Tan0009550 (gene) Snake gourd v1

Overview


Name	Tan0009550
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Procollagen-proline 4-dioxygenase
Location	LG07: 7290145 .. 7292833 (+)
RNA-Seq Expression	Tan0009550
Synteny	Tan0009550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCTCGTCTTAACTTTCTGCTTCTTTTAGTGACTGCATTTTCATTCTCAAGCTGCCTTGCACGAAGGTGATTACGAACGCCATTTTACATCTCACTTGTGTCTTATTCATTTCGAAGTCTTAGGTTTTACAGATCCGCATCGCCGCACCTTCTGTGTGGTTTACATTGGATTCTCATCAATTTCATTTACGGATGTACAATTGGAATTGTGTTCACTTCCTAATACTGAATTTGCTGAATGATTTGTTGATGCATGATTGAGTTTGATATTGCGTGTTCTTTTTCTTTCCCTTGAATCTGCTCTGTTCTTTGTGCCAGCAATTTGATTAGTGGTCGGAAGGGTTTAAGGGACCAGTTGGTCGACAGTGTTCCTTTGAGCTACTCAAATCATTCTGGAAGAATCGACCCTTCAAGAGTTGTCCAAGTCTCTTGGCGACCAAGGTCAGGTACTTGCAGAATCTCTCATAAGATATTGTTGACGTGAAACTGTGAACTTATTTAGAATTATCACATCAACTTCAAAGCATATTGATTAATTTAGTTGGAAAATCTGATTTAACTTTTCTTGCTGTAGGGTTTTCTTGTATAAAGGTTTTCTCTCAGATGCGGAATGTGATCACCTTATTTCTTTGGTATTTCTCTGCCCTGTTCTTATTAGTATTTTGAAAATGCTTCAGCTGTTTCTGACGCGCATATAATTTTTTTGGCTTAGGCTTCAAATTCAGAAGATAAACCTTCTGGGAACAGTGCTGGTTCTAGGAACACTGTCTCAACCAAATTCCTTAACAATTCAGGAGCCATTTTAAACACAACAGTACATTCCTGTGCCTTCTGAAGTTTACGCAATAGTTTGATAGTACATCTTAGTGGTCAAATTTCTATTACATAGCAATATAGCATGAGATTTGTTTATCTTGTTTTCTTAAAATTTATGTAAAAGTAGTATTTTTTTCAATCTTATATTGTTTTCTGTCGGTATCTACGTGTGAGCAATTATGCAGTCCATCAGTTTTAGAACTTTTTCTGTCGTTAATGTTTTATTTATTTCCGGTTATTTAAAATACAACAACAACTGTACATGGATGAACAGTTTCGTCTATCCATGGCAATTGCTTGAGTTTAATTAACCGTTCAATGTATTTTCTATTCAAATATGTTCAGTTGAAAGCTTTCTGTAGATATTTTTCAGTGGTACTACCAAATATCCGTGTTGCATGTTAGAATGGTACACCGACTATTGTTGCTTTTAATCTATTATTGAAGATGTGGGGTTGTATTTCCAAAGGTTCCTTCTCTCATTCCCTAAATTTCCTGATGGGTCACTTTTAGGATTGATGACATCTCTTATGCTGTCAACTCATTCTAATACTCATAGAAGCATGCTTTGCTGATTTGTTTTCTGGGGAACCGGTAGTCTCTTTAAAGGAATCTCAAACAATTAGCTCTAGATTTCCTATGAAGTCAAACATTTCTTCTTTGTGCTTTTGTAGATACAAGCACTAAAAATGTGGAGGATGGTAGTATAACGTAACCTATGATAATTTATGCAAGATTCCATAATTAGTTCTGAAATTCTTGTTCTTGATGACTCGAGTTGTTGGCATCAATTGTCTTTCAAAATTTATAACAGACCATGGCTCAAGGGAGTGATTGTCAGACCAAAGAATTTAGAAATGTGCTTCCTTTTTGTGGTACCCAACTATTTCTGTTCATAGTATTTATGTTTTGTGGAGCTTGTCAACTGCTTCTTTAACTAGGCCTTGTGCGATAAAGCTCAAAGCCACTACTGGTCTATTTCTAGTTTCTATGAGATTTATACTTCGTTTTCTTGTTTGGGCTTGTTTCTTAGGATGATATCATTGCAAGGGTTGAAAATCGAATTGCAGTGTGGACTTTTCTCCCAAAAGGTATTTCTCATCAGTGTGGTACTGCAACTGTTTCCTTTTGCATTTCCCTGGCAATGCCCTTTTATTTGATCTTCTTTTAAACGTATTAGTTTGATTGTCTTCAGATTATAGCATGCCTTTACAGATTATGCATTACGGGGGTGAAGAAGCAGAGCATAAGTACATTTTTGGCAACAGATCTGCAATGTCGTCCAGTGAGCCTTTGATGGCCACAGTAGTTTTGTACCTCTCAGATTCCGCTAGCGGTGGCGAGATGCTCTTTCCTGAATCAAAGGTGAGAGGAAGTAAAATTTTTGTCCTTTTTATAACCTCATCTCCCCTTCTTTTGTCGCCCTTTTGTATTTTATAAAATTTTCTGTTTCACGATACTCAAACACATGTGGCCTTAATAAAGTACTCATGACTACCTTTTCAATGCCTCAGGTAAAGAGCAGCTTTTGGTCAGACCGGAGAAAGAAAAGCAACATTCTGAGACCAGTGAGAGGCAATGCAATTCTTTTTTTCTCTGTTCATCTTAATGCTTCTCCAGACAAGAGTAGCTACCATACCCGATCACCAGTACTTGATGGGAAATTGTGGGTTGCTACAAAATTCTTCTACTTAAGACCAACCACTGGGAATAAATACACAGTTGAGTCCATTGGAGACGACTGCATTGATGAAGATAAAAGCTGCCCCCAGTGGGCTGCCATTGGCGAATGCGAACGAAACGCTGTTTTCATGATTGGTTCTCCAGATTACTATGGAACATGTAGAAAAAGCTGCAATGCATGTTGA

mRNA sequence

ATGGATTCTCGTCTTAACTTTCTGCTTCTTTTAGTGACTGCATTTTCATTCTCAAGCTGCCTTGCACGAAGCAATTTGATTAGTGGTCGGAAGGGTTTAAGGGACCAGTTGGTCGACAGTGTTCCTTTGAGCTACTCAAATCATTCTGGAAGAATCGACCCTTCAAGAGTTGTCCAAGTCTCTTGGCGACCAAGGGTTTTCTTGTATAAAGGTTTTCTCTCAGATGCGGAATGTGATCACCTTATTTCTTTGGCTTCAAATTCAGAAGATAAACCTTCTGGGAACAGTGCTGGTTCTAGGAACACTGTCTCAACCAAATTCCTTAACAATTCAGGAGCCATTTTAAACACAACAGATGATATCATTGCAAGGGTTGAAAATCGAATTGCAGTGTGGACTTTTCTCCCAAAAGATTATAGCATGCCTTTACAGATTATGCATTACGGGGGTGAAGAAGCAGAGCATAAGTACATTTTTGGCAACAGATCTGCAATGTCGTCCAGTGAGCCTTTGATGGCCACAGTAGTTTTGTACCTCTCAGATTCCGCTAGCGGTGGCGAGATGCTCTTTCCTGAATCAAAGGTAAAGAGCAGCTTTTGGTCAGACCGGAGAAAGAAAAGCAACATTCTGAGACCAGTGAGAGGCAATGCAATTCTTTTTTTCTCTGTTCATCTTAATGCTTCTCCAGACAAGAGTAGCTACCATACCCGATCACCAGTACTTGATGGGAAATTGTGGGTTGCTACAAAATTCTTCTACTTAAGACCAACCACTGGGAATAAATACACAGTTGAGTCCATTGGAGACGACTGCATTGATGAAGATAAAAGCTGCCCCCAGTGGGCTGCCATTGGCGAATGCGAACGAAACGCTGTTTTCATGATTGGTTCTCCAGATTACTATGGAACATGTAGAAAAAGCTGCAATGCATGTTGA

Coding sequence (CDS)

ATGGATTCTCGTCTTAACTTTCTGCTTCTTTTAGTGACTGCATTTTCATTCTCAAGCTGCCTTGCACGAAGCAATTTGATTAGTGGTCGGAAGGGTTTAAGGGACCAGTTGGTCGACAGTGTTCCTTTGAGCTACTCAAATCATTCTGGAAGAATCGACCCTTCAAGAGTTGTCCAAGTCTCTTGGCGACCAAGGGTTTTCTTGTATAAAGGTTTTCTCTCAGATGCGGAATGTGATCACCTTATTTCTTTGGCTTCAAATTCAGAAGATAAACCTTCTGGGAACAGTGCTGGTTCTAGGAACACTGTCTCAACCAAATTCCTTAACAATTCAGGAGCCATTTTAAACACAACAGATGATATCATTGCAAGGGTTGAAAATCGAATTGCAGTGTGGACTTTTCTCCCAAAAGATTATAGCATGCCTTTACAGATTATGCATTACGGGGGTGAAGAAGCAGAGCATAAGTACATTTTTGGCAACAGATCTGCAATGTCGTCCAGTGAGCCTTTGATGGCCACAGTAGTTTTGTACCTCTCAGATTCCGCTAGCGGTGGCGAGATGCTCTTTCCTGAATCAAAGGTAAAGAGCAGCTTTTGGTCAGACCGGAGAAAGAAAAGCAACATTCTGAGACCAGTGAGAGGCAATGCAATTCTTTTTTTCTCTGTTCATCTTAATGCTTCTCCAGACAAGAGTAGCTACCATACCCGATCACCAGTACTTGATGGGAAATTGTGGGTTGCTACAAAATTCTTCTACTTAAGACCAACCACTGGGAATAAATACACAGTTGAGTCCATTGGAGACGACTGCATTGATGAAGATAAAAGCTGCCCCCAGTGGGCTGCCATTGGCGAATGCGAACGAAACGCTGTTTTCATGATTGGTTCTCCAGATTACTATGGAACATGTAGAAAAAGCTGCAATGCATGTTGA

Protein sequence

MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQVSWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDDIIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAEHKYIFGNRSAMSSSEPLMATVVLYLSDSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSPVLDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC

Homology

[Close]

BLAST of Tan0009550 vs. ExPASy Swiss-Prot
Match: Q8GXT7 (Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.8e-62
Identity = 135/310 (43.55%), Postives = 186/310 (60.00%), Query Frame = 0


Query: 7   FLLLLVTAFSFSSCLARSNLISGRKGLRDQLV----DSVPLSYSNHSGRIDPSRVVQVSW 66
           FL+L++T  S S           RK LRD+ +    D    SY   S  +DP+RV+Q+SW
Sbjct: 8   FLILMITMSSSSPPFCSG---GSRKELRDKEITSKSDDTQASYVLGSKFVDPTRVLQLSW 67

Query: 67  RPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDDII 126
            PRVFLY+GFLS+ ECDHLISL   + +  S ++ G                    D ++
Sbjct: 68  LPRVFLYRGFLSEEECDHLISLRKETTEVYSVDADGK----------------TQLDPVV 127

Query: 127 ARVENRIAVWTFLPKDYSMPLQIMHYGGEEAEHKY-IFGNRSAMSSSEPLMATVVLYLSD 186
           A +E +++ WTFLP +    +++  Y  E++  K   FG   +    E L+ATVVLYLS+
Sbjct: 128 AGIEEKVSAWTFLPGENGGSIKVRSYTSEKSGKKLDYFGEEPSSVLHESLLATVVLYLSN 187

Query: 187 SASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSPVL 246
           +  GGE+LFP S++K    +   +  NILRPV+GNAILFF+  LNAS D  S H R PV+
Sbjct: 188 TTQGGELLFPNSEMKPK--NSCLEGGNILRPVKGNAILFFTRLLNASLDGKSTHLRCPVV 247

Query: 247 DGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPDYY 306
            G+L VATK  Y +     +  +E  G +C DED++C +WA +GEC++N V+MIGSPDYY
Sbjct: 248 KGELLVATKLIYAK----KQARIEESG-ECSDEDENCGRWAKLGECKKNPVYMIGSPDYY 291

Query: 307 GTCRKSCNAC 312
           GTCRKSCNAC
Sbjct: 308 GTCRKSCNAC 291

[Close]

BLAST of Tan0009550 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.2e-54
Identity = 113/275 (41.09%), Postives = 165/275 (60.00%), Query Frame = 0


Query: 46  SNHSGRIDPSRVVQVSWRPRVFLYKGFLSDAECDHLISLASNSEDKPS-GNSAGSRNTVS 105
           S+ S  +DP+R+ Q+SW PR FLYKGFLSD ECDHLI LA    +K        S  +  
Sbjct: 21  SSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESED 80

Query: 106 TKFLNNSGAIL-NTTDDIIARVENRIAVWTFLPKDYSMPLQIMHY--GGEEAEHKYIFGN 165
           ++   +SG  L    DDI+A VE ++A WTFLP++    LQI+HY  G +   H   F +
Sbjct: 81  SEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYD 140

Query: 166 RSAMSSSEPLMATVVLYLSDSASGGEMLFPESK-----VKSSFWSDRRKKSNILRPVRGN 225
           + A+      +ATV++YLS+   GGE +FP  K     +K   WS   K+   ++P +G+
Sbjct: 141 KKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGD 200

Query: 226 AILFFSVHLNASPDKSSYHTRSPVLDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDK 285
           A+LFF++HLN + D +S H   PV++G+ W AT++ ++R + G K  V      C+D+ +
Sbjct: 201 ALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVR-SFGKKKLV------CVDDHE 260

Query: 286 SCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 312
           SC +WA  GECE+N ++M+GS    G CRKSC AC
Sbjct: 261 SCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Tan0009550 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.3e-53
Identity = 116/320 (36.25%), Postives = 184/320 (57.50%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSN-LISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQ 60
           MDSR+     L   F+     +  N  ++     RD  V  + +  S  S   DP+RV Q
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSV--IKMKTSASSFGFDPTRVTQ 60

Query: 61  VSWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILN-TT 120
           +SW PRVFLY+GFLSD ECDH I LA    +K       S  +V ++   +SG  L+   
Sbjct: 61  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 120

Query: 121 DDIIARVENRIAVWTFLPKDYSMPLQIMHY--GGEEAEHKYIFGNRSAMSSSEPLMATVV 180
           DDI++ VE ++A WTFLP++    +QI+HY  G +   H   F +++ +      +ATV+
Sbjct: 121 DDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVL 180

Query: 181 LYLSDSASGGEMLFP-----ESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDK 240
           +YLS+   GGE +FP      +++K   W++  K+   ++P +G+A+LFF++H NA+ D 
Sbjct: 181 MYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDS 240

Query: 241 SSYHTRSPVLDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNA 300
           +S H   PV++G+ W AT++ +++ +    +  +S    C+DE+ SC +WA  GEC++N 
Sbjct: 241 NSLHGSCPVVEGEKWSATRWIHVK-SFERAFNKQS---GCMDENVSCEKWAKAGECQKNP 300

Query: 301 VFMIGSPDYYGTCRKSCNAC 312
            +M+GS   +G CRKSC AC
Sbjct: 301 TYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Tan0009550 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.8e-46
Identity = 103/286 (36.01%), Postives = 166/286 (58.04%), Query Frame = 0


Query: 37  LVDSVPLSYSNHSGRIDPSRVVQVSWRPRVFLYKGFLSDAECDHLISLA-SNSEDKPSGN 96
           L+ S     S+ S  I+PS+V QVS +PR F+Y+GFL+D ECDHLISLA  N +     +
Sbjct: 18  LLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVAD 77

Query: 97  SAGSRNTVSTKFLNNSGAILNTTDDIIARVENRIAVWTFLPKDYSMPLQIMHY--GGEEA 156
           +    + VS    ++   I    D I++ +E++++ WTFLPK+    LQ++ Y  G +  
Sbjct: 78  NDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYD 137

Query: 157 EHKYIFGNRSAMSSSEPLMATVVLYLSDSASGGEMLFPESKVKS--------SFWSDRRK 216
            H   F ++  ++     +ATV+LYLS+   GGE +FP+++  S           SD  K
Sbjct: 138 AHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAK 197

Query: 217 KSNILRPVRGNAILFFSVHLNASPDKSSYHTRSPVLDGKLWVATKFFYLRPTTGNKYTVE 276
           K   ++P +GNA+LFF++  +A PD  S H   PV++G+ W ATK+ ++     +   + 
Sbjct: 198 KGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHV----DSFDKIL 257

Query: 277 SIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 312
           +   +C D ++SC +WA +GEC +N  +M+G+P+  G CR+SC AC
Sbjct: 258 THDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of Tan0009550 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.8e-45
Identity = 99/302 (32.78%), Postives = 174/302 (57.62%), Query Frame = 0


Query: 21  LARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQVSWRPRVFLYKGFLSDAECDH 80
           +AR  L+     +   L+ S     S+ S  ++PS+V QVS +PR F+Y+GFL++ ECDH
Sbjct: 1   MARRGLLISFFAIFSVLLQSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDH 60

Query: 81  LISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNT-TDDIIARVENRIAVWTFLPKDY 140
           ++SLA  S  + +     S  +  ++   +SG  ++   D I++ +E++I+ WTFLPK+ 
Sbjct: 61  MVSLAKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKEN 120

Query: 141 SMPLQIMHY--GGEEAEHKYIFGNRSAMSSSEPLMATVVLYLSDSASGGEMLFPESKVKS 200
              +Q++ Y  G +   H   F ++  +      MAT+++YLS+   GGE +FP++++ S
Sbjct: 121 GEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPS 180

Query: 201 --------SFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSPVLDGKLWVAT 260
                      SD  K+   ++P +G+A+LFF++H +A PD  S H   PV++G+ W AT
Sbjct: 181 RRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSAT 240

Query: 261 KFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCN 312
           K+ ++     +   + +   +C D ++SC +WA +GEC +N  +M+G+ +  G CR+SC 
Sbjct: 241 KWIHV----DSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCK 298

[Close]

BLAST of Tan0009550 vs. NCBI nr
Match: XP_038906497.1 (probable prolyl 4-hydroxylase 12 [Benincasa hispida])

HSP 1 Score: 563.1 bits (1450), Expect = 1.4e-156
Identity = 275/311 (88.42%), Postives = 289/311 (92.93%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRLNFLLLL TAFSFS+CLA+SNLISGRKGLRDQLVD  PLSYSNHSGRIDPSRVVQV
Sbjct: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDR-PLSYSNHSGRIDPSRVVQV 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SW+PRVFLYKGFLSD ECDHLISLASNSED PSGNSAGS NTVSTK LN+SG ILNT+DD
Sbjct: 61  SWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAEHKYIFGNRSAMSSSEPLMATVVLYLS 180
           IIAR+EN+IAVWTFLPKD+ MP QIM Y GEEAEHKY +GN SAMSSSEPLMATVVLYLS
Sbjct: 121 IIARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAMSSSEPLMATVVLYLS 180

Query: 181 DSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSPV 240
           DSA GGEMLFPESKVKS FWSDRRKK+N LRPV+GNAILFFSVHLNASPDKSSYHTRSP+
Sbjct: 181 DSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPI 240

Query: 241 LDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPDY 300
           L+G+LWVATKFFYLRPTTGNK TVES  D CIDEDKSCPQWAAIGECERN VFMIGSPDY
Sbjct: 241 LNGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDY 300

Query: 301 YGTCRKSCNAC 312
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 310

[Close]

BLAST of Tan0009550 vs. NCBI nr
Match: XP_022159842.1 (probable prolyl 4-hydroxylase 12 [Momordica charantia])

HSP 1 Score: 555.4 bits (1430), Expect = 3.0e-154
Identity = 271/311 (87.14%), Postives = 287/311 (92.28%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRL  LLLL TA SF SCLA+SNLISGRKGLRDQL++SVPLSYSNHSGRIDPSRVVQV
Sbjct: 1   MDSRLPVLLLLATAISFLSCLAQSNLISGRKGLRDQLIESVPLSYSNHSGRIDPSRVVQV 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SWRPRVFLYKGFLSD ECDHLISLA++SEDKPSGNS  S NTV TK L +SGAILNTTDD
Sbjct: 61  SWRPRVFLYKGFLSDEECDHLISLATSSEDKPSGNSTDSGNTVPTKILKSSGAILNTTDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAEHKYIFGNRSAMSSSEPLMATVVLYLS 180
           IIAR+ENRIAVWTFLPKDYSMPLQI+ YGGEEAEHKY+FGNRSAM SSEPLMATVVLYLS
Sbjct: 121 IIARIENRIAVWTFLPKDYSMPLQILQYGGEEAEHKYVFGNRSAMLSSEPLMATVVLYLS 180

Query: 181 DSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSPV 240
           DSASGGEM FPESKVKS FWSDRRKK+NILRPV+GNA+L FSVHLNASPDKSS HTRSP+
Sbjct: 181 DSASGGEMRFPESKVKSRFWSDRRKKNNILRPVKGNAVLIFSVHLNASPDKSSSHTRSPI 240

Query: 241 LDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPDY 300
           LDG+LW+ATKFFYLRP TGNK+T E  G DC DEDKSCPQWAAIGECERNAVFMIGSPDY
Sbjct: 241 LDGELWIATKFFYLRPITGNKHTDEPDG-DCNDEDKSCPQWAAIGECERNAVFMIGSPDY 300

Query: 301 YGTCRKSCNAC 312
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 310

[Close]

BLAST of Tan0009550 vs. NCBI nr
Match: KAG6579383.1 (putative prolyl 4-hydroxylase 12, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016863.1 putative prolyl 4-hydroxylase 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 541.2 bits (1393), Expect = 5.9e-150
Identity = 264/314 (84.08%), Postives = 288/314 (91.72%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRLNFLLLL  AFSFSSCLA+SN ISGRKGLRDQ+V+S  LSYSNHS RIDPSRVVQ+
Sbjct: 1   MDSRLNFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SW+PR FLYKGFLSD ECDHLI+LASNSEDKPS N+AGSRNTVSTKFL NSGAILNTTDD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAE-HKYIFGNRSAMSSSEPLMATVVLYL 180
           IIAR+ENRIAVWTFLPKD+SMP QIM YGGEEA  HKY FGNRSAM SSEPLMATVVLYL
Sbjct: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 181 SDSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSP 240
           SDSASGGE+LFP SKVK  FWSD+RKK+N LRPV+GNA+LFFSVHLNASPDKS YH+R+P
Sbjct: 181 SDSASGGEILFPVSKVKRRFWSDQRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 241 VLDGKLWVATKFFYLRP-TTGNKYTVES-IGDDCIDEDKSCPQWAAIGECERNAVFMIGS 300
           +LDGKLWVATKFFY+RP  TGN++ VES + DDCIDED+SCP+WAAIGEC+RNAVFMIGS
Sbjct: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300

Query: 301 PDYYGTCRKSCNAC 312
           PDYYGTCRKSCNAC
Sbjct: 301 PDYYGTCRKSCNAC 314

[Close]

BLAST of Tan0009550 vs. NCBI nr
Match: XP_022922237.1 (probable prolyl 4-hydroxylase 12 [Cucurbita moschata])

HSP 1 Score: 538.5 bits (1386), Expect = 3.8e-149
Identity = 263/314 (83.76%), Postives = 286/314 (91.08%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRL FLLLL  AFSFSSCLA+SN ISGRKGLRDQ+V+S  LSYSNHS RIDPSRVVQ+
Sbjct: 1   MDSRLTFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SW+PR FLYKGFLSD ECDHLI+LASNSEDKPS N+AGSRNTVSTKFL NSGAILNTTDD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAE-HKYIFGNRSAMSSSEPLMATVVLYL 180
           II R+ENRIAVWTFLPKD+SMP QIM YGGEEA  HKY FGNRSAM SSEPLMATVVLYL
Sbjct: 121 IIGRIENRIAVWTFLPKDHSMPFQIMKYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 181 SDSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSP 240
           SDSASGGE+LFP SKVK  FWSDRRKK+N LRPV+GNA+LFFSVHLNASPDKS YH+R+P
Sbjct: 181 SDSASGGEILFPVSKVKRRFWSDRRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 241 VLDGKLWVATKFFYLRP-TTGNKYTVES-IGDDCIDEDKSCPQWAAIGECERNAVFMIGS 300
           +LDGKLWVATKFFY+RP  TGN++ VES + DDCIDED+SCP+WAAIGEC+RNAVFMIGS
Sbjct: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300

Query: 301 PDYYGTCRKSCNAC 312
           PDYYGTCRKSCNAC
Sbjct: 301 PDYYGTCRKSCNAC 314

[Close]

BLAST of Tan0009550 vs. NCBI nr
Match: XP_023549812.1 (probable prolyl 4-hydroxylase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 537.7 bits (1384), Expect = 6.5e-149
Identity = 261/314 (83.12%), Postives = 287/314 (91.40%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRLNFLLL   AFSFSSCLA+SN +SGRKGLRDQ+V+S  LSYSNH  RIDPSRVVQ+
Sbjct: 1   MDSRLNFLLLFAAAFSFSSCLAQSNSVSGRKGLRDQMVNSGHLSYSNHFERIDPSRVVQI 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SW+PRVFLYKGFLSD ECDHLI+LASNSEDKPS ++AGSRNTVSTKFL NSGA+LNTTDD
Sbjct: 61  SWQPRVFLYKGFLSDEECDHLIALASNSEDKPSRSNAGSRNTVSTKFLGNSGAVLNTTDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAE-HKYIFGNRSAMSSSEPLMATVVLYL 180
           IIAR+ENRIAVWTFLPKD+SMP QIM YGGEEA  HKY FGNRSAM SSEPLMATVVLYL
Sbjct: 121 IIARIENRIAVWTFLPKDHSMPFQIMQYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 181 SDSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSP 240
           SDSASGGE+LFP SKVK  FWSDRRKK+N LRPV+GNA+LFFSVHLNASPDKS YH+R+P
Sbjct: 181 SDSASGGEILFPVSKVKRRFWSDRRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 241 VLDGKLWVATKFFYLRP-TTGNKYTVES-IGDDCIDEDKSCPQWAAIGECERNAVFMIGS 300
           +LDGKLWVATKFFY+RP  TGN++ VES + DDCIDED+SCP+WAAIGEC+RNAVFMIGS
Sbjct: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300

Query: 301 PDYYGTCRKSCNAC 312
           PDYYGTCRKSCNAC
Sbjct: 301 PDYYGTCRKSCNAC 314

[Close]

BLAST of Tan0009550 vs. ExPASy TrEMBL
Match: A0A6J1E0X9 (Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111026141 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.5e-154
Identity = 271/311 (87.14%), Postives = 287/311 (92.28%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRL  LLLL TA SF SCLA+SNLISGRKGLRDQL++SVPLSYSNHSGRIDPSRVVQV
Sbjct: 1   MDSRLPVLLLLATAISFLSCLAQSNLISGRKGLRDQLIESVPLSYSNHSGRIDPSRVVQV 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SWRPRVFLYKGFLSD ECDHLISLA++SEDKPSGNS  S NTV TK L +SGAILNTTDD
Sbjct: 61  SWRPRVFLYKGFLSDEECDHLISLATSSEDKPSGNSTDSGNTVPTKILKSSGAILNTTDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAEHKYIFGNRSAMSSSEPLMATVVLYLS 180
           IIAR+ENRIAVWTFLPKDYSMPLQI+ YGGEEAEHKY+FGNRSAM SSEPLMATVVLYLS
Sbjct: 121 IIARIENRIAVWTFLPKDYSMPLQILQYGGEEAEHKYVFGNRSAMLSSEPLMATVVLYLS 180

Query: 181 DSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSPV 240
           DSASGGEM FPESKVKS FWSDRRKK+NILRPV+GNA+L FSVHLNASPDKSS HTRSP+
Sbjct: 181 DSASGGEMRFPESKVKSRFWSDRRKKNNILRPVKGNAVLIFSVHLNASPDKSSSHTRSPI 240

Query: 241 LDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPDY 300
           LDG+LW+ATKFFYLRP TGNK+T E  G DC DEDKSCPQWAAIGECERNAVFMIGSPDY
Sbjct: 241 LDGELWIATKFFYLRPITGNKHTDEPDG-DCNDEDKSCPQWAAIGECERNAVFMIGSPDY 300

Query: 301 YGTCRKSCNAC 312
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 310

[Close]

BLAST of Tan0009550 vs. ExPASy TrEMBL
Match: A0A6J1E2P0 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111430280 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.8e-149
Identity = 263/314 (83.76%), Postives = 286/314 (91.08%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRL FLLLL  AFSFSSCLA+SN ISGRKGLRDQ+V+S  LSYSNHS RIDPSRVVQ+
Sbjct: 1   MDSRLTFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SW+PR FLYKGFLSD ECDHLI+LASNSEDKPS N+AGSRNTVSTKFL NSGAILNTTDD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAE-HKYIFGNRSAMSSSEPLMATVVLYL 180
           II R+ENRIAVWTFLPKD+SMP QIM YGGEEA  HKY FGNRSAM SSEPLMATVVLYL
Sbjct: 121 IIGRIENRIAVWTFLPKDHSMPFQIMKYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 181 SDSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSP 240
           SDSASGGE+LFP SKVK  FWSDRRKK+N LRPV+GNA+LFFSVHLNASPDKS YH+R+P
Sbjct: 181 SDSASGGEILFPVSKVKRRFWSDRRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 241 VLDGKLWVATKFFYLRP-TTGNKYTVES-IGDDCIDEDKSCPQWAAIGECERNAVFMIGS 300
           +LDGKLWVATKFFY+RP  TGN++ VES + DDCIDED+SCP+WAAIGEC+RNAVFMIGS
Sbjct: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300

Query: 301 PDYYGTCRKSCNAC 312
           PDYYGTCRKSCNAC
Sbjct: 301 PDYYGTCRKSCNAC 314

[Close]

BLAST of Tan0009550 vs. ExPASy TrEMBL
Match: A0A1S3AT39 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103482556 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 5.4e-149
Identity = 265/312 (84.94%), Postives = 280/312 (89.74%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRLNFLLL  TAFSFS+CLA+SNLISGRKGLRDQLVD  PLSYSN S RIDPSRVVQV
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDR-PLSYSNQSVRIDPSRVVQV 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SWRPRVFLYKGFLSD ECDHLISLASNSED PS NSAGS NTVST+ LN SG ILNTTDD
Sbjct: 61  SWRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGNTVSTELLNGSGVILNTTDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAEHKYIFGNRSAM-SSSEPLMATVVLYL 180
           IIAR+ENRIAVWT LPKD+ MP QIM Y GEEA+HKY +GNRSAM SSSEPLMATVVLYL
Sbjct: 121 IIARIENRIAVWTLLPKDHGMPFQIMQYRGEEAKHKYFYGNRSAMSSSSEPLMATVVLYL 180

Query: 181 SDSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSP 240
           SDSASGGEMLFPESKVKS FWS RRKK N LRPV+GNAILFFSVHLNASPDKSSYH R P
Sbjct: 181 SDSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRYP 240

Query: 241 VLDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPD 300
           + +G+LWVATKF YLRP TGNK+T++S  D CIDEDKSCPQWAAIGECERNAVFM+GSPD
Sbjct: 241 IRNGELWVATKFLYLRPPTGNKHTIDSNIDGCIDEDKSCPQWAAIGECERNAVFMVGSPD 300

Query: 301 YYGTCRKSCNAC 312
           YYGTCRKSCNAC
Sbjct: 301 YYGTCRKSCNAC 311

[Close]

BLAST of Tan0009550 vs. ExPASy TrEMBL
Match: A0A5A7TKX1 (Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00060 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.0e-148
Identity = 264/312 (84.62%), Postives = 280/312 (89.74%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRLNFLLL  TAFSFS+CLA+SNLISGRKGLRDQLVD  PLSYSN S RIDPSRVVQV
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDR-PLSYSNQSVRIDPSRVVQV 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SWRPRVFLYKGFLSD ECDHLISLASNS+D PS NSAGS NTVST+ LN SG ILNTTDD
Sbjct: 61  SWRPRVFLYKGFLSDDECDHLISLASNSKDNPSRNSAGSGNTVSTELLNGSGVILNTTDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAEHKYIFGNRSAM-SSSEPLMATVVLYL 180
           IIAR+ENRIAVWT LPKD+ MP QIM Y GEEA+HKY +GNRSAM SSSEPLMATVVLYL
Sbjct: 121 IIARIENRIAVWTLLPKDHGMPFQIMQYRGEEAKHKYFYGNRSAMSSSSEPLMATVVLYL 180

Query: 181 SDSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSP 240
           SDSASGGEMLFPESKVKS FWS RRKK N LRPV+GNAILFFSVHLNASPDKSSYH R P
Sbjct: 181 SDSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRYP 240

Query: 241 VLDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPD 300
           + +G+LWVATKF YLRP TGNK+T++S  D CIDEDKSCPQWAAIGECERNAVFM+GSPD
Sbjct: 241 IRNGELWVATKFLYLRPPTGNKHTIDSNIDGCIDEDKSCPQWAAIGECERNAVFMVGSPD 300

Query: 301 YYGTCRKSCNAC 312
           YYGTCRKSCNAC
Sbjct: 301 YYGTCRKSCNAC 311

[Close]

BLAST of Tan0009550 vs. ExPASy TrEMBL
Match: A0A6J1IBS3 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111471522 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.5e-148
Identity = 261/314 (83.12%), Postives = 285/314 (90.76%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSNLISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQV 60
           MDSRLNFLLLL  AFSF SCLA+SN ISGRKGLRDQ+V+S  LSYSNHS RIDPSRVVQ+
Sbjct: 1   MDSRLNFLLLLAAAFSFPSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60

Query: 61  SWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDD 120
           SW+PR FLYKGFLSD ECDHLI+LASNSEDKPS N+AGSRNTVSTKFL NSGAILNTTDD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120

Query: 121 IIARVENRIAVWTFLPKDYSMPLQIMHYGGEEAE-HKYIFGNRSAMSSSEPLMATVVLYL 180
           IIAR+ENRIAVW FLPKD+SMP QIM YGGEEA   KY FGNRSAM SSEPLMATVVLYL
Sbjct: 121 IIARIENRIAVWLFLPKDHSMPFQIMQYGGEEAAGRKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 181 SDSASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSP 240
           SDSA+GGE+LFP SKVK  FWSDRRKK+N LRPV+GNA+LFFSVHLNASPDKS YH+R+P
Sbjct: 181 SDSANGGEILFPVSKVKRRFWSDRRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 241 VLDGKLWVATKFFYLRP-TTGNKYTVES-IGDDCIDEDKSCPQWAAIGECERNAVFMIGS 300
           +LDGKLWVATKFFY+RP  TGN++ VES + DDCIDED+SCP+WAAIGEC+RNAVFMIGS
Sbjct: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300

Query: 301 PDYYGTCRKSCNAC 312
           PDYYGTCRKSCNAC
Sbjct: 301 PDYYGTCRKSCNAC 314

[Close]

BLAST of Tan0009550 vs. TAIR 10
Match: AT4G25600.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 240.4 bits (612), Expect = 2.0e-63
Identity = 135/310 (43.55%), Postives = 186/310 (60.00%), Query Frame = 0


Query: 7   FLLLLVTAFSFSSCLARSNLISGRKGLRDQLV----DSVPLSYSNHSGRIDPSRVVQVSW 66
           FL+L++T  S S           RK LRD+ +    D    SY   S  +DP+RV+Q+SW
Sbjct: 8   FLILMITMSSSSPPFCSG---GSRKELRDKEITSKSDDTQASYVLGSKFVDPTRVLQLSW 67

Query: 67  RPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILNTTDDII 126
            PRVFLY+GFLS+ ECDHLISL   + +  S ++ G                    D ++
Sbjct: 68  LPRVFLYRGFLSEEECDHLISLRKETTEVYSVDADGK----------------TQLDPVV 127

Query: 127 ARVENRIAVWTFLPKDYSMPLQIMHYGGEEAEHKY-IFGNRSAMSSSEPLMATVVLYLSD 186
           A +E +++ WTFLP +    +++  Y  E++  K   FG   +    E L+ATVVLYLS+
Sbjct: 128 AGIEEKVSAWTFLPGENGGSIKVRSYTSEKSGKKLDYFGEEPSSVLHESLLATVVLYLSN 187

Query: 187 SASGGEMLFPESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDKSSYHTRSPVL 246
           +  GGE+LFP S++K    +   +  NILRPV+GNAILFF+  LNAS D  S H R PV+
Sbjct: 188 TTQGGELLFPNSEMKPK--NSCLEGGNILRPVKGNAILFFTRLLNASLDGKSTHLRCPVV 247

Query: 247 DGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPDYY 306
            G+L VATK  Y +     +  +E  G +C DED++C +WA +GEC++N V+MIGSPDYY
Sbjct: 248 KGELLVATKLIYAK----KQARIEESG-ECSDEDENCGRWAKLGECKKNPVYMIGSPDYY 291

Query: 307 GTCRKSCNAC 312
           GTCRKSCNAC
Sbjct: 308 GTCRKSCNAC 291

[Close]

BLAST of Tan0009550 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-55
Identity = 113/275 (41.09%), Postives = 165/275 (60.00%), Query Frame = 0


Query: 46  SNHSGRIDPSRVVQVSWRPRVFLYKGFLSDAECDHLISLASNSEDKPS-GNSAGSRNTVS 105
           S+ S  +DP+R+ Q+SW PR FLYKGFLSD ECDHLI LA    +K        S  +  
Sbjct: 21  SSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESED 80

Query: 106 TKFLNNSGAIL-NTTDDIIARVENRIAVWTFLPKDYSMPLQIMHY--GGEEAEHKYIFGN 165
           ++   +SG  L    DDI+A VE ++A WTFLP++    LQI+HY  G +   H   F +
Sbjct: 81  SEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFYD 140

Query: 166 RSAMSSSEPLMATVVLYLSDSASGGEMLFPESK-----VKSSFWSDRRKKSNILRPVRGN 225
           + A+      +ATV++YLS+   GGE +FP  K     +K   WS   K+   ++P +G+
Sbjct: 141 KKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKGD 200

Query: 226 AILFFSVHLNASPDKSSYHTRSPVLDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDK 285
           A+LFF++HLN + D +S H   PV++G+ W AT++ ++R + G K  V      C+D+ +
Sbjct: 201 ALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVR-SFGKKKLV------CVDDHE 260

Query: 286 SCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 312
           SC +WA  GECE+N ++M+GS    G CRKSC AC
Sbjct: 261 SCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of Tan0009550 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 209.5 bits (532), Expect = 3.8e-54
Identity = 116/320 (36.25%), Postives = 184/320 (57.50%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSN-LISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQ 60
           MDSR+     L   F+     +  N  ++     RD  V  + +  S  S   DP+RV Q
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSV--IKMKTSASSFGFDPTRVTQ 60

Query: 61  VSWRPRVFLYKGFLSDAECDHLISLASNSEDKPSGNSAGSRNTVSTKFLNNSGAILN-TT 120
           +SW PRVFLY+GFLSD ECDH I LA    +K       S  +V ++   +SG  L+   
Sbjct: 61  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 120

Query: 121 DDIIARVENRIAVWTFLPKDYSMPLQIMHY--GGEEAEHKYIFGNRSAMSSSEPLMATVV 180
           DDI++ VE ++A WTFLP++    +QI+HY  G +   H   F +++ +      +ATV+
Sbjct: 121 DDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVL 180

Query: 181 LYLSDSASGGEMLFP-----ESKVKSSFWSDRRKKSNILRPVRGNAILFFSVHLNASPDK 240
           +YLS+   GGE +FP      +++K   W++  K+   ++P +G+A+LFF++H NA+ D 
Sbjct: 181 MYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDS 240

Query: 241 SSYHTRSPVLDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQWAAIGECERNA 300
           +S H   PV++G+ W AT++ +++ +    +  +S    C+DE+ SC +WA  GEC++N 
Sbjct: 241 NSLHGSCPVVEGEKWSATRWIHVK-SFERAFNKQS---GCMDENVSCEKWAKAGECQKNP 300

Query: 301 VFMIGSPDYYGTCRKSCNAC 312
            +M+GS   +G CRKSC AC
Sbjct: 301 TYMVGSDKDHGYCRKSCKAC 314

[Close]

BLAST of Tan0009550 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 208.0 bits (528), Expect = 1.1e-53
Identity = 120/330 (36.36%), Postives = 188/330 (56.97%), Query Frame = 0


Query: 1   MDSRLNFLLLLVTAFSFSSCLARSN-LISGRKGLRDQLVDSVPLSYSNHSGRIDPSRVVQ 60
           MDSR+     L   F+     +  N  ++     RD  V  + +  S  S   DP+RV Q
Sbjct: 1   MDSRIFLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSV--IKMKTSASSFGFDPTRVTQ 60

Query: 61  VSWRPRVFLYKGFLSDAECDHLISLA------SNSEDKPSGNSAGSRNTV-----STKFL 120
           +SW PRVFLY+GFLSD ECDH I LA      S   D  SG S  S ++V     S+ F+
Sbjct: 61  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSSFI 120

Query: 121 NNSGAILNTTDDIIARVENRIAVWTFLPKDYSMPLQIMHY--GGEEAEHKYIFGNRSAMS 180
            N  ++    DDI++ VE ++A WTFLP++    +QI+HY  G +   H   F +++ + 
Sbjct: 121 ANMDSL--EIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLE 180

Query: 181 SSEPLMATVVLYLSDSASGGEMLFP-----ESKVKSSFWSDRRKKSNILRPVRGNAILFF 240
                +ATV++YLS+   GGE +FP      +++K   W++  K+   ++P +G+A+LFF
Sbjct: 181 LGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFF 240

Query: 241 SVHLNASPDKSSYHTRSPVLDGKLWVATKFFYLRPTTGNKYTVESIGDDCIDEDKSCPQW 300
           ++H NA+ D +S H   PV++G+ W AT++ +++ +    +  +S    C+DE+ SC +W
Sbjct: 241 NLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVK-SFERAFNKQS---GCMDENVSCEKW 300

Query: 301 AAIGECERNAVFMIGSPDYYGTCRKSCNAC 312
           A  GEC++N  +M+GS   +G CRKSC AC
Sbjct: 301 AKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

[Close]

BLAST of Tan0009550 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-47
Identity = 103/286 (36.01%), Postives = 166/286 (58.04%), Query Frame = 0


Query: 37  LVDSVPLSYSNHSGRIDPSRVVQVSWRPRVFLYKGFLSDAECDHLISLA-SNSEDKPSGN 96
           L+ S     S+ S  I+PS+V QVS +PR F+Y+GFL+D ECDHLISLA  N +     +
Sbjct: 18  LLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVAD 77

Query: 97  SAGSRNTVSTKFLNNSGAILNTTDDIIARVENRIAVWTFLPKDYSMPLQIMHY--GGEEA 156
           +    + VS    ++   I    D I++ +E++++ WTFLPK+    LQ++ Y  G +  
Sbjct: 78  NDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYD 137

Query: 157 EHKYIFGNRSAMSSSEPLMATVVLYLSDSASGGEMLFPESKVKS--------SFWSDRRK 216
            H   F ++  ++     +ATV+LYLS+   GGE +FP+++  S           SD  K
Sbjct: 138 AHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAK 197

Query: 217 KSNILRPVRGNAILFFSVHLNASPDKSSYHTRSPVLDGKLWVATKFFYLRPTTGNKYTVE 276
           K   ++P +GNA+LFF++  +A PD  S H   PV++G+ W ATK+ ++     +   + 
Sbjct: 198 KGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHV----DSFDKIL 257

Query: 277 SIGDDCIDEDKSCPQWAAIGECERNAVFMIGSPDYYGTCRKSCNAC 312
           +   +C D ++SC +WA +GEC +N  +M+G+P+  G CR+SC AC
Sbjct: 258 THDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

The following BLAST results are available for this feature:

BLAST of Tan0009550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GXT7	2.8e-62	43.55	Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 S...	[more]
F4J0A8	2.2e-54	41.09	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=...	[more]
Q8L970	5.3e-53	36.25	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]
F4JAU3	2.8e-46	36.01	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]
Q8LAN3	1.8e-45	32.78	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]

BLAST of Tan0009550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906497.1	1.4e-156	88.42	probable prolyl 4-hydroxylase 12 [Benincasa hispida]	[more]
XP_022159842.1	3.0e-154	87.14	probable prolyl 4-hydroxylase 12 [Momordica charantia]	[more]
KAG6579383.1	5.9e-150	84.08	putative prolyl 4-hydroxylase 12, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022922237.1	3.8e-149	83.76	probable prolyl 4-hydroxylase 12 [Cucurbita moschata]	[more]
XP_023549812.1	6.5e-149	83.12	probable prolyl 4-hydroxylase 12 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Tan0009550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0X9	1.5e-154	87.14	Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111026141...	[more]
A0A6J1E2P0	1.8e-149	83.76	Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111430280 ...	[more]
A0A1S3AT39	5.4e-149	84.94	Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103482556 PE=3 S...	[more]
A0A5A7TKX1	2.0e-148	84.62	Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A6J1IBS3	3.5e-148	83.12	Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111471522 PE...	[more]

BLAST of Tan0009550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25600.1	2.0e-63	43.55	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	1.5e-55	41.09	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.1	3.8e-54	36.25	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.2	1.1e-53	36.36	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G06300.1	2.0e-47	36.01	P4H isoform 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 270..311 e-value: 3.7E-4 score: 29.8
IPR003582	ShKT domain	PROSITE	PS51670	SHKT	coord: 271..311 score: 8.52967
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 64..253 e-value: 2.1E-17 score: 73.8
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 51..253 e-value: 1.4E-38 score: 134.7
None	No IPR available	PANTHER	PTHR10869:SF102	PROLYL 4-HYDROXYLASE 12-RELATED	coord: 1..311
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..20 score: 5.0
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 167..253 e-value: 4.1E-6 score: 27.6
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 1..311

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0009550.1	Tan0009550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019511	peptidyl-proline hydroxylation
cellular_component	GO:0005789	endoplasmic reticulum membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen