Tan0010118 (gene) Snake gourd v1

BLAST of Tan0010118 vs. ExPASy Swiss-Prot
Match: Q6DYE5 (UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2)

HSP 1 Score: 188.0 bits (476), Expect = 2.0e-46
Identity = 132/373 (35.39%), Postives = 215/373 (57.64%), Query Frame = 0

Query: 25  KNNIAITNGLSDKLNVNEEYKQAFRTNSYIE----------FTATNKYNNTLSSSSSSC- 84
           K++      L+ KL+VNEEYK+AFRTNSY+E           T+ +K +++  S SSS  
Sbjct: 21  KSDKECCRSLTSKLSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSD 80

Query: 85  ----------LLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLLQAVHQ 144
                     LL+P QE L+ + +     D ++ +L+V +F  S EA   C+ LLQ + Q
Sbjct: 81  LSFHSHFTDYLLDPPQETLDALMQ-----DSSLDNLIVTFFDLSSEACDVCETLLQCLQQ 140

Query: 145 TRINHAVVNGVIKLATRALMVDEDNQGYNDN--GLVCRELLASFSQLKNPFSFLTHN-QF 204
            +INH  +  V+K+  R     +  +   +    L+ +E L+ F+ LKNP   + +  QF
Sbjct: 141 IKINHNKIKRVMKIGKRVCNGAKTLECSPEMLCALIFQE-LSRFAALKNPLCRIVNEAQF 200

Query: 205 LGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALHSLVS 264
             +HD +S+LL KL+ ++   +RK+R  +  KK     L+I+++ +++ LL++ALHS++ 
Sbjct: 201 RIVHDANSDLLTKLTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILG 260

Query: 265 IVVAPGLIVCFVGSLKKKKRDDDKLAYSP-----ERVLRQMEIAARATYITMNDLDTLSR 324
           +  AP L+      L +KK+   ++  S      E++  Q++IAA+  +I +NDLDTLSR
Sbjct: 261 VFAAPALLGLCSFCLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSR 320

Query: 325 MAVRLDGEVEHLRAVAEMWMRS---SGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTI 366
           +A RL  E+EH + VA M  +S      +E L+EF   ++   +Q++EL+ H+YLCF TI
Sbjct: 321 LAGRLCDEIEHRKTVAAMCAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTI 380

BLAST of Tan0010118 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-26
Identity = 106/362 (29.28%), Postives = 169/362 (46.69%), Query Frame = 0

Query: 38  LNVNEEYKQAFRTNSYIEF----TATNKYNNTLSSSSSS--------------------C 97
           ++V EEY  AFRT SY  F       ++  + +SSSSSS                     
Sbjct: 69  VDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAHN 128

Query: 98  LLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLLQAVHQTRINHAVVNG 157
           LL+PD   +  + +    G H    LL DYF  +  AF  C  LL+ +H  R  +  +  
Sbjct: 129 LLDPDLNTITRILDVSRVGRH-TRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESLKP 188

Query: 158 VIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKNPFSFLTHNQFLGLHDTHSELLQK 217
                             N N L   +     S+  +PF   + ++   +      LL++
Sbjct: 189 KF-------------HSENHNSLALIDQFTEISKWFDPF-ISSGSRIQLIRSGCLYLLKR 248

Query: 218 LSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALHSLVSIVVAPGLIVCFVG 277
           L   R +T+ KL+L             I+  T   GLLVLAL + + + +A      F+ 
Sbjct: 249 LESRRDKTRAKLKL-------------INGLTHSSGLLVLALTTTLIVTIASHAFALFLA 308

Query: 278 --SLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTLSRMAVRLDGEVEHLRAVA 337
             +L   +     L     +   ++++AA+ TYI   DLDT+SR+  R++ EV H+RA+A
Sbjct: 309 APTLLASQFKPAGLRNKLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMA 368

Query: 338 EMWM-RSSGR----REILKEFVAEDDAIVEQMKELQPHIYLCFLTINRSRRLVMNEIVGA 369
           E W+ R SGR     E+ +E    +++  E++ EL+ HIYLCF+TINR+R L++ EI+ +
Sbjct: 369 EFWVGRGSGRVRGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDS 402

BLAST of Tan0010118 vs. ExPASy Swiss-Prot
Match: A2YH25 (Putative UPF0496 protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_023618 PE=3 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.4e-23
Identity = 110/380 (28.95%), Postives = 171/380 (45.00%), Query Frame = 0

Query: 38  LNVNEEYKQAFRTNSYIEFTA------TNKYNNTLSSSSSSC------------------ 97
           L V+EEY QAFR+ S+++  +      T+ +++   S+S+ C                  
Sbjct: 18  LAVDEEYNQAFRSKSFLDLWSHAHHHLTHTFSSFKLSTSTPCAGRGGAREDDFLHAGGDG 77

Query: 98  ------------------LLEPDQELLN-----DMFEGCNGGDHNVHHLLVDYFKASFEA 157
                             +LEP  E L                H V  LL++YF  + EA
Sbjct: 78  GAADDSEQSCSYTVLDDFVLEPSPESLARGARLQQRRRRRPRRHRVETLLIEYFDVTEEA 137

Query: 158 FKTCQLLLQAVHQTRINHAVVNGVIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKN 217
            + C  LL A+   R +H        L  R L++  D    +D     ++ LA   +L N
Sbjct: 138 CEACSALLAAIGAARRHH--------LTLRRLLLRLDGGDDDD----AKDALARHVRLDN 197

Query: 218 PFSFLTHNQFLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGL 277
           P S  + ++F  +H   S L  +L+  +   +R  R  RI +    + L+ + A  +V  
Sbjct: 198 PLSPGSLSEFHDVHARCSPLASRLAAAQRRLRRLARALRIARGTAAAALVGACAAAIVAA 257

Query: 278 LVLALHSLVSIVVAPGLIVCFVGSLKK--KKRDDDKLAYSP-ERVLRQMEIAARATYITM 337
           +VLA H+LV I VA            +   +R  +K++     R    ++ AAR  YI  
Sbjct: 258 VVLAAHALVGIGVAAAAFGATPAGAARWWARRAAEKVSSRHYARAGATLDAAARGAYIVG 317

Query: 338 NDLDTLSRMAVRLDGEVEHLRAVAEMWMRSSGRREILKEFVAEDDAIVE----QMKELQP 364
            DLDT+SRM  R   E+EH R VA + MR  G R +L+E   E++   E    Q+ EL+ 
Sbjct: 318 RDLDTVSRMVRRAHDELEHGRDVARIAMRGHGERPLLQEVAREEEECEEDLRAQLAELEE 377

BLAST of Tan0010118 vs. ExPASy Swiss-Prot
Match: Q5Z8N6 (Putative UPF0496 protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0718300 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.8e-23
Identity = 110/380 (28.95%), Postives = 171/380 (45.00%), Query Frame = 0

Query: 38  LNVNEEYKQAFRTNSYIEFTA------TNKYNNTLSSSSSSC------------------ 97
           L V+EEY QAFR+ S+++  +      T+ +++   S+S+ C                  
Sbjct: 18  LAVDEEYNQAFRSKSFLDLWSHAHHHLTHTFSSFKLSTSTPCAGRGGAREDDFLHAGGDG 77

Query: 98  ------------------LLEPDQELLN-----DMFEGCNGGDHNVHHLLVDYFKASFEA 157
                             +LEP  E L                H V  LL++YF  + EA
Sbjct: 78  GAADDSEQSCSYTVLDDFVLEPSPESLARGARLQQRRRRRPRRHRVETLLIEYFDVTEEA 137

Query: 158 FKTCQLLLQAVHQTRINHAVVNGVIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKN 217
            + C  LL A+   R +H        L  R L++  D    +D     ++ LA   +L N
Sbjct: 138 CEACSALLAAIGAARRHH--------LTLRRLLLRLDGGDDDD----AKDALARHVRLDN 197

Query: 218 PFSFLTHNQFLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGL 277
           P S  + ++F  +H   S L  +L+  +   +R  R  RI +    + L+ + A  +V  
Sbjct: 198 PLSPGSLSEFHDVHARCSPLASRLAAAQRRLRRLARALRIARGTAAAALVGACAAAIVAA 257

Query: 278 LVLALHSLVSIVVAPGLIVCFVGSLKK--KKRDDDKLAYSP-ERVLRQMEIAARATYITM 337
           +VLA H+LV I VA            +   +R  +K++     R    ++ AAR  YI  
Sbjct: 258 VVLAAHALVGIGVAAAAFGATPAGAARWWGRRAAEKVSSRHYARAGATLDAAARGAYIVG 317

Query: 338 NDLDTLSRMAVRLDGEVEHLRAVAEMWMRSSGRREILKEFVAEDDAIVE----QMKELQP 364
            DLDT+SRM  R   E+EH R VA + MR  G R +L+E   E++   E    Q+ EL+ 
Sbjct: 318 RDLDTVSRMVRRAHDELEHGRDVARIAMRGHGERPLLQEVAREEEECEEDLRAQLAELEE 377

BLAST of Tan0010118 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 2.0e-14
Identity = 94/370 (25.41%), Postives = 157/370 (42.43%), Query Frame = 0

Query: 35  SDKLNVNEEYKQAFRTNSYIEFTA-----TNKYNNTL---SSSSSSC------------- 94
           S   +  EEY  AFRT SY +F A     T  +   L         C             
Sbjct: 29  SSSFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFA 88

Query: 95  --LLEPDQELLNDMFEGCNGG--DHNVHHLLVDYFKASFEAFKTCQLLLQAVHQTRINHA 154
             LLEPDQ  +        G     +V  LL  Y+  +  A   C  LL+ +   R+ + 
Sbjct: 89  EHLLEPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYR 148

Query: 155 VVNGVIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKNPFSFLTHNQ--FLGLHDTH 214
                +K   R L  D    G  D            + L  PF+ L  +Q     +    
Sbjct: 149 ----PLKHTLRKLASDVGVSGLADVS----------AALGQPFTALAASQGRLREVQAGS 208

Query: 215 SELLQKLSHERSETQRKLR-LKRIRKKATTSCLMISNATVLVGLLVLALHSLVSIVVAPG 274
            +LL+ L   R + + ++R + R+R+  + S +       +VG  +  +H L +    P 
Sbjct: 209 GDLLRGLDAGRKKARHRIRSVARLRRALSVSFVTAVAVVAVVGACI-GVHILAAFAAFPM 268

Query: 275 LIVCFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTLSRMAVRLDGEVEH 334
           +   ++G      R       +  R L Q+E AA+ TYI   D++T+SR+  R+  E EH
Sbjct: 269 MSPAWLGERFFSGR-------AARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEH 328

Query: 335 LRAVAEMWMR--------SSGR--REILKEFVAEDDAIVEQMKELQPHIYLCFLTINRSR 367
           + A+  + +           GR  +E+L++    +++  +Q+ EL+ H++LCF+TIN++R
Sbjct: 329 MVALLRLCVEHRPAAGAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTINKAR 376

BLAST of Tan0010118 vs. NCBI nr
Match: XP_038884832.1 (UPF0496 protein At1g20180-like [Benincasa hispida])

HSP 1 Score: 449.9 bits (1156), Expect = 2.1e-122
Identity = 263/381 (69.03%), Postives = 301/381 (79.00%), Query Frame = 0

Query: 1   MKTSLWTSLRSSIFIFNQKDRSSS-KNNIAITNGLSDKLNVNEEYKQAFRTNSYIEFTAT 60
           MKTSLWTSLRS+  I N K+RSSS K N AI N LS+KLN+NEEY+ AFRTNSY+EFT T
Sbjct: 1   MKTSLWTSLRST--ISNLKERSSSLKKNKAIRNALSNKLNLNEEYQAAFRTNSYVEFTTT 60

Query: 61  -NKYNNTLSSSSSSCLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLL 120
            N  +NT SSS  SCLLEPDQ++     + CN G H VHHLL++YF ASFEAFK+CQLLL
Sbjct: 61  NNNKDNTSSSSLLSCLLEPDQDV---FLQSCNNGGH-VHHLLIEYFHASFEAFKSCQLLL 120

Query: 121 QAVHQTRINHA-VVNGVIKLATRALMVDEDNQGYNDNGLVCRE----LLASFSQLKNP-F 180
           QA+HQTRINHA +VN V KL T ALM D+D    ++ GLV RE    LL+SF QLKNP F
Sbjct: 121 QALHQTRINHAIIVNRVAKLTTTALMADDD----DNCGLVYREDQLMLLSSFCQLKNPNF 180

Query: 181 SFLTH--NQFLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGL 240
           S L H   QFL LH++HSELLQKL+ +R+E +R+LRLKRI K+A   C +ISNA VLV L
Sbjct: 181 SILAHIDGQFLRLHESHSELLQKLTCKRNEIRRELRLKRIWKRAARGCFLISNAAVLVAL 240

Query: 241 LVLALHSLVSIVVAPGLIVCFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDL 300
           L+LA HSL+ IV APGLI CFVG LKKKKR D K+ +S E VLRQMEIAARATYITMNDL
Sbjct: 241 LLLAFHSLIGIVAAPGLITCFVGLLKKKKRRDYKVVFSSEMVLRQMEIAARATYITMNDL 300

Query: 301 DTLSRMAVRLDGEVEHLRAVAEMWMRSSGRREILKEFVAEDDAIVEQMKELQPHIYLCFL 360
           DTLSRMA RLD EVEHLRAV EM++RSS R EILKEFV EDDA+VEQMK+LQ HIYLC L
Sbjct: 301 DTLSRMAARLDVEVEHLRAVCEMYIRSSRRCEILKEFVVEDDAVVEQMKDLQQHIYLCLL 360

Query: 361 TINRSRRLVMNEIVGANDQNG 372
           TINRSRRLVMNEI+G  DQ+G
Sbjct: 361 TINRSRRLVMNEIMGDRDQSG 371

BLAST of Tan0010118 vs. NCBI nr
Match: KAG6585525.1 (UPF0496 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 425.2 bits (1092), Expect = 5.6e-115
Identity = 248/375 (66.13%), Postives = 278/375 (74.13%), Query Frame = 0

Query: 1   MKTSLWTSLRSSIFIFNQKDRSSSKNNIAITNGLSDKLNVNEEYKQAFRTNSYIEFTATN 60
           MKTS W SL      FN+ DRSSS  N AI N LS KLN+NEEYKQAFRT S+IEFT TN
Sbjct: 1   MKTSHWISL------FNKTDRSSSDKNKAIMNALSRKLNINEEYKQAFRTTSFIEFTTTN 60

Query: 61  KYNNTLSSSSSS-------CLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKT 120
           K NNT+SSSSSS       C LEPDQ+LL +MF  CNG   +VH LL++YF+ASF+AFK 
Sbjct: 61  KDNNTISSSSSSLPPSPPPCRLEPDQKLLTEMFRSCNG--RHVHPLLLEYFEASFQAFKA 120

Query: 121 CQLLLQAVHQTRINHAVVNGVIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKNPFS 180
           CQ LLQ VHQTRINHA V    +LAT  +  D+DNQ  ++NG+V REL            
Sbjct: 121 CQFLLQTVHQTRINHAAV----ELAT-TMADDDDNQSSDNNGMVYREL------------ 180

Query: 181 FLTHNQFLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVL 240
                 FLGLHD HSELL+KLS  R++T+RKLRL+R  KK  T C +ISN  VLV LLVL
Sbjct: 181 ------FLGLHDIHSELLRKLSCLRNDTRRKLRLRRFGKKVATGCFVISNMAVLVALLVL 240

Query: 241 ALHSLVSIVVAPGLIVCFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTL 300
           ALHSLV IV  PGLIVCFVGSLKKK+RD        + +LRQMEIAARATYITMNDLDTL
Sbjct: 241 ALHSLVGIVAMPGLIVCFVGSLKKKRRDH-------KGILRQMEIAARATYITMNDLDTL 300

Query: 301 SRMAVRLDGEVEHLRAVAEMWMRSSGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTIN 360
           S+MAVRLDGEVEHLRAVA MWMRS G REILKEF+ EDDAIVEQ+KELQ HIYLC +TIN
Sbjct: 301 SQMAVRLDGEVEHLRAVAGMWMRSGGMREILKEFIEEDDAIVEQLKELQQHIYLCCVTIN 337

Query: 361 RSRRLVMNEIVGAND 369
           RSRRLVMNEIVGA D
Sbjct: 361 RSRRLVMNEIVGAKD 337

BLAST of Tan0010118 vs. NCBI nr
Match: XP_023002422.1 (UPF0496 protein At1g20180 isoform X1 [Cucurbita maxima])

HSP 1 Score: 424.9 bits (1091), Expect = 7.3e-115
Identity = 248/369 (67.21%), Postives = 274/369 (74.25%), Query Frame = 0

Query: 1   MKTSLWTSLRSSIFIFNQKDRSSSKNNIAITNGLSDKLNVNEEYKQAFRTNSYIEFTATN 60
           MKT+ W SL      FN+ DRSSS  N AI N LS KLN+NEEYKQAFRT S+IEFT T 
Sbjct: 1   MKTAHWISL------FNKTDRSSSDKNKAIMNALSRKLNINEEYKQAFRTTSFIEFTTTY 60

Query: 61  KYNNTLSS-SSSSCLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLLQ 120
           K NNT SS     C LEPDQ+LL+DM   CNG   +VH LL++YF+ASF+AFK CQLLLQ
Sbjct: 61  KDNNTSSSPPPPPCRLEPDQKLLHDMLRSCNG--RHVHPLLLEYFEASFQAFKACQLLLQ 120

Query: 121 AVHQTRINHAVVNGVIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKNPFSFLTHNQ 180
           AVHQTRINHAVV    KLAT A M D+DNQ  N+NG+V +EL                  
Sbjct: 121 AVHQTRINHAVV----KLATTASMADDDNQSSNNNGMVYKEL------------------ 180

Query: 181 FLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALHSLV 240
           FL LHD HSELL+KLS  R+ET++KLRL+  RKK  T C +ISNA VL+ LLVLALHSLV
Sbjct: 181 FLSLHDLHSELLRKLSCLRNETRKKLRLRCFRKKVATGCFVISNAAVLITLLVLALHSLV 240

Query: 241 SIVVAPGLIVCFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTLSRMAVR 300
            IV  PGLIVCFVGSLKKK+RD        + +LRQMEIAARATYITMNDLDTLSRMAVR
Sbjct: 241 GIVAMPGLIVCFVGSLKKKRRDH-------KGILRQMEIAARATYITMNDLDTLSRMAVR 300

Query: 301 LDGEVEHLRAVAEMWMRSSGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTINRSRRLV 360
           LDGEVEHLRAV  MWMRS   REILKEFV EDDAIVEQMKELQ HIYLCF+TINRSRRLV
Sbjct: 301 LDGEVEHLRAVGGMWMRSGRMREILKEFVEEDDAIVEQMKELQQHIYLCFVTINRSRRLV 332

Query: 361 MNEIVGAND 369
           MNEIV A D
Sbjct: 361 MNEIVEAKD 332

BLAST of Tan0010118 vs. NCBI nr
Match: XP_022951748.1 (UPF0496 protein At1g20180 [Cucurbita moschata])

HSP 1 Score: 421.8 bits (1083), Expect = 6.2e-114
Identity = 249/375 (66.40%), Postives = 277/375 (73.87%), Query Frame = 0

Query: 1   MKTSLWTSLRSSIFIFNQKDRSSSKNNIAITNGLSDKLNVNEEYKQAFRTNSYIEFTATN 60
           MKTS W SL      FN+ DRSSS  N AI N LS KLN+NEEYKQAFRT S+IEFT TN
Sbjct: 1   MKTSHWISL------FNKTDRSSSDKNKAIMNALSRKLNINEEYKQAFRTTSFIEFTTTN 60

Query: 61  KYNNTLSSSSSS-------CLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKT 120
           K NNT+SSSSSS       C LEPDQ+LL +MF  CNG   +VH LL++YF+ASF+AFK 
Sbjct: 61  KDNNTISSSSSSLPPSPPPCRLEPDQKLLTEMFRSCNG--RHVHPLLLEYFEASFQAFKA 120

Query: 121 CQLLLQAVHQTRINHAVVNGVIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKNPFS 180
           CQ LLQ VHQTRINHA V    +LAT  +  D+DNQ  ++NG+V REL            
Sbjct: 121 CQFLLQTVHQTRINHAAV----ELAT-TMADDDDNQSSDNNGMVYREL------------ 180

Query: 181 FLTHNQFLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVL 240
                 FLGLHD HSELL+KLS  R+ T+RKLRL+R  KK  T C +ISN  VLV LLVL
Sbjct: 181 ------FLGLHDIHSELLRKLSCLRN-TRRKLRLRRFGKKVATGCFVISNTAVLVALLVL 240

Query: 241 ALHSLVSIVVAPGLIVCFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTL 300
           ALHSLV IV  PGLIVCFVGSLKKK+RD        + +LRQMEIAARATYITMNDLDTL
Sbjct: 241 ALHSLVGIVAMPGLIVCFVGSLKKKRRDH-------KGILRQMEIAARATYITMNDLDTL 300

Query: 301 SRMAVRLDGEVEHLRAVAEMWMRSSGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTIN 360
           SRMAVRLDGEVEHLRAVA MWMRS G REILKEF+ EDDAIVEQ+KELQ HIYLC +TIN
Sbjct: 301 SRMAVRLDGEVEHLRAVAGMWMRSGGMREILKEFIEEDDAIVEQLKELQQHIYLCCVTIN 336

Query: 361 RSRRLVMNEIVGAND 369
           RSRRLVMNEIVGA D
Sbjct: 361 RSRRLVMNEIVGAKD 336

BLAST of Tan0010118 vs. NCBI nr
Match: XP_023538283.1 (UPF0496 protein At1g20180 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421.0 bits (1081), Expect = 1.1e-113
Identity = 247/372 (66.40%), Postives = 273/372 (73.39%), Query Frame = 0

Query: 1   MKTSLWTSLRSSIFIFNQKDRSSSKNNIAITNGLSDKLNVNEEYKQAFRTNSYIEFTATN 60
           MKTS W SL      FN+ DRSSS  N AI N LS KLN+NEEYKQAFRT S+IEFT TN
Sbjct: 1   MKTSHWISL------FNKTDRSSSDKNKAIMNALSRKLNINEEYKQAFRTTSFIEFTTTN 60

Query: 61  KYNNTLSS----SSSSCLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQL 120
           K NNT+SS    S   C LEPDQ+LL +MF+ CN G H  H LL++YF+ASF+AFK CQ 
Sbjct: 61  KDNNTISSSPPPSPPPCRLEPDQKLLTEMFQSCN-GRHVGHPLLLEYFEASFQAFKACQF 120

Query: 121 LLQAVHQTRINHAVVNGVIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKNPFSFLT 180
           LLQ VHQTRINHA V    +LAT   M D+DNQ  ++NG+V REL               
Sbjct: 121 LLQTVHQTRINHATV----ELAT--TMADDDNQSSDNNGMVYREL--------------- 180

Query: 181 HNQFLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALH 240
              FL LHDTHSELL+KLS  R+ T+RKLRL+R  KK    C +ISN  VLV LLVLALH
Sbjct: 181 ---FLSLHDTHSELLRKLSCLRNNTRRKLRLRRFGKKVARGCFVISNTAVLVALLVLALH 240

Query: 241 SLVSIVVAPGLIVCFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTLSRM 300
           SLV IV  PGLIVCFVGSLKKK+RD        + +LRQMEIAARATYITMNDLDTLSRM
Sbjct: 241 SLVGIVAMPGLIVCFVGSLKKKRRDH-------KGILRQMEIAARATYITMNDLDTLSRM 300

Query: 301 AVRLDGEVEHLRAVAEMWMRSSGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTINRSR 360
           AVRLDGEVEHLRAVA MWMRS G REILKEFV EDDAIVEQ+KELQ HIYLC +TINRSR
Sbjct: 301 AVRLDGEVEHLRAVAGMWMRSGGMREILKEFVEEDDAIVEQLKELQQHIYLCCVTINRSR 334

Query: 361 RLVMNEIVGAND 369
           RLVMNEIVGA D
Sbjct: 361 RLVMNEIVGAKD 334

BLAST of Tan0010118 vs. ExPASy TrEMBL
Match: A0A6J1KJG4 (UPF0496 protein At1g20180 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496268 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 3.5e-115
Identity = 248/369 (67.21%), Postives = 274/369 (74.25%), Query Frame = 0

Query: 1   MKTSLWTSLRSSIFIFNQKDRSSSKNNIAITNGLSDKLNVNEEYKQAFRTNSYIEFTATN 60
           MKT+ W SL      FN+ DRSSS  N AI N LS KLN+NEEYKQAFRT S+IEFT T 
Sbjct: 1   MKTAHWISL------FNKTDRSSSDKNKAIMNALSRKLNINEEYKQAFRTTSFIEFTTTY 60

Query: 61  KYNNTLSS-SSSSCLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLLQ 120
           K NNT SS     C LEPDQ+LL+DM   CNG   +VH LL++YF+ASF+AFK CQLLLQ
Sbjct: 61  KDNNTSSSPPPPPCRLEPDQKLLHDMLRSCNG--RHVHPLLLEYFEASFQAFKACQLLLQ 120

Query: 121 AVHQTRINHAVVNGVIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKNPFSFLTHNQ 180
           AVHQTRINHAVV    KLAT A M D+DNQ  N+NG+V +EL                  
Sbjct: 121 AVHQTRINHAVV----KLATTASMADDDNQSSNNNGMVYKEL------------------ 180

Query: 181 FLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALHSLV 240
           FL LHD HSELL+KLS  R+ET++KLRL+  RKK  T C +ISNA VL+ LLVLALHSLV
Sbjct: 181 FLSLHDLHSELLRKLSCLRNETRKKLRLRCFRKKVATGCFVISNAAVLITLLVLALHSLV 240

Query: 241 SIVVAPGLIVCFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTLSRMAVR 300
            IV  PGLIVCFVGSLKKK+RD        + +LRQMEIAARATYITMNDLDTLSRMAVR
Sbjct: 241 GIVAMPGLIVCFVGSLKKKRRDH-------KGILRQMEIAARATYITMNDLDTLSRMAVR 300

Query: 301 LDGEVEHLRAVAEMWMRSSGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTINRSRRLV 360
           LDGEVEHLRAV  MWMRS   REILKEFV EDDAIVEQMKELQ HIYLCF+TINRSRRLV
Sbjct: 301 LDGEVEHLRAVGGMWMRSGRMREILKEFVEEDDAIVEQMKELQQHIYLCFVTINRSRRLV 332

Query: 361 MNEIVGAND 369
           MNEIV A D
Sbjct: 361 MNEIVEAKD 332

BLAST of Tan0010118 vs. ExPASy TrEMBL
Match: A0A6J1GIK9 (UPF0496 protein At1g20180 OS=Cucurbita moschata OX=3662 GN=LOC111454488 PE=3 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 3.0e-114
Identity = 249/375 (66.40%), Postives = 277/375 (73.87%), Query Frame = 0

Query: 1   MKTSLWTSLRSSIFIFNQKDRSSSKNNIAITNGLSDKLNVNEEYKQAFRTNSYIEFTATN 60
           MKTS W SL      FN+ DRSSS  N AI N LS KLN+NEEYKQAFRT S+IEFT TN
Sbjct: 1   MKTSHWISL------FNKTDRSSSDKNKAIMNALSRKLNINEEYKQAFRTTSFIEFTTTN 60

Query: 61  KYNNTLSSSSSS-------CLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKT 120
           K NNT+SSSSSS       C LEPDQ+LL +MF  CNG   +VH LL++YF+ASF+AFK 
Sbjct: 61  KDNNTISSSSSSLPPSPPPCRLEPDQKLLTEMFRSCNG--RHVHPLLLEYFEASFQAFKA 120

Query: 121 CQLLLQAVHQTRINHAVVNGVIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKNPFS 180
           CQ LLQ VHQTRINHA V    +LAT  +  D+DNQ  ++NG+V REL            
Sbjct: 121 CQFLLQTVHQTRINHAAV----ELAT-TMADDDDNQSSDNNGMVYREL------------ 180

Query: 181 FLTHNQFLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVL 240
                 FLGLHD HSELL+KLS  R+ T+RKLRL+R  KK  T C +ISN  VLV LLVL
Sbjct: 181 ------FLGLHDIHSELLRKLSCLRN-TRRKLRLRRFGKKVATGCFVISNTAVLVALLVL 240

Query: 241 ALHSLVSIVVAPGLIVCFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTL 300
           ALHSLV IV  PGLIVCFVGSLKKK+RD        + +LRQMEIAARATYITMNDLDTL
Sbjct: 241 ALHSLVGIVAMPGLIVCFVGSLKKKRRDH-------KGILRQMEIAARATYITMNDLDTL 300

Query: 301 SRMAVRLDGEVEHLRAVAEMWMRSSGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTIN 360
           SRMAVRLDGEVEHLRAVA MWMRS G REILKEF+ EDDAIVEQ+KELQ HIYLC +TIN
Sbjct: 301 SRMAVRLDGEVEHLRAVAGMWMRSGGMREILKEFIEEDDAIVEQLKELQQHIYLCCVTIN 336

Query: 361 RSRRLVMNEIVGAND 369
           RSRRLVMNEIVGA D
Sbjct: 361 RSRRLVMNEIVGAKD 336

BLAST of Tan0010118 vs. ExPASy TrEMBL
Match: A0A1S3BDA5 (UPF0496 protein At1g20180-like OS=Cucumis melo OX=3656 GN=LOC103488392 PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 3.7e-112
Identity = 251/386 (65.03%), Postives = 300/386 (77.72%), Query Frame = 0

Query: 1   MKT-SLWTSLRSSIFIFNQKDRSSSKNNIAITNGLSDKLNVNEEYKQAFRTNSYIEFTAT 60
           MKT S+WTSLRS+  I N K+RSSSK N A  N LS+KLN+NEEY+ AFRTNSY+EFTAT
Sbjct: 1   MKTSSVWTSLRSA--ISNLKERSSSKKNKANANALSNKLNLNEEYQAAFRTNSYVEFTAT 60

Query: 61  NKYNNTLSSSSS---SCLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQL 120
              NNT SSSSS   SCLL+PDQ++L+++    NGG  + H LL+ YF+ASFEAF+TC+L
Sbjct: 61  T--NNTSSSSSSSLMSCLLQPDQDMLHNITS--NGGHVHNHDLLIQYFQASFEAFETCRL 120

Query: 121 LLQAVHQTRINHAV-VNGVIKLATRALMVDEDNQGYNDNGLVCRE-----LLASFSQLKN 180
           LLQA++QT+INH + VN  IKL   AL  ++D+ G  ++GLV  E      L+SFS LK+
Sbjct: 121 LLQALNQTKINHHILVNATIKLTAMALRAEDDDNG--NHGLVYGEDDRLISLSSFSHLKD 180

Query: 181 P-FSFLTH--NQFLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVL 240
           P FS LT    +FL LH++HSELLQKL+ +R+ET+ KLRLKR+RK+A   CL+ISNA VL
Sbjct: 181 PNFSILTRIGGRFLALHESHSELLQKLASKRNETRSKLRLKRLRKRAAKGCLLISNAAVL 240

Query: 241 VGLLVLALHSLVSIVVAPGLIV-CFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYIT 300
           V LL+LALHSLV IV APGL++ CFVG LKKK + D KL ++ E  L+QMEIAAR TYIT
Sbjct: 241 VALLLLALHSLVGIVAAPGLLIACFVGLLKKKVKRDYKLPFTLETSLQQMEIAARGTYIT 300

Query: 301 MNDLDTLSRMAVRLDGEVEHLRAVAEMWMRSS-GRREILKEFVAEDDAIVEQMKELQPHI 360
           MNDLDTLSRMA RLD EVEHLRAV EMWMRSS    EILKEFV ED+AIVEQMKELQ HI
Sbjct: 301 MNDLDTLSRMAARLDVEVEHLRAVGEMWMRSSRSGCEILKEFVVEDEAIVEQMKELQQHI 360

Query: 361 YLCFLTINRSRRLVMNEIVGANDQNG 372
           YLCF TINRSRRLVMNEI+G  DQ+G
Sbjct: 361 YLCFHTINRSRRLVMNEIMGDMDQSG 378

BLAST of Tan0010118 vs. ExPASy TrEMBL
Match: A0A0A0LN80 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G346030 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.5e-110
Identity = 246/383 (64.23%), Postives = 293/383 (76.50%), Query Frame = 0

Query: 1   MKT-SLWTSLRSSIFIFNQKDRSSSKNNIAITNGLSDKLNVNEEYKQAFRTNSYIEFTAT 60
           MKT S+WTSLRS+I I   ++RSSSK N A  N LS+KLN+NEEY+ AFRTNSY+EFTA 
Sbjct: 1   MKTSSVWTSLRSAISILKGRERSSSKKNKANANALSNKLNLNEEYQAAFRTNSYVEFTAI 60

Query: 61  NKYNNTLSSSS-SSCLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLL 120
              NNT S SS  SCLLEPDQ++L       NGG  + H LL+ YF+ASFEAF+TCQLLL
Sbjct: 61  T--NNTSSPSSLISCLLEPDQDMLLHNITS-NGGHVHDHDLLIQYFQASFEAFETCQLLL 120

Query: 121 QAVHQTRINHAV-VNGVIKLATRALMVDEDNQGYNDNGLVCRE-----LLASFSQLKNP- 180
           QA++QT+INH + VN  +KL T AL   +D+ G  ++G V  E     L + FS+LKNP 
Sbjct: 121 QALNQTKINHHILVNATVKLTTMALRAGDDDNG--NHGRVYGEDDPLILSSFFSRLKNPN 180

Query: 181 FSFLTH--NQFLGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVG 240
           FS L    ++FL LH++HSELLQKL+ +++ET+RKLRLKRIRK+    CL+ISNA VLV 
Sbjct: 181 FSILNQIGSRFLALHESHSELLQKLASKQNETRRKLRLKRIRKRVAKGCLLISNAAVLVA 240

Query: 241 LLVLALHSLVSIVVAPGLIV-CFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMN 300
           LL+LALHSLV IV APGL++ CFVG LKKK + D KL ++PE  L+QMEIAAR TYITMN
Sbjct: 241 LLLLALHSLVGIVAAPGLLIACFVGLLKKKVKRDYKLPFTPETSLQQMEIAARGTYITMN 300

Query: 301 DLDTLSRMAVRLDGEVEHLRAVAEMWMRSS-GRREILKEFVAEDDAIVEQMKELQPHIYL 360
           DLDTLSRMA RLD EVEHLRAV EMWMRSS  R EILKEFV ED+AIVEQMKELQ HIYL
Sbjct: 301 DLDTLSRMAARLDVEVEHLRAVGEMWMRSSRSRCEILKEFVVEDEAIVEQMKELQQHIYL 360

Query: 361 CFLTINRSRRLVMNEIVGANDQN 371
           CF TINRSRRLVM+E +G  DQ+
Sbjct: 361 CFHTINRSRRLVMDETMGDMDQS 378

BLAST of Tan0010118 vs. ExPASy TrEMBL
Match: A0A6J1KTI4 (UPF0496 protein At1g20180 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496268 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 5.0e-109
Identity = 232/338 (68.64%), Postives = 256/338 (75.74%), Query Frame = 0

Query: 32  NGLSDKLNVNEEYKQAFRTNSYIEFTATNKYNNTLSS-SSSSCLLEPDQELLNDMFEGCN 91
           N LS KLN+NEEYKQAFRT S+IEFT T K NNT SS     C LEPDQ+LL+DM   CN
Sbjct: 2   NALSRKLNINEEYKQAFRTTSFIEFTTTYKDNNTSSSPPPPPCRLEPDQKLLHDMLRSCN 61

Query: 92  GGDHNVHHLLVDYFKASFEAFKTCQLLLQAVHQTRINHAVVNGVIKLATRALMVDEDNQG 151
           G   +VH LL++YF+ASF+AFK CQLLLQAVHQTRINHAVV    KLAT A M D+DNQ 
Sbjct: 62  G--RHVHPLLLEYFEASFQAFKACQLLLQAVHQTRINHAVV----KLATTASMADDDNQS 121

Query: 152 YNDNGLVCRELLASFSQLKNPFSFLTHNQFLGLHDTHSELLQKLSHERSETQRKLRLKRI 211
            N+NG+V +EL                  FL LHD HSELL+KLS  R+ET++KLRL+  
Sbjct: 122 SNNNGMVYKEL------------------FLSLHDLHSELLRKLSCLRNETRKKLRLRCF 181

Query: 212 RKKATTSCLMISNATVLVGLLVLALHSLVSIVVAPGLIVCFVGSLKKKKRDDDKLAYSPE 271
           RKK  T C +ISNA VL+ LLVLALHSLV IV  PGLIVCFVGSLKKK+RD        +
Sbjct: 182 RKKVATGCFVISNAAVLITLLVLALHSLVGIVAMPGLIVCFVGSLKKKRRDH-------K 241

Query: 272 RVLRQMEIAARATYITMNDLDTLSRMAVRLDGEVEHLRAVAEMWMRSSGRREILKEFVAE 331
            +LRQMEIAARATYITMNDLDTLSRMAVRLDGEVEHLRAV  MWMRS   REILKEFV E
Sbjct: 242 GILRQMEIAARATYITMNDLDTLSRMAVRLDGEVEHLRAVGGMWMRSGRMREILKEFVEE 301

Query: 332 DDAIVEQMKELQPHIYLCFLTINRSRRLVMNEIVGAND 369
           DDAIVEQMKELQ HIYLCF+TINRSRRLVMNEIV A D
Sbjct: 302 DDAIVEQMKELQQHIYLCFVTINRSRRLVMNEIVEAKD 308

BLAST of Tan0010118 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 188.0 bits (476), Expect = 1.4e-47
Identity = 132/373 (35.39%), Postives = 215/373 (57.64%), Query Frame = 0

Query: 25  KNNIAITNGLSDKLNVNEEYKQAFRTNSYIE----------FTATNKYNNTLSSSSSSC- 84
           K++      L+ KL+VNEEYK+AFRTNSY+E           T+ +K +++  S SSS  
Sbjct: 21  KSDKECCRSLTSKLSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSD 80

Query: 85  ----------LLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLLQAVHQ 144
                     LL+P QE L+ + +     D ++ +L+V +F  S EA   C+ LLQ + Q
Sbjct: 81  LSFHSHFTDYLLDPPQETLDALMQ-----DSSLDNLIVTFFDLSSEACDVCETLLQCLQQ 140

Query: 145 TRINHAVVNGVIKLATRALMVDEDNQGYNDN--GLVCRELLASFSQLKNPFSFLTHN-QF 204
            +INH  +  V+K+  R     +  +   +    L+ +E L+ F+ LKNP   + +  QF
Sbjct: 141 IKINHNKIKRVMKIGKRVCNGAKTLECSPEMLCALIFQE-LSRFAALKNPLCRIVNEAQF 200

Query: 205 LGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALHSLVS 264
             +HD +S+LL KL+ ++   +RK+R  +  KK     L+I+++ +++ LL++ALHS++ 
Sbjct: 201 RIVHDANSDLLTKLTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILG 260

Query: 265 IVVAPGLIVCFVGSLKKKKRDDDKLAYSP-----ERVLRQMEIAARATYITMNDLDTLSR 324
           +  AP L+      L +KK+   ++  S      E++  Q++IAA+  +I +NDLDTLSR
Sbjct: 261 VFAAPALLGLCSFCLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSR 320

Query: 325 MAVRLDGEVEHLRAVAEMWMRS---SGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTI 366
           +A RL  E+EH + VA M  +S      +E L+EF   ++   +Q++EL+ H+YLCF TI
Sbjct: 321 LAGRLCDEIEHRKTVAAMCAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTI 380

BLAST of Tan0010118 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 158.3 bits (399), Expect = 1.2e-38
Identity = 124/373 (33.24%), Postives = 197/373 (52.82%), Query Frame = 0

Query: 25  KNNIAITNGLSDKLNVNEEYKQAFRTNSYIE----------FTATNKYNNTLSSSSSSC- 84
           K++      L+ KL+VNEEYK+AFRTNSY+E           T+ +K +++  S SSS  
Sbjct: 21  KSDKECCRSLTSKLSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSD 80

Query: 85  ----------LLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLLQAVHQ 144
                     LL+P QE L+ + +     D ++ +L+V +F  S EA   C+ LLQ + Q
Sbjct: 81  LSFHSHFTDYLLDPPQETLDALMQ-----DSSLDNLIVTFFDLSSEACDVCETLLQCLQQ 140

Query: 145 TRINHAVVNGVIKLATRALMVDEDNQGYNDN--GLVCRELLASFSQLKNPFSFLTHN-QF 204
            +INH  +  V+K+  R     +  +   +    L+ +E L+ F+ LKNP   + +  QF
Sbjct: 141 IKINHNKIKRVMKIGKRVCNGAKTLECSPEMLCALIFQE-LSRFAALKNPLCRIVNEAQF 200

Query: 205 LGLHDTHSELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALHSLVS 264
             +HD +S+LL KL+ ++         +RIR+K                       S++ 
Sbjct: 201 RIVHDANSDLLTKLTSKK---------RRIRRKI----------------------SILG 260

Query: 265 IVVAPGLIVCFVGSLKKKKRDDDKLAYSP-----ERVLRQMEIAARATYITMNDLDTLSR 324
           +  AP L+      L +KK+   ++  S      E++  Q++IAA+  +I +NDLDTLSR
Sbjct: 261 VFAAPALLGLCSFCLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSR 320

Query: 325 MAVRLDGEVEHLRAVAEMWMRS---SGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTI 366
           +A RL  E+EH + VA M  +S      +E L+EF   ++   +Q++EL+ H+YLCF TI
Sbjct: 321 LAGRLCDEIEHRKTVAAMCAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTI 356

BLAST of Tan0010118 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 122.5 bits (306), Expect = 7.2e-28
Identity = 106/362 (29.28%), Postives = 169/362 (46.69%), Query Frame = 0

Query: 38  LNVNEEYKQAFRTNSYIEF----TATNKYNNTLSSSSSS--------------------C 97
           ++V EEY  AFRT SY  F       ++  + +SSSSSS                     
Sbjct: 69  VDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAHN 128

Query: 98  LLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLLQAVHQTRINHAVVNG 157
           LL+PD   +  + +    G H    LL DYF  +  AF  C  LL+ +H  R  +  +  
Sbjct: 129 LLDPDLNTITRILDVSRVGRH-TRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESLKP 188

Query: 158 VIKLATRALMVDEDNQGYNDNGLVCRELLASFSQLKNPFSFLTHNQFLGLHDTHSELLQK 217
                             N N L   +     S+  +PF   + ++   +      LL++
Sbjct: 189 KF-------------HSENHNSLALIDQFTEISKWFDPF-ISSGSRIQLIRSGCLYLLKR 248

Query: 218 LSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALHSLVSIVVAPGLIVCFVG 277
           L   R +T+ KL+L             I+  T   GLLVLAL + + + +A      F+ 
Sbjct: 249 LESRRDKTRAKLKL-------------INGLTHSSGLLVLALTTTLIVTIASHAFALFLA 308

Query: 278 --SLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTLSRMAVRLDGEVEHLRAVA 337
             +L   +     L     +   ++++AA+ TYI   DLDT+SR+  R++ EV H+RA+A
Sbjct: 309 APTLLASQFKPAGLRNKLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMA 368

Query: 338 EMWM-RSSGR----REILKEFVAEDDAIVEQMKELQPHIYLCFLTINRSRRLVMNEIVGA 369
           E W+ R SGR     E+ +E    +++  E++ EL+ HIYLCF+TINR+R L++ EI+ +
Sbjct: 369 EFWVGRGSGRVRGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDS 402

BLAST of Tan0010118 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 65.9 bits (159), Expect = 8.1e-11
Identity = 84/361 (23.27%), Postives = 157/361 (43.49%), Query Frame = 0

Query: 22  SSSKNNIAITNGLSDKLNVNEEYKQAFRTNSY--------IEFTATNKYNNTLSSSSS-- 81
           S+S+N+ +I+   S   N++ E   AF+T SY        +    T  ++  +       
Sbjct: 32  STSRNDASIS---SPTFNLSRELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIELL 91

Query: 82  -SCLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLLQAVHQTRINHAV 141
            S +L+P++E + +           + +L+  YF+ S +A + C  L Q VH  R  H +
Sbjct: 92  LSQVLQPNKECVQEAIRHVK--QTTLTNLVSTYFQHSEDATRLCLNLYQNVHSAR--HHL 151

Query: 142 VNGVIKLATRALMVDEDNQGYNDNGL--VCRELLASFSQLKNPFSFLTHNQFLGLHDTHS 201
              ++ L     +   D+    D  L  +  ++       +NPFS      F       S
Sbjct: 152 YTPLLDLFN---IFPGDSLPAIDESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFS 211

Query: 202 ELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALHSLVSIVVAPGLI 261
           +L   L     +++ ++RL       ++ CL+ +   V    +V+A H+L  ++V  G +
Sbjct: 212 QLKHNLDRRLRKSRSRVRLIHHATAGSSLCLVAAVVAVAASAVVIASHALPILLVVAGPL 271

Query: 262 VC--FVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTLSRMAVRLDGEVEH 321
                  S K+K+  +          + Q+  A++ T++   DLDT+ R+  RL   +E+
Sbjct: 272 CSPYLPHSFKRKELTN----------ICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEY 331

Query: 322 ----LRAVAEMWMRSSGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTINRSRRLVMNE 364
               +R   E        +EILK        +  Q+K+L+ HI L F  +N++R L++ E
Sbjct: 332 DKFLIRLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTE 372

BLAST of Tan0010118 vs. TAIR 10
Match: AT3G19330.3 (Protein of unknown function (DUF677) )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 82/359 (22.84%), Postives = 145/359 (40.39%), Query Frame = 0

Query: 22  SSSKNNIAITNGLSDKLNVNEEYKQAFRTNSY--------IEFTATNKYNNTLSSSSS-- 81
           S+S+N+ +I+   S   N++ E   AF+T SY        +    T  ++  +       
Sbjct: 32  STSRNDASIS---SPTFNLSRELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIELL 91

Query: 82  -SCLLEPDQELLNDMFEGCNGGDHNVHHLLVDYFKASFEAFKTCQLLLQAVHQTRINHAV 141
            S +L+P++E + +           + +L+  YF+ S +A + C  L Q VH  R  H +
Sbjct: 92  LSQVLQPNKECVQEAIRHVK--QTTLTNLVSTYFQHSEDATRLCLNLYQNVHSAR--HHL 151

Query: 142 VNGVIKLATRALMVDEDNQGYNDNGL--VCRELLASFSQLKNPFSFLTHNQFLGLHDTHS 201
              ++ L     +   D+    D  L  +  ++       +NPFS      F       S
Sbjct: 152 YTPLLDLFN---IFPGDSLPAIDESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQLCFS 211

Query: 202 ELLQKLSHERSETQRKLRLKRIRKKATTSCLMISNATVLVGLLVLALHSLVSIVVAPGLI 261
           +L   L        R+LR  R R +                   L  H+    + +P L 
Sbjct: 212 QLKHNL-------DRRLRKSRSRVR-------------------LIHHATAGPLCSPYL- 271

Query: 262 VCFVGSLKKKKRDDDKLAYSPERVLRQMEIAARATYITMNDLDTLSRMAVRLDGEVEH-- 321
                S K+K+  +          + Q+  A++ T++   DLDT+ R+  RL   +E+  
Sbjct: 272 ---PHSFKRKELTN----------ICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDK 331

Query: 322 --LRAVAEMWMRSSGRREILKEFVAEDDAIVEQMKELQPHIYLCFLTINRSRRLVMNEI 364
             +R   E        +EILK        +  Q+K+L+ HI L F  +N++R L++ EI
Sbjct: 332 FLIRLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 340