAFT+LLNSVQP+LSGVAVGSGWQSYVAY+NLGCYYLIGLP G
Sbjct: 372 FSSSEAVLNAVDNLSVLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFG 431

Query: 430 FLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKW 483
             MGW F  GV GIWAGMIFGGTAIQTLIL I+T RCDWD EA K+S+ I+KW
Sbjct: 432 LTMGWIFKFGVKGIWAGMIFGGTAIQTLILIIITTRCDWDNEAHKSSVRIKKW 482

BLAST of Tan0010622 vs. TAIR 10
Match: AT5G44050.1 (MATE efflux family protein )

HSP 1 Score: 610.1 bits (1572), Expect = 1.5e-174
Identity = 301/474 (63.50%), Postives = 383/474 (80.80%), Query Frame = 0

Query: 10  QEANVPLLQSKPQIKEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAFAGH 69
           ++A +PLL+ +  + EE NG +   IW+E+KKLW IVGPAIF+RV +  + VITQAFAGH
Sbjct: 13  EKAKIPLLKDQ-NVAEEENGEIKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGH 72

Query: 70  LGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFI 129
           LG+LELAA+SI NNVI+GF++ L +GMA+ALETLCGQA+GAKK+ M G+Y+QRSWIVLF+
Sbjct: 73  LGELELAAISIVNNVIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFL 132

Query: 130 CCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTA 189
             I LLP+Y+FATP LK +GQP ++AEL+G ++V  +P HFSFAF FP+ RFLQ QLK +
Sbjct: 133 FSILLLPMYIFATPILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNS 192

Query: 190 VIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPLTWS 249
           VIA  S V+L+VH+ V WL VY L+LG+ GT  T N SWW+ VF LF YT  GGCPLTW+
Sbjct: 193 VIAISSGVSLVVHIFVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWT 252

Query: 250 GFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTINGW 309
           GFS+E+F+ LW+F KLSA+SG+M+CLENWYYR+LIVMTGNLE+A++ VD++S+CM+ING 
Sbjct: 253 GFSMESFTRLWEFTKLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSINGL 312

Query: 310 EMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQIALI 369
           EMM+PLAFF G+ VRVANELGAGNGK A+FA  ++V  S+IIG+   ++I     QI  +
Sbjct: 313 EMMVPLAFFAGTSVRVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWM 372

Query: 370 FTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPLG 429
           F+SSE VLK V  LS+LL+F ILLNSVQP+LSGVAVGSGWQS VA++NLGCYY IGLPLG
Sbjct: 373 FSSSETVLKAVNNLSILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLG 432

Query: 430 FLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKWA 484
            +MGW F  GV GIWAGMIFGGT +QTLIL  +T+RCDW+KEA+ A + + KW+
Sbjct: 433 IVMGWMFKFGVKGIWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

BLAST of Tan0010622 vs. TAIR 10
Match: AT1G33110.1 (MATE efflux family protein )

HSP 1 Score: 449.5 bits (1155), Expect = 3.4e-126
Identity = 231/459 (50.33%), Postives = 321/459 (69.93%), Query Frame = 0

Query: 24  KEENNGALSTRIWVESKKLWHIVGPAIFSRVASYSMLVITQAFAGHLGDLELAAMSIANN 83
           +E++   L  ++W+ESKKLW +  PAIF+R +++ + +I+Q+F GHLG +ELAA SI   
Sbjct: 22  EEKDELGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIGHLGPIELAAYSITFT 81

Query: 84  VIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVLFICCIFLLPVYLFATP 143
           V++ F  G+LLGMASALETLCGQAYGAK+ HMLGIY+QRSWIVL  C I L PVY+F+ P
Sbjct: 82  VLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRSWIVLTGCTICLTPVYIFSGP 141

Query: 144 ALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLKTAVIAYVSFVALLVHV 203
            L  LGQ   +  +A  +A+ ++ ++FSF   F  Q FLQ+Q K  +IAYV+ V+L VHV
Sbjct: 142 ILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQAQSKNKIIAYVAAVSLGVHV 201

Query: 204 LVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGGCPLTWSGFSVEAFSGLWDFV 263
            +SWLL+     G+ G   +   ++W+      ++   GGC  TW GFS+ AF  LW   
Sbjct: 202 FLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGGCKDTWRGFSMMAFKDLWPVF 261

Query: 264 KLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVCMTINGWEMMIPLAFFVGSGV 323
           KLS +SG MLCLE WY  IL+++TGNL+NA++A+DAL++C+ ING EMMI L F   + V
Sbjct: 262 KLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAICLNINGLEMMIALGFLAAASV 321

Query: 324 RVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFDTQIALIFTSSEIVLKEVKKL 383
           RV+NELG+GN KGAKFAT  AV TS+ +G+  + + +    +++ IFT+SE V  EV  L
Sbjct: 322 RVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSYIFTTSEAVAAEVADL 381

Query: 384 SVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPLGFLMGWGFNQGVMGI 443
           S LLAF+IL+NSVQP+LSGVAVG+GWQ YV YVNL CYYL+G+P+G ++G+     V G+
Sbjct: 382 SPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPIGIILGYVVGLQVKGV 441

Query: 444 WAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKW 483
           W GM+F G  +QT +L +MT+R DWD++   +   + +W
Sbjct: 442 WIGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRW 479

BLAST of Tan0010622 vs. TAIR 10
Match: AT1G47530.1 (MATE efflux family protein )

HSP 1 Score: 444.9 bits (1143), Expect = 8.4e-125
Identity = 235/485 (48.45%), Postives = 329/485 (67.84%), Query Frame = 0

Query: 10  QEANVPLLQSKPQIKEENNGALS-TRIWV-----ESKKLWHIVGPAIFSRVASYSMLVIT 69
           ++  +PLL   P+   E  G  S +++W      ESK+LW + GPAIF+ ++ YS+  +T
Sbjct: 3   KDKTLPLLD--PREPPELTGTKSASKVWAKEFGEESKRLWELAGPAIFTAISQYSLGALT 62

Query: 70  QAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRS 129
           Q F+G LG+LELAA+S+ N+VI G  FG++LGM SALETLCGQAYGA +  M+GIYMQRS
Sbjct: 63  QTFSGRLGELELAAVSVENSVISGLAFGVMLGMGSALETLCGQAYGAGQIRMMGIYMQRS 122

Query: 130 WIVLFICCIFLLPVYLFATPALKLLGQPSELAELAGKVAVLLVPLHFSFAFQFPLQRFLQ 189
           W++LF   +FLLPVY++A P L   G+   +++ AGK A+ ++P  F++A  FP+Q+FLQ
Sbjct: 123 WVILFTTALFLLPVYIWAPPILSFFGEAPHISKAAGKFALWMIPQLFAYAANFPIQKFLQ 182

Query: 190 SQLKTAVIAYVSFVALLVHVLVSWLLVYGLKLGLFGTAITINFSWWVLVFGLFIYTVSGG 249
           SQ K  V+A++S V L++H + SWL +   K GL G AIT+N SWW++V G  +Y +   
Sbjct: 183 SQRKVLVMAWISGVVLVIHAVFSWLFILYFKWGLVGAAITLNTSWWLIVIGQLLYILITK 242

Query: 250 CPLTWSGFSVEAFSGLWDFVKLSAASGVMLCLENWYYRILIVMTGNLENAKLAVDALSVC 309
               W+GFS+ AF  L+ FVKLS AS +MLCLE WY  +L+V+TG L N  + VDA+S+C
Sbjct: 243 SDGAWTGFSMLAFRDLYGFVKLSLASALMLCLEFWYLMVLVVVTGLLPNPLIPVDAISIC 302

Query: 310 MTINGWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATKVAVGTSVIIGLFFWIIIMIFD 369
           M I GW  MI + F     VRV+NELGAGN   AKF+  V   TS +IG+   I+++   
Sbjct: 303 MNIEGWTAMISIGFNAAISVRVSNELGAGNAALAKFSVIVVSITSTLIGIVCMIVVLATK 362

Query: 370 TQIALIFTSSEIVLKEVKKLSVLLAFTILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYL 429
                +FTSSE V  E  +++VLL FT+LLNS+QP+LSGVAVG+GWQ+ VAYVN+ CYY+
Sbjct: 363 DSFPYLFTSSEAVAAETTRIAVLLGFTVLLNSLQPVLSGVAVGAGWQALVAYVNIACYYI 422

Query: 430 IGLPLGFLMGWGFNQGVMGIWAGMIFGGTAIQTLILCIMTIRCDWDKEAEKASLHIRKWA 489
           IGLP G ++G+  + GV GIW GM+  G  +QTLIL  +    +W+KEAE+A   +++W 
Sbjct: 423 IGLPAGLVLGFTLDLGVQGIWGGMV-AGICLQTLILIGIIYFTNWNKEAEQAESRVQRWG 482