Tan0008726 (gene) Snake gourd v1

Overview
NameTan0008726
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionserine/threonine-protein kinase HT1-like
LocationLG05: 84958429 .. 84960996 (-)
RNA-Seq ExpressionTan0008726
SyntenyTan0008726
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGGGTTCGGGGGGAGACTGCGACTTGGGGGCTCCGAGATTGAGACTTTCTCTTGGTGTTTCATGGGATATATTGGTTTTCAAGGGATTTTGGTTTTGATGGATATGAGATTATTGGTTCAGACTATCTTGTTCTTGATTGATCTTCATGGCTTTGATTAGCCATCTCAAGGTTCTGCTAAAGAAGAACAAGTTCTGTTTCTTTGCTTGTTTTGCTTCCTGTTTTTCTTAATTGTTGATGGGATTTGTTGAATTGGGGTTCTGGAGCTGTATGATTCAGACTAGTTTGTCTCTGAACTACCCTTTTGAATTCAAGCCTTCTTCTCAGCTGGTGAAGAAGAACAAGAAGAAAAGTAAGAAGGTTTTTGGATACGAGATCGATTAGTTTCTTTTTGTTTTTCAGTCTTTAGAAAATCATGGAGGAAGATGCTAATTCTTGGTTAAGAAGGACCAACTTTTCTCATACAATATGCTACCGTTTAGAGTCCTTACGATTGGCTTCCTTTCCTGTCACCACTCAGCCACCACTCAATTCAGTTGCGCAATCAAAAGCTGTCCCAGTTCCTTCTCATCATAGATCTACCCTTTGTTATAACCTACCCATTGAGAACAATCATGTTCCTAACAAGCAGAAGTTGTCTGTGTCTCTGCCTCCACAAACTACACTCTCGAGTGCATTTAAGGATGCACGTAAGGATCAGAAGCGATTTTCAACTCCACAACCTCGGAGGAGAGCAACCAAAAAAGAGAAGGGCAAAAGGGTATTCAGTAAAGAAGCTGAGGTGCACACTTCCCGAAAGGAAGAGAAGTTAAGAAGCCATCTGACGCATCTTGCCAAATTTAAAGGCAATGAGAAATCCAAGTTCAGGAAGGAGTCTTCTTGGACCAAGTTTTTCTATCACAGTGGAGGGAAGGTCACTGCCGTTGAAGCTGTAGATGAGTTGTCTGTTGACTTGTCCGAGTTGTATTTTGGGCCTAGATTTGCTCATGGAGCTCATAGTCGGCTTTACCATGGATTTTACTATGACAAAGTTGTTGCTGTTAAGATCATCAATCTACCGGAAGATGATGAAAATGGAGATCTTGCAGGTCGCTTAACCAAGCAGTTTGGTAGGGAAGTAACCCTTTTATCTCATCTTCACCATCCCAATGTTGTAAAGGTAAACAGCTCCCTCCTTGATTGATGACAAGTTTATCTGAATTACAGTTCTGAGGCTATTTTAACTAGTTTTTAGGCATATCTGTCTGCTGAACTTTGATGGCATAAGTTTCAACTGAGAGAAATATTATCCTTAACGTTTGATGTGAGTTCCCGGTTTCTGCTCGAAAAGAAAAAGAACCTGTCTAATGAGTTATGGATTGACAGTGTTGTATTCACATTGTCTTGCAGTTGGTAGCAGCTTGTAGAAAGCCACCTGCTTATTGGATCATCACTGAGTATCTATGTCAAGGTTCCTTAAGGGCATATCTACACAAACTTGAAAACAAATCTCTCCCACTGCAAAAACTGATTGCCATTGCCTTGGACATTGCTCGTGGGATGGAATACATTCACTCACAAGGCGTCATTCATCGGGATCTCAAACCTGAAAACGTTCTTATCGATCAAGACTTTTGTCTAAAGATTGCCGATTTCGGCATTGCTTGTGAGGAAGTACATTATGATACACTGGCCGACGACCCTGGAACATATCGTTGGATGGCCCCCGAGATGATCAAACGTAAACCTTATGGACGAAAGGTTGATCTCTACAGCTTCGGACTTATCCTGTGGGAATTGGTGGCTGGAAGAATCCCTTATGAGGATATGACTCCCATTCAAGCAGCTTATGCAGTTGTAAATAAGGTATTAGTTGTTTTATACCATCTTCATTTTTACTAACTTATGTTTTCACATCTGAAACACAGTGGCTGTTGTTGTTTTTTTTTTTCCCAGAATATTAGACCGGTCATCCCGAGCGAGTGTCCCTCGGTGATGCGGGCGTTGATCGAGAAATGTTGGTCGGTGCATCCGGAAAAGAGGCCGGAGTTTTGGCAGGTGGTGAAAGTACTAGAGCAACTTGAGAGTTCAGTTGGGGGAGATGGTACATTGATGAATGTAGAGCAGAAGCCAAGTTGGGAAGATCACAAGAAAGGTTTAAAGCGTTGGATACAAAAGCTTGGTCCTCATCATTCTCAGACTTACTCTAACTTCCACATCTAAATTCATATGATTCTTTCTCTGCCTTTTTCTTGAAAATATCACATTTGCTCCTCAGGTTCAATGTAGATAAAAACAAAAAACTTTTGCAGTTTTTTTGGTTCCTTCATTTGTTTTTGTTTCTAACATTCACGTGACTGAAATGAATACGGTACGTTTCTTAAGGAAAAAAAATATCAAAGGCTTGAAATTATTGTACTTCTCTCACAATCTGTAAACTTATCCAAAATTTAGTAGTGGTGCCTTGTTGTTTTCTTTGGAGTCATCAGTGTCAGTCCATTTGACAAGACCATTATCAGTCTAGCTCAACCAGTCAAATAATGTCCACAAACTTATATTATAAGTTTGATGTCAAGATTGTGTCCCAGG

mRNA sequence

GAGGGTTCGGGGGGAGACTGCGACTTGGGGGCTCCGAGATTGAGACTTTCTCTTGGTGTTTCATGGGATATATTGGTTTTCAAGGGATTTTGGTTTTGATGGATATGAGATTATTGGTTCAGACTATCTTGTTCTTGATTGATCTTCATGGCTTTGATTAGCCATCTCAAGGTTCTGCTAAAGAAGAACAAGTTCTGTTTCTTTGCTTGTTTTGCTTCCTGTTTTTCTTAATTGTTGATGGGATTTGTTGAATTGGGGTTCTGGAGCTGTATGATTCAGACTAGTTTGTCTCTGAACTACCCTTTTGAATTCAAGCCTTCTTCTCAGCTGGTGAAGAAGAACAAGAAGAAAAGTAAGAAGGTTTTTGGATACGAGATCGATTAGTTTCTTTTTGTTTTTCAGTCTTTAGAAAATCATGGAGGAAGATGCTAATTCTTGGTTAAGAAGGACCAACTTTTCTCATACAATATGCTACCGTTTAGAGTCCTTACGATTGGCTTCCTTTCCTGTCACCACTCAGCCACCACTCAATTCAGTTGCGCAATCAAAAGCTGTCCCAGTTCCTTCTCATCATAGATCTACCCTTTGTTATAACCTACCCATTGAGAACAATCATGTTCCTAACAAGCAGAAGTTGTCTGTGTCTCTGCCTCCACAAACTACACTCTCGAGTGCATTTAAGGATGCACGTAAGGATCAGAAGCGATTTTCAACTCCACAACCTCGGAGGAGAGCAACCAAAAAAGAGAAGGGCAAAAGGGTATTCAGTAAAGAAGCTGAGGTGCACACTTCCCGAAAGGAAGAGAAGTTAAGAAGCCATCTGACGCATCTTGCCAAATTTAAAGGCAATGAGAAATCCAAGTTCAGGAAGGAGTCTTCTTGGACCAAGTTTTTCTATCACAGTGGAGGGAAGGTCACTGCCGTTGAAGCTGTAGATGAGTTGTCTGTTGACTTGTCCGAGTTGTATTTTGGGCCTAGATTTGCTCATGGAGCTCATAGTCGGCTTTACCATGGATTTTACTATGACAAAGTTGTTGCTGTTAAGATCATCAATCTACCGGAAGATGATGAAAATGGAGATCTTGCAGGTCGCTTAACCAAGCAGTTTGGTAGGGAAGTAACCCTTTTATCTCATCTTCACCATCCCAATGTTGTAAAGTTGGTAGCAGCTTGTAGAAAGCCACCTGCTTATTGGATCATCACTGAGTATCTATGTCAAGGTTCCTTAAGGGCATATCTACACAAACTTGAAAACAAATCTCTCCCACTGCAAAAACTGATTGCCATTGCCTTGGACATTGCTCGTGGGATGGAATACATTCACTCACAAGGCGTCATTCATCGGGATCTCAAACCTGAAAACGTTCTTATCGATCAAGACTTTTGTCTAAAGATTGCCGATTTCGGCATTGCTTGTGAGGAAGTACATTATGATACACTGGCCGACGACCCTGGAACATATCGTTGGATGGCCCCCGAGATGATCAAACGTAAACCTTATGGACGAAAGGTTGATCTCTACAGCTTCGGACTTATCCTGTGGGAATTGGTGGCTGGAAGAATCCCTTATGAGGATATGACTCCCATTCAAGCAGCTTATGCAGTTGTAAATAAGAATATTAGACCGGTCATCCCGAGCGAGTGTCCCTCGGTGATGCGGGCGTTGATCGAGAAATGTTGGTCGGTGCATCCGGAAAAGAGGCCGGAGTTTTGGCAGGTGGTGAAAGTACTAGAGCAACTTGAGAGTTCAGTTGGGGGAGATGGTACATTGATGAATGTAGAGCAGAAGCCAAGTTGGGAAGATCACAAGAAAGGTTTAAAGCGTTGGATACAAAAGCTTGGTCCTCATCATTCTCAGACTTACTCTAACTTCCACATCTAAATTCATATGATTCTTTCTCTGCCTTTTTCTTGAAAATATCACATTTGCTCCTCAGGTTCAATGTAGATAAAAACAAAAAACTTTTGCAGTTTTTTTGGTTCCTTCATTTGTTTTTGTTTCTAACATTCACGTGACTGAAATGAATACGGTACGTTTCTTAAGGAAAAAAAATATCAAAGGCTTGAAATTATTGTACTTCTCTCACAATCTGTAAACTTATCCAAAATTTAGTAGTGGTGCCTTGTTGTTTTCTTTGGAGTCATCAGTGTCAGTCCATTTGACAAGACCATTATCAGTCTAGCTCAACCAGTCAAATAATGTCCACAAACTTATATTATAAGTTTGATGTCAAGATTGTGTCCCAGG

Coding sequence (CDS)

ATGGAGGAAGATGCTAATTCTTGGTTAAGAAGGACCAACTTTTCTCATACAATATGCTACCGTTTAGAGTCCTTACGATTGGCTTCCTTTCCTGTCACCACTCAGCCACCACTCAATTCAGTTGCGCAATCAAAAGCTGTCCCAGTTCCTTCTCATCATAGATCTACCCTTTGTTATAACCTACCCATTGAGAACAATCATGTTCCTAACAAGCAGAAGTTGTCTGTGTCTCTGCCTCCACAAACTACACTCTCGAGTGCATTTAAGGATGCACGTAAGGATCAGAAGCGATTTTCAACTCCACAACCTCGGAGGAGAGCAACCAAAAAAGAGAAGGGCAAAAGGGTATTCAGTAAAGAAGCTGAGGTGCACACTTCCCGAAAGGAAGAGAAGTTAAGAAGCCATCTGACGCATCTTGCCAAATTTAAAGGCAATGAGAAATCCAAGTTCAGGAAGGAGTCTTCTTGGACCAAGTTTTTCTATCACAGTGGAGGGAAGGTCACTGCCGTTGAAGCTGTAGATGAGTTGTCTGTTGACTTGTCCGAGTTGTATTTTGGGCCTAGATTTGCTCATGGAGCTCATAGTCGGCTTTACCATGGATTTTACTATGACAAAGTTGTTGCTGTTAAGATCATCAATCTACCGGAAGATGATGAAAATGGAGATCTTGCAGGTCGCTTAACCAAGCAGTTTGGTAGGGAAGTAACCCTTTTATCTCATCTTCACCATCCCAATGTTGTAAAGTTGGTAGCAGCTTGTAGAAAGCCACCTGCTTATTGGATCATCACTGAGTATCTATGTCAAGGTTCCTTAAGGGCATATCTACACAAACTTGAAAACAAATCTCTCCCACTGCAAAAACTGATTGCCATTGCCTTGGACATTGCTCGTGGGATGGAATACATTCACTCACAAGGCGTCATTCATCGGGATCTCAAACCTGAAAACGTTCTTATCGATCAAGACTTTTGTCTAAAGATTGCCGATTTCGGCATTGCTTGTGAGGAAGTACATTATGATACACTGGCCGACGACCCTGGAACATATCGTTGGATGGCCCCCGAGATGATCAAACGTAAACCTTATGGACGAAAGGTTGATCTCTACAGCTTCGGACTTATCCTGTGGGAATTGGTGGCTGGAAGAATCCCTTATGAGGATATGACTCCCATTCAAGCAGCTTATGCAGTTGTAAATAAGAATATTAGACCGGTCATCCCGAGCGAGTGTCCCTCGGTGATGCGGGCGTTGATCGAGAAATGTTGGTCGGTGCATCCGGAAAAGAGGCCGGAGTTTTGGCAGGTGGTGAAAGTACTAGAGCAACTTGAGAGTTCAGTTGGGGGAGATGGTACATTGATGAATGTAGAGCAGAAGCCAAGTTGGGAAGATCACAAGAAAGGTTTAAAGCGTTGGATACAAAAGCTTGGTCCTCATCATTCTCAGACTTACTCTAACTTCCACATCTAA

Protein sequence

MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYNLPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKEAEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDLSELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSHLHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGMEYIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRKPYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEKCWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQKPSWEDHKKGLKRWIQKLGPHHSQTYSNFHI
Homology
BLAST of Tan0008726 vs. ExPASy Swiss-Prot
Match: Q2MHE4 (Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=HT1 PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.7e-85
Identity = 159/342 (46.49%), Postives = 219/342 (64.04%), Query Frame = 0

Query: 134 SHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAV----DELSVDLSELYFGPRF 193
           S     A+ +  EK +F    SW+         V   EA     +E + DLS+L+ G +F
Sbjct: 35  SKTNRYAEAETMEKKRFDSMESWSMIL--ESENVETWEASKGEREEWTADLSQLFIGNKF 94

Query: 194 AHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSHLHHPNVVKL 253
           A GAHSR+Y G Y  + VAVK++ +P   E  +   +L +QF  EV LLS L HPN+V+ 
Sbjct: 95  ASGAHSRIYRGIYKQRAVAVKMVRIPTHKE--ETRAKLEQQFKSEVALLSRLFHPNIVQF 154

Query: 254 VAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGMEYIHSQGVIH 313
           +AAC+KPP Y IITEY+ QG+LR YL+K E  SL ++ ++ +ALDI+RGMEY+HSQGVIH
Sbjct: 155 IAACKKPPVYCIITEYMSQGNLRMYLNKKEPYSLSIETVLRLALDISRGMEYLHSQGVIH 214

Query: 314 RDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRKPYGRKVDLY 373
           RDLK  N+L++ +  +K+ADFG +C E        + GTYRWMAPEMIK KPY RKVD+Y
Sbjct: 215 RDLKSNNLLLNDEMRVKVADFGTSCLETQCREAKGNMGTYRWMAPEMIKEKPYTRKVDVY 274

Query: 374 SFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEKCWSVHPEKR 433
           SFG++LWEL    +P++ MTP+QAA+AV  KN RP +P+ C   +  LI++CWS +P KR
Sbjct: 275 SFGIVLWELTTALLPFQGMTPVQAAFAVAEKNERPPLPASCQPALAHLIKRCWSENPSKR 334

Query: 434 PEFWQVVKVLEQLESSVGGDGTLMN----VEQKPSWEDHKKG 468
           P+F  +V VLE+ +  V     L +     + K +  DH KG
Sbjct: 335 PDFSNIVAVLEKYDECVKEGLPLTSHASLTKTKKAILDHLKG 372

BLAST of Tan0008726 vs. ExPASy Swiss-Prot
Match: Q9ZQ31 (Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 PE=1 SV=2)

HSP 1 Score: 289.7 bits (740), Expect = 6.3e-77
Identity = 128/273 (46.89%), Postives = 196/273 (71.79%), Query Frame = 0

Query: 174 DELSVDLSELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGR 233
           DE ++DL +L  GP FA GA  +LY G Y  + VA+KI+  PE+  + + A  + +QF +
Sbjct: 121 DEWTIDLRKLNMGPAFAQGAFGKLYKGTYNGEDVAIKILERPEN--SPEKAQFMEQQFQQ 180

Query: 234 EVTLLSHLHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIAL 293
           EV++L++L HPN+V+ + ACRKP  + I+TEY   GS+R +L + +N+++PL+  +  AL
Sbjct: 181 EVSMLANLKHPNIVRFIGACRKPMVWCIVTEYAKGGSVRQFLTRRQNRAVPLKLAVKQAL 240

Query: 294 DIARGMEYIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMA 353
           D+ARGM Y+H +  IHRDLK +N+LI  D  +KIADFG+A  EV  + +  + GTYRWMA
Sbjct: 241 DVARGMAYVHGRNFIHRDLKSDNLLISADKSIKIADFGVARIEVQTEGMTPETGTYRWMA 300

Query: 354 PEMIKRKPYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSV 413
           PEMI+ + Y +KVD+YSFG++LWEL+ G +P+++MT +QAA+AVVN+ +RP +P++C  V
Sbjct: 301 PEMIQHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPNDCLPV 360

Query: 414 MRALIEKCWSVHPEKRPEFWQVVKVLEQLESSV 447
           +  ++ +CW  +PE RP F +VVK+LE  E+ +
Sbjct: 361 LSDIMTRCWDANPEVRPCFVEVVKLLEAAETEI 391

BLAST of Tan0008726 vs. ExPASy Swiss-Prot
Match: F4JTP5 (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.1e-63
Identity = 118/309 (38.19%), Postives = 185/309 (59.87%), Query Frame = 0

Query: 171 EAVDELSVDLSELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQ 230
           +  D   ++L  L FG + A G++  LY G Y  + VA+K++  PE      L   L K+
Sbjct: 278 DGTDVWEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLK-PE-----RLDSDLEKE 337

Query: 231 FGREVTLLSHLHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIA 290
           F +EV ++  + H NVV+ + AC KPP   I+TE++  GS+  YLHK +     L  L  
Sbjct: 338 FAQEVFIMRKVRHKNVVQFIGACTKPPHLCIVTEFMPGGSVYDYLHK-QKGVFKLPTLFK 397

Query: 291 IALDIARGMEYIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYR 350
           +A+DI +GM Y+H   +IHRDLK  N+L+D++  +K+ADFG+A  +     +  + GTYR
Sbjct: 398 VAIDICKGMSYLHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTGVMTAETGTYR 457

Query: 351 WMAPEMIKRKPYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSEC 410
           WMAPE+I+ KPY  K D++S+G++LWEL+ G++PYE MTP+QAA  VV K +RP IP   
Sbjct: 458 WMAPEVIEHKPYDHKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNT 517

Query: 411 PSVMRALIEKCWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQKPSWEDHKKGLKR 470
              +  L+E+ W     +RP+F ++++ L+++   VG +G     E+K S      G+  
Sbjct: 518 HPKLAELLERLWEHDSTQRPDFSEIIEQLQEIAKEVGEEGE----EKKKSSTGLGGGIFA 575

Query: 471 WIQKLGPHH 480
            +++   HH
Sbjct: 578 ALRRSTTHH 575

BLAST of Tan0008726 vs. ExPASy Swiss-Prot
Match: O22558 (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=1 SV=2)

HSP 1 Score: 241.1 bits (614), Expect = 2.6e-62
Identity = 106/276 (38.41%), Postives = 177/276 (64.13%), Query Frame = 0

Query: 171 EAVDELSVDLSELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQ 230
           +  DE  +D+++L    + A G++  L+ G Y  + VA+K +  P+   N      + ++
Sbjct: 274 DGTDEWEIDVTQLKIEKKVASGSYGDLHRGTYCSQEVAIKFLK-PDRVNN-----EMLRE 333

Query: 231 FGREVTLLSHLHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIA 290
           F +EV ++  + H NVV+ + AC + P   I+TE++ +GS+  +LHK +  +  LQ L+ 
Sbjct: 334 FSQEVFIMRKVRHKNVVQFLGACTRSPTLCIVTEFMARGSIYDFLHK-QKCAFKLQTLLK 393

Query: 291 IALDIARGMEYIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYR 350
           +ALD+A+GM Y+H   +IHRDLK  N+L+D+   +K+ADFG+A  ++    +  + GTYR
Sbjct: 394 VALDVAKGMSYLHQNNIIHRDLKTANLLMDEHGLVKVADFGVARVQIESGVMTAETGTYR 453

Query: 351 WMAPEMIKRKPYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSEC 410
           WMAPE+I+ KPY  K D++S+ ++LWEL+ G IPY  +TP+QAA  VV K +RP IP + 
Sbjct: 454 WMAPEVIEHKPYNHKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKT 513

Query: 411 PSVMRALIEKCWSVHPEKRPEFWQVVKVLEQLESSV 447
              ++ L+E+CW   PE+RP F +++++L+Q+   V
Sbjct: 514 HPKVKGLLERCWHQDPEQRPLFEEIIEMLQQIMKEV 542

BLAST of Tan0008726 vs. ExPASy Swiss-Prot
Match: Q8RWL6 (Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 5.8e-62
Identity = 107/279 (38.35%), Postives = 172/279 (61.65%), Query Frame = 0

Query: 171 EAVDELSVDLSELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQ 230
           +  DE  +D+ +L    + A G++  L+ G Y  + VA+KI+  PE      +   + ++
Sbjct: 280 DGTDEWEIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILK-PE-----RVNAEMLRE 339

Query: 231 FGREVTLLSHLHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIA 290
           F +EV ++  + H NVV+ + AC + P   I+TE++ +GS+  +LHK       +Q L+ 
Sbjct: 340 FSQEVYIMRKVRHKNVVQFIGACTRSPNLCIVTEFMTRGSIYDFLHK-HKGVFKIQSLLK 399

Query: 291 IALDIARGMEYIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYR 350
           +ALD+++GM Y+H   +IHRDLK  N+L+D+   +K+ADFG+A  +     +  + GTYR
Sbjct: 400 VALDVSKGMNYLHQNNIIHRDLKTANLLMDEHEVVKVADFGVARVQTESGVMTAETGTYR 459

Query: 351 WMAPEMIKRKPYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSEC 410
           WMAPE+I+ KPY  + D++S+ ++LWEL+ G +PY  +TP+QAA  VV K +RP IP E 
Sbjct: 460 WMAPEVIEHKPYDHRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPKIPKET 519

Query: 411 PSVMRALIEKCWSVHPEKRPEFWQVVKVLEQLESSVGGD 450
              +  L+EKCW   P  RP F +++++L QL   VG D
Sbjct: 520 HPKLTELLEKCWQQDPALRPNFAEIIEMLNQLIREVGDD 551

BLAST of Tan0008726 vs. NCBI nr
Match: XP_022139851.1 (serine/threonine-protein kinase HT1-like [Momordica charantia])

HSP 1 Score: 794.7 bits (2051), Expect = 4.6e-226
Identity = 397/484 (82.02%), Postives = 429/484 (88.64%), Query Frame = 0

Query: 3   EDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYNLP 62
           EDANSWLRRT FSHT+ YRL+SLRLAS P+T QPP+NSV Q+K V  PSHHRSTL YN  
Sbjct: 2   EDANSWLRRTKFSHTVYYRLDSLRLASIPLTIQPPINSVGQTKVVTAPSHHRSTLRYN-- 61

Query: 63  IENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKEAE 122
            E+N+  NKQ+ S+S  PQTTLS A KDAR DQKRF+TPQP+RR + KEKGKRVF KEA+
Sbjct: 62  -ESNNFLNKQR-SLSPSPQTTLSGALKDARTDQKRFATPQPQRRESIKEKGKRVFCKEAK 121

Query: 123 VHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDLSE 182
           VH+S KEEKL+S L  LA  KG+ KSKF KESSWTK F HSGG+VTAVEAVDELSVDLS+
Sbjct: 122 VHSSLKEEKLKSPLKQLASLKGSPKSKFMKESSWTKCFEHSGGRVTAVEAVDELSVDLSK 181

Query: 183 LYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSHLH 242
           L+ G RFA+GAHSRLYHG YYDKVVAVKIINLP+DDENGDL  RLTKQFGREVTLLS LH
Sbjct: 182 LFLGNRFAYGAHSRLYHGIYYDKVVAVKIINLPDDDENGDLTARLTKQFGREVTLLSRLH 241

Query: 243 HPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGMEYI 302
           HPNV+KLVAACRKPP Y IITEYL QGSLRAYLHKLENKSLPL+KL+AIALDIARGMEYI
Sbjct: 242 HPNVIKLVAACRKPPVYCIITEYLSQGSLRAYLHKLENKSLPLKKLLAIALDIARGMEYI 301

Query: 303 HSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRKPY 362
           HSQGVIHRDLKPENVLIDQDFCLKIADFGIACEE H DTLA+DPGTYRWMAPEMIKRKPY
Sbjct: 302 HSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEAHCDTLANDPGTYRWMAPEMIKRKPY 361

Query: 363 GRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEKCW 422
           GRKVDLYSFGLILWELVAG +PYEDMTPIQAAYAVVNKNIRP IP ECP V+RALIE+CW
Sbjct: 362 GRKVDLYSFGLILWELVAGTVPYEDMTPIQAAYAVVNKNIRPAIPRECPPVLRALIEQCW 421

Query: 423 SVHPEKRPEFWQVVKVLEQLESSV-GGDGTLMNVEQKPSWEDHKKGLKRWIQKLGPHHSQ 482
           S   EKRPEFWQVVKVLEQLESSV GGDGTLM+VEQ+PSWEDHKKGLK W+QKLGP HSQ
Sbjct: 422 SEQSEKRPEFWQVVKVLEQLESSVGGGDGTLMSVEQQPSWEDHKKGLKHWMQKLGPQHSQ 481

Query: 483 TYSN 486
           T S+
Sbjct: 482 TSSH 481

BLAST of Tan0008726 vs. NCBI nr
Match: KAG7012438.1 (Serine/threonine-protein kinase HT1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 744.2 bits (1920), Expect = 7.2e-211
Identity = 377/482 (78.22%), Postives = 403/482 (83.61%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           MEED NSWLRRT FSHTICYRLESLRL SFP+T                PSH +STL   
Sbjct: 1   MEEDPNSWLRRTKFSHTICYRLESLRLPSFPIT---------------APSHPKSTL--- 60

Query: 61  LPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKE 120
                    NKQ+ S+S P QTTL S FKDAR D KRFSTPQPRRR  ++EKGKRVF+KE
Sbjct: 61  ---------NKQR-SLSPPSQTTLLSTFKDARNDSKRFSTPQPRRREPREEKGKRVFNKE 120

Query: 121 AEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDL 180
           A+VH   KEEKLRS + HLA FKGNE+S F KE SWTK F H  GKVTAV+AVDE SVDL
Sbjct: 121 AKVHNFSKEEKLRSPVRHLASFKGNERSTFMKEGSWTKLFEHKRGKVTAVDAVDEFSVDL 180

Query: 181 SELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSH 240
           S+LYFG +FA+GAHS LYHG Y+DKVVA+K+INLP+ DENGDLAGR+TKQFGREV LLS 
Sbjct: 181 SKLYFGFKFAYGAHSCLYHGLYFDKVVAIKVINLPKHDENGDLAGRITKQFGREVILLSR 240

Query: 241 LHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300
           LHHPNV+KLVAACRK P YWIITEYL QGSLRAYLHKLENKSLPLQKLIAIALDIARGME
Sbjct: 241 LHHPNVIKLVAACRK-PGYWIITEYLSQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300

Query: 301 YIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRK 360
           YIHSQ +IHRDLKPENVLIDQDFCLKIADFGIACEE H DTLADD GTYRWMAPEMIKRK
Sbjct: 301 YIHSQRIIHRDLKPENVLIDQDFCLKIADFGIACEEAHCDTLADDLGTYRWMAPEMIKRK 360

Query: 361 PYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEK 420
            YGRKVDLYSFGLILWELVAGRIPYE+MTPIQAAYAVVNKNIRPV+PSECP VMR LIE+
Sbjct: 361 HYGRKVDLYSFGLILWELVAGRIPYENMTPIQAAYAVVNKNIRPVVPSECPPVMRGLIEQ 420

Query: 421 CWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQKPSWEDHKKGLKRWIQKLGPHHS 480
           C SV P  RPEFW+VVKVLEQ+ESSVGGDGTLMNVEQKPSWE HKKGLK WIQKLG HHS
Sbjct: 421 CLSVQPANRPEFWEVVKVLEQVESSVGGDGTLMNVEQKPSWEVHKKGLKHWIQKLGTHHS 453

Query: 481 QT 483
            T
Sbjct: 481 HT 453

BLAST of Tan0008726 vs. NCBI nr
Match: KAG6573273.1 (Serine/threonine/tyrosine-protein kinase HT1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 741.9 bits (1914), Expect = 3.6e-210
Identity = 376/482 (78.01%), Postives = 403/482 (83.61%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           MEED NSWLRRT FSHTICYRLESLRL SFP+T                PSH +STL   
Sbjct: 1   MEEDPNSWLRRTKFSHTICYRLESLRLPSFPIT---------------APSHPKSTL--- 60

Query: 61  LPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKE 120
                    NKQ+ S+S P QTTL S FKDAR D KRFSTPQP RR  ++EKGKRVF+KE
Sbjct: 61  ---------NKQR-SLSPPSQTTLLSTFKDARNDSKRFSTPQPCRREPREEKGKRVFNKE 120

Query: 121 AEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDL 180
           A+VH S +EEKLRS + HLA FKGNE+S F KE SWTK F H  GKVTAV+AVDE SVDL
Sbjct: 121 AKVHNSSEEEKLRSPVRHLASFKGNERSTFMKEGSWTKLFEHKRGKVTAVDAVDEFSVDL 180

Query: 181 SELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSH 240
           S+LYFG +FA+GAHS LYHG Y+DKVVA+K+INLP+ DENGDLAGR+TKQFGREV LLS 
Sbjct: 181 SKLYFGFKFAYGAHSCLYHGLYFDKVVAIKVINLPKHDENGDLAGRITKQFGREVILLSR 240

Query: 241 LHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300
           LHHPNV+KLVAACRK P YWIITEYL QGSLRAYLHKLENKSLPLQKLIAIALDIARGME
Sbjct: 241 LHHPNVIKLVAACRK-PGYWIITEYLSQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300

Query: 301 YIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRK 360
           YIHSQ +IHRDLKPENVLIDQDFCLKIADFGIACEE H DTLADD GTYRWMAPEMIKRK
Sbjct: 301 YIHSQRIIHRDLKPENVLIDQDFCLKIADFGIACEEAHCDTLADDLGTYRWMAPEMIKRK 360

Query: 361 PYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEK 420
            YGRKVDLYSFGLILWELVAGRIPYE+MTPIQAAYAVVNKNIRPV+PSECP VMR LIE+
Sbjct: 361 HYGRKVDLYSFGLILWELVAGRIPYENMTPIQAAYAVVNKNIRPVVPSECPPVMRGLIEQ 420

Query: 421 CWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQKPSWEDHKKGLKRWIQKLGPHHS 480
           C SV P  RPEFW+VVKVLEQ+ESSVGGDGTLMNVEQKPSWE HKKGLK WIQKLG HHS
Sbjct: 421 CLSVQPANRPEFWEVVKVLEQVESSVGGDGTLMNVEQKPSWEVHKKGLKHWIQKLGTHHS 453

Query: 481 QT 483
            T
Sbjct: 481 HT 453

BLAST of Tan0008726 vs. NCBI nr
Match: XP_022955187.1 (serine/threonine-protein kinase HT1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 738.8 bits (1906), Expect = 3.0e-209
Identity = 375/482 (77.80%), Postives = 402/482 (83.40%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           MEED NSWLRRT FSHTICYRLESLRL SFP+T                PSH +STL   
Sbjct: 1   MEEDPNSWLRRTKFSHTICYRLESLRLPSFPIT---------------APSHPKSTL--- 60

Query: 61  LPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKE 120
                    NKQ+ S+S P QTTL S FKDAR D KRFSTPQP RR  ++EKGKRVF+KE
Sbjct: 61  ---------NKQR-SLSPPSQTTLLSTFKDARNDSKRFSTPQPCRREPREEKGKRVFNKE 120

Query: 121 AEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDL 180
           A+VH S +EEKLRS + HLA FKGNE+S F KE SWTK F H  GKVTAV+AVDE SVDL
Sbjct: 121 AKVHNSSEEEKLRSPVRHLASFKGNERSTFMKEGSWTKLFEHKRGKVTAVDAVDEFSVDL 180

Query: 181 SELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSH 240
           S+LYFG +FA+GAHS LYHG Y+DKVVA+K+INLP+ DENGDLAGR+TKQFGREV LLS 
Sbjct: 181 SKLYFGFKFAYGAHSCLYHGLYFDKVVAIKVINLPKHDENGDLAGRITKQFGREVILLSR 240

Query: 241 LHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300
           LHHPNV+KLVAACRK P YWIITEYL QGSLRAYLHKLENKSLPLQKLIAIALDIARGME
Sbjct: 241 LHHPNVIKLVAACRK-PGYWIITEYLSQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300

Query: 301 YIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRK 360
           YIHSQ +IHRDLKPENVLIDQDFCLKIADFGIACEE H DTLADD GTYRWMAPEMIKRK
Sbjct: 301 YIHSQRIIHRDLKPENVLIDQDFCLKIADFGIACEEAHCDTLADDLGTYRWMAPEMIKRK 360

Query: 361 PYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEK 420
            YGRKVDLYSFGLILWELVAGRIPYE+MTPIQAAYAVVNKNIRPV+PSEC  VMR LIE+
Sbjct: 361 HYGRKVDLYSFGLILWELVAGRIPYENMTPIQAAYAVVNKNIRPVVPSECTPVMRGLIEQ 420

Query: 421 CWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQKPSWEDHKKGLKRWIQKLGPHHS 480
           C SV P  RPEFW+VVKVLEQ+ESSVGGDGTLMNVEQKPSWE HKKGLK WIQKLG HHS
Sbjct: 421 CLSVQPANRPEFWEVVKVLEQVESSVGGDGTLMNVEQKPSWEVHKKGLKHWIQKLGTHHS 453

Query: 481 QT 483
            T
Sbjct: 481 HT 453

BLAST of Tan0008726 vs. NCBI nr
Match: XP_023542656.1 (serine/threonine-protein kinase HT1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 736.5 bits (1900), Expect = 1.5e-208
Identity = 374/482 (77.59%), Postives = 402/482 (83.40%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           MEED NSWLRRT FSHTICYRLESLRL SFP+T+               PS  +STL   
Sbjct: 1   MEEDPNSWLRRTKFSHTICYRLESLRLPSFPITS---------------PSRPKSTL--- 60

Query: 61  LPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKE 120
                    NKQ+ S+S P QTTL S FKDAR D KRFSTPQP RR  ++EKGK VF+KE
Sbjct: 61  ---------NKQR-SLSPPSQTTLLSTFKDARNDSKRFSTPQPCRREPREEKGKSVFNKE 120

Query: 121 AEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDL 180
           A+VH S +EEKLRS + HLA FKGNE+S F KE SWTK F H  GKVTAV+AVDE SVDL
Sbjct: 121 AKVHNSSEEEKLRSPVRHLASFKGNERSTFMKEGSWTKLFEHKRGKVTAVDAVDEFSVDL 180

Query: 181 SELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSH 240
           S+LYFG +FA+GAHS LYHG Y+DKVVA+K+INLP+ DENGDLAGR+TKQFGREV LLS 
Sbjct: 181 SKLYFGFKFAYGAHSCLYHGLYFDKVVAIKVINLPKHDENGDLAGRITKQFGREVILLSR 240

Query: 241 LHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300
           LHHPNV+KLVAACRK P YWIITEYL QGSLRAYLHKLENKSLPLQKLIAIALDIARGME
Sbjct: 241 LHHPNVIKLVAACRK-PGYWIITEYLSQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300

Query: 301 YIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRK 360
           YIHSQ +IHRDLKPENVLIDQDFCLKIADFGIACEE H DTLADD GTYRWMAPEMIKRK
Sbjct: 301 YIHSQRIIHRDLKPENVLIDQDFCLKIADFGIACEEAHCDTLADDLGTYRWMAPEMIKRK 360

Query: 361 PYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEK 420
            YGRKVDLYSFGLILWELVAGRIPYE+MTPIQAAYAVVNKNIRPV+PSECP VMR LIE+
Sbjct: 361 HYGRKVDLYSFGLILWELVAGRIPYENMTPIQAAYAVVNKNIRPVVPSECPPVMRGLIEQ 420

Query: 421 CWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQKPSWEDHKKGLKRWIQKLGPHHS 480
           C SV P  RPEFW+VVKVLEQ+ESSVGGDGTLMNVEQKPSWE HKKGLK WIQKLG HHS
Sbjct: 421 CLSVQPANRPEFWEVVKVLEQVESSVGGDGTLMNVEQKPSWEVHKKGLKHWIQKLGTHHS 453

Query: 481 QT 483
            T
Sbjct: 481 HT 453

BLAST of Tan0008726 vs. ExPASy TrEMBL
Match: A0A6J1CDY2 (serine/threonine-protein kinase HT1-like OS=Momordica charantia OX=3673 GN=LOC111010663 PE=4 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.2e-226
Identity = 397/484 (82.02%), Postives = 429/484 (88.64%), Query Frame = 0

Query: 3   EDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYNLP 62
           EDANSWLRRT FSHT+ YRL+SLRLAS P+T QPP+NSV Q+K V  PSHHRSTL YN  
Sbjct: 2   EDANSWLRRTKFSHTVYYRLDSLRLASIPLTIQPPINSVGQTKVVTAPSHHRSTLRYN-- 61

Query: 63  IENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKEAE 122
            E+N+  NKQ+ S+S  PQTTLS A KDAR DQKRF+TPQP+RR + KEKGKRVF KEA+
Sbjct: 62  -ESNNFLNKQR-SLSPSPQTTLSGALKDARTDQKRFATPQPQRRESIKEKGKRVFCKEAK 121

Query: 123 VHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDLSE 182
           VH+S KEEKL+S L  LA  KG+ KSKF KESSWTK F HSGG+VTAVEAVDELSVDLS+
Sbjct: 122 VHSSLKEEKLKSPLKQLASLKGSPKSKFMKESSWTKCFEHSGGRVTAVEAVDELSVDLSK 181

Query: 183 LYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSHLH 242
           L+ G RFA+GAHSRLYHG YYDKVVAVKIINLP+DDENGDL  RLTKQFGREVTLLS LH
Sbjct: 182 LFLGNRFAYGAHSRLYHGIYYDKVVAVKIINLPDDDENGDLTARLTKQFGREVTLLSRLH 241

Query: 243 HPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGMEYI 302
           HPNV+KLVAACRKPP Y IITEYL QGSLRAYLHKLENKSLPL+KL+AIALDIARGMEYI
Sbjct: 242 HPNVIKLVAACRKPPVYCIITEYLSQGSLRAYLHKLENKSLPLKKLLAIALDIARGMEYI 301

Query: 303 HSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRKPY 362
           HSQGVIHRDLKPENVLIDQDFCLKIADFGIACEE H DTLA+DPGTYRWMAPEMIKRKPY
Sbjct: 302 HSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEAHCDTLANDPGTYRWMAPEMIKRKPY 361

Query: 363 GRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEKCW 422
           GRKVDLYSFGLILWELVAG +PYEDMTPIQAAYAVVNKNIRP IP ECP V+RALIE+CW
Sbjct: 362 GRKVDLYSFGLILWELVAGTVPYEDMTPIQAAYAVVNKNIRPAIPRECPPVLRALIEQCW 421

Query: 423 SVHPEKRPEFWQVVKVLEQLESSV-GGDGTLMNVEQKPSWEDHKKGLKRWIQKLGPHHSQ 482
           S   EKRPEFWQVVKVLEQLESSV GGDGTLM+VEQ+PSWEDHKKGLK W+QKLGP HSQ
Sbjct: 422 SEQSEKRPEFWQVVKVLEQLESSVGGGDGTLMSVEQQPSWEDHKKGLKHWMQKLGPQHSQ 481

Query: 483 TYSN 486
           T S+
Sbjct: 482 TSSH 481

BLAST of Tan0008726 vs. ExPASy TrEMBL
Match: A0A6J1GSW0 (serine/threonine-protein kinase HT1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457228 PE=4 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.5e-209
Identity = 375/482 (77.80%), Postives = 402/482 (83.40%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           MEED NSWLRRT FSHTICYRLESLRL SFP+T                PSH +STL   
Sbjct: 1   MEEDPNSWLRRTKFSHTICYRLESLRLPSFPIT---------------APSHPKSTL--- 60

Query: 61  LPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKE 120
                    NKQ+ S+S P QTTL S FKDAR D KRFSTPQP RR  ++EKGKRVF+KE
Sbjct: 61  ---------NKQR-SLSPPSQTTLLSTFKDARNDSKRFSTPQPCRREPREEKGKRVFNKE 120

Query: 121 AEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDL 180
           A+VH S +EEKLRS + HLA FKGNE+S F KE SWTK F H  GKVTAV+AVDE SVDL
Sbjct: 121 AKVHNSSEEEKLRSPVRHLASFKGNERSTFMKEGSWTKLFEHKRGKVTAVDAVDEFSVDL 180

Query: 181 SELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSH 240
           S+LYFG +FA+GAHS LYHG Y+DKVVA+K+INLP+ DENGDLAGR+TKQFGREV LLS 
Sbjct: 181 SKLYFGFKFAYGAHSCLYHGLYFDKVVAIKVINLPKHDENGDLAGRITKQFGREVILLSR 240

Query: 241 LHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300
           LHHPNV+KLVAACRK P YWIITEYL QGSLRAYLHKLENKSLPLQKLIAIALDIARGME
Sbjct: 241 LHHPNVIKLVAACRK-PGYWIITEYLSQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300

Query: 301 YIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRK 360
           YIHSQ +IHRDLKPENVLIDQDFCLKIADFGIACEE H DTLADD GTYRWMAPEMIKRK
Sbjct: 301 YIHSQRIIHRDLKPENVLIDQDFCLKIADFGIACEEAHCDTLADDLGTYRWMAPEMIKRK 360

Query: 361 PYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEK 420
            YGRKVDLYSFGLILWELVAGRIPYE+MTPIQAAYAVVNKNIRPV+PSEC  VMR LIE+
Sbjct: 361 HYGRKVDLYSFGLILWELVAGRIPYENMTPIQAAYAVVNKNIRPVVPSECTPVMRGLIEQ 420

Query: 421 CWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQKPSWEDHKKGLKRWIQKLGPHHS 480
           C SV P  RPEFW+VVKVLEQ+ESSVGGDGTLMNVEQKPSWE HKKGLK WIQKLG HHS
Sbjct: 421 CLSVQPANRPEFWEVVKVLEQVESSVGGDGTLMNVEQKPSWEVHKKGLKHWIQKLGTHHS 453

Query: 481 QT 483
            T
Sbjct: 481 HT 453

BLAST of Tan0008726 vs. ExPASy TrEMBL
Match: A0A6J1JVP2 (serine/threonine-protein kinase HT1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490123 PE=4 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 7.2e-209
Identity = 372/482 (77.18%), Postives = 401/482 (83.20%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           ME+D NSWLRRT FSHTICYRLESLRL+SFP+T                P HH+STL   
Sbjct: 1   MEDDPNSWLRRTKFSHTICYRLESLRLSSFPIT---------------APYHHKSTL--- 60

Query: 61  LPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKE 120
                    NKQ+ S+S P QTTL   FKDAR D KRFSTPQPRR+  +KEKGKRVF+KE
Sbjct: 61  ---------NKQR-SLSPPSQTTLLGTFKDARNDSKRFSTPQPRRQEPRKEKGKRVFNKE 120

Query: 121 AEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDL 180
           A+VH   KEEKLRS + HLA  KGNE+S F KE SWTK F H  GKVTAV+AVDE SVDL
Sbjct: 121 AKVHNFSKEEKLRSPVRHLASCKGNERSTFMKEGSWTKLFEHKRGKVTAVDAVDEFSVDL 180

Query: 181 SELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSH 240
           S+LYFG +FA+GAHS LYHG Y+D+VVA+K+INLP+ DENGDLAGR+TKQFGREV LLS 
Sbjct: 181 SKLYFGFKFAYGAHSCLYHGLYFDQVVAIKVINLPKHDENGDLAGRITKQFGREVILLSR 240

Query: 241 LHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300
           LHHPNV+KLVAACRK P YWIITEYL QGSLRAYLHKLENKSLPLQKLIAIALDIARGME
Sbjct: 241 LHHPNVIKLVAACRK-PGYWIITEYLSQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300

Query: 301 YIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRK 360
           YIHSQ +IHRDLKPENVLIDQDFCLKIADFGIACEE H DTLADD GTYRWMAPEMIKRK
Sbjct: 301 YIHSQRIIHRDLKPENVLIDQDFCLKIADFGIACEEAHCDTLADDLGTYRWMAPEMIKRK 360

Query: 361 PYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEK 420
            YGRKVDLYSFGLILWELVAGRIPYE+MTPIQAAYAVVNKNIRPV+PSECP VMR LIE+
Sbjct: 361 HYGRKVDLYSFGLILWELVAGRIPYENMTPIQAAYAVVNKNIRPVVPSECPPVMRGLIEQ 420

Query: 421 CWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQKPSWEDHKKGLKRWIQKLGPHHS 480
           C  V P  RPEFW+VVKVLEQLESSVGGDGTL+NVEQKPSWE HKKGLK WIQKLG HHS
Sbjct: 421 CLLVQPANRPEFWEVVKVLEQLESSVGGDGTLVNVEQKPSWEVHKKGLKHWIQKLGTHHS 453

Query: 481 QT 483
            T
Sbjct: 481 HT 453

BLAST of Tan0008726 vs. ExPASy TrEMBL
Match: A0A6J1GT30 (serine/threonine-protein kinase HT1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457228 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 9.8e-206
Identity = 375/504 (74.40%), Postives = 402/504 (79.76%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           MEED NSWLRRT FSHTICYRLESLRL SFP+T                PSH +STL   
Sbjct: 1   MEEDPNSWLRRTKFSHTICYRLESLRLPSFPIT---------------APSHPKSTL--- 60

Query: 61  LPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKE 120
                    NKQ+ S+S P QTTL S FKDAR D KRFSTPQP RR  ++EKGKRVF+KE
Sbjct: 61  ---------NKQR-SLSPPSQTTLLSTFKDARNDSKRFSTPQPCRREPREEKGKRVFNKE 120

Query: 121 AEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDL 180
           A+VH S +EEKLRS + HLA FKGNE+S F KE SWTK F H  GKVTAV+AVDE SVDL
Sbjct: 121 AKVHNSSEEEKLRSPVRHLASFKGNERSTFMKEGSWTKLFEHKRGKVTAVDAVDEFSVDL 180

Query: 181 SELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSH 240
           S+LYFG +FA+GAHS LYHG Y+DKVVA+K+INLP+ DENGDLAGR+TKQFGREV LLS 
Sbjct: 181 SKLYFGFKFAYGAHSCLYHGLYFDKVVAIKVINLPKHDENGDLAGRITKQFGREVILLSR 240

Query: 241 LHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300
           LHHPNV+KLVAACRK P YWIITEYL QGSLRAYLHKLENKSLPLQKLIAIALDIARGME
Sbjct: 241 LHHPNVIKLVAACRK-PGYWIITEYLSQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300

Query: 301 YIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRK 360
           YIHSQ +IHRDLKPENVLIDQDFCLKIADFGIACEE H DTLADD GTYRWMAPEMIKRK
Sbjct: 301 YIHSQRIIHRDLKPENVLIDQDFCLKIADFGIACEEAHCDTLADDLGTYRWMAPEMIKRK 360

Query: 361 PYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNK-------------------- 420
            YGRKVDLYSFGLILWELVAGRIPYE+MTPIQAAYAVVNK                    
Sbjct: 361 HYGRKVDLYSFGLILWELVAGRIPYENMTPIQAAYAVVNKVIVVLYHLVSSQLKHCSCCL 420

Query: 421 --NIRPVIPSECPSVMRALIEKCWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQK 480
             NIRPV+PSEC  VMR LIE+C SV P  RPEFW+VVKVLEQ+ESSVGGDGTLMNVEQK
Sbjct: 421 FQNIRPVVPSECTPVMRGLIEQCLSVQPANRPEFWEVVKVLEQVESSVGGDGTLMNVEQK 475

Query: 481 PSWEDHKKGLKRWIQKLGPHHSQT 483
           PSWE HKKGLK WIQKLG HHS T
Sbjct: 481 PSWEVHKKGLKHWIQKLGTHHSHT 475

BLAST of Tan0008726 vs. ExPASy TrEMBL
Match: A0A1S3B6U5 (serine/threonine-protein kinase HT1-like OS=Cucumis melo OX=3656 GN=LOC103486451 PE=4 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 2.2e-205
Identity = 372/488 (76.23%), Postives = 403/488 (82.58%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           MEED NSWLRRTNFSHTICYRLESL LASFPVTTQ P     QSK  P         CYN
Sbjct: 1   MEEDPNSWLRRTNFSHTICYRLESLSLASFPVTTQSPPKPAVQSKPNP---------CYN 60

Query: 61  LPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKE 120
           L         KQ+ S+S  PQT LSSAFKDAR DQKRFSTPQP+R+   KEK KR+FSK 
Sbjct: 61  L--------TKQR-SLSPSPQTKLSSAFKDARIDQKRFSTPQPQRKEPLKEKSKRLFSKR 120

Query: 121 AEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEAVDELSVDL 180
           A+V  S KEEKL+  L HL  FKGNEK KF K+SSW+K F H GGKVTAVEAVDELS+DL
Sbjct: 121 AKVQNSLKEEKLKRPLRHLVSFKGNEKLKF-KDSSWSKLFEHGGGKVTAVEAVDELSIDL 180

Query: 181 SELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSH 240
           S+L FG RFA GAHSRLYHG Y DKVVA K+INLP +DENGDLAGRL KQFGREVTLLS 
Sbjct: 181 SKLMFGHRFAFGAHSRLYHGIYEDKVVAAKMINLPANDENGDLAGRLVKQFGREVTLLSR 240

Query: 241 LHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGME 300
           LHHPNV+KLVAA +KPP Y IITEYL QGSLRAYLHKLE KSLPLQK IAIALDIARGME
Sbjct: 241 LHHPNVIKLVAAVKKPPVYCIITEYLPQGSLRAYLHKLEKKSLPLQKQIAIALDIARGME 300

Query: 301 YIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRK 360
           YIHSQGVIHRDLKPEN+LID++FCLKIADFGIACEE H DTLA+DPGT+RWMAPEMIKRK
Sbjct: 301 YIHSQGVIHRDLKPENILIDENFCLKIADFGIACEEAHCDTLAEDPGTFRWMAPEMIKRK 360

Query: 361 PYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEK 420
           PYGRKVD+YSFGLILWELVAG+IPYEDMTPIQAA+AVV+KNIRPVIPSECPSV+RALIE+
Sbjct: 361 PYGRKVDIYSFGLILWELVAGKIPYEDMTPIQAAFAVVDKNIRPVIPSECPSVIRALIEQ 420

Query: 421 CWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLM---NVEQKPSWEDHKKGLKRWIQKLGP 480
           CW   PEKR EFWQVVKVLEQ+ES +GGDGTLM    ++ K SWEDHKKGLK WIQKLGP
Sbjct: 421 CWCEKPEKRLEFWQVVKVLEQIESCIGGDGTLMMSVELQGKASWEDHKKGLKHWIQKLGP 469

Query: 481 HHSQTYSN 486
            +S    N
Sbjct: 481 LNSHNSLN 469

BLAST of Tan0008726 vs. TAIR 10
Match: AT5G58950.1 (Protein kinase superfamily protein )

HSP 1 Score: 524.2 bits (1349), Expect = 1.1e-148
Identity = 272/507 (53.65%), Postives = 349/507 (68.84%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           M+E+A SW+RR  FS T+ YRL S +LAS P       +  +  KA+P  S   S+   +
Sbjct: 1   MDEEATSWIRRAKFSQTVSYRLNSSKLASLPFMINQ--DKFSGLKAIPQRSSSSSSASSS 60

Query: 61  LP---------------------------IENNHVPNKQKLSVSLPPQTTLSSAFKDARK 120
            P                           I+ N V +KQ+ SVS  PQ  +   FK+AR 
Sbjct: 61  DPKLVSSNSQTTGDTSSLEAADVYVVDSEIQTNPVTHKQR-SVSPSPQMAVPDVFKEARS 120

Query: 121 DQKRFSTPQPRRRATKKEKGKRVFSKEAEVHTSRKEEKLRSHLTHLAKFKGNEKSKFRKE 180
           ++KRFSTP PRR  ++K    ++  K +    S         +  L   +  E+ K +K+
Sbjct: 121 ERKRFSTPHPRRVESEKGMKPKLSHKNSFDKRSFNLRSPSGPIRDLGTLRIQERVKSKKD 180

Query: 181 SSWTKFFYHSGGKVTAVEAVDELSVDLSELYFGPRFAHGAHSRLYHGFYYDKVVAVKIIN 240
           + W+K F ++G +V+AVEA +E  VD+S+L+FG +FAHG +SRLYHG Y DK VAVK+I 
Sbjct: 181 TGWSKLFDNTGRRVSAVEASEEFRVDMSKLFFGLKFAHGLYSRLYHGKYEDKAVAVKLIT 240

Query: 241 LPEDDENGDLAGRLTKQFGREVTLLSHLHHPNVVKLVAACRKPPAYWIITEYLCQGSLRA 300
           +P+DD+NG L  RL KQF +EVTLLS L HPNV+K V A + PP Y ++T+YL +GSLR+
Sbjct: 241 VPDDDDNGCLGARLEKQFTKEVTLLSRLTHPNVIKFVGAYKDPPVYCVLTQYLPEGSLRS 300

Query: 301 YLHKLENKSLPLQKLIAIALDIARGMEYIHSQGVIHRDLKPENVLIDQDFCLKIADFGIA 360
           +LHK EN+SLPL+KLI  A+DIARGMEYIHS+ +IHRDLKPENVLID++F LKIADFGIA
Sbjct: 301 FLHKPENRSLPLKKLIEFAIDIARGMEYIHSRRIIHRDLKPENVLIDEEFHLKIADFGIA 360

Query: 361 CEEVHYDTLADDPGTYRWMAPEMIKRKPYGRKVDLYSFGLILWELVAGRIPYEDMTPIQA 420
           CEE + D LADDPGTYRWMAPEMIKRKP+GRK D+YSFGL+LWE+VAG IPYEDM PIQA
Sbjct: 361 CEEEYCDMLADDPGTYRWMAPEMIKRKPHGRKADVYSFGLVLWEMVAGAIPYEDMNPIQA 420

Query: 421 AYAVVNKNIRPVIPSECPSVMRALIEKCWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLM 480
           A+AVV+KNIRP IP +CP  M+ALIE+CWSV P+KRPEFWQ+VKVLEQ   S+  +G L 
Sbjct: 421 AFAVVHKNIRPAIPGDCPVAMKALIEQCWSVAPDKRPEFWQIVKVLEQFAISLEREGNL- 480

BLAST of Tan0008726 vs. TAIR 10
Match: AT3G46930.1 (Protein kinase superfamily protein )

HSP 1 Score: 425.2 bits (1092), Expect = 6.9e-119
Identity = 244/488 (50.00%), Postives = 310/488 (63.52%), Query Frame = 0

Query: 1   MEEDANSWLRRTNFSHTICYRLESLRLASFPVTTQPPLNSVAQSKAVPVPSHHRSTLCYN 60
           M+ +  SW+RR NFSHT+CYR+ +  L S P T       V Q K               
Sbjct: 1   MDGEVTSWIRRANFSHTVCYRMITPSLESMPFT-------VNQEK--------------- 60

Query: 61  LPIENNHVPNKQKLSVSLPPQTTLSSAFKDARKDQKRFSTPQPRRRATKKEKGKRVFSKE 120
             ++ N V NK K SVS  P   LS AF +A+ D KRFSTP PRR   + EKG +  S  
Sbjct: 61  --MQRNPVTNK-KRSVSPLPHMALSDAFIEAKSDIKRFSTPHPRR--VEPEKGMKAKSSS 120

Query: 121 AEVHTSRKEEKLRSHLTH--------LAKFKGNEKSKFRKESSWTKFFYHSGGKVTAVEA 180
            +  + +K   LRS L+H          K K   KSK  K+SS  K   + G KV++   
Sbjct: 121 RKDSSEKKSVNLRS-LSHSGPIRDLSTQKVKERGKSKIDKKSS--KSVDYRGSKVSSAGV 180

Query: 181 VDELSVDLSELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFG 240
           ++E  +D+S+L +G RFAHG +S++YHG Y  K VA+KII  PED ++  L  RL K+F 
Sbjct: 181 LEECLIDVSKLSYGDRFAHGKYSQIYHGEYEGKAVALKIITAPEDSDDIFLGARLEKEFI 240

Query: 241 REVTLLSHLHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIA 300
            E TLLS L HPNVVK V          IITEY+ +GSLR+YLHKLE KSLPL++LI   
Sbjct: 241 VEATLLSRLSHPNVVKFVGV---NTGNCIITEYVPRGSLRSYLHKLEQKSLPLEQLIDFG 300

Query: 301 LDIARGMEYIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWM 360
           LDIA+GMEYIHS+ ++H+DLKPENVLID DF LKIADFGIACEE + D L D+ GTYRWM
Sbjct: 301 LDIAKGMEYIHSREIVHQDLKPENVLIDNDFHLKIADFGIACEEEYCDVLGDNIGTYRWM 360

Query: 361 APEMIKRKPYGRKVDLYSFGLILWELVAGRIPYEDMT-PIQAAYAVVNKNIRPVIPSECP 420
           APE++KR P+GRK D+YSFGL+LWE+VAG +PYE+M    Q AYAV+ K IRPVIP++CP
Sbjct: 361 APEVLKRIPHGRKCDVYSFGLLLWEMVAGALPYEEMKFAEQIAYAVIYKKIRPVIPTDCP 420

Query: 421 SVMRALIEKCWSVHPEKRPEFWQVVKVLEQLESSVGGDGTLMNVEQKPSWEDHKKGLKRW 480
           + M+ LIE+CWS   +KRPEFWQ+VKVLE  + S+  +G L N+       + KK  K W
Sbjct: 421 AAMKELIERCWSSQTDKRPEFWQIVKVLEHFKKSLTSEGKL-NLLPSQICPELKKCPKFW 454

BLAST of Tan0008726 vs. TAIR 10
Match: AT1G62400.1 (Protein kinase superfamily protein )

HSP 1 Score: 318.5 bits (815), Expect = 9.1e-87
Identity = 157/330 (47.58%), Postives = 215/330 (65.15%), Query Frame = 0

Query: 146 EKSKFRKESSWTKFFYHSGGKVTAVEAV----DELSVDLSELYFGPRFAHGAHSRLYHGF 205
           EK +F    SW+         V   EA     +E + DLS+L+ G +FA GAHSR+Y G 
Sbjct: 2   EKKRFDSMESWSMIL--ESENVETWEASKGEREEWTADLSQLFIGNKFASGAHSRIYRGI 61

Query: 206 YYDKVVAVKIINLPEDDENGDLAGRLTKQFGREVTLLSHLHHPNVVKLVAACRKPPAYWI 265
           Y  + VAVK++ +P   E  +   +L +QF  EV LLS L HPN+V+ +AAC+KPP Y I
Sbjct: 62  YKQRAVAVKMVRIPTHKE--ETRAKLEQQFKSEVALLSRLFHPNIVQFIAACKKPPVYCI 121

Query: 266 ITEYLCQGSLRAYLHKLENKSLPLQKLIAIALDIARGMEYIHSQGVIHRDLKPENVLIDQ 325
           ITEY+ QG+LR YL+K E  SL ++ ++ +ALDI+RGMEY+HSQGVIHRDLK  N+L++ 
Sbjct: 122 ITEYMSQGNLRMYLNKKEPYSLSIETVLRLALDISRGMEYLHSQGVIHRDLKSNNLLLND 181

Query: 326 DFCLKIADFGIACEEVHYDTLADDPGTYRWMAPEMIKRKPYGRKVDLYSFGLILWELVAG 385
           +  +K+ADFG +C E        + GTYRWMAPEMIK KPY RKVD+YSFG++LWEL   
Sbjct: 182 EMRVKVADFGTSCLETQCREAKGNMGTYRWMAPEMIKEKPYTRKVDVYSFGIVLWELTTA 241

Query: 386 RIPYEDMTPIQAAYAVVNKNIRPVIPSECPSVMRALIEKCWSVHPEKRPEFWQVVKVLEQ 445
            +P++ MTP+QAA+AV  KN RP +P+ C   +  LI++CWS +P KRP+F  +V VLE+
Sbjct: 242 LLPFQGMTPVQAAFAVAEKNERPPLPASCQPALAHLIKRCWSENPSKRPDFSNIVAVLEK 301

Query: 446 LESSVGGDGTLMN----VEQKPSWEDHKKG 468
            +  V     L +     + K +  DH KG
Sbjct: 302 YDECVKEGLPLTSHASLTKTKKAILDHLKG 327

BLAST of Tan0008726 vs. TAIR 10
Match: AT2G24360.1 (Protein kinase superfamily protein )

HSP 1 Score: 289.7 bits (740), Expect = 4.5e-78
Identity = 128/273 (46.89%), Postives = 196/273 (71.79%), Query Frame = 0

Query: 174 DELSVDLSELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGR 233
           DE ++DL +L  GP FA GA  +LY G Y  + VA+KI+  PE+  + + A  + +QF +
Sbjct: 121 DEWTIDLRKLNMGPAFAQGAFGKLYKGTYNGEDVAIKILERPEN--SPEKAQFMEQQFQQ 180

Query: 234 EVTLLSHLHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIAL 293
           EV++L++L HPN+V+ + ACRKP  + I+TEY   GS+R +L + +N+++PL+  +  AL
Sbjct: 181 EVSMLANLKHPNIVRFIGACRKPMVWCIVTEYAKGGSVRQFLTRRQNRAVPLKLAVKQAL 240

Query: 294 DIARGMEYIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMA 353
           D+ARGM Y+H +  IHRDLK +N+LI  D  +KIADFG+A  EV  + +  + GTYRWMA
Sbjct: 241 DVARGMAYVHGRNFIHRDLKSDNLLISADKSIKIADFGVARIEVQTEGMTPETGTYRWMA 300

Query: 354 PEMIKRKPYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSV 413
           PEMI+ + Y +KVD+YSFG++LWEL+ G +P+++MT +QAA+AVVN+ +RP +P++C  V
Sbjct: 301 PEMIQHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPNDCLPV 360

Query: 414 MRALIEKCWSVHPEKRPEFWQVVKVLEQLESSV 447
           +  ++ +CW  +PE RP F +VVK+LE  E+ +
Sbjct: 361 LSDIMTRCWDANPEVRPCFVEVVKLLEAAETEI 391

BLAST of Tan0008726 vs. TAIR 10
Match: AT4G31170.1 (Protein kinase superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.7e-77
Identity = 127/273 (46.52%), Postives = 192/273 (70.33%), Query Frame = 0

Query: 174 DELSVDLSELYFGPRFAHGAHSRLYHGFYYDKVVAVKIINLPEDDENGDLAGRLTKQFGR 233
           +E ++DL +L+ GP FA GA  +LY G Y  + VA+K+  L   D N + A  L +QF +
Sbjct: 122 EEWTIDLRKLHMGPAFAQGAFGKLYRGTYNGEDVAIKL--LERSDSNPEKAQALEQQFQQ 181

Query: 234 EVTLLSHLHHPNVVKLVAACRKPPAYWIITEYLCQGSLRAYLHKLENKSLPLQKLIAIAL 293
           EV++L+ L HPN+V+ + AC KP  + I+TEY   GS+R +L K +N+++PL+  +  AL
Sbjct: 182 EVSMLAFLKHPNIVRFIGACIKPMVWCIVTEYAKGGSVRQFLTKRQNRAVPLKLAVMQAL 241

Query: 294 DIARGMEYIHSQGVIHRDLKPENVLIDQDFCLKIADFGIACEEVHYDTLADDPGTYRWMA 353
           D+ARGM Y+H +  IHRDLK +N+LI  D  +KIADFG+A  EV  + +  + GTYRWMA
Sbjct: 242 DVARGMAYVHERNFIHRDLKSDNLLISADRSIKIADFGVARIEVQTEGMTPETGTYRWMA 301

Query: 354 PEMIKRKPYGRKVDLYSFGLILWELVAGRIPYEDMTPIQAAYAVVNKNIRPVIPSECPSV 413
           PEMI+ +PY +KVD+YSFG++LWEL+ G +P+++MT +QAA+AVVN+ +RP +P++C  V
Sbjct: 302 PEMIQHRPYTQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPADCLPV 361

Query: 414 MRALIEKCWSVHPEKRPEFWQVVKVLEQLESSV 447
           +  ++ +CW   PE RP F ++V +LE  E+ +
Sbjct: 362 LGEIMTRCWDADPEVRPCFAEIVNLLEAAETEI 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2MHE41.7e-8546.49Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9ZQ316.3e-7746.89Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 P... [more]
F4JTP53.1e-6338.19Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 P... [more]
O225582.6e-6238.41Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=... [more]
Q8RWL65.8e-6238.35Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 P... [more]
Match NameE-valueIdentityDescription
XP_022139851.14.6e-22682.02serine/threonine-protein kinase HT1-like [Momordica charantia][more]
KAG7012438.17.2e-21178.22Serine/threonine-protein kinase HT1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6573273.13.6e-21078.01Serine/threonine/tyrosine-protein kinase HT1, partial [Cucurbita argyrosperma su... [more]
XP_022955187.13.0e-20977.80serine/threonine-protein kinase HT1-like isoform X2 [Cucurbita moschata][more]
XP_023542656.11.5e-20877.59serine/threonine-protein kinase HT1-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1CDY22.2e-22682.02serine/threonine-protein kinase HT1-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1GSW01.5e-20977.80serine/threonine-protein kinase HT1-like isoform X2 OS=Cucurbita moschata OX=366... [more]
A0A6J1JVP27.2e-20977.18serine/threonine-protein kinase HT1-like isoform X2 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1GT309.8e-20674.40serine/threonine-protein kinase HT1-like isoform X1 OS=Cucurbita moschata OX=366... [more]
A0A1S3B6U52.2e-20576.23serine/threonine-protein kinase HT1-like OS=Cucumis melo OX=3656 GN=LOC103486451... [more]
Match NameE-valueIdentityDescription
AT5G58950.11.1e-14853.65Protein kinase superfamily protein [more]
AT3G46930.16.9e-11950.00Protein kinase superfamily protein [more]
AT1G62400.19.1e-8747.58Protein kinase superfamily protein [more]
AT2G24360.14.5e-7846.89Protein kinase superfamily protein [more]
AT4G31170.11.7e-7746.52Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 347..357
score: 43.17
coord: 410..432
score: 37.46
coord: 301..319
score: 43.46
coord: 366..388
score: 28.13
coord: 263..276
score: 41.53
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 189..439
e-value: 3.1E-61
score: 206.9
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 183..442
e-value: 2.6E-58
score: 209.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 183..442
score: 42.40696
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 268..481
e-value: 3.0E-62
score: 211.9
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 171..267
e-value: 3.2E-18
score: 67.7
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 207..389
e-value: 1.0E-22
score: 78.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..132
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 113..132
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..106
NoneNo IPR availablePANTHERPTHR44329:SF192TYROSINE KINASE FAMILY PROTEINcoord: 3..463
NoneNo IPR availablePANTHERPTHR44329SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATEDcoord: 3..463
NoneNo IPR availableCDDcd13999STKc_MAP3K-likecoord: 190..439
e-value: 1.38893E-113
score: 333.35
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 307..319
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 161..439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0008726.1Tan0008726.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity