Tan0008805 (gene) Snake gourd v1

Overview
NameTan0008805
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCalcium-binding EF hand family protein
LocationLG02: 86508657 .. 86509327 (+)
RNA-Seq ExpressionTan0008805
SyntenyTan0008805
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTATTCAAGCTAAATTATAGTCTTTGTTTAAAAATGTCTGCCTCCCAACTCCAAGCTTGGGCTAAGGATGAAGCAAATCAATTGATGAAAAAGTACGATAAAAATGGTGATGGTGTTCTCACTAAAGAAGAGCTACAATCTTTTCTCCACGACGCTCGAAGTTCCAAGCCACTCAACAATCAAGTGTTTATATCTAAGATTCCAAAAGCTAATAATAATGTTGCGCACAAGCTTGGCTCGAACGCTCATATGAATCCAAGAAAGCAATTACACGACAAAGGTAAGAAATCCTCGACAAAAGTTCTTAATGGAGAGACATCTAATATGCAACTTAGTCGAGAGCAGATAAAAGAGGTTTTCAAATGTCATGATAGCGACAAGGATGGTTTTCTCAACATAAAGGAGATCACAAAAGCTTTTGGTTCTCTAGGCTCAATTATTCCAATCTACAAAGCTCACTATGGTTTGGCTTATGCTGATGCTAATAAAGATGGTCTTATTAGCGAGGCTGAGCTTGACAAACTCATAGATTATGCATGTAAAGTCATTAAAAAGAAATGAACTTAGTTATGAGGTGCTTTCTTAGAATTCGATGTATGAAGGTAATTGTAACTTGATTTAAATCATTGAAAAAAATGATTTGAAAATAAAATAAAGAGAAGTGTGTT

mRNA sequence

ATTTTATTCAAGCTAAATTATAGTCTTTGTTTAAAAATGTCTGCCTCCCAACTCCAAGCTTGGGCTAAGGATGAAGCAAATCAATTGATGAAAAAGTACGATAAAAATGGTGATGGTGTTCTCACTAAAGAAGAGCTACAATCTTTTCTCCACGACGCTCGAAGTTCCAAGCCACTCAACAATCAAGTGTTTATATCTAAGATTCCAAAAGCTAATAATAATGTTGCGCACAAGCTTGGCTCGAACGCTCATATGAATCCAAGAAAGCAATTACACGACAAAGGTAAGAAATCCTCGACAAAAGTTCTTAATGGAGAGACATCTAATATGCAACTTAGTCGAGAGCAGATAAAAGAGGTTTTCAAATGTCATGATAGCGACAAGGATGGTTTTCTCAACATAAAGGAGATCACAAAAGCTTTTGGTTCTCTAGGCTCAATTATTCCAATCTACAAAGCTCACTATGGTTTGGCTTATGCTGATGCTAATAAAGATGGTCTTATTAGCGAGGCTGAGCTTGACAAACTCATAGATTATGCATGTAAAGTCATTAAAAAGAAATGAACTTAGTTATGAGGTGCTTTCTTAGAATTCGATGTATGAAGGTAATTGTAACTTGATTTAAATCATTGAAAAAAATGATTTGAAAATAAAATAAAGAGAAGTGTGTT

Coding sequence (CDS)

ATGTCTGCCTCCCAACTCCAAGCTTGGGCTAAGGATGAAGCAAATCAATTGATGAAAAAGTACGATAAAAATGGTGATGGTGTTCTCACTAAAGAAGAGCTACAATCTTTTCTCCACGACGCTCGAAGTTCCAAGCCACTCAACAATCAAGTGTTTATATCTAAGATTCCAAAAGCTAATAATAATGTTGCGCACAAGCTTGGCTCGAACGCTCATATGAATCCAAGAAAGCAATTACACGACAAAGGTAAGAAATCCTCGACAAAAGTTCTTAATGGAGAGACATCTAATATGCAACTTAGTCGAGAGCAGATAAAAGAGGTTTTCAAATGTCATGATAGCGACAAGGATGGTTTTCTCAACATAAAGGAGATCACAAAAGCTTTTGGTTCTCTAGGCTCAATTATTCCAATCTACAAAGCTCACTATGGTTTGGCTTATGCTGATGCTAATAAAGATGGTCTTATTAGCGAGGCTGAGCTTGACAAACTCATAGATTATGCATGTAAAGTCATTAAAAAGAAATGA

Protein sequence

MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFLNIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKKK
Homology
BLAST of Tan0008805 vs. ExPASy Swiss-Prot
Match: P25071 (Calmodulin-like protein 12 OS=Arabidopsis thaliana OX=3702 GN=CML12 PE=1 SV=3)

HSP 1 Score: 46.6 bits (109), Expect = 3.4e-04
Identity = 40/166 (24.10%), Postives = 76/166 (45.78%), Query Frame = 0

Query: 13  EANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVAHKLGSNAH 72
           E  +  + +DKNGDG +TK+EL++ +     ++   +   +      + +         +
Sbjct: 101 EYRESFRLFDKNGDGSITKKELRTVMFSLGKNRTKADLQDMMNEVDLDGDGTIDFPEFLY 160

Query: 73  MNPRKQLHDKGKKSSTKVLNGETSNMQLSREQI---KEVFKCHDSDKDGFLNIKEITKAF 132
           +  + Q HD+  + + K +     + QL+ +QI   +E F+  D + DG++ + E+    
Sbjct: 161 LMAKNQGHDQAPRHTKKTM----VDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTM 220

Query: 133 GSLGSIIPIYKAHYGLAYADANKDGLISEAEL-----DKLIDYACK 171
            SLG      +    +  ADA+ DG IS +E       K+ID   K
Sbjct: 221 RSLGETQTKAELQDMINEADADGDGTISFSEFVCVMTGKMIDTQSK 262

BLAST of Tan0008805 vs. ExPASy Swiss-Prot
Match: Q9AWK2 (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 GN=CML11 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.7e-04
Identity = 36/156 (23.08%), Postives = 64/156 (41.03%), Query Frame = 0

Query: 13  EANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVAHKLGSNAH 72
           E  ++ + +D+NGDG LT+ EL S L         +    + +    N+N   +      
Sbjct: 64  ELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTNSNGLIEFSEFVA 123

Query: 73  MNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFLNIKEITKAFGSL 132
           +   + L+D+                  S +QI+ +F   D D +GF+   E+  +   L
Sbjct: 124 LVAPELLYDRA---------------PYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKL 183

Query: 133 GSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYA 169
           G  + + +    +  AD + DG IS  E  + I  A
Sbjct: 184 GHALTVKELTGMIKEADTDGDGRISFQEFSRAITAA 204

BLAST of Tan0008805 vs. ExPASy Swiss-Prot
Match: Q9LQN4 (Probable calcium-binding protein CML17 OS=Arabidopsis thaliana OX=3702 GN=CML17 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.7e-04
Identity = 41/157 (26.11%), Postives = 73/157 (46.50%), Query Frame = 0

Query: 12  DEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVAHKLGSNA 71
           +E  ++ + +D+N DG LT+ EL S L  A   KP  +Q F + I KA+           
Sbjct: 15  NELREIFRSFDRNKDGSLTQLELGSLLR-ALGVKPSPDQ-FETLIDKADTK--------- 74

Query: 72  HMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFLNIKEITKAFGS 131
             N   +  +     S ++L+        + EQ+  +F+  D+D +GF+   E+  +   
Sbjct: 75  -SNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAK 134

Query: 132 LGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYA 169
           LG  + + +    +  AD++ DG I+  E  K I+ A
Sbjct: 135 LGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSA 159

BLAST of Tan0008805 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 7.5e-04
Identity = 41/156 (26.28%), Postives = 74/156 (47.44%), Query Frame = 0

Query: 5   QLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVA 64
           QL+     +  +L +++D NGDG LT+ EL + L  +   +P  ++V            A
Sbjct: 17  QLRGSQLKQLRELFRRFDMNGDGSLTQLELAALLR-SLGLRPTGDEVH-----------A 76

Query: 65  HKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFLNIKE 124
              G +A+ N   +  D+   +   VL  +T    + + Q+ EVF+  D D +GF++  E
Sbjct: 77  LLAGMDANGNGSVEF-DELAAAIAPVLTTQTH--LVDQAQLLEVFRAFDRDGNGFISAAE 136

Query: 125 ITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAE 161
           + ++   LG  +   +    +  AD + DG+IS  E
Sbjct: 137 LARSMARLGQPLTFEELTRMMRDADTDGDGVISFKE 157

BLAST of Tan0008805 vs. NCBI nr
Match: XP_023002688.1 (calcium-binding protein H-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 246.5 bits (628), Expect = 1.7e-61
Identity = 126/175 (72.00%), Postives = 145/175 (82.86%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPL-NNQVFISKIPKA 60
           MSAS+ Q WA+DEAN+LMKKYD NGDG+LTKEELQ FLH AR SK L NNQ F+ KIPKA
Sbjct: 1   MSASKFQTWAEDEANRLMKKYDTNGDGMLTKEELQVFLHKARGSKQLNNNQGFVFKIPKA 60

Query: 61  NNNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGF 120
           NNNVAH+  SN+HM  +KQ+ DKGKKSSTK  +GE S ++LSREQ+KE+FK HDS+KDGF
Sbjct: 61  NNNVAHEHSSNSHMKHKKQVQDKGKKSSTKFFDGEMSKLRLSREQMKEIFKNHDSNKDGF 120

Query: 121 LNIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKK 175
           L+IKE+TKAFG LGSI+P  KAHY +AYAD N DGLISE ELDKLIDYA K I K
Sbjct: 121 LDIKEVTKAFGFLGSILPFNKAHYAMAYADTNHDGLISEFELDKLIDYASKFINK 175

BLAST of Tan0008805 vs. NCBI nr
Match: XP_023538534.1 (putative calmodulin-like protein 6 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.7 bits (626), Expect = 2.9e-61
Identity = 126/176 (71.59%), Postives = 144/176 (81.82%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPL-NNQVFISKIPKA 60
           M AS+ Q WA+DEAN+LMKKYD NGDG+LTKEELQ FLH AR S  L NNQ F+SKIPKA
Sbjct: 1   MPASKFQTWAEDEANRLMKKYDTNGDGMLTKEELQVFLHKARGSTQLNNNQGFVSKIPKA 60

Query: 61  NNNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGF 120
           +NNV HK  SN+HM  +KQ+ DKGKKSSTK L+GE S +QLSREQ+KE+FK HDS+KDGF
Sbjct: 61  SNNVVHKHSSNSHMKHKKQVQDKGKKSSTKFLDGEMSKLQLSREQMKEIFKNHDSNKDGF 120

Query: 121 LNIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKKK 176
           L+IKE+TKAFG LGSI+P  KAHY + YAD N DGLISE ELDKLIDYA K I KK
Sbjct: 121 LDIKEVTKAFGFLGSILPFNKAHYAMTYADTNHDGLISEFELDKLIDYASKFINKK 176

BLAST of Tan0008805 vs. NCBI nr
Match: XP_038886759.1 (probable calcium-binding protein CML11 [Benincasa hispida])

HSP 1 Score: 242.7 bits (618), Expect = 2.4e-60
Identity = 124/177 (70.06%), Postives = 144/177 (81.36%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQ--VFISKIPK 60
           MS SQLQAWA++EAN+L+KKYD+NGDGVL KEELQSFLHD  SS  LNN   V    IPK
Sbjct: 1   MSVSQLQAWAENEANELIKKYDRNGDGVLAKEELQSFLHDTCSSTQLNNTRCVLSRMIPK 60

Query: 61  ANNNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDG 120
            NNN+ HKL SN HMN +KQL DK + SSTK L+ + SN+  SREQIKE+FK HDS+KDG
Sbjct: 61  TNNNLVHKLSSNTHMNQKKQLRDKIQNSSTKALDRKQSNIPPSREQIKEIFKYHDSNKDG 120

Query: 121 FLNIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKKK 176
           FLNI+E+TK FGSLGS+IP YKAHY +AYADA+KDGLISE ELD+LIDY  K+IKKK
Sbjct: 121 FLNIRELTKTFGSLGSVIPFYKAHYAMAYADADKDGLISEVELDELIDYTNKIIKKK 177

BLAST of Tan0008805 vs. NCBI nr
Match: XP_022951134.1 (uncharacterized protein LOC111454074 isoform X1 [Cucurbita moschata])

HSP 1 Score: 241.5 bits (615), Expect = 5.4e-60
Identity = 126/176 (71.59%), Postives = 144/176 (81.82%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPL-NNQVFISKIPKA 60
           M AS+ Q  A+DEAN+LMKKYD NGDG+LTKEELQ FLH AR S  L NNQ F+SKIPKA
Sbjct: 1   MPASKFQTCAEDEANRLMKKYDTNGDGMLTKEELQVFLHKARGSTQLNNNQGFVSKIPKA 60

Query: 61  NNNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGF 120
           +NNVAHK  SN+HM  +KQ+ DKGKKSSTK L+GE S +QLSREQ+KE+FK HDS+KDGF
Sbjct: 61  SNNVAHKHSSNSHMKHKKQVQDKGKKSSTKFLDGEMSKLQLSREQMKEIFKNHDSNKDGF 120

Query: 121 LNIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKKK 176
           L+IKE+TKAF  LGSI+P  KAHY +AYAD N DGLISE ELDKLIDYA K I KK
Sbjct: 121 LDIKEVTKAFSFLGSILPFNKAHYAMAYADTNHDGLISEFELDKLIDYASKFINKK 176

BLAST of Tan0008805 vs. NCBI nr
Match: XP_023002691.1 (probable calcium-binding protein CML11 [Cucurbita maxima])

HSP 1 Score: 240.0 bits (611), Expect = 1.6e-59
Identity = 121/175 (69.14%), Postives = 141/175 (80.57%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKAN 60
           MS S LQ WA++EAN+LMKKYDKNGDG+LTKEELQ FL D R S  LN Q F+SKIPK N
Sbjct: 1   MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSKIPKTN 60

Query: 61  NNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFL 120
           +  AHKL SN H NP+ Q+ DK  KSSTKVL+ + SN+ LSREQIK++FK +DSDKDGFL
Sbjct: 61  DKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDGFL 120

Query: 121 NIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKKK 176
           NI+E+TKAF  LGSI P YKA Y + +ADAN DGLISE EL+KLIDYACK+IKKK
Sbjct: 121 NIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISETELEKLIDYACKIIKKK 175

BLAST of Tan0008805 vs. ExPASy TrEMBL
Match: A0A6J1KUA8 (calcium-binding protein H-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496471 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 8.2e-62
Identity = 126/175 (72.00%), Postives = 145/175 (82.86%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPL-NNQVFISKIPKA 60
           MSAS+ Q WA+DEAN+LMKKYD NGDG+LTKEELQ FLH AR SK L NNQ F+ KIPKA
Sbjct: 1   MSASKFQTWAEDEANRLMKKYDTNGDGMLTKEELQVFLHKARGSKQLNNNQGFVFKIPKA 60

Query: 61  NNNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGF 120
           NNNVAH+  SN+HM  +KQ+ DKGKKSSTK  +GE S ++LSREQ+KE+FK HDS+KDGF
Sbjct: 61  NNNVAHEHSSNSHMKHKKQVQDKGKKSSTKFFDGEMSKLRLSREQMKEIFKNHDSNKDGF 120

Query: 121 LNIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKK 175
           L+IKE+TKAFG LGSI+P  KAHY +AYAD N DGLISE ELDKLIDYA K I K
Sbjct: 121 LDIKEVTKAFGFLGSILPFNKAHYAMAYADTNHDGLISEFELDKLIDYASKFINK 175

BLAST of Tan0008805 vs. ExPASy TrEMBL
Match: A0A6J1GHU7 (uncharacterized protein LOC111454074 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454074 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.6e-60
Identity = 126/176 (71.59%), Postives = 144/176 (81.82%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPL-NNQVFISKIPKA 60
           M AS+ Q  A+DEAN+LMKKYD NGDG+LTKEELQ FLH AR S  L NNQ F+SKIPKA
Sbjct: 1   MPASKFQTCAEDEANRLMKKYDTNGDGMLTKEELQVFLHKARGSTQLNNNQGFVSKIPKA 60

Query: 61  NNNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGF 120
           +NNVAHK  SN+HM  +KQ+ DKGKKSSTK L+GE S +QLSREQ+KE+FK HDS+KDGF
Sbjct: 61  SNNVAHKHSSNSHMKHKKQVQDKGKKSSTKFLDGEMSKLQLSREQMKEIFKNHDSNKDGF 120

Query: 121 LNIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKKK 176
           L+IKE+TKAF  LGSI+P  KAHY +AYAD N DGLISE ELDKLIDYA K I KK
Sbjct: 121 LDIKEVTKAFSFLGSILPFNKAHYAMAYADTNHDGLISEFELDKLIDYASKFINKK 176

BLAST of Tan0008805 vs. ExPASy TrEMBL
Match: A0A6J1KM01 (probable calcium-binding protein CML11 OS=Cucurbita maxima OX=3661 GN=LOC111496473 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 7.6e-60
Identity = 121/175 (69.14%), Postives = 141/175 (80.57%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKAN 60
           MS S LQ WA++EAN+LMKKYDKNGDG+LTKEELQ FL D R S  LN Q F+SKIPK N
Sbjct: 1   MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSKIPKTN 60

Query: 61  NNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFL 120
           +  AHKL SN H NP+ Q+ DK  KSSTKVL+ + SN+ LSREQIK++FK +DSDKDGFL
Sbjct: 61  DKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDGFL 120

Query: 121 NIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKKK 176
           NI+E+TKAF  LGSI P YKA Y + +ADAN DGLISE EL+KLIDYACK+IKKK
Sbjct: 121 NIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISETELEKLIDYACKIIKKK 175

BLAST of Tan0008805 vs. ExPASy TrEMBL
Match: A0A6J1KK82 (probable calcium-binding protein CML14 OS=Cucurbita maxima OX=3661 GN=LOC111496474 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.9e-59
Identity = 118/175 (67.43%), Postives = 143/175 (81.71%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKAN 60
           M  S LQ WA++EAN+LMKKYD+NGDGVLTK+ELQ FL D R S  LNNQ F+S+IPK N
Sbjct: 1   MFVSILQVWAENEANELMKKYDRNGDGVLTKKELQIFLRDVRGSTQLNNQGFLSRIPKTN 60

Query: 61  NNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFL 120
           +NVAHKL SNAH NP+ Q+ +K  KSSTKV +   S+++LSREQIK++FK +DSDKDGFL
Sbjct: 61  DNVAHKLSSNAHSNPKMQVQEKATKSSTKVFSENVSDVRLSREQIKKIFKYYDSDKDGFL 120

Query: 121 NIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKKK 176
           NI E+TKAF  LGSI P YKA Y +++ADAN DG+ISEAEL+KLIDYACK+I KK
Sbjct: 121 NITEVTKAFSFLGSINPFYKAQYAISFADANNDGVISEAELEKLIDYACKIIPKK 175

BLAST of Tan0008805 vs. ExPASy TrEMBL
Match: A0A6J1GIQ8 (probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC111454532 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 5.0e-59
Identity = 120/175 (68.57%), Postives = 142/175 (81.14%), Query Frame = 0

Query: 1   MSASQLQAWAKDEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKAN 60
           MS S LQAWA++EAN+LMKKYD+NGDGVLTKEELQ FL D R S  LN+Q F+SKIPK N
Sbjct: 1   MSVSILQAWAENEANELMKKYDRNGDGVLTKEELQVFLRDVRGSTQLNHQRFLSKIPKTN 60

Query: 61  NNVAHKLGSNAHMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFL 120
           +  AH+L SNAH NP+ Q+     KSSTKVL+ + SN+ LSREQIK++FK +DSDKDGFL
Sbjct: 61  DKGAHQLSSNAHTNPKIQVQGTATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDGFL 120

Query: 121 NIKEITKAFGSLGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYACKVIKKK 176
           N +E+TKAF  LGSI P YKA Y + +ADAN DGLISEAEL+KLIDYACK+IKKK
Sbjct: 121 NTREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLIDYACKIIKKK 175

BLAST of Tan0008805 vs. TAIR 10
Match: AT2G41100.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 46.6 bits (109), Expect = 2.4e-05
Identity = 40/166 (24.10%), Postives = 76/166 (45.78%), Query Frame = 0

Query: 13  EANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVAHKLGSNAH 72
           E  +  + +DKNGDG +TK+EL++ +     ++   +   +      + +         +
Sbjct: 101 EYRESFRLFDKNGDGSITKKELRTVMFSLGKNRTKADLQDMMNEVDLDGDGTIDFPEFLY 160

Query: 73  MNPRKQLHDKGKKSSTKVLNGETSNMQLSREQI---KEVFKCHDSDKDGFLNIKEITKAF 132
           +  + Q HD+  + + K +     + QL+ +QI   +E F+  D + DG++ + E+    
Sbjct: 161 LMAKNQGHDQAPRHTKKTM----VDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTM 220

Query: 133 GSLGSIIPIYKAHYGLAYADANKDGLISEAEL-----DKLIDYACK 171
            SLG      +    +  ADA+ DG IS +E       K+ID   K
Sbjct: 221 RSLGETQTKAELQDMINEADADGDGTISFSEFVCVMTGKMIDTQSK 262

BLAST of Tan0008805 vs. TAIR 10
Match: AT2G41100.3 (Calcium-binding EF hand family protein )

HSP 1 Score: 46.6 bits (109), Expect = 2.4e-05
Identity = 40/166 (24.10%), Postives = 76/166 (45.78%), Query Frame = 0

Query: 13  EANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVAHKLGSNAH 72
           E  +  + +DKNGDG +TK+EL++ +     ++   +   +      + +         +
Sbjct: 66  EYRESFRLFDKNGDGSITKKELRTVMFSLGKNRTKADLQDMMNEVDLDGDGTIDFPEFLY 125

Query: 73  MNPRKQLHDKGKKSSTKVLNGETSNMQLSREQI---KEVFKCHDSDKDGFLNIKEITKAF 132
           +  + Q HD+  + + K +     + QL+ +QI   +E F+  D + DG++ + E+    
Sbjct: 126 LMAKNQGHDQAPRHTKKTM----VDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTM 185

Query: 133 GSLGSIIPIYKAHYGLAYADANKDGLISEAEL-----DKLIDYACK 171
            SLG      +    +  ADA+ DG IS +E       K+ID   K
Sbjct: 186 RSLGETQTKAELQDMINEADADGDGTISFSEFVCVMTGKMIDTQSK 227

BLAST of Tan0008805 vs. TAIR 10
Match: AT2G41100.4 (Calcium-binding EF hand family protein )

HSP 1 Score: 46.6 bits (109), Expect = 2.4e-05
Identity = 40/166 (24.10%), Postives = 76/166 (45.78%), Query Frame = 0

Query: 13  EANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVAHKLGSNAH 72
           E  +  + +DKNGDG +TK+EL++ +     ++   +   +      + +         +
Sbjct: 101 EYRESFRLFDKNGDGSITKKELRTVMFSLGKNRTKADLQDMMNEVDLDGDGTIDFPEFLY 160

Query: 73  MNPRKQLHDKGKKSSTKVLNGETSNMQLSREQI---KEVFKCHDSDKDGFLNIKEITKAF 132
           +  + Q HD+  + + K +     + QL+ +QI   +E F+  D + DG++ + E+    
Sbjct: 161 LMAKNQGHDQAPRHTKKTM----VDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTM 220

Query: 133 GSLGSIIPIYKAHYGLAYADANKDGLISEAEL-----DKLIDYACK 171
            SLG      +    +  ADA+ DG IS +E       K+ID   K
Sbjct: 221 RSLGETQTKAELQDMINEADADGDGTISFSEFVCVMTGKMIDTQSK 262

BLAST of Tan0008805 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 45.8 bits (107), Expect = 4.1e-05
Identity = 41/157 (26.11%), Postives = 73/157 (46.50%), Query Frame = 0

Query: 12  DEANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVAHKLGSNA 71
           +E  ++ + +D+N DG LT+ EL S L  A   KP  +Q F + I KA+           
Sbjct: 15  NELREIFRSFDRNKDGSLTQLELGSLLR-ALGVKPSPDQ-FETLIDKADTK--------- 74

Query: 72  HMNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFLNIKEITKAFGS 131
             N   +  +     S ++L+        + EQ+  +F+  D+D +GF+   E+  +   
Sbjct: 75  -SNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAK 134

Query: 132 LGSIIPIYKAHYGLAYADANKDGLISEAELDKLIDYA 169
           LG  + + +    +  AD++ DG I+  E  K I+ A
Sbjct: 135 LGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSA 159

BLAST of Tan0008805 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 43.1 bits (100), Expect = 2.6e-04
Identity = 32/121 (26.45%), Postives = 57/121 (47.11%), Query Frame = 0

Query: 13  EANQLMKKYDKNGDGVLTKEELQSFLHDARSSKPLNNQVFISKIPKANNNVAHKLGSNAH 72
           E  ++ + +DKNGDG +TKEEL   L         N  ++I    K    + HK+ +N  
Sbjct: 65  ELKRVFQMFDKNGDGRITKEELNDSLE--------NLGIYIP--DKDLTQMIHKIDANGD 124

Query: 73  MNPRKQLHDKGKKSSTKVLNGETSNMQLSREQIKEVFKCHDSDKDGFLNIKEITKAFGSL 132
                   D+ +   + +++   ++ +   E +K+ F   D D DGF+ ++E+     SL
Sbjct: 125 GCVD---IDEFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASL 172

Query: 133 G 134
           G
Sbjct: 185 G 172

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P250713.4e-0424.10Calmodulin-like protein 12 OS=Arabidopsis thaliana OX=3702 GN=CML12 PE=1 SV=3[more]
Q9AWK25.7e-0423.08Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9LQN45.7e-0426.11Probable calcium-binding protein CML17 OS=Arabidopsis thaliana OX=3702 GN=CML17 ... [more]
Q6L5F47.5e-0426.28Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
XP_023002688.11.7e-6172.00calcium-binding protein H-like isoform X1 [Cucurbita maxima][more]
XP_023538534.12.9e-6171.59putative calmodulin-like protein 6 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_038886759.12.4e-6070.06probable calcium-binding protein CML11 [Benincasa hispida][more]
XP_022951134.15.4e-6071.59uncharacterized protein LOC111454074 isoform X1 [Cucurbita moschata][more]
XP_023002691.11.6e-5969.14probable calcium-binding protein CML11 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1KUA88.2e-6272.00calcium-binding protein H-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1GHU72.6e-6071.59uncharacterized protein LOC111454074 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KM017.6e-6069.14probable calcium-binding protein CML11 OS=Cucurbita maxima OX=3661 GN=LOC1114964... [more]
A0A6J1KK822.9e-5967.43probable calcium-binding protein CML14 OS=Cucurbita maxima OX=3661 GN=LOC1114964... [more]
A0A6J1GIQ85.0e-5968.57probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
Match NameE-valueIdentityDescription
AT2G41100.12.4e-0524.10Calcium-binding EF hand family protein [more]
AT2G41100.32.4e-0524.10Calcium-binding EF hand family protein [more]
AT2G41100.42.4e-0524.10Calcium-binding EF hand family protein [more]
AT1G32250.14.1e-0526.11Calcium-binding EF-hand family protein [more]
AT2G43290.12.6e-0426.45Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 104..132
e-value: 0.11
score: 21.6
coord: 13..41
e-value: 3.3E-4
score: 30.0
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 148..164
e-value: 0.019
score: 14.5
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 16..129
e-value: 8.5E-10
score: 38.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 100..135
score: 12.811786
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 9..44
score: 13.062845
NoneNo IPR availableGENE3D1.10.238.10coord: 57..168
e-value: 3.2E-11
score: 45.2
NoneNo IPR availableGENE3D1.10.238.10coord: 9..45
e-value: 5.7E-7
score: 31.0
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 88..170
NoneNo IPR availablePANTHERPTHR10891:SF911SUBFAMILY NOT NAMEDcoord: 88..170
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 22..34
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 113..125
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 149..161
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 5..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0008805.1Tan0008805.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding