Tan0005969 (gene) Snake gourd v1

Overview
NameTan0005969
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionIAA-amino acid hydrolase ILR1-like 1
LocationLG04: 11027226 .. 11030175 (+)
RNA-Seq ExpressionTan0005969
SyntenyTan0005969
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAGCCATATGGAGCTTCTGTACTTCGTCATCATCGCCTCCATTTTTGTGCCTTTCAGCTTCTCTCTCAAGCTTGAAACTATCGTCCATGGAACAACTCCGAGTTCCATTCAAACCAATCAAGATTCTTCACTCACACACCTAATTCAGGAGCTCGCCAATGATCCCACCACTTCAAATTGGATGAAGACGATAAGGAGAAAAATCCACGAGAACCCAGAGCTTGCTTATGAAGAATTTGAAACCAGTAGGCTTATTAGACAGGAGCTTGACAATCTCGGCGTTTCTTATCGATGGCCGGTCGCCGGTACCGGCCTAGTGGCTTCGGTCGGTTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCAGATATGGATGCTTTACCTATCGAGGTCAGATTTGGTTCATTTCATATTTCTCATTCCCCCGCCATTAAATGCTATCTTCTGCTCGATGAAATGACCAAATGAACTTGATTGGTTCTTCTTCTTCTCCATTTTTGGATCCGGCTTGATGGGAATCTGAAGTTTGTTCTCATCCGTTTAATCGTAATTTTTTAAACATATAACCGACAAAACTATTTCATTGATGATAATGCAGAACCAAAGTCTAAAAAAGAACTAATTATGTCTAAAACATGACAGAGTTCTTAATTTAACAAACAATTTTTAAAATAATCTTGAAGTGCAAGAAGATAGCAAACCTTTTTTTCGATTAGGGAATATGGTTTCAGATGTACCAGAGTCTGTGCAATAATTAATGACCAGCCATTGAAATCATAGAATTTCTTAAACTATCTTTCTTTACTTTGGAACTGGACCATATAAGATGGGTGGAAATCTAAAATGAAAGCACTTTTGTTTTGCTTAATGGATTTTGATATAGGAATTGGTGGAATGGGAGCATAAGAGCAAAGTGAAGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTAGCTATGCTTCTTGGCGCTACTAAGATTCTGCATCAACTGCGGCAGAAATTACAGGTTCTCAAAGTACATTGCAAGAACTCCCCAGTTCCAATAACTTTCCGATTGATTTGTTTAGAAAGAAGATGATTATGGCTCAAACTTGTATCCTCTTTTCTTAATATGAACATGTTCTCTTCAATGCAGGGCACTGTGGTTCTTATATTTCAGCCAGCCGAGGAGAGGGGAGGAGGAGCAAAAGACATGATAAAAGAAGGTGTCCTTGATGGTGTAGAAGCTATATTGGGGCTGCACATCATGCATAAATATCCGGTGGGTGTTGTCGCTTCACGTCCCGGCGAGTTTCTTGCGGGGTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGCCATGCAGCAATTCCACAGCATTCAATTGATCCTGTTTTGGCAGCTTCAGCAGCAATTGTTAGTCTGCAAAACATAGTCTCAAGAGAGATAGACCCTTTAGATTCCCAGGTACTTTCTTTCAGATGAACATAGATGATAAAAGTCTCAATGGATTCAATTCATCCTTTTGTAACTCTCTTGGCGGATCTGATCATACAAACAAGACCGTCTGTTTCTTTTCATCTTAAAGGAGAGTGTTGAAAGTTGCAGGTGGTTTCTGTGGCCATGGTTCAAGCAGGAACTGCACTTAATGTCATCCCGGACTCGGCCACCATTGCTGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCTCTCAGAGATAGGATAGAAGAGGTAACCCCAAGAACAGGTTCTTCTACAGAACAGATGAATTGTCACTGCACCTTGTGACTGAAATTTTATGTTCACTTCTTGTGGCAGGTCATAAATGGGCAGGCAGCAGTACACCGGTGCACAGCGGAGATTGATTTCCTAGGAAAGGAACACCCGACGGTTCCTCCGACTATAAATAACGAGAAAATTTACGAACATGCTCGACGTGTCTCTATCGAAATTGTAGGAAAAGAGAACACAGAAGTATCTCCAAACTTAATGGGAAGTGAAGATTTTGCTTTCTTTTTGGACCGAGTGCCGGGATCTTTCTTACTTCTAGGCACATATAATGAGAGGATTGGAGCTATATATCCTCCACACAGCCCTCGCTATAAAATTGATGAAAATGTGCTTCCTATTGGCGCAGCAATTCATGCAGCATTTGCATATTCTTATCTCTTGAATTCATCATCAACATCTCAGTTTCCCTGTTCTACAATCTTGAATAGGGAAAGTCCTTAAGTTATAGTCCTATCAAAACATCTCGTTTAAGAAACTCAAGTTCATCGATATTTTGAAACCTCTTCATGGGGCAAAAGAGAGTGGCATAGCATAAAATATGATTGTTCTTACTTATTACAAAAGAGTTTTGCTTTCAGTTTCTTTAGCTGATGTTGTTCCAAACAAGTAGTCTGGATTGTGGAATATTATTACAACATGTCAAAGGAAAACCAATAAACAAAGCCTGATTAAAATCAGGCGAAGAAGAGAAGGATAACAACATGATCTATAGAGAGAGATCAAGCAACACCAAAAAGACAACAGAAAAGAAAGAGAGCTATGGAATACATAACATCTCAGATTCTTTCCCGGACGATGAACATGGTAAGAAGGATAAACATGAGTCCAAGCACCAACGACGGACTGGTAAAGCTGCCGTAGGAGCCGAGCGACGTCGGAGGTGAAGGGTTCGGGGGCATGTACATGCCAGGCATAGAATCATCTGCTGCAACTAATGTGAGGAGAAGAGCAAAATAGGCTGTGAATATGAGCTTATTAGCCATATGTAGAAGAAGTGTGTTGAAGTGGACTTATGAGAGGGATAAATTTTCTAAGTTTGTTATTGTTGCTTATTTCTTAGTGTAACCAAATGGATGGAGGTGTATATATATGAGTGAAAATTTGGTGATTAAAGTTCTTAGAAGTTATAACTTTCTGTAATAACTTCTGTTTTTCGTTATAGCCGAG

mRNA sequence

CTCAGCCATATGGAGCTTCTGTACTTCGTCATCATCGCCTCCATTTTTGTGCCTTTCAGCTTCTCTCTCAAGCTTGAAACTATCGTCCATGGAACAACTCCGAGTTCCATTCAAACCAATCAAGATTCTTCACTCACACACCTAATTCAGGAGCTCGCCAATGATCCCACCACTTCAAATTGGATGAAGACGATAAGGAGAAAAATCCACGAGAACCCAGAGCTTGCTTATGAAGAATTTGAAACCAGTAGGCTTATTAGACAGGAGCTTGACAATCTCGGCGTTTCTTATCGATGGCCGGTCGCCGGTACCGGCCTAGTGGCTTCGGTCGGTTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCAGATATGGATGCTTTACCTATCGAGGAATTGGTGGAATGGGAGCATAAGAGCAAAGTGAAGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTAGCTATGCTTCTTGGCGCTACTAAGATTCTGCATCAACTGCGGCAGAAATTACAGGGCACTGTGGTTCTTATATTTCAGCCAGCCGAGGAGAGGGGAGGAGGAGCAAAAGACATGATAAAAGAAGGTGTCCTTGATGGTGTAGAAGCTATATTGGGGCTGCACATCATGCATAAATATCCGGTGGGTGTTGTCGCTTCACGTCCCGGCGAGTTTCTTGCGGGGTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGCCATGCAGCAATTCCACAGCATTCAATTGATCCTGTTTTGGCAGCTTCAGCAGCAATTGTTAGTCTGCAAAACATAGTCTCAAGAGAGATAGACCCTTTAGATTCCCAGGTGGTTTCTGTGGCCATGGTTCAAGCAGGAACTGCACTTAATGTCATCCCGGACTCGGCCACCATTGCTGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCTCTCAGAGATAGGATAGAAGAGGTCATAAATGGGCAGGCAGCAGTACACCGGTGCACAGCGGAGATTGATTTCCTAGGAAAGGAACACCCGACGGTTCCTCCGACTATAAATAACGAGAAAATTTACGAACATGCTCGACGTGTCTCTATCGAAATTGTAGGAAAAGAGAACACAGAAGTATCTCCAAACTTAATGGGAAGTGAAGATTTTGCTTTCTTTTTGGACCGAGTGCCGGGATCTTTCTTACTTCTAGGCACATATAATGAGAGGATTGGAGCTATATATCCTCCACACAGCCCTCGCTATAAAATTGATGAAAATGTGCTTCCTATTGGCGCAGCAATTCATGCAGCATTTGCATATTCTTATCTCTTGAATTCATCATCAACATCTCAGTTTCCCTGTTCTACAATCTTGAATAGGGAAAGTCCTTAAGTTATAGTCCTATCAAAACATCTCGTTTAAGAAACTCAAGTTCATCGATATTTTGAAACCTCTTCATGGGGCAAAAGAGAGTGGCATAGCATAAAATATGATTGTTCTTACTTATTACAAAAGAGTTTTGCTTTCAGTTTCTTTAGCTGATGTTGTTCCAAACAAGTAGTCTGGATTGTGGAATATTATTACAACATGTCAAAGGAAAACCAATAAACAAAGCCTGATTAAAATCAGGCGAAGAAGAGAAGGATAACAACATGATCTATAGAGAGAGATCAAGCAACACCAAAAAGACAACAGAAAAGAAAGAGAGCTATGGAATACATAACATCTCAGATTCTTTCCCGGACGATGAACATGGTAAGAAGGATAAACATGAGTCCAAGCACCAACGACGGACTGGTAAAGCTGCCGTAGGAGCCGAGCGACGTCGGAGGTGAAGGGTTCGGGGGCATGTACATGCCAGGCATAGAATCATCTGCTGCAACTAATGTGAGGAGAAGAGCAAAATAGGCTGTGAATATGAGCTTATTAGCCATATGTAGAAGAAGTGTGTTGAAGTGGACTTATGAGAGGGATAAATTTTCTAAGTTTGTTATTGTTGCTTATTTCTTAGTGTAACCAAATGGATGGAGGTGTATATATATGAGTGAAAATTTGGTGATTAAAGTTCTTAGAAGTTATAACTTTCTGTAATAACTTCTGTTTTTCGTTATAGCCGAG

Coding sequence (CDS)

ATGGAGCTTCTGTACTTCGTCATCATCGCCTCCATTTTTGTGCCTTTCAGCTTCTCTCTCAAGCTTGAAACTATCGTCCATGGAACAACTCCGAGTTCCATTCAAACCAATCAAGATTCTTCACTCACACACCTAATTCAGGAGCTCGCCAATGATCCCACCACTTCAAATTGGATGAAGACGATAAGGAGAAAAATCCACGAGAACCCAGAGCTTGCTTATGAAGAATTTGAAACCAGTAGGCTTATTAGACAGGAGCTTGACAATCTCGGCGTTTCTTATCGATGGCCGGTCGCCGGTACCGGCCTAGTGGCTTCGGTCGGTTCTGGTTCTCCTCCCTTTGTCGCTCTAAGAGCAGATATGGATGCTTTACCTATCGAGGAATTGGTGGAATGGGAGCATAAGAGCAAAGTGAAGGGGAAAATGCATGCTTGTAGTCATGATGCTCATGTAGCTATGCTTCTTGGCGCTACTAAGATTCTGCATCAACTGCGGCAGAAATTACAGGGCACTGTGGTTCTTATATTTCAGCCAGCCGAGGAGAGGGGAGGAGGAGCAAAAGACATGATAAAAGAAGGTGTCCTTGATGGTGTAGAAGCTATATTGGGGCTGCACATCATGCATAAATATCCGGTGGGTGTTGTCGCTTCACGTCCCGGCGAGTTTCTTGCGGGGTGTGGAAGCTTCAAAGCAAAGATAAAGGGGAAAGGGGGCCATGCAGCAATTCCACAGCATTCAATTGATCCTGTTTTGGCAGCTTCAGCAGCAATTGTTAGTCTGCAAAACATAGTCTCAAGAGAGATAGACCCTTTAGATTCCCAGGTGGTTTCTGTGGCCATGGTTCAAGCAGGAACTGCACTTAATGTCATCCCGGACTCGGCCACCATTGCTGGTACCTTCAGAGCTTTCAGCAAGAAGAGCTTCAATGCTCTCAGAGATAGGATAGAAGAGGTCATAAATGGGCAGGCAGCAGTACACCGGTGCACAGCGGAGATTGATTTCCTAGGAAAGGAACACCCGACGGTTCCTCCGACTATAAATAACGAGAAAATTTACGAACATGCTCGACGTGTCTCTATCGAAATTGTAGGAAAAGAGAACACAGAAGTATCTCCAAACTTAATGGGAAGTGAAGATTTTGCTTTCTTTTTGGACCGAGTGCCGGGATCTTTCTTACTTCTAGGCACATATAATGAGAGGATTGGAGCTATATATCCTCCACACAGCCCTCGCTATAAAATTGATGAAAATGTGCTTCCTATTGGCGCAGCAATTCATGCAGCATTTGCATATTCTTATCTCTTGAATTCATCATCAACATCTCAGTTTCCCTGTTCTACAATCTTGAATAGGGAAAGTCCTTAA

Protein sequence

MELLYFVIIASIFVPFSFSLKLETIVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWMKTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRADMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTFRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVSIEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVLPIGAAIHAAFAYSYLLNSSSTSQFPCSTILNRESP
Homology
BLAST of Tan0005969 vs. ExPASy Swiss-Prot
Match: Q84XG9 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 7.1e-131
Identity = 233/387 (60.21%), Postives = 288/387 (74.42%), Query Frame = 0

Query: 50  ANDPTTSNWMKTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGS 109
           A +   + WM  +RR+IHENPEL YEEF TS L+R+ELD LG+ YR P A TG+VA+VG+
Sbjct: 40  AKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVATVGT 99

Query: 110 GSPPFVALRADMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQ 169
           G PPFVALRADMDALP++E VEWEHKSKV GKMH C HDAHVAMLLG+ +IL + R +L+
Sbjct: 100 GGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHRDELK 159

Query: 170 GTVVLIFQPAEERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSF 229
           GTVVL+FQPAEE GGGAK MI +G ++ +EAI G+H+    P+GVVASRPG  +AG G F
Sbjct: 160 GTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPVMAGSGFF 219

Query: 230 KAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNV 289
           +A I GKGGHAA+P H+IDP+LAAS  IVSLQ +VSRE DPLDSQVV+V   Q G A NV
Sbjct: 220 EAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGGAFNV 279

Query: 290 IPDSATIAGTFRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNE 349
           IPDS TI GTFRAF K+SFN L+ RIEEVI  QA+V RC A +DFL K+ P  PPTIN+ 
Sbjct: 280 IPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPTINSA 339

Query: 350 KIYEHARRVSIEIVGKENTEVSPNLMGSEDFAFFLDRVPGS-FLLLGTYNERIGAIYPPH 409
            +++   +V+ E+VG +N      LMG+EDFAF+ D +P + +  LG YNE  G   P H
Sbjct: 340 GLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQAPHH 399

Query: 410 SPRYKIDENVLPIGAAIHAAFAYSYLL 436
           SP + I+E+ LP GAA+ A+ A  YLL
Sbjct: 400 SPYFTINEDALPYGAALQASLATRYLL 426

BLAST of Tan0005969 vs. ExPASy Swiss-Prot
Match: Q8S9S4 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 7.1e-131
Identity = 233/387 (60.21%), Postives = 288/387 (74.42%), Query Frame = 0

Query: 50  ANDPTTSNWMKTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGS 109
           A +   + WM  +RR+IHENPEL YEEF TS L+R+ELD LG+ YR P A TG+VA+VG+
Sbjct: 40  AKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTGVVATVGT 99

Query: 110 GSPPFVALRADMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQ 169
           G PPFVALRADMDALP++E VEWEHKSKV GKMH C HDAHVAMLLG+ +IL + R +L+
Sbjct: 100 GGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQEHRDELK 159

Query: 170 GTVVLIFQPAEERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSF 229
           GTVVL+FQPAEE GGGAK MI +G ++ +EAI G+H+    P+GVVASRPG  +AG G F
Sbjct: 160 GTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPVMAGSGFF 219

Query: 230 KAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNV 289
           +A I GKGGHAA+P H+IDP+LAAS  IVSLQ +VSRE DPLDSQVV+V   Q G A NV
Sbjct: 220 EAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQGGGAFNV 279

Query: 290 IPDSATIAGTFRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNE 349
           IPDS TI GTFRAF K+SFN L+ RIEEVI  QA+V RC A +DFL K+ P  PPTIN+ 
Sbjct: 280 IPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFFPPTINSA 339

Query: 350 KIYEHARRVSIEIVGKENTEVSPNLMGSEDFAFFLDRVPGS-FLLLGTYNERIGAIYPPH 409
            +++   +V+ E+VG +N      LMG+EDFAF+ D +P + +  LG YNE  G   P H
Sbjct: 340 GLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETRGPQAPHH 399

Query: 410 SPRYKIDENVLPIGAAIHAAFAYSYLL 436
           SP + I+E+ LP GAA+ A+ A  YLL
Sbjct: 400 SPYFTINEDALPYGAALQASLAARYLL 426

BLAST of Tan0005969 vs. ExPASy Swiss-Prot
Match: O04373 (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=1 SV=2)

HSP 1 Score: 456.4 bits (1173), Expect = 3.6e-127
Identity = 235/431 (54.52%), Postives = 306/431 (71.00%), Query Frame = 0

Query: 11  SIFVPFSFSLKLETIVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWMKTIRRKIHENP 70
           S F   SF L L  +    T  S  +N  S +      LA      +WM  IRR+IHENP
Sbjct: 2   SFFKWVSFVLILHLL--NPTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIHENP 61

Query: 71  ELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRADMDALPIEELV 130
           EL YEE ETS+L+R EL+ +GVSY++PVA TG+V  VG+G  PFVALRADMDAL ++E+V
Sbjct: 62  ELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMV 121

Query: 131 EWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMI 190
           EWEHKSKV GKMHAC HDAH  MLLGA K+L +  ++LQGTVVL+FQPAEE GGGAK ++
Sbjct: 122 EWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIV 181

Query: 191 KEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPV 250
           + GVL+ V AI GLH+ ++  +G V+SR G  LAG G FKAKI GKGGHAA+PQH+IDP+
Sbjct: 182 EAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPI 241

Query: 251 LAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTFRAFSKKSFNA 310
           LAAS  IVSLQ++VSRE DPLDSQVV+VA  + G A NVIPDS TI GTFRAFS KSF  
Sbjct: 242 LAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQ 301

Query: 311 LRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVSIEIVGKENTEV 370
           L+ RIE+VI  QA+V+ C A +DF+ +E P  PPT+N++ +++  + VS +++G EN   
Sbjct: 302 LKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVE 361

Query: 371 SPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVLPIGAAIHAAFA 430
              LMGSEDF+F+   +PG F  +G  N+    +  PHSP ++++E +LP GA++HA+ A
Sbjct: 362 MQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMA 421

Query: 431 YSYLLNSSSTS 442
             YLL   +++
Sbjct: 422 TRYLLELKAST 430

BLAST of Tan0005969 vs. ExPASy Swiss-Prot
Match: P54970 (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=1 SV=2)

HSP 1 Score: 451.8 bits (1161), Expect = 9.0e-126
Identity = 229/400 (57.25%), Postives = 287/400 (71.75%), Query Frame = 0

Query: 40  SSLTHLIQELANDPTTSNWMKTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVA 99
           S +   + E A  P   +WM  IRRKIHENPEL YEE ETS+LIR EL+ +G+ YR+PVA
Sbjct: 32  SQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSKLIRSELELIGIKYRYPVA 91

Query: 100 GTGLVASVGSGSPPFVALRADMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATK 159
            TG++  +G+G PPFVALRADMDALPI+E VEWEHKSK+ GKMHAC HD HV MLLGA K
Sbjct: 92  ITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGKMHACGHDGHVTMLLGAAK 151

Query: 160 ILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRP 219
           ILH+ R  LQGTVVLIFQPAEE   GAK M +EG L  VEAI G+H+  + P G  ASR 
Sbjct: 152 ILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAIFGIHLSARIPFGKAASRA 211

Query: 220 GEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVA 279
           G FLAG G F+A I GKGGHAAIPQH+IDPV+AAS+ ++SLQ +VSRE DPLDS+VV+V+
Sbjct: 212 GSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQQLVSRETDPLDSKVVTVS 271

Query: 280 MVQAGTALNVIPDSATIAGTFRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEH 339
            V  G A NVIPDS TI GT RAF+   F  L+ R++EVI  QAAVHRC A ++      
Sbjct: 272 KVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQRVKEVITKQAAVHRCNASVNLTPNGR 331

Query: 340 PTVPPTINNEKIYEHARRVSIEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNE 399
             +PPT+NN+ +Y+  ++V  +++G+E    +  +MGSEDF++F + +PG F LLG  +E
Sbjct: 332 EPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQDE 391

Query: 400 RIGAIYPPHSPRYKIDENVLPIGAAIHAAFAYSYLLNSSS 440
             G     HSP Y+I+E+VLP GAAIHA+ A  YL   +S
Sbjct: 392 TNG-YASSHSPLYRINEDVLPYGAAIHASMAVQYLKEKAS 428

BLAST of Tan0005969 vs. ExPASy Swiss-Prot
Match: P54969 (IAA-amino acid hydrolase ILR1-like 1 OS=Arabidopsis thaliana OX=3702 GN=ILL1 PE=1 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.9e-123
Identity = 229/392 (58.42%), Postives = 283/392 (72.19%), Query Frame = 0

Query: 48  ELANDPTTSNWMKTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASV 107
           ELA  P   + M  IRRKIHENPEL YEEFETS+ IR ELD +GV YR+PVA TG++  +
Sbjct: 39  ELAKSPEVFDSMVRIRRKIHENPELGYEEFETSKFIRSELDLIGVKYRFPVAITGIIGYI 98

Query: 108 GSGSPPFVALRADMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQK 167
           G+G PPFVALRADMDALPI+E VEWEHKSK  GKMHAC HD HVAMLLGA KIL Q RQ 
Sbjct: 99  GTGEPPFVALRADMDALPIQEAVEWEHKSKNPGKMHACGHDGHVAMLLGAAKILQQHRQH 158

Query: 168 LQGTVVLIFQPAEERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCG 227
           LQGTVVLIFQPAEE   GAK M +EG L  VEAI G+H+  + P G  AS  G F+AG G
Sbjct: 159 LQGTVVLIFQPAEEGLSGAKMMREEGALKNVEAIFGIHLSPRTPFGKAASLAGSFMAGAG 218

Query: 228 SFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTAL 287
           +F+A I GKGGHAAIPQH+IDPV+AAS+ ++SLQ++VSRE DP DS+VV+V  V  G A 
Sbjct: 219 AFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQHLVSRETDPSDSKVVTVTKVNGGNAF 278

Query: 288 NVIPDSATIAGTFRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTIN 347
           NVIPDS TI GT RAF+   F  L++RI+E+I  QAAVHRC A ++     +  +PPT+N
Sbjct: 279 NVIPDSITIGGTLRAFT--GFTQLQERIKEIITKQAAVHRCNASVNLAPNGNQPMPPTVN 338

Query: 348 NEKIYEHARRVSIEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPP 407
           N  +Y+  ++V  +++G+E    +   MGSEDF++F + +PG F LLG  +E  G     
Sbjct: 339 NMDLYKKFKKVVRDLLGQEAFVEAVPEMGSEDFSYFAETIPGHFSLLGMQDETQG-YASS 398

Query: 408 HSPRYKIDENVLPIGAAIHAAFAYSYLLNSSS 440
           HSP Y+I+E+VLP GAAIHA  A  YL + +S
Sbjct: 399 HSPHYRINEDVLPYGAAIHATMAVQYLKDKAS 427

BLAST of Tan0005969 vs. NCBI nr
Match: XP_022941713.1 (IAA-amino acid hydrolase ILR1-like 1 [Cucurbita moschata] >KAG6600138.1 IAA-amino acid hydrolase ILR1-like 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030803.1 IAA-amino acid hydrolase ILR1-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 795.4 bits (2053), Expect = 2.5e-226
Identity = 401/441 (90.93%), Postives = 417/441 (94.56%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLETIVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWMK 60
           MELLYF+II SIF       KLE  VHGTT SS+QT+Q+SSL H I ELANDPTTSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTTASSVQTSQNSSLRHQILELANDPTTSNWMK 60

Query: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTG+VASVGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGVE ILGLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDS TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVSI 360
           RAF KKSFNALRDRIEE+INGQAAVHRCT EIDFLGKEHPT+PPTINNEK+Y+HARRVSI
Sbjct: 301 RAFGKKSFNALRDRIEEIINGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVLP 420
           EIVGKENT+VSPN MGSEDFAFF DRVPGSF+ LGTYNERIGAIYPPHSP +KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFVFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSTS 442
           IGAAIHAAFAYSYLLNSSSTS
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Tan0005969 vs. NCBI nr
Match: XP_022985573.1 (IAA-amino acid hydrolase ILR1-like 1 [Cucurbita maxima])

HSP 1 Score: 790.4 bits (2040), Expect = 8.1e-225
Identity = 399/441 (90.48%), Postives = 416/441 (94.33%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLETIVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWMK 60
           MELLYF+II SIF       KLE  VHGTT SS+QT+Q+SSL H I ELANDPTTSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTTASSVQTSQNSSLRHQILELANDPTTSNWMK 60

Query: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEFETSRLIR ELDNLGVSYRWPVAGTG+VASVGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRLELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGVE ILGLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPD  TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDLVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVSI 360
           RAFSKKSFNALRDRIEE+I+GQAAVHRCT EIDFLGKEHPT+PPTINNEK+Y+HARRVSI
Sbjct: 301 RAFSKKSFNALRDRIEEIISGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVLP 420
           EIVGKENT+VSPN MGSEDFAFF DRVPGSF+ LGTYNERIGAIYPPHSP +KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFIFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSTS 442
           IGAAIHAAFAYSYLLNSSSTS
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Tan0005969 vs. NCBI nr
Match: XP_023527394.1 (IAA-amino acid hydrolase ILR1-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.9 bits (2036), Expect = 2.4e-224
Identity = 398/441 (90.25%), Postives = 414/441 (93.88%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLETIVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWMK 60
           MELLYF+II SIF       KLE  VHGT  SS+QT+Q+SSL H I ELANDP TSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTISSSVQTSQNSSLRHQILELANDPITSNWMK 60

Query: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTG+VASVGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGVE ILGLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLD QVVSVAMVQAGTALNVIPDS TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDPQVVSVAMVQAGTALNVIPDSVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVSI 360
           RAF KKSFNALRDRIEE+INGQAAVHRCT EIDFLGKEHPT+PPTINNEK+Y+HARRVSI
Sbjct: 301 RAFGKKSFNALRDRIEEIINGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVLP 420
           EIVGKENT+VSPN MGSEDFAFF DRVPGSF+ LGTYNERIGAIYPPHSP +KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFVFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSTS 442
           IGAAIHAAFAYSYLLNSSSTS
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Tan0005969 vs. NCBI nr
Match: XP_038893400.1 (IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida])

HSP 1 Score: 762.7 bits (1968), Expect = 1.8e-216
Identity = 387/441 (87.76%), Postives = 407/441 (92.29%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLETIVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWMK 60
           ME LY +II SIF+P +FSL  E  +H TTP S+QTN DSSL H I + AN PTT NWMK
Sbjct: 1   MEFLY-IIITSIFLPLTFSLNFEATLHVTTPISLQTNHDSSLRHQILDFANHPTTVNWMK 60

Query: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRAD 120
           TIRRKIHENPELA+EEFETSRLIR ELDNL VSYRWPVAGTG+VA VGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAFEEFETSRLIRHELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRAD 120

Query: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGA KIL+QLRQKLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGAAKILNQLRQKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERG GAKDMIKEGVLDGVEAI  LH++HKYPVGVVASR GEFLAGCGSFK KIKGKGGHA
Sbjct: 181 ERGRGAKDMIKEGVLDGVEAIFALHLVHKYPVGVVASRAGEFLAGCGSFKVKIKGKGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTF 300
           AIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTF
Sbjct: 241 AIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTF 300

Query: 301 RAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVSI 360
           RAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPT+PPTIN+EKIYEH RRVS+
Sbjct: 301 RAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTIPPTINDEKIYEHVRRVSM 360

Query: 361 EIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVLP 420
           EIVGKE  E SP+LMGSEDFAFF D+VPGSFL LGTYNERIGAI+PPHSP YKIDENVLP
Sbjct: 361 EIVGKEKIEESPSLMGSEDFAFFSDKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSTS 442
           IGAAIHAAFAYSYLLNSSSTS
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 440

BLAST of Tan0005969 vs. NCBI nr
Match: XP_004135471.3 (IAA-amino acid hydrolase ILR1-like 4 isoform X2 [Cucumis sativus] >KAE8648727.1 hypothetical protein Csa_009018 [Cucumis sativus])

HSP 1 Score: 755.7 bits (1950), Expect = 2.2e-214
Identity = 380/442 (85.97%), Postives = 408/442 (92.31%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLET-IVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWM 60
           ME LY +   SIF+P +FSL LE+  +HGTT  S+ TN  SSLTH I +LAN PT  NWM
Sbjct: 38  MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQIIDLANHPTAVNWM 97

Query: 61  KTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRA 120
           KTIRRKIHENPELA+EEFETSRLIRQELDNL VSYRWPVAGTG+VA VGSGSPPFVALRA
Sbjct: 98  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 157

Query: 121 DMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV+GKMHACSHDAHVAMLLGATKIL+QLR KLQGTVVL+FQPA
Sbjct: 158 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 217

Query: 181 EERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMI EG LDGVEAI GLH++H+YPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 218 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 277

Query: 241 AAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGT 300
           AAIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAGTALNVIP+SATIAGT
Sbjct: 278 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 337

Query: 301 FRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVS 360
           FRAFSKKSFNALRDRIEEVINGQA VHRCTAEIDFLGKEHPT+PP +N+EKIYEH RRVS
Sbjct: 338 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPMVNDEKIYEHVRRVS 397

Query: 361 IEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVL 420
           +EIVGKE T+VSP LMGSEDFAFF D+VPGSFL LGTYNERIGAI+PPHSPRYKIDENVL
Sbjct: 398 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 457

Query: 421 PIGAAIHAAFAYSYLLNSSSTS 442
           P+GAAIHAA AYSYLLNSSSTS
Sbjct: 458 PLGAAIHAAVAYSYLLNSSSTS 479

BLAST of Tan0005969 vs. ExPASy TrEMBL
Match: A0A6J1FSW3 (IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111446993 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.2e-226
Identity = 401/441 (90.93%), Postives = 417/441 (94.56%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLETIVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWMK 60
           MELLYF+II SIF       KLE  VHGTT SS+QT+Q+SSL H I ELANDPTTSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTTASSVQTSQNSSLRHQILELANDPTTSNWMK 60

Query: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTG+VASVGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGVE ILGLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDS TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVSI 360
           RAF KKSFNALRDRIEE+INGQAAVHRCT EIDFLGKEHPT+PPTINNEK+Y+HARRVSI
Sbjct: 301 RAFGKKSFNALRDRIEEIINGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVLP 420
           EIVGKENT+VSPN MGSEDFAFF DRVPGSF+ LGTYNERIGAIYPPHSP +KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFVFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSTS 442
           IGAAIHAAFAYSYLLNSSSTS
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Tan0005969 vs. ExPASy TrEMBL
Match: A0A6J1JE04 (IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111483601 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.9e-225
Identity = 399/441 (90.48%), Postives = 416/441 (94.33%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLETIVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWMK 60
           MELLYF+II SIF       KLE  VHGTT SS+QT+Q+SSL H I ELANDPTTSNWMK
Sbjct: 1   MELLYFLIIISIF-------KLEAPVHGTTASSVQTSQNSSLRHQILELANDPTTSNWMK 60

Query: 61  TIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRAD 120
           TIRRKIHENPELAYEEFETSRLIR ELDNLGVSYRWPVAGTG+VASVGSGSPPFVALRAD
Sbjct: 61  TIRRKIHENPELAYEEFETSRLIRLELDNLGVSYRWPVAGTGIVASVGSGSPPFVALRAD 120

Query: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAE 180
           MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR+KLQGTVVLIFQPAE
Sbjct: 121 MDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLREKLQGTVVLIFQPAE 180

Query: 181 ERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHA 240
           ERGGGAKDMIKEGVLDGVE ILGLH+MH+YPVGVVASRPGEFLAGCGSFKAKIKG+GGHA
Sbjct: 181 ERGGGAKDMIKEGVLDGVETILGLHVMHRYPVGVVASRPGEFLAGCGSFKAKIKGRGGHA 240

Query: 241 AIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTF 300
           AIP HSIDPVLAAS AIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPD  TIAGTF
Sbjct: 241 AIPHHSIDPVLAASTAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDLVTIAGTF 300

Query: 301 RAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVSI 360
           RAFSKKSFNALRDRIEE+I+GQAAVHRCT EIDFLGKEHPT+PPTINNEK+Y+HARRVSI
Sbjct: 301 RAFSKKSFNALRDRIEEIISGQAAVHRCTVEIDFLGKEHPTIPPTINNEKMYDHARRVSI 360

Query: 361 EIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVLP 420
           EIVGKENT+VSPN MGSEDFAFF DRVPGSF+ LGTYNERIGAIYPPHSP +KIDENVLP
Sbjct: 361 EIVGKENTKVSPNFMGSEDFAFFSDRVPGSFIFLGTYNERIGAIYPPHSPHFKIDENVLP 420

Query: 421 IGAAIHAAFAYSYLLNSSSTS 442
           IGAAIHAAFAYSYLLNSSSTS
Sbjct: 421 IGAAIHAAFAYSYLLNSSSTS 434

BLAST of Tan0005969 vs. ExPASy TrEMBL
Match: A0A0A0KQC3 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G603370 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 1.1e-214
Identity = 380/442 (85.97%), Postives = 408/442 (92.31%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLET-IVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWM 60
           ME LY +   SIF+P +FSL LE+  +HGTT  S+ TN  SSLTH I +LAN PT  NWM
Sbjct: 35  MEFLYILFFISIFLPLTFSLNLESPDLHGTTSISLPTNHTSSLTHQIIDLANHPTAVNWM 94

Query: 61  KTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRA 120
           KTIRRKIHENPELA+EEFETSRLIRQELDNL VSYRWPVAGTG+VA VGSGSPPFVALRA
Sbjct: 95  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 154

Query: 121 DMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV+GKMHACSHDAHVAMLLGATKIL+QLR KLQGTVVL+FQPA
Sbjct: 155 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 214

Query: 181 EERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMI EG LDGVEAI GLH++H+YPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 215 EEKGGGAKDMINEGALDGVEAIFGLHVVHEYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 274

Query: 241 AAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGT 300
           AAIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAGTALNVIP+SATIAGT
Sbjct: 275 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 334

Query: 301 FRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVS 360
           FRAFSKKSFNALRDRIEEVINGQA VHRCTAEIDFLGKEHPT+PP +N+EKIYEH RRVS
Sbjct: 335 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEHPTIPPMVNDEKIYEHVRRVS 394

Query: 361 IEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVL 420
           +EIVGKE T+VSP LMGSEDFAFF D+VPGSFL LGTYNERIGAI+PPHSPRYKIDENVL
Sbjct: 395 MEIVGKEKTKVSPRLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPRYKIDENVL 454

Query: 421 PIGAAIHAAFAYSYLLNSSSTS 442
           P+GAAIHAA AYSYLLNSSSTS
Sbjct: 455 PLGAAIHAAVAYSYLLNSSSTS 476

BLAST of Tan0005969 vs. ExPASy TrEMBL
Match: A0A5A7ST02 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003470 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.5e-213
Identity = 380/442 (85.97%), Postives = 407/442 (92.08%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLET-IVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWM 60
           ME LY +   SIF+P SFSL LE+  ++ TT  S+ TN  SSL H I +LAN PTT NWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRA 120
           KTIRRKIHENPELA+EEFETSRLIRQELDNL VSYRWPVAGTG+VA VGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV+GKMHACSHDAHVAMLLGATKIL+QLR KLQGTVVL+FQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMI EG LDGVEAI GLH++HKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGT 300
           AAIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAGTALNVIP+SATIAGT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVS 360
           FRAFSKKSFNALRDRIEEVINGQA VHRCTAEIDFLGKE+PT+PP IN+EKIYEH RRVS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 IEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVL 420
           +EIVGKE T+VSP+LMGSEDFAFF D+VPGSFL LGTYNERIGAI+PPHSP YKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAAFAYSYLLNSSSTS 442
           PIGAAIHAA AYSYLLNSSSTS
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTS 472

BLAST of Tan0005969 vs. ExPASy TrEMBL
Match: A0A5D3CVM4 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00690 PE=3 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 3.8e-212
Identity = 379/442 (85.75%), Postives = 406/442 (91.86%), Query Frame = 0

Query: 1   MELLYFVIIASIFVPFSFSLKLET-IVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWM 60
           ME LY +   SIF+P SFSL LE+  ++ TT  S+ TN  SSL H I +LAN PTT NWM
Sbjct: 31  MEFLYILFFISIFLPLSFSLNLESPDLNETTSISLHTNHTSSLRHQILDLANHPTTVNWM 90

Query: 61  KTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRA 120
           KTIRRKIHENPELA+EEFETSRLIRQELDNL VSYRWPVAGTG+VA VGSGSPPFVALRA
Sbjct: 91  KTIRRKIHENPELAFEEFETSRLIRQELDNLRVSYRWPVAGTGVVAFVGSGSPPFVALRA 150

Query: 121 DMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPA 180
           DMDALPIEELVEWEHKSKV+GKMHACSHDAHVAMLLGATKIL+QLR KLQGTVVL+FQPA
Sbjct: 151 DMDALPIEELVEWEHKSKVEGKMHACSHDAHVAMLLGATKILNQLRHKLQGTVVLVFQPA 210

Query: 181 EERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGH 240
           EE+GGGAKDMI EG LDGVEAI GLH++HKYPVGVVASR GEFLAGCGSFKAKIKGKGGH
Sbjct: 211 EEKGGGAKDMINEGALDGVEAIFGLHVVHKYPVGVVASRTGEFLAGCGSFKAKIKGKGGH 270

Query: 241 AAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGT 300
           AAIPQ SIDP+LAASAAI+SLQ+IVSREIDPLDSQVVSVAMVQAGTALNVIP+SATIAGT
Sbjct: 271 AAIPQDSIDPILAASAAIISLQSIVSREIDPLDSQVVSVAMVQAGTALNVIPESATIAGT 330

Query: 301 FRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVS 360
           FRAFSKKSFNALRDRIEEVINGQA VHRCTAEIDFLGKE+PT+PP IN+EKIYEH RRVS
Sbjct: 331 FRAFSKKSFNALRDRIEEVINGQAVVHRCTAEIDFLGKEYPTIPPMINDEKIYEHVRRVS 390

Query: 361 IEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVL 420
           +EIVGKE T+VSP+LMGSEDFAFF D+VPGSFL LGTYNERIGAI+PPHSP YKIDENVL
Sbjct: 391 MEIVGKEKTKVSPSLMGSEDFAFFADKVPGSFLFLGTYNERIGAIHPPHSPHYKIDENVL 450

Query: 421 PIGAAIHAAFAYSYLLNSSSTS 442
           PIGAAIHAA AYSYLLNSSSTS
Sbjct: 451 PIGAAIHAAVAYSYLLNSSSTS 472

BLAST of Tan0005969 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 456.4 bits (1173), Expect = 2.6e-128
Identity = 235/431 (54.52%), Postives = 306/431 (71.00%), Query Frame = 0

Query: 11  SIFVPFSFSLKLETIVHGTTPSSIQTNQDSSLTHLIQELANDPTTSNWMKTIRRKIHENP 70
           S F   SF L L  +    T  S  +N  S +      LA      +WM  IRR+IHENP
Sbjct: 2   SFFKWVSFVLILHLL--NPTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIHENP 61

Query: 71  ELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASVGSGSPPFVALRADMDALPIEELV 130
           EL YEE ETS+L+R EL+ +GVSY++PVA TG+V  VG+G  PFVALRADMDAL ++E+V
Sbjct: 62  ELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMV 121

Query: 131 EWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMI 190
           EWEHKSKV GKMHAC HDAH  MLLGA K+L +  ++LQGTVVL+FQPAEE GGGAK ++
Sbjct: 122 EWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIV 181

Query: 191 KEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPV 250
           + GVL+ V AI GLH+ ++  +G V+SR G  LAG G FKAKI GKGGHAA+PQH+IDP+
Sbjct: 182 EAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPI 241

Query: 251 LAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTALNVIPDSATIAGTFRAFSKKSFNA 310
           LAAS  IVSLQ++VSRE DPLDSQVV+VA  + G A NVIPDS TI GTFRAFS KSF  
Sbjct: 242 LAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQ 301

Query: 311 LRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTINNEKIYEHARRVSIEIVGKENTEV 370
           L+ RIE+VI  QA+V+ C A +DF+ +E P  PPT+N++ +++  + VS +++G EN   
Sbjct: 302 LKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVE 361

Query: 371 SPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPPHSPRYKIDENVLPIGAAIHAAFA 430
              LMGSEDF+F+   +PG F  +G  N+    +  PHSP ++++E +LP GA++HA+ A
Sbjct: 362 MQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMA 421

Query: 431 YSYLLNSSSTS 442
             YLL   +++
Sbjct: 422 TRYLLELKAST 430

BLAST of Tan0005969 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 451.8 bits (1161), Expect = 6.4e-127
Identity = 229/400 (57.25%), Postives = 287/400 (71.75%), Query Frame = 0

Query: 40  SSLTHLIQELANDPTTSNWMKTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVA 99
           S +   + E A  P   +WM  IRRKIHENPEL YEE ETS+LIR EL+ +G+ YR+PVA
Sbjct: 32  SQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSKLIRSELELIGIKYRYPVA 91

Query: 100 GTGLVASVGSGSPPFVALRADMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATK 159
            TG++  +G+G PPFVALRADMDALPI+E VEWEHKSK+ GKMHAC HD HV MLLGA K
Sbjct: 92  ITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEHKSKIAGKMHACGHDGHVTMLLGAAK 151

Query: 160 ILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRP 219
           ILH+ R  LQGTVVLIFQPAEE   GAK M +EG L  VEAI G+H+  + P G  ASR 
Sbjct: 152 ILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAIFGIHLSARIPFGKAASRA 211

Query: 220 GEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVA 279
           G FLAG G F+A I GKGGHAAIPQH+IDPV+AAS+ ++SLQ +VSRE DPLDS+VV+V+
Sbjct: 212 GSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQQLVSRETDPLDSKVVTVS 271

Query: 280 MVQAGTALNVIPDSATIAGTFRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEH 339
            V  G A NVIPDS TI GT RAF+   F  L+ R++EVI  QAAVHRC A ++      
Sbjct: 272 KVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQRVKEVITKQAAVHRCNASVNLTPNGR 331

Query: 340 PTVPPTINNEKIYEHARRVSIEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNE 399
             +PPT+NN+ +Y+  ++V  +++G+E    +  +MGSEDF++F + +PG F LLG  +E
Sbjct: 332 EPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQDE 391

Query: 400 RIGAIYPPHSPRYKIDENVLPIGAAIHAAFAYSYLLNSSS 440
             G     HSP Y+I+E+VLP GAAIHA+ A  YL   +S
Sbjct: 392 TNG-YASSHSPLYRINEDVLPYGAAIHASMAVQYLKEKAS 428

BLAST of Tan0005969 vs. TAIR 10
Match: AT5G56650.1 (IAA-leucine resistant (ILR)-like 1 )

HSP 1 Score: 444.1 bits (1141), Expect = 1.3e-124
Identity = 229/392 (58.42%), Postives = 283/392 (72.19%), Query Frame = 0

Query: 48  ELANDPTTSNWMKTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVASV 107
           ELA  P   + M  IRRKIHENPEL YEEFETS+ IR ELD +GV YR+PVA TG++  +
Sbjct: 39  ELAKSPEVFDSMVRIRRKIHENPELGYEEFETSKFIRSELDLIGVKYRFPVAITGIIGYI 98

Query: 108 GSGSPPFVALRADMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLRQK 167
           G+G PPFVALRADMDALPI+E VEWEHKSK  GKMHAC HD HVAMLLGA KIL Q RQ 
Sbjct: 99  GTGEPPFVALRADMDALPIQEAVEWEHKSKNPGKMHACGHDGHVAMLLGAAKILQQHRQH 158

Query: 168 LQGTVVLIFQPAEERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAGCG 227
           LQGTVVLIFQPAEE   GAK M +EG L  VEAI G+H+  + P G  AS  G F+AG G
Sbjct: 159 LQGTVVLIFQPAEEGLSGAKMMREEGALKNVEAIFGIHLSPRTPFGKAASLAGSFMAGAG 218

Query: 228 SFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGTAL 287
           +F+A I GKGGHAAIPQH+IDPV+AAS+ ++SLQ++VSRE DP DS+VV+V  V  G A 
Sbjct: 219 AFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQHLVSRETDPSDSKVVTVTKVNGGNAF 278

Query: 288 NVIPDSATIAGTFRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPTIN 347
           NVIPDS TI GT RAF+   F  L++RI+E+I  QAAVHRC A ++     +  +PPT+N
Sbjct: 279 NVIPDSITIGGTLRAFT--GFTQLQERIKEIITKQAAVHRCNASVNLAPNGNQPMPPTVN 338

Query: 348 NEKIYEHARRVSIEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIYPP 407
           N  +Y+  ++V  +++G+E    +   MGSEDF++F + +PG F LLG  +E  G     
Sbjct: 339 NMDLYKKFKKVVRDLLGQEAFVEAVPEMGSEDFSYFAETIPGHFSLLGMQDETQG-YASS 398

Query: 408 HSPRYKIDENVLPIGAAIHAAFAYSYLLNSSS 440
           HSP Y+I+E+VLP GAAIHA  A  YL + +S
Sbjct: 399 HSPHYRINEDVLPYGAAIHATMAVQYLKDKAS 427

BLAST of Tan0005969 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 221/408 (54.17%), Postives = 291/408 (71.32%), Query Frame = 0

Query: 33  SIQTNQDSSLTHLIQELANDPTTSNWMKTIRRKIHENPELAYEEFETSRLIRQELDNLGV 92
           S  +N  S +      LA      +WM  IRR+IHENPEL YEE ETS+L++ ELD +GV
Sbjct: 22  SCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGV 81

Query: 93  SYRWPVAGTGLVASVGSGSPPFVALRADMDALPIEELVEWEHKSKVKGKMHACSHDAHVA 152
           SY+ PVA TG++  VG+G  PFVALRADMDALPI+E+VEWEHKSK+ GKMHAC HDAH  
Sbjct: 82  SYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHTT 141

Query: 153 MLLGATKILHQLRQKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVEAILGLHIMHKYPV 212
           MLLGA K+L + +++LQGTV+L+FQPAEE G GAK +++ GVL+ V AI GLH+ +   +
Sbjct: 142 MLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLGL 201

Query: 213 GVVASRPGEFLAGCGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLD 272
           G ++SR G  +AG G FKA I GKGGHAA+PQ +IDPVLAAS  I+SLQ++VSRE DPLD
Sbjct: 202 GQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPLD 261

Query: 273 SQVVSVAMVQAGTALNVIPDSATIAGTFRAFSKKSFNALRDRIEEVINGQAAVHRCTAEI 332
           SQVV+VA  +   A NVIPDS TI GTFRA   KSF  L+ RI +VI  QA+V+ C A +
Sbjct: 262 SQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNATV 321

Query: 333 DFLGKEHPTVPPTINNEKIYEHARRVSIEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFL 392
           DFL  E P  PPT+NN+ ++   + VS++++G EN   +  +M SEDFAF+   +PG F 
Sbjct: 322 DFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFS 381

Query: 393 LLGTYNERIGAIYPPHSPRYKIDENVLPIGAAIHAAFAYSYLLNSSST 441
            +G  N+    +  PHSP ++++E +LP GA++ A+ A  YLL+SSS+
Sbjct: 382 FVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSSSS 429

BLAST of Tan0005969 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 427.9 bits (1099), Expect = 9.9e-120
Identity = 210/394 (53.30%), Postives = 275/394 (69.80%), Query Frame = 0

Query: 46  IQELANDPTTSNWMKTIRRKIHENPELAYEEFETSRLIRQELDNLGVSYRWPVAGTGLVA 105
           I  L   P    W+K +RR IHENPELA+EE+ETSRLIR ELD +G+ YR+P+A TG+ A
Sbjct: 76  ILRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIRA 135

Query: 106 SVGSGSPPFVALRADMDALPIEELVEWEHKSKVKGKMHACSHDAHVAMLLGATKILHQLR 165
            +GSG PPFVA+RADMDALPI+E VEWEH SKV GKMHAC HDAHV MLLGA  IL    
Sbjct: 136 WIGSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHILKARE 195

Query: 166 QKLQGTVVLIFQPAEERGGGAKDMIKEGVLDGVEAILGLHIMHKYPVGVVASRPGEFLAG 225
             L+GTVVL+FQPAEE G GAK+MI++G LD VEAI  +H+ H +P GV+ SR G  LAG
Sbjct: 196 HLLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLAG 255

Query: 226 CGSFKAKIKGKGGHAAIPQHSIDPVLAASAAIVSLQNIVSREIDPLDSQVVSVAMVQAGT 285
           CG F+A I  +    A      + +LAAS+A++SLQ IVSRE  PLDSQVVSV     G 
Sbjct: 256 CGIFRAVITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSVTSFDGGH 315

Query: 286 ALNVIPDSATIAGTFRAFSKKSFNALRDRIEEVINGQAAVHRCTAEIDFLGKEHPTVPPT 345
           +L+V PD+  + GTFRAFS  SF  L+ RI+EV+  Q  V  C A ++F  K++   PPT
Sbjct: 316 SLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPPT 375

Query: 346 INNEKIYEHARRVSIEIVGKENTEVSPNLMGSEDFAFFLDRVPGSFLLLGTYNERIGAIY 405
            NN+  Y H ++V+I+++G  +  ++P +MG+EDFAF+ + +P +F  +G  NE +G+++
Sbjct: 376 TNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSVH 435

Query: 406 PPHSPRYKIDENVLPIGAAIHAAFAYSYLLNSSS 440
             HSP + IDE+ LP+GAA+HAA A  YL +  S
Sbjct: 436 IAHSPHFMIDEDSLPVGAAVHAAVAERYLNDKHS 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84XG97.1e-13160.21IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=I... [more]
Q8S9S47.1e-13160.21IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
O043733.6e-12754.52IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=... [more]
P549709.0e-12657.25IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=... [more]
P549691.9e-12358.42IAA-amino acid hydrolase ILR1-like 1 OS=Arabidopsis thaliana OX=3702 GN=ILL1 PE=... [more]
Match NameE-valueIdentityDescription
XP_022941713.12.5e-22690.93IAA-amino acid hydrolase ILR1-like 1 [Cucurbita moschata] >KAG6600138.1 IAA-amin... [more]
XP_022985573.18.1e-22590.48IAA-amino acid hydrolase ILR1-like 1 [Cucurbita maxima][more]
XP_023527394.12.4e-22490.25IAA-amino acid hydrolase ILR1-like 1 [Cucurbita pepo subsp. pepo][more]
XP_038893400.11.8e-21687.76IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida][more]
XP_004135471.32.2e-21485.97IAA-amino acid hydrolase ILR1-like 4 isoform X2 [Cucumis sativus] >KAE8648727.1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FSW31.2e-22690.93IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita moschata OX=3662 GN=LOC1114469... [more]
A0A6J1JE043.9e-22590.48IAA-amino acid hydrolase ILR1-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111483601... [more]
A0A0A0KQC31.1e-21485.97M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G603370 P... [more]
A0A5A7ST024.5e-21385.97IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3CVM43.8e-21285.75IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT1G51760.12.6e-12854.52peptidase M20/M25/M40 family protein [more]
AT5G56660.16.4e-12757.25IAA-leucine resistant (ILR)-like 2 [more]
AT5G56650.11.3e-12458.42IAA-leucine resistant (ILR)-like 1 [more]
AT1G51780.11.2e-12254.17IAA-leucine resistant (ILR)-like gene 5 [more]
AT1G44350.19.9e-12053.30IAA-leucine resistant (ILR)-like gene 6 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 225..323
e-value: 8.0E-11
score: 41.8
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 45..438
e-value: 2.3E-129
score: 429.6
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 60..422
e-value: 9.2E-120
score: 398.1
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 6..442
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 61..419
e-value: 3.5E-140
score: 468.9
NoneNo IPR availableGENE3D3.30.70.360coord: 224..342
e-value: 3.5E-140
score: 468.9
NoneNo IPR availablePANTHERPTHR11014:SF147IAA-AMINO ACID HYDROLASE ILR1-LIKE 2coord: 6..442
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 56..436
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 116..430
e-value: 1.1E-33
score: 117.2
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 59..434
e-value: 0.0
score: 655.544
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 224..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0005969.1Tan0005969.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity