Tan0005847 (gene) Snake gourd v1

BLAST of Tan0005847 vs. ExPASy Swiss-Prot
Match: Q9LV40 (Rho guanine nucleotide exchange factor 8 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF8 PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 5.1e-146
Identity = 296/534 (55.43%), Postives = 377/534 (70.60%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPG---RQAHSMILESGQEGHASRDSLEPQR----I 60
           MV AL  +  +  S+SFN KRM +      +Q+ ++++E+G       ++ E Q     +
Sbjct: 1   MVAAL--ERGLSASKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESNTPESQNSDSFV 60

Query: 61  GWALSDNNGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALA 120
              +  +   +S L   G R+     +  +D+E+MK +FAKLLLGEDMSGGGKGVSSALA
Sbjct: 61  ESPVESSLPMISPLTRPGKRSE----RQQADMEMMKDRFAKLLLGEDMSGGGKGVSSALA 120

Query: 121 LSNAITNLAASTFGEQ-KLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEM 180
           LSNAITNLAAS FGEQ KL+ +   ++ RW++EI+W LSV DHIVEFVPS Q SKDG+  
Sbjct: 121 LSNAITNLAASIFGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSKDGVCT 180

Query: 181 EIMVTLQRKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKD-NTQRNGDKW 240
           EIMVT QR DLLMNIPALRKLDAML+  LD F   +EFWYVSRD+E  +     R  DKW
Sbjct: 181 EIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDRTNDKW 240

Query: 241 WIPAVKVPPNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGK 300
           W+P VKVPP GLSE +R+ L FQKDSV Q+ KAAMAINAQVL+EME+P+SYI+SLPKNG+
Sbjct: 241 WLPPVKVPPGGLSEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSLPKNGR 300

Query: 301 TSLGDSIYKSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSW 360
            SLGDSIYKSIT+++F+ ++FL  +D+STEHKV+DLKNRIEAS+VIWKRK+H KD KSSW
Sbjct: 301 ASLGDSIYKSITEEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKDTKSSW 360

Query: 361 GSAVSFEKREIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVES 420
           GSAVS EKRE+FEER E+IL L+KQ FPG+ QS+LD+SKIQ NKDVGQA+LESYSR++ES
Sbjct: 361 GSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYSRILES 420

Query: 421 LAFNIMSRIEDVLYAHTIAREASI--------EQSFENISWMRMLSSNGSEA------LK 480
           LA+ +MSRIEDVLY  T+A + ++         ++ E  S     S++G EA       K
Sbjct: 421 LAYTVMSRIEDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSK 480

Query: 481 TLSDFMGWDVDKDDLSINESCCKEEEDINLMSKPNDI--KLESLSALRSPTARH 509
           TL DFMGW+ +      ++   K  +  NL  K      KLE+L+  RSP  RH
Sbjct: 481 TLLDFMGWNDNS-----SKGGDKPTKSPNLTPKKLSYLEKLENLNGFRSPKDRH 523

BLAST of Tan0005847 vs. ExPASy Swiss-Prot
Match: Q9SVQ3 (Rop guanine nucleotide exchange factor 9 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF9 PE=1 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.2e-141
Identity = 295/529 (55.77%), Postives = 371/529 (70.13%), Query Frame = 0

Query: 11  IQQSRSFNLKRMVE-LPGR-QAHSMILESGQEGHASRDSLEPQRIGWALSDNNGPVSCLD 70
           I  + SFNL RM +  PG+ Q    + E+      ++DSL     G +  + + P++   
Sbjct: 9   ISITSSFNLDRMFDSSPGKEQQQPHLAETTMPESQTQDSL-----GGSPVETSRPMT--- 68

Query: 71  AHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITNLAASTFGE 130
           +  I  R  + Q  S+ E+MK +F KLLLGEDMSGGGKGVSSALALSNAITNLAAS FGE
Sbjct: 69  SRLISRRQDKQQ--SETEMMKDRFTKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGE 128

Query: 131 Q-KLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQRKDLLMNI 190
           Q KL+ ++P ++ RW++EI+W LSV DHIVEFVPS Q SK+G+  EIMVT QR DLLMNI
Sbjct: 129 QTKLQPMAPDRRARWKKEIDWLLSVTDHIVEFVPSQQISKEGVCTEIMVTRQRGDLLMNI 188

Query: 191 PALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKD-NTQRNGDKWWIPAVKVPPNGLSEV 250
           PALRKLDAML+  LD F   +EFWYVSRD+E  K    +R  DKWW+P VKVPPNGLSE 
Sbjct: 189 PALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGKQARNERTKDKWWLPPVKVPPNGLSES 248

Query: 251 TRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYKSITDDY 310
            R+ L FQKDSV+Q+ KAAMAINAQVL+EM +PDSYIESLPKNG+ SLGDS+YKSIT+++
Sbjct: 249 ARRMLHFQKDSVSQVQKAAMAINAQVLSEMAIPDSYIESLPKNGRVSLGDSLYKSITEEW 308

Query: 311 FNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKREIFEER 370
           F+ ++FL ++DLSTEHKV+D+KNRIEASIVIWKRK+H KD KSSWGSAVS EKRE+FEER
Sbjct: 309 FDPEQFLSTLDLSTEHKVLDVKNRIEASIVIWKRKLHLKDNKSSWGSAVSLEKRELFEER 368

Query: 371 VESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRIEDVLYA 430
            E+IL L+KQ FPG+ QS+LD+SKIQ+NKDVG A+LESYSR++ESL +  MSRI+DVLYA
Sbjct: 369 AETILVLLKQKFPGLPQSSLDISKIQYNKDVGHAVLESYSRILESLGYTEMSRIDDVLYA 428

Query: 431 HTIARE----------------------ASIEQSFENISWMRMLSSNGSEALKTLSDFMG 490
            ++AR+                       S   S E I   ++ S N S+   TL DF+G
Sbjct: 429 DSLARKQCTGEETSDGGKIATETDSASAGSSNYSGEEIE--KLESQNSSKT--TLLDFIG 488

Query: 491 WDVDKDDLSINESCCKEEEDINLMSKPNDI----KLESLSALRSPTARH 509
           W    D+ S  +S   E+   +    P  +    KLE+L+  RSP  RH
Sbjct: 489 W---SDNSSKGQS---EKPPKSPRMTPKKLSYLEKLENLNGFRSPKDRH 517

BLAST of Tan0005847 vs. ExPASy Swiss-Prot
Match: Q9CA89 (Rop guanine nucleotide exchange factor 12 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF12 PE=1 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.5e-134
Identity = 281/530 (53.02%), Postives = 361/530 (68.11%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLK---RMVELPGRQAHSMILESG-QEGHASRDSLEPQRI--- 60
           MVRA   +    +SR FN K          R   S+ +E+G  E        EP  I   
Sbjct: 1   MVRASEQEQETYRSRLFNFKWRNNDNNSATRHNKSLSVETGLDEAATGSHDAEPLTIIHP 60

Query: 61  --GWALSDNNGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSA 120
             G  LS +    + L A        R Q  +D+E MK +F+KLLLGED SGGGKGVSSA
Sbjct: 61  SQGPPLSRSAADEAVLAALAASQARER-QLLADMEQMKERFSKLLLGEDNSGGGKGVSSA 120

Query: 121 LALSNAITNLAASTFGEQ-KLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGI 180
           LALSNAITNLAAS FGEQ +LE +   ++ RWR+EI+W LSV D++VEF PS Q +KDG 
Sbjct: 121 LALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLSVTDYVVEFAPSQQKNKDGT 180

Query: 181 EMEIMVTLQRKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNTQRNGDK 240
            MEIM T QR DL MNIPAL+KLDAML+  L+ F  Q EF Y+S+D+     + +RN +K
Sbjct: 181 NMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFSYISKDSPDL--DGKRNDEK 240

Query: 241 WWIPAVKVPPNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNG 300
           WWIP VKVPP+GLSE +R++L +QKD VNQ+LKAAMAINAQVL EME+P+SYI+SLPKNG
Sbjct: 241 WWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINAQVLFEMEIPESYIDSLPKNG 300

Query: 301 KTSLGDSIYKSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSS 360
           + SLGD +YK+IT D+F+ D+FL SMD+S+EHK+VDLKNRIEASI+IWKRKM +KD KSS
Sbjct: 301 RASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNRIEASIIIWKRKMVYKDNKSS 360

Query: 361 --WGSAVSFEKREIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRV 420
             W S VS EKRE+FEER E+IL ++KQ +PGISQS+LD+SKIQ N+DVGQA+LESYSR+
Sbjct: 361 APWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDISKIQFNEDVGQAVLESYSRI 420

Query: 421 VESLAFNIMSRIEDVLYAHTIAREASIEQSFENISWMRMLSSNGSEALKTLSDFMGWDVD 480
           +ESLA+ ++SRI+DVL A     + +     E  + +  +         TLSDFMGWD D
Sbjct: 421 LESLAYTVLSRIDDVLEADRAGNKRNTPLEAEEETLVGSM---------TLSDFMGWDFD 480

Query: 481 ---------KDDLSINESCCKEEEDINLMSKPNDIKLESLSALRSPTARH 509
                    K DL  ++   KE+  +++++      LE+L  ++SPTARH
Sbjct: 481 QAANAELESKKDLP-DDPLIKEK--LSVVTTKKTSYLETLGGVKSPTARH 515

BLAST of Tan0005847 vs. ExPASy Swiss-Prot
Match: Q9M811 (Rop guanine nucleotide exchange factor 11 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF11 PE=1 SV=2)

HSP 1 Score: 479.6 bits (1233), Expect = 4.5e-134
Identity = 270/442 (61.09%), Postives = 330/442 (74.66%), Query Frame = 0

Query: 85  LELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITNLAASTFGEQ-KLETLSPGKKTRWR 144
           +E MK KF+KLLLGEDMSGGGKGVSSALALSNAITNLAAS FGEQ +LE ++  +KTRWR
Sbjct: 105 MEQMKEKFSKLLLGEDMSGGGKGVSSALALSNAITNLAASAFGEQRRLEPMAVDRKTRWR 164

Query: 145 REIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQRKDLLMNIPALRKLDAMLLGYLDQ 204
           REI W +SVAD+IVEF P+ Q +KDG  ME+M T QR DLL NIPAL+KLDAMLL  LD+
Sbjct: 165 REIGWLISVADYIVEFAPTQQTNKDGTSMEVMSTRQRTDLLCNIPALKKLDAMLLDCLDK 224

Query: 205 FGKQHEFWYVSRDAESAKDNTQRNGDKWWIPAVKVPPNGLSEVTRKWLLFQKDSVNQILK 264
           F  Q EF+YV +D+  + +   RN +KWW+PAVKVPPNGLSE++R++L  QK+ VNQ+LK
Sbjct: 225 FKDQDEFYYVKKDSPDSCET--RNDEKWWLPAVKVPPNGLSEISRRFLQSQKECVNQVLK 284

Query: 265 AAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYKSITDDYFNTDEFLKSMDLSTEHK 324
           AAMAINAQVL+EME+P+SY+ESLPKNG+ SLGD IY+ IT + F+ D+FL  MDLS+EHK
Sbjct: 285 AAMAINAQVLSEMEIPESYLESLPKNGRASLGDVIYRMITVEMFDADQFLIEMDLSSEHK 344

Query: 325 VVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKREIFEERVESILFLIKQYFPGISQ 384
           ++DLKNRIEASIVIWKRKM  KD KS WGS VS EKRE FEER E+IL L+KQ FPGISQ
Sbjct: 345 ILDLKNRIEASIVIWKRKMVQKDTKSPWGSTVSIEKREQFEERAETILLLLKQGFPGISQ 404

Query: 385 SALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRIEDVLYAHTIAREASIEQSFENIS 444
           S+LD+SKIQ N+DVG AILESYSRV+ESLA  +MSRIEDVLYA  + +E +     +N  
Sbjct: 405 SSLDISKIQFNRDVGLAILESYSRVLESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRY 464

Query: 445 WMR--------MLSSNGSEALKTLSDFMGWDVDKDDLSINESCCKEEEDINLMSKPNDIK 504
            ++         LS     A  TLSD M W  +K++    ES   + E   L+SK   I 
Sbjct: 465 SLKENEKLREERLSFTEDMASGTLSDVMQWG-NKNNEMKKESFFGDREK-PLLSKVTGIM 524

Query: 505 --------LESLSALRSPTARH 509
                   L++L A+RSPTAR+
Sbjct: 525 TNNKKSSYLDNLGAMRSPTARY 542

BLAST of Tan0005847 vs. ExPASy Swiss-Prot
Match: Q1KS66 (Rop guanine nucleotide exchange factor 10 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF10 PE=2 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.2e-125
Identity = 257/454 (56.61%), Postives = 315/454 (69.38%), Query Frame = 0

Query: 83  SDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITNLAASTFGEQ-KLETLSPGKKTR 142
           SD+E+MK +FAKLLLGEDMSGGG G +SALALSNAIT LA S FGEQ KL+ + P  K  
Sbjct: 43  SDMEVMKERFAKLLLGEDMSGGGTGETSALALSNAITKLADSMFGEQMKLQPMYPETKEN 102

Query: 143 WRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQRKDLLMNIPALRKLDAMLLGYL 202
           WR+E+ W LSV DHIV+FVPS Q  K+G   EIMVT QR DLL NIPALRKLD++LL  L
Sbjct: 103 WRKEMGWLLSVIDHIVQFVPSRQMGKNGQFTEIMVTKQRDDLLTNIPALRKLDSVLLETL 162

Query: 203 DQFGKQHEFWYVSRDAESAKDNTQ-RNGDKWWIPAVKVPPNGLSEVTRKWLLFQKDSVNQ 262
           D F  Q +FWYV RD E A  N   R  + WW+P VKVP +GLSE +R+WL  QKDSV Q
Sbjct: 163 DNFKDQKDFWYVPRDMEDADHNGDWRRDENWWLPVVKVPTDGLSEESRRWLQNQKDSVAQ 222

Query: 263 ILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYKSITDDYFNTDEFLKSMDLST 322
           +LKAA AINA VL+EM VP++YI+SLPKNGKTSLGD +YKSIT++ F+ D F+  +DLST
Sbjct: 223 VLKAATAINAHVLSEMHVPENYIDSLPKNGKTSLGDFLYKSITEESFDPDYFVSFLDLST 282

Query: 323 EHKVVDLKNRIEASIVIWKRKM--HHKDGKSSWGSAVSFEKREIFEERVESILFLIKQYF 382
           EHKV+DLKNRIEAS+VIWKRKM    KDGKS WGS VS EKRE+FE R E+IL ++KQ F
Sbjct: 283 EHKVLDLKNRIEASMVIWKRKMCQKEKDGKSQWGSTVSLEKRELFEVRAETILVMLKQQF 342

Query: 383 PGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRIEDVLYAHTIAR------- 442
           PGI QS+L+VSKI++NKDVGQAILESYSRV+ESLA  IMSRIEDVL A  + +       
Sbjct: 343 PGIPQSSLEVSKIKNNKDVGQAILESYSRVLESLASKIMSRIEDVLEADRLVQRQLMGEA 402

Query: 443 ----EASIEQSFENISWMRMLSSNGSEALKTLSDFMGWDVDKDDLSINESC-------CK 502
               E+  E  +E    +    +  S   + LSDF+GW +  D    +           +
Sbjct: 403 ETRSESEAESEYEETEKVVAAETPNS---RKLSDFIGWRLSSDTKKHSSMSDIEFFHKVE 462

Query: 503 EEEDINLMSKPNDIK-----LESLSALRSPTARH 509
           +E++  +M  P  +      L  L  +RSP+ RH
Sbjct: 463 QEKEKPMMKSPRALPKKFSYLAKLENMRSPSDRH 493

BLAST of Tan0005847 vs. NCBI nr
Match: XP_038890636.1 (rho guanine nucleotide exchange factor 8-like [Benincasa hispida])

HSP 1 Score: 871.7 bits (2251), Expect = 3.1e-249
Identity = 452/526 (85.93%), Postives = 479/526 (91.06%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSDN 60
           MVRALI QP++QQSRSF LKRMVELPGRQ HS+ILESGQEG+ SRDS EPQRIGWALSDN
Sbjct: 1   MVRALIQQPSVQQSRSFQLKRMVELPGRQTHSLILESGQEGNGSRDSSEPQRIGWALSDN 60

Query: 61  NGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN 120
             PV CLD HGIRARG RCQ NSDL+LMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN
Sbjct: 61  KSPVLCLDGHGIRARGSRCQSNSDLDLMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN 120

Query: 121 LAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQR 180
           LAASTFGEQKLE+LSPGK+TRWRREIEWFLSV DHIVEFVPS Q SKDGI+MEIMVTLQR
Sbjct: 121 LAASTFGEQKLESLSPGKRTRWRREIEWFLSVTDHIVEFVPSKQKSKDGIDMEIMVTLQR 180

Query: 181 KDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNTQRNGDKWWIPAVKVPP 240
           KDLLMNIPALRKLDAMLLGYLDQFGK HEFWYVSRD ESAKD+TQRNGDKWW+PAVKVPP
Sbjct: 181 KDLLMNIPALRKLDAMLLGYLDQFGKLHEFWYVSRDDESAKDDTQRNGDKWWLPAVKVPP 240

Query: 241 NGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYK 300
           NGLS+VTRKWLLFQK+SVNQILKAAMAINAQVLTEMEVP+SYIESLPKNGKTSLGDSIYK
Sbjct: 241 NGLSDVTRKWLLFQKESVNQILKAAMAINAQVLTEMEVPESYIESLPKNGKTSLGDSIYK 300

Query: 301 SITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKR 360
           SITDDYFN ++FLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKR
Sbjct: 301 SITDDYFNPEQFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKR 360

Query: 361 EIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRI 420
           EIFEERVESILFLIKQ FPGISQSALDVSKIQHNKDVGQAILESYSRV+ESLAFNIMSRI
Sbjct: 361 EIFEERVESILFLIKQNFPGISQSALDVSKIQHNKDVGQAILESYSRVIESLAFNIMSRI 420

Query: 421 EDVLYAHTIAREASIEQSFENISW--MRMLSSNGSEALKTLSDFMGWDVDKD-----DLS 480
           EDVLYA TIARE+SIE+S EN+S    + LSS+ S A KTLSDFMGW+VD D      L+
Sbjct: 421 EDVLYADTIARESSIEKSLENLSMDTEKQLSSSDSPASKTLSDFMGWNVDNDSSYIRSLA 480

Query: 481 INESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           I ESCCKEEEDINL+SKP ++          KLESLSALRSPTARH
Sbjct: 481 IKESCCKEEEDINLISKPGNVVTPKRLSYLEKLESLSALRSPTARH 526

BLAST of Tan0005847 vs. NCBI nr
Match: XP_008458416.1 (PREDICTED: rho guanine nucleotide exchange factor 8 [Cucumis melo] >KAA0061550.1 rho guanine nucleotide exchange factor 8 [Cucumis melo var. makuwa] >TYK10722.1 rho guanine nucleotide exchange factor 8 [Cucumis melo var. makuwa])

HSP 1 Score: 834.7 bits (2155), Expect = 4.2e-238
Identity = 437/526 (83.08%), Postives = 473/526 (89.92%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSDN 60
           MVRALI QP++QQSRSF LKR+VELPGRQAHS+ILESGQEG  SRDS EPQ+IGWAL + 
Sbjct: 1   MVRALIQQPSVQQSRSFQLKRVVELPGRQAHSLILESGQEG--SRDSSEPQKIGWALGET 60

Query: 61  NGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN 120
            GPVSCLDAHGIRARG RCQPNSDL+LMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN
Sbjct: 61  KGPVSCLDAHGIRARGHRCQPNSDLDLMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN 120

Query: 121 LAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQR 180
           LAASTFGEQKLE LSPGKK RWRREIEWFLSV DHIVEFVPS Q SKDGI+MEIMVTLQR
Sbjct: 121 LAASTFGEQKLEALSPGKKARWRREIEWFLSVTDHIVEFVPSKQTSKDGIDMEIMVTLQR 180

Query: 181 KDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNTQRNGDKWWIPAVKVPP 240
           KDLLMNIPAL+KLDAMLL YLDQFGK HEFWYVSRD ESAKD+TQRNGDKWW+PAVKVPP
Sbjct: 181 KDLLMNIPALKKLDAMLLSYLDQFGKLHEFWYVSRDDESAKDDTQRNGDKWWLPAVKVPP 240

Query: 241 NGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYK 300
           +GLS+VTRKWLLFQK+SVNQILKAAMAIN+QVLTEMEVP+SYIESLPKNGKTSLGDSIYK
Sbjct: 241 SGLSDVTRKWLLFQKESVNQILKAAMAINSQVLTEMEVPESYIESLPKNGKTSLGDSIYK 300

Query: 301 SITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKR 360
           SITDD+FN ++FLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKD KSSWGS VSFEKR
Sbjct: 301 SITDDHFNPEQFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDLKSSWGSGVSFEKR 360

Query: 361 EIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRI 420
           EIFEERVESILFLIKQ FPGISQSALDVSKIQHNKDVGQAILESYSRV+ESLAFNIMSRI
Sbjct: 361 EIFEERVESILFLIKQKFPGISQSALDVSKIQHNKDVGQAILESYSRVIESLAFNIMSRI 420

Query: 421 EDVLYAHTIAREASIEQSFENISW--MRMLSSNGSEALKTLSDFMGWDVDKD-----DLS 480
           EDVLYA T+ARE+SIE+S EN+     + LSS+ S + KTLSDFMGW+++KD      L+
Sbjct: 421 EDVLYADTVARESSIEESLENLPMHTEKQLSSSDSPSSKTLSDFMGWEIEKDSSYIRSLA 480

Query: 481 INESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           I ESCCK EEDIN ++KP ++          KLESL ALRSPTARH
Sbjct: 481 IKESCCKGEEDIN-VNKPGNVATPKRLSYLEKLESL-ALRSPTARH 522

BLAST of Tan0005847 vs. NCBI nr
Match: XP_011648654.1 (rho guanine nucleotide exchange factor 8 isoform X1 [Cucumis sativus] >KGN60612.1 hypothetical protein Csa_019353 [Cucumis sativus])

HSP 1 Score: 828.6 bits (2139), Expect = 3.0e-236
Identity = 439/527 (83.30%), Postives = 472/527 (89.56%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSDN 60
           MVRALI QP++QQSRSF LKR+VELPGRQAHS+ILESGQEG  SRDS EPQ+IGWAL D 
Sbjct: 1   MVRALIQQPSVQQSRSFQLKRVVELPGRQAHSLILESGQEG--SRDSSEPQKIGWALGDT 60

Query: 61  NGPVSCLDAH-GIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120
            GPVSCLDAH GIRARG RCQPNSDL+LMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT
Sbjct: 61  KGPVSCLDAHGGIRARGLRCQPNSDLDLMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120

Query: 121 NLAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQ 180
           NLAASTFGEQKLE LSPGKK RWRREIEWFLSV DHIVEFVPS Q SKDGI+MEIMVTLQ
Sbjct: 121 NLAASTFGEQKLEALSPGKKARWRREIEWFLSVTDHIVEFVPSKQTSKDGIDMEIMVTLQ 180

Query: 181 RKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNTQRNGDKWWIPAVKVP 240
           RKDLLMNIPALRKLDAMLL YLDQFGK HEFWYVSRD ESAKD+TQRNGDKWW+PAVKVP
Sbjct: 181 RKDLLMNIPALRKLDAMLLSYLDQFGKLHEFWYVSRDDESAKDDTQRNGDKWWLPAVKVP 240

Query: 241 PNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIY 300
           P+GLS+VTRKWLLFQK+SVNQILKAAMAINAQVLTEMEVP+SYIESLPKNGKTSLGDSIY
Sbjct: 241 PSGLSDVTRKWLLFQKESVNQILKAAMAINAQVLTEMEVPESYIESLPKNGKTSLGDSIY 300

Query: 301 KSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEK 360
           +SITDDYFN ++FLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEK
Sbjct: 301 RSITDDYFNPEQFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEK 360

Query: 361 REIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSR 420
           REIFEERVE+ILFLIKQ FPGISQSALDVSKIQH+KDVGQAILESYSRV+ESLAF+IMSR
Sbjct: 361 REIFEERVETILFLIKQKFPGISQSALDVSKIQHSKDVGQAILESYSRVIESLAFSIMSR 420

Query: 421 IEDVLYAHTIAREASIEQSFENISW--MRMLSSNGSEALKTLSDFMGWDVDKD-----DL 480
           IEDVLYA T+ARE+ IE+S EN+S    + LSS+ S + KTLSDFMGW+V+ D      L
Sbjct: 421 IEDVLYADTVARESLIEESLENLSMNTEKRLSSSDSHSSKTLSDFMGWEVENDSSYIRSL 480

Query: 481 SINESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           +I ES CK EEDIN M+KP  +          KLESL ALRSPTARH
Sbjct: 481 AIKESYCKGEEDIN-MNKPATVATPKRLSYLEKLESL-ALRSPTARH 523

BLAST of Tan0005847 vs. NCBI nr
Match: XP_022965672.1 (rho guanine nucleotide exchange factor 8-like [Cucurbita maxima])

HSP 1 Score: 812.8 bits (2098), Expect = 1.7e-231
Identity = 425/528 (80.49%), Postives = 465/528 (88.07%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSDN 60
           MVRA+I+QPN+Q SRSF+LKR+VE+PGRQ HS+ILESG+E  ASR SL+P++IGWALS+N
Sbjct: 1   MVRAVIHQPNLQHSRSFHLKRVVEIPGRQTHSLILESGEESDASRHSLQPEQIGWALSNN 60

Query: 61  N-GPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120
           N  PVSC D  GI+ARGPRCQP S+LELM AKFAKLLLGEDMSGGGKGVSSALALSNAIT
Sbjct: 61  NKDPVSCFDTRGIKARGPRCQPKSELELMMAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120

Query: 121 NLAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQ 180
           NLAASTFGEQKLETLSP KK RWRREIEWFLSV DHIVEFVPS Q SK GI+MEIMVT+Q
Sbjct: 121 NLAASTFGEQKLETLSPEKKARWRREIEWFLSVTDHIVEFVPSKQKSKAGIDMEIMVTVQ 180

Query: 181 RKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNT-QRNGDKWWIPAVKV 240
           RKDLLM+IPAL KLD ML+G+LDQFGKQ EFWYVSRD ESA DNT QRNGDKWW+PAVKV
Sbjct: 181 RKDLLMSIPALTKLDVMLIGFLDQFGKQQEFWYVSRDDESANDNTQQRNGDKWWLPAVKV 240

Query: 241 PPNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSI 300
           PPNGLSE  RKWLLF K+SVNQILKAAMAINAQVLTEMEVP+SYIESLPKNGKTSLG++I
Sbjct: 241 PPNGLSEANRKWLLFVKESVNQILKAAMAINAQVLTEMEVPESYIESLPKNGKTSLGEAI 300

Query: 301 YKSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE 360
           Y+SIT+DYFN DEFLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE
Sbjct: 301 YRSITEDYFNPDEFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE 360

Query: 361 KREIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMS 420
           KREIFEERVESILFLIKQYFPGISQS+LD+SKIQHN+DVGQAILESYSRV+ESLAFN+MS
Sbjct: 361 KREIFEERVESILFLIKQYFPGISQSSLDMSKIQHNRDVGQAILESYSRVIESLAFNMMS 420

Query: 421 RIEDVLYAHTIAREASIEQSFEN--ISWMRMLSSNGSEALKTLSDFMGWDVDKDD----- 480
           RIEDVLYA TIARE SI+QS +N  +       SN SEA KTLSDFMGWDVDKD+     
Sbjct: 421 RIEDVLYADTIARELSIKQSLKNLPVDEEDKSRSNDSEASKTLSDFMGWDVDKDNPNFGS 480

Query: 481 LSINESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           L+  ESC K++EDI+ MSKP  I          KLESLSALRSPTARH
Sbjct: 481 LANEESCWKQQEDIDFMSKPAKIATQKRFSYLEKLESLSALRSPTARH 528

BLAST of Tan0005847 vs. NCBI nr
Match: XP_022938265.1 (rho guanine nucleotide exchange factor 8-like [Cucurbita moschata])

HSP 1 Score: 810.4 bits (2092), Expect = 8.5e-231
Identity = 426/528 (80.68%), Postives = 461/528 (87.31%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSD- 60
           MVRA+I+QPN+Q SRSF+LKR+VE+PGRQ HS+ILESG E   SR SL+P+RIGWALS  
Sbjct: 1   MVRAVIHQPNLQHSRSFHLKRVVEIPGRQTHSLILESGDESDESRHSLQPERIGWALSST 60

Query: 61  NNGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120
           N  PVSC +  GIR RGPRCQP S+LELM AKFAKLLLGEDMSGGGKGVSSALALSNAIT
Sbjct: 61  NKDPVSCFNTPGIRERGPRCQPKSELELMMAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120

Query: 121 NLAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQ 180
           NLAASTFGEQKLETLS  KK RWRREIEWFLSV DHIVEF+PS Q SK GI+MEIMVT+Q
Sbjct: 121 NLAASTFGEQKLETLSSEKKARWRREIEWFLSVTDHIVEFIPSKQKSKAGIDMEIMVTVQ 180

Query: 181 RKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNT-QRNGDKWWIPAVKV 240
           RKDLLM+IPAL KLD ML+G+LDQFGKQ EFWYVSRD ESA DNT QRNGDKWW+PAVKV
Sbjct: 181 RKDLLMSIPALTKLDVMLIGFLDQFGKQQEFWYVSRDDESANDNTQQRNGDKWWLPAVKV 240

Query: 241 PPNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSI 300
           PPNGLSE TRKWLLF K+SVNQILKAAMAINAQVLTEMEVP+SYIESLPKNGKTSLGD+I
Sbjct: 241 PPNGLSEATRKWLLFVKESVNQILKAAMAINAQVLTEMEVPESYIESLPKNGKTSLGDAI 300

Query: 301 YKSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE 360
           Y+SITDDYFN DEFLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE
Sbjct: 301 YRSITDDYFNPDEFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE 360

Query: 361 KREIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMS 420
           KREIFEERVESILFLIKQYFPGISQS+LD+SKIQHNKDVGQAILESYSRV+ESLAFNIMS
Sbjct: 361 KREIFEERVESILFLIKQYFPGISQSSLDMSKIQHNKDVGQAILESYSRVIESLAFNIMS 420

Query: 421 RIEDVLYAHTIAREASIEQSFEN--ISWMRMLSSNGSEALKTLSDFMGWDVDKDD----- 480
           RIEDVLYA TIARE SI+QS +N  +       SN SEA KTLS+FMGWDVDKD+     
Sbjct: 421 RIEDVLYADTIARELSIKQSLKNLPVDEEDKSRSNDSEASKTLSEFMGWDVDKDNPNFGS 480

Query: 481 LSINESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           L+I ESC K++EDI+ MSKP  I          KLESLSALRSPTARH
Sbjct: 481 LAIEESCWKQQEDIDFMSKPAKIATQKRFSYLEKLESLSALRSPTARH 528

BLAST of Tan0005847 vs. ExPASy TrEMBL
Match: A0A5A7V2R1 (Rho guanine nucleotide exchange factor 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00320 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.0e-238
Identity = 437/526 (83.08%), Postives = 473/526 (89.92%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSDN 60
           MVRALI QP++QQSRSF LKR+VELPGRQAHS+ILESGQEG  SRDS EPQ+IGWAL + 
Sbjct: 1   MVRALIQQPSVQQSRSFQLKRVVELPGRQAHSLILESGQEG--SRDSSEPQKIGWALGET 60

Query: 61  NGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN 120
            GPVSCLDAHGIRARG RCQPNSDL+LMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN
Sbjct: 61  KGPVSCLDAHGIRARGHRCQPNSDLDLMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN 120

Query: 121 LAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQR 180
           LAASTFGEQKLE LSPGKK RWRREIEWFLSV DHIVEFVPS Q SKDGI+MEIMVTLQR
Sbjct: 121 LAASTFGEQKLEALSPGKKARWRREIEWFLSVTDHIVEFVPSKQTSKDGIDMEIMVTLQR 180

Query: 181 KDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNTQRNGDKWWIPAVKVPP 240
           KDLLMNIPAL+KLDAMLL YLDQFGK HEFWYVSRD ESAKD+TQRNGDKWW+PAVKVPP
Sbjct: 181 KDLLMNIPALKKLDAMLLSYLDQFGKLHEFWYVSRDDESAKDDTQRNGDKWWLPAVKVPP 240

Query: 241 NGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYK 300
           +GLS+VTRKWLLFQK+SVNQILKAAMAIN+QVLTEMEVP+SYIESLPKNGKTSLGDSIYK
Sbjct: 241 SGLSDVTRKWLLFQKESVNQILKAAMAINSQVLTEMEVPESYIESLPKNGKTSLGDSIYK 300

Query: 301 SITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKR 360
           SITDD+FN ++FLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKD KSSWGS VSFEKR
Sbjct: 301 SITDDHFNPEQFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDLKSSWGSGVSFEKR 360

Query: 361 EIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRI 420
           EIFEERVESILFLIKQ FPGISQSALDVSKIQHNKDVGQAILESYSRV+ESLAFNIMSRI
Sbjct: 361 EIFEERVESILFLIKQKFPGISQSALDVSKIQHNKDVGQAILESYSRVIESLAFNIMSRI 420

Query: 421 EDVLYAHTIAREASIEQSFENISW--MRMLSSNGSEALKTLSDFMGWDVDKD-----DLS 480
           EDVLYA T+ARE+SIE+S EN+     + LSS+ S + KTLSDFMGW+++KD      L+
Sbjct: 421 EDVLYADTVARESSIEESLENLPMHTEKQLSSSDSPSSKTLSDFMGWEIEKDSSYIRSLA 480

Query: 481 INESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           I ESCCK EEDIN ++KP ++          KLESL ALRSPTARH
Sbjct: 481 IKESCCKGEEDIN-VNKPGNVATPKRLSYLEKLESL-ALRSPTARH 522

BLAST of Tan0005847 vs. ExPASy TrEMBL
Match: A0A1S3C7X9 (rho guanine nucleotide exchange factor 8 OS=Cucumis melo OX=3656 GN=LOC103497837 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.0e-238
Identity = 437/526 (83.08%), Postives = 473/526 (89.92%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSDN 60
           MVRALI QP++QQSRSF LKR+VELPGRQAHS+ILESGQEG  SRDS EPQ+IGWAL + 
Sbjct: 1   MVRALIQQPSVQQSRSFQLKRVVELPGRQAHSLILESGQEG--SRDSSEPQKIGWALGET 60

Query: 61  NGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN 120
            GPVSCLDAHGIRARG RCQPNSDL+LMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN
Sbjct: 61  KGPVSCLDAHGIRARGHRCQPNSDLDLMKAKFAKLLLGEDMSGGGKGVSSALALSNAITN 120

Query: 121 LAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQR 180
           LAASTFGEQKLE LSPGKK RWRREIEWFLSV DHIVEFVPS Q SKDGI+MEIMVTLQR
Sbjct: 121 LAASTFGEQKLEALSPGKKARWRREIEWFLSVTDHIVEFVPSKQTSKDGIDMEIMVTLQR 180

Query: 181 KDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNTQRNGDKWWIPAVKVPP 240
           KDLLMNIPAL+KLDAMLL YLDQFGK HEFWYVSRD ESAKD+TQRNGDKWW+PAVKVPP
Sbjct: 181 KDLLMNIPALKKLDAMLLSYLDQFGKLHEFWYVSRDDESAKDDTQRNGDKWWLPAVKVPP 240

Query: 241 NGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYK 300
           +GLS+VTRKWLLFQK+SVNQILKAAMAIN+QVLTEMEVP+SYIESLPKNGKTSLGDSIYK
Sbjct: 241 SGLSDVTRKWLLFQKESVNQILKAAMAINSQVLTEMEVPESYIESLPKNGKTSLGDSIYK 300

Query: 301 SITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKR 360
           SITDD+FN ++FLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKD KSSWGS VSFEKR
Sbjct: 301 SITDDHFNPEQFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDLKSSWGSGVSFEKR 360

Query: 361 EIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRI 420
           EIFEERVESILFLIKQ FPGISQSALDVSKIQHNKDVGQAILESYSRV+ESLAFNIMSRI
Sbjct: 361 EIFEERVESILFLIKQKFPGISQSALDVSKIQHNKDVGQAILESYSRVIESLAFNIMSRI 420

Query: 421 EDVLYAHTIAREASIEQSFENISW--MRMLSSNGSEALKTLSDFMGWDVDKD-----DLS 480
           EDVLYA T+ARE+SIE+S EN+     + LSS+ S + KTLSDFMGW+++KD      L+
Sbjct: 421 EDVLYADTVARESSIEESLENLPMHTEKQLSSSDSPSSKTLSDFMGWEIEKDSSYIRSLA 480

Query: 481 INESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           I ESCCK EEDIN ++KP ++          KLESL ALRSPTARH
Sbjct: 481 IKESCCKGEEDIN-VNKPGNVATPKRLSYLEKLESL-ALRSPTARH 522

BLAST of Tan0005847 vs. ExPASy TrEMBL
Match: A0A0A0LIT3 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G004130 PE=4 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.5e-236
Identity = 439/527 (83.30%), Postives = 472/527 (89.56%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSDN 60
           MVRALI QP++QQSRSF LKR+VELPGRQAHS+ILESGQEG  SRDS EPQ+IGWAL D 
Sbjct: 1   MVRALIQQPSVQQSRSFQLKRVVELPGRQAHSLILESGQEG--SRDSSEPQKIGWALGDT 60

Query: 61  NGPVSCLDAH-GIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120
            GPVSCLDAH GIRARG RCQPNSDL+LMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT
Sbjct: 61  KGPVSCLDAHGGIRARGLRCQPNSDLDLMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120

Query: 121 NLAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQ 180
           NLAASTFGEQKLE LSPGKK RWRREIEWFLSV DHIVEFVPS Q SKDGI+MEIMVTLQ
Sbjct: 121 NLAASTFGEQKLEALSPGKKARWRREIEWFLSVTDHIVEFVPSKQTSKDGIDMEIMVTLQ 180

Query: 181 RKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNTQRNGDKWWIPAVKVP 240
           RKDLLMNIPALRKLDAMLL YLDQFGK HEFWYVSRD ESAKD+TQRNGDKWW+PAVKVP
Sbjct: 181 RKDLLMNIPALRKLDAMLLSYLDQFGKLHEFWYVSRDDESAKDDTQRNGDKWWLPAVKVP 240

Query: 241 PNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIY 300
           P+GLS+VTRKWLLFQK+SVNQILKAAMAINAQVLTEMEVP+SYIESLPKNGKTSLGDSIY
Sbjct: 241 PSGLSDVTRKWLLFQKESVNQILKAAMAINAQVLTEMEVPESYIESLPKNGKTSLGDSIY 300

Query: 301 KSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEK 360
           +SITDDYFN ++FLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEK
Sbjct: 301 RSITDDYFNPEQFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEK 360

Query: 361 REIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSR 420
           REIFEERVE+ILFLIKQ FPGISQSALDVSKIQH+KDVGQAILESYSRV+ESLAF+IMSR
Sbjct: 361 REIFEERVETILFLIKQKFPGISQSALDVSKIQHSKDVGQAILESYSRVIESLAFSIMSR 420

Query: 421 IEDVLYAHTIAREASIEQSFENISW--MRMLSSNGSEALKTLSDFMGWDVDKD-----DL 480
           IEDVLYA T+ARE+ IE+S EN+S    + LSS+ S + KTLSDFMGW+V+ D      L
Sbjct: 421 IEDVLYADTVARESLIEESLENLSMNTEKRLSSSDSHSSKTLSDFMGWEVENDSSYIRSL 480

Query: 481 SINESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           +I ES CK EEDIN M+KP  +          KLESL ALRSPTARH
Sbjct: 481 AIKESYCKGEEDIN-MNKPATVATPKRLSYLEKLESL-ALRSPTARH 523

BLAST of Tan0005847 vs. ExPASy TrEMBL
Match: A0A6J1HKZ0 (rho guanine nucleotide exchange factor 8-like OS=Cucurbita maxima OX=3661 GN=LOC111465501 PE=4 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 8.3e-232
Identity = 425/528 (80.49%), Postives = 465/528 (88.07%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSDN 60
           MVRA+I+QPN+Q SRSF+LKR+VE+PGRQ HS+ILESG+E  ASR SL+P++IGWALS+N
Sbjct: 1   MVRAVIHQPNLQHSRSFHLKRVVEIPGRQTHSLILESGEESDASRHSLQPEQIGWALSNN 60

Query: 61  N-GPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120
           N  PVSC D  GI+ARGPRCQP S+LELM AKFAKLLLGEDMSGGGKGVSSALALSNAIT
Sbjct: 61  NKDPVSCFDTRGIKARGPRCQPKSELELMMAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120

Query: 121 NLAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQ 180
           NLAASTFGEQKLETLSP KK RWRREIEWFLSV DHIVEFVPS Q SK GI+MEIMVT+Q
Sbjct: 121 NLAASTFGEQKLETLSPEKKARWRREIEWFLSVTDHIVEFVPSKQKSKAGIDMEIMVTVQ 180

Query: 181 RKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNT-QRNGDKWWIPAVKV 240
           RKDLLM+IPAL KLD ML+G+LDQFGKQ EFWYVSRD ESA DNT QRNGDKWW+PAVKV
Sbjct: 181 RKDLLMSIPALTKLDVMLIGFLDQFGKQQEFWYVSRDDESANDNTQQRNGDKWWLPAVKV 240

Query: 241 PPNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSI 300
           PPNGLSE  RKWLLF K+SVNQILKAAMAINAQVLTEMEVP+SYIESLPKNGKTSLG++I
Sbjct: 241 PPNGLSEANRKWLLFVKESVNQILKAAMAINAQVLTEMEVPESYIESLPKNGKTSLGEAI 300

Query: 301 YKSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE 360
           Y+SIT+DYFN DEFLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE
Sbjct: 301 YRSITEDYFNPDEFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE 360

Query: 361 KREIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMS 420
           KREIFEERVESILFLIKQYFPGISQS+LD+SKIQHN+DVGQAILESYSRV+ESLAFN+MS
Sbjct: 361 KREIFEERVESILFLIKQYFPGISQSSLDMSKIQHNRDVGQAILESYSRVIESLAFNMMS 420

Query: 421 RIEDVLYAHTIAREASIEQSFEN--ISWMRMLSSNGSEALKTLSDFMGWDVDKDD----- 480
           RIEDVLYA TIARE SI+QS +N  +       SN SEA KTLSDFMGWDVDKD+     
Sbjct: 421 RIEDVLYADTIARELSIKQSLKNLPVDEEDKSRSNDSEASKTLSDFMGWDVDKDNPNFGS 480

Query: 481 LSINESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           L+  ESC K++EDI+ MSKP  I          KLESLSALRSPTARH
Sbjct: 481 LANEESCWKQQEDIDFMSKPAKIATQKRFSYLEKLESLSALRSPTARH 528

BLAST of Tan0005847 vs. ExPASy TrEMBL
Match: A0A6J1FJ98 (rho guanine nucleotide exchange factor 8-like OS=Cucurbita moschata OX=3662 GN=LOC111444404 PE=4 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 4.1e-231
Identity = 426/528 (80.68%), Postives = 461/528 (87.31%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPGRQAHSMILESGQEGHASRDSLEPQRIGWALSD- 60
           MVRA+I+QPN+Q SRSF+LKR+VE+PGRQ HS+ILESG E   SR SL+P+RIGWALS  
Sbjct: 1   MVRAVIHQPNLQHSRSFHLKRVVEIPGRQTHSLILESGDESDESRHSLQPERIGWALSST 60

Query: 61  NNGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120
           N  PVSC +  GIR RGPRCQP S+LELM AKFAKLLLGEDMSGGGKGVSSALALSNAIT
Sbjct: 61  NKDPVSCFNTPGIRERGPRCQPKSELELMMAKFAKLLLGEDMSGGGKGVSSALALSNAIT 120

Query: 121 NLAASTFGEQKLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQ 180
           NLAASTFGEQKLETLS  KK RWRREIEWFLSV DHIVEF+PS Q SK GI+MEIMVT+Q
Sbjct: 121 NLAASTFGEQKLETLSSEKKARWRREIEWFLSVTDHIVEFIPSKQKSKAGIDMEIMVTVQ 180

Query: 181 RKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNT-QRNGDKWWIPAVKV 240
           RKDLLM+IPAL KLD ML+G+LDQFGKQ EFWYVSRD ESA DNT QRNGDKWW+PAVKV
Sbjct: 181 RKDLLMSIPALTKLDVMLIGFLDQFGKQQEFWYVSRDDESANDNTQQRNGDKWWLPAVKV 240

Query: 241 PPNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSI 300
           PPNGLSE TRKWLLF K+SVNQILKAAMAINAQVLTEMEVP+SYIESLPKNGKTSLGD+I
Sbjct: 241 PPNGLSEATRKWLLFVKESVNQILKAAMAINAQVLTEMEVPESYIESLPKNGKTSLGDAI 300

Query: 301 YKSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE 360
           Y+SITDDYFN DEFLKSMDLSTEHKV+DLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE
Sbjct: 301 YRSITDDYFNPDEFLKSMDLSTEHKVIDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFE 360

Query: 361 KREIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMS 420
           KREIFEERVESILFLIKQYFPGISQS+LD+SKIQHNKDVGQAILESYSRV+ESLAFNIMS
Sbjct: 361 KREIFEERVESILFLIKQYFPGISQSSLDMSKIQHNKDVGQAILESYSRVIESLAFNIMS 420

Query: 421 RIEDVLYAHTIAREASIEQSFEN--ISWMRMLSSNGSEALKTLSDFMGWDVDKDD----- 480
           RIEDVLYA TIARE SI+QS +N  +       SN SEA KTLS+FMGWDVDKD+     
Sbjct: 421 RIEDVLYADTIARELSIKQSLKNLPVDEEDKSRSNDSEASKTLSEFMGWDVDKDNPNFGS 480

Query: 481 LSINESCCKEEEDINLMSKPNDI----------KLESLSALRSPTARH 509
           L+I ESC K++EDI+ MSKP  I          KLESLSALRSPTARH
Sbjct: 481 LAIEESCWKQQEDIDFMSKPAKIATQKRFSYLEKLESLSALRSPTARH 528

BLAST of Tan0005847 vs. TAIR 10
Match: AT3G24620.1 (RHO guanyl-nucleotide exchange factor 8 )

HSP 1 Score: 519.2 bits (1336), Expect = 3.6e-147
Identity = 296/534 (55.43%), Postives = 377/534 (70.60%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLKRMVELPG---RQAHSMILESGQEGHASRDSLEPQR----I 60
           MV AL  +  +  S+SFN KRM +      +Q+ ++++E+G       ++ E Q     +
Sbjct: 1   MVAAL--ERGLSASKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESNTPESQNSDSFV 60

Query: 61  GWALSDNNGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALA 120
              +  +   +S L   G R+     +  +D+E+MK +FAKLLLGEDMSGGGKGVSSALA
Sbjct: 61  ESPVESSLPMISPLTRPGKRSE----RQQADMEMMKDRFAKLLLGEDMSGGGKGVSSALA 120

Query: 121 LSNAITNLAASTFGEQ-KLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEM 180
           LSNAITNLAAS FGEQ KL+ +   ++ RW++EI+W LSV DHIVEFVPS Q SKDG+  
Sbjct: 121 LSNAITNLAASIFGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSKDGVCT 180

Query: 181 EIMVTLQRKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKD-NTQRNGDKW 240
           EIMVT QR DLLMNIPALRKLDAML+  LD F   +EFWYVSRD+E  +     R  DKW
Sbjct: 181 EIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDRTNDKW 240

Query: 241 WIPAVKVPPNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGK 300
           W+P VKVPP GLSE +R+ L FQKDSV Q+ KAAMAINAQVL+EME+P+SYI+SLPKNG+
Sbjct: 241 WLPPVKVPPGGLSEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSLPKNGR 300

Query: 301 TSLGDSIYKSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSW 360
            SLGDSIYKSIT+++F+ ++FL  +D+STEHKV+DLKNRIEAS+VIWKRK+H KD KSSW
Sbjct: 301 ASLGDSIYKSITEEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKDTKSSW 360

Query: 361 GSAVSFEKREIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVES 420
           GSAVS EKRE+FEER E+IL L+KQ FPG+ QS+LD+SKIQ NKDVGQA+LESYSR++ES
Sbjct: 361 GSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYSRILES 420

Query: 421 LAFNIMSRIEDVLYAHTIAREASI--------EQSFENISWMRMLSSNGSEA------LK 480
           LA+ +MSRIEDVLY  T+A + ++         ++ E  S     S++G EA       K
Sbjct: 421 LAYTVMSRIEDVLYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSK 480

Query: 481 TLSDFMGWDVDKDDLSINESCCKEEEDINLMSKPNDI--KLESLSALRSPTARH 509
           TL DFMGW+ +      ++   K  +  NL  K      KLE+L+  RSP  RH
Sbjct: 481 TLLDFMGWNDNS-----SKGGDKPTKSPNLTPKKLSYLEKLENLNGFRSPKDRH 523

BLAST of Tan0005847 vs. TAIR 10
Match: AT4G13240.1 (RHO guanyl-nucleotide exchange factor 9 )

HSP 1 Score: 503.8 bits (1296), Expect = 1.6e-142
Identity = 295/529 (55.77%), Postives = 371/529 (70.13%), Query Frame = 0

Query: 11  IQQSRSFNLKRMVE-LPGR-QAHSMILESGQEGHASRDSLEPQRIGWALSDNNGPVSCLD 70
           I  + SFNL RM +  PG+ Q    + E+      ++DSL     G +  + + P++   
Sbjct: 9   ISITSSFNLDRMFDSSPGKEQQQPHLAETTMPESQTQDSL-----GGSPVETSRPMT--- 68

Query: 71  AHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITNLAASTFGE 130
           +  I  R  + Q  S+ E+MK +F KLLLGEDMSGGGKGVSSALALSNAITNLAAS FGE
Sbjct: 69  SRLISRRQDKQQ--SETEMMKDRFTKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGE 128

Query: 131 Q-KLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQRKDLLMNI 190
           Q KL+ ++P ++ RW++EI+W LSV DHIVEFVPS Q SK+G+  EIMVT QR DLLMNI
Sbjct: 129 QTKLQPMAPDRRARWKKEIDWLLSVTDHIVEFVPSQQISKEGVCTEIMVTRQRGDLLMNI 188

Query: 191 PALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKD-NTQRNGDKWWIPAVKVPPNGLSEV 250
           PALRKLDAML+  LD F   +EFWYVSRD+E  K    +R  DKWW+P VKVPPNGLSE 
Sbjct: 189 PALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGKQARNERTKDKWWLPPVKVPPNGLSES 248

Query: 251 TRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYKSITDDY 310
            R+ L FQKDSV+Q+ KAAMAINAQVL+EM +PDSYIESLPKNG+ SLGDS+YKSIT+++
Sbjct: 249 ARRMLHFQKDSVSQVQKAAMAINAQVLSEMAIPDSYIESLPKNGRVSLGDSLYKSITEEW 308

Query: 311 FNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKREIFEER 370
           F+ ++FL ++DLSTEHKV+D+KNRIEASIVIWKRK+H KD KSSWGSAVS EKRE+FEER
Sbjct: 309 FDPEQFLSTLDLSTEHKVLDVKNRIEASIVIWKRKLHLKDNKSSWGSAVSLEKRELFEER 368

Query: 371 VESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRIEDVLYA 430
            E+IL L+KQ FPG+ QS+LD+SKIQ+NKDVG A+LESYSR++ESL +  MSRI+DVLYA
Sbjct: 369 AETILVLLKQKFPGLPQSSLDISKIQYNKDVGHAVLESYSRILESLGYTEMSRIDDVLYA 428

Query: 431 HTIARE----------------------ASIEQSFENISWMRMLSSNGSEALKTLSDFMG 490
            ++AR+                       S   S E I   ++ S N S+   TL DF+G
Sbjct: 429 DSLARKQCTGEETSDGGKIATETDSASAGSSNYSGEEIE--KLESQNSSKT--TLLDFIG 488

Query: 491 WDVDKDDLSINESCCKEEEDINLMSKPNDI----KLESLSALRSPTARH 509
           W    D+ S  +S   E+   +    P  +    KLE+L+  RSP  RH
Sbjct: 489 W---SDNSSKGQS---EKPPKSPRMTPKKLSYLEKLENLNGFRSPKDRH 517

BLAST of Tan0005847 vs. TAIR 10
Match: AT1G79860.1 (RHO guanyl-nucleotide exchange factor 12 )

HSP 1 Score: 481.1 bits (1237), Expect = 1.1e-135
Identity = 281/530 (53.02%), Postives = 361/530 (68.11%), Query Frame = 0

Query: 1   MVRALIYQPNIQQSRSFNLK---RMVELPGRQAHSMILESG-QEGHASRDSLEPQRI--- 60
           MVRA   +    +SR FN K          R   S+ +E+G  E        EP  I   
Sbjct: 1   MVRASEQEQETYRSRLFNFKWRNNDNNSATRHNKSLSVETGLDEAATGSHDAEPLTIIHP 60

Query: 61  --GWALSDNNGPVSCLDAHGIRARGPRCQPNSDLELMKAKFAKLLLGEDMSGGGKGVSSA 120
             G  LS +    + L A        R Q  +D+E MK +F+KLLLGED SGGGKGVSSA
Sbjct: 61  SQGPPLSRSAADEAVLAALAASQARER-QLLADMEQMKERFSKLLLGEDNSGGGKGVSSA 120

Query: 121 LALSNAITNLAASTFGEQ-KLETLSPGKKTRWRREIEWFLSVADHIVEFVPSIQ-SKDGI 180
           LALSNAITNLAAS FGEQ +LE +   ++ RWR+EI+W LSV D++VEF PS Q +KDG 
Sbjct: 121 LALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLSVTDYVVEFAPSQQKNKDGT 180

Query: 181 EMEIMVTLQRKDLLMNIPALRKLDAMLLGYLDQFGKQHEFWYVSRDAESAKDNTQRNGDK 240
            MEIM T QR DL MNIPAL+KLDAML+  L+ F  Q EF Y+S+D+     + +RN +K
Sbjct: 181 NMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFSYISKDSPDL--DGKRNDEK 240

Query: 241 WWIPAVKVPPNGLSEVTRKWLLFQKDSVNQILKAAMAINAQVLTEMEVPDSYIESLPKNG 300
           WWIP VKVPP+GLSE +R++L +QKD VNQ+LKAAMAINAQVL EME+P+SYI+SLPKNG
Sbjct: 241 WWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINAQVLFEMEIPESYIDSLPKNG 300

Query: 301 KTSLGDSIYKSITDDYFNTDEFLKSMDLSTEHKVVDLKNRIEASIVIWKRKMHHKDGKSS 360
           + SLGD +YK+IT D+F+ D+FL SMD+S+EHK+VDLKNRIEASI+IWKRKM +KD KSS
Sbjct: 301 RASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNRIEASIIIWKRKMVYKDNKSS 360

Query: 361 --WGSAVSFEKREIFEERVESILFLIKQYFPGISQSALDVSKIQHNKDVGQAILESYSRV 420
             W S VS EKRE+FEER E+IL ++KQ +PGISQS+LD+SKIQ N+DVGQA+LESYSR+
Sbjct: 361 APWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDISKIQFNEDVGQAVLESYSRI 420

Query: 421 VESLAFNIMSRIEDVLYAHTIAREASIEQSFENISWMRMLSSNGSEALKTLSDFMGWDVD 480
           +ESLA+ ++SRI+DVL A     + +     E  + +  +         TLSDFMGWD D
Sbjct: 421 LESLAYTVLSRIDDVLEADRAGNKRNTPLEAEEETLVGSM---------TLSDFMGWDFD 480

Query: 481 ---------KDDLSINESCCKEEEDINLMSKPNDIKLESLSALRSPTARH 509
                    K DL  ++   KE+  +++++      LE+L  ++SPTARH
Sbjct: 481 QAANAELESKKDLP-DDPLIKEK--LSVVTTKKTSYLETLGGVKSPTARH 515

BLAST of Tan0005847 vs. TAIR 10
Match: AT1G52240.1 (RHO guanyl-nucleotide exchange factor 11 )

HSP 1 Score: 479.6 bits (1233), Expect = 3.2e-135
Identity = 270/442 (61.09%), Postives = 330/442 (74.66%), Query Frame = 0

Query: 85  LELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITNLAASTFGEQ-KLETLSPGKKTRWR 144
           +E MK KF+KLLLGEDMSGGGKGVSSALALSNAITNLAAS FGEQ +LE ++  +KTRWR
Sbjct: 169 MEQMKEKFSKLLLGEDMSGGGKGVSSALALSNAITNLAASAFGEQRRLEPMAVDRKTRWR 228

Query: 145 REIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQRKDLLMNIPALRKLDAMLLGYLDQ 204
           REI W +SVAD+IVEF P+ Q +KDG  ME+M T QR DLL NIPAL+KLDAMLL  LD+
Sbjct: 229 REIGWLISVADYIVEFAPTQQTNKDGTSMEVMSTRQRTDLLCNIPALKKLDAMLLDCLDK 288

Query: 205 FGKQHEFWYVSRDAESAKDNTQRNGDKWWIPAVKVPPNGLSEVTRKWLLFQKDSVNQILK 264
           F  Q EF+YV +D+  + +   RN +KWW+PAVKVPPNGLSE++R++L  QK+ VNQ+LK
Sbjct: 289 FKDQDEFYYVKKDSPDSCET--RNDEKWWLPAVKVPPNGLSEISRRFLQSQKECVNQVLK 348

Query: 265 AAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYKSITDDYFNTDEFLKSMDLSTEHK 324
           AAMAINAQVL+EME+P+SY+ESLPKNG+ SLGD IY+ IT + F+ D+FL  MDLS+EHK
Sbjct: 349 AAMAINAQVLSEMEIPESYLESLPKNGRASLGDVIYRMITVEMFDADQFLIEMDLSSEHK 408

Query: 325 VVDLKNRIEASIVIWKRKMHHKDGKSSWGSAVSFEKREIFEERVESILFLIKQYFPGISQ 384
           ++DLKNRIEASIVIWKRKM  KD KS WGS VS EKRE FEER E+IL L+KQ FPGISQ
Sbjct: 409 ILDLKNRIEASIVIWKRKMVQKDTKSPWGSTVSIEKREQFEERAETILLLLKQGFPGISQ 468

Query: 385 SALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRIEDVLYAHTIAREASIEQSFENIS 444
           S+LD+SKIQ N+DVG AILESYSRV+ESLA  +MSRIEDVLYA  + +E +     +N  
Sbjct: 469 SSLDISKIQFNRDVGLAILESYSRVLESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRY 528

Query: 445 WMR--------MLSSNGSEALKTLSDFMGWDVDKDDLSINESCCKEEEDINLMSKPNDIK 504
            ++         LS     A  TLSD M W  +K++    ES   + E   L+SK   I 
Sbjct: 529 SLKENEKLREERLSFTEDMASGTLSDVMQWG-NKNNEMKKESFFGDREK-PLLSKVTGIM 588

Query: 505 --------LESLSALRSPTARH 509
                   L++L A+RSPTAR+
Sbjct: 589 TNNKKSSYLDNLGAMRSPTARY 606

BLAST of Tan0005847 vs. TAIR 10
Match: AT5G19560.1 (ROP uanine nucleotide exchange factor 10 )

HSP 1 Score: 450.7 bits (1158), Expect = 1.6e-126
Identity = 257/454 (56.61%), Postives = 315/454 (69.38%), Query Frame = 0

Query: 83  SDLELMKAKFAKLLLGEDMSGGGKGVSSALALSNAITNLAASTFGEQ-KLETLSPGKKTR 142
           SD+E+MK +FAKLLLGEDMSGGG G +SALALSNAIT LA S FGEQ KL+ + P  K  
Sbjct: 43  SDMEVMKERFAKLLLGEDMSGGGTGETSALALSNAITKLADSMFGEQMKLQPMYPETKEN 102

Query: 143 WRREIEWFLSVADHIVEFVPSIQ-SKDGIEMEIMVTLQRKDLLMNIPALRKLDAMLLGYL 202
           WR+E+ W LSV DHIV+FVPS Q  K+G   EIMVT QR DLL NIPALRKLD++LL  L
Sbjct: 103 WRKEMGWLLSVIDHIVQFVPSRQMGKNGQFTEIMVTKQRDDLLTNIPALRKLDSVLLETL 162

Query: 203 DQFGKQHEFWYVSRDAESAKDNTQ-RNGDKWWIPAVKVPPNGLSEVTRKWLLFQKDSVNQ 262
           D F  Q +FWYV RD E A  N   R  + WW+P VKVP +GLSE +R+WL  QKDSV Q
Sbjct: 163 DNFKDQKDFWYVPRDMEDADHNGDWRRDENWWLPVVKVPTDGLSEESRRWLQNQKDSVAQ 222

Query: 263 ILKAAMAINAQVLTEMEVPDSYIESLPKNGKTSLGDSIYKSITDDYFNTDEFLKSMDLST 322
           +LKAA AINA VL+EM VP++YI+SLPKNGKTSLGD +YKSIT++ F+ D F+  +DLST
Sbjct: 223 VLKAATAINAHVLSEMHVPENYIDSLPKNGKTSLGDFLYKSITEESFDPDYFVSFLDLST 282

Query: 323 EHKVVDLKNRIEASIVIWKRKM--HHKDGKSSWGSAVSFEKREIFEERVESILFLIKQYF 382
           EHKV+DLKNRIEAS+VIWKRKM    KDGKS WGS VS EKRE+FE R E+IL ++KQ F
Sbjct: 283 EHKVLDLKNRIEASMVIWKRKMCQKEKDGKSQWGSTVSLEKRELFEVRAETILVMLKQQF 342

Query: 383 PGISQSALDVSKIQHNKDVGQAILESYSRVVESLAFNIMSRIEDVLYAHTIAR------- 442
           PGI QS+L+VSKI++NKDVGQAILESYSRV+ESLA  IMSRIEDVL A  + +       
Sbjct: 343 PGIPQSSLEVSKIKNNKDVGQAILESYSRVLESLASKIMSRIEDVLEADRLVQRQLMGEA 402

Query: 443 ----EASIEQSFENISWMRMLSSNGSEALKTLSDFMGWDVDKDDLSINESC-------CK 502
               E+  E  +E    +    +  S   + LSDF+GW +  D    +           +
Sbjct: 403 ETRSESEAESEYEETEKVVAAETPNS---RKLSDFIGWRLSSDTKKHSSMSDIEFFHKVE 462

Query: 503 EEEDINLMSKPNDIK-----LESLSALRSPTARH 509
           +E++  +M  P  +      L  L  +RSP+ RH
Sbjct: 463 QEKEKPMMKSPRALPKKFSYLAKLENMRSPSDRH 493