Tan0005303 (gene) Snake gourd v1

BLAST of Tan0005303 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 1.3e-196
Identity = 355/624 (56.89%), Postives = 448/624 (71.79%), Query Frame = 0

Query: 2   GDGIVETALSSLGKGFDLTSDFRLKYCK------GEQRLVLLNEAQRRELIVPGFGAVQD 61
           G  ++ETA+ SLGKGFDLT+DFRLKYCK      G+ RLV+L++ Q REL +PGFG  Q+
Sbjct: 5   GGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQN 64

Query: 62  VSIDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAV 121
           VS DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FN  FGFQSG WA DA 
Sbjct: 65  VSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAA 124

Query: 122 NTKCLGLDGYFISLFNAHI-DRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLS 181
           N K LGLD   ++LFN HI +   LRL+D VR+AVPSSWDP  +ARFIE+YGTH+I G+S
Sbjct: 125 NVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVS 184

Query: 182 IGGHDVVLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHH---KTKTPQ 241
           +GG DVV+VRQDKSS L+   L++HL +LGDQLFTG+C           HH   + K P+
Sbjct: 185 VGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPE 244

Query: 242 AFNLF-DPQLMAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFI 301
           AFN+F D Q +AF NF S+++++GI VI +KRGG   A SH EWL TV + PDAI+F FI
Sbjct: 245 AFNVFDDKQTVAFNNF-SINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 304

Query: 302 PIISLLEGFPGKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNIT 361
           PI SLL+  PG G LSHA++LYLRYKPP+ DLQ+FLDF   + WAP+HNDLP G  PN+ 
Sbjct: 305 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 364

Query: 362 TS-PSLQLNLMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLEN 421
           ++ P+L +N MGPKLYVNTT VT+E  PVTGMR +LEG K NRLAIH+QHL  T   +  
Sbjct: 365 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 424

Query: 422 NITQQSPPLWHSSQHIA-GDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPA 481
            IT +   +W  S  I   D Y EPLN  KFS++ T PVKYDP+W  ++S+    + +  
Sbjct: 425 KITDEH--IWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHK--SQNDV 484

Query: 482 AFIVTGAQLHVIKQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTS 541
           AFIVTGAQL V K+  SK++LHLRL Y+KVS+ YVVQ +W       S KSGIFS++S  
Sbjct: 485 AFIVTGAQLEV-KKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMP 544

Query: 542 L-SAATASTPKEKPPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHW 601
           L S +      +K  N   V++DSGVF GGPPVP    K+VKFV+LS++ RGPQ SPGHW
Sbjct: 545 LTSGSVHHNMIQKDKN--EVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHW 604

Query: 602 VVIGARLNLEKGKISLHVKFGLLH 612
           +V G RL L+KGK+ LHVKF LLH
Sbjct: 605 LVTGVRLYLDKGKLCLHVKFALLH 619

BLAST of Tan0005303 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 2.0e-139
Identity = 278/615 (45.20%), Postives = 383/615 (62.28%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCKG---EQRLVLLNEAQRR-ELIVPGFGAVQDVSIDIKC 66
           E A+ S+G G+DL  D RLKYCKG   + RL+ + E     E+++PG  ++ +VS  IKC
Sbjct: 12  EVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKC 71

Query: 67  DRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCLGL 126
           D+G+R R++SDIL F QM+E FN++ S+ GKIPSG FN MF F S CW  DA  TK L  
Sbjct: 72  DKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYTKNLAF 131

Query: 127 DGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVL 186
           DG FISL++  +D+  + L + V+ AVPS+WDP A+ARFI+ YGTHIIV + +GG DV+ 
Sbjct: 132 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 191

Query: 187 VRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLS-KTKDHHKTKTPQAFNLFDPQLM 246
            +Q  SS L+P +L+  L+E+ D+ F  A       S + +   K +T +       Q +
Sbjct: 192 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE-------QRL 251

Query: 247 AFGNFSSVSA---KDGIIVITSKRGGSATAS-SHCEWLPTVLESPDAIHFQFIPIISLLE 306
            F + SS+ +   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL 
Sbjct: 252 RFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLN 311

Query: 307 GFPGKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQL 366
           G PG GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +  SLQ 
Sbjct: 312 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQF 371

Query: 367 NLMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLL--LENNITQQS 426
           +  GPKLYVNTT V    +P+TGMRLYLEG +SNRLAIH+QHL + P +  LE+++ +  
Sbjct: 372 SFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI 431

Query: 427 PPLWHSSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGA 486
               H  +      Y E +N   +S++ T PV+ D   +                +VTGA
Sbjct: 432 RQESHDRR------YYEKVNWKNYSHVCTEPVESDDDLS----------------VVTGA 491

Query: 487 QLHVIKQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSA-ISTSLSAATA 546
           QLHV +    K +L LRL +S+V  + +V+ +    +   +PKSG+ S  IS   +AA  
Sbjct: 492 QLHV-ESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAA-- 551

Query: 547 STPKEKPPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARL 606
              ++ PP  A V ++S ++ GGPPVP QA KL+KFV+ SEM RGPQ+SPG+WVV GARL
Sbjct: 552 ---QKPPPRPADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARL 590

Query: 607 NLEKGKISLHVKFGL 610
            +EKGKISL VK+ L
Sbjct: 612 LVEKGKISLKVKYSL 590

BLAST of Tan0005303 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 1.0e-132
Identity = 272/619 (43.94%), Postives = 373/619 (60.26%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCK---GEQRLVLLNEAQRRELIVPGFGAVQDVSIDIKCD 66
           E A+S +G G+DL SD R   CK      RLV ++  + R+LI PG   V +VS  IKCD
Sbjct: 16  EKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 75

Query: 67  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCLGLD 126
           +G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN MF F S CW  DA + K L  D
Sbjct: 76  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSVKTLAYD 135

Query: 127 GYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 186
           G+FISL++  I R  L L DEV+  VPSSWD  A+A FIEKYGTH++VG+++GG DV+ V
Sbjct: 136 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 195

Query: 187 RQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQLMAF 246
           +Q + S+ EP E++  L+  GD+ F       P  SK+     +  P+  NL    L  F
Sbjct: 196 KQMRKSNHEPEEIQKMLKHWGDERF----CVDPVESKSPASVYSGKPKEENLLQWGLQPF 255

Query: 247 GNFSSVSA-----KDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEG 306
           G   S +       + I+ +  +RGG     SH  WL TV ++P+ I   F+PI SLL G
Sbjct: 256 GTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSG 315

Query: 307 FPGKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLN 366
            PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +SPSLQ +
Sbjct: 316 LPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFS 375

Query: 367 LMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPL 426
           LMGPKLYVNT++V +  +PVTG+R +LEG K N LAIH+QHL   P  L  +      P+
Sbjct: 376 LMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPI 435

Query: 427 WHSSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLH 486
               +     GY  P+    FS++ T PV+Y           +G  +   A IVT A L 
Sbjct: 436 ----EEPVEKGYYVPVKWGIFSHVCTYPVQY-----------NGARSDDTASIVTKAWLE 495

Query: 487 VIKQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPK 546
           V K    + +L LRL +S  +++   ++ W   S++ S KSG+FS IST LS   +  P 
Sbjct: 496 V-KGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRKSGVFSMISTRLSTGLSPNPA 555

Query: 547 EKPPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEK 606
              P  + + ++S V+  GP  P++  KL+  V+  E+ RGP++ PG+WVV GA+L +E 
Sbjct: 556 TTKPQ-SKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEA 610

Query: 607 GKISLHVKFGLLHVVSETS 618
           GKIS+  K+ LL V+SE S
Sbjct: 616 GKISIKAKYSLLTVISEDS 610

BLAST of Tan0005303 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 1.9e-110
Identity = 240/612 (39.22%), Postives = 342/612 (55.88%), Query Frame = 0

Query: 6   VETALSSLGKGFDLTSDFRLKYCKGE--QRLVLLNEAQRRELIVPGFGAVQDVSIDIKCD 65
           +  A+ +LG+GFD+TSD RL YCKG    RLV + E Q R+L +     + +V  DI C 
Sbjct: 21  LRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCS 80

Query: 66  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCLGLD 125
           RG+    +  + +F++M+E FN +  + G IP G FN MF + +G W +DA +TK L L 
Sbjct: 81  RGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALV 140

Query: 126 GYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 185
           GYFI L++  + +  L L +E+R AVPSSWDP ++A FIE YGTHI+  ++IGG DVV +
Sbjct: 141 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 200

Query: 186 RQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQLMAF 245
           RQ +SS L  SE++N++ ++                     H+    ++ ++  P     
Sbjct: 201 RQHQSSPLPVSEIENYVNDM-------------------IKHRFHEAESQSITGP----- 260

Query: 246 GNFSSVSAKD-GIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFPGK 305
                +  KD  I VI  +RGG     SH  W  TV  +PD I+  F PI+SLLEG PG 
Sbjct: 261 -----LKYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGL 320

Query: 306 GFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLMGP 365
             L+ AI LYL YKPPI DLQ+FLD+Q+ + WAP  ++L            SLQ +LMGP
Sbjct: 321 RHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGP 380

Query: 366 KLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSP---PLW 425
           KL+++  QVT   KPVTG+RL LEG K NRL+IH+QHL++ P +L+ +     P   P W
Sbjct: 381 KLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKW 440

Query: 426 HSSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHV 485
              +      + EP+    FS++ST+P+++       + +  GD +     IVTGAQL V
Sbjct: 441 QGPEE-QDSRWFEPIKWKNFSHVSTSPIEH-------TETHIGDLS--GVHIVTGAQLGV 500

Query: 486 IKQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKE 545
                SK +LHL+LL+SKV    + ++ W     D +P                AS+ + 
Sbjct: 501 -WDFGSKNVLHLKLLFSKVPGCTIRRSVW-----DHTP---------------VASSGRL 560

Query: 546 KPPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKG 605
           +P   +T             V  Q+ KL K V+ SEM +GPQD PGHW+V GA+L +EKG
Sbjct: 561 EPGGPST-------SSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKG 560

Query: 606 KISLHVKFGLLH 612
           KI L VK+ LL+
Sbjct: 621 KIVLRVKYSLLN 560

BLAST of Tan0005303 vs. NCBI nr
Match: XP_022990956.1 (MACPF domain-containing protein At1g14780 [Cucurbita maxima])

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 543/621 (87.44%), Postives = 585/621 (94.20%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLN+AQRR+LIVPGFGAV+D+SIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN MFGFQSGCWAMDA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVP+SWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS+LEPSELK+HLEELGDQLFTGACTFPPP SK KDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +A+ NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+  P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQ+HKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ +PPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SS H+A D Y+EPLNRNKFSY+STAPVKYDP+WA ++++      +PAAFIVTGAQLHV+
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATP-----TPAAFIVTGAQLHVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           K  DSKA LHL LLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLS  +AS PK+K
Sbjct: 481 KHHDSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLS-GSASAPKKK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP+ ATVIVDSG+FH GPPVPLQAQKLVKFVELSEMR+GPQDSPGHWVVIGARLNLE GK
Sbjct: 541 PPDPATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I LHVKFGL+HVVSETSGD+Q
Sbjct: 601 ICLHVKFGLVHVVSETSGDEQ 615

BLAST of Tan0005303 vs. NCBI nr
Match: XP_022952953.1 (MACPF domain-containing protein At1g14780 [Cucurbita moschata] >XP_022952961.1 MACPF domain-containing protein At1g14780 [Cucurbita moschata])

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 543/621 (87.44%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLNEAQRR+LIVPGFG V+DVSIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRRDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FN MFGFQSGCWAMDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS+LE SELK+HLEELGDQLFTGACTFPPP SK KDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +A+ NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+  P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQVHKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ +PPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SS H+A D Y+EPLNRNKFSY+STAPV YDP+WA ++        +PAAFIVTGAQLHV+
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTWATATP-------TPAAFIVTGAQLHVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           K  DSKA LHL LLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSLS A +S PKEK
Sbjct: 481 KHHDSKAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLSGA-SSAPKEK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP+ ATVIVDSGVFH GPPVPLQAQKLVKFVELSEMR+GPQDSPGHWVVIGARLNLE  K
Sbjct: 541 PPDPATVIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I LHVKFGL+HVVSETS D+Q
Sbjct: 601 ICLHVKFGLVHVVSETSADEQ 613

BLAST of Tan0005303 vs. NCBI nr
Match: XP_023546039.1 (MACPF domain-containing protein At1g14780 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 543/621 (87.44%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLNEAQRR+LIVPGFGA++DVSIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNEAQRRDLIVPGFGAIKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FN MFGFQSGCWAMDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVPSSWDPPAIARFIEKYGTHIIVGLSIGG DV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGQDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS+L+PSELK+HLEELGDQLFTGACTFPPP SK KDHHKTK PQAFNLFD +L
Sbjct: 181 VLVRQDKSSNLQPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDTKL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +A+ NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+  P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQVHKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQVT+E KPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ +PPLWH
Sbjct: 361 GPKLYVNTTQVTSEKKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SS H+A D Y+EPLNRNKFSY+STAPVKYDP+WA ++        +PAAFIVTGAQLHV+
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATP-------TPAAFIVTGAQLHVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           K  DSKA LHL LLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLS AT S PKEK
Sbjct: 481 KHHDSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSGAT-SAPKEK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP+ ATVIVDSGVFH GPPVPLQAQKLVKFVELSEMR+GPQDSPGHWVVIGARLNLE  K
Sbjct: 541 PPDPATVIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I LHVKFGL+HVVSETS D+Q
Sbjct: 601 ICLHVKFGLVHVVSETSADEQ 613

BLAST of Tan0005303 vs. NCBI nr
Match: KAG7032583.1 (MACPF domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1113.6 bits (2879), Expect = 0.0e+00
Identity = 542/621 (87.28%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLNEAQRR+LIVPGFG V+DVSIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRRDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FN +FGFQSGCWAMDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAIFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS+LE SELK+HLEELGDQLFTGACTFPPP SK KDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +A+ NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+  P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQVHKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ +PPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SS H+A D Y+EPLNRNKFSY+STAPV YDP+WA ++        +PAAFIVTGAQLHV+
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTWATATP-------TPAAFIVTGAQLHVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           K  DSKA LHL LLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSLS A +S PKEK
Sbjct: 481 KHHDSKAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLSGA-SSAPKEK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP+ ATVIVDSGVFH GPPVPLQAQKLVKFVELSEMR+GPQDSPGHWVVIGARLNLE  K
Sbjct: 541 PPDPATVIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I LHVKFGL+HVVSETS D+Q
Sbjct: 601 ICLHVKFGLVHVVSETSADEQ 613

BLAST of Tan0005303 vs. NCBI nr
Match: KAG6601885.1 (MACPF domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 541/621 (87.12%), Postives = 577/621 (92.91%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLNEAQR +LIVPGFG V+DVSIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRPDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FN MFGFQ+GCWAMDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQTGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS+LE SELK+HLEELGDQLFTGACTFPPP SK KDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +A+ NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+  P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQVHKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ +PPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SS H+A D Y+EPLNRNKFSY+STAPV YDP+WA ++        +PAAFIVTGAQLHV+
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTWATATP-------TPAAFIVTGAQLHVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           K  DSKA LHL LLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSLS A +S PKEK
Sbjct: 481 KHHDSKAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLSGA-SSAPKEK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP+ ATVIVDSGVFH GPPVPLQAQKLVKFVELSEMR+GPQDSPGHWVVIGARLNLE  K
Sbjct: 541 PPDPATVIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I LHVKFGL+HVVSETS D+Q
Sbjct: 601 ICLHVKFGLVHVVSETSADEQ 613

BLAST of Tan0005303 vs. ExPASy TrEMBL
Match: A0A6J1JPD7 (MACPF domain-containing protein At1g14780 OS=Cucurbita maxima OX=3661 GN=LOC111487690 PE=4 SV=1)

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 543/621 (87.44%), Postives = 585/621 (94.20%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLN+AQRR+LIVPGFGAV+D+SIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGQHRLVLLNQAQRRDLIVPGFGAVKDISIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN MFGFQSGCWAMDA NTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNAMFGFQSGCWAMDAENTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVP+SWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPTSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS+LEPSELK+HLEELGDQLFTGACTFPPP SK KDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEPSELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +A+ NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+  P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQ+HKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQMHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ +PPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SS H+A D Y+EPLNRNKFSY+STAPVKYDP+WA ++++      +PAAFIVTGAQLHV+
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVKYDPTWATATATP-----TPAAFIVTGAQLHVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           K  DSKA LHL LLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLS  +AS PK+K
Sbjct: 481 KHHDSKAALHLGLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLS-GSASAPKKK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP+ ATVIVDSG+FH GPPVPLQAQKLVKFVELSEMR+GPQDSPGHWVVIGARLNLE GK
Sbjct: 541 PPDPATVIVDSGIFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENGK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I LHVKFGL+HVVSETSGD+Q
Sbjct: 601 ICLHVKFGLVHVVSETSGDEQ 615

BLAST of Tan0005303 vs. ExPASy TrEMBL
Match: A0A6J1GM16 (MACPF domain-containing protein At1g14780 OS=Cucurbita moschata OX=3662 GN=LOC111455473 PE=4 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 543/621 (87.44%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           MGDGIV+ AL+SLGKGFDLTSDF+LKYCKG+ RLVLLNEAQRR+LIVPGFG V+DVSIDI
Sbjct: 1   MGDGIVDAALASLGKGFDLTSDFKLKYCKGKHRLVLLNEAQRRDLIVPGFGPVKDVSIDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSG FN MFGFQSGCWAMDAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGFFNAMFGFQSGCWAMDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVR+AVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRAAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS+LE SELK+HLEELGDQLFTGACTFPPP SK KDHHKTK PQAFNLFDP+L
Sbjct: 181 VLVRQDKSSNLEASELKSHLEELGDQLFTGACTFPPPHSKPKDHHKTKLPQAFNLFDPKL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +A+ NFSSVSAKDGIIVITSK+GGS TAS+HCEWLPTVLESPDAIHFQFIPIISLL+  P
Sbjct: 241 VAYANFSSVSAKDGIIVITSKKGGSPTASTHCEWLPTVLESPDAIHFQFIPIISLLDRVP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQVHKIWAPIHNDLPLGPTPNITTSP LQ NLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNITTSPYLQPNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQVT+ENKPVTG+RLYLEGIKSNRLAIHIQHL TTP+LL+NNITQ +PPLWH
Sbjct: 361 GPKLYVNTTQVTSENKPVTGLRLYLEGIKSNRLAIHIQHLQTTPILLQNNITQHNPPLWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SS H+A D Y+EPLNRNKFSY+STAPV YDP+WA ++        +PAAFIVTGAQLHV+
Sbjct: 421 SSLHVADDRYVEPLNRNKFSYVSTAPVTYDPTWATATP-------TPAAFIVTGAQLHVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           K  DSKA LHL LLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSLS A +S PKEK
Sbjct: 481 KHHDSKAALHLGLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLSGA-SSAPKEK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP+ ATVIVDSGVFH GPPVPLQAQKLVKFVELSEMR+GPQDSPGHWVVIGARLNLE  K
Sbjct: 541 PPDPATVIVDSGVFHHGPPVPLQAQKLVKFVELSEMRKGPQDSPGHWVVIGARLNLENSK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I LHVKFGL+HVVSETS D+Q
Sbjct: 601 ICLHVKFGLVHVVSETSADEQ 613

BLAST of Tan0005303 vs. ExPASy TrEMBL
Match: A0A5D3CRV5 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold663G00330 PE=4 SV=1)

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 546/621 (87.92%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           M D IV+ AL+SLGKGFDLTSDFRLKYCKG   LVLLN++QRR+LIVPGFGAV+DVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWA DAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPS+WDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS LEPSELKNHLEELGDQLF GACTFPPPLSK KDHHK K PQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +AFGNFSSVSAKDGIIVITSKRGGS TASSHCEWLPTV ESPDAIHFQFIPIISLLEG P
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQVHKIWAPIHNDLPLGPTPNI+TSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQV A+NKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQ+   WH
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SSQHIA D Y+EPLNRNKFS++ T P+KYDP+WA    +TSGD NSPAAFIVTGAQL V+
Sbjct: 421 SSQHIADDRYVEPLNRNKFSHVCTVPIKYDPAWA----TTSGDDNSPAAFIVTGAQLLVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           KQ DSK +LHLRLLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSL+ A+A+  KEK
Sbjct: 481 KQHDSKTVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAAL-KEK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP AA VIVDSGVFHGGPP+ +Q QKLVKFVELSEMR+GPQDSPG+W+VIGARLNLEKGK
Sbjct: 541 PPEAA-VIVDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I L VKFGLLHV+SET  D Q
Sbjct: 601 ICLQVKFGLLHVLSETITDDQ 613

BLAST of Tan0005303 vs. ExPASy TrEMBL
Match: E5GB62 (MACPF domain-containing protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 546/621 (87.92%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           M D IV+ AL+SLGKGFDLTSDFRLKYCKG   LVLLN++QRR+LIVPGFGAV+DVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWA DAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPS+WDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS LEPSELKNHLEELGDQLF GACTFPPPLSK KDHHK K PQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +AFGNFSSVSAKDGIIVITSKRGGS TASSHCEWLPTV ESPDAIHFQFIPIISLLEG P
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQVHKIWAPIHNDLPLGPTPNI+TSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQV A+NKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQ+   WH
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SSQHIA D Y+EPLNRNKFS++ T P+KYDP+WA    +TSGD NSPAAFIVTGAQL V+
Sbjct: 421 SSQHIADDRYVEPLNRNKFSHVCTVPIKYDPAWA----TTSGDDNSPAAFIVTGAQLLVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           KQ DSK +LHLRLLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSL+ A+A+  KEK
Sbjct: 481 KQHDSKTVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAAL-KEK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP AA VIVDSGVFHGGPP+ +Q QKLVKFVELSEMR+GPQDSPG+W+VIGARLNLEKGK
Sbjct: 541 PPEAA-VIVDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I L VKFGLLHV+SET  D Q
Sbjct: 601 ICLQVKFGLLHVLSETITDDQ 613

BLAST of Tan0005303 vs. ExPASy TrEMBL
Match: A0A1S3CSS8 (LOW QUALITY PROTEIN: MACPF domain-containing protein At1g14780-like OS=Cucumis melo OX=3656 GN=LOC103504476 PE=4 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 546/621 (87.92%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 1   MGDGIVETALSSLGKGFDLTSDFRLKYCKGEQRLVLLNEAQRRELIVPGFGAVQDVSIDI 60
           M D IV+ AL+SLGKGFDLTSDFRLKYCKG   LVLLN++QRR+LIVPGFGAV+DVS+DI
Sbjct: 1   MADEIVQNALNSLGKGFDLTSDFRLKYCKGVHSLVLLNQSQRRDLIVPGFGAVKDVSVDI 60

Query: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCL 120
           KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFN+MFGFQSGCWA DAVNTKCL
Sbjct: 61  KCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNSMFGFQSGCWATDAVNTKCL 120

Query: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDV 180
           GLDGYFISLFNAHIDRYPLRLSDEVRSAVPS+WDPPAIARFIEKYGTHIIVGLSIGGHDV
Sbjct: 121 GLDGYFISLFNAHIDRYPLRLSDEVRSAVPSTWDPPAIARFIEKYGTHIIVGLSIGGHDV 180

Query: 181 VLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQL 240
           VLVRQDKSS LEPSELKNHLEELGDQLF GACTFPPPLSK KDHHK K PQAFNLFDPQL
Sbjct: 181 VLVRQDKSSKLEPSELKNHLEELGDQLFNGACTFPPPLSKPKDHHKPKIPQAFNLFDPQL 240

Query: 241 MAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFP 300
           +AFGNFSSVSAKDGIIVITSKRGGS TASSHCEWLPTV ESPDAIHFQFIPIISLLEG P
Sbjct: 241 VAFGNFSSVSAKDGIIVITSKRGGSPTASSHCEWLPTVHESPDAIHFQFIPIISLLEGIP 300

Query: 301 GKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLM 360
           GKGFLSHAINLYLRYKPPIADLQ+FLDFQVHKIWAPIHNDLPLGPTPNI+TSPSLQLNLM
Sbjct: 301 GKGFLSHAINLYLRYKPPIADLQYFLDFQVHKIWAPIHNDLPLGPTPNISTSPSLQLNLM 360

Query: 361 GPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPLWH 420
           GPKLYVNTTQV A+NKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQ+   WH
Sbjct: 361 GPKLYVNTTQVIADNKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQT--YWH 420

Query: 421 SSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHVI 480
           SSQHIA D Y+EPLNRNKFS++ T P+KYDP+WA    +TSGD NSPAAFIVTGAQL V+
Sbjct: 421 SSQHIADDRYVEPLNRNKFSHVCTVPIKYDPAWA----TTSGDDNSPAAFIVTGAQLLVV 480

Query: 481 KQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKEK 540
           KQ DSK +LHLRLLYSKVSNSY+VQTNWARHSSDISPKSGIFSAISTSL+ A+A+  KEK
Sbjct: 481 KQHDSKXVLHLRLLYSKVSNSYIVQTNWARHSSDISPKSGIFSAISTSLTGASAAL-KEK 540

Query: 541 PPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKGK 600
           PP AA VIVDSGVFHGGPP+ +Q QKLVKFVELSEMR+GPQDSPG+W+VIGARLNLEKGK
Sbjct: 541 PPEAA-VIVDSGVFHGGPPMSVQVQKLVKFVELSEMRKGPQDSPGYWMVIGARLNLEKGK 600

Query: 601 ISLHVKFGLLHVVSETSGDQQ 622
           I L VKFGLLHV+SET  D Q
Sbjct: 601 ICLQVKFGLLHVLSETITDDQ 613

BLAST of Tan0005303 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 687.6 bits (1773), Expect = 9.5e-198
Identity = 355/624 (56.89%), Postives = 448/624 (71.79%), Query Frame = 0

Query: 2   GDGIVETALSSLGKGFDLTSDFRLKYCK------GEQRLVLLNEAQRRELIVPGFGAVQD 61
           G  ++ETA+ SLGKGFDLT+DFRLKYCK      G+ RLV+L++ Q REL +PGFG  Q+
Sbjct: 5   GGDVIETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQN 64

Query: 62  VSIDIKCDRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAV 121
           VS DI CD+G+RTR++SDIL+FN+MSE FN++ S+ GKIPSG+FN  FGFQSG WA DA 
Sbjct: 65  VSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAA 124

Query: 122 NTKCLGLDGYFISLFNAHI-DRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLS 181
           N K LGLD   ++LFN HI +   LRL+D VR+AVPSSWDP  +ARFIE+YGTH+I G+S
Sbjct: 125 NVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVS 184

Query: 182 IGGHDVVLVRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHH---KTKTPQ 241
           +GG DVV+VRQDKSS L+   L++HL +LGDQLFTG+C           HH   + K P+
Sbjct: 185 VGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPE 244

Query: 242 AFNLF-DPQLMAFGNFSSVSAKDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFI 301
           AFN+F D Q +AF NF S+++++GI VI +KRGG   A SH EWL TV + PDAI+F FI
Sbjct: 245 AFNVFDDKQTVAFNNF-SINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 304

Query: 302 PIISLLEGFPGKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNIT 361
           PI SLL+  PG G LSHA++LYLRYKPP+ DLQ+FLDF   + WAP+HNDLP G  PN+ 
Sbjct: 305 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 364

Query: 362 TS-PSLQLNLMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLEN 421
           ++ P+L +N MGPKLYVNTT VT+E  PVTGMR +LEG K NRLAIH+QHL  T   +  
Sbjct: 365 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 424

Query: 422 NITQQSPPLWHSSQHIA-GDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPA 481
            IT +   +W  S  I   D Y EPLN  KFS++ T PVKYDP+W  ++S+    + +  
Sbjct: 425 KITDEH--IWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHK--SQNDV 484

Query: 482 AFIVTGAQLHVIKQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTS 541
           AFIVTGAQL V K+  SK++LHLRL Y+KVS+ YVVQ +W       S KSGIFS++S  
Sbjct: 485 AFIVTGAQLEV-KKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMP 544

Query: 542 L-SAATASTPKEKPPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHW 601
           L S +      +K  N   V++DSGVF GGPPVP    K+VKFV+LS++ RGPQ SPGHW
Sbjct: 545 LTSGSVHHNMIQKDKN--EVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHW 604

Query: 602 VVIGARLNLEKGKISLHVKFGLLH 612
           +V G RL L+KGK+ LHVKF LLH
Sbjct: 605 LVTGVRLYLDKGKLCLHVKFALLH 619

BLAST of Tan0005303 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 497.7 bits (1280), Expect = 1.4e-140
Identity = 278/615 (45.20%), Postives = 383/615 (62.28%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCKG---EQRLVLLNEAQRR-ELIVPGFGAVQDVSIDIKC 66
           E A+ S+G G+DL  D RLKYCKG   + RL+ + E     E+++PG  ++ +VS  IKC
Sbjct: 12  EVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKC 71

Query: 67  DRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCLGL 126
           D+G+R R++SDIL F QM+E FN++ S+ GKIPSG FN MF F S CW  DA  TK L  
Sbjct: 72  DKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYTKNLAF 131

Query: 127 DGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVL 186
           DG FISL++  +D+  + L + V+ AVPS+WDP A+ARFI+ YGTHIIV + +GG DV+ 
Sbjct: 132 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 191

Query: 187 VRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLS-KTKDHHKTKTPQAFNLFDPQLM 246
            +Q  SS L+P +L+  L+E+ D+ F  A       S + +   K +T +       Q +
Sbjct: 192 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE-------QRL 251

Query: 247 AFGNFSSVSA---KDGIIVITSKRGGSATAS-SHCEWLPTVLESPDAIHFQFIPIISLLE 306
            F + SS+ +   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL 
Sbjct: 252 RFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLN 311

Query: 307 GFPGKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQL 366
           G PG GFLSHAINLYLRYKPPI +L  FL+FQ+ + WAP+ ++LPLGP     +  SLQ 
Sbjct: 312 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQF 371

Query: 367 NLMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLL--LENNITQQS 426
           +  GPKLYVNTT V    +P+TGMRLYLEG +SNRLAIH+QHL + P +  LE+++ +  
Sbjct: 372 SFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI 431

Query: 427 PPLWHSSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGA 486
               H  +      Y E +N   +S++ T PV+ D   +                +VTGA
Sbjct: 432 RQESHDRR------YYEKVNWKNYSHVCTEPVESDDDLS----------------VVTGA 491

Query: 487 QLHVIKQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSA-ISTSLSAATA 546
           QLHV +    K +L LRL +S+V  + +V+ +    +   +PKSG+ S  IS   +AA  
Sbjct: 492 QLHV-ESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAA-- 551

Query: 547 STPKEKPPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARL 606
              ++ PP  A V ++S ++ GGPPVP QA KL+KFV+ SEM RGPQ+SPG+WVV GARL
Sbjct: 552 ---QKPPPRPADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARL 590

Query: 607 NLEKGKISLHVKFGL 610
            +EKGKISL VK+ L
Sbjct: 612 LVEKGKISLKVKYSL 590

BLAST of Tan0005303 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 475.3 bits (1222), Expect = 7.4e-134
Identity = 272/619 (43.94%), Postives = 373/619 (60.26%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCK---GEQRLVLLNEAQRRELIVPGFGAVQDVSIDIKCD 66
           E A+S +G G+DL SD R   CK      RLV ++  + R+LI PG   V +VS  IKCD
Sbjct: 16  EKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKCD 75

Query: 67  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCLGLD 126
           +G+RTR +SDIL+FNQMSE FN+   + GKIPSG FN MF F S CW  DA + K L  D
Sbjct: 76  KGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAF-SKCWPKDASSVKTLAYD 135

Query: 127 GYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 186
           G+FISL++  I R  L L DEV+  VPSSWD  A+A FIEKYGTH++VG+++GG DV+ V
Sbjct: 136 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 195

Query: 187 RQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQLMAF 246
           +Q + S+ EP E++  L+  GD+ F       P  SK+     +  P+  NL    L  F
Sbjct: 196 KQMRKSNHEPEEIQKMLKHWGDERF----CVDPVESKSPASVYSGKPKEENLLQWGLQPF 255

Query: 247 GNFSSVSA-----KDGIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEG 306
           G   S +       + I+ +  +RGG     SH  WL TV ++P+ I   F+PI SLL G
Sbjct: 256 GTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSG 315

Query: 307 FPGKGFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLN 366
            PG GFLSHA+NLYLRYKPPI +L  FL+FQ+ + WAP++ DLPLG   +  +SPSLQ +
Sbjct: 316 LPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFS 375

Query: 367 LMGPKLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSPPL 426
           LMGPKLYVNT++V +  +PVTG+R +LEG K N LAIH+QHL   P  L  +      P+
Sbjct: 376 LMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPI 435

Query: 427 WHSSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLH 486
               +     GY  P+    FS++ T PV+Y           +G  +   A IVT A L 
Sbjct: 436 ----EEPVEKGYYVPVKWGIFSHVCTYPVQY-----------NGARSDDTASIVTKAWLE 495

Query: 487 VIKQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPK 546
           V K    + +L LRL +S  +++   ++ W   S++ S KSG+FS IST LS   +  P 
Sbjct: 496 V-KGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTN-SRKSGVFSMISTRLSTGLSPNPA 555

Query: 547 EKPPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEK 606
              P  + + ++S V+  GP  P++  KL+  V+  E+ RGP++ PG+WVV GA+L +E 
Sbjct: 556 TTKPQ-SKIDINSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEA 610

Query: 607 GKISLHVKFGLLHVVSETS 618
           GKIS+  K+ LL V+SE S
Sbjct: 616 GKISIKAKYSLLTVISEDS 610

BLAST of Tan0005303 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 401.4 bits (1030), Expect = 1.4e-111
Identity = 240/612 (39.22%), Postives = 342/612 (55.88%), Query Frame = 0

Query: 6   VETALSSLGKGFDLTSDFRLKYCKGE--QRLVLLNEAQRRELIVPGFGAVQDVSIDIKCD 65
           +  A+ +LG+GFD+TSD RL YCKG    RLV + E Q R+L +     + +V  DI C 
Sbjct: 21  LRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCS 80

Query: 66  RGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCLGLD 125
           RG+    +  + +F++M+E FN +  + G IP G FN MF + +G W +DA +TK L L 
Sbjct: 81  RGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNY-TGSWQVDAASTKSLALV 140

Query: 126 GYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVLV 185
           GYFI L++  + +  L L +E+R AVPSSWDP ++A FIE YGTHI+  ++IGG DVV +
Sbjct: 141 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 200

Query: 186 RQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLSKTKDHHKTKTPQAFNLFDPQLMAF 245
           RQ +SS L  SE++N++ ++                     H+    ++ ++  P     
Sbjct: 201 RQHQSSPLPVSEIENYVNDM-------------------IKHRFHEAESQSITGP----- 260

Query: 246 GNFSSVSAKD-GIIVITSKRGGSATASSHCEWLPTVLESPDAIHFQFIPIISLLEGFPGK 305
                +  KD  I VI  +RGG     SH  W  TV  +PD I+  F PI+SLLEG PG 
Sbjct: 261 -----LKYKDKDITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGL 320

Query: 306 GFLSHAINLYLRYKPPIADLQHFLDFQVHKIWAPIHNDLPLGPTPNITTSPSLQLNLMGP 365
             L+ AI LYL YKPPI DLQ+FLD+Q+ + WAP  ++L            SLQ +LMGP
Sbjct: 321 RHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGP 380

Query: 366 KLYVNTTQVTAENKPVTGMRLYLEGIKSNRLAIHIQHLLTTPLLLENNITQQSP---PLW 425
           KL+++  QVT   KPVTG+RL LEG K NRL+IH+QHL++ P +L+ +     P   P W
Sbjct: 381 KLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKW 440

Query: 426 HSSQHIAGDGYIEPLNRNKFSYISTAPVKYDPSWAASSSSTSGDANSPAAFIVTGAQLHV 485
              +      + EP+    FS++ST+P+++       + +  GD +     IVTGAQL V
Sbjct: 441 QGPEE-QDSRWFEPIKWKNFSHVSTSPIEH-------TETHIGDLS--GVHIVTGAQLGV 500

Query: 486 IKQQDSKAILHLRLLYSKVSNSYVVQTNWARHSSDISPKSGIFSAISTSLSAATASTPKE 545
                SK +LHL+LL+SKV    + ++ W     D +P                AS+ + 
Sbjct: 501 -WDFGSKNVLHLKLLFSKVPGCTIRRSVW-----DHTP---------------VASSGRL 560

Query: 546 KPPNAATVIVDSGVFHGGPPVPLQAQKLVKFVELSEMRRGPQDSPGHWVVIGARLNLEKG 605
           +P   +T             V  Q+ KL K V+ SEM +GPQD PGHW+V GA+L +EKG
Sbjct: 561 EPGGPST-------SSSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKG 560

Query: 606 KISLHVKFGLLH 612
           KI L VK+ LL+
Sbjct: 621 KIVLRVKYSLLN 560

BLAST of Tan0005303 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 274.6 bits (701), Expect = 1.9e-73
Identity = 147/320 (45.94%), Postives = 201/320 (62.81%), Query Frame = 0

Query: 7   ETALSSLGKGFDLTSDFRLKYCKG---EQRLVLLNEAQRR-ELIVPGFGAVQDVSIDIKC 66
           E A+ S+G G+DL  D RLKYCKG   + RL+ + E     E+++PG  ++ +VS  IKC
Sbjct: 12  EVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKC 71

Query: 67  DRGDRTRYQSDILNFNQMSELFNRKCSIPGKIPSGSFNTMFGFQSGCWAMDAVNTKCLGL 126
           D+G+R R++SDIL F QM+E FN++ S+ GKIPSG FN MF F S CW  DA  TK L  
Sbjct: 72  DKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEF-SSCWQKDAAYTKNLAF 131

Query: 127 DGYFISLFNAHIDRYPLRLSDEVRSAVPSSWDPPAIARFIEKYGTHIIVGLSIGGHDVVL 186
           DG FISL++  +D+  + L + V+ AVPS+WDP A+ARFI+ YGTHIIV + +GG DV+ 
Sbjct: 132 DGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIY 191

Query: 187 VRQDKSSSLEPSELKNHLEELGDQLFTGACTFPPPLS-KTKDHHKTKTPQAFNLFDPQLM 246
            +Q  SS L+P +L+  L+E+ D+ F  A       S + +   K +T +       Q +
Sbjct: 192 AKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE-------QRL 251

Query: 247 AFGNFSSVSA---KDGIIVITSKRGGSATAS-SHCEWLPTVLESPDAIHFQFIPIISLLE 306
            F + SS+ +   K+  + +  +RGG+   +  H EWL TV   PD I   FIPI SLL 
Sbjct: 252 RFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLN 311

Query: 307 GFPGKGFLSHAINLYLRYKP 318
           G PG GFLSHAINLYLR KP
Sbjct: 312 GVPGSGFLSHAINLYLRCKP 323