Tan0009324 (gene) Snake gourd v1

Overview


Name	Tan0009324
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Reverse transcriptase domain-containing protein
Location	LG05: 5457987 .. 5458469 (-)
RNA-Seq Expression	Tan0009324
Synteny	Tan0009324

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGATCCAGGCACTGTCGCAAAGAATTACTTGGGGGCTTTTAGAGAGGCAAATCATCAATGTAAGGTTATTGGTAATCAAAGTAATTCTACATTTTTCAGACAAATGGAAACCACCCGCAGAGAACATTTACAAATTAAATACAGATGCAGCTCTTAAATTTCAATCTCCCGATTCTGGTTTGGGGATTGTGATCAGAGATAGTAAAGGGAAAGATATGCTTACTGCCTCTATATATGCACCAAATGCTTTTTCGGTTGATTTTGCAGAAGGAGTAGCAATAAAGGAAGGTCTTCAGCTGGCTCGCGAAATGGGTATCTGGCCGATTTTGATTGAGACAGACTCGTTACTTATCTACAATTTGCTGAGTAAAGAGGTTGAAGATGAGTCAGAAACTGGAGTCTTGATGAAAAATGTCAAAGACTCCTATTTAAGATATTTTATTTCGGCTTACAGTTTCACCCGGCGATCCGAGAATTGA

mRNA sequence

ATGAAGATCCAGGCACTGTCGCAAAGAATTACTTGGGGGCTTTTAGAGAGGCAAATCATCAATGTAAGGTTATTGGTAATCAAAGTAATTCTACATTTTTCAGACAAATGGAAACCACCCGCAGAGAACATTTACAAATTAAATACAGATGCAGCTCTTAAATTTCAATCTCCCGATTCTGGTTTGGGGATTGTGATCAGAGATAGTAAAGGGAAAGATATGCTTACTGCCTCTATATATGCACCAAATGCTTTTTCGGTTGATTTTGCAGAAGGAGTAGCAATAAAGGAAGGTCTTCAGCTGGCTCGCGAAATGGGTATCTGGCCGATTTTGATTGAGACAGACTCGTTACTTATCTACAATTTGCTGAGTAAAGAGGTTGAAGATGAGTCAGAAACTGGAGTCTTGATGAAAAATGTCAAAGACTCCTATTTAAGATATTTTATTTCGGCTTACAGTTTCACCCGGCGATCCGAGAATTGA

Coding sequence (CDS)

ATGAAGATCCAGGCACTGTCGCAAAGAATTACTTGGGGGCTTTTAGAGAGGCAAATCATCAATGTAAGGTTATTGGTAATCAAAGTAATTCTACATTTTTCAGACAAATGGAAACCACCCGCAGAGAACATTTACAAATTAAATACAGATGCAGCTCTTAAATTTCAATCTCCCGATTCTGGTTTGGGGATTGTGATCAGAGATAGTAAAGGGAAAGATATGCTTACTGCCTCTATATATGCACCAAATGCTTTTTCGGTTGATTTTGCAGAAGGAGTAGCAATAAAGGAAGGTCTTCAGCTGGCTCGCGAAATGGGTATCTGGCCGATTTTGATTGAGACAGACTCGTTACTTATCTACAATTTGCTGAGTAAAGAGGTTGAAGATGAGTCAGAAACTGGAGTCTTGATGAAAAATGTCAAAGACTCCTATTTAAGATATTTTATTTCGGCTTACAGTTTCACCCGGCGATCCGAGAATTGA

Protein sequence

MKIQALSQRITWGLLERQIINVRLLVIKVILHFSDKWKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAIKEGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFISAYSFTRRSEN

Homology

[Close]

BLAST of Tan0009324 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 109.0 bits (271), Expect = 3.8e-20
Identity = 52/124 (41.94%), Postives = 81/124 (65.32%), Query Frame = 0


Query: 37  WKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAIK 96
           W PP + IYK+NTDA+       +GLGI+IR+ +G+ M +A+ Y  N  SVD AE +   
Sbjct: 34  WLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVAV 93

Query: 97  EGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFISAYSFTR 156
           EGLQLA ++G+ P+++ETDS  I+NL S+  ED SETG ++   K+ + +   ++++F +
Sbjct: 94  EGLQLASKIGVNPVILETDSSRIFNLFSQPSEDLSETGEIVLKAKNFWTQSLHASFNFVK 153

Query: 157 RSEN 161
           R  N
Sbjct: 154 REGN 157

[Close]

BLAST of Tan0009324 vs. NCBI nr
Match: XP_022139684.1 (uncharacterized protein LOC111010533 [Momordica charantia])

HSP 1 Score: 100.9 bits (250), Expect = 1.0e-17
Identity = 55/125 (44.00%), Postives = 81/125 (64.80%), Query Frame = 0


Query: 37  WKPPAENIYKLNTDAALKFQSPDSGLG-IVIRDSKGKDMLTASIYAPNAFSVDFAEGVAI 96
           W P  E ++KL TDA+      ++GLG I+IRD +G+ + +A+ Y  +  SVD AE +A 
Sbjct: 90  WCPAEEGVFKLKTDASFSSIDFNAGLGVIIIRDHRGQVLASATKYLEHVASVDDAEALAA 149

Query: 97  KEGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFISAYSFT 156
            EGL++A E GI PIL+ETDSL IYNL +++ E  S+TG +++ VK         +YSFT
Sbjct: 150 VEGLRVAMETGISPILLETDSLRIYNLFARDKEGLSKTGSIIEYVKTHLATRLQVSYSFT 209

Query: 157 RRSEN 161
           +R+ N
Sbjct: 210 KRTGN 214

[Close]

BLAST of Tan0009324 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 95.9 bits (237), Expect = 3.4e-16
Identity = 56/130 (43.08%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 37  WKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAIK 96
           W+PPA  + K+N DAA + +S  +G+G++IRDS G   LTA      A  VD+ EG A+ 
Sbjct: 104 WRPPAAPLLKVNVDAAFRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVY 163

Query: 97  EGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFIS------ 156
           EG+ LA E G     IETDSL I+NLL+ +  D+SE GVL      S ++ F+S      
Sbjct: 164 EGILLAVEAGFIRFQIETDSLRIFNLLTTDCVDDSEVGVLC-----SVIKLFLSSHAERV 223

Query: 157 AYSFTRRSEN 161
           ++SFT R+ N
Sbjct: 224 SFSFTHRNGN 228

[Close]

BLAST of Tan0009324 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-13
Identity = 47/124 (37.90%), Postives = 69/124 (55.65%), Query Frame = 0


Query: 37   WKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAIK 96
            W+PP E IYK+NTDA+       +GLGI+I + +G+ M  A+ Y  N  SVD AE +A  
Sbjct: 991  WQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAV 1050

Query: 97   EGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFISAYSFTR 156
            EGLQLA E+G+ P L                ED SETG ++   K+ + +   ++++F +
Sbjct: 1051 EGLQLASEIGMHPAL----------------EDLSETGEIVLKAKNFWTQSLHASFNFVK 1098

Query: 157  RSEN 161
            R  N
Sbjct: 1111 REGN 1098

[Close]

BLAST of Tan0009324 vs. NCBI nr
Match: XP_022135942.1 (uncharacterized protein LOC111007775 [Momordica charantia])

HSP 1 Score: 86.7 bits (213), Expect = 2.0e-13
Identity = 50/125 (40.00%), Postives = 70/125 (56.00%), Query Frame = 0


Query: 37  WKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAIK 96
           W PP    +K+N DAA K  +  +GL I+IRDS    +L+A  +  +   V  AE +A +
Sbjct: 73  WCPPLAPFFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFITHVSDVTLAECLAAQ 132

Query: 97  EGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFI-SAYSFT 156
           EG+ LA E G+ P  IETDS  ++NLL  + EDESE GVL  +++       I   +SF 
Sbjct: 133 EGVCLAIEAGLIPFQIETDSSQVFNLLRTDCEDESEIGVLASSIRHIVSSLHIGGGFSFV 192

Query: 157 RRSEN 161
            R  N
Sbjct: 193 NREGN 197

[Close]

BLAST of Tan0009324 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.9e-20
Identity = 52/124 (41.94%), Postives = 81/124 (65.32%), Query Frame = 0


Query: 37  WKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAIK 96
           W PP + IYK+NTDA+       +GLGI+IR+ +G+ M +A+ Y  N  SVD AE +   
Sbjct: 34  WLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLENIQSVDMAEAIVAV 93

Query: 97  EGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFISAYSFTR 156
           EGLQLA ++G+ P+++ETDS  I+NL S+  ED SETG ++   K+ + +   ++++F +
Sbjct: 94  EGLQLASKIGVNPVILETDSSRIFNLFSQPSEDLSETGEIVLKAKNFWTQSLHASFNFVK 153

Query: 157 RSEN 161
           R  N
Sbjct: 154 REGN 157

[Close]

BLAST of Tan0009324 vs. ExPASy TrEMBL
Match: A0A6J1CDQ4 (uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010533 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.1e-18
Identity = 55/125 (44.00%), Postives = 81/125 (64.80%), Query Frame = 0


Query: 37  WKPPAENIYKLNTDAALKFQSPDSGLG-IVIRDSKGKDMLTASIYAPNAFSVDFAEGVAI 96
           W P  E ++KL TDA+      ++GLG I+IRD +G+ + +A+ Y  +  SVD AE +A 
Sbjct: 90  WCPAEEGVFKLKTDASFSSIDFNAGLGVIIIRDHRGQVLASATKYLEHVASVDDAEALAA 149

Query: 97  KEGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFISAYSFT 156
            EGL++A E GI PIL+ETDSL IYNL +++ E  S+TG +++ VK         +YSFT
Sbjct: 150 VEGLRVAMETGISPILLETDSLRIYNLFARDKEGLSKTGSIIEYVKTHLATRLQVSYSFT 209

Query: 157 RRSEN 161
           +R+ N
Sbjct: 210 KRTGN 214

[Close]

BLAST of Tan0009324 vs. ExPASy TrEMBL
Match: A0A6J1DBJ7 (uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018973 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.6e-16
Identity = 56/130 (43.08%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 37  WKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAIK 96
           W+PPA  + K+N DAA + +S  +G+G++IRDS G   LTA      A  VD+ EG A+ 
Sbjct: 104 WRPPAAPLLKVNVDAAFRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVY 163

Query: 97  EGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFIS------ 156
           EG+ LA E G     IETDSL I+NLL+ +  D+SE GVL      S ++ F+S      
Sbjct: 164 EGILLAVEAGFIRFQIETDSLRIFNLLTTDCVDDSEVGVLC-----SVIKLFLSSHAERV 223

Query: 157 AYSFTRRSEN 161
           ++SFT R+ N
Sbjct: 224 SFSFTHRNGN 228

[Close]

BLAST of Tan0009324 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 5.8e-14
Identity = 47/124 (37.90%), Postives = 69/124 (55.65%), Query Frame = 0


Query: 37   WKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAIK 96
            W+PP E IYK+NTDA+       +GLGI+I + +G+ M  A+ Y  N  SVD AE +A  
Sbjct: 991  WQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMAEAIAAV 1050

Query: 97   EGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFISAYSFTR 156
            EGLQLA E+G+ P L                ED SETG ++   K+ + +   ++++F +
Sbjct: 1051 EGLQLASEIGMHPAL----------------EDLSETGEIVLKAKNFWTQSLHASFNFVK 1098

Query: 157  RSEN 161
            R  N
Sbjct: 1111 REGN 1098

[Close]

BLAST of Tan0009324 vs. ExPASy TrEMBL
Match: A0A6J1C467 (uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007775 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 9.9e-14
Identity = 50/125 (40.00%), Postives = 70/125 (56.00%), Query Frame = 0


Query: 37  WKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAIK 96
           W PP    +K+N DAA K  +  +GL I+IRDS    +L+A  +  +   V  AE +A +
Sbjct: 73  WCPPLAPFFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFITHVSDVTLAECLAAQ 132

Query: 97  EGLQLAREMGIWPILIETDSLLIYNLLSKEVEDESETGVLMKNVKDSYLRYFI-SAYSFT 156
           EG+ LA E G+ P  IETDS  ++NLL  + EDESE GVL  +++       I   +SF 
Sbjct: 133 EGVCLAIEAGLIPFQIETDSSQVFNLLRTDCEDESEIGVLASSIRHIVSSLHIGGGFSFV 192

Query: 157 RRSEN 161
            R  N
Sbjct: 193 NREGN 197

[Close]

BLAST of Tan0009324 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 45.8 bits (107), Expect = 3.7e-05
Identity = 25/95 (26.32%), Postives = 46/95 (48.42%), Query Frame = 0


Query: 32  HFSDKWKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAE 91
           + S +WK P     K NTDA  + ++P  G+G ++R+  G  +   +   P   +V  AE
Sbjct: 132 NLSVQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAE 191

Query: 92  GVAIKEGLQLAREMGIWPILIETDSLLIYNLLSKE 127
             A++  +          I+ E+D+  + NLL+ +
Sbjct: 192 LEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSD 226

[Close]

BLAST of Tan0009324 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 41.2 bits (95), Expect = 9.2e-04
Identity = 24/91 (26.37%), Postives = 43/91 (47.25%), Query Frame = 0


Query: 36  KWKPPAENIYKLNTDAALKFQSPDSGLGIVIRDSKGKDMLTASIYAPNAFSVDFAEGVAI 95
           +W+PP     K NTDA     +   G+G V+R+ KG+     +   P   SV  AE  A+
Sbjct: 419 RWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAM 478

Query: 96  KEGLQLAREMGIWPILIETDSLLIYNLLSKE 127
           +  +          ++ E+DS ++  +L+ +
Sbjct: 479 RWAVLSLSRFQYNYVIFESDSQVLIEILNND 509

The following BLAST results are available for this feature:

BLAST of Tan0009324 vs.


Match Name	E-value	Identity	Description

BLAST of Tan0009324 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140628.1	3.8e-20	41.94	uncharacterized protein LOC111011237 [Momordica charantia]	[more]
XP_022139684.1	1.0e-17	44.00	uncharacterized protein LOC111010533 [Momordica charantia]	[more]
XP_022150944.1	3.4e-16	43.08	uncharacterized protein LOC111018973 [Momordica charantia]	[more]
XP_022150918.1	1.2e-13	37.90	uncharacterized protein LOC111018954 [Momordica charantia]	[more]
XP_022135942.1	2.0e-13	40.00	uncharacterized protein LOC111007775 [Momordica charantia]	[more]

BLAST of Tan0009324 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIF1	1.9e-20	41.94	uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1CDQ4	5.1e-18	44.00	uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6J1DBJ7	1.6e-16	43.08	uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]
A0A6J1DAR4	5.8e-14	37.90	uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]
A0A6J1C467	9.9e-14	40.00	uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007...	[more]

BLAST of Tan0009324 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	3.7e-05	26.32	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G29090.1	9.2e-04	26.37	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 44..156 e-value: 7.6E-10 score: 40.9
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 48..160 e-value: 1.1E-16 score: 60.8
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 35..160
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 35..160
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 47..160 e-value: 3.18333E-13 score: 60.7908
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 44..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0009324.1	Tan0009324.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity