Tan0009395 (gene) Snake gourd v1

BLAST of Tan0009395 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 1.5e-242
Identity = 423/748 (56.55%), Postives = 541/748 (72.33%), Query Frame = 0

Query: 10  SSYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAV 69
           SSY+VY G HS     +        E+ Y+ LG+   S+  A ++I YSY ++INGFAA 
Sbjct: 30  SSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAH 89

Query: 70  LDEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTII 129
           LD   A  ++K+P VVSVF NK  KLHTTRSW FLG+E +  +PS+SIW+ ARFGEDTII
Sbjct: 90  LDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTII 149

Query: 130 GNLDTGAWPESKSFNDAGYGPVPSRWRGACEG--GSNFQCNKKLIGARYFYQGFAMTHGP 189
            NLDTG WPESKSF D G GP+PSRW+G C+    + F CN+KLIGARYF +G+A   G 
Sbjct: 150 ANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGH 209

Query: 190 LNISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNK 249
           LN S  + RD +GHGSHTLSTA G+FVPGV++FG GNGTAKGGSP+AR+AAYKVCWP  K
Sbjct: 210 LNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVK 269

Query: 250 DGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGN 309
              C+D+D+LA F+AAI DG DV+SVSLG     F  DS++IG+FHA ++ IVVVCSAGN
Sbjct: 270 GNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGN 329

Query: 310 DGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINA 369
            GP+  TVSNV+PW  TVGAS++DR+F+S + LGN KH+KG SLSS  LP  KFYP++ +
Sbjct: 330 SGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMAS 389

Query: 370 VEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILAND 429
           V AKA NAS   AQLC+ GSLDP K KGKI+VCLRG NGRV+KG  V   GG+GM+L N 
Sbjct: 390 VNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENT 449

Query: 430 EKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFS 489
              G+ ++ DPH+LPA+ ++  D  ++++YI+ TK P+A +T  RT+LG+KP+PVMASFS
Sbjct: 450 YVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFS 509

Query: 490 SRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISG 549
           S+GP+ +   ++KPDITAPGVS+IA+ T  V+ T+  FD RR+ FN  SGTSMSCPHISG
Sbjct: 510 SKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISG 569

Query: 550 VVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNN 609
           + GLLKT YP+WSPAAI+SAIMTTA   D  ++   + ++T + K TPF +GAGHV PN 
Sbjct: 570 IAGLLKTRYPSWSPAAIRSAIMTTATIMD--DIPGPIQNATNM-KATPFSFGAGHVQPNL 629

Query: 610 AMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAE-AFAITDLNYPSISVPKLQ 669
           A++PGLVYD  I DYLNFLC+ GYN+  +  FS N F C+    ++ +LNYPSI+VP L 
Sbjct: 630 AVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNL- 689

Query: 670 IGAPFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSK 729
             +  T++R VKNVG     Y  +V  P+GV V V+P++L F  VGE+K FK++   +  
Sbjct: 690 TSSKVTVSRTVKNVG-RPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKG 749

Query: 730 LQRQGYVFGTLVWSDGKHFVRSPIAVKL 755
              +GYVFG LVWSD KH VRSPI VKL
Sbjct: 750 NVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Tan0009395 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 1.0e-235
Identity = 420/746 (56.30%), Postives = 517/746 (69.30%), Query Frame = 0

Query: 11  SYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVL 70
           SYIVYLG+H+     S         S    L + + S   AKE+I YSY R+INGFAA+L
Sbjct: 41  SYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAIL 100

Query: 71  DEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIG 130
           DE EA  +AK+P+VVSVF NK RKLHTT SW F+ +  +G +  +S+W  A +GEDTII 
Sbjct: 101 DENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIA 160

Query: 131 NLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGF-AMTHGPLN 190
           NLDTG WPESKSF+D GYG VP+RW+G C    +  CN+KLIGARYF +G+ A T  P N
Sbjct: 161 NLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPSN 220

Query: 191 ISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNKDG 250
            S  T RDH+GHGSHTLSTA GNFVPG NVFG GNGTA GGSPKAR+AAYKVCWP     
Sbjct: 221 ASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGA 280

Query: 251 GCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGNDG 310
            CFD+DILA  EAAI DGVDVLS S+G  A ++  D ++IG+FHA++ G+ VVCSAGN G
Sbjct: 281 ECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSG 340

Query: 311 PSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINAVE 370
           P  GTVSNV+PW+ TVGASS+DR+F ++V L N + FKG+SL SK LP  K Y LI+A +
Sbjct: 341 PKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSL-SKPLPEEKMYSLISAAD 400

Query: 371 AKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILANDEK 430
           A   N + T A LC++GSLDP K KGKI+VCLRGDN RVDKG +    G  GM+L ND+ 
Sbjct: 401 ANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKA 460

Query: 431 DGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFSSR 490
            G+ I+ D H+LPAS + Y DG ++  Y++STK P  ++      L  KP+P MASFSSR
Sbjct: 461 SGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSR 520

Query: 491 GPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISGVV 550
           GPN IT  ++KPDITAPGV+IIA+ TE    TDL  D RR PFN ESGTSMSCPHISGVV
Sbjct: 521 GPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVV 580

Query: 551 GLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNNAM 610
           GLLKTL+P WSPAAI+SAIMTT++TR+N     +        K  PF YG+GHV PN A 
Sbjct: 581 GLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMV---DESFKKANPFSYGSGHVQPNKAA 640

Query: 611 DPGLVYDTTIDDYLNFLCARGYNSLALKKFSNN-QFVCAEAFAITDLNYPSISVPKLQIG 670
            PGLVYD T  DYL+FLCA GYN+  ++ F+ + Q+ C +   + D NYPSI+VP L   
Sbjct: 641 HPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLT-- 700

Query: 671 APFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSKLQ 730
              T+ R++KNVG  A TY AR + P GV V+VEP  L FN  GE K F++  +    + 
Sbjct: 701 GSITVTRKLKNVGPPA-TYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLR-PLPVT 760

Query: 731 RQGYVFGTLVWSDGKHFVRSPIAVKL 755
             GYVFG L W+D  H+VRSPI V+L
Sbjct: 761 PSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Tan0009395 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 743.8 bits (1919), Expect = 1.9e-213
Identity = 397/765 (51.90%), Postives = 515/765 (67.32%), Query Frame = 0

Query: 12  YIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVLD 71
           YIVY+G HS G +P+  D +LAT+S Y++LG++  S+  AKE+IIYSYNR+INGFAA+L+
Sbjct: 32  YIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFAALLE 91

Query: 72  EKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIGN 131
           E+EA  +AKNPNVVSVF +KE KLHTTRSW FLG+   G    NS W+  RFGE+TIIGN
Sbjct: 92  EEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QNSAWQKGRFGENTIIGN 151

Query: 132 LDTGAWPESKSFNDAGYGPVPSRWRGA-CE-----GGSNFQCNKKLIGARYFYQGFAMTH 191
           +DTG WPES+SF+D GYG VPS+WRG  C+     G     CN+KLIGARY+ + F   +
Sbjct: 152 IDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAHN 211

Query: 192 GPLNISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPS 251
           G L+  LHTARD  GHG+HTLSTAGGNFVPG  VF  GNGTAKGGSP+AR+AAYKVCW  
Sbjct: 212 GQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCWSL 271

Query: 252 NKDGGCFDSDILAGFEAAISDGVDVLSVSLGAA----AQEFAYDSLSIGAFHAIQQGIVV 311
                C+ +D+LA  + AI DGVDV++VS G +    A+    D +SIGAFHAI + I++
Sbjct: 272 TDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNILL 331

Query: 312 VCSAGNDGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKF 371
           V SAGNDGP+PGTV+NV+PW+FT+ AS++DRDFSS + + N+   +G+SL    LP  + 
Sbjct: 332 VASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINNQL-IEGASL-FVNLPPNQA 391

Query: 372 YPLINAVEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLR-GDNGRVDKGFEVLRVGGV 431
           + LI + +AK  NA+   AQLC  G+LD TK  GKI++C R G    V +G E L  G  
Sbjct: 392 FSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGAR 451

Query: 432 GMILANDEKDGSTIMVDPHLLPA---------SHVSYVDGLSIAQYINSTKT-PVAFLTH 491
           GMIL N  ++G T+  +PH+            S    V   +I    +  KT     ++ 
Sbjct: 452 GMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSR 511

Query: 492 VRTELGIKPSPVMASFSSRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRR- 551
            RT  G KP+PVMASFSSRGPN I  S++KPD+TAPGV+I+A+ +E  +A+ L  D RR 
Sbjct: 512 ARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRG 571

Query: 552 VPFNVESGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTK 611
             FNV  GTSMSCPH SG+ GLLKT +P+WSPAAIKSAIMTTA T DNTN    + D+  
Sbjct: 572 FKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRP--IQDAFD 631

Query: 612 VGKGTPFDYGAGHVHPNNAMDPGLVYDTTIDDYLNFLCARGYNSLALKKFS-NNQFVCAE 671
                 F YG+GHV P+ A++PGLVYD ++ DYLNFLCA GY+   +   + N  F+C+ 
Sbjct: 632 KTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSG 691

Query: 672 AFAITDLNYPSISVPKLQIGAPFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQF 731
           + ++ DLNYPSI++P L++  P TI R V NVG    TY    + P G ++ V P +L F
Sbjct: 692 SHSVNDLNYPSITLPNLRL-KPVTIARTVTNVG-PPSTYTVSTRSPNGYSIAVVPPSLTF 751

Query: 732 NSVGEEKAFKLVFQCNSKLQRQGYVFGTLVWSDGKHFVRSPIAVK 754
             +GE K FK++ Q +S   R+ Y FG L W+DGKH VRSPI VK
Sbjct: 752 TKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITVK 787

BLAST of Tan0009395 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 3.9e-174
Identity = 340/756 (44.97%), Postives = 473/756 (62.57%), Query Frame = 0

Query: 11  SYIVYLG----THSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGF 70
           +YIV++       SF  + + YD+ L + S                  ++Y+Y   I+GF
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISD--------------SAELLYTYENAIHGF 90

Query: 71  AAVLDEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGED 130
           +  L ++EA +L   P V+SV      +LHTTR+  FLG+++     +  ++  A    D
Sbjct: 91  STRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----TADLFPEAGSYSD 150

Query: 131 TIIGNLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQ---CNKKLIGARYFYQGFAM 190
            ++G LDTG WPESKS++D G+GP+PS W+G CE G+NF    CN+KLIGAR+F +G+  
Sbjct: 151 VVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYES 210

Query: 191 THGPLNIS--LHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKV 250
           T GP++ S    + RD +GHG+HT STA G+ V G ++ G  +GTA+G +P+AR+A YKV
Sbjct: 211 TMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKV 270

Query: 251 CWPSNKDGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVV 310
           CW     GGCF SDILA  + AI+D V+VLS+SLG    ++  D ++IGAF A+++GI+V
Sbjct: 271 CWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILV 330

Query: 311 VCSAGNDGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKF 370
            CSAGN GPS  ++SNV+PW+ TVGA ++DRDF +   LGN K+F G SL        K 
Sbjct: 331 SCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKL 390

Query: 371 YPLINAVEAKAGNASD-TLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGV 430
            P I      AGNAS+ T   LC  G+L P K KGKI++C RG N RV KG  V   GGV
Sbjct: 391 LPFI-----YAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 450

Query: 431 GMILANDEKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPS 490
           GMILAN   +G  ++ D HLLPA+ V    G  I  Y+ +   P A ++ + T +G+KPS
Sbjct: 451 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 510

Query: 491 PVMASFSSRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSM 550
           PV+A+FSSRGPN IT +++KPD+ APGV+I+A+ T     T L  D+RRV FN+ SGTSM
Sbjct: 511 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 570

Query: 551 SCPHISGVVGLLKTLYPTWSPAAIKSAIMTTA-KTRDNTNMHSILLDSTKVGKGTPFDYG 610
           SCPH+SG+  LLK+++P WSPAAI+SA+MTTA KT  +      LLD       TPFD+G
Sbjct: 571 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKP---LLDIATGKPSTPFDHG 630

Query: 611 AGHVHPNNAMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVC--AEAFAITDLNY 670
           AGHV P  A +PGL+YD T +DYL FLCA  Y S  ++  S   + C  ++++++ DLNY
Sbjct: 631 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNY 690

Query: 671 PSISVPKLQIGAPFTINRRVKNVGISAGTYVARV-KMPKGVAVTVEPSTLQFNSVGEEKA 730
           PS +V    +GA +   R V +VG  AGTY  +V     GV ++VEP+ L F    E+K+
Sbjct: 691 PSFAVNVDGVGA-YKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKS 750

Query: 731 FKLVFQCNSKLQRQGYVFGTLVWSDGKHFVRSPIAV 753
           + + F  +S        FG++ WSDGKH V SP+A+
Sbjct: 751 YTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of Tan0009395 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 3.3e-165
Identity = 327/750 (43.60%), Postives = 466/750 (62.13%), Query Frame = 0

Query: 22  GSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVLDEKEATALAKN 81
           GS PSI+ T     S          ++ A +  I++ Y+   +GF+AV+   EA  L  +
Sbjct: 35  GSMPSIFPTHYHWYS----------TEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNH 94

Query: 82  PNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIGNLDTGAWPESK 141
           P V++VFE++ R+LHTTRS +FLG+++  G     +W  + +G D IIG  DTG WPE +
Sbjct: 95  PAVLAVFEDRRRELHTTRSPQFLGLQNQKG-----LWSESDYGSDVIIGVFDTGIWPERR 154

Query: 142 SFNDAGYGPVPSRWRGACEGGSNF---QCNKKLIGARYFYQG-FAMTHGPLN--ISLHTA 201
           SF+D   GP+P RWRG CE G+ F    CN+K+IGAR+F +G  A   G +N  +   + 
Sbjct: 155 SFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSP 214

Query: 202 RDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNKDGGCFDSD 261
           RD +GHG+HT STA G      ++ G  +G AKG +PKAR+AAYKVCW   KD GC DSD
Sbjct: 215 RDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCW---KDSGCLDSD 274

Query: 262 ILAGFEAAISDGVDVLSVSLGAA---AQEFAYDSLSIGAFHAIQQGIVVVCSAGNDGPSP 321
           ILA F+AA+ DGVDV+S+S+G        +  D ++IG++ A  +GI V  SAGN+GP+ 
Sbjct: 275 ILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNG 334

Query: 322 GTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLP-GGKFYPLINAVEAK 381
            +V+N++PW+ TVGAS+IDR+F +   LG+    +G SL + G+P  G+ +P++     K
Sbjct: 335 MSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA-GVPLNGRMFPVV--YPGK 394

Query: 382 AGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILANDEKDG 441
           +G +S   A LC E +LDP + +GKI++C RG + RV KG  V + GGVGMILAN   +G
Sbjct: 395 SGMSS---ASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNG 454

Query: 442 STIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFSSRGP 501
             ++ D HL+PA  V   +G  I  Y +S   P+A +    T +GIKP+PV+ASFS RGP
Sbjct: 455 EGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGP 514

Query: 502 NPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISGVVGL 561
           N ++  ++KPD+ APGV+I+A+ T+ V  T LP D R+  FN+ SGTSM+CPH+SG   L
Sbjct: 515 NGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAAL 574

Query: 562 LKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNNAMDP 621
           LK+ +P WSPA I+SA+MTT    DN+N    L+D +     TP+DYG+GH++   AM+P
Sbjct: 575 LKSAHPDWSPAVIRSAMMTTTNLVDNSNRS--LIDESTGKSATPYDYGSGHLNLGRAMNP 634

Query: 622 GLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAIT--DLNYPSISV--PKLQI 681
           GLVYD T DDY+ FLC+ GY    ++  +     C      +  +LNYPSI+   P  + 
Sbjct: 635 GLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRR 694

Query: 682 G-APFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSK 741
           G    T+ R   NVG +   Y AR++ P+GV VTV+P  L F S  + +++ +    N++
Sbjct: 695 GLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTR 754

Query: 742 ---LQRQGYVFGTLVWSD-GKHFVRSPIAV 753
              L   G VFG++ W D GKH VRSPI V
Sbjct: 755 NVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

BLAST of Tan0009395 vs. NCBI nr
Match: XP_038905686.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 606/745 (81.34%), Postives = 670/745 (89.93%), Query Frame = 0

Query: 11  SYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVL 70
           SYIVYLG HSFG NPS YD Q ATESQY+IL +V  SK+AAKESI+YSY+RYINGFAAVL
Sbjct: 27  SYIVYLGLHSFGLNPSKYDVQRATESQYDILSSVTGSKLAAKESIMYSYSRYINGFAAVL 86

Query: 71  DEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIG 130
           DEKEA ALAKNP+VVSVFENKERKLHTT+SW FLG++ D GIPSNSIWKAARFGEDTIIG
Sbjct: 87  DEKEAMALAKNPSVVSVFENKERKLHTTKSWSFLGVDSDAGIPSNSIWKAARFGEDTIIG 146

Query: 131 NLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGPLNI 190
           NLDTGAWPESKSFNDAGYGPVPSRWRGAC+GG+NF+CN+KLIGARYF QGFAM +GPLN+
Sbjct: 147 NLDTGAWPESKSFNDAGYGPVPSRWRGACDGGANFRCNRKLIGARYFNQGFAMANGPLNV 206

Query: 191 SLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNKDGG 250
           S +TARD EGHGSHTLSTAGGNFVPG N+FG GNGTAKGGSPKAR+AAYKVCWP+   GG
Sbjct: 207 SFNTARDKEGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSPKARVAAYKVCWPA-PTGG 266

Query: 251 CFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGNDGP 310
           CFDSDILAGFEAAI DGVDVLSVSLG  AQEFAYD++SIGAFHA+Q+GIVVVCS GNDGP
Sbjct: 267 CFDSDILAGFEAAIGDGVDVLSVSLGTGAQEFAYDAVSIGAFHAVQKGIVVVCSGGNDGP 326

Query: 311 SPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINAVEA 370
           SPGTVSNVSPWMFTV AS+IDRDF+SYV LGNKKH +GSSLSS GL G KFYPLINA+EA
Sbjct: 327 SPGTVSNVSPWMFTVAASTIDRDFASYVLLGNKKHIRGSSLSSSGLRGRKFYPLINAIEA 386

Query: 371 KAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILANDEKD 430
           KA NA+D+LAQ CE+GSLDP KAKGKIIVCLRG+N RV+K F VL  GGVGMI+ ND+KD
Sbjct: 387 KAANATDSLAQFCEQGSLDPAKAKGKIIVCLRGENARVEKSFVVLHAGGVGMIMVNDQKD 446

Query: 431 GSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFSSRG 490
           GS  + D H+LPA+HVSY DGLSI+QYI STKTPVA++THV+TE+GIKPSPVMA FSSRG
Sbjct: 447 GSGTLADAHILPATHVSYTDGLSISQYIKSTKTPVAYITHVKTEVGIKPSPVMADFSSRG 506

Query: 491 PNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISGVVG 550
           PN ITE+++KPDITAPGV+IIASVT D TATD PFDTRRVPFNVESGTSMSCPHISGV G
Sbjct: 507 PNSITEAMLKPDITAPGVNIIASVTIDATATDSPFDTRRVPFNVESGTSMSCPHISGVAG 566

Query: 551 LLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNNAMD 610
           LLKTLYPTWSPAAIKSAIMTTAKTRDNT     + D+ K+ K TPFDYGAGHVHPN+AMD
Sbjct: 567 LLKTLYPTWSPAAIKSAIMTTAKTRDNT--QRTISDTAKM-KATPFDYGAGHVHPNSAMD 626

Query: 611 PGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQIGAP 670
           PGLVYDTTIDDYLNFLCARGYNS+ALKKF N  F+CA++FAITDLNYPSISVP+L+IGAP
Sbjct: 627 PGLVYDTTIDDYLNFLCARGYNSVALKKFYNKPFICAKSFAITDLNYPSISVPELRIGAP 686

Query: 671 FTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSKLQRQ 730
            T+NRRVKNVG + GTYVARVK    ++V+VEPSTLQFNSVGEEKAFK+VFQ   K QRQ
Sbjct: 687 VTVNRRVKNVG-TPGTYVARVKASPAISVSVEPSTLQFNSVGEEKAFKVVFQYKGKEQRQ 746

Query: 731 GYVFGTLVWSDGKHFVRSPIAVKLG 756
           G+VFGTL+WSDGKHFVRSPIAVKLG
Sbjct: 747 GHVFGTLIWSDGKHFVRSPIAVKLG 766

BLAST of Tan0009395 vs. NCBI nr
Match: KAG6596782.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1214.1 bits (3140), Expect = 0.0e+00
Identity = 603/747 (80.72%), Postives = 664/747 (88.89%), Query Frame = 0

Query: 11  SYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVL 70
           SYIVYLG+HSFG NPSIYD QLATESQY+ILG+V  SK+AAK+SI+YSYNRYINGFAAVL
Sbjct: 25  SYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAVL 84

Query: 71  DEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIG 130
           DE+EATALAKNP+VVSVFENKERKLHTTRSW FLG++ D GIP NSIWKAARFGEDTIIG
Sbjct: 85  DEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDRGIPRNSIWKAARFGEDTIIG 144

Query: 131 NLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGPL-- 190
           NLDTG WPES SFNDAGYGPVPSRWRGACEGGS F+CN KLIGARYFY+GF    GPL  
Sbjct: 145 NLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNSKLIGARYFYRGFQAAEGPLRT 204

Query: 191 -NISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNK 250
            NIS  +ARDHEGHGSHTLSTAGGNFV GVNVFGNGNGTAKGGSPKAR+ AYKVCWPS K
Sbjct: 205 HNISFVSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPKARVVAYKVCWPS-K 264

Query: 251 DGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGN 310
           +GGC+DSDILAG EAAISDGVDVLS S+G  AQEFA D++SIGAFHA+Q GIVVV SAGN
Sbjct: 265 NGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHGIVVVSSAGN 324

Query: 311 DGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINA 370
           DGPSPG+VSNVSPWM TVGAS+IDRDF SYVALGNKK  +GSSLSS  LP GKFYPLI A
Sbjct: 325 DGPSPGSVSNVSPWMVTVGASTIDRDFVSYVALGNKKQLRGSSLSSSRLPAGKFYPLIKA 384

Query: 371 VEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILAND 430
           V+ KA NA+D  AQLC +G+LDPTKAKGKIIVCLRG+N RV KGFEVLRVGGVGM+L N+
Sbjct: 385 VQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVLRVGGVGMVLVNN 444

Query: 431 EKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFS 490
           + DGS ++ DPH+LPASH+SY DG+SIAQY++STKTPVA +TH  TE+GIKPSP+MASFS
Sbjct: 445 QTDGSALLADPHILPASHISYADGVSIAQYLSSTKTPVASITHASTEIGIKPSPLMASFS 504

Query: 491 SRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISG 550
           SRGP+ IT++VIKPDITAPGV+IIASVT+DVTA++LPFD RRV FNVESGTSMSCPHISG
Sbjct: 505 SRGPDFITQAVIKPDITAPGVNIIASVTKDVTASELPFDKRRVSFNVESGTSMSCPHISG 564

Query: 551 VVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNN 610
           V GLLKTL+PTWSPAAIKSAIMTTAKTRDNT   + +LD TKV K TPFDYGAGHVHPN+
Sbjct: 565 VAGLLKTLHPTWSPAAIKSAIMTTAKTRDNTK--NTMLDFTKV-KATPFDYGAGHVHPND 624

Query: 611 AMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQI 670
           AMDPGLVYDTT+DDYLNFLC +GYNS  LKKFSN  FVCA+ FAITDLNYPSISVPKLQI
Sbjct: 625 AMDPGLVYDTTVDDYLNFLCTQGYNSRTLKKFSNKPFVCAKKFAITDLNYPSISVPKLQI 684

Query: 671 GAPFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSKL 730
           GAP T+NRRVKNVG SAGTYVARV+MPKG+ V VEPS LQF++VGEEK FKLVF    KL
Sbjct: 685 GAPVTVNRRVKNVG-SAGTYVARVRMPKGITVMVEPSKLQFHNVGEEKPFKLVFHYAHKL 744

Query: 731 QRQGYVFGTLVWSDGKHFVRSPIAVKL 755
           +RQGYVFG LVWSDGKHFVRSPIAV L
Sbjct: 745 RRQGYVFGALVWSDGKHFVRSPIAVNL 766

BLAST of Tan0009395 vs. NCBI nr
Match: KAG6596779.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 595/747 (79.65%), Postives = 656/747 (87.82%), Query Frame = 0

Query: 11  SYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVL 70
           SYIVYLG+HSFG NPSIYD QLATESQY+ILG+V  SK+AAK+SI+YSYNRYINGFAAVL
Sbjct: 31  SYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAVL 90

Query: 71  DEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIG 130
           DE+EATALAKNP+VVSVFENKERKLHTTRSW FLG++ D GIP NSIWKAARFGEDTIIG
Sbjct: 91  DEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDRGIPRNSIWKAARFGEDTIIG 150

Query: 131 NLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGP--- 190
           NLDTG WPES SFNDAGYGPVPSRWRGACEGGS F+CN+KLIGARYFY+GF  +  P   
Sbjct: 151 NLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNRKLIGARYFYRGFQASERPWTK 210

Query: 191 LNISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNK 250
            NIS  +ARDHEGHGSHTLSTAGGNFV GVNVFGNGNGTAKGGS +AR+ AYKVCWPS +
Sbjct: 211 QNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSSRARVVAYKVCWPS-E 270

Query: 251 DGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGN 310
           +GGC+DSDILAG EAAISDGVDVLS SLG AAQEFA+D++SIGAFHA+Q GIVVVCSAGN
Sbjct: 271 NGGCYDSDILAGIEAAISDGVDVLSASLGMAAQEFAHDAISIGAFHAVQHGIVVVCSAGN 330

Query: 311 DGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINA 370
           DGPSPG+VSNVSPWM TVGAS+IDRDF SYV LGNKK F+GSSLSS  LP GKFYPLI A
Sbjct: 331 DGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKRFRGSSLSSSRLPAGKFYPLIKA 390

Query: 371 VEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILAND 430
           V+ KA NA+D  AQLC +G+LDPTKAKGKIIVCLRG+N RV KGFEV RVGG+GM+L N+
Sbjct: 391 VQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVHRVGGIGMVLVNN 450

Query: 431 EKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFS 490
           + DGS I+ DPH+LPASH+S  DG+SI QY++STKTPVA +TH  TE+GIKPSP+MASFS
Sbjct: 451 QIDGSAIVADPHILPASHLSDADGVSITQYLSSTKTPVASITHASTEMGIKPSPLMASFS 510

Query: 491 SRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISG 550
           SRGP+ ITE+VIKPDITAPGV+IIASVT D+TA+ LPFD RRVPFN+ESGTSMSCPHISG
Sbjct: 511 SRGPDFITEAVIKPDITAPGVNIIASVTNDITASGLPFDKRRVPFNIESGTSMSCPHISG 570

Query: 551 VVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNN 610
           V GLLKTL+PTWSP AIKSAIMTTAKTRDNT   + +LD TKV K TPFDYGAGHVHPNN
Sbjct: 571 VAGLLKTLHPTWSPGAIKSAIMTTAKTRDNTK--NTILDYTKV-KATPFDYGAGHVHPNN 630

Query: 611 AMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQI 670
           AMDPGLVYDTT+DDYLNFLC RGYNS  LKKFSN  FVCA+ FA TD NYPSI VP+LQI
Sbjct: 631 AMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCAKNFATTDFNYPSILVPRLQI 690

Query: 671 GAPFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSKL 730
           G   T+NRRVKNVG SAGTYVARVKMPKG+ V VEPSTLQF+SVGEEK FKLVF    KL
Sbjct: 691 GESVTVNRRVKNVG-SAGTYVARVKMPKGITVMVEPSTLQFHSVGEEKPFKLVFHYAHKL 750

Query: 731 QRQGYVFGTLVWSDGKHFVRSPIAVKL 755
           +RQGYVFG LVWSDGKHFVRS IAV L
Sbjct: 751 RRQGYVFGALVWSDGKHFVRSSIAVNL 772

BLAST of Tan0009395 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 587/745 (78.79%), Postives = 653/745 (87.65%), Query Frame = 0

Query: 11  SYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVL 70
           SYIVYLG  S GSNP+ YD ++ATESQY++LG+V+ SK+AAK++I YSYN+YINGFAA L
Sbjct: 55  SYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATL 114

Query: 71  DEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIG 130
           DEK+A  LAKNP VVSVFENKERKLHTTRSW FLG+E D GIPSNSIW A RFGEDTIIG
Sbjct: 115 DEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIG 174

Query: 131 NLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGPLNI 190
           NLDTG WPESKSFNDAGYGPVPSRWRGACEGG+NF+CN+KLIGARYF +GFAM  GPLNI
Sbjct: 175 NLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNI 234

Query: 191 SLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNKDGG 250
           S +TARD +GHGSHTLSTAGGNFVPG NVFG GNGTAKGGSPKAR+AAYKVCWP+   GG
Sbjct: 235 SFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGG 294

Query: 251 CFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGNDGP 310
           C+D+DILAGFEAAISDGVDVLSVSLG+  +EFAYDS+SIGAFHA+QQGIVVVCSAGNDGP
Sbjct: 295 CYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGP 354

Query: 311 SPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINAVEA 370
            PGTVSN+SPWMFTV ASSIDRDF+SY +LGNKKH+KGSS+SS  L GGKFYPLINAV+A
Sbjct: 355 GPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAVDA 414

Query: 371 KAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILANDEKD 430
           KA NAS+ LAQLC +GSLDPTKAKGKIIVCLRG+N RV+KGF VL+ GGVGMIL N +  
Sbjct: 415 KAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNG 474

Query: 431 GSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFSSRG 490
           GS    D H+LPA+H+SY DGL++AQYINSTKTPVA +T V+T+LGIKPSPVMA FSSRG
Sbjct: 475 GSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRG 534

Query: 491 PNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISGVVG 550
           PNPITE+++KPDIT PG+SI+ASVT DVTAT  PFDTRRVPFNVESGTSMSCPHISGVVG
Sbjct: 535 PNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVG 594

Query: 551 LLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNNAMD 610
           LLKTLYPTWSPAAIKSAIMTTAKTRDNT M +I  D+ K  K TPFDYGAGHVHPN+AMD
Sbjct: 595 LLKTLYPTWSPAAIKSAIMTTAKTRDNT-MRTI-SDNVK-PKATPFDYGAGHVHPNSAMD 654

Query: 611 PGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQIGAP 670
           PGLVYDTTIDDYLNFLCARGYNSL  K F N  FVCA++F +TDLNYPSIS+PKLQ GAP
Sbjct: 655 PGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAP 714

Query: 671 FTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSKLQRQ 730
            T+NRRVKNVG + GTYVARV     + VTVEPSTLQFNSVGEEKAFK+VF+     Q +
Sbjct: 715 VTVNRRVKNVG-TPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDK 774

Query: 731 GYVFGTLVWSDGKHFVRSPIAVKLG 756
           GYVFGTL+WSDGKH VRSPI V LG
Sbjct: 775 GYVFGTLIWSDGKHNVRSPIVVNLG 795

BLAST of Tan0009395 vs. NCBI nr
Match: XP_023005962.1 (uncharacterized protein LOC111498820 [Cucurbita maxima])

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 588/748 (78.61%), Postives = 655/748 (87.57%), Query Frame = 0

Query: 10   SSYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAV 69
            +SYIVYLG+HSF  NPS+YD QLATESQY+ILG+V  SK+AAK+SI+YSYNRYINGFAAV
Sbjct: 793  NSYIVYLGSHSFAPNPSVYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAV 852

Query: 70   LDEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTII 129
            LDE+EATALAKNP+VVSVFENKERKLHTTRSW FLG++ D GIP NSIWKAARFG DTII
Sbjct: 853  LDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDRGIPQNSIWKAARFGADTII 912

Query: 130  GNLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGPL- 189
            GNLDTG WPES SFNDAGYGPVPSRWRGACEGGS F+CN+KLIGARYFY+GF    GPL 
Sbjct: 913  GNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNRKLIGARYFYRGFQAAEGPLT 972

Query: 190  --NISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSN 249
              NIS  +ARDHEGHGSHTLSTAGGNFV GVNVFGNGNGTAKGGSP+AR+ AYKVCWPS 
Sbjct: 973  THNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPRARVVAYKVCWPS- 1032

Query: 250  KDGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAG 309
            K+GGC+DSDILAG EAAISDGVDVLS S+G  AQEFA D++SIGAFHA+Q GIVVVCSAG
Sbjct: 1033 KNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHGIVVVCSAG 1092

Query: 310  NDGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLIN 369
            NDGPSPG+VSNVSPWM TVGAS+IDRDF SYV LGNKK F+GSSLSS  LP GKFYPLI 
Sbjct: 1093 NDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKRFRGSSLSSSRLPAGKFYPLIK 1152

Query: 370  AVEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILAN 429
            AV+ KA NA+D  AQLC +G+LDPTKAKGKIIVCLRG+N RV KGFEV RVGGVGM+L N
Sbjct: 1153 AVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVFRVGGVGMVLVN 1212

Query: 430  DEKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASF 489
            ++ DGS ++ DPH+LPASH+SY DG+SIAQY++STKTPVA +TH  TE+GIKPSP+MASF
Sbjct: 1213 NQMDGSALVADPHILPASHLSYADGVSIAQYLSSTKTPVASITHASTEMGIKPSPLMASF 1272

Query: 490  SSRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHIS 549
            SSRGP+ IT++VIKPDITAPGV+IIASVT+D++A+ LP D RRVPFN+ESGTSMSCPHIS
Sbjct: 1273 SSRGPDFITQAVIKPDITAPGVNIIASVTDDISASGLPLDKRRVPFNIESGTSMSCPHIS 1332

Query: 550  GVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPN 609
            GV GLLKTL+PTWSPAAIKSA+MTTAKTRDNT   + LLD TKV K TPFDYGAGHVHPN
Sbjct: 1333 GVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTK--NTLLDYTKV-KATPFDYGAGHVHPN 1392

Query: 610  NAMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQ 669
            +AMDPGLVYDTT+DDYLNFLC RGYNS  LKKFSN  FVCA  FA TD NYPSI VP+LQ
Sbjct: 1393 DAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCANNFATTDFNYPSILVPRLQ 1452

Query: 670  IGAPFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSK 729
            IG   T+NRRVKNVG S GTYVARV+MPKG+ V VEPS LQF+SVGEE+ FKL+F    K
Sbjct: 1453 IGGSVTVNRRVKNVG-STGTYVARVRMPKGITVMVEPSKLQFHSVGEERPFKLIFHYAHK 1512

Query: 730  LQRQGYVFGTLVWSDGKHFVRSPIAVKL 755
            L+RQGYVFG LVWSDGKHFVRS IAV L
Sbjct: 1513 LRRQGYVFGALVWSDGKHFVRSSIAVNL 1535

BLAST of Tan0009395 vs. ExPASy TrEMBL
Match: A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 587/745 (78.79%), Postives = 653/745 (87.65%), Query Frame = 0

Query: 11  SYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVL 70
           SYIVYLG  S GSNP+ YD ++ATESQY++LG+V+ SK+AAK++I YSYN+YINGFAA L
Sbjct: 6   SYIVYLGAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATL 65

Query: 71  DEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIG 130
           DEK+A  LAKNP VVSVFENKERKLHTTRSW FLG+E D GIPSNSIW A RFGEDTIIG
Sbjct: 66  DEKQAKDLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIG 125

Query: 131 NLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGPLNI 190
           NLDTG WPESKSFNDAGYGPVPSRWRGACEGG+NF+CN+KLIGARYF +GFAM  GPLNI
Sbjct: 126 NLDTGVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNI 185

Query: 191 SLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNKDGG 250
           S +TARD +GHGSHTLSTAGGNFVPG NVFG GNGTAKGGSPKAR+AAYKVCWP+   GG
Sbjct: 186 SFNTARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGG 245

Query: 251 CFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGNDGP 310
           C+D+DILAGFEAAISDGVDVLSVSLG+  +EFAYDS+SIGAFHA+QQGIVVVCSAGNDGP
Sbjct: 246 CYDADILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGP 305

Query: 311 SPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINAVEA 370
            PGTVSN+SPWMFTV ASSIDRDF+SY +LGNKKH+KGSS+SS  L GGKFYPLINAV+A
Sbjct: 306 GPGTVSNISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAVDA 365

Query: 371 KAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILANDEKD 430
           KA NAS+ LAQLC +GSLDPTKAKGKIIVCLRG+N RV+KGF VL+ GGVGMIL N +  
Sbjct: 366 KAANASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNG 425

Query: 431 GSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFSSRG 490
           GS    D H+LPA+H+SY DGL++AQYINSTKTPVA +T V+T+LGIKPSPVMA FSSRG
Sbjct: 426 GSGTTADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRG 485

Query: 491 PNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISGVVG 550
           PNPITE+++KPDIT PG+SI+ASVT DVTAT  PFDTRRVPFNVESGTSMSCPHISGVVG
Sbjct: 486 PNPITEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVG 545

Query: 551 LLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNNAMD 610
           LLKTLYPTWSPAAIKSAIMTTAKTRDNT M +I  D+ K  K TPFDYGAGHVHPN+AMD
Sbjct: 546 LLKTLYPTWSPAAIKSAIMTTAKTRDNT-MRTI-SDNVK-PKATPFDYGAGHVHPNSAMD 605

Query: 611 PGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQIGAP 670
           PGLVYDTTIDDYLNFLCARGYNSL  K F N  FVCA++F +TDLNYPSIS+PKLQ GAP
Sbjct: 606 PGLVYDTTIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAP 665

Query: 671 FTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSKLQRQ 730
            T+NRRVKNVG + GTYVARV     + VTVEPSTLQFNSVGEEKAFK+VF+     Q +
Sbjct: 666 VTVNRRVKNVG-TPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDK 725

Query: 731 GYVFGTLVWSDGKHFVRSPIAVKLG 756
           GYVFGTL+WSDGKH VRSPI V LG
Sbjct: 726 GYVFGTLIWSDGKHNVRSPIVVNLG 746

BLAST of Tan0009395 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 588/748 (78.61%), Postives = 655/748 (87.57%), Query Frame = 0

Query: 10   SSYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAV 69
            +SYIVYLG+HSF  NPS+YD QLATESQY+ILG+V  SK+AAK+SI+YSYNRYINGFAAV
Sbjct: 793  NSYIVYLGSHSFAPNPSVYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAV 852

Query: 70   LDEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTII 129
            LDE+EATALAKNP+VVSVFENKERKLHTTRSW FLG++ D GIP NSIWKAARFG DTII
Sbjct: 853  LDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDRGIPQNSIWKAARFGADTII 912

Query: 130  GNLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGPL- 189
            GNLDTG WPES SFNDAGYGPVPSRWRGACEGGS F+CN+KLIGARYFY+GF    GPL 
Sbjct: 913  GNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNRKLIGARYFYRGFQAAEGPLT 972

Query: 190  --NISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSN 249
              NIS  +ARDHEGHGSHTLSTAGGNFV GVNVFGNGNGTAKGGSP+AR+ AYKVCWPS 
Sbjct: 973  THNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPRARVVAYKVCWPS- 1032

Query: 250  KDGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAG 309
            K+GGC+DSDILAG EAAISDGVDVLS S+G  AQEFA D++SIGAFHA+Q GIVVVCSAG
Sbjct: 1033 KNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHGIVVVCSAG 1092

Query: 310  NDGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLIN 369
            NDGPSPG+VSNVSPWM TVGAS+IDRDF SYV LGNKK F+GSSLSS  LP GKFYPLI 
Sbjct: 1093 NDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKRFRGSSLSSSRLPAGKFYPLIK 1152

Query: 370  AVEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILAN 429
            AV+ KA NA+D  AQLC +G+LDPTKAKGKIIVCLRG+N RV KGFEV RVGGVGM+L N
Sbjct: 1153 AVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVFRVGGVGMVLVN 1212

Query: 430  DEKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASF 489
            ++ DGS ++ DPH+LPASH+SY DG+SIAQY++STKTPVA +TH  TE+GIKPSP+MASF
Sbjct: 1213 NQMDGSALVADPHILPASHLSYADGVSIAQYLSSTKTPVASITHASTEMGIKPSPLMASF 1272

Query: 490  SSRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHIS 549
            SSRGP+ IT++VIKPDITAPGV+IIASVT+D++A+ LP D RRVPFN+ESGTSMSCPHIS
Sbjct: 1273 SSRGPDFITQAVIKPDITAPGVNIIASVTDDISASGLPLDKRRVPFNIESGTSMSCPHIS 1332

Query: 550  GVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPN 609
            GV GLLKTL+PTWSPAAIKSA+MTTAKTRDNT   + LLD TKV K TPFDYGAGHVHPN
Sbjct: 1333 GVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTK--NTLLDYTKV-KATPFDYGAGHVHPN 1392

Query: 610  NAMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQ 669
            +AMDPGLVYDTT+DDYLNFLC RGYNS  LKKFSN  FVCA  FA TD NYPSI VP+LQ
Sbjct: 1393 DAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCANNFATTDFNYPSILVPRLQ 1452

Query: 670  IGAPFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSK 729
            IG   T+NRRVKNVG S GTYVARV+MPKG+ V VEPS LQF+SVGEE+ FKL+F    K
Sbjct: 1453 IGGSVTVNRRVKNVG-STGTYVARVRMPKGITVMVEPSKLQFHSVGEERPFKLIFHYAHK 1512

Query: 730  LQRQGYVFGTLVWSDGKHFVRSPIAVKL 755
            L+RQGYVFG LVWSDGKHFVRS IAV L
Sbjct: 1513 LRRQGYVFGALVWSDGKHFVRSSIAVNL 1535

BLAST of Tan0009395 vs. ExPASy TrEMBL
Match: A0A6J1GDW2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453052 PE=3 SV=1)

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 591/747 (79.12%), Postives = 657/747 (87.95%), Query Frame = 0

Query: 11  SYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVL 70
           SYIVYLG+HSFG NPSIYD QLATESQY+ILG+V  SK+AAK+SI+YSYNRYINGFAAVL
Sbjct: 31  SYIVYLGSHSFGPNPSIYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAVL 90

Query: 71  DEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIG 130
           D++EATALAKNP+VVS+FENKERKLHTTRSW FLG++ D GIP NSIWKAARFGEDTIIG
Sbjct: 91  DDQEATALAKNPSVVSIFENKERKLHTTRSWSFLGVDSDRGIPRNSIWKAARFGEDTIIG 150

Query: 131 NLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGP--- 190
           NLDTG WPES SFNDAGYGPVP+RWRGAC+GGS F+CN+KLIGARYFY+GF  +  P   
Sbjct: 151 NLDTGVWPESPSFNDAGYGPVPARWRGACDGGSKFRCNRKLIGARYFYRGFQASERPWTK 210

Query: 191 LNISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNK 250
            NIS  +A+DHEGHGSHTLSTAGGNFV GVNVFGNGNGTAKGGSP+AR+ AYKVCWPS +
Sbjct: 211 QNISFDSAQDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPRARVVAYKVCWPS-E 270

Query: 251 DGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGN 310
           +GGC+DSDILAG EAAISDGVDVLS SLG AAQEFA+D++SIGAFHA+Q GIVVVCSAGN
Sbjct: 271 NGGCYDSDILAGIEAAISDGVDVLSASLGMAAQEFAHDAISIGAFHAVQHGIVVVCSAGN 330

Query: 311 DGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINA 370
           DGPSPG+VSNVSPWM TVGAS+IDRDF SYVALGNKK  +GSSLSS  LP GKFYPLI A
Sbjct: 331 DGPSPGSVSNVSPWMVTVGASTIDRDFVSYVALGNKKQLRGSSLSSSRLPAGKFYPLIKA 390

Query: 371 VEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILAND 430
           V+ KA NA+D  AQLC +G+LDPTKAKGKIIVCLRG+N RV KGFEV RVGG+GM+L N+
Sbjct: 391 VQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVHRVGGIGMVLVNN 450

Query: 431 EKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFS 490
           + DGS I+ DPH+LPASH+S  DG+SI QY++STKTPVA +TH  TE+GIKPSP+MASFS
Sbjct: 451 QIDGSAIVADPHILPASHLSDADGVSITQYLSSTKTPVASITHASTEMGIKPSPLMASFS 510

Query: 491 SRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISG 550
           SRGP+ ITE+VIKPDITAPGV+IIASVT D+TA+ LPFD RRVPFN+ESGTSMSCPHISG
Sbjct: 511 SRGPDFITEAVIKPDITAPGVNIIASVTNDITASGLPFDKRRVPFNIESGTSMSCPHISG 570

Query: 551 VVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNN 610
           V GLLKTL+PTWSP AIKSAIMTTAKTRDNT   + +LD TKV K TPFDYGAGHVHPNN
Sbjct: 571 VAGLLKTLHPTWSPGAIKSAIMTTAKTRDNTK--NTILDYTKV-KATPFDYGAGHVHPNN 630

Query: 611 AMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQI 670
           AMDPGLVYDTT+DDYLNFLC RGYNS  LKKFSN  FVCA+ FA TD NYPSI VP+LQI
Sbjct: 631 AMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCAKNFATTDFNYPSILVPRLQI 690

Query: 671 GAPFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSKL 730
           G   T+NRRVKNVG SAGTYVARVKMPKG+ V VEPSTLQF+SVGEEK FKLVF    KL
Sbjct: 691 GESVTVNRRVKNVG-SAGTYVARVKMPKGITVMVEPSTLQFHSVGEEKPFKLVFHYAHKL 750

Query: 731 QRQGYVFGTLVWSDGKHFVRSPIAVKL 755
           +RQGYVFG LVWSDGKHFVRS IAV L
Sbjct: 751 RRQGYVFGALVWSDGKHFVRSSIAVNL 772

BLAST of Tan0009395 vs. ExPASy TrEMBL
Match: A0A6J1KYS5 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498344 PE=3 SV=1)

HSP 1 Score: 1189.9 bits (3077), Expect = 0.0e+00
Identity = 588/749 (78.50%), Postives = 655/749 (87.45%), Query Frame = 0

Query: 10  SSYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAV 69
           +SYIVYLG+HSF  NPS+YD QLATESQY+ILG+V  SK+AAK+SI+YSYNRYINGFAAV
Sbjct: 26  NSYIVYLGSHSFAPNPSVYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAV 85

Query: 70  LDEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTII 129
           LDE+EATALAKNP+VVSVFENKERKLHTTRSW FLG++ D GIP NSIWKAARFG DTII
Sbjct: 86  LDEQEATALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDRGIPQNSIWKAARFGADTII 145

Query: 130 GNLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGPL- 189
           GNLDTG WPES SFNDAGYGPVPSRWRGACEGGS F+CN+KLIGARYFY+GF    GPL 
Sbjct: 146 GNLDTGVWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNRKLIGARYFYRGFQAAEGPLT 205

Query: 190 --NISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSN 249
             NIS  +ARDHEGHGSHTLSTAGGNFV GVNVFGNGNGTAKGGSP+AR+ AYKVCWPS 
Sbjct: 206 THNISFDSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPRARVVAYKVCWPS- 265

Query: 250 KDGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAG 309
           K+GGC+DSDILAG EAAISDGVDVLS S+G  AQEFA D++SIGAFHA+Q GIVVVCSAG
Sbjct: 266 KNGGCYDSDILAGIEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHGIVVVCSAG 325

Query: 310 NDGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLIN 369
           NDGPSPG+VSNVSPWM TVGAS+IDRDF SYV LGNKK F+GSSLSS  LP GKFYPLI 
Sbjct: 326 NDGPSPGSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKRFRGSSLSSSRLPAGKFYPLIK 385

Query: 370 AVEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILAN 429
           AV+ KA NA+D  AQLC +G+LDPTKAKGKIIVCLRG+N RV KGFEV RVGGVGM+L N
Sbjct: 386 AVQVKAANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVFRVGGVGMVLVN 445

Query: 430 DEKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASF 489
           ++ DGS ++ DPH+LPASH+SY DG+SIAQY++STKTPVA +TH  TE+GIKPSP+MASF
Sbjct: 446 NQMDGSALVADPHILPASHLSYADGVSIAQYLSSTKTPVASITHASTEMGIKPSPLMASF 505

Query: 490 SSRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHIS 549
           SSRGP+ IT++VIKPDITAPGV+IIASVT+D++A+ LP D RRVPFN+ESGTSMSCPHIS
Sbjct: 506 SSRGPDFITQAVIKPDITAPGVNIIASVTDDISASGLPLDKRRVPFNIESGTSMSCPHIS 565

Query: 550 GVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPN 609
           GV GLLKTL+PTWSPAAIKSA+MTTAKTRDNT   + LLD TKV K TPFDYGAGHVHPN
Sbjct: 566 GVAGLLKTLHPTWSPAAIKSAVMTTAKTRDNTK--NTLLDYTKV-KATPFDYGAGHVHPN 625

Query: 610 NAMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQ 669
           +AMDPGLVYDTT+DDYLNFLC RGYNS  LKKFSN  FVCA  FA TD NYPSI VP+LQ
Sbjct: 626 DAMDPGLVYDTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCANNFATTDFNYPSILVPRLQ 685

Query: 670 IGAPFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSK 729
           IG   T+NRRVKNVG S GTYVARV+MPKG+ V VEPS LQF+SVGEE+ FKL+F    K
Sbjct: 686 IGGSVTVNRRVKNVG-STGTYVARVRMPKGITVMVEPSKLQFHSVGEERPFKLIFHYAHK 745

Query: 730 LQRQ-GYVFGTLVWSDGKHFVRSPIAVKL 755
           L+RQ GYVFG LVWSDGKHFVRS IAV L
Sbjct: 746 LRRQGGYVFGALVWSDGKHFVRSSIAVNL 769

BLAST of Tan0009395 vs. ExPASy TrEMBL
Match: A0A6J1GDR2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE=3 SV=1)

HSP 1 Score: 1184.5 bits (3063), Expect = 0.0e+00
Identity = 592/746 (79.36%), Postives = 650/746 (87.13%), Query Frame = 0

Query: 11  SYIVYLGTHS--FGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAA 70
           SYIVYLG+ S  FGSNPSIYD QLATES+Y+ILGTV  SKMAAKESI+Y+YNR INGFAA
Sbjct: 27  SYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGSKMAAKESILYTYNRCINGFAA 86

Query: 71  VLDEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTI 130
           VLD+ E TALAKNP+VVSVFEN+ERKLHTTRSW FLG++   GIP NSIWKA++FGED I
Sbjct: 87  VLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDSGRGIPQNSIWKASKFGEDVI 146

Query: 131 IGNLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGFAMTHGPL 190
           IGNLDTG WPES+SF+DAGYGPVPSRW GACEGGS F+CN+KLIGARYFY+G+ M +GPL
Sbjct: 147 IGNLDTGVWPESESFHDAGYGPVPSRWMGACEGGSKFRCNRKLIGARYFYRGYEMINGPL 206

Query: 191 NISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNKD 250
           NIS   ARDHEGHG+HTLSTAGGNFV G NVFGNGNGTAKGG+PKAR+AAYKVCWP  K 
Sbjct: 207 NISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNGTAKGGAPKARVAAYKVCWPQGK- 266

Query: 251 GGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGND 310
             C D+D+LAG EAAISDGVDVLS+SLGA AQ+FA D +S+GAFHAIQQGI+VVCSAGND
Sbjct: 267 --CSDADLLAGIEAAISDGVDVLSISLGATAQDFANDPISVGAFHAIQQGIIVVCSAGND 326

Query: 311 GPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINAV 370
           GP PGTV+NVSPWMFTVGASSIDR F SYV LGNKK  KGSSLSS GLP GK YPL+N+V
Sbjct: 327 GPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQIKGSSLSSGGLPRGKLYPLMNSV 386

Query: 371 EAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILANDE 430
            AKA NASD LAQLCEEGSLDP KA+GKIIVCLRGDNGR+DK FEVLRVGGVGMIL ND+
Sbjct: 387 NAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDK 446

Query: 431 KDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFSS 490
             GS I  DPH+LP SHVSY+DGLSIAQY+ STK PVA +T VRTE+GIKPSPVMA+FSS
Sbjct: 447 ISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVATITPVRTEIGIKPSPVMATFSS 506

Query: 491 RGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISGV 550
           RGPN ITE++IKPDITAPGV+IIAS T    ATDLPFD RRVPFNV+SGTSMSCPHISGV
Sbjct: 507 RGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFDKRRVPFNVQSGTSMSCPHISGV 566

Query: 551 VGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNNA 610
            GLLK L+PTWSPAAIKSAIMTTAKTRDNT   + +LD  KV K TPFDYGAG VHPNNA
Sbjct: 567 AGLLKKLHPTWSPAAIKSAIMTTAKTRDNTK--NTMLDFNKV-KATPFDYGAGLVHPNNA 626

Query: 611 MDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAITDLNYPSISVPKLQIG 670
           MDPGLVYDTTIDDYLNFLC +GYNSL LKKFSN  FVC++ FAITDLNYPSISVPKLQIG
Sbjct: 627 MDPGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCSKNFAITDLNYPSISVPKLQIG 686

Query: 671 APFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSKLQ 730
           AP T+NRRVKNVG SAGTYVARV+MPKG+ V VEPSTLQF+ VGEEK FKLVF    +++
Sbjct: 687 APVTVNRRVKNVG-SAGTYVARVRMPKGITVMVEPSTLQFHGVGEEKPFKLVFHYAQQVR 746

Query: 731 RQGYVFGTLVWSDGKHFVRSPIAVKL 755
           R GYVFG LVWSDGKHFVRSPIAV L
Sbjct: 747 RPGYVFGALVWSDGKHFVRSPIAVNL 765

BLAST of Tan0009395 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 840.5 bits (2170), Expect = 1.1e-243
Identity = 423/748 (56.55%), Postives = 541/748 (72.33%), Query Frame = 0

Query: 10  SSYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAV 69
           SSY+VY G HS     +        E+ Y+ LG+   S+  A ++I YSY ++INGFAA 
Sbjct: 30  SSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAH 89

Query: 70  LDEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTII 129
           LD   A  ++K+P VVSVF NK  KLHTTRSW FLG+E +  +PS+SIW+ ARFGEDTII
Sbjct: 90  LDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTII 149

Query: 130 GNLDTGAWPESKSFNDAGYGPVPSRWRGACEG--GSNFQCNKKLIGARYFYQGFAMTHGP 189
            NLDTG WPESKSF D G GP+PSRW+G C+    + F CN+KLIGARYF +G+A   G 
Sbjct: 150 ANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGH 209

Query: 190 LNISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNK 249
           LN S  + RD +GHGSHTLSTA G+FVPGV++FG GNGTAKGGSP+AR+AAYKVCWP  K
Sbjct: 210 LNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVK 269

Query: 250 DGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGN 309
              C+D+D+LA F+AAI DG DV+SVSLG     F  DS++IG+FHA ++ IVVVCSAGN
Sbjct: 270 GNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGN 329

Query: 310 DGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINA 369
            GP+  TVSNV+PW  TVGAS++DR+F+S + LGN KH+KG SLSS  LP  KFYP++ +
Sbjct: 330 SGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMAS 389

Query: 370 VEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILAND 429
           V AKA NAS   AQLC+ GSLDP K KGKI+VCLRG NGRV+KG  V   GG+GM+L N 
Sbjct: 390 VNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENT 449

Query: 430 EKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFS 489
              G+ ++ DPH+LPA+ ++  D  ++++YI+ TK P+A +T  RT+LG+KP+PVMASFS
Sbjct: 450 YVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFS 509

Query: 490 SRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISG 549
           S+GP+ +   ++KPDITAPGVS+IA+ T  V+ T+  FD RR+ FN  SGTSMSCPHISG
Sbjct: 510 SKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISG 569

Query: 550 VVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNN 609
           + GLLKT YP+WSPAAI+SAIMTTA   D  ++   + ++T + K TPF +GAGHV PN 
Sbjct: 570 IAGLLKTRYPSWSPAAIRSAIMTTATIMD--DIPGPIQNATNM-KATPFSFGAGHVQPNL 629

Query: 610 AMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAE-AFAITDLNYPSISVPKLQ 669
           A++PGLVYD  I DYLNFLC+ GYN+  +  FS N F C+    ++ +LNYPSI+VP L 
Sbjct: 630 AVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNL- 689

Query: 670 IGAPFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSK 729
             +  T++R VKNVG     Y  +V  P+GV V V+P++L F  VGE+K FK++   +  
Sbjct: 690 TSSKVTVSRTVKNVG-RPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKG 749

Query: 730 LQRQGYVFGTLVWSDGKHFVRSPIAVKL 755
              +GYVFG LVWSD KH VRSPI VKL
Sbjct: 750 NVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Tan0009395 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 817.8 bits (2111), Expect = 7.4e-237
Identity = 420/746 (56.30%), Postives = 517/746 (69.30%), Query Frame = 0

Query: 11  SYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVL 70
           SYIVYLG+H+     S         S    L + + S   AKE+I YSY R+INGFAA+L
Sbjct: 41  SYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAIL 100

Query: 71  DEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIG 130
           DE EA  +AK+P+VVSVF NK RKLHTT SW F+ +  +G +  +S+W  A +GEDTII 
Sbjct: 101 DENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIA 160

Query: 131 NLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQCNKKLIGARYFYQGF-AMTHGPLN 190
           NLDTG WPESKSF+D GYG VP+RW+G C    +  CN+KLIGARYF +G+ A T  P N
Sbjct: 161 NLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPSN 220

Query: 191 ISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNKDG 250
            S  T RDH+GHGSHTLSTA GNFVPG NVFG GNGTA GGSPKAR+AAYKVCWP     
Sbjct: 221 ASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGA 280

Query: 251 GCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVVCSAGNDG 310
            CFD+DILA  EAAI DGVDVLS S+G  A ++  D ++IG+FHA++ G+ VVCSAGN G
Sbjct: 281 ECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSG 340

Query: 311 PSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKFYPLINAVE 370
           P  GTVSNV+PW+ TVGASS+DR+F ++V L N + FKG+SL SK LP  K Y LI+A +
Sbjct: 341 PKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSL-SKPLPEEKMYSLISAAD 400

Query: 371 AKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILANDEK 430
           A   N + T A LC++GSLDP K KGKI+VCLRGDN RVDKG +    G  GM+L ND+ 
Sbjct: 401 ANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKA 460

Query: 431 DGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFSSR 490
            G+ I+ D H+LPAS + Y DG ++  Y++STK P  ++      L  KP+P MASFSSR
Sbjct: 461 SGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSR 520

Query: 491 GPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISGVV 550
           GPN IT  ++KPDITAPGV+IIA+ TE    TDL  D RR PFN ESGTSMSCPHISGVV
Sbjct: 521 GPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVV 580

Query: 551 GLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNNAM 610
           GLLKTL+P WSPAAI+SAIMTT++TR+N     +        K  PF YG+GHV PN A 
Sbjct: 581 GLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMV---DESFKKANPFSYGSGHVQPNKAA 640

Query: 611 DPGLVYDTTIDDYLNFLCARGYNSLALKKFSNN-QFVCAEAFAITDLNYPSISVPKLQIG 670
            PGLVYD T  DYL+FLCA GYN+  ++ F+ + Q+ C +   + D NYPSI+VP L   
Sbjct: 641 HPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLT-- 700

Query: 671 APFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSKLQ 730
              T+ R++KNVG  A TY AR + P GV V+VEP  L FN  GE K F++  +    + 
Sbjct: 701 GSITVTRKLKNVGPPA-TYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLR-PLPVT 760

Query: 731 RQGYVFGTLVWSDGKHFVRSPIAVKL 755
             GYVFG L W+D  H+VRSPI V+L
Sbjct: 761 PSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Tan0009395 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 613.2 bits (1580), Expect = 2.8e-175
Identity = 340/756 (44.97%), Postives = 473/756 (62.57%), Query Frame = 0

Query: 11  SYIVYLG----THSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGF 70
           +YIV++       SF  + + YD+ L + S                  ++Y+Y   I+GF
Sbjct: 31  TYIVHMAKSQMPSSFDLHSNWYDSSLRSISD--------------SAELLYTYENAIHGF 90

Query: 71  AAVLDEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGED 130
           +  L ++EA +L   P V+SV      +LHTTR+  FLG+++     +  ++  A    D
Sbjct: 91  STRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----TADLFPEAGSYSD 150

Query: 131 TIIGNLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNFQ---CNKKLIGARYFYQGFAM 190
            ++G LDTG WPESKS++D G+GP+PS W+G CE G+NF    CN+KLIGAR+F +G+  
Sbjct: 151 VVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYES 210

Query: 191 THGPLNIS--LHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKV 250
           T GP++ S    + RD +GHG+HT STA G+ V G ++ G  +GTA+G +P+AR+A YKV
Sbjct: 211 TMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKV 270

Query: 251 CWPSNKDGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVV 310
           CW     GGCF SDILA  + AI+D V+VLS+SLG    ++  D ++IGAF A+++GI+V
Sbjct: 271 CWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILV 330

Query: 311 VCSAGNDGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLPGGKF 370
            CSAGN GPS  ++SNV+PW+ TVGA ++DRDF +   LGN K+F G SL        K 
Sbjct: 331 SCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKL 390

Query: 371 YPLINAVEAKAGNASD-TLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGV 430
            P I      AGNAS+ T   LC  G+L P K KGKI++C RG N RV KG  V   GGV
Sbjct: 391 LPFI-----YAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 450

Query: 431 GMILANDEKDGSTIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPS 490
           GMILAN   +G  ++ D HLLPA+ V    G  I  Y+ +   P A ++ + T +G+KPS
Sbjct: 451 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 510

Query: 491 PVMASFSSRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSM 550
           PV+A+FSSRGPN IT +++KPD+ APGV+I+A+ T     T L  D+RRV FN+ SGTSM
Sbjct: 511 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 570

Query: 551 SCPHISGVVGLLKTLYPTWSPAAIKSAIMTTA-KTRDNTNMHSILLDSTKVGKGTPFDYG 610
           SCPH+SG+  LLK+++P WSPAAI+SA+MTTA KT  +      LLD       TPFD+G
Sbjct: 571 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKP---LLDIATGKPSTPFDHG 630

Query: 611 AGHVHPNNAMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVC--AEAFAITDLNY 670
           AGHV P  A +PGL+YD T +DYL FLCA  Y S  ++  S   + C  ++++++ DLNY
Sbjct: 631 AGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNY 690

Query: 671 PSISVPKLQIGAPFTINRRVKNVGISAGTYVARV-KMPKGVAVTVEPSTLQFNSVGEEKA 730
           PS +V    +GA +   R V +VG  AGTY  +V     GV ++VEP+ L F    E+K+
Sbjct: 691 PSFAVNVDGVGA-YKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKS 750

Query: 731 FKLVFQCNSKLQRQGYVFGTLVWSDGKHFVRSPIAV 753
           + + F  +S        FG++ WSDGKH V SP+A+
Sbjct: 751 YTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of Tan0009395 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 583.6 bits (1503), Expect = 2.3e-166
Identity = 327/750 (43.60%), Postives = 466/750 (62.13%), Query Frame = 0

Query: 22  GSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAVLDEKEATALAKN 81
           GS PSI+ T     S          ++ A +  I++ Y+   +GF+AV+   EA  L  +
Sbjct: 35  GSMPSIFPTHYHWYS----------TEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNH 94

Query: 82  PNVVSVFENKERKLHTTRSWRFLGMEDDGGIPSNSIWKAARFGEDTIIGNLDTGAWPESK 141
           P V++VFE++ R+LHTTRS +FLG+++  G     +W  + +G D IIG  DTG WPE +
Sbjct: 95  PAVLAVFEDRRRELHTTRSPQFLGLQNQKG-----LWSESDYGSDVIIGVFDTGIWPERR 154

Query: 142 SFNDAGYGPVPSRWRGACEGGSNF---QCNKKLIGARYFYQG-FAMTHGPLN--ISLHTA 201
           SF+D   GP+P RWRG CE G+ F    CN+K+IGAR+F +G  A   G +N  +   + 
Sbjct: 155 SFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSP 214

Query: 202 RDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVCWPSNKDGGCFDSD 261
           RD +GHG+HT STA G      ++ G  +G AKG +PKAR+AAYKVCW   KD GC DSD
Sbjct: 215 RDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCW---KDSGCLDSD 274

Query: 262 ILAGFEAAISDGVDVLSVSLGAA---AQEFAYDSLSIGAFHAIQQGIVVVCSAGNDGPSP 321
           ILA F+AA+ DGVDV+S+S+G        +  D ++IG++ A  +GI V  SAGN+GP+ 
Sbjct: 275 ILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNG 334

Query: 322 GTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSLSSKGLP-GGKFYPLINAVEAK 381
            +V+N++PW+ TVGAS+IDR+F +   LG+    +G SL + G+P  G+ +P++     K
Sbjct: 335 MSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA-GVPLNGRMFPVV--YPGK 394

Query: 382 AGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVGMILANDEKDG 441
           +G +S   A LC E +LDP + +GKI++C RG + RV KG  V + GGVGMILAN   +G
Sbjct: 395 SGMSS---ASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNG 454

Query: 442 STIMVDPHLLPASHVSYVDGLSIAQYINSTKTPVAFLTHVRTELGIKPSPVMASFSSRGP 501
             ++ D HL+PA  V   +G  I  Y +S   P+A +    T +GIKP+PV+ASFS RGP
Sbjct: 455 EGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGP 514

Query: 502 NPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVESGTSMSCPHISGVVGL 561
           N ++  ++KPD+ APGV+I+A+ T+ V  T LP D R+  FN+ SGTSM+CPH+SG   L
Sbjct: 515 NGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAAL 574

Query: 562 LKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTPFDYGAGHVHPNNAMDP 621
           LK+ +P WSPA I+SA+MTT    DN+N    L+D +     TP+DYG+GH++   AM+P
Sbjct: 575 LKSAHPDWSPAVIRSAMMTTTNLVDNSNRS--LIDESTGKSATPYDYGSGHLNLGRAMNP 634

Query: 622 GLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVCAEAFAIT--DLNYPSISV--PKLQI 681
           GLVYD T DDY+ FLC+ GY    ++  +     C      +  +LNYPSI+   P  + 
Sbjct: 635 GLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRR 694

Query: 682 G-APFTINRRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFNSVGEEKAFKLVFQCNSK 741
           G    T+ R   NVG +   Y AR++ P+GV VTV+P  L F S  + +++ +    N++
Sbjct: 695 GLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTR 754

Query: 742 ---LQRQGYVFGTLVWSD-GKHFVRSPIAV 753
              L   G VFG++ W D GKH VRSPI V
Sbjct: 755 NVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

BLAST of Tan0009395 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 580.5 bits (1495), Expect = 2.0e-165
Identity = 331/768 (43.10%), Postives = 454/768 (59.11%), Query Frame = 0

Query: 10  SSYIVYLGTHSFGSNPSIYDTQLATESQYEILGTVMESKMAAKESIIYSYNRYINGFAAV 69
           S+ + Y+      + PSI+ T       +    + + S  ++  SII++Y+   +GF+A 
Sbjct: 23  SNSLTYIVHVDHEAKPSIFPT------HFHWYTSSLASLTSSPPSIIHTYDTVFHGFSAR 82

Query: 70  LDEKEATALAKNPNVVSVFENKERKLHTTRSWRFLGME--DDGGIPSNSIWKAARFGEDT 129
           L  ++A+ L  +P+V+SV   + R LHTTRS  FLG+   D  G+   S      FG D 
Sbjct: 83  LTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEES-----DFGSDL 142

Query: 130 IIGNLDTGAWPESKSFNDAGYGPVPSRWRGACEGGSNF---QCNKKLIGARYFYQGFAMT 189
           +IG +DTG WPE  SF+D G GPVP +W+G C    +F    CN+KL+GAR+F  G+  T
Sbjct: 143 VIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEAT 202

Query: 190 HGPLN--ISLHTARDHEGHGSHTLSTAGGNFVPGVNVFGNGNGTAKGGSPKARLAAYKVC 249
           +G +N      + RD +GHG+HT S + G +V   +  G  +G A G +PKARLAAYKVC
Sbjct: 203 NGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVC 262

Query: 250 WPSNKDGGCFDSDILAGFEAAISDGVDVLSVSLGAAAQEFAYDSLSIGAFHAIQQGIVVV 309
           W S    GC+DSDILA F+ A++DGVDV+S+S+G     +  D+++IGAF AI +GI V 
Sbjct: 263 WNS----GCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVS 322

Query: 310 CSAGNDGPSPGTVSNVSPWMFTVGASSIDRDFSSYVALGNKKHFKGSSL-SSKGLPGGKF 369
            SAGN GP   TV+NV+PWM TVGA +IDRDF + V LGN K   G S+    GL  G+ 
Sbjct: 323 ASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRM 382

Query: 370 YPLINAVEAKAGNASDTLAQLCEEGSLDPTKAKGKIIVCLRGDNGRVDKGFEVLRVGGVG 429
           YPL+       G+     + LC EGSLDP   KGKI++C RG N R  KG  V + GG+G
Sbjct: 383 YPLVYGGSLLGGDGYS--SSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLG 442

Query: 430 MILANDEKDGSTIMVDPHLLPASHVSYVDGLSIAQYIN------STKTPVAFLTHVRTEL 489
           MI+AN   DG  ++ D H+LPA+ V    G  I +YI+      S+K P A +    T L
Sbjct: 443 MIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRL 502

Query: 490 GIKPSPVMASFSSRGPNPITESVIKPDITAPGVSIIASVTEDVTATDLPFDTRRVPFNVE 549
           GI+P+PV+ASFS+RGPNP T  ++KPD+ APG++I+A+  + +  + +  D RR  FN+ 
Sbjct: 503 GIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNIL 562

Query: 550 SGTSMSCPHISGVVGLLKTLYPTWSPAAIKSAIMTTAKTRDNTNMHSILLDSTKVGKGTP 609
           SGTSM+CPH+SG+  LLK  +P WSPAAI+SA++TTA T DN+     ++D +     + 
Sbjct: 563 SGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSG--EPMMDESTGNTSSV 622

Query: 610 FDYGAGHVHPNNAMDPGLVYDTTIDDYLNFLCARGYNSLALKKFSNNQFVC---AEAFAI 669
            DYG+GHVHP  AMDPGLVYD T  DY+NFLC   Y    +   +  Q  C     A  +
Sbjct: 623 MDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV 682

Query: 670 TDLNYPSISVPKLQIGAPFTIN---RRVKNVGISAGTYVARVKMPKGVAVTVEPSTLQFN 729
            +LNYPS SV   Q G         R V NVG S   Y  +++ P+G  VTVEP  L F 
Sbjct: 683 GNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFR 742

Query: 730 SVGEEKAFKLVFQCNSKLQRQGYV---FGTLVWSDGKHFVRSPIAVKL 755
            VG++ +F +  +        G      G +VWSDGK  V SP+ V L
Sbjct: 743 RVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTL 771