Tan0008725 (gene) Snake gourd v1

BLAST of Tan0008725 vs. ExPASy Swiss-Prot
Match: O23225 (U-box domain-containing protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUB5 PE=3 SV=3)

HSP 1 Score: 382.9 bits (982), Expect = 8.4e-105
Identity = 266/748 (35.56%), Postives = 399/748 (53.34%), Query Frame = 0

Query: 17  SFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLNIGKNKAELLLQYCRDS 76
           S+K+H  MC EL +LVDR+  I P+IE ARPG   G + LC L+   +K + LLQYC +S
Sbjct: 12  SYKMHSSMCLELKRLVDRIMRIFPDIEDARPGCSSGIQTLCLLHNALDKTKQLLQYCSES 71

Query: 77  SKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQIADDLRVAKFILDSSE 136
           SKLY+ +TGD I++R  R +  LE  L  I+ +VP  L  KISQI  DLR  +  L+ SE
Sbjct: 72  SKLYMAVTGDAILARGSRAKKSLEQCLNDIRSIVPTILEIKISQIVQDLRSTQLTLEFSE 131

Query: 137 EEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEVLFEKRSLRKLLDDVGH 196
           EE  KA+R+L++   S   +    EIK    AAL+L +S+ + ++ E+RSL+ + +D   
Sbjct: 132 EEAGKAIRELMQ--KSTSSSASPDEIKDFHYAALKLQLSTPEAIVTERRSLKIICED--- 191

Query: 197 GDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIEVNVRQRNNASQADIIL 256
               K+   T+                      I  S      E    + +N +      
Sbjct: 192 ---HKQNSFTH-------------------HQSIDDSLHANAAEAEASEEHNGTL----- 251

Query: 257 NRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHDTCPQTKMKLTDFSMTP 316
                PE+FKC +S  +MYDPV+I+S  T+E++ I+KWF+EG+D+CP +K KL DF++ P
Sbjct: 252 -----PEKFKCTLSRTVMYDPVIISSGNTFERMQIQKWFDEGNDSCPISKRKLDDFTLKP 311

Query: 317 NVDMKNLIDKWCVKFGVTIPDPS---LEPECPEVWENSIASFGSSMNDIRLPIDFSNISF 376
           NV++K+ I +WC K G+ + DP+   ++      +  SIASFGSS+ +I    D S IS 
Sbjct: 312 NVELKSQISEWCAKNGLDVQDPARKHVKASNSIDFSVSIASFGSSLYNIP---DHSGISI 371

Query: 377 GGLDNSYYPDSLRLNG-GNGLAIKYGQSKDDDLHRYQSDSNAEETNLEFPSTISELSWEL 436
              ++SY  DS   +    G      Q  D       +DS+  E  ++    ++ L W+ 
Sbjct: 372 TDFNSSYSIDSSSYSKMSKGGYFTPMQRIDSASGAGDTDSSHSEIEIDPLCGLTNLPWDA 431

Query: 437 KCKVIKDMKNAL--NKNGVGPILSETVMDQLALFLKDACDQQDS--EAQKNGSELFLSLV 496
           + KV++D+++    +      +     ++ L  +LK+A ++  +  E  K G +L L+ +
Sbjct: 432 QIKVVEDVRSRFEHSTRAFRSMSPSKFLEPLITYLKNALERNGTAGEIIKGGLDLLLAFL 491

Query: 497 RKNRSNSLNVPEKILTTLASLLNSE-VTYEVLAILEAFSGHRKCSSNFVTSGVLASMVEY 556
             NR    ++ E++    +  L SE V  E L ILE  S H    S   +SG L+S+++ 
Sbjct: 492 SGNRRAIESLEEEVFKMFSVFLESEVVAEEALNILEVLSNHPHGPSKITSSGSLSSLLKI 551

Query: 557 LDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDEALWGKCIFILKNLCH 616
           ++S+ + LQE A+ T  NLSS+  IC ++VSL  I KL   L  +      I ILKNLC 
Sbjct: 552 VESQAEHLQEQAMITLKNLSSSMEICLEMVSLDFIQKLTSFLQQKVFCKHSIIILKNLCS 611

Query: 617 TEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVMAEG--VFP 676
           TE+ R  I ET  C+ASI +LLE    E+QE+A++ILL LC Q++EYC LV+ E   ++ 
Sbjct: 612 TEKGRGCITETPDCLASIAELLESNVPEEQENAISILLQLCVQKIEYCCLVVREATDIYS 671

Query: 677 LLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQ-------- 736
            L  ISNNG+E+ K  A EL R L +V  ++++E  VS        AS + Q        
Sbjct: 672 SLILISNNGTEEVKVSASELLRALVEVDSDKEEEEEVSSRPEGRTTASPTSQVVTPVTHP 716

Query: 737 -----RKPSKKSGFLGRIFTKHSPLKKK 741
                    KKSG  G  F   S LKKK
Sbjct: 732 EPVKITPSPKKSGLFGFNF---SSLKKK 716

BLAST of Tan0008725 vs. ExPASy Swiss-Prot
Match: Q9CAG5 (U-box domain-containing protein 7 OS=Arabidopsis thaliana OX=3702 GN=PUB7 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 5.8e-82
Identity = 237/751 (31.56%), Postives = 391/751 (52.06%), Query Frame = 0

Query: 3   TDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLNIG 62
           T+  EN+ A S+    K+H  MC EL  ++ +V  I P +E ARP S  G +ALC+L+I 
Sbjct: 4   TELEENLFAASDA---KLHGDMCKELSGVLCKVLSIFPSLEGARPRSKSGIQALCSLHIA 63

Query: 63  KNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQIA 122
             KA+ +LQ+C + SKLYL +TGD ++ +  + +  L   L++++ +VP ++  +I +I 
Sbjct: 64  LEKAKNILQHCSECSKLYLAITGDAVLLKFEKAKIALIDGLKRVEDIVPSSIGSQILEIV 123

Query: 123 DDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEVLF 182
            +L   +F+LD SE+EV   +  LL+ G    +  +N+E++    AA RL+I+S +  L 
Sbjct: 124 GELENTRFMLDPSEKEVGDQIIALLQQGKKFDNCNDNTELEIFHRAATRLSITSSRVALA 183

Query: 183 EKRSLRKLLDDV-GHGDPPKKKILTYLLYLLKKHGELIVQEITE-----------ARADI 242
           E+R+L+KL+D      D  K+ I+ YLL+L++K  +L   EI +              D 
Sbjct: 184 ERRALKKLIDRARAEEDKRKESIVAYLLHLMRKCSKLFRSEILDENDSPGSYPCSPNEDH 243

Query: 243 SSSNGYGEIEVNVRQRNNASQADIILNR-AIPPEEFKCPISMRLMYDPVVIASKVTYEKV 302
            S +G+G         N+     I   +  +PPEE +CPIS++LM DPV+IAS  TYE+V
Sbjct: 244 GSVHGFGRQLSRFGSLNDKPMNSINSGQMPVPPEELRCPISLQLMCDPVIIASGQTYERV 303

Query: 303 WIEKWFEEGHDTCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWE 362
            IEKWF +GH+TCP+T+ +L   S+TPN  +K LI  WC + G  IP    E +  + W 
Sbjct: 304 CIEKWFSDGHNTCPKTQQQLPHISLTPNNCVKGLIASWCEQNGTQIPSGPPESQDLDYWR 363

Query: 363 NSIASFGSSMNDIRLPIDFSNISFGGLDNSYYPDSLRL--NGGNGLAIKYGQSKD----- 422
            +++   S+ +        S  S G    SY    +++     NG  +   Q+ +     
Sbjct: 364 LALSDSESTKSQ-------SVNSIG----SYKLKGVKIVPLEENGTTVVERQNTEESFVS 423

Query: 423 DDLHRYQSDSNAEETNLEFPSTIS-ELSWELKCKVIKDMKNALNKNGVGPIL--SETVMD 482
           DD     SD N  E   +  + ++ E   E KCKV++ ++  L  +    I   +   ++
Sbjct: 424 DDDDEEDSDINVLERYQDLLAVLNEEEGLEKKCKVVEKIRLLLKDDEEARIFMGANGFVE 483

Query: 483 QLALFLKDACDQQDSEAQKNGS-ELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTY-E 542
            L  FL  A D  ++ AQ +G+  LF   V  NR+  L +   ++  L  +++S  ++  
Sbjct: 484 ALLRFLGSAVDDNNAAAQDSGAMALFNLAVNNNRNKELMLTSGVIRLLEKMISSAESHGS 543

Query: 543 VLAILEAFSGHRKCSSNFVTSGVLASMVEYLDSEMKDLQEF-AIKTFSNLSSNSHICSDI 602
             A+    S   +  S   +S  +  +V+ L  E++   +  A+    NLS+ S     +
Sbjct: 544 ATALYLNLSCLDEAKSVIGSSQAVPFLVQLLQKEIETQCKLDALHALYNLSTYSPNIPAL 603

Query: 603 VSLGCIPKLVPLL--NDEALW-GKCIFILKNLCHTEEARIYIVETNGCIASIVQLLEMGS 662
           +S   I  L  LL    E LW  K + +L NL  ++E +   V + G I+S+  +L+MG 
Sbjct: 604 LSSNIIKSLQGLLASTGENLWIEKSLAVLLNLASSQEGKDEAVSSQGMISSLATVLDMGD 663

Query: 663 LEDQEHAVTILLSLCSQRVEYCELVMAEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQ 722
             +QE AV+ LL LC+ R    ++V+ EGV P L +IS NG+ +G+  + +L  L R+  
Sbjct: 664 TTEQEQAVSCLLILCNGRESCIQMVLQEGVIPSLVSISVNGTPRGREKSQKLLMLFRE-- 723

Query: 723 DNEQQESYVSDPSSSNKAASNSDQRKPSKKS 725
             E+Q+    D  SSN+      Q++P++KS
Sbjct: 724 --ERQQ---RDQPSSNR--DEPPQKEPARKS 731

BLAST of Tan0008725 vs. ExPASy Swiss-Prot
Match: O48700 (U-box domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUB6 PE=2 SV=2)

HSP 1 Score: 292.4 bits (747), Expect = 1.5e-77
Identity = 218/727 (29.99%), Postives = 363/727 (49.93%), Query Frame = 0

Query: 7   ENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLNIGKNKA 66
           EN+ A S+    K+H  MC EL  +  +V  I P +E ARP S  G + LC+L+I   KA
Sbjct: 8   ENLFAASDA---KLHGDMCKELSAVYCKVLSIFPSLEEARPRSKSGIQTLCSLHIALEKA 67

Query: 67  ELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQIADDLR 126
           + +LQ+C + SKLYL +TGD ++ +  + ++ L  +LR+++ +VP ++  +I  I  +L 
Sbjct: 68  KNILQHCSECSKLYLAITGDAVLLKFEKAKSALIDSLRRVEDIVPSSIGSQILDIVGELE 127

Query: 127 VAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEVLFEKRS 186
             KF+LD SE+EV   +  LL+ G    +  +++E++    AA RL+I+S +  L E+R+
Sbjct: 128 HTKFLLDPSEKEVGDRIIALLQQGKKFDNGSDSTELEIFHQAATRLSITSSRSALAERRA 187

Query: 187 LRKLLDDVG-HGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSS------NGYGEI 246
           L+K++D      D  K+ I+ YLL+L++K+ +L   E+ +      S+       G  E 
Sbjct: 188 LKKVIDRARVEEDKRKESIVAYLLHLMRKYSKLFRSEMMDENDSPCSTPCSPTGQGPNED 247

Query: 247 EVNVRQRNNASQADIIL---------NRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVW 306
            VN   R  +    I              IPPEE +CPIS++LMYDPV+IAS  TYE+V 
Sbjct: 248 RVNAFGRQLSKFGSINYKPMNSRKSGQMPIPPEELRCPISLQLMYDPVIIASGQTYERVC 307

Query: 307 IEKWFEEGHDTCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWEN 366
           IEKWF +GH++CP+T+ +L   S+TPN  +K LI  WC + G+T+P    E      W  
Sbjct: 308 IEKWFSDGHNSCPKTQQQLPHLSLTPNYCVKGLIASWCEQNGITVPTGPPESLDLNYWR- 367

Query: 367 SIASFGSSMNDIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGL-AIKYGQSKDDDLHRYQ 426
                  +M+D   P            NS   DS+ L     +  +   +S   +  R Q
Sbjct: 368 ------LAMSDSESP------------NSKSVDSVGLCTPKDIRVVPLEESSTIESERQQ 427

Query: 427 SDSNAEETNLEFPSTISELSWEL------------KCKVIKDMKNALNKNGVGPIL--SE 486
            + N     ++    + E   ++            KCKV+++++  L  N    IL  + 
Sbjct: 428 KEKNNAPDEVDSEINVLEGYQDILAIVDKEEDLAKKCKVVENVRILLKDNEEARILMGAN 487

Query: 487 TVMDQLALFLKDACDQQDSEAQKNGS-ELFLSLVRKNRSNSLNVPEKILTTLASLLN-SE 546
             ++    FL+ A    ++ AQ+ G+  LF   V  NR+  L +   ++  L  +++ S+
Sbjct: 488 GFVEAFLQFLESAVHDNNAAAQETGAMALFNLAVNNNRNKELMLTSGVIPLLEKMISCSQ 547

Query: 547 VTYEVLAILEAFSGHRKCSSNFVTSGVLASMVEYLDSEMKDLQEF-AIKTFSNLSSNSHI 606
                 A+    S   K      +S  ++  V  L  + K   +  A+    NLS+ S  
Sbjct: 548 SQGPATALYLNLSCLEKAKPVIGSSQAVSFFVNLLLQDTKTQCKLDALHALYNLSTYSPN 607

Query: 607 CSDIVSLGCIPKLVPLLN-DEALW-GKCIFILKNLCHTEEARIYIVETNGCIASIVQLLE 666
              ++S   I  L  L +    LW  K + +L NL  + E +  ++ T G I+++  +L+
Sbjct: 608 IPTLLSSNIIKSLQVLASTGNHLWIEKSLAVLLNLASSREGKEEMITTQGMISTLATVLD 667

Query: 667 MGSLEDQEHAVTILLSLCSQRVEYCELVMAEGVFPLLWNISNNGSEKGKAGAFELRRLLR 698
            G   +QE AV+ L+ LC+      ++V+ EGV P L +IS NGS +G+  + +L  L R
Sbjct: 668 TGDTVEQEQAVSCLVILCTGSESCIQMVLQEGVIPSLVSISVNGSPRGRDKSQKLLMLFR 712

BLAST of Tan0008725 vs. ExPASy Swiss-Prot
Match: Q9C7G1 (U-box domain-containing protein 45 OS=Arabidopsis thaliana OX=3702 GN=PUB45 PE=1 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 5.8e-74
Identity = 223/758 (29.42%), Postives = 382/758 (50.40%), Query Frame = 0

Query: 19  KVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLNIGKNKAELLLQYCRDSSK 78
           K+H +MC  L  +  ++  I P +EAARP S  G +ALC+L++   K + +L++C +SSK
Sbjct: 17  KLHGKMCNALSVIYCKIMSIFPSLEAARPRSKSGIQALCSLHVVLEKVKNILRHCTESSK 76

Query: 79  LYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQIADDLRVAKFILDSSEEE 138
           LYL +TGD ++ +  + ++ L  +LR+++ +V  ++  ++ +I  +L   +F LD +E+E
Sbjct: 77  LYLAITGDSVVLKFEKAKSSLTDSLRRVEDIVQQSIGSQLLEILMELENTEFSLDPAEKE 136

Query: 139 VWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEVLFEKRSLRKLLDDVG-HG 198
           +   +  LL+ G +   + +N+E++    AA RL I+S +  L E+R L+KL++      
Sbjct: 137 IGDQIIGLLQQGGNFESSSDNNELEVFHQAATRLGITSSRAALTERRCLKKLIERARMED 196

Query: 199 DPPKKKILTYLLYLLKKHGELIVQEITEARADISSS-------------NGYGEI----- 258
           D  K+ I+ YLL+L++K+ +L   EI +      SS             + +G       
Sbjct: 197 DKRKESIVAYLLHLMRKYSKLFRSEIWDDNDSQGSSSLPCSPTIQGSIDDAHGRAFDRQL 256

Query: 259 ----EVNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWF 318
                 N R  NN  ++      ++PPEE +CPIS++LMYDPV+IAS  TYE++ IEKWF
Sbjct: 257 SKLSSFNFRSCNNNRRSS---QMSVPPEELRCPISLQLMYDPVIIASGQTYERICIEKWF 316

Query: 319 EEGHDTCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASF 378
            +GH+TCP+T  +L+   +TPN  +K LI  WC + GV +PD   E      W   +A  
Sbjct: 317 SDGHNTCPKTHQQLSHLCLTPNYCVKALISSWCEQNGVQVPDGPPESLDLNYWR--LALS 376

Query: 379 GSSMNDIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAI-KYGQSKDDDLH-RYQSDS- 438
            S   D R              ++    S +L     + + + G  K++     YQ D  
Sbjct: 377 VSESTDTR--------------SAKRVGSCKLKDVKVVPLEESGTIKEEACESEYQEDQV 436

Query: 439 NAEETNLEFPSTISEL-SWELKCKVIKDMKNALNKNGVGPIL--SETVMDQLALFLKDAC 498
              E   E  +T++++ +   KC+V++ ++  L  +    IL      ++ L  FL  A 
Sbjct: 437 TLVERCTELLTTLTDVDTLRKKCRVVEQIRVLLKDDEEARILMGENGCVEALLQFLGSAL 496

Query: 499 DQQDSEAQKNGS-ELFLSLVRKNRSNSLNVPEKILTTLASLL-NSEVTYEVLAILEAFSG 558
           ++ ++ AQK G+  LF   V  NR+  L +   I+  L  +L N      V AI    S 
Sbjct: 497 NENNASAQKVGAMALFNLAVDNNRNKELMLASGIIPLLEEMLCNPHSHGSVTAIYLNLSC 556

Query: 559 HRKCSSNFVTSGVLASMVEYLDSEMK-DLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLV 618
             +      +S  +  MV  L +E +   +  A+ +  +LS+       ++S   +  L 
Sbjct: 557 LEEAKPVIGSSLAVPFMVNLLWTETEVQCKVDALHSLFHLSTYPPNIPCLLSADLVNALQ 616

Query: 619 PL-LNDEALW-GKCIFILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTIL 678
            L ++DE  W  K + +L NL   E  +  +V     ++++  +L+ G   +QE AV++L
Sbjct: 617 SLTISDEQRWTEKSLAVLLNLVLNEAGKDEMVSAPSLVSNLCTILDTGEPNEQEQAVSLL 676

Query: 679 LSLCSQRVEYCELVMAEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQ------Q 733
           L LC+      E+V+ EGV P L +IS NG+++G+  A +L  L R+++  +Q      Q
Sbjct: 677 LILCNHSEICSEMVLQEGVIPSLVSISVNGTQRGRERAQKLLTLFRELRQRDQTHLTEPQ 736

BLAST of Tan0008725 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 7.7e-34
Identity = 166/712 (23.31%), Postives = 317/712 (44.52%), Query Frame = 0

Query: 4   DGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEI-EAARPGSPEGREALCNLNIG 63
           D    + A+S+ Y   V  ++C  L + +  +  +  EI E+  P S +  + L NL   
Sbjct: 14  DVVNEIAAISD-YRITVK-KLCYNLARRLKLLVPMFEEIRESNEPISEDTLKTLMNLKEA 73

Query: 64  KNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYM---VPVALARKIS 123
              A+  L++C   SK+YL +  +++ S+   V   LE +L +I Y    +   +  ++ 
Sbjct: 74  MCSAKDYLKFCSQGSKIYLVMEREQVTSKLMEVSVKLEQSLSQIPYEELDISDEVREQVE 133

Query: 124 QIADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKE 183
            +    R AK  +D S++E+++ ++ L     S  DA +      L+  A +L++    +
Sbjct: 134 LVLSQFRRAKGRVDVSDDELYEDLQSLCN-KSSDVDAYQ----PVLERVAKKLHLMEIPD 193

Query: 184 VLFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEI 243
           +  E  +L +++   G GD  +   +  +  +LK     ++++  +   D    NG  E 
Sbjct: 194 LAQESVALHEMVASSG-GDVGEN--IEEMAMVLK-----MIKDFVQTEDD----NG-EEQ 253

Query: 244 EVNVRQRNNASQADIILNRAIP--PEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEE 303
           +V V  R+N  Q     ++ IP  P++F+CPIS+ +M DPV+++S  TYE+  IEKW E 
Sbjct: 254 KVGVNSRSN-GQTSTAASQKIPVIPDDFRCPISLEMMRDPVIVSSGQTYERTCIEKWIEG 313

Query: 304 GHDTCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDP--SLEPECPEVWENSIASF 363
           GH TCP+T+  LT  ++TPN  +++LI +WC    +  P P  SL P         ++SF
Sbjct: 314 GHSTCPKTQQALTSTTLTPNYVLRSLIAQWCEANDIEPPKPPSSLRP-------RKVSSF 373

Query: 364 GSSMNDIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAE 423
            S     ++      +++G       P+  R   G    I+    ++ D     +++ A 
Sbjct: 374 SSPAEANKIEDLMWRLAYGN------PEDQRSAAGE---IRLLAKRNADNRVAIAEAGAI 433

Query: 424 ETNLEFPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSE 483
              +   ST      E     + ++  ++ +N  G I+S   +  +   LK    +   E
Sbjct: 434 PLLVGLLSTPDSRIQEHSVTALLNL--SICENNKGAIVSAGAIPGIVQVLK----KGSME 493

Query: 484 AQKNGSELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNF 543
           A++N +             SL+V ++   T+ +L                          
Sbjct: 494 ARENAAATLF---------SLSVIDENKVTIGAL-------------------------- 553

Query: 544 VTSGVLASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLND--EA 603
              G +  +V  L+   +  ++ A     NL          +  G IP L  LL +    
Sbjct: 554 ---GAIPPLVVLLNEGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSG 613

Query: 604 LWGKCIFILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVE 663
           +  + + IL  L    E +  I+ ++  + S+V+ +  GS  ++E+A  +L+ LCS   +
Sbjct: 614 MVDEALAILAILSSHPEGKA-IIGSSDAVPSLVEFIRTGSPRNRENAAAVLVHLCSGDPQ 642

Query: 664 YCELVMAEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDP 706
           +       G+   L +++ NG+++GK  A +L   +  + + +Q+E+ VS P
Sbjct: 674 HLVEAQKLGLMGPLIDLAGNGTDRGKRKAAQLLERISRLAE-QQKETAVSQP 642

BLAST of Tan0008725 vs. NCBI nr
Match: XP_022942839.1 (U-box domain-containing protein 5-like [Cucurbita moschata])

HSP 1 Score: 1228.0 bits (3176), Expect = 0.0e+00
Identity = 631/735 (85.85%), Postives = 671/735 (91.29%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTD T+NVEAVSN+YSFKVHCRMCTELMKLVDRV EILPEIEAARPGSPEGREALCNLN
Sbjct: 1   MGTDSTKNVEAVSNIYSFKVHCRMCTELMKLVDRVLEILPEIEAARPGSPEGREALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
           IG  KAEL+LQYCRDSSKLYL LTGDRIISRCHRV++LLEHNLRKIKYMVPVALARKISQ
Sbjct: 61  IGMKKAELVLQYCRDSSKLYLALTGDRIISRCHRVKSLLEHNLRKIKYMVPVALARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           IADDL+VAKFILDSSEEEVWKAM+QLLKLG S PDA E SEIKALKIAALRLNI SYKE+
Sbjct: 121 IADDLKVAKFILDSSEEEVWKAMQQLLKLGPSSPDAPEISEIKALKIAALRLNILSYKEM 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           LFE+RS+RKLLDDVGHG+P K+KILTYL Y LKK+G+LI+QEI+EARADISSSNGYGE+E
Sbjct: 181 LFERRSIRKLLDDVGHGNPRKRKILTYLFYRLKKYGKLILQEISEARADISSSNGYGEME 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
            NVRQ N+  QAD+ILNRAIPPEEFKCPIS+RLMYDPVVI SKVTYEKVWIEKWF EGHD
Sbjct: 241 GNVRQGNDTPQADMILNRAIPPEEFKCPISLRLMYDPVVIESKVTYEKVWIEKWFNEGHD 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCP TKMKLT FSMTPNVDMK LI KWC+KF VTIPDPS+EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPLTKMKLTSFSMTPNVDMKKLIHKWCIKFRVTIPDPSVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           DIRLPIDFS+ISFGGL NSYYPDSLRL   N L IK  QSKDDDL R+QSDSNAEETNLE
Sbjct: 361 DIRLPIDFSDISFGGLHNSYYPDSLRL---NELGIKSRQSKDDDLGRFQSDSNAEETNLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPST+SELSWE K KVIKDM + +NKN  GPILSETVMDQLALF+KDA D QDSEAQKNG
Sbjct: 421 FPSTMSELSWESKSKVIKDMNDIINKNRFGPILSETVMDQLALFIKDAFDLQDSEAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
           SELFLSLVRK+RSNSL+VPEKILTTLASLLNSEVT EVLAILEA SGH KC S FVTSGV
Sbjct: 481 SELFLSLVRKSRSNSLSVPEKILTTLASLLNSEVTDEVLAILEALSGHHKCGSKFVTSGV 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDE-ALWGKCI 600
           LASMVEYLDSE+K LQEFA+KTF+NLSSNSHICSDIVSLGCIPKLV LLNDE  L GKC+
Sbjct: 541 LASMVEYLDSEIKALQEFAVKTFANLSSNSHICSDIVSLGCIPKLVSLLNDEDDLSGKCM 600

Query: 601 FILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVM 660
           FIL+NLCHTEE +IYIVETNGCIASIV+ LEMG LE+QEHA+TILLSLCSQRVEYCELVM
Sbjct: 601 FILQNLCHTEEGKIYIVETNGCIASIVRCLEMGKLENQEHAITILLSLCSQRVEYCELVM 660

Query: 661 AEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRK 720
            EGVFPLLW ISNNGSEKGKAGAFEL RLL+DVQDNEQQESYVSD SSSNK A NS QRK
Sbjct: 661 EEGVFPLLWKISNNGSEKGKAGAFELHRLLKDVQDNEQQESYVSDTSSSNKPACNSKQRK 720

Query: 721 PSKKSGFLGRIFTKH 735
            SKK GFLG IFTKH
Sbjct: 721 VSKKPGFLGMIFTKH 732

BLAST of Tan0008725 vs. NCBI nr
Match: KAG6600581.1 (U-box domain-containing protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1227.6 bits (3175), Expect = 0.0e+00
Identity = 631/735 (85.85%), Postives = 672/735 (91.43%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTD T+NVEAVSN+YSFKVHCRMCTELMKLVDRV EILPEIEAARPGSPEGREALCNLN
Sbjct: 1   MGTDSTKNVEAVSNIYSFKVHCRMCTELMKLVDRVLEILPEIEAARPGSPEGREALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
           IG  KAEL+LQYCRDSSKLYL LTGDRIISRCHRV++LLEHNLRKIKYMVPVALARKISQ
Sbjct: 61  IGMKKAELVLQYCRDSSKLYLALTGDRIISRCHRVKSLLEHNLRKIKYMVPVALARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           IADDL+VAKFILDSSEEEVWKAM+QLLKLG S PDA E SEIKALKIAALRLNI SYKE+
Sbjct: 121 IADDLKVAKFILDSSEEEVWKAMQQLLKLGASSPDAPEISEIKALKIAALRLNILSYKEM 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           LFE+RS+RKLLDDVGHG+P K+KILTYL Y LKK+G+LI+QEI+EARADISSSNGYGE+E
Sbjct: 181 LFERRSIRKLLDDVGHGNPRKRKILTYLFYRLKKYGKLILQEISEARADISSSNGYGEME 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
            NVRQ N+  QAD+ILNRAIPPEEFKCPIS+RLMYDPVVI SKVTYEKVWIEKWF+EGHD
Sbjct: 241 GNVRQGNDTPQADMILNRAIPPEEFKCPISLRLMYDPVVIESKVTYEKVWIEKWFKEGHD 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCP TKMKLT FSMTPNVDMK LI KWC+KF VTIPDPS+EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPLTKMKLTSFSMTPNVDMKKLIHKWCIKFRVTIPDPSVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           DIRLPIDFS+ISFGGL NSYYPDSLRL   N L+IK  QSK DDL R+QSDSNAEETNLE
Sbjct: 361 DIRLPIDFSDISFGGLHNSYYPDSLRL---NELSIKSRQSKGDDLGRFQSDSNAEETNLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPST+SELSWE K KVIKDM N +NKN  GPILSETVMDQLALF+KDA D QDSEAQKNG
Sbjct: 421 FPSTMSELSWESKSKVIKDMNNIINKNRFGPILSETVMDQLALFIKDAFDLQDSEAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
           SELFLSLVRK+RSNSL+VPEKILTTLASLLNSEVT EVLAILEA SGH KC S FVTSGV
Sbjct: 481 SELFLSLVRKSRSNSLSVPEKILTTLASLLNSEVTDEVLAILEALSGHHKCGSKFVTSGV 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDE-ALWGKCI 600
           LASMVEYLDSE+K LQEFA+KTF+NLSSNSHICSDIVSLGCIPKLV LLNDE  L GKC+
Sbjct: 541 LASMVEYLDSEIKALQEFAVKTFANLSSNSHICSDIVSLGCIPKLVSLLNDEDDLSGKCM 600

Query: 601 FILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVM 660
           FIL+NLCHTEE +IYIVETNGCIASIV+ LEMG LE+QEHA+TILLSLCSQRVEYCELVM
Sbjct: 601 FILQNLCHTEEGKIYIVETNGCIASIVRCLEMGKLENQEHAITILLSLCSQRVEYCELVM 660

Query: 661 AEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRK 720
            EGVFPLLW ISNNGSEKGKAGAFEL RLL+DVQDNEQQESYVSD SSSNK A NS QRK
Sbjct: 661 EEGVFPLLWKISNNGSEKGKAGAFELHRLLKDVQDNEQQESYVSDTSSSNKPACNSKQRK 720

Query: 721 PSKKSGFLGRIFTKH 735
            SKK GFLG IFTKH
Sbjct: 721 VSKKPGFLGMIFTKH 732

BLAST of Tan0008725 vs. NCBI nr
Match: KAG7031221.1 (U-box domain-containing protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1226.5 bits (3172), Expect = 0.0e+00
Identity = 630/735 (85.71%), Postives = 672/735 (91.43%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTD T+NVEAVSN+YSFKVHCRMCTELMKLVDRV EILPEIEAARPGSPEGREALCNLN
Sbjct: 1   MGTDSTKNVEAVSNIYSFKVHCRMCTELMKLVDRVLEILPEIEAARPGSPEGREALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
           IG  KAEL+LQYCRDSSKLYL LTGDRIISRCHRV++LLEHNLRKIKYMVPVALARKISQ
Sbjct: 61  IGMKKAELVLQYCRDSSKLYLALTGDRIISRCHRVKSLLEHNLRKIKYMVPVALARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           IADDL+VAKFILDSSEEEVWKAM+QLLKLG S PDA E SEIKALKIAALRLNI SYKE+
Sbjct: 121 IADDLKVAKFILDSSEEEVWKAMQQLLKLGASSPDAPEISEIKALKIAALRLNILSYKEM 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           LFE+RS+RKLLDDVGHG+P K+KILTYL Y LKK+G+LI+QEI+EARADISSSNGYGE+E
Sbjct: 181 LFERRSIRKLLDDVGHGNPRKRKILTYLFYRLKKYGKLILQEISEARADISSSNGYGEME 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
            NVRQ N+  QAD+ILNRAIPPEEFKCPIS+RLMYDPVVI SKVTYEKVWIEKWF+EGHD
Sbjct: 241 GNVRQGNDTPQADMILNRAIPPEEFKCPISLRLMYDPVVIESKVTYEKVWIEKWFKEGHD 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCP TKMKLT FSMTPNVDMK LI KWC+KF VTIPDPS+EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPLTKMKLTSFSMTPNVDMKKLIHKWCIKFRVTIPDPSVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           DIRLPIDFS+ISFGGL NSYYPDSLRL   N L+IK  QSK DDL R+QSDSNAEETNLE
Sbjct: 361 DIRLPIDFSDISFGGLHNSYYPDSLRL---NELSIKSRQSKGDDLGRFQSDSNAEETNLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPST+SELSWE K KVIKDM N +NKN  GPILSETVMDQLALF+KDA D QDSEAQKNG
Sbjct: 421 FPSTMSELSWESKSKVIKDMNNIINKNRFGPILSETVMDQLALFIKDAFDLQDSEAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
           SELFLSLVRK+RSNSL+VPEKILTTLASLLNSEVT EVLAILEA SGH KC S FVTSGV
Sbjct: 481 SELFLSLVRKSRSNSLSVPEKILTTLASLLNSEVTDEVLAILEALSGHHKCGSKFVTSGV 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDE-ALWGKCI 600
           LASMVEYLDSE+K LQEFA+KTF+NLSSNSHICSDIVSLGCIPKLV LLNDE  L GKC+
Sbjct: 541 LASMVEYLDSEIKALQEFAVKTFANLSSNSHICSDIVSLGCIPKLVSLLNDEDDLSGKCM 600

Query: 601 FILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVM 660
           FIL+NLCHTE+ +IYIVETNGCIASIV+ LEMG LE+QEHA+TILLSLCSQRVEYCELVM
Sbjct: 601 FILQNLCHTEDGKIYIVETNGCIASIVRCLEMGKLENQEHAITILLSLCSQRVEYCELVM 660

Query: 661 AEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRK 720
            EGVFPLLW ISNNGSEKGKAGAFEL RLL+DVQDNEQQESYVSD SSSNK A NS QRK
Sbjct: 661 EEGVFPLLWKISNNGSEKGKAGAFELHRLLKDVQDNEQQESYVSDTSSSNKPACNSKQRK 720

Query: 721 PSKKSGFLGRIFTKH 735
            SKK GFLG IFTKH
Sbjct: 721 VSKKPGFLGMIFTKH 732

BLAST of Tan0008725 vs. NCBI nr
Match: XP_023533238.1 (U-box domain-containing protein 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 626/735 (85.17%), Postives = 669/735 (91.02%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTD T+NVEAVSN+YSFKVHCRMCTELMKLVDRV E+LPEIEAARPGSPEGREALCNLN
Sbjct: 1   MGTDSTKNVEAVSNIYSFKVHCRMCTELMKLVDRVLEVLPEIEAARPGSPEGREALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
           IG  KAEL+LQYCRDSSKLYL LTGDRIISRCHRV++LLEHNLRKIKYMVPVALARKISQ
Sbjct: 61  IGMKKAELVLQYCRDSSKLYLALTGDRIISRCHRVKSLLEHNLRKIKYMVPVALARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           IADDL+VAKFILDSSEEEVWKAM+QLLKLG S PDA E SEIKALKIAALRLNI SYKE+
Sbjct: 121 IADDLKVAKFILDSSEEEVWKAMQQLLKLGASSPDAPEISEIKALKIAALRLNILSYKEM 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           LFE+RS+RKLLDDVGHG+P K+KILTYL Y LKK+G+LI+QEI+EARADISSSN YGE+E
Sbjct: 181 LFERRSIRKLLDDVGHGNPRKRKILTYLFYRLKKYGKLILQEISEARADISSSNQYGEME 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
            NVRQ N+  QAD+ILNRAIPPEEFKCPIS+RLMYDPVVI SKVTYEKVWIEKWF+EGHD
Sbjct: 241 GNVRQGNDTPQADMILNRAIPPEEFKCPISLRLMYDPVVIESKVTYEKVWIEKWFKEGHD 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCP TKMKLT FSMTPNVDMK LI KWC+KF VTIPDPS+EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPLTKMKLTSFSMTPNVDMKKLIHKWCIKFRVTIPDPSVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           DI LPIDFS+ISFGGL NSYYPDSLRL   N L IK  QSK DDL R+QSDSNAEET+LE
Sbjct: 361 DICLPIDFSDISFGGLHNSYYPDSLRL---NELGIKSRQSKGDDLGRFQSDSNAEETDLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPST+SELSWE K KVIKDM N +NKN  GPILSETVMDQLALF+KDA D QDSEAQKNG
Sbjct: 421 FPSTMSELSWESKSKVIKDMNNVINKNRFGPILSETVMDQLALFMKDAFDLQDSEAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
           SELFLSLVRK+RSNSL+VPEKILTTLASLLNSEVT EVLAILEA SGH KC S F TSGV
Sbjct: 481 SELFLSLVRKSRSNSLSVPEKILTTLASLLNSEVTDEVLAILEALSGHYKCGSKFATSGV 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDE-ALWGKCI 600
           LASMVEYLDSE+K LQEFA+KTF+NLSSNSHICSDIVSLGCIPKLV LLNDE  L GKC+
Sbjct: 541 LASMVEYLDSEIKALQEFAVKTFANLSSNSHICSDIVSLGCIPKLVSLLNDEDDLSGKCM 600

Query: 601 FILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVM 660
           FIL+NLCHTEE +IYIVETNGCIASIV+ LEMG LE+QEHA+TILLSLCSQRVEYCELVM
Sbjct: 601 FILQNLCHTEEGKIYIVETNGCIASIVRRLEMGKLENQEHAITILLSLCSQRVEYCELVM 660

Query: 661 AEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRK 720
           AEGVFPLLW ISNNGSEKGKAGAFEL RLL+DVQDNEQQESYVSD SSSNK A +S QRK
Sbjct: 661 AEGVFPLLWKISNNGSEKGKAGAFELHRLLKDVQDNEQQESYVSDTSSSNKPACHSKQRK 720

Query: 721 PSKKSGFLGRIFTKH 735
            SKK GFLG IFTKH
Sbjct: 721 VSKKPGFLGMIFTKH 732

BLAST of Tan0008725 vs. NCBI nr
Match: KAG7015308.1 (U-box domain-containing protein 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1212.6 bits (3136), Expect = 0.0e+00
Identity = 622/742 (83.83%), Postives = 666/742 (89.76%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTDGTENVEA SNV+SFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN
Sbjct: 1   MGTDGTENVEAASNVHSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
            GK KAELLLQYCR+SSKLYL LTGDRIISRCHRVRTLLEH+LRKIKYMVPV LARKISQ
Sbjct: 61  NGKMKAELLLQYCRESSKLYLALTGDRIISRCHRVRTLLEHSLRKIKYMVPVTLARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           +ADDLRVAKFILDSS+EE WKA+RQLLKLG SPPD LE SEI  LKIAALRLNISS KE+
Sbjct: 121 MADDLRVAKFILDSSDEEAWKALRQLLKLGASPPDVLEKSEIATLKIAALRLNISSPKEM 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           L+EKRS+RKLLDDVGHGDPPKKKILTYLLY+LKKHGELIVQEI E +AD S+SNG+GEIE
Sbjct: 181 LYEKRSIRKLLDDVGHGDPPKKKILTYLLYILKKHGELIVQEIRENQADSSNSNGHGEIE 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
           VNV+  NNAS AD+ILNRAIPPEEFKCPISMRLMYDPVVIAS VTYEKVWIEKWFEEGH+
Sbjct: 241 VNVKHGNNASHADMILNRAIPPEEFKCPISMRLMYDPVVIASGVTYEKVWIEKWFEEGHN 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCP TKMKLTDFSM PNVD+KN+IDKWC+KFGVTIPDP++EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPHTKMKLTDFSMIPNVDLKNVIDKWCIKFGVTIPDPTVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           DIRLPIDFSNIS GGLDNSYYPDSLRLNGGN LAIK GQSKD DL R+QSDS AEE +LE
Sbjct: 361 DIRLPIDFSNISLGGLDNSYYPDSLRLNGGNELAIKSGQSKDHDLQRFQSDSYAEEADLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPST++ELSWE KCK+IKDMK  +NK GVGP LSETVMD LALFLKDACDQQDSEAQKNG
Sbjct: 421 FPSTMNELSWESKCKIIKDMKVVINKKGVGPALSETVMDHLALFLKDACDQQDSEAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
           +ELFLSLVR++RSN L VPEK+LTTLASLLNS VTYEVLAILEA SGHRKC SNFVTSGV
Sbjct: 481 AELFLSLVRRSRSNRLIVPEKVLTTLASLLNSVVTYEVLAILEALSGHRKCGSNFVTSGV 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDEALWGKCIF 600
           LAS+ +YLDSE+ DLQEFAIKTF NLSSNS ICS+IVSLGCIPKLVPLLN E L GKCIF
Sbjct: 541 LASIAKYLDSEVGDLQEFAIKTFYNLSSNSDICSNIVSLGCIPKLVPLLNYENLSGKCIF 600

Query: 601 ILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVMA 660
           ILKNLCHTE+ARI IVETNGCI+SI Q L +GSLEDQEHAVTILLSLCSQR EYCELVM 
Sbjct: 601 ILKNLCHTEDARISIVETNGCISSIAQCLWLGSLEDQEHAVTILLSLCSQRFEYCELVME 660

Query: 661 EGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRKP 720
           EGV P L +I+  GSEKGKAGA EL RLLRD QDNEQQE  VS+P  S +   NS+Q KP
Sbjct: 661 EGVIPPLSSITMKGSEKGKAGATELLRLLRDFQDNEQQEPCVSEPPYSYEPPCNSEQEKP 720

Query: 721 SKKSGFLG---RIFTKHSPLKK 740
           SKKSGFLG    IF+K   LKK
Sbjct: 721 SKKSGFLGMNISIFSKRGLLKK 742

BLAST of Tan0008725 vs. ExPASy TrEMBL
Match: A0A6J1FSH4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447749 PE=4 SV=1)

HSP 1 Score: 1228.0 bits (3176), Expect = 0.0e+00
Identity = 631/735 (85.85%), Postives = 671/735 (91.29%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTD T+NVEAVSN+YSFKVHCRMCTELMKLVDRV EILPEIEAARPGSPEGREALCNLN
Sbjct: 1   MGTDSTKNVEAVSNIYSFKVHCRMCTELMKLVDRVLEILPEIEAARPGSPEGREALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
           IG  KAEL+LQYCRDSSKLYL LTGDRIISRCHRV++LLEHNLRKIKYMVPVALARKISQ
Sbjct: 61  IGMKKAELVLQYCRDSSKLYLALTGDRIISRCHRVKSLLEHNLRKIKYMVPVALARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           IADDL+VAKFILDSSEEEVWKAM+QLLKLG S PDA E SEIKALKIAALRLNI SYKE+
Sbjct: 121 IADDLKVAKFILDSSEEEVWKAMQQLLKLGPSSPDAPEISEIKALKIAALRLNILSYKEM 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           LFE+RS+RKLLDDVGHG+P K+KILTYL Y LKK+G+LI+QEI+EARADISSSNGYGE+E
Sbjct: 181 LFERRSIRKLLDDVGHGNPRKRKILTYLFYRLKKYGKLILQEISEARADISSSNGYGEME 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
            NVRQ N+  QAD+ILNRAIPPEEFKCPIS+RLMYDPVVI SKVTYEKVWIEKWF EGHD
Sbjct: 241 GNVRQGNDTPQADMILNRAIPPEEFKCPISLRLMYDPVVIESKVTYEKVWIEKWFNEGHD 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCP TKMKLT FSMTPNVDMK LI KWC+KF VTIPDPS+EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPLTKMKLTSFSMTPNVDMKKLIHKWCIKFRVTIPDPSVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           DIRLPIDFS+ISFGGL NSYYPDSLRL   N L IK  QSKDDDL R+QSDSNAEETNLE
Sbjct: 361 DIRLPIDFSDISFGGLHNSYYPDSLRL---NELGIKSRQSKDDDLGRFQSDSNAEETNLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPST+SELSWE K KVIKDM + +NKN  GPILSETVMDQLALF+KDA D QDSEAQKNG
Sbjct: 421 FPSTMSELSWESKSKVIKDMNDIINKNRFGPILSETVMDQLALFIKDAFDLQDSEAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
           SELFLSLVRK+RSNSL+VPEKILTTLASLLNSEVT EVLAILEA SGH KC S FVTSGV
Sbjct: 481 SELFLSLVRKSRSNSLSVPEKILTTLASLLNSEVTDEVLAILEALSGHHKCGSKFVTSGV 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDE-ALWGKCI 600
           LASMVEYLDSE+K LQEFA+KTF+NLSSNSHICSDIVSLGCIPKLV LLNDE  L GKC+
Sbjct: 541 LASMVEYLDSEIKALQEFAVKTFANLSSNSHICSDIVSLGCIPKLVSLLNDEDDLSGKCM 600

Query: 601 FILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVM 660
           FIL+NLCHTEE +IYIVETNGCIASIV+ LEMG LE+QEHA+TILLSLCSQRVEYCELVM
Sbjct: 601 FILQNLCHTEEGKIYIVETNGCIASIVRCLEMGKLENQEHAITILLSLCSQRVEYCELVM 660

Query: 661 AEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRK 720
            EGVFPLLW ISNNGSEKGKAGAFEL RLL+DVQDNEQQESYVSD SSSNK A NS QRK
Sbjct: 661 EEGVFPLLWKISNNGSEKGKAGAFELHRLLKDVQDNEQQESYVSDTSSSNKPACNSKQRK 720

Query: 721 PSKKSGFLGRIFTKH 735
            SKK GFLG IFTKH
Sbjct: 721 VSKKPGFLGMIFTKH 732

BLAST of Tan0008725 vs. ExPASy TrEMBL
Match: A0A6J1EMP9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111435989 PE=4 SV=1)

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 621/743 (83.58%), Postives = 667/743 (89.77%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTDGTENVEA SNV+SFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN
Sbjct: 1   MGTDGTENVEAASNVHSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
            GK KAELLLQYCR+SSKLYL LTGDRIISRCHRVRTLLEH+LRKIKYMVPV LARKISQ
Sbjct: 61  NGKMKAELLLQYCRESSKLYLALTGDRIISRCHRVRTLLEHSLRKIKYMVPVTLARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           +ADDLRVAKFILDSS+EE WKA+RQLLKLG SPPD LE SEI  LKIAALRLNISS KE+
Sbjct: 121 MADDLRVAKFILDSSDEEAWKALRQLLKLGASPPDVLEKSEIATLKIAALRLNISSPKEM 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           L+EKRS+RKLLDDVGHGDPPKKKILTYLLY+LKKHGELI+QEI E +AD S+SNG+GEIE
Sbjct: 181 LYEKRSIRKLLDDVGHGDPPKKKILTYLLYILKKHGELILQEIRENQADSSNSNGHGEIE 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
           VNV+  NNAS AD+ILNRAIPPEEFKCPISMRLMYDPVVIAS VTYEKVWIEKWFEEGH+
Sbjct: 241 VNVKHGNNASHADMILNRAIPPEEFKCPISMRLMYDPVVIASGVTYEKVWIEKWFEEGHN 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCP TKMKLTDFSM PNVD+KN+IDKWC+KFGVTIPDP++EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPHTKMKLTDFSMIPNVDLKNVIDKWCIKFGVTIPDPTVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           DIRLPIDFSN+S GGLDNSYYPDSLRLNGGN LAIK GQSKD DL R+QSDS AEE +LE
Sbjct: 361 DIRLPIDFSNMSLGGLDNSYYPDSLRLNGGNELAIKAGQSKDHDLQRFQSDSYAEEADLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPSTI+ELSWE KCK+IKDMK  +NK GVGP LSETV+D LALFLKDACDQQDS+AQKNG
Sbjct: 421 FPSTINELSWESKCKIIKDMKVVINKKGVGPALSETVVDHLALFLKDACDQQDSKAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
           +ELFLSLVR++RSN L VPEK+LTTLASLLNS VTYEVLAILEA SGHRKC SNFVTSGV
Sbjct: 481 AELFLSLVRRSRSNRLIVPEKVLTTLASLLNSVVTYEVLAILEALSGHRKCGSNFVTSGV 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDEALWGKCIF 600
           LAS+ +YLDSE+ DLQEFAIKTF NLSSNS ICSDIVSLGCIPKLVPLLN E L GKCIF
Sbjct: 541 LASIAKYLDSEVGDLQEFAIKTFYNLSSNSDICSDIVSLGCIPKLVPLLNYENLSGKCIF 600

Query: 601 ILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVMA 660
           ILKNLCHTE+ARI IVETNGCI+SI Q L +GSLEDQEHAVTILLSLCSQR EYCELVM 
Sbjct: 601 ILKNLCHTEDARISIVETNGCISSIAQCLWLGSLEDQEHAVTILLSLCSQRFEYCELVME 660

Query: 661 EGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRKP 720
           EGV P L +I+  GSEKGKAGA EL RLLRD QDNEQQE  VS+P  S +   NS+Q KP
Sbjct: 661 EGVIPPLSSITMKGSEKGKAGATELLRLLRDFQDNEQQEPCVSEPPYSYEPPCNSEQGKP 720

Query: 721 SKKSGFLG---RIFTKHSPLKKK 741
           SKKSGFLG    IF+K   LKKK
Sbjct: 721 SKKSGFLGMNISIFSKRGLLKKK 743

BLAST of Tan0008725 vs. ExPASy TrEMBL
Match: A0A6J1IRZ1 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478780 PE=4 SV=1)

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 623/739 (84.30%), Postives = 669/739 (90.53%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTD T+ VEAVSN+YSFKVHCRMCTELMKLVDRV EILPEIEAARPGSPEGREALCNLN
Sbjct: 1   MGTDSTKKVEAVSNIYSFKVHCRMCTELMKLVDRVLEILPEIEAARPGSPEGREALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
           IG  KAEL+LQYCRDSSKLYL LTGDRIISRCHRV++LLEHNLRKIKYMVPVALARKISQ
Sbjct: 61  IGMKKAELVLQYCRDSSKLYLALTGDRIISRCHRVKSLLEHNLRKIKYMVPVALARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           IADDL++AKFILDSSEEEVWKAM+QLLKLG S PDA E SEIKALKIAALRLNI SYKE+
Sbjct: 121 IADDLKIAKFILDSSEEEVWKAMQQLLKLGPSSPDAPEISEIKALKIAALRLNILSYKEM 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           LFE+RS+RKLLDDVGHG+P K+KILTYL Y LKK+G+LI+QEI+EARADISSSNGYGE+E
Sbjct: 181 LFERRSIRKLLDDVGHGNPRKRKILTYLFYRLKKYGKLILQEISEARADISSSNGYGEME 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
            NVRQ N+  QAD+ILNRAIPPEEFKCPIS+RLMYDPVV+ SKVTYEKV IEKWF+EGHD
Sbjct: 241 GNVRQGNDTPQADMILNRAIPPEEFKCPISLRLMYDPVVVESKVTYEKVRIEKWFKEGHD 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCP TKMKLT+FSMTPNVDMK LI KWC+KF VTIPDPS+EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPLTKMKLTNFSMTPNVDMKKLIHKWCIKFRVTIPDPSVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           +IRLPIDFS+ISFGGL NSYYPDSLRL   N L IK  QSKDDDL R+QSDSNAEETNLE
Sbjct: 361 EIRLPIDFSDISFGGLHNSYYPDSLRL---NELGIKSRQSKDDDLGRFQSDSNAEETNLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPST+SELSWE K KVIKDM + +NKN  GPILSETVMDQLALF+KDA D QDSEAQKNG
Sbjct: 421 FPSTMSELSWESKSKVIKDMNDVINKNRFGPILSETVMDQLALFIKDAFDLQDSEAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
           SELFLSLVRK+RSNSL+VPEKILTTLASLLNSEVT EVLAILEA SGH KC S F+TSGV
Sbjct: 481 SELFLSLVRKSRSNSLSVPEKILTTLASLLNSEVTDEVLAILEALSGHHKCGSKFITSGV 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDE-ALWGKCI 600
           LASMVEYLD E+K LQEFA+KTF+NLSSNSHI SDIVSLGCIPKLV LLNDE  L  KC+
Sbjct: 541 LASMVEYLDLEIKALQEFAVKTFANLSSNSHIRSDIVSLGCIPKLVSLLNDEDDLSEKCM 600

Query: 601 FILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVM 660
           FIL+NLC TEE +IYIVETNGCIASIV+ LEMG LE+QEHA+TILLSLCSQRVEYCELVM
Sbjct: 601 FILQNLCQTEEGKIYIVETNGCIASIVRRLEMGKLENQEHAITILLSLCSQRVEYCELVM 660

Query: 661 AEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRK 720
           AEGVFPLLW ISNNGSEKGKAGAFEL RLL+DVQD EQQESYVSD SSSNK A NS QRK
Sbjct: 661 AEGVFPLLWKISNNGSEKGKAGAFELHRLLKDVQDKEQQESYVSDTSSSNKPACNSKQRK 720

Query: 721 PSKKSGFLGRIFTKHSPLK 739
            SKK GFLG IFTKH P K
Sbjct: 721 VSKKPGFLGMIFTKHRPPK 736

BLAST of Tan0008725 vs. ExPASy TrEMBL
Match: A0A6J1J967 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111482874 PE=4 SV=1)

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 616/743 (82.91%), Postives = 661/743 (88.96%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTD TENVEA SNV+SFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN
Sbjct: 1   MGTDSTENVEAASNVHSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
            GK KAELLLQYCR+SSK+YL LTGDRIISRCHRVRTLLEH+LRKIKYMVPV LARKISQ
Sbjct: 61  NGKMKAELLLQYCRESSKVYLALTGDRIISRCHRVRTLLEHSLRKIKYMVPVTLARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           +ADDLRVAKFILDSS+EE WKA+RQLLKLG SPPD LE SEI  LKIAALRLNISS KE+
Sbjct: 121 MADDLRVAKFILDSSDEEAWKALRQLLKLGASPPDVLEKSEIATLKIAALRLNISSPKEM 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           L+EKRS+RKLLDDVGHGDPPKKKILTYLLY+LKKHGELI+QEI E +AD S+SNG+ EIE
Sbjct: 181 LYEKRSIRKLLDDVGHGDPPKKKILTYLLYILKKHGELILQEIRENQADSSNSNGHREIE 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
           VNV+  NNAS AD+ILNRA PPEEFKCPISMRLMYDPVVIAS VTYEK WIEKWFEEGH+
Sbjct: 241 VNVKHGNNASHADMILNRATPPEEFKCPISMRLMYDPVVIASGVTYEKAWIEKWFEEGHN 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCP TKMKLTDFSM PNVD+KN+IDKWC+KFGVTIPDP++EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPHTKMKLTDFSMIPNVDLKNVIDKWCIKFGVTIPDPTVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           DIRLPIDFSNIS GGLDNSYYPDSLRLNGGN LAIK GQSKD DL R+QSDS AEE +LE
Sbjct: 361 DIRLPIDFSNISLGGLDNSYYPDSLRLNGGNELAIKSGQSKDHDLQRFQSDSYAEEADLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPSTI+ELSWE KCK+IKDMK  +NK GVGP LSETV+D LALFLKDACDQQDSEAQKNG
Sbjct: 421 FPSTINELSWESKCKIIKDMKVVINKKGVGPALSETVVDHLALFLKDACDQQDSEAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
           +ELFLSLVR++RSN L VPEK+LTTLASLLNS VTYEVLAILEA SGHRKC SNFVTSGV
Sbjct: 481 AELFLSLVRRSRSNRLIVPEKVLTTLASLLNSVVTYEVLAILEALSGHRKCGSNFVTSGV 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDEALWGKCIF 600
           LAS+ +YLDSE+ DLQEFAIKTF NLSSN  ICSDIVSLGCIPKLVPLLN E L GKCIF
Sbjct: 541 LASIAKYLDSEVGDLQEFAIKTFYNLSSNGDICSDIVSLGCIPKLVPLLNYENLSGKCIF 600

Query: 601 ILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVMA 660
           ILKNLCHTE+ARI IVETNGCI+SI Q L +GSLEDQEHAVTILLSLCSQR EYCELVM 
Sbjct: 601 ILKNLCHTEDARISIVETNGCISSIAQCLWLGSLEDQEHAVTILLSLCSQRFEYCELVME 660

Query: 661 EGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRKP 720
           EGV P L +I+  GSEKGKAGA EL RLLRD QDNEQQE  VS+P  S +   NS+Q KP
Sbjct: 661 EGVIPPLSSITMKGSEKGKAGATELLRLLRDFQDNEQQEPCVSEPPYSYEPPCNSEQGKP 720

Query: 721 SKKSGFLG---RIFTKHSPLKKK 741
           SKKSGFLG    IF+K   L KK
Sbjct: 721 SKKSGFLGMNISIFSKRGLLTKK 743

BLAST of Tan0008725 vs. ExPASy TrEMBL
Match: A0A0A0KWP7 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G046720 PE=4 SV=1)

HSP 1 Score: 1190.3 bits (3078), Expect = 0.0e+00
Identity = 615/739 (83.22%), Postives = 657/739 (88.90%), Query Frame = 0

Query: 1   MGTDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLN 60
           MGTD TENVEAVSN +SFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGR+ALCNLN
Sbjct: 1   MGTDTTENVEAVSNAHSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGRQALCNLN 60

Query: 61  IGKNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQ 120
            GK KAELLLQYCRDSSKLYL LTGDRI+SRCHRVRTLLEHNLRKIKYMV VALARKISQ
Sbjct: 61  EGKMKAELLLQYCRDSSKLYLALTGDRIVSRCHRVRTLLEHNLRKIKYMVSVALARKISQ 120

Query: 121 IADDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEV 180
           IADDLRVAKFILDSSEEE WK MRQLLKLG  PPD LE SEIKALK AALRLNI S KE+
Sbjct: 121 IADDLRVAKFILDSSEEEAWKDMRQLLKLGALPPDELEKSEIKALKTAALRLNILSSKEL 180

Query: 181 LFEKRSLRKLLDDVGHGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIE 240
           L+E+RS+RKLLDDVG  DPPKKKILTYLLYLLKKHGELI+QEI E +AD SSSN YGEI+
Sbjct: 181 LYERRSIRKLLDDVGRDDPPKKKILTYLLYLLKKHGELILQEIRETQADRSSSNEYGEIK 240

Query: 241 VNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHD 300
            N R RN ASQADIILNRAIPPEEFKCPISMRLMYDPVVIAS VTYEKVWIEKWF EGHD
Sbjct: 241 ANARHRNYASQADIILNRAIPPEEFKCPISMRLMYDPVVIASGVTYEKVWIEKWFLEGHD 300

Query: 301 TCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASFGSSMN 360
           TCPQTKMKL D S+TPNVD+KNLI+KWC+KFGVTIPDPS+EPECPEVWENSIASFGSSMN
Sbjct: 301 TCPQTKMKLADCSVTPNVDLKNLINKWCIKFGVTIPDPSVEPECPEVWENSIASFGSSMN 360

Query: 361 DIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAIKYGQSKDDDLHRYQSDSNAEETNLE 420
           DI LPIDFSNIS GGLDNSYYPD LRLNGGN LAIK GQSK+DD  R+QS SNAEE +LE
Sbjct: 361 DIHLPIDFSNISLGGLDNSYYPDLLRLNGGNELAIKSGQSKEDDQQRFQSGSNAEEIDLE 420

Query: 421 FPSTISELSWELKCKVIKDMKNALNKNGVGPILSETVMDQLALFLKDACDQQDSEAQKNG 480
           FPST+SELSWE KCKV+KDMK ++NKNGVG  LSETV+DQLALFLKDAC+Q+DSEAQKNG
Sbjct: 421 FPSTMSELSWESKCKVMKDMKVSMNKNGVGLTLSETVLDQLALFLKDACNQRDSEAQKNG 480

Query: 481 SELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTYEVLAILEAFSGHRKCSSNFVTSGV 540
            ELFLSLVR++RSN L+VPEK+L +LASLLNSEVTYEVLAILEA SGHRKCSS+FVTSG 
Sbjct: 481 CELFLSLVRRSRSNRLSVPEKVLKSLASLLNSEVTYEVLAILEAISGHRKCSSSFVTSGA 540

Query: 541 LASMVEYLDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDEALWGKCIF 600
           L S+ +YLDSE++DLQEFAIKT  NLS NS ICSDIVSLGCIPKLVPLLN     GKCIF
Sbjct: 541 LTSLAKYLDSEIEDLQEFAIKTLYNLSMNSDICSDIVSLGCIPKLVPLLNYGNFSGKCIF 600

Query: 601 ILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVMA 660
           ILKNLCHTEEARI IV TNGCI+SI Q L MGSLEDQEHAVTILLSLCSQRVEYCELVM 
Sbjct: 601 ILKNLCHTEEARISIVGTNGCISSIAQRLGMGSLEDQEHAVTILLSLCSQRVEYCELVME 660

Query: 661 EGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQRKP 720
           EGV P L  IS  GSEKGKAGA EL RLLRDVQDNE Q+S VS+PSSS +   NS+QR P
Sbjct: 661 EGVIPPLCTISMKGSEKGKAGATELLRLLRDVQDNEPQDSCVSEPSSSYEPPCNSEQRTP 720

Query: 721 SKKSGFLGRIFTKHSPLKK 740
           SKKSGFLG IF+K +P KK
Sbjct: 721 SKKSGFLG-IFSKRTPRKK 738

BLAST of Tan0008725 vs. TAIR 10
Match: AT4G36550.1 (ARM repeat superfamily protein )

HSP 1 Score: 382.9 bits (982), Expect = 5.9e-106
Identity = 266/748 (35.56%), Postives = 399/748 (53.34%), Query Frame = 0

Query: 17  SFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLNIGKNKAELLLQYCRDS 76
           S+K+H  MC EL +LVDR+  I P+IE ARPG   G + LC L+   +K + LLQYC +S
Sbjct: 12  SYKMHSSMCLELKRLVDRIMRIFPDIEDARPGCSSGIQTLCLLHNALDKTKQLLQYCSES 71

Query: 77  SKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQIADDLRVAKFILDSSE 136
           SKLY+ +TGD I++R  R +  LE  L  I+ +VP  L  KISQI  DLR  +  L+ SE
Sbjct: 72  SKLYMAVTGDAILARGSRAKKSLEQCLNDIRSIVPTILEIKISQIVQDLRSTQLTLEFSE 131

Query: 137 EEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEVLFEKRSLRKLLDDVGH 196
           EE  KA+R+L++   S   +    EIK    AAL+L +S+ + ++ E+RSL+ + +D   
Sbjct: 132 EEAGKAIRELMQ--KSTSSSASPDEIKDFHYAALKLQLSTPEAIVTERRSLKIICED--- 191

Query: 197 GDPPKKKILTYLLYLLKKHGELIVQEITEARADISSSNGYGEIEVNVRQRNNASQADIIL 256
               K+   T+                      I  S      E    + +N +      
Sbjct: 192 ---HKQNSFTH-------------------HQSIDDSLHANAAEAEASEEHNGTL----- 251

Query: 257 NRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWFEEGHDTCPQTKMKLTDFSMTP 316
                PE+FKC +S  +MYDPV+I+S  T+E++ I+KWF+EG+D+CP +K KL DF++ P
Sbjct: 252 -----PEKFKCTLSRTVMYDPVIISSGNTFERMQIQKWFDEGNDSCPISKRKLDDFTLKP 311

Query: 317 NVDMKNLIDKWCVKFGVTIPDPS---LEPECPEVWENSIASFGSSMNDIRLPIDFSNISF 376
           NV++K+ I +WC K G+ + DP+   ++      +  SIASFGSS+ +I    D S IS 
Sbjct: 312 NVELKSQISEWCAKNGLDVQDPARKHVKASNSIDFSVSIASFGSSLYNIP---DHSGISI 371

Query: 377 GGLDNSYYPDSLRLNG-GNGLAIKYGQSKDDDLHRYQSDSNAEETNLEFPSTISELSWEL 436
              ++SY  DS   +    G      Q  D       +DS+  E  ++    ++ L W+ 
Sbjct: 372 TDFNSSYSIDSSSYSKMSKGGYFTPMQRIDSASGAGDTDSSHSEIEIDPLCGLTNLPWDA 431

Query: 437 KCKVIKDMKNAL--NKNGVGPILSETVMDQLALFLKDACDQQDS--EAQKNGSELFLSLV 496
           + KV++D+++    +      +     ++ L  +LK+A ++  +  E  K G +L L+ +
Sbjct: 432 QIKVVEDVRSRFEHSTRAFRSMSPSKFLEPLITYLKNALERNGTAGEIIKGGLDLLLAFL 491

Query: 497 RKNRSNSLNVPEKILTTLASLLNSE-VTYEVLAILEAFSGHRKCSSNFVTSGVLASMVEY 556
             NR    ++ E++    +  L SE V  E L ILE  S H    S   +SG L+S+++ 
Sbjct: 492 SGNRRAIESLEEEVFKMFSVFLESEVVAEEALNILEVLSNHPHGPSKITSSGSLSSLLKI 551

Query: 557 LDSEMKDLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLVPLLNDEALWGKCIFILKNLCH 616
           ++S+ + LQE A+ T  NLSS+  IC ++VSL  I KL   L  +      I ILKNLC 
Sbjct: 552 VESQAEHLQEQAMITLKNLSSSMEICLEMVSLDFIQKLTSFLQQKVFCKHSIIILKNLCS 611

Query: 617 TEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTILLSLCSQRVEYCELVMAEG--VFP 676
           TE+ R  I ET  C+ASI +LLE    E+QE+A++ILL LC Q++EYC LV+ E   ++ 
Sbjct: 612 TEKGRGCITETPDCLASIAELLESNVPEEQENAISILLQLCVQKIEYCCLVVREATDIYS 671

Query: 677 LLWNISNNGSEKGKAGAFELRRLLRDVQDNEQQESYVSDPSSSNKAASNSDQ-------- 736
            L  ISNNG+E+ K  A EL R L +V  ++++E  VS        AS + Q        
Sbjct: 672 SLILISNNGTEEVKVSASELLRALVEVDSDKEEEEEVSSRPEGRTTASPTSQVVTPVTHP 716

Query: 737 -----RKPSKKSGFLGRIFTKHSPLKKK 741
                    KKSG  G  F   S LKKK
Sbjct: 732 EPVKITPSPKKSGLFGFNF---SSLKKK 716

BLAST of Tan0008725 vs. TAIR 10
Match: AT1G67530.1 (ARM repeat superfamily protein )

HSP 1 Score: 307.0 bits (785), Expect = 4.1e-83
Identity = 237/751 (31.56%), Postives = 391/751 (52.06%), Query Frame = 0

Query: 3   TDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLNIG 62
           T+  EN+ A S+    K+H  MC EL  ++ +V  I P +E ARP S  G +ALC+L+I 
Sbjct: 4   TELEENLFAASDA---KLHGDMCKELSGVLCKVLSIFPSLEGARPRSKSGIQALCSLHIA 63

Query: 63  KNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQIA 122
             KA+ +LQ+C + SKLYL +TGD ++ +  + +  L   L++++ +VP ++  +I +I 
Sbjct: 64  LEKAKNILQHCSECSKLYLAITGDAVLLKFEKAKIALIDGLKRVEDIVPSSIGSQILEIV 123

Query: 123 DDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEVLF 182
            +L   +F+LD SE+EV   +  LL+ G    +  +N+E++    AA RL+I+S +  L 
Sbjct: 124 GELENTRFMLDPSEKEVGDQIIALLQQGKKFDNCNDNTELEIFHRAATRLSITSSRVALA 183

Query: 183 EKRSLRKLLDDV-GHGDPPKKKILTYLLYLLKKHGELIVQEITE-----------ARADI 242
           E+R+L+KL+D      D  K+ I+ YLL+L++K  +L   EI +              D 
Sbjct: 184 ERRALKKLIDRARAEEDKRKESIVAYLLHLMRKCSKLFRSEILDENDSPGSYPCSPNEDH 243

Query: 243 SSSNGYGEIEVNVRQRNNASQADIILNR-AIPPEEFKCPISMRLMYDPVVIASKVTYEKV 302
            S +G+G         N+     I   +  +PPEE +CPIS++LM DPV+IAS  TYE+V
Sbjct: 244 GSVHGFGRQLSRFGSLNDKPMNSINSGQMPVPPEELRCPISLQLMCDPVIIASGQTYERV 303

Query: 303 WIEKWFEEGHDTCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWE 362
            IEKWF +GH+TCP+T+ +L   S+TPN  +K LI  WC + G  IP    E +  + W 
Sbjct: 304 CIEKWFSDGHNTCPKTQQQLPHISLTPNNCVKGLIASWCEQNGTQIPSGPPESQDLDYWR 363

Query: 363 NSIASFGSSMNDIRLPIDFSNISFGGLDNSYYPDSLRL--NGGNGLAIKYGQSKD----- 422
            +++   S+ +        S  S G    SY    +++     NG  +   Q+ +     
Sbjct: 364 LALSDSESTKSQ-------SVNSIG----SYKLKGVKIVPLEENGTTVVERQNTEESFVS 423

Query: 423 DDLHRYQSDSNAEETNLEFPSTIS-ELSWELKCKVIKDMKNALNKNGVGPIL--SETVMD 482
           DD     SD N  E   +  + ++ E   E KCKV++ ++  L  +    I   +   ++
Sbjct: 424 DDDDEEDSDINVLERYQDLLAVLNEEEGLEKKCKVVEKIRLLLKDDEEARIFMGANGFVE 483

Query: 483 QLALFLKDACDQQDSEAQKNGS-ELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTY-E 542
            L  FL  A D  ++ AQ +G+  LF   V  NR+  L +   ++  L  +++S  ++  
Sbjct: 484 ALLRFLGSAVDDNNAAAQDSGAMALFNLAVNNNRNKELMLTSGVIRLLEKMISSAESHGS 543

Query: 543 VLAILEAFSGHRKCSSNFVTSGVLASMVEYLDSEMKDLQEF-AIKTFSNLSSNSHICSDI 602
             A+    S   +  S   +S  +  +V+ L  E++   +  A+    NLS+ S     +
Sbjct: 544 ATALYLNLSCLDEAKSVIGSSQAVPFLVQLLQKEIETQCKLDALHALYNLSTYSPNIPAL 603

Query: 603 VSLGCIPKLVPLL--NDEALW-GKCIFILKNLCHTEEARIYIVETNGCIASIVQLLEMGS 662
           +S   I  L  LL    E LW  K + +L NL  ++E +   V + G I+S+  +L+MG 
Sbjct: 604 LSSNIIKSLQGLLASTGENLWIEKSLAVLLNLASSQEGKDEAVSSQGMISSLATVLDMGD 663

Query: 663 LEDQEHAVTILLSLCSQRVEYCELVMAEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQ 722
             +QE AV+ LL LC+ R    ++V+ EGV P L +IS NG+ +G+  + +L  L R+  
Sbjct: 664 TTEQEQAVSCLLILCNGRESCIQMVLQEGVIPSLVSISVNGTPRGREKSQKLLMLFRE-- 723

Query: 723 DNEQQESYVSDPSSSNKAASNSDQRKPSKKS 725
             E+Q+    D  SSN+      Q++P++KS
Sbjct: 724 --ERQQ---RDQPSSNR--DEPPQKEPARKS 731

BLAST of Tan0008725 vs. TAIR 10
Match: AT1G67530.2 (ARM repeat superfamily protein )

HSP 1 Score: 307.0 bits (785), Expect = 4.1e-83
Identity = 237/751 (31.56%), Postives = 391/751 (52.06%), Query Frame = 0

Query: 3   TDGTENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLNIG 62
           T+  EN+ A S+    K+H  MC EL  ++ +V  I P +E ARP S  G +ALC+L+I 
Sbjct: 4   TELEENLFAASDA---KLHGDMCKELSGVLCKVLSIFPSLEGARPRSKSGIQALCSLHIA 63

Query: 63  KNKAELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQIA 122
             KA+ +LQ+C + SKLYL +TGD ++ +  + +  L   L++++ +VP ++  +I +I 
Sbjct: 64  LEKAKNILQHCSECSKLYLAITGDAVLLKFEKAKIALIDGLKRVEDIVPSSIGSQILEIV 123

Query: 123 DDLRVAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEVLF 182
            +L   +F+LD SE+EV   +  LL+ G    +  +N+E++    AA RL+I+S +  L 
Sbjct: 124 GELENTRFMLDPSEKEVGDQIIALLQQGKKFDNCNDNTELEIFHRAATRLSITSSRVALA 183

Query: 183 EKRSLRKLLDDV-GHGDPPKKKILTYLLYLLKKHGELIVQEITE-----------ARADI 242
           E+R+L+KL+D      D  K+ I+ YLL+L++K  +L   EI +              D 
Sbjct: 184 ERRALKKLIDRARAEEDKRKESIVAYLLHLMRKCSKLFRSEILDENDSPGSYPCSPNEDH 243

Query: 243 SSSNGYGEIEVNVRQRNNASQADIILNR-AIPPEEFKCPISMRLMYDPVVIASKVTYEKV 302
            S +G+G         N+     I   +  +PPEE +CPIS++LM DPV+IAS  TYE+V
Sbjct: 244 GSVHGFGRQLSRFGSLNDKPMNSINSGQMPVPPEELRCPISLQLMCDPVIIASGQTYERV 303

Query: 303 WIEKWFEEGHDTCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWE 362
            IEKWF +GH+TCP+T+ +L   S+TPN  +K LI  WC + G  IP    E +  + W 
Sbjct: 304 CIEKWFSDGHNTCPKTQQQLPHISLTPNNCVKGLIASWCEQNGTQIPSGPPESQDLDYWR 363

Query: 363 NSIASFGSSMNDIRLPIDFSNISFGGLDNSYYPDSLRL--NGGNGLAIKYGQSKD----- 422
            +++   S+ +        S  S G    SY    +++     NG  +   Q+ +     
Sbjct: 364 LALSDSESTKSQ-------SVNSIG----SYKLKGVKIVPLEENGTTVVERQNTEESFVS 423

Query: 423 DDLHRYQSDSNAEETNLEFPSTIS-ELSWELKCKVIKDMKNALNKNGVGPIL--SETVMD 482
           DD     SD N  E   +  + ++ E   E KCKV++ ++  L  +    I   +   ++
Sbjct: 424 DDDDEEDSDINVLERYQDLLAVLNEEEGLEKKCKVVEKIRLLLKDDEEARIFMGANGFVE 483

Query: 483 QLALFLKDACDQQDSEAQKNGS-ELFLSLVRKNRSNSLNVPEKILTTLASLLNSEVTY-E 542
            L  FL  A D  ++ AQ +G+  LF   V  NR+  L +   ++  L  +++S  ++  
Sbjct: 484 ALLRFLGSAVDDNNAAAQDSGAMALFNLAVNNNRNKELMLTSGVIRLLEKMISSAESHGS 543

Query: 543 VLAILEAFSGHRKCSSNFVTSGVLASMVEYLDSEMKDLQEF-AIKTFSNLSSNSHICSDI 602
             A+    S   +  S   +S  +  +V+ L  E++   +  A+    NLS+ S     +
Sbjct: 544 ATALYLNLSCLDEAKSVIGSSQAVPFLVQLLQKEIETQCKLDALHALYNLSTYSPNIPAL 603

Query: 603 VSLGCIPKLVPLL--NDEALW-GKCIFILKNLCHTEEARIYIVETNGCIASIVQLLEMGS 662
           +S   I  L  LL    E LW  K + +L NL  ++E +   V + G I+S+  +L+MG 
Sbjct: 604 LSSNIIKSLQGLLASTGENLWIEKSLAVLLNLASSQEGKDEAVSSQGMISSLATVLDMGD 663

Query: 663 LEDQEHAVTILLSLCSQRVEYCELVMAEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQ 722
             +QE AV+ LL LC+ R    ++V+ EGV P L +IS NG+ +G+  + +L  L R+  
Sbjct: 664 TTEQEQAVSCLLILCNGRESCIQMVLQEGVIPSLVSISVNGTPRGREKSQKLLMLFRE-- 723

Query: 723 DNEQQESYVSDPSSSNKAASNSDQRKPSKKS 725
             E+Q+    D  SSN+      Q++P++KS
Sbjct: 724 --ERQQ---RDQPSSNR--DEPPQKEPARKS 731

BLAST of Tan0008725 vs. TAIR 10
Match: AT1G24330.1 (ARM repeat superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 1.1e-78
Identity = 218/727 (29.99%), Postives = 363/727 (49.93%), Query Frame = 0

Query: 7   ENVEAVSNVYSFKVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLNIGKNKA 66
           EN+ A S+    K+H  MC EL  +  +V  I P +E ARP S  G + LC+L+I   KA
Sbjct: 8   ENLFAASDA---KLHGDMCKELSAVYCKVLSIFPSLEEARPRSKSGIQTLCSLHIALEKA 67

Query: 67  ELLLQYCRDSSKLYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQIADDLR 126
           + +LQ+C + SKLYL +TGD ++ +  + ++ L  +LR+++ +VP ++  +I  I  +L 
Sbjct: 68  KNILQHCSECSKLYLAITGDAVLLKFEKAKSALIDSLRRVEDIVPSSIGSQILDIVGELE 127

Query: 127 VAKFILDSSEEEVWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEVLFEKRS 186
             KF+LD SE+EV   +  LL+ G    +  +++E++    AA RL+I+S +  L E+R+
Sbjct: 128 HTKFLLDPSEKEVGDRIIALLQQGKKFDNGSDSTELEIFHQAATRLSITSSRSALAERRA 187

Query: 187 LRKLLDDVG-HGDPPKKKILTYLLYLLKKHGELIVQEITEARADISSS------NGYGEI 246
           L+K++D      D  K+ I+ YLL+L++K+ +L   E+ +      S+       G  E 
Sbjct: 188 LKKVIDRARVEEDKRKESIVAYLLHLMRKYSKLFRSEMMDENDSPCSTPCSPTGQGPNED 247

Query: 247 EVNVRQRNNASQADIIL---------NRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVW 306
            VN   R  +    I              IPPEE +CPIS++LMYDPV+IAS  TYE+V 
Sbjct: 248 RVNAFGRQLSKFGSINYKPMNSRKSGQMPIPPEELRCPISLQLMYDPVIIASGQTYERVC 307

Query: 307 IEKWFEEGHDTCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWEN 366
           IEKWF +GH++CP+T+ +L   S+TPN  +K LI  WC + G+T+P    E      W  
Sbjct: 308 IEKWFSDGHNSCPKTQQQLPHLSLTPNYCVKGLIASWCEQNGITVPTGPPESLDLNYWR- 367

Query: 367 SIASFGSSMNDIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGL-AIKYGQSKDDDLHRYQ 426
                  +M+D   P            NS   DS+ L     +  +   +S   +  R Q
Sbjct: 368 ------LAMSDSESP------------NSKSVDSVGLCTPKDIRVVPLEESSTIESERQQ 427

Query: 427 SDSNAEETNLEFPSTISELSWEL------------KCKVIKDMKNALNKNGVGPIL--SE 486
            + N     ++    + E   ++            KCKV+++++  L  N    IL  + 
Sbjct: 428 KEKNNAPDEVDSEINVLEGYQDILAIVDKEEDLAKKCKVVENVRILLKDNEEARILMGAN 487

Query: 487 TVMDQLALFLKDACDQQDSEAQKNGS-ELFLSLVRKNRSNSLNVPEKILTTLASLLN-SE 546
             ++    FL+ A    ++ AQ+ G+  LF   V  NR+  L +   ++  L  +++ S+
Sbjct: 488 GFVEAFLQFLESAVHDNNAAAQETGAMALFNLAVNNNRNKELMLTSGVIPLLEKMISCSQ 547

Query: 547 VTYEVLAILEAFSGHRKCSSNFVTSGVLASMVEYLDSEMKDLQEF-AIKTFSNLSSNSHI 606
                 A+    S   K      +S  ++  V  L  + K   +  A+    NLS+ S  
Sbjct: 548 SQGPATALYLNLSCLEKAKPVIGSSQAVSFFVNLLLQDTKTQCKLDALHALYNLSTYSPN 607

Query: 607 CSDIVSLGCIPKLVPLLN-DEALW-GKCIFILKNLCHTEEARIYIVETNGCIASIVQLLE 666
              ++S   I  L  L +    LW  K + +L NL  + E +  ++ T G I+++  +L+
Sbjct: 608 IPTLLSSNIIKSLQVLASTGNHLWIEKSLAVLLNLASSREGKEEMITTQGMISTLATVLD 667

Query: 667 MGSLEDQEHAVTILLSLCSQRVEYCELVMAEGVFPLLWNISNNGSEKGKAGAFELRRLLR 698
            G   +QE AV+ L+ LC+      ++V+ EGV P L +IS NGS +G+  + +L  L R
Sbjct: 668 TGDTVEQEQAVSCLVILCTGSESCIQMVLQEGVIPSLVSISVNGSPRGRDKSQKLLMLFR 712

BLAST of Tan0008725 vs. TAIR 10
Match: AT1G27910.1 (plant U-box 45 )

HSP 1 Score: 280.4 bits (716), Expect = 4.1e-75
Identity = 223/758 (29.42%), Postives = 382/758 (50.40%), Query Frame = 0

Query: 19  KVHCRMCTELMKLVDRVSEILPEIEAARPGSPEGREALCNLNIGKNKAELLLQYCRDSSK 78
           K+H +MC  L  +  ++  I P +EAARP S  G +ALC+L++   K + +L++C +SSK
Sbjct: 17  KLHGKMCNALSVIYCKIMSIFPSLEAARPRSKSGIQALCSLHVVLEKVKNILRHCTESSK 76

Query: 79  LYLTLTGDRIISRCHRVRTLLEHNLRKIKYMVPVALARKISQIADDLRVAKFILDSSEEE 138
           LYL +TGD ++ +  + ++ L  +LR+++ +V  ++  ++ +I  +L   +F LD +E+E
Sbjct: 77  LYLAITGDSVVLKFEKAKSSLTDSLRRVEDIVQQSIGSQLLEILMELENTEFSLDPAEKE 136

Query: 139 VWKAMRQLLKLGDSPPDALENSEIKALKIAALRLNISSYKEVLFEKRSLRKLLDDVG-HG 198
           +   +  LL+ G +   + +N+E++    AA RL I+S +  L E+R L+KL++      
Sbjct: 137 IGDQIIGLLQQGGNFESSSDNNELEVFHQAATRLGITSSRAALTERRCLKKLIERARMED 196

Query: 199 DPPKKKILTYLLYLLKKHGELIVQEITEARADISSS-------------NGYGEI----- 258
           D  K+ I+ YLL+L++K+ +L   EI +      SS             + +G       
Sbjct: 197 DKRKESIVAYLLHLMRKYSKLFRSEIWDDNDSQGSSSLPCSPTIQGSIDDAHGRAFDRQL 256

Query: 259 ----EVNVRQRNNASQADIILNRAIPPEEFKCPISMRLMYDPVVIASKVTYEKVWIEKWF 318
                 N R  NN  ++      ++PPEE +CPIS++LMYDPV+IAS  TYE++ IEKWF
Sbjct: 257 SKLSSFNFRSCNNNRRSS---QMSVPPEELRCPISLQLMYDPVIIASGQTYERICIEKWF 316

Query: 319 EEGHDTCPQTKMKLTDFSMTPNVDMKNLIDKWCVKFGVTIPDPSLEPECPEVWENSIASF 378
            +GH+TCP+T  +L+   +TPN  +K LI  WC + GV +PD   E      W   +A  
Sbjct: 317 SDGHNTCPKTHQQLSHLCLTPNYCVKALISSWCEQNGVQVPDGPPESLDLNYWR--LALS 376

Query: 379 GSSMNDIRLPIDFSNISFGGLDNSYYPDSLRLNGGNGLAI-KYGQSKDDDLH-RYQSDS- 438
            S   D R              ++    S +L     + + + G  K++     YQ D  
Sbjct: 377 VSESTDTR--------------SAKRVGSCKLKDVKVVPLEESGTIKEEACESEYQEDQV 436

Query: 439 NAEETNLEFPSTISEL-SWELKCKVIKDMKNALNKNGVGPIL--SETVMDQLALFLKDAC 498
              E   E  +T++++ +   KC+V++ ++  L  +    IL      ++ L  FL  A 
Sbjct: 437 TLVERCTELLTTLTDVDTLRKKCRVVEQIRVLLKDDEEARILMGENGCVEALLQFLGSAL 496

Query: 499 DQQDSEAQKNGS-ELFLSLVRKNRSNSLNVPEKILTTLASLL-NSEVTYEVLAILEAFSG 558
           ++ ++ AQK G+  LF   V  NR+  L +   I+  L  +L N      V AI    S 
Sbjct: 497 NENNASAQKVGAMALFNLAVDNNRNKELMLASGIIPLLEEMLCNPHSHGSVTAIYLNLSC 556

Query: 559 HRKCSSNFVTSGVLASMVEYLDSEMK-DLQEFAIKTFSNLSSNSHICSDIVSLGCIPKLV 618
             +      +S  +  MV  L +E +   +  A+ +  +LS+       ++S   +  L 
Sbjct: 557 LEEAKPVIGSSLAVPFMVNLLWTETEVQCKVDALHSLFHLSTYPPNIPCLLSADLVNALQ 616

Query: 619 PL-LNDEALW-GKCIFILKNLCHTEEARIYIVETNGCIASIVQLLEMGSLEDQEHAVTIL 678
            L ++DE  W  K + +L NL   E  +  +V     ++++  +L+ G   +QE AV++L
Sbjct: 617 SLTISDEQRWTEKSLAVLLNLVLNEAGKDEMVSAPSLVSNLCTILDTGEPNEQEQAVSLL 676

Query: 679 LSLCSQRVEYCELVMAEGVFPLLWNISNNGSEKGKAGAFELRRLLRDVQDNEQ------Q 733
           L LC+      E+V+ EGV P L +IS NG+++G+  A +L  L R+++  +Q      Q
Sbjct: 677 LILCNHSEICSEMVLQEGVIPSLVSISVNGTQRGRERAQKLLTLFRELRQRDQTHLTEPQ 736