Tan0007173 (gene) Snake gourd v1

Overview
NameTan0007173
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPhloem filament protein
LocationLG10: 57713210 .. 57713785 (+)
RNA-Seq ExpressionTan0007173
SyntenyTan0007173
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTAAATAGTATTAATCTTATCTTCTACCACTTTAATTTTCATTATTATCACATTAATTCAGTCCTATAAATATTGTATGAAATAAAGAAAGTCATGCCAAAACATATTTCCATAATATATCAAAGGGTAGGATGACTTTCGGATTTGGATTTGGCCTCAACTTTGGTTTGTTTGATCAAATGGTTCGTCCGCCACCAGGACACCATTGTCATGGAAAGTGGGTAGTGATTTCAAATCTCGACACGCCTTGCTTGCAACAAATTTCAAAATTTGCGGTGGAAGAACACAACAAACGACATAGGGAAAATTTTATATTCAAGCACCTTTATGGAGGTTGGTATTTGGAATTGGATCAGAACAGCCTGAAGTACCGTCTCCATATTGAAGTGACAAACTGTATTGGACGTGTGTCAAGGTTTGAGGCTATTGTGTTTGAAAAAAACACTGAATGCGGAAGAACCAGGACACTTGAGTCATTCAGACATATATAAAGAAGTGATCCAACTTCCTTTTAAATTTGTCATATCTATGTCACTTACGTTGTAATGGGTTTGATGATCTATCTATGTCACTTA

mRNA sequence

TCTAAATAGTATTAATCTTATCTTCTACCACTTTAATTTTCATTATTATCACATTAATTCAGTCCTATAAATATTGTATGAAATAAAGAAAGTCATGCCAAAACATATTTCCATAATATATCAAAGGGTAGGATGACTTTCGGATTTGGATTTGGCCTCAACTTTGGTTTGTTTGATCAAATGGTTCGTCCGCCACCAGGACACCATTGTCATGGAAAGTGGGTAGTGATTTCAAATCTCGACACGCCTTGCTTGCAACAAATTTCAAAATTTGCGGTGGAAGAACACAACAAACGACATAGGGAAAATTTTATATTCAAGCACCTTTATGGAGGTTGGTATTTGGAATTGGATCAGAACAGCCTGAAGTACCGTCTCCATATTGAAGTGACAAACTGTATTGGACGTGTGTCAAGGTTTGAGGCTATTGTGTTTGAAAAAAACACTGAATGCGGAAGAACCAGGACACTTGAGTCATTCAGACATATATAAAGAAGTGATCCAACTTCCTTTTAAATTTGTCATATCTATGTCACTTACGTTGTAATGGGTTTGATGATCTATCTATGTCACTTA

Coding sequence (CDS)

ATGACTTTCGGATTTGGATTTGGCCTCAACTTTGGTTTGTTTGATCAAATGGTTCGTCCGCCACCAGGACACCATTGTCATGGAAAGTGGGTAGTGATTTCAAATCTCGACACGCCTTGCTTGCAACAAATTTCAAAATTTGCGGTGGAAGAACACAACAAACGACATAGGGAAAATTTTATATTCAAGCACCTTTATGGAGGTTGGTATTTGGAATTGGATCAGAACAGCCTGAAGTACCGTCTCCATATTGAAGTGACAAACTGTATTGGACGTGTGTCAAGGTTTGAGGCTATTGTGTTTGAAAAAAACACTGAATGCGGAAGAACCAGGACACTTGAGTCATTCAGACATATATAA

Protein sequence

MTFGFGFGLNFGLFDQMVRPPPGHHCHGKWVVISNLDTPCLQQISKFAVEEHNKRHRENFIFKHLYGGWYLELDQNSLKYRLHIEVTNCIGRVSRFEAIVFEKNTECGRTRTLESFRHI
Homology
BLAST of Tan0007173 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 1.3e-04
Identity = 27/85 (31.76%), Postives = 47/85 (55.29%), Query Frame = 0

Query: 33  ISNLDTPCLQQISKFAVEEHNKRHRENFIFKHLYGGWYLELDQNSLKYRLHIEVTNCIGR 92
           I N+  P +  ++K+A+EEHNK  +E  +F  +  G    +  +  KY L I   +  G+
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVV--SGTKYDLKIAAKDGGGK 94

Query: 93  VSRFEAIVFEKNTECGRTRTLESFR 118
           +  +EA+V EK      +++LESF+
Sbjct: 95  IKNYEAVVVEKLWL--HSKSLESFK 115

BLAST of Tan0007173 vs. NCBI nr
Match: KAG6607543.1 (hypothetical protein SDJN03_00885, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 138.3 bits (347), Expect = 4.4e-29
Identity = 67/120 (55.83%), Postives = 83/120 (69.17%), Query Frame = 0

Query: 1   MTFGFGFGLNFGL-FDQMVRPPPGHHCHGKWVVISNLDTPCLQQISKFAVEEHNKRHREN 60
           M+ G G GL F L F    RP P   C+ KW+ I +L   CL Q++KFA+EEH+K HREN
Sbjct: 1   MSIGLGLGLGFNLGFPD--RPKPDCGCNEKWIPIQDLGMECLHQVAKFALEEHHKNHREN 60

Query: 61  FIFKHLYGGWYLELDQNSLKYRLHIEVTNCIGRVSRFEAIVFEKNTECGRTRTLESFRHI 120
           +  K + GGWYLE+D  S+KYRLHIEV++ +G V R E IV EK  ECGR R+LESFRHI
Sbjct: 61  WALKGISGGWYLEIDAYSVKYRLHIEVSSRLGFVRRHEVIVLEKRVECGRIRSLESFRHI 118

BLAST of Tan0007173 vs. NCBI nr
Match: KAG6607542.1 (hypothetical protein SDJN03_00884, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 124.4 bits (311), Expect = 6.6e-25
Identity = 51/86 (59.30%), Postives = 65/86 (75.58%), Query Frame = 0

Query: 1  MTFGFGFGLNFGLFDQMVRPPPGHHCHGKWVVISNLDTPCLQQISKFAVEEHNKRHRENF 60
          MT G   G+N G FD   RP  GHHCHGKWV I NLD+PC+Q+IS+FAVEE+N R+R+N 
Sbjct: 1  MTIGLNLGINLGFFDHHGRPRLGHHCHGKWVEIPNLDSPCMQEISRFAVEEYNHRYRKNL 60

Query: 61 IFKHLYGGWYLELDQNSLKYRLHIEV 87
           ++ +Y  WYLE+D  S+KYRLHI+V
Sbjct: 61 TYQFIYAAWYLEVDARSVKYRLHIQV 86

BLAST of Tan0007173 vs. NCBI nr
Match: KAG6588756.1 (hypothetical protein SDJN03_17321, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 93.2 bits (230), Expect = 1.6e-15
Identity = 39/94 (41.49%), Postives = 61/94 (64.89%), Query Frame = 0

Query: 26  CHGKWVVISNLDTPCLQQISKFAVEEHNKRHRENFIFKHLYGGWYLELDQNSLKYRLHIE 85
           C  KW+ I N+   C+Q ++KFAVE++N  H+E+F +K++  GW++EL    L +RLH+E
Sbjct: 23  CPSKWIPIPNVSEICVQNVAKFAVEDYNLEHKESFKYKNIKCGWFMELKGEDLAFRLHVE 82

Query: 86  VTNCIGRVSRFEAIVFEKNTECGRTRTLESFRHI 120
           V +C+GRV   + +V EK     + R LES + I
Sbjct: 83  VKDCLGRVFEMDVVVSEKKVCAEKVRKLESAKLI 116

BLAST of Tan0007173 vs. NCBI nr
Match: XP_023530188.1 (uncharacterized protein LOC111792827 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 92.8 bits (229), Expect = 2.1e-15
Identity = 42/88 (47.73%), Postives = 60/88 (68.18%), Query Frame = 0

Query: 29  KWVVISNLDTPCLQQISKFAVEEHNKRHRENFIFKHLYGGWYLELDQNSLKYRLHIEVTN 88
           KW+ I ++   CLQQ++KFAVE+ N  H +  IF  ++ GWYLEL  N+LKYRLHI+  +
Sbjct: 16  KWIKIPDVKGHCLQQVAKFAVEKFNAEHGDCLIFDSIFEGWYLELGSNNLKYRLHIKAID 75

Query: 89  CIGRVSRFEAIVFEKNTECGRTRTLESF 117
            +GR+  +EA+VFE+  +  R R L SF
Sbjct: 76  FLGRLLIYEAVVFEEKPDKERIRKLISF 103

BLAST of Tan0007173 vs. NCBI nr
Match: KAG6588758.1 (hypothetical protein SDJN03_17323, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 92.4 bits (228), Expect = 2.8e-15
Identity = 41/94 (43.62%), Postives = 60/94 (63.83%), Query Frame = 0

Query: 26  CHGKWVVISNLDTPCLQQISKFAVEEHNKRHRENFIFKHLYGGWYLELDQNSLKYRLHIE 85
           C  KW+ I ++   C+Q I+KFAVE++N  H+E+F +K +  GW++EL    L YRLHIE
Sbjct: 24  CPSKWIQIPDVSEVCVQNIAKFAVEDYNLVHKESFKYKSIKCGWFMELKGEDLAYRLHIE 83

Query: 86  VTNCIGRVSRFEAIVFEKNTECGRTRTLESFRHI 120
           V +C+GR+     +V EK T   + R LES + I
Sbjct: 84  VKDCLGRIFELNVVVSEKKTCAEKIRKLESAKLI 117

BLAST of Tan0007173 vs. ExPASy TrEMBL
Match: A0A6J1EIQ8 (uncharacterized protein LOC111434517 OS=Cucurbita moschata OX=3662 GN=LOC111434517 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.3e-15
Identity = 40/88 (45.45%), Postives = 60/88 (68.18%), Query Frame = 0

Query: 29  KWVVISNLDTPCLQQISKFAVEEHNKRHRENFIFKHLYGGWYLELDQNSLKYRLHIEVTN 88
           KW+ I +++  CLQQ++KFAVE+ N  H +  I+  ++ GWYLEL  N+LKYRLHI+  +
Sbjct: 16  KWIKIPDVNGHCLQQVAKFAVEKFNAEHGDCLIYNSIFEGWYLELGSNNLKYRLHIKAVD 75

Query: 89  CIGRVSRFEAIVFEKNTECGRTRTLESF 117
            +GR+  +E +VFE+  +  R R L SF
Sbjct: 76  FLGRLLIYEVVVFEEKPDKERIRKLISF 103

BLAST of Tan0007173 vs. ExPASy TrEMBL
Match: A0A6J1JKF7 (uncharacterized protein LOC111486626 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486626 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.3e-14
Identity = 43/113 (38.05%), Postives = 69/113 (61.06%), Query Frame = 0

Query: 5   FGFGLNFGLFDQMVRPPPGHHCHGKWVVISNLDTPCLQQISKFAVEEHNKRHRENFIFKH 64
           F + LN G +   V PP       KW+ I +L  P +Q++SKFA++E+NK   E   +  
Sbjct: 103 FLYLLNGGHYVGPVDPPQVE----KWIKIPDLRVPFVQEVSKFAIDEYNK-GGEGLKYVE 162

Query: 65  LYGGWYLELDQNSLKYRLHIEVTNCIGRVSRFEAIVFEKNTECGRTRTLESFR 118
           +Y GWY E+ Q+++K+RLH++  +C+GR+  +EAIV  K     + + LESF+
Sbjct: 163 IYDGWYAEMGQDNIKFRLHVKAKDCLGRLRNYEAIVLVKQFLSKKIKILESFK 210

BLAST of Tan0007173 vs. ExPASy TrEMBL
Match: A0A6J1JG70 (uncharacterized protein LOC111486626 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486626 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.3e-14
Identity = 43/113 (38.05%), Postives = 69/113 (61.06%), Query Frame = 0

Query: 5   FGFGLNFGLFDQMVRPPPGHHCHGKWVVISNLDTPCLQQISKFAVEEHNKRHRENFIFKH 64
           F + LN G +   V PP       KW+ I +L  P +Q++SKFA++E+NK   E   +  
Sbjct: 103 FLYLLNGGHYVGPVDPPQVE----KWIKIPDLRVPFVQEVSKFAIDEYNK-GGEGLKYVE 162

Query: 65  LYGGWYLELDQNSLKYRLHIEVTNCIGRVSRFEAIVFEKNTECGRTRTLESFR 118
           +Y GWY E+ Q+++K+RLH++  +C+GR+  +EAIV  K     + + LESF+
Sbjct: 163 IYDGWYAEMGQDNIKFRLHVKAKDCLGRLRNYEAIVLVKQFLSKKIKILESFK 210

BLAST of Tan0007173 vs. ExPASy TrEMBL
Match: P94012 (Phloem filament protein OS=Cucurbita maxima OX=3661 GN=PP1 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.3e-14
Identity = 43/113 (38.05%), Postives = 69/113 (61.06%), Query Frame = 0

Query: 5   FGFGLNFGLFDQMVRPPPGHHCHGKWVVISNLDTPCLQQISKFAVEEHNKRHRENFIFKH 64
           F + LN G +   V PP       KW+ I +L  P +Q++SKFA++E+NK   E   +  
Sbjct: 103 FLYLLNGGHYVGPVDPPQVE----KWIKIPDLRVPFVQEVSKFAIDEYNK-GGEGLKYVE 162

Query: 65  LYGGWYLELDQNSLKYRLHIEVTNCIGRVSRFEAIVFEKNTECGRTRTLESFR 118
           +Y GWY E+ Q+++K+RLH++  +C+GR+  +EAIV  K     + + LESF+
Sbjct: 163 IYDGWYAEMGQDNIKFRLHVKAKDCLGRLRNYEAIVLVKQFLSKKIKILESFK 210

BLAST of Tan0007173 vs. ExPASy TrEMBL
Match: A0A0A0K471 (Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G067340 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 7.3e-14
Identity = 39/98 (39.80%), Postives = 63/98 (64.29%), Query Frame = 0

Query: 26  CHGKWVVISNLDTPCLQQISKFAVEEHNKRHRENFIFKHLYGGWYLELDQ--NSLKYRLH 85
           C  +W+   N+  PCLQ I++  +EE  K+H  + I+K++  GWY ELD   N+++YRLH
Sbjct: 8   CSKRWIKFPNVLIPCLQAIAEHGIEEFKKKHEVSLIYKNVVEGWYKELDDHGNTIRYRLH 67

Query: 86  IEVTNCIGRVSRFEAIVFEKNTECGR--TRTLESFRHI 120
           I+  +CI R+ +FEA++ +++ +     T TLESF  I
Sbjct: 68  IQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI 105

BLAST of Tan0007173 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 9.5e-06
Identity = 27/85 (31.76%), Postives = 47/85 (55.29%), Query Frame = 0

Query: 33  ISNLDTPCLQQISKFAVEEHNKRHRENFIFKHLYGGWYLELDQNSLKYRLHIEVTNCIGR 92
           I N+  P +  ++K+A+EEHNK  +E  +F  +  G    +  +  KY L I   +  G+
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVV--SGTKYDLKIAAKDGGGK 94

Query: 93  VSRFEAIVFEKNTECGRTRTLESFR 118
           +  +EA+V EK      +++LESF+
Sbjct: 95  IKNYEAVVVEKLWL--HSKSLESFK 115

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84WT81.3e-0431.76Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
KAG6607543.14.4e-2955.83hypothetical protein SDJN03_00885, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6607542.16.6e-2559.30hypothetical protein SDJN03_00884, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6588756.11.6e-1541.49hypothetical protein SDJN03_17321, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023530188.12.1e-1547.73uncharacterized protein LOC111792827 [Cucurbita pepo subsp. pepo][more]
KAG6588758.12.8e-1543.62hypothetical protein SDJN03_17323, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1EIQ82.3e-1545.45uncharacterized protein LOC111434517 OS=Cucurbita moschata OX=3662 GN=LOC1114345... [more]
A0A6J1JKF77.3e-1438.05uncharacterized protein LOC111486626 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JG707.3e-1438.05uncharacterized protein LOC111486626 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
P940127.3e-1438.05Phloem filament protein OS=Cucurbita maxima OX=3661 GN=PP1 PE=4 SV=1[more]
A0A0A0K4717.3e-1439.80Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G067340 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G16500.19.5e-0631.76Cystatin/monellin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009994Phloem filament PP1PFAMPF07430PP1coord: 39..116
e-value: 3.4E-24
score: 84.7
NoneNo IPR availableGENE3D3.10.450.10coord: 23..119
e-value: 1.8E-13
score: 52.5
NoneNo IPR availablePANTHERPTHR47116:SF5PHLOEM FILAMENT PROTEINcoord: 27..119
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 28..118
IPR027214CystatinPANTHERPTHR47116PHLOEM FILAMENT PROTEINcoord: 27..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007173.1Tan0007173.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity