Tan0004757 (gene) Snake gourd v1

Overview


Name	Tan0004757
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	rRNA N-glycosidase
Location	LG06: 30584735 .. 30585631 (+)
RNA-Seq Expression	Tan0004757
Synteny	Tan0004757

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAAATTCATAGCACTCTCCTTTTTCTTTCTCGCCATCTCTCTTGGCTCTCCTACTGCCATTGGTGATGTTAGCTTCGATTTGTCGACAGCTACTAAAAAATCCTATTCATCTTTCATCACACAACTCAGGGATGCTCTTCCAACTCAAGGCACAGTGTGCGGCATTCCATTGCTGCCTTCCACCGCATCCGGCTCACAATGGTTCAGATTCTTCAATCTTACCAATTATAACGATGAAACCGTCACCGTGGCTGTAAATGTAACCAATGTCTACATCGTGGCGTATCGTGCCGATGCTGTATCCTACTTTTTTGAAGACACTCCAGCTGAAGCTTTCAAGCTCATATTTGCAGGTACTAAGACGGTAAAACTTCCTTATTCGGGTAATTATGATAAGCTTCAAAGTGTAGTAGGCAAACAAAGAGATATGATTGAGCTTGGAATCCCGGCTTTAAGCAGTGCCATTACAAACATGGTTTATTACGACTACCAAAGTACTGCAGCCGCGCTTCTTGTACTCATTCAGTGTACTGCAGAAGCTGCAAGGTATAAATATATTGAGCAACAAGTTTCTTCACATATTAGCTCTAATTTTTATCCAAATCAAGCAGTCATAAGCTTAGAAAACAAGTGGGGTGCTCTCTCCAAACAAATCCAGATAGCAAATAGGACGGGACATGGACAATTCGAAAACCCTGTTGAGCTATATAACCCTGATGGCACACGATTTAGTGTAACCAATACTTCGGCTGGAGTTGTAAAAGGCAATATCAAACTCCTACTATACTACAAAGCCAGTGTTGGTAGTGAATATGATATCCCTACTACAATCTTGCACCCTGGAGCTATGGGAATGCTTCACAATCAGAATGGAAATTATGTTACAATGTGA

mRNA sequence

ATGAACAAATTCATAGCACTCTCCTTTTTCTTTCTCGCCATCTCTCTTGGCTCTCCTACTGCCATTGGTGATGTTAGCTTCGATTTGTCGACAGCTACTAAAAAATCCTATTCATCTTTCATCACACAACTCAGGGATGCTCTTCCAACTCAAGGCACAGTGTGCGGCATTCCATTGCTGCCTTCCACCGCATCCGGCTCACAATGGTTCAGATTCTTCAATCTTACCAATTATAACGATGAAACCGTCACCGTGGCTGTAAATGTAACCAATGTCTACATCGTGGCGTATCGTGCCGATGCTGTATCCTACTTTTTTGAAGACACTCCAGCTGAAGCTTTCAAGCTCATATTTGCAGGTACTAAGACGGTAAAACTTCCTTATTCGGGTAATTATGATAAGCTTCAAAGTGTAGTAGGCAAACAAAGAGATATGATTGAGCTTGGAATCCCGGCTTTAAGCAGTGCCATTACAAACATGGTTTATTACGACTACCAAAGTACTGCAGCCGCGCTTCTTGTACTCATTCAGTGTACTGCAGAAGCTGCAAGGTATAAATATATTGAGCAACAAGTTTCTTCACATATTAGCTCTAATTTTTATCCAAATCAAGCAGTCATAAGCTTAGAAAACAAGTGGGGTGCTCTCTCCAAACAAATCCAGATAGCAAATAGGACGGGACATGGACAATTCGAAAACCCTGTTGAGCTATATAACCCTGATGGCACACGATTTAGTGTAACCAATACTTCGGCTGGAGTTGTAAAAGGCAATATCAAACTCCTACTATACTACAAAGCCAGTGTTGGTAGTGAATATGATATCCCTACTACAATCTTGCACCCTGGAGCTATGGGAATGCTTCACAATCAGAATGGAAATTATGTTACAATGTGA

Coding sequence (CDS)

ATGAACAAATTCATAGCACTCTCCTTTTTCTTTCTCGCCATCTCTCTTGGCTCTCCTACTGCCATTGGTGATGTTAGCTTCGATTTGTCGACAGCTACTAAAAAATCCTATTCATCTTTCATCACACAACTCAGGGATGCTCTTCCAACTCAAGGCACAGTGTGCGGCATTCCATTGCTGCCTTCCACCGCATCCGGCTCACAATGGTTCAGATTCTTCAATCTTACCAATTATAACGATGAAACCGTCACCGTGGCTGTAAATGTAACCAATGTCTACATCGTGGCGTATCGTGCCGATGCTGTATCCTACTTTTTTGAAGACACTCCAGCTGAAGCTTTCAAGCTCATATTTGCAGGTACTAAGACGGTAAAACTTCCTTATTCGGGTAATTATGATAAGCTTCAAAGTGTAGTAGGCAAACAAAGAGATATGATTGAGCTTGGAATCCCGGCTTTAAGCAGTGCCATTACAAACATGGTTTATTACGACTACCAAAGTACTGCAGCCGCGCTTCTTGTACTCATTCAGTGTACTGCAGAAGCTGCAAGGTATAAATATATTGAGCAACAAGTTTCTTCACATATTAGCTCTAATTTTTATCCAAATCAAGCAGTCATAAGCTTAGAAAACAAGTGGGGTGCTCTCTCCAAACAAATCCAGATAGCAAATAGGACGGGACATGGACAATTCGAAAACCCTGTTGAGCTATATAACCCTGATGGCACACGATTTAGTGTAACCAATACTTCGGCTGGAGTTGTAAAAGGCAATATCAAACTCCTACTATACTACAAAGCCAGTGTTGGTAGTGAATATGATATCCCTACTACAATCTTGCACCCTGGAGCTATGGGAATGCTTCACAATCAGAATGGAAATTATGTTACAATGTGA

Protein sequence

MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM

Homology

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BLAST of Tan0004757 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 579.7 bits (1493), Expect = 1.9e-164
Identity = 293/294 (99.66%), Postives = 294/294 (100.00%), Query Frame = 0


Query: 5   IALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 64
           +ALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 124
           SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 184
           KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 244
           YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM 299
           FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM 294

[Close]

BLAST of Tan0004757 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 296.2 bits (757), Expect = 4.1e-79
Identity = 157/280 (56.07%), Postives = 201/280 (71.79%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           M K + LSF  +AI +G PTA GDV+FDLSTAT K+Y+ FI   R  LP    V  IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
            ST S S+ F   NLT+Y  ET++VA++VTNVY+VAYR   VSYFF+++P EA+ ++F G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTA 180
           T+ + LPY+GNY+ LQ+   K R+ I+LG+PALSSAIT + YY+ QS  +ALLVLIQ TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNP 240
           EAAR+KYIE+ V+ ++++NF PN A+ISLEN+W ALSKQI +A   G G+F NPV+L  P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 DGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTIL 281
            G RF VTN  + VVKGNIKLLL  +AS   E  I T  L
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTL 279

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BLAST of Tan0004757 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.6e-78
Identity = 156/280 (55.71%), Postives = 201/280 (71.79%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           M K + LSF  +AI +G PTA GDV+FDLSTAT K+Y+ FI   R  LP    V  IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
            ST S S+ F   +LT+Y  ET++VA++VTNVY+VAYR   VSYFF+++P EA+ ++F G
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTA 180
           T+ + LPY+GNY+ LQ+   K R+ I+LG+PALSSAIT + YY+ QS  +ALLVLIQ TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNP 240
           EAAR+KYIE+ V+ ++++NF PN A+ISLEN+W ALSKQI +A   G G+F NPV+L  P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 DGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTIL 281
            G RF VTN  + VVKGNIKLLL  +AS   E  I T  L
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTL 279

[Close]

BLAST of Tan0004757 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 266.5 bits (680), Expect = 3.5e-70
Identity = 146/280 (52.14%), Postives = 186/280 (66.43%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           M +F+ LS   L + L +P   GDVSF LS AT  SY  FI+ LR ALP +  +  IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPA-EAFKLIFA 120
            S+  GSQ +   +LTNY DET++VA++VTNVYI+ YRA   SYFF +  A EA K +F 
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 -GTKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQC 180
              + V LPYSGNY++LQ+  GK R+ I LG+PAL SAIT + YY+  S A+AL+VLIQ 
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELY 240
           T+EAARYK+IEQQ+   +   F P+ A+ISLEN W ALSKQIQIA+ T +GQFE+PV L 
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIAS-TNNGQFESPVVLI 240

Query: 241 NPDGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTT 279
           N    R ++TN  AGVV  NI LLL        + D+P T
Sbjct: 241 NAQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVPMT 279

[Close]

BLAST of Tan0004757 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 262.7 bits (670), Expect = 5.1e-69
Identity = 145/278 (52.16%), Postives = 182/278 (65.47%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           M +F+  S   L + L +P   GDVSF LS AT  SY  FI+ LR ALP +  +  IPLL
Sbjct: 1   MIRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKLYDIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPA-EAFKLIFA 120
            ST  GSQ +   +LTNY DET++VA++VTNVY++ YRA   SYFF +  A EA K +F 
Sbjct: 61  RSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 GTK-TVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQC 180
             K  V LPYSGNY++LQ   GK R+ I LG+PAL SAIT + YY+  S A+AL+VLIQ 
Sbjct: 121 DAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELY 240
           T+EAARYK+IEQQ+   +   F P+ A+ISLEN W ALSKQIQIA+ T +GQFE PV L 
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIAS-TNNGQFETPVVLI 240

Query: 241 NPDGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIP 277
           N    R ++TN  AGVV  NI LLL        + D+P
Sbjct: 241 NAQNQRVTITNVDAGVVTSNIALLLNRNNMAAIDDDVP 277

[Close]

BLAST of Tan0004757 vs. NCBI nr
Match: P56626.2 (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Trichosanthes anguina] >AAD02686.1 trichoanguin [Trichosanthes cucumerina])

HSP 1 Score: 579.7 bits (1493), Expect = 1.4e-161
Identity = 293/294 (99.66%), Postives = 294/294 (100.00%), Query Frame = 0


Query: 5   IALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 64
           +ALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 124
           SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 184
           KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 244
           YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM 299
           FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM 294

[Close]

BLAST of Tan0004757 vs. NCBI nr
Match: XP_023005055.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima])

HSP 1 Score: 411.4 bits (1056), Expect = 6.7e-111
Identity = 209/277 (75.45%), Postives = 238/277 (85.92%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA++ F +AISL SPT   D++FDLSTATK SYS+FITQLR+ALPT  TVC IPLL
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
           PSTASG  WF FF +TNYN +T+TVAVNVTNVYIVAYRADA SYFFEDTPAEA +L+F  
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTA 180
           T+ V LP++GNYDKLQS+VGK RD+IELGIPALS+A+TNMVYY+ Q TAAALLVLIQ +A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNP 240
           EAAR+KYIEQQV+ HIS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 DGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 278
           +GTRFSVT+TSAGVV+ NIKLLLYYK SV  E D PT
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPT 277

[Close]

BLAST of Tan0004757 vs. NCBI nr
Match: KAG6592904.1 (Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025309.1 Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 409.5 bits (1051), Expect = 2.6e-110
Identity = 207/277 (74.73%), Postives = 240/277 (86.64%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA++ F LAISL SPT   D++FDLSTATK SYS+FITQLR+ALPT+ TVC IPLL
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
           PSTA+G  WF FF +TNYN +TV VAVNV+NVYIVAYRADA SYFFEDTPA+A +L+FAG
Sbjct: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTA 180
           T+ V LP++GNYDKLQS+VGK RDM+ELGIPALS+A+TNMVYY+ Q TAAALLVLIQ TA
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180

Query: 181 EAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNP 240
           EAAR+KYIEQQV+ +IS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 DGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 278
           +GTRF VT+TS+GVV+GNIKLLLYYK SV  + D PT
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPT 277

[Close]

BLAST of Tan0004757 vs. NCBI nr
Match: XP_023513430.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-108
Identity = 207/277 (74.73%), Postives = 234/277 (84.48%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA+S F LAISL SPT   D++ DLSTATK SYS+FIT +R+ALPT+ TVC IPLL
Sbjct: 1   MNKLIAISSFLLAISLISPTLKADIALDLSTATKASYSAFITHIRNALPTKATVCNIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
           PST +G   F FF +TNYN +TV VAVNVTNVYIVAYRA A SYFFEDTPA+A +L+FAG
Sbjct: 61  PSTPTGLASFTFFTITNYNYQTVEVAVNVTNVYIVAYRAGASSYFFEDTPAQAKQLLFAG 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTA 180
           T+ V LP++GNYDKLQS+VGK RDM+ELGIPALS+A+TNMVYY+ Q TAAALLVLIQ TA
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNP 240
           EAAR+KYIEQQV+ HIS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL  P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGP 240

Query: 241 DGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 278
            GTRFSVTNTSAGVV+GNIKLLLYYK SV  + D PT
Sbjct: 241 TGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDNDSPT 277

[Close]

BLAST of Tan0004757 vs. NCBI nr
Match: XP_022156709.1 (type I ribosome-inactivating protein trichoanguina-like [Momordica charantia])

HSP 1 Score: 396.0 bits (1016), Expect = 2.9e-106
Identity = 208/286 (72.73%), Postives = 234/286 (81.82%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           M K IALSFF L I LG PTA GDVSFDLSTATK SY+SFI  LR ALPT+GTVC IP+L
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
           PSTASG QWFRFF+LTNYN +T+TVAVNVTNVYI AY   AVSYFFEDT AEAF+L+F G
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVY-YDYQSTAAALLVLIQCT 180
           T+ V LPYSGNYD+LQS+VGK RD+IELGIP LS+AITN+ Y  +Y+ TA ALLVLIQ T
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYN 240
           AEAAR+KYIEQ+VS H+SSNF+PNQA+ISLEN WGALSKQIQIAN  G GQF+  VEL  
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PDGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAM 286
            DG R SVTNTS GVVK NIKLLLYYK +  ++  IP TIL  GA+
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIP-TILPLGAI 285

[Close]

BLAST of Tan0004757 vs. ExPASy TrEMBL
Match: A0A6J1KWC4 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 3.3e-111
Identity = 209/277 (75.45%), Postives = 238/277 (85.92%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA++ F +AISL SPT   D++FDLSTATK SYS+FITQLR+ALPT  TVC IPLL
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
           PSTASG  WF FF +TNYN +T+TVAVNVTNVYIVAYRADA SYFFEDTPAEA +L+F  
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTA 180
           T+ V LP++GNYDKLQS+VGK RD+IELGIPALS+A+TNMVYY+ Q TAAALLVLIQ +A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNP 240
           EAAR+KYIEQQV+ HIS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 DGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 278
           +GTRFSVT+TSAGVV+ NIKLLLYYK SV  E D PT
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPT 277

[Close]

BLAST of Tan0004757 vs. ExPASy TrEMBL
Match: A0A6J1DUC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.4e-106
Identity = 208/286 (72.73%), Postives = 234/286 (81.82%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           M K IALSFF L I LG PTA GDVSFDLSTATK SY+SFI  LR ALPT+GTVC IP+L
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
           PSTASG QWFRFF+LTNYN +T+TVAVNVTNVYI AY   AVSYFFEDT AEAF+L+F G
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVY-YDYQSTAAALLVLIQCT 180
           T+ V LPYSGNYD+LQS+VGK RD+IELGIP LS+AITN+ Y  +Y+ TA ALLVLIQ T
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYN 240
           AEAAR+KYIEQ+VS H+SSNF+PNQA+ISLEN WGALSKQIQIAN  G GQF+  VEL  
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PDGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAM 286
            DG R SVTNTS GVVK NIKLLLYYK +  ++  IP TIL  GA+
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIP-TILPLGAI 285

[Close]

BLAST of Tan0004757 vs. ExPASy TrEMBL
Match: A0A6J1H951 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.9e-104
Identity = 199/277 (71.84%), Postives = 233/277 (84.12%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           MNK IA++ F LAISL SPT   D++ DLSTATK +YS+FITQLR+ALPT+ TVC IPLL
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
           PSTA+G   F FF +TNYN ETV VAVNV+NVYIVAYRA+A SYFFEDT A+A +L+F  
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTA 180
           T+ + LP++GNYD+LQ VVGK RD IELGIPALS+A+TNMVYY+ Q TAAALLVLIQ TA
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNP 240
           EAAR+KYIEQQV+ +IS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 DGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 278
           +GTRF VT+TS+GVV+GNIKLLLYYK SV  + D PT
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPT 277

[Close]

BLAST of Tan0004757 vs. ExPASy TrEMBL
Match: A0A6J1DR40 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 2.3e-93
Identity = 187/298 (62.75%), Postives = 226/298 (75.84%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           M K   LS   LAI LG  +A GDVSF LS A KKSY++FI QLRDA+PT  TVC IP+L
Sbjct: 1   MRKLTTLS-LLLAILLGPLSAEGDVSFQLSGANKKSYTNFIKQLRDAVPTTTTVCKIPVL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
           PSTASG + F F +LTNY D+TV VA+N+TNVYIVAY  D VSYFF+D   EA+K++F G
Sbjct: 61  PSTASGPKSFTFVHLTNYADQTVVVAINITNVYIVAYHIDHVSYFFDDASPEAYKVLFEG 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTA 180
           T    LPYS NYDKLQS+VGK+RD+IELG+PALSSAITN+VYY+Y+STAAALLVLIQ  A
Sbjct: 121 TDRKTLPYSSNYDKLQSIVGKKRDLIELGLPALSSAITNLVYYEYKSTAAALLVLIQSVA 180

Query: 181 EAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNP 240
           EAARYKYIEQ++S+H+   F P+QA+ISLEN WGALSKQIQIA + G+GQFE PVEL NP
Sbjct: 181 EAARYKYIEQEISTHVDDKFKPDQAIISLENNWGALSKQIQIAAK-GNGQFEKPVELINP 240

Query: 241 DGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTILHPGAMGMLHNQNGNYVTM 299
           + T FSVTN S+ VV  NIKLLLY+K +  +  D   T+  P      +N   N++T+
Sbjct: 241 NNTPFSVTNVSSLVVTSNIKLLLYFKLAAANHDDQDKTVGKP-----FNNGGENFITV 291

[Close]

BLAST of Tan0004757 vs. ExPASy TrEMBL
Match: A0A6J1DRC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023553 PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 1.2e-76
Identity = 157/280 (56.07%), Postives = 201/280 (71.79%), Query Frame = 0


Query: 1   MNKFIALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLL 60
           M K + LSF  +AI +G PTA GDV+FDLSTAT K+Y+ FI   R  LP    V  IPLL
Sbjct: 1   MMKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTASGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAG 120
            ST S S+ F   NLT+Y  ET++VA++VTNVY+VAYR   VSYFF+++P EA+ ++F G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TKTVKLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTA 180
           T+ + LPY+GNY+ LQ+   K R+ I+LG+PALSSAIT + YY+ QS  +ALLVLIQ TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNP 240
           EAAR+KYIE+ V+ ++++NF PN A+ISLEN+W ALSKQI +A   G G+F NPV+L  P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 DGTRFSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPTTIL 281
            G RF VTN  + VVKGNIKLLL  +AS   E  I T  L
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADENFITTMTL 279

The following BLAST results are available for this feature:

BLAST of Tan0004757 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P56626	1.9e-164	99.66	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]
P24817	4.1e-79	56.07	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	1.6e-78	55.71	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P09989	3.5e-70	52.14	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P24478	5.1e-69	52.16	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]

BLAST of Tan0004757 vs. NCBI nr


Match Name	E-value	Identity	Description
P56626.2	1.4e-161	99.66	RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt...	[more]
XP_023005055.1	6.7e-111	75.45	type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima]	[more]
KAG6592904.1	2.6e-110	74.73	Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrospe...	[more]
XP_023513430.1	1.1e-108	74.73	type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. p...	[more]
XP_022156709.1	2.9e-106	72.73	type I ribosome-inactivating protein trichoanguina-like [Momordica charantia]	[more]

BLAST of Tan0004757 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KWC4	3.3e-111	75.45	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1	[more]
A0A6J1DUC7	1.4e-106	72.73	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1	[more]
A0A6J1H951	3.9e-104	71.84	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1	[more]
A0A6J1DR40	2.3e-93	62.75	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1	[more]
A0A6J1DRC7	1.2e-76	56.07	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023553 PE=3 SV=1	[more]

BLAST of Tan0004757 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 35..48 score: 45.49 coord: 82..97 score: 51.32 coord: 172..192 score: 63.28 coord: 143..157 score: 51.75 coord: 204..221 score: 51.02
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..221 e-value: 2.7E-33 score: 115.3
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 24..264
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..183 e-value: 7.5E-46 score: 157.7
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 184..270 e-value: 1.1E-28 score: 100.9
None	No IPR available	PIRSR	PIRSR001924-1	PIRSR001924-1	coord: 6..217 e-value: 5.7E-6 score: 23.7
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 24..264
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..271

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0004757.1	Tan0004757.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity