Tan0004831 (gene) Snake gourd v1

Overview


Name	Tan0004831
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	nicotianamine synthase-like
Location	LG08: 6591165 .. 6592050 (+)
RNA-Seq Expression	Tan0004831
Synteny	Tan0004831

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTTCAAAGCTTTTGAACACAAAACTCAGAACTTCACCATGTGTTGCCAAGGTCTCCTTTTGGTCCAAAAAATCTCAGAACTTTACCACAAAATCTCCACTCTCGAAAGCCTCAAACCCTCCAAAGATGTCAACTCCCTCTTCTCTCAACTCGTCCTCACTTGCACCCCTCCTCCGCCGCCGTCTCCGCCGCCGCCCATCGACGTCTCCAAGCTTTGCTCCGCCGTCCAAAACATGAGATCCCATCTCATCAAACTCTGCGGCGAAGCTGAGTCCCTTCTCGAACACCATTACTCCACCATTTTAGCCTCCCATCAAAACCCACTTCACCATCTCTCCATTTTCCCTTATTACACCAACTACCTCAAACTTACCCACCTCGAATTCTCCATTTTGACCCCCCACGCGCCCCACGCGCCGCCCTCCAAGGTGGCATTTATCGGCTCCGGCGCGCTTCCCTTCTCGTCCATCATCATGGCGTCAAAATACCTCCCGACGACCGAGTTTCACAACTTCGACATCGACCGTCGCGCCAACGCAAAGCGCCAGCGCGACTCGCAAGTGGCAAGCGACGCGGACTTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGCCACCTCGAACGAGTTGGGAGAGTTCGAAGTGGTGTTTTTGGCGGCGTTGGTGGGGATGGAGAGGGAAGAGAAGGTGAAGGTGATCGAGCATTTAGCGAAGAACATGGCGGGTGGCGCGTTGTTGATGTTGAGGAGCGCGTATGGTGCACGCGCGTTTCTTTATCCGGTGGTGGAAGCTTGTGATCTTCGAGGGTTTGAGATTCTTACAGTGTTTCATCCTACTGATGAGGTTATCAATTCAATCGTGTTGGCAAGATTATAA

mRNA sequence

ATTTTTCAAAGCTTTTGAACACAAAACTCAGAACTTCACCATGTGTTGCCAAGGTCTCCTTTTGGTCCAAAAAATCTCAGAACTTTACCACAAAATCTCCACTCTCGAAAGCCTCAAACCCTCCAAAGATGTCAACTCCCTCTTCTCTCAACTCGTCCTCACTTGCACCCCTCCTCCGCCGCCGTCTCCGCCGCCGCCCATCGACGTCTCCAAGCTTTGCTCCGCCGTCCAAAACATGAGATCCCATCTCATCAAACTCTGCGGCGAAGCTGAGTCCCTTCTCGAACACCATTACTCCACCATTTTAGCCTCCCATCAAAACCCACTTCACCATCTCTCCATTTTCCCTTATTACACCAACTACCTCAAACTTACCCACCTCGAATTCTCCATTTTGACCCCCCACGCGCCCCACGCGCCGCCCTCCAAGGTGGCATTTATCGGCTCCGGCGCGCTTCCCTTCTCGTCCATCATCATGGCGTCAAAATACCTCCCGACGACCGAGTTTCACAACTTCGACATCGACCGTCGCGCCAACGCAAAGCGCCAGCGCGACTCGCAAGTGGCAAGCGACGCGGACTTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGCCACCTCGAACGAGTTGGGAGAGTTCGAAGTGGTGTTTTTGGCGGCGTTGGTGGGGATGGAGAGGGAAGAGAAGGTGAAGGTGATCGAGCATTTAGCGAAGAACATGGCGGGTGGCGCGTTGTTGATGTTGAGGAGCGCGTATGGTGCACGCGCGTTTCTTTATCCGGTGGTGGAAGCTTGTGATCTTCGAGGGTTTGAGATTCTTACAGTGTTTCATCCTACTGATGAGGTTATCAATTCAATCGTGTTGGCAAGATTATAA

Coding sequence (CDS)

ATGTGTTGCCAAGGTCTCCTTTTGGTCCAAAAAATCTCAGAACTTTACCACAAAATCTCCACTCTCGAAAGCCTCAAACCCTCCAAAGATGTCAACTCCCTCTTCTCTCAACTCGTCCTCACTTGCACCCCTCCTCCGCCGCCGTCTCCGCCGCCGCCCATCGACGTCTCCAAGCTTTGCTCCGCCGTCCAAAACATGAGATCCCATCTCATCAAACTCTGCGGCGAAGCTGAGTCCCTTCTCGAACACCATTACTCCACCATTTTAGCCTCCCATCAAAACCCACTTCACCATCTCTCCATTTTCCCTTATTACACCAACTACCTCAAACTTACCCACCTCGAATTCTCCATTTTGACCCCCCACGCGCCCCACGCGCCGCCCTCCAAGGTGGCATTTATCGGCTCCGGCGCGCTTCCCTTCTCGTCCATCATCATGGCGTCAAAATACCTCCCGACGACCGAGTTTCACAACTTCGACATCGACCGTCGCGCCAACGCAAAGCGCCAGCGCGACTCGCAAGTGGCAAGCGACGCGGACTTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGCCACCTCGAACGAGTTGGGAGAGTTCGAAGTGGTGTTTTTGGCGGCGTTGGTGGGGATGGAGAGGGAAGAGAAGGTGAAGGTGATCGAGCATTTAGCGAAGAACATGGCGGGTGGCGCGTTGTTGATGTTGAGGAGCGCGTATGGTGCACGCGCGTTTCTTTATCCGGTGGTGGAAGCTTGTGATCTTCGAGGGTTTGAGATTCTTACAGTGTTTCATCCTACTGATGAGGTTATCAATTCAATCGTGTTGGCAAGATTATAA

Protein sequence

MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLCSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFSILTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASDADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL

Homology

[Close]

BLAST of Tan0004831 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 5.6e-86
Identity = 171/279 (61.29%), Postives = 212/279 (75.99%), Query Frame = 0


Query: 3   CQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLCSA 62
           CQ   LV KI +LY KIS LE+LKP +DV++LF QLV TC P     P P IDV+K+   
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIP-----PNPNIDVTKMSEN 63

Query: 63  VQNMRSHLIKLCGEAESLLEHHYSTILASHQ-NPLHHLSIFPYYTNYLKLTHLEFSILTP 122
           +Q MRS+LIK+CGEAE  LEHH+S+IL S + NPLHHL++FPYY NYLKL+ LEF +L  
Sbjct: 64  IQEMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQ 123

Query: 123 HAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASDADL 182
           +     P  VAFIGSG LP +S+++AS +L  + FHNFDID  AN    R   V+SD DL
Sbjct: 124 NLNGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAAR--LVSSDPDL 183

Query: 183 SRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLMLRSA 242
           S+RM FHT DIMD T +  G F+VVFLAALVGM+++EKVKV+EHL K+M+ GALLMLRSA
Sbjct: 184 SQRMFFHTVDIMDVTESLKG-FDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSA 243

Query: 243 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 281
           +G RAFLYP+VE CDL GFE+L+V+HPTDEVINSIV++R
Sbjct: 244 HGPRAFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of Tan0004831 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 9.6e-86
Identity = 166/280 (59.29%), Postives = 217/280 (77.50%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLC 60
           +C     +V+K+ ELY +IS LE+L PSKDVN LF+ LV TC       PP PIDVSKLC
Sbjct: 2   VCPNSNPVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTC------MPPNPIDVSKLC 61

Query: 61  SAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFSILT 120
             +Q +RSHLIKLCG+AE LLE H+S IL+S++NPL HL IFPY+ NY+KL+ LE++ILT
Sbjct: 62  QKIQEIRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILT 121

Query: 121 PHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASDAD 180
            +  +  P K+AFIGSG LP +S+++A+K+L TT FHN+DID  AN      + VA+D D
Sbjct: 122 KNTTNI-PKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANF--MASALVAADPD 181

Query: 181 LSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLMLRS 240
           +S RM FHT D+MD T   L +++VVFLAALVGM++E+KVKV++HLAK M+ GA LMLRS
Sbjct: 182 MSSRMTFHTADVMDVTC-ALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRS 241

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 281
           A+GARAFLYPV++  DLRGFE+L+V+HPTDEVINS+++AR
Sbjct: 242 AHGARAFLYPVLDPRDLRGFEVLSVYHPTDEVINSVIIAR 271

[Close]

BLAST of Tan0004831 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.6e-85
Identity = 173/281 (61.57%), Postives = 212/281 (75.44%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLC 60
           M CQ   LVQ I +LY KIS LESLKPS+DVN LF QLV TC P     P P IDV+K+C
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIP-----PNPNIDVTKMC 60

Query: 61  SAVQNMRSHLIKLCGEAESLLEHHYSTILASHQ-NPLHHLSIFPYYTNYLKLTHLEFSIL 120
             VQ +R +LIK+CG AE  LE+H+S+IL S+Q NPLHHL+IFPYY NYLKL  LEF +L
Sbjct: 61  DRVQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLL 120

Query: 121 TPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASDA 180
             +     P  VAFIGSG LP +SI++AS +L  T FHNFDID  AN+       V+SD 
Sbjct: 121 EQNLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANS--LASLLVSSDP 180

Query: 181 DLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLMLR 240
           D+S+RM FHT DIMD T + L  F+VVFLAALVGM +EEKVKVIEHL K+MA GA+LMLR
Sbjct: 181 DISQRMFFHTVDIMDVTES-LKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 281
           SA+G RAFLYP+VE CDL+GFE+L+++HPTD+VINS+V+++
Sbjct: 241 SAHGPRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of Tan0004831 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 6.9e-84
Identity = 162/283 (57.24%), Postives = 217/283 (76.68%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLC 60
           M CQ  L+V++I +LY +IS L+SLKPSK+V++LF QLV TC P         IDV+ +C
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLP-----TDTNIDVTNMC 60

Query: 61  SAVQNMRSHLIKLCGEAESLLEHHYSTILAS---HQNPLHHLSIFPYYTNYLKLTHLEFS 120
             V++MR++LIKLCGEAE  LE H+STIL S    QNPL HL IFPYY+NYLKL  LEF 
Sbjct: 61  EEVKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFD 120

Query: 121 ILTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVAS 180
           +L+ H+ H  P+K+AF+GSG +P +SI++A  +LP T FHNFDID  AN      + V+ 
Sbjct: 121 LLSQHSSHV-PTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANT--LASNLVSR 180

Query: 181 DADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLM 240
           D DLS+RM+FHTTD+++AT   L +++VVFLAALVGM++E KVK IEHL K+MA GA+LM
Sbjct: 181 DPDLSKRMIFHTTDVLNATEG-LDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLM 240

Query: 241 LRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 281
           LRSA+  RAFLYP+V++ DL+GF++LT++HPTD+V+NS+V+AR
Sbjct: 241 LRSAHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of Tan0004831 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.0e-83
Identity = 163/282 (57.80%), Postives = 218/282 (77.30%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKL- 60
           M C+  L+V++I +LY++IS LESLKPSK+V++LF QLV TC P         IDV+++ 
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLP-----TDTNIDVTEIH 60

Query: 61  CSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQ-NPLHHLSIFPYYTNYLKLTHLEFSI 120
              V++MRSHLIKLCGEAE  LE H+S IL S + NPL+HL IFPYY NYLKL  LEF +
Sbjct: 61  DEKVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDL 120

Query: 121 LTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASD 180
           L+ H  H  P+KVAFIGSG +P +SI++A  +LP T FHNFDID  AN      + V+ D
Sbjct: 121 LSQHTTHV-PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANT--LASNLVSRD 180

Query: 181 ADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLML 240
           +DLS+RM+FHTTD+++A    L +++VVFLAALVGM++E KVK IEHL K+MA GA++ML
Sbjct: 181 SDLSKRMIFHTTDVLNAKEG-LDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVML 240

Query: 241 RSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 281
           RSA+G RAFLYP+V++CDL+GFE+LT++HP+D+V+NS+V+AR
Sbjct: 241 RSAHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of Tan0004831 vs. NCBI nr
Match: XP_038891237.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 461.8 bits (1187), Expect = 4.1e-126
Identity = 243/281 (86.48%), Postives = 253/281 (90.04%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLC 60
           MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLF+QLVL C+  PPPS P  IDVS LC
Sbjct: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFTQLVLICS--PPPSSPSSIDVSTLC 60

Query: 61  SAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFSILT 120
           SAVQ MRSHLIKLCGEAE+LLEHHYSTILASHQNPL HLS+FPYY+NYLKLTHLEFSIL 
Sbjct: 61  SAVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILN 120

Query: 121 PHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASDAD 180
            HAPHAPPSKVAF+GSGALPFSSIIMA KYLP TE HNFDID  ANAK      VA DA+
Sbjct: 121 HHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKTELHNFDIDPSANAKAA--MLVAGDAE 180

Query: 181 LSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLMLRS 240
           LS+RMVFHTTDIMD  S+EL EFEVVFLAALVGMEREEKVKVIEHL K+M GGALLMLRS
Sbjct: 181 LSKRMVFHTTDIMDVASDELREFEVVFLAALVGMEREEKVKVIEHLRKSMGGGALLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 277

[Close]

BLAST of Tan0004831 vs. NCBI nr
Match: KAG7021049.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 449.5 bits (1155), Expect = 2.1e-122
Identity = 235/284 (82.75%), Postives = 253/284 (89.08%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPP-IDVS 60
           MCC  +GLLLVQK+SELYHKISTL+SL+PSK VNSLFSQLVLTCTPPPPP P PP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPL HLS+FPYY+NYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVAS 180
           ILT +   +PPSKVAFIGSGALPFSSIIMA KYLPTTEFHNFD+D  ANAK    + VAS
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAK--ATTLVAS 180

Query: 181 DADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLM 240
           D DLS+RM+FHTTDIMD TSN LGEFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLM
Sbjct: 181 DVDLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLM 240

Query: 241 LRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           LRSAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Tan0004831 vs. NCBI nr
Match: KAG6586217.1 (putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 446.4 bits (1147), Expect = 1.8e-121
Identity = 233/284 (82.04%), Postives = 253/284 (89.08%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPP-IDVS 60
           MCC  +GLLLVQK+SELYHKISTL++L+PSK VNSLFSQLVLTCTPPPPP P PP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQTLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPL HLS+FPYY+NYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVAS 180
           ILT +   +PPSKVAFIGSGALPFSSIIMA KYLPTTEFHNFD+D  ANAK    + VAS
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAK--ATTLVAS 180

Query: 181 DADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLM 240
           D DLS+RM+FHTTDIMD TS+ LGEFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLM
Sbjct: 181 DVDLSKRMLFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLQKNMTSGSLLM 240

Query: 241 LRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           LRSAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Tan0004831 vs. NCBI nr
Match: XP_023538584.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 445.3 bits (1144), Expect = 4.0e-121
Identity = 234/284 (82.39%), Postives = 251/284 (88.38%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPP-IDVS 60
           MCC  +GLLLVQK+SELYHKISTL+SL+PSK VNSLFSQLVLTCTPPPPP P PP IDVS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDVS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFS 120
            L S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPL HLS+FPYY+NYLKLTHLEFS
Sbjct: 61  NLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVAS 180
           ILT +   +PPSKVAFIGSGALPFSSIIMA KYLPTTEFHNFD+D  ANAK    + VAS
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAM--ALVAS 180

Query: 181 DADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLM 240
           D DLS+RM+FHTTDIMD TSN L EFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLM
Sbjct: 181 DVDLSKRMLFHTTDIMDVTSNMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLM 240

Query: 241 LRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           LRSAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Tan0004831 vs. NCBI nr
Match: XP_022938003.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-120
Identity = 234/284 (82.39%), Postives = 251/284 (88.38%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPP-IDVS 60
           MCC  +GLLLVQK+SELYHKISTL+SL+PSK VNSLFSQLVLTCTPPPPP P PP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPL HLS+FPYY+NYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVAS 180
           ILT +   + PSKVAFIGSGALPFSSIIMA KYLPTTEFHNFD+D  ANAK    + VAS
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAK--ATALVAS 180

Query: 181 DADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLM 240
           D DLSRRM FHTTDIMD TS+ LGEFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLM
Sbjct: 181 DVDLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLM 240

Query: 241 LRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           LRSAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Tan0004831 vs. ExPASy TrEMBL
Match: A0A6J1FHL8 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 5.6e-121
Identity = 234/284 (82.39%), Postives = 251/284 (88.38%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPP-IDVS 60
           MCC  +GLLLVQK+SELYHKISTL+SL+PSK VNSLFSQLVLTCTPPPPP P PP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPL HLS+FPYY+NYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVAS 180
           ILT +   + PSKVAFIGSGALPFSSIIMA KYLPTTEFHNFD+D  ANAK    + VAS
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAK--ATALVAS 180

Query: 181 DADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLM 240
           D DLSRRM FHTTDIMD TS+ LGEFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLM
Sbjct: 181 DVDLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLM 240

Query: 241 LRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           LRSAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Tan0004831 vs. ExPASy TrEMBL
Match: A0A6J1HS54 (nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.8e-120
Identity = 234/284 (82.39%), Postives = 249/284 (87.68%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPP-IDVS 60
           MCC  +GLLLVQK+SELYHKISTL+SL+PSK VNSLFSQLVLTCTPPPPP P PP ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLCSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYSTILASHQNPL HLS+FPYY+NYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVAS 180
           ILT +   A PSKVAFIGSGALPFSSIIMA KYLPTTEFHNFD+D  ANAK      VAS
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAK--ATMLVAS 180

Query: 181 DADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLM 240
           D DLSRRM FHTTDIMD TS+ L EFEVVFLAALVGME+EEKVKVIEHL KNM  G+LLM
Sbjct: 181 DVDLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLM 240

Query: 241 LRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           LRSAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Tan0004831 vs. ExPASy TrEMBL
Match: A0A1S3BXJ6 (nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 2.6e-118
Identity = 234/282 (82.98%), Postives = 244/282 (86.52%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLV-LTCTPPPPPSPPPPIDVSKL 60
           MCCQ LLLVQKISELYHKISTLESLKPSK VNSLFSQLV L CTPP   S P  IDVS L
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60

Query: 61  CSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFSIL 120
           CS++Q MRS+LIKLCGEAESLLEHHYSTIL+SHQNPL HLS+FPYY+NYLKLTHLEFSIL
Sbjct: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 TPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASDA 180
             H    PPSKVAF+GSGALPFSSIIMA KYLP TEFHNFD+D  ANAK      VA D 
Sbjct: 121 NHHIT-PPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAK--ASMLVAGDV 180

Query: 181 DLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLMLR 240
           +LSRRMVFHTTDIM+  S EL EFEVVFLAALVGMEREEK KVIEHL KNM GGALLMLR
Sbjct: 181 ELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

[Close]

BLAST of Tan0004831 vs. ExPASy TrEMBL
Match: A0A0A0LJ09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.3e-117
Identity = 232/284 (81.69%), Postives = 243/284 (85.56%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLC 60
           MCCQ LLLVQKISELYHKISTLESLKPSK VNSLFSQLVL    P   S    IDVS LC
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPSSSS----IDVSTLC 60

Query: 61  SAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFSILT 120
           S +Q MRSHLIKLCGEAESLLEHHYSTIL+SHQNPL HLS+FPYY+NYLKLTHLEFSIL 
Sbjct: 61  STIQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILN 120

Query: 121 PHAPHA---PPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVAS 180
            HAPH+   PPSKVAF+GSGALPFSSIIMA KY P TEFHNFD+D  ANAK      VA 
Sbjct: 121 HHAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAK--ASMLVAG 180

Query: 181 DADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLM 240
           D +LSRRMVFHTTDIMD  S EL EFEVVFLAALVGMEREEK KVIEHL KNM+GGALLM
Sbjct: 181 DVELSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLM 240

Query: 241 LRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           LRSAYGARAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 241 LRSAYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of Tan0004831 vs. ExPASy TrEMBL
Match: A0A6J1DTD8 (probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.9e-116
Identity = 230/281 (81.85%), Postives = 246/281 (87.54%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLC 60
           MCCQ +LLVQK+SELYHKISTL+SLKPSKDVNSLFSQLVLTC PPPP   PPPIDVSKL 
Sbjct: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPP--SPPPIDVSKLG 60

Query: 61  SAVQNMRSHLIKLCGEAESLLEHHYSTILASHQNPLHHLSIFPYYTNYLKLTHLEFSILT 120
           SAVQ MRSHLIKLCGEAE+LLEHHYS+ILASH+NPL +LSIFPYY+NYLKL HLEFSIL 
Sbjct: 61  SAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSIL- 120

Query: 121 PHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASDAD 180
               HA PSKVAF+GSGALPF+SIIMASKYLP TEFHNFD+D  ANAK    + VA D D
Sbjct: 121 --ERHAQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAA--ALVAGDRD 180

Query: 181 LSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLMLRS 240
           L RRMVFHT DIM+ T  EL  F+VVFLAALVGMEREEKVKVIEHL KNM GGALLMLRS
Sbjct: 181 LERRMVFHTADIMEVT-GELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 282
           AYGARAFLYPVVEA DL+GFEILTVFHPTDEVINS+VLARL
Sbjct: 241 AYGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273

[Close]

BLAST of Tan0004831 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 318.9 bits (816), Expect = 4.0e-87
Identity = 171/279 (61.29%), Postives = 212/279 (75.99%), Query Frame = 0


Query: 3   CQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLCSA 62
           CQ   LV KI +LY KIS LE+LKP +DV++LF QLV TC P     P P IDV+K+   
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIP-----PNPNIDVTKMSEN 63

Query: 63  VQNMRSHLIKLCGEAESLLEHHYSTILASHQ-NPLHHLSIFPYYTNYLKLTHLEFSILTP 122
           +Q MRS+LIK+CGEAE  LEHH+S+IL S + NPLHHL++FPYY NYLKL+ LEF +L  
Sbjct: 64  IQEMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQ 123

Query: 123 HAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASDADL 182
           +     P  VAFIGSG LP +S+++AS +L  + FHNFDID  AN    R   V+SD DL
Sbjct: 124 NLNGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAAR--LVSSDPDL 183

Query: 183 SRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLMLRSA 242
           S+RM FHT DIMD T +  G F+VVFLAALVGM+++EKVKV+EHL K+M+ GALLMLRSA
Sbjct: 184 SQRMFFHTVDIMDVTESLKG-FDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSA 243

Query: 243 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 281
           +G RAFLYP+VE CDL GFE+L+V+HPTDEVINSIV++R
Sbjct: 244 HGPRAFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of Tan0004831 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 317.4 bits (812), Expect = 1.2e-86
Identity = 173/281 (61.57%), Postives = 212/281 (75.44%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLC 60
           M CQ   LVQ I +LY KIS LESLKPS+DVN LF QLV TC P     P P IDV+K+C
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIP-----PNPNIDVTKMC 60

Query: 61  SAVQNMRSHLIKLCGEAESLLEHHYSTILASHQ-NPLHHLSIFPYYTNYLKLTHLEFSIL 120
             VQ +R +LIK+CG AE  LE+H+S+IL S+Q NPLHHL+IFPYY NYLKL  LEF +L
Sbjct: 61  DRVQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLL 120

Query: 121 TPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASDA 180
             +     P  VAFIGSG LP +SI++AS +L  T FHNFDID  AN+       V+SD 
Sbjct: 121 EQNLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANS--LASLLVSSDP 180

Query: 181 DLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLMLR 240
           D+S+RM FHT DIMD T + L  F+VVFLAALVGM +EEKVKVIEHL K+MA GA+LMLR
Sbjct: 181 DISQRMFFHTVDIMDVTES-LKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 281
           SA+G RAFLYP+VE CDL+GFE+L+++HPTD+VINS+V+++
Sbjct: 241 SAHGPRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of Tan0004831 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 312.0 bits (798), Expect = 4.9e-85
Identity = 162/283 (57.24%), Postives = 217/283 (76.68%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKLC 60
           M CQ  L+V++I +LY +IS L+SLKPSK+V++LF QLV TC P         IDV+ +C
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLP-----TDTNIDVTNMC 60

Query: 61  SAVQNMRSHLIKLCGEAESLLEHHYSTILAS---HQNPLHHLSIFPYYTNYLKLTHLEFS 120
             V++MR++LIKLCGEAE  LE H+STIL S    QNPL HL IFPYY+NYLKL  LEF 
Sbjct: 61  EEVKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFD 120

Query: 121 ILTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVAS 180
           +L+ H+ H  P+K+AF+GSG +P +SI++A  +LP T FHNFDID  AN      + V+ 
Sbjct: 121 LLSQHSSHV-PTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANT--LASNLVSR 180

Query: 181 DADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLM 240
           D DLS+RM+FHTTD+++AT   L +++VVFLAALVGM++E KVK IEHL K+MA GA+LM
Sbjct: 181 DPDLSKRMIFHTTDVLNATEG-LDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLM 240

Query: 241 LRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 281
           LRSA+  RAFLYP+V++ DL+GF++LT++HPTD+V+NS+V+AR
Sbjct: 241 LRSAHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of Tan0004831 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 310.5 bits (794), Expect = 1.4e-84
Identity = 163/282 (57.80%), Postives = 218/282 (77.30%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLTCTPPPPPSPPPPIDVSKL- 60
           M C+  L+V++I +LY++IS LESLKPSK+V++LF QLV TC P         IDV+++ 
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLP-----TDTNIDVTEIH 60

Query: 61  CSAVQNMRSHLIKLCGEAESLLEHHYSTILASHQ-NPLHHLSIFPYYTNYLKLTHLEFSI 120
              V++MRSHLIKLCGEAE  LE H+S IL S + NPL+HL IFPYY NYLKL  LEF +
Sbjct: 61  DEKVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDL 120

Query: 121 LTPHAPHAPPSKVAFIGSGALPFSSIIMASKYLPTTEFHNFDIDRRANAKRQRDSQVASD 180
           L+ H  H  P+KVAFIGSG +P +SI++A  +LP T FHNFDID  AN      + V+ D
Sbjct: 121 LSQHTTHV-PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANT--LASNLVSRD 180

Query: 181 ADLSRRMVFHTTDIMDATSNELGEFEVVFLAALVGMEREEKVKVIEHLAKNMAGGALLML 240
           +DLS+RM+FHTTD+++A    L +++VVFLAALVGM++E KVK IEHL K+MA GA++ML
Sbjct: 181 SDLSKRMIFHTTDVLNAKEG-LDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVML 240

Query: 241 RSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 281
           RSA+G RAFLYP+V++CDL+GFE+LT++HP+D+V+NS+V+AR
Sbjct: 241 RSAHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of Tan0004831 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 62.4 bits (150), Expect = 6.7e-10
Identity = 30/53 (56.60%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 230 MAGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHP--TDEVINSIVLAR 281
           M  GALLMLRSA G R+FLY  V+ CDL+GFE+L ++HP  +D  +NS+++AR
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVAR 53

The following BLAST results are available for this feature:

BLAST of Tan0004831 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	5.6e-86	61.29	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9XGI7	9.6e-86	59.29	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
O80483	1.6e-85	61.57	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	6.9e-84	57.24	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	2.0e-83	57.80	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Tan0004831 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891237.1	4.1e-126	86.48	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
KAG7021049.1	2.1e-122	82.75	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6586217.1	1.8e-121	82.04	putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023538584.1	4.0e-121	82.39	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022938003.1	1.2e-120	82.39	nicotianamine synthase-like [Cucurbita moschata]	[more]

BLAST of Tan0004831 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FHL8	5.6e-121	82.39	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 S...	[more]
A0A6J1HS54	2.8e-120	82.39	nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1	[more]
A0A1S3BXJ6	2.6e-118	82.98	nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1	[more]
A0A0A0LJ09	1.3e-117	81.69	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1	[more]
A0A6J1DTD8	1.9e-116	81.85	probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309...	[more]

BLAST of Tan0004831 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	4.0e-87	61.29	nicotianamine synthase 4	[more]
AT1G09240.1	1.2e-86	61.57	nicotianamine synthase 3	[more]
AT5G04950.1	4.9e-85	57.24	nicotianamine synthase 1	[more]
AT5G56080.1	1.4e-84	57.80	nicotianamine synthase 2	[more]
AT4G26483.1	6.7e-10	56.60	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 6..281 e-value: 7.9E-58 score: 197.7
None	No IPR available	PANTHER	PTHR32266:SF17	SYNTHASE, PUTATIVE-RELATED	coord: 1..280
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 7..280 e-value: 1.6E-111 score: 372.1
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..280
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..281 score: 53.203941

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0004831.1	Tan0004831.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity