Tan0008832 (gene) Snake gourd v1

Overview


Name	Tan0008832
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calcium-binding EF-hand family protein
Location	LG01: 22123164 .. 22124286 (-)
RNA-Seq Expression	Tan0008832
Synteny	Tan0008832

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTCCAAAAAAAATTTCCCGTTTCTTAAAACAAAATCGAAAGATAAAACAAAAAATACGTCACCTCCCACACTCAGGCGACCGTCAAAGGGAAAGGAAAACAGAAAGAAAAATCAGAGCTCTGTGAAGATTCAAACCTAAATCGAAACCAGAGATGGGCAAAGATCTGAGCGATGATCAGAAATCCTCCATGAGAGAGGCCTTCACCCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCCGAGGAGAATCTCACTGCTCCCTTTGATTTTAATCGCTTCCTCGAGATCATGTCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTTCGTGACGCCTTTAAGGTTCTCGATAAGGACAATACCGGCTATGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATAGGTGAGAAGCTGGAGCCGTCGGAGTTTGATGAGTGGATTCGAGAAGTGGAGGTCGGATCGGATGGTCGAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGAGGAGGGAAGTAATGGAAAATTGGTTTTGTGTGCTTTACTCGAATGCTTTTTGAAGTAATTTGTGGCGATCTTTGTTGTTTTTTTTTTTCTTTTTTTTTTCTTTCCAATTACTGTTAATTGGATTCAGATTACAAGACTTGCTTGGATTTTGGTGGGATGGATGCGGGTGATGTTCGCATACTTTCCCAAGTAATGCTAATATTATTTGGAAAGATTTAGGATTATGATGGGATGAATATGTGTGTATTGAAAACTATGGTTTTCTGGGTAACGTAGAATTTGGTGATTTGTATGTGTTTCGTAGCTTAAATTGCGAAAAAATTGATTAGTTTCTCCCACGATTTATTGTCTTTCGATGGTTTATATGTCTGCGGGCTCTTAAAGTTAGAGAAACTGAGATGGTGCCTTTATGTTCACCATGCCTTGTTTTAGTTTGTAAGATTTGAAAGATTAATTAATCTTCTCTTGTTTCATTTGGTGTAGCCTTTTCTTTCTATATTAACCTCTTGAGATAAGAGGCCTGAC

mRNA sequence

ATTTCCAAAAAAAATTTCCCGTTTCTTAAAACAAAATCGAAAGATAAAACAAAAAATACGTCACCTCCCACACTCAGGCGACCGTCAAAGGGAAAGGAAAACAGAAAGAAAAATCAGAGCTCTGTGAAGATTCAAACCTAAATCGAAACCAGAGATGGGCAAAGATCTGAGCGATGATCAGAAATCCTCCATGAGAGAGGCCTTCACCCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCCGAGGAGAATCTCACTGCTCCCTTTGATTTTAATCGCTTCCTCGAGATCATGTCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTTCGTGACGCCTTTAAGGTTCTCGATAAGGACAATACCGGCTATGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATAGGTGAGAAGCTGGAGCCGTCGGAGTTTGATGAGTGGATTCGAGAAGTGGAGGTCGGATCGGATGGTCGAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGAGGAGGGAAGTAATGGAAAATTGGTTTTGTGTGCTTTACTCGAATGCTTTTTGAAGTAATTTGTGGCGATCTTTGTTGTTTTTTTTTTTCTTTTTTTTTTCTTTCCAATTACTGTTAATTGGATTCAGATTACAAGACTTGCTTGGATTTTGGTGGGATGGATGCGGGTGATGTTCGCATACTTTCCCAAGTAATGCTAATATTATTTGGAAAGATTTAGGATTATGATGGGATGAATATGTGTGTATTGAAAACTATGGTTTTCTGGGTAACGTAGAATTTGGTGATTTGTATGTGTTTCGTAGCTTAAATTGCGAAAAAATTGATTAGTTTCTCCCACGATTTATTGTCTTTCGATGGTTTATATGTCTGCGGGCTCTTAAAGTTAGAGAAACTGAGATGGTGCCTTTATGTTCACCATGCCTTGTTTTAGTTTGTAAGATTTGAAAGATTAATTAATCTTCTCTTGTTTCATTTGGTGTAGCCTTTTCTTTCTATATTAACCTCTTGAGATAAGAGGCCTGAC

Coding sequence (CDS)

ATGGGCAAAGATCTGAGCGATGATCAGAAATCCTCCATGAGAGAGGCCTTCACCCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCCGAGGAGAATCTCACTGCTCCCTTTGATTTTAATCGCTTCCTCGAGATCATGTCCAAGCACATGAAGCCCGAGCCCTTCGATCGCCAGCTTCGTGACGCCTTTAAGGTTCTCGATAAGGACAATACCGGCTATGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATAGGTGAGAAGCTGGAGCCGTCGGAGTTTGATGAGTGGATTCGAGAAGTGGAGGTCGGATCGGATGGTCGAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

Protein sequence

MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFDEWIREVEVGSDGRIRYEDFIARMVAK

Homology

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BLAST of Tan0008832 vs. ExPASy Swiss-Prot
Match: Q94AZ4 (Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 4.0e-67
Identity = 124/148 (83.78%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           MGKD LSDDQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA ENL+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  APFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGSDGRIRYEDFIARMVAK 148
           FDEWI+EV+VGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Tan0008832 vs. ExPASy Swiss-Prot
Match: Q8VZ50 (Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.4e-64
Identity = 119/148 (80.41%), Postives = 139/148 (93.92%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           M KD LS+DQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  ENL+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  APFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGSDGRIRYEDFIARMVAK 148
           FDEWI+EV+VGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Tan0008832 vs. ExPASy Swiss-Prot
Match: Q84VG0 (Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 GN=CML7 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 8.1e-60
Identity = 110/146 (75.34%), Postives = 130/146 (89.04%), Query Frame = 0


Query: 2   GKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTAP 61
           GK+LS++Q +SMREAF+LFDTDGDGRIAPSELG+LMRSLGGNPTQAQL+ I A+E LTAP
Sbjct: 3   GKELSEEQVASMREAFSLFDTDGDGRIAPSELGVLMRSLGGNPTQAQLRDIAAQEKLTAP 62

Query: 62  FDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFD 121
           FDF RFL++M  H++PEPFDR LRDAF+VLDKD +G V V++LRH+LTSIGEKLEP EFD
Sbjct: 63  FDFPRFLDLMRAHLRPEPFDRPLRDAFRVLDKDASGTVSVADLRHVLTSIGEKLEPHEFD 122

Query: 122 EWIREVEVGSDGRIRYEDFIARMVAK 148
           EWIREV+V  DG IRY+DFI R+VAK
Sbjct: 123 EWIREVDVAPDGTIRYDDFIRRIVAK 148

[Close]

BLAST of Tan0008832 vs. ExPASy Swiss-Prot
Match: Q03509 (Calmodulin-6 OS=Arabidopsis thaliana OX=3702 GN=CAM6 PE=1 SV=2)

HSP 1 Score: 147.9 bits (372), Expect = 9.0e-35
Identity = 72/149 (48.32%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVEVGSDGRIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of Tan0008832 vs. ExPASy Swiss-Prot
Match: P62200 (Calmodulin-1/11/16 OS=Daucus carota OX=4039 GN=CAM-1 PE=2 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 1.2e-34
Identity = 72/149 (48.32%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGSDGRIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of Tan0008832 vs. NCBI nr
Match: XP_038876882.1 (probable calcium-binding protein CML13 [Benincasa hispida])

HSP 1 Score: 294.7 bits (753), Expect = 4.5e-76
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDGRIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. NCBI nr
Match: XP_004147942.1 (probable calcium-binding protein CML13 [Cucumis sativus] >KGN55913.1 hypothetical protein Csa_011609 [Cucumis sativus])

HSP 1 Score: 287.7 bits (735), Expect = 5.5e-74
Identity = 143/147 (97.28%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTGYV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. NCBI nr
Match: XP_023540891.1 (probable calcium-binding protein CML13 [Cucurbita pepo subsp. pepo] >KAG6596839.1 putative calcium-binding protein CML13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028366.1 putative calcium-binding protein CML13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 286.6 bits (732), Expect = 1.2e-73
Identity = 143/147 (97.28%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLS+DQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKPE FDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVG DG+IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. NCBI nr
Match: XP_008448907.1 (PREDICTED: probable calcium-binding protein CML13 [Cucumis melo] >QNH87118.1 CML13 [Cucumis melo subsp. melo] >TYK19478.1 putative calcium-binding protein CML13 [Cucumis melo var. makuwa])

HSP 1 Score: 286.2 bits (731), Expect = 1.6e-73
Identity = 142/147 (96.60%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. NCBI nr
Match: XP_022152721.1 (probable calcium-binding protein CML13 [Momordica charantia])

HSP 1 Score: 285.8 bits (730), Expect = 2.1e-73
Identity = 140/147 (95.24%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LT+
Sbjct: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL++MSKHMKPEPFDRQLRDAFKVLDKDNTG+VLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREV+VGSDGRIRYEDFIARMVAK
Sbjct: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. ExPASy TrEMBL
Match: A0A0A0L2F2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036390 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.7e-74
Identity = 143/147 (97.28%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTGYV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. ExPASy TrEMBL
Match: A0A5D3D7C6 (Putative calcium-binding protein CML13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001330 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 7.8e-74
Identity = 142/147 (96.60%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. ExPASy TrEMBL
Match: A0A7G7Y0U3 (CML13 OS=Cucumis melo subsp. melo OX=412675 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 7.8e-74
Identity = 142/147 (96.60%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. ExPASy TrEMBL
Match: A0A1S3BLQ3 (probable calcium-binding protein CML13 OS=Cucumis melo OX=3656 GN=LOC103490931 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 7.8e-74
Identity = 142/147 (96.60%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREVEVGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. ExPASy TrEMBL
Match: A0A6J1DIL4 (probable calcium-binding protein CML13 OS=Momordica charantia OX=3673 GN=LOC111020377 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.0e-73
Identity = 140/147 (95.24%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LT+
Sbjct: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60

Query: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL++MSKHMKPEPFDRQLRDAFKVLDKDNTG+VLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 148
           DEWIREV+VGSDGRIRYEDFIARMVAK
Sbjct: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of Tan0008832 vs. TAIR 10
Match: AT1G12310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 255.4 bits (651), Expect = 2.8e-68
Identity = 124/148 (83.78%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           MGKD LSDDQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA ENL+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  APFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGSDGRIRYEDFIARMVAK 148
           FDEWI+EV+VGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Tan0008832 vs. TAIR 10
Match: AT1G62820.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 246.9 bits (629), Expect = 1.0e-65
Identity = 119/148 (80.41%), Postives = 139/148 (93.92%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           M KD LS+DQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  ENL+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  APFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGSDGRIRYEDFIARMVAK 148
           FDEWI+EV+VGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Tan0008832 vs. TAIR 10
Match: AT5G21274.1 (calmodulin 6 )

HSP 1 Score: 147.9 bits (372), Expect = 6.4e-36
Identity = 72/149 (48.32%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVEVGSDGRIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of Tan0008832 vs. TAIR 10
Match: AT3G43810.1 (calmodulin 7 )

HSP 1 Score: 147.5 bits (371), Expect = 8.3e-36
Identity = 72/149 (48.32%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGSDGRIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of Tan0008832 vs. TAIR 10
Match: AT2G41110.1 (calmodulin 2 )

HSP 1 Score: 146.4 bits (368), Expect = 1.9e-35
Identity = 71/149 (47.65%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DDQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGSDGRIRYEDFIARMVAK 148
           E DE I+E +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIKEADVDGDGQINYEEFVKVMMAK 149

The following BLAST results are available for this feature:

BLAST of Tan0008832 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AZ4	4.0e-67	83.78	Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 ...	[more]
Q8VZ50	1.4e-64	80.41	Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 ...	[more]
Q84VG0	8.1e-60	75.34	Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q03509	9.0e-35	48.32	Calmodulin-6 OS=Arabidopsis thaliana OX=3702 GN=CAM6 PE=1 SV=2	[more]
P62200	1.2e-34	48.32	Calmodulin-1/11/16 OS=Daucus carota OX=4039 GN=CAM-1 PE=2 SV=2	[more]

BLAST of Tan0008832 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876882.1	4.5e-76	100.00	probable calcium-binding protein CML13 [Benincasa hispida]	[more]
XP_004147942.1	5.5e-74	97.28	probable calcium-binding protein CML13 [Cucumis sativus] >KGN55913.1 hypothetica...	[more]
XP_023540891.1	1.2e-73	97.28	probable calcium-binding protein CML13 [Cucurbita pepo subsp. pepo] >KAG6596839....	[more]
XP_008448907.1	1.6e-73	96.60	PREDICTED: probable calcium-binding protein CML13 [Cucumis melo] >QNH87118.1 CML...	[more]
XP_022152721.1	2.1e-73	95.24	probable calcium-binding protein CML13 [Momordica charantia]	[more]

BLAST of Tan0008832 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2F2	2.7e-74	97.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036390 PE=4 SV=1	[more]
A0A5D3D7C6	7.8e-74	96.60	Putative calcium-binding protein CML13 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A7G7Y0U3	7.8e-74	96.60	CML13 OS=Cucumis melo subsp. melo OX=412675 PE=2 SV=1	[more]
A0A1S3BLQ3	7.8e-74	96.60	probable calcium-binding protein CML13 OS=Cucumis melo OX=3656 GN=LOC103490931 P...	[more]
A0A6J1DIL4	1.0e-73	95.24	probable calcium-binding protein CML13 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Tan0008832 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12310.1	2.8e-68	83.78	Calcium-binding EF-hand family protein	[more]
AT1G62820.1	1.0e-65	80.41	Calcium-binding EF-hand family protein	[more]
AT5G21274.1	6.4e-36	48.32	calmodulin 6	[more]
AT3G43810.1	8.3e-36	48.32	calmodulin 7	[more]
AT2G41110.1	1.9e-35	47.65	calmodulin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 83..111 e-value: 4.7E-4 score: 29.5 coord: 12..40 e-value: 1.2E-4 score: 31.5 coord: 119..147 e-value: 2.3 score: 14.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 82..144 e-value: 1.7E-13 score: 50.8
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 13..41 e-value: 2.1E-6 score: 27.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 8..43 score: 12.979158
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 12.281773
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 14..72 e-value: 6.93737E-9 score: 47.5425
None	No IPR available	GENE3D	1.10.238.10		coord: 81..145 e-value: 1.2E-18 score: 69.0
None	No IPR available	GENE3D	1.10.238.10		coord: 3..80 e-value: 1.8E-20 score: 74.9
None	No IPR available	PANTHER	PTHR23050:SF412	SUBFAMILY NOT NAMED	coord: 1..147
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..147
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0008832.1	Tan0008832.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding