Tan0004348 (gene) Snake gourd v1

Overview


Name	Tan0004348
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	methionine gamma-lyase-like
Location	LG01: 27475881 .. 27480402 (+)
RNA-Seq Expression	Tan0004348
Synteny	Tan0004348

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATTGTACTTTTTTCAATTTCGAAAATAAAAAGTTCTCTCTCTTTTCCTTTTAATTCCATGGCTGAATTCAAAAACCAAACTTTCTGCAGCGCAAAAAGACTGGCCAACGATGAAACCGACGAGAATTTGGTGTCGAAGAAACCAACGGCCATGATTTCGTCTCTCCTCGAAGATCCAGCGGCTGCGATTGCCAACACGCGTCACGAATTCGGCGAACACGGAGGGGTAAATATGTCAATTGAAGCCTCCGCCACATTCACCGTCATGGAACCGGAGACCATGCGGCGGATGTTCGCCGGCGAGCTCGGCCCGGATCGCGACTTCTTCATCTACAGTCGTCATTTCAATCCCACCGTGTTGAATCTGAGCCGTCAGATGGCCGCCCTAGAAGGCACCGCCGCCGCCTACTGCACGTCGAGCGGAATGTCCGCCATCGCCGCAGCGCTGCTGCAGCTCCTCGCCAGTGGGGACCACGTCGTGGCGGCTAGAACCCTCTACGGTGGGACCCACGCCCTGTTGGCCCACTTTTTGCCGAGGACGTCGAATATAACGACGTCGTTTGTGGACATAGGTGATTTGGAGATGGTGGAGAAGGAGATCGTTGAAGGGAAGACGAAAGTGCTTTATTTTGAAGCGGTTGCGAATCCGACTTTGGCGGTGGCGAATATACCGGAGTTGTGTCGGATTGGACATGAGAAGGGGCTGACGGTGGTGGTTGATAATACTTTTGCGCCGATGATTCTCTCGCCGGCGAGGCTCGGTGCTGACGTGGTCGTTCATAGCATTTCTAAGTTCATTAGCGGCGGTGCTGACATCATTGCAGGTACTTTTTCTTCTTTTGTGTTAAAAGTTTTCTGACATTTTGCCTCACTACCCAAATTTTCTTCAAGCCATTTCAACGTCACATAATTTTATATATTTTGTTCTTTTAAAAAATTTATTTGATACAGATTTTAGTTACAGCCTAATTGCACCTAAATTTTTTTCGTTATTAGCCACGTCAGCAATATTTAAGATTTTTTTTTTTTTTTTTTTGTTAATTGAGTAAATAATTTTCCTTTTTAACTAATAGGGATTTCCTACTATAACGGCAGAATATAAGTCTTTTTAACAGAAAATTATGTGGAGGGAGAGAAGAGAGAATATTGAGTAGGGGTGATCCTTGATCGATCGGGTCGGCTTTTGACACCGAGCTCGGCCGGTCGGGTCAGCTTTTTGACACTGAGCTTGGCTGAATAATTTTGAATAATTTTTTTTCCTACCAGTCTATTTAATTATTTATAATTATTATTTGATTATTACTTAATATATTAGATCTAATAGGAAAATCTTATAGTTTTTATTTTTTAATTATTATTATTAATATATATATTTAAATCCGTCGACCACCGACGGCAAAATCGACCCCAATCGAAAAGACACAACCGACCGACTTTGATTTGGTCGGTTTCGATGGGTCGAGTCAGTTTTGTTTTTTTTTTCACCCATAATATTTGGCTGAAAAAAAGAAAAAAAAAATATGGAGTCATCAACAACTCCTAAATATTGCCCCATAGGAATCTACCCATCTATTATTTAATTGTGAGTGTGTGACAACAAAGAAATTATACAAAATATATTATATATTCTCTCTTCTTCATGTCAAGAATTAAATGATGAGGTATGAAGATTCCGATCTATATGCCACTAAATAATATTGAAAGACATTTTTTTTTTCTTTTCTAAGTACTCCTCTTTTGAAGGATGATTTAGATGAGTATTATAAGGATTTGAAAAGGAGCAAAACTTGGTGCAACCACTTATTTCTATGCATCCCTCCATTTAAACAATAAAAATAAAAAAATAATTTGCTACATGAAATGAGTGGACTCATAAAAATGGATGCATGAGATGCAAAGTTTTTTTCTTTGAAAATTTTTACTCTAATGTTCCATGACATCATTGATAGAGCCTAGAGGTACTATTTTGTCTAGTTGTGTTCCGTGACTATTGGAAAAAGTGGGAAGTAATATCATATTGCATTCAAATTTGTTGAATTCAAATCTCTCTTTCCTTAATTAAAAATATATGCCTTAATTTATTTGTTGAGGAAAATATTTTGATGACAACATTCAAATATGGTGTAACCTCTTTTATGGCCACACACAAAAAAAAATTGTGTATATTTTTTTTTTTTTTGGGAAAAGAAAGAAATATGTAGAGAGCACATATTTAAATAAATAAAAAAACACTCTAAATAGTAAGACTAAAATATAGGTTCTACTCTAAGTTAAAGGTTTATTTTTCTATGATTTGAAAAAAGAAAACAAAATCCCACCAAACACACAATGTAAATACTGTCATAGAGTTTTTTTTTTTTTTTTTAATGCAAAATTTTATCATAAACTAATTGCAGAGAGAGTAAATTTAGAAATTTGGGCGTATTTTTACCCATAGTATATGTCAATTAACTGAAACTAAAATTTAAAATCCAGAAATGCCTTTTTTTATTCTTCATTCTTTTTTATGACGAAAGAAGTTCAAAGCTTCTTTTTTTTAAAAAAAAGAAAAGAAAAAGAAACTCGAACTAAAACAAAAAAATCAGATATATTGGTGGGGGGGATGGGTCATGTCACGTGATCATGGTTTTTTTTTTTTTTTTGTAGTACAACAGATGATGGGTTTTGTGGTTGGTTCTTTGTCGTGTTCATATGTCTTCCTAAACGTGTCACCACTTTTTCTAAAAGTGCTTTAATTGGGATAGAGATGGATGTCTCTTTTTTCTGCTTTAAATTTAATACTGAATCATATATAAAGTTAGGAAGATTCCCGATATAAGATTAAAATGACTAGAAATCTCTTTTAAGTGGTCAAACTCAATGCCAATTGATATATACATATATATATATATCCTCAATCAAAATATTAGATGTTCGCATATGTATTATTTCAATATATTCACATGACAACAATTATAATATTTGTATTCAAGGGGAAGAACATGACCTGCAAAACAAGAAAAACCTGCACAGATGTGGTGTTTGCCACACCGTCTCCGATTGTTTAAGTCGGAAAACCTTTTAGAAATGTAGCAAGGAATGAAGATCTATCTCTCTCCCCTCTTAGAAAGAGAGCTTAAATACTCTTAGGATTTTTGGTAGATTCCTTGTAAGAATTGAACGAAAGAGAATCGGATCGGACTGGACGACGAAGATCCTTTGGACTGAAAGGAGTAGACAAAAGTCCAGGTCTTGAGGTGGGGTCGACCTCGACCCTAGACATTTGGGCTTCGACCCAATTATTCTTTCGCTCCTTGCAGTGATTTCGACTCATTGTGATATGTATTTAGCTTTTTAGTTTTGTTCATCCTATAATTTTTGTGGTTTTTTTGGTCGGTCTTTGTAGTTTAATTTCACACGTAACAAGTATAATCGAATGGGGGCTAGGAACGAATGTTAAAAGAATAAAATAATGTAAGTTTTCAAATGTTGACTTTAATAATAATGATGTAACATGATATATAATTAAATCAAGTAGAGAGAGCATATTTTGAATGAAAAATTATGATAAAACTGGATGAGATGAGATGTGCAGGTGCGGTGTGTGGACCAACAAAGCTTGTGAACTCAATGATGGACTTGCAACAAGGGAGCTTGATGCTTCTAGGGCCAACAATGAATGCAAAGGTAGCATTCGAGCTGTCAGAGAGAATCCCTCACCTAAGCCTGAGAATGAAAGAACACTGCAGAAGAGCAGCAGTGTTTGCAGAGAGGATGAAGAAGTTAGGGTTGAAGGTCATATACCCGGGGCTACCCGACCACCCGCAACACCAACTAATGAAGAGCCTCACGAACCCAGAATACGGGTTCGGAGGAATGTTGTGTCTCGACATGGGGACCGAGGAAAGGGCGAATAAGCTCATGAATATACTCCAAAACACCTCACAATTTGGGTTCATGGCAGTGAGTTTAGGGTACTACGAGACCCTAATGTCATGCTCAGGCAGCAGCACAAGCAGTGAAATGAGTGTAGAGGAGAAGGAATTGGCTGGAATCTCACCAGGACTCATAAGAATGTCCATTGGATATATGGGCACATTGGACCAAAAATGGACCCAATTTGAAAAGGCTCTTGCCAAACTGCAAGATGTGGGGCACCTTCCATTTTGCAACAATATTTAATTCATTTATTACCAAATCTAATCTTGAACCCTAATTGTGTGTGTGTGTGTGTGTATATTATAGGTTGGAGCTTGGATATGATGGACTTGGTTTCATCTTGCTCATATAGTTGCTTATCAGTTATGATCTCTCTCTCTGATCCTTTTTTTTTTTTTTTAATTTTCAAATTTTCCTTAAGTAATAAAGAAACATTTGCCATTAACACAAAAGAGTGATGGCCAAAATGTACTTTGAATTAGGCAAATGATGTTTATATTGTTGGGTTATTTTAGTTGAACAATTAAATGAGTTGAATATTGTTTACATATTGGCATGGTTGAAGGTAAGATGAAAATTTTGCCTACAAAAATAGTAAGGT

mRNA sequence

AAAAATTGTACTTTTTTCAATTTCGAAAATAAAAAGTTCTCTCTCTTTTCCTTTTAATTCCATGGCTGAATTCAAAAACCAAACTTTCTGCAGCGCAAAAAGACTGGCCAACGATGAAACCGACGAGAATTTGGTGTCGAAGAAACCAACGGCCATGATTTCGTCTCTCCTCGAAGATCCAGCGGCTGCGATTGCCAACACGCGTCACGAATTCGGCGAACACGGAGGGGTAAATATGTCAATTGAAGCCTCCGCCACATTCACCGTCATGGAACCGGAGACCATGCGGCGGATGTTCGCCGGCGAGCTCGGCCCGGATCGCGACTTCTTCATCTACAGTCGTCATTTCAATCCCACCGTGTTGAATCTGAGCCGTCAGATGGCCGCCCTAGAAGGCACCGCCGCCGCCTACTGCACGTCGAGCGGAATGTCCGCCATCGCCGCAGCGCTGCTGCAGCTCCTCGCCAGTGGGGACCACGTCGTGGCGGCTAGAACCCTCTACGGTGGGACCCACGCCCTGTTGGCCCACTTTTTGCCGAGGACGTCGAATATAACGACGTCGTTTGTGGACATAGGTGATTTGGAGATGGTGGAGAAGGAGATCGTTGAAGGGAAGACGAAAGTGCTTTATTTTGAAGCGGTTGCGAATCCGACTTTGGCGGTGGCGAATATACCGGAGTTGTGTCGGATTGGACATGAGAAGGGGCTGACGGTGGTGGTTGATAATACTTTTGCGCCGATGATTCTCTCGCCGGCGAGGCTCGGTGCTGACGTGGTCGTTCATAGCATTTCTAAGTTCATTAGCGGCGGTGCTGACATCATTGCAGGTGCGGTGTGTGGACCAACAAAGCTTGTGAACTCAATGATGGACTTGCAACAAGGGAGCTTGATGCTTCTAGGGCCAACAATGAATGCAAAGGTAGCATTCGAGCTGTCAGAGAGAATCCCTCACCTAAGCCTGAGAATGAAAGAACACTGCAGAAGAGCAGCAGTGTTTGCAGAGAGGATGAAGAAGTTAGGGTTGAAGGTCATATACCCGGGGCTACCCGACCACCCGCAACACCAACTAATGAAGAGCCTCACGAACCCAGAATACGGGTTCGGAGGAATGTTGTGTCTCGACATGGGGACCGAGGAAAGGGCGAATAAGCTCATGAATATACTCCAAAACACCTCACAATTTGGGTTCATGGCAGTGAGTTTAGGGTACTACGAGACCCTAATGTCATGCTCAGGCAGCAGCACAAGCAGTGAAATGAGTGTAGAGGAGAAGGAATTGGCTGGAATCTCACCAGGACTCATAAGAATGTCCATTGGATATATGGGCACATTGGACCAAAAATGGACCCAATTTGAAAAGGCTCTTGCCAAACTGCAAGATGTGGGGCACCTTCCATTTTGCAACAATATTTAATTCATTTATTACCAAATCTAATCTTGAACCCTAATTGTGTGTGTGTGTGTGTGTATATTATAGGTTGGAGCTTGGATATGATGGACTTGGTTTCATCTTGCTCATATAGTTGCTTATCAGTTATGATCTCTCTCTCTGATCCTTTTTTTTTTTTTTTAATTTTCAAATTTTCCTTAAGTAATAAAGAAACATTTGCCATTAACACAAAAGAGTGATGGCCAAAATGTACTTTGAATTAGGCAAATGATGTTTATATTGTTGGGTTATTTTAGTTGAACAATTAAATGAGTTGAATATTGTTTACATATTGGCATGGTTGAAGGTAAGATGAAAATTTTGCCTACAAAAATAGTAAGGT

Coding sequence (CDS)

ATGGCTGAATTCAAAAACCAAACTTTCTGCAGCGCAAAAAGACTGGCCAACGATGAAACCGACGAGAATTTGGTGTCGAAGAAACCAACGGCCATGATTTCGTCTCTCCTCGAAGATCCAGCGGCTGCGATTGCCAACACGCGTCACGAATTCGGCGAACACGGAGGGGTAAATATGTCAATTGAAGCCTCCGCCACATTCACCGTCATGGAACCGGAGACCATGCGGCGGATGTTCGCCGGCGAGCTCGGCCCGGATCGCGACTTCTTCATCTACAGTCGTCATTTCAATCCCACCGTGTTGAATCTGAGCCGTCAGATGGCCGCCCTAGAAGGCACCGCCGCCGCCTACTGCACGTCGAGCGGAATGTCCGCCATCGCCGCAGCGCTGCTGCAGCTCCTCGCCAGTGGGGACCACGTCGTGGCGGCTAGAACCCTCTACGGTGGGACCCACGCCCTGTTGGCCCACTTTTTGCCGAGGACGTCGAATATAACGACGTCGTTTGTGGACATAGGTGATTTGGAGATGGTGGAGAAGGAGATCGTTGAAGGGAAGACGAAAGTGCTTTATTTTGAAGCGGTTGCGAATCCGACTTTGGCGGTGGCGAATATACCGGAGTTGTGTCGGATTGGACATGAGAAGGGGCTGACGGTGGTGGTTGATAATACTTTTGCGCCGATGATTCTCTCGCCGGCGAGGCTCGGTGCTGACGTGGTCGTTCATAGCATTTCTAAGTTCATTAGCGGCGGTGCTGACATCATTGCAGGTGCGGTGTGTGGACCAACAAAGCTTGTGAACTCAATGATGGACTTGCAACAAGGGAGCTTGATGCTTCTAGGGCCAACAATGAATGCAAAGGTAGCATTCGAGCTGTCAGAGAGAATCCCTCACCTAAGCCTGAGAATGAAAGAACACTGCAGAAGAGCAGCAGTGTTTGCAGAGAGGATGAAGAAGTTAGGGTTGAAGGTCATATACCCGGGGCTACCCGACCACCCGCAACACCAACTAATGAAGAGCCTCACGAACCCAGAATACGGGTTCGGAGGAATGTTGTGTCTCGACATGGGGACCGAGGAAAGGGCGAATAAGCTCATGAATATACTCCAAAACACCTCACAATTTGGGTTCATGGCAGTGAGTTTAGGGTACTACGAGACCCTAATGTCATGCTCAGGCAGCAGCACAAGCAGTGAAATGAGTGTAGAGGAGAAGGAATTGGCTGGAATCTCACCAGGACTCATAAGAATGTCCATTGGATATATGGGCACATTGGACCAAAAATGGACCCAATTTGAAAAGGCTCTTGCCAAACTGCAAGATGTGGGGCACCTTCCATTTTGCAACAATATTTAA

Protein sequence

MAEFKNQTFCSAKRLANDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKEIVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEERANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGYMGTLDQKWTQFEKALAKLQDVGHLPFCNNI

Homology

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BLAST of Tan0004348 vs. ExPASy Swiss-Prot
Match: Q9SGU9 (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 3.6e-183
Identity = 322/422 (76.30%), Postives = 363/422 (86.02%), Query Frame = 0


Query: 17  NDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFTVMEPETMR 76
           +DE  + LV+KK    +     DPAAAIAN RHEFGEHGGVNMSIEASATFTVMEP+TMR
Sbjct: 22  DDEDGDALVAKKSALAVCD--ADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMR 81

Query: 77  RMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAALLQLLAS 136
           RMF GELGPD DF++YSRHFNPTVLNLSRQMAALEGT AAYCTSSGMSAI++ +LQL +S
Sbjct: 82  RMFTGELGPDNDFYVYSRHFNPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSS 141

Query: 137 GDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKEIVEGKTKVLYFEAVAN 196
           G HVVAA TLYGGTHALL+HFLPRT NITTSFVDI D   V   IVEG+T+VLYFE+VAN
Sbjct: 142 GGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVAN 201

Query: 197 PTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVVHSISKFISGGADIIAG 256
           PTL VA+IPEL R+ HEKG+TVVVDNTFAPM+LSPA+LGADVVVHSISKFISGGADIIAG
Sbjct: 202 PTLTVADIPELSRMAHEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAG 261

Query: 257 AVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEHCRRAAVFAERM 316
           AVCG   LV  MMDL+ GSLMLLGPTMNAKVAFELSERIPHL LRM+EH  RA V+AERM
Sbjct: 262 AVCGSENLVKEMMDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERM 321

Query: 317 KKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEERANKLMNILQNTSQFGF 376
           + LG+KVIYPGL  HPQH+L K + N +YG+GG+L +DM TEE+ANKLM  LQN +QFGF
Sbjct: 322 RDLGMKVIYPGLETHPQHKLFKGMVNRDYGYGGLLSIDMETEEKANKLMAYLQNATQFGF 381

Query: 377 MAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGYMGTLDQKWTQFEKALA 436
           MAVSLGYYETLMSCSGSSTSSE+   +KE AGISPGL+RMS+GY+GTL+QKWTQFEKA  
Sbjct: 382 MAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFL 441

Query: 437 KL 439
           ++
Sbjct: 442 RM 441

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BLAST of Tan0004348 vs. ExPASy Swiss-Prot
Match: Q8L0X4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 GN=mgl PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.2e-51
Identity = 144/390 (36.92%), Postives = 214/390 (54.87%), Query Frame = 0


Query: 54  HGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGT 113
           +G + M I  ++TF     E   R FA E       +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRFALE----EAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 114 AAAYCTSSGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGD 173
            AA  TSSGM AI++ L  +L +GDHVV  +TLYG T AL+ H L R   I  +FVD  +
Sbjct: 82  EAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMCHGLTR-FGIEVTFVDTSN 141

Query: 174 LEMVEKEIVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLT-VVVDNTFA-PMILSP 233
           L+ V K  ++  T+V+Y E  ANP L + ++  L ++ H    T V+VDNTFA P +  P
Sbjct: 142 LDEV-KNAMKKNTRVVYLETPANPNLKIVDLEALSKLAHTNPNTLVIVDNTFATPYMQKP 201

Query: 234 ARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFEL 293
            +LGAD+VVHS++K+I+G  D+IAG V    +L + +  +  G   + G  +  + A+ +
Sbjct: 202 LKLGADIVVHSVTKYINGHGDVIAGLVITNKELADQIRFI--GLKDMTGAVLGPQDAYYI 261

Query: 294 SERIPHLSLRMKEHCRRAAVFAERMKKLGL--KVIYPGLPDHPQHQLMKSLTNPEYGFGG 353
              +    +RM+ HC+ A    E + K     +V YPGL  HP H++ K        FG 
Sbjct: 262 IRGMKTFEIRMERHCKNAKKVVEFLNKHPKIERVYYPGLETHPGHEIAKKQMK---DFGA 321

Query: 354 MLCLDM-GTEERANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAG 413
           M+  ++ G  E    L+N L+  S    +AVSLG  ETL+    S T S  + EE+E AG
Sbjct: 322 MISFELKGGFEAGKTLLNNLKLCS----LAVSLGDTETLIQHPASMTHSPYTKEEREAAG 381

Query: 414 ISPGLIRMSIGYMGTLDQKWTQFEKALAKL 439
           I+ GL+R+S+G +  ++      E+ L K+
Sbjct: 382 ITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

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BLAST of Tan0004348 vs. ExPASy Swiss-Prot
Match: Q8RDT4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.0e-49
Identity = 140/390 (35.90%), Postives = 211/390 (54.10%), Query Frame = 0


Query: 54  HGGVNMSIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGT 113
           +G + M I  ++TF     E   R FA E       +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRFALE----EAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 114 AAAYCTSSGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGD 173
            A    SSGM AI++ L  +L +GDHVV  +TLYG T AL+ H L R   +  +FVD  +
Sbjct: 82  EAGIAMSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMNHGLTR-FGVEVTFVDTSN 141

Query: 174 LEMVEKEIVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLT-VVVDNTFA-PMILSP 233
           LE V K  ++  T+V+Y E  ANP L + ++  L +I H    T V+VDNTFA P +  P
Sbjct: 142 LEEV-KNAMKKNTRVVYLETPANPNLKIVDLEALSKIAHTNPNTLVIVDNTFATPYMQKP 201

Query: 234 ARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFEL 293
            +LG D+VVHS +K+++G  D+IAG V    +L + +  +  G   + G  +  + A+ +
Sbjct: 202 LKLGVDIVVHSATKYLNGHGDVIAGLVVTRQELADQIRFV--GLKDMTGAVLGPQEAYYI 261

Query: 294 SERIPHLSLRMKEHCRRAAVFAERMKKLGL--KVIYPGLPDHPQHQLMKSLTNPEYGFGG 353
              +    +RM+ HC+ A    + + K     KV YPGL  HP +++ K        FG 
Sbjct: 262 IRGLKTFEIRMERHCKNARTIVDFLNKHPKVEKVYYPGLETHPGYEIAKKQMK---DFGA 321

Query: 354 MLCLDM-GTEERANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAG 413
           M+  ++ G  E    L+N L+  S    +AVSLG  ETL+    S T S  + EE+E+AG
Sbjct: 322 MISFELKGGFEAGKTLLNNLKLCS----LAVSLGDTETLIQHPASMTHSPYTKEEREVAG 381

Query: 414 ISPGLIRMSIGYMGTLDQKWTQFEKALAKL 439
           I+ GL+R+S+G +  ++      E+ L K+
Sbjct: 382 ITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

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BLAST of Tan0004348 vs. ExPASy Swiss-Prot
Match: Q7MX71 (L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=mgl PE=1 SV=2)

HSP 1 Score: 196.1 bits (497), Expect = 8.8e-49
Identity = 140/365 (38.36%), Postives = 201/365 (55.07%), Query Frame = 0


Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           I  ++TF     E   R FAGE     D +IY+R  NP    +  ++A LEG  AA   S
Sbjct: 32  IYQTSTFVFDTAEQGGRRFAGE----EDGYIYTRLGNPNCTQVEEKLAMLEGGEAAASAS 91

Query: 121 SGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKE 180
           SG+ AI++A+   + +GDH+VA +TLYG T A L H L R   +  + VD    E VE  
Sbjct: 92  SGIGAISSAIWVCVKAGDHIVAGKTLYGCTFAFLTHGLSR-YGVEVTLVDTRHPEEVEAA 151

Query: 181 IVEGKTKVLYFEAVANPTLAVANIPELCRIGHE-KGLTVVVDNTF-APMILSPARLGADV 240
           I    TK++Y E  ANP + + +I  +C I H+ +G+ V+VDNT+  P I  P  LGAD+
Sbjct: 152 I-RPNTKLVYLETPANPNMYLTDIKAVCDIAHKHEGVRVMVDNTYCTPYICRPLELGADI 211

Query: 241 VVHSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHL 300
           VVHS +K+++G  D+IAG V G    +  +  +  G   L G  M+   A+ +S  +  L
Sbjct: 212 VVHSATKYLNGHGDVIAGFVVGKEDYIKEVKLV--GVKDLTGANMSPFDAYLISRGMKTL 271

Query: 301 SLRMKEHCRRAAVFAERMKKLGL--KVIYPGLPDHPQHQL-MKSLTNPEYGFGGMLCLDM 360
            +RM++HCR A   AE ++K      V +PGLP  PQ++L  K +  P    G M+  ++
Sbjct: 272 QIRMEQHCRNAQTVAEFLEKHPAVEAVYFPGLPSFPQYELAKKQMALP----GAMIAFEV 331

Query: 361 -GTEERANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLI 420
            G  E   KLMN L   S    +AVSLG  ETL+    S T S  + EE+  + IS GL+
Sbjct: 332 KGGCEAGKKLMNNLHLCS----LAVSLGDTETLIQHPASMTHSPYTPEERAASDISEGLV 380

[Close]

BLAST of Tan0004348 vs. ExPASy Swiss-Prot
Match: P13254 (L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 2.2e-47
Identity = 147/390 (37.69%), Postives = 207/390 (53.08%), Query Frame = 0


Query: 53  EHGGVNM-SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALE 112
           +HGG  +  +  +ATFT    E     FAGE    +    YSR  NPT+  L  +MA+LE
Sbjct: 23  DHGGALVPPVYQTATFTFPTVEYGAACFAGE----QAGHFYSRISNPTLNLLEARMASLE 82

Query: 113 GTAAAYCTSSGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDI 172
           G  A    +SGM AI + L  LL  GD V+   TLYG T A L H +     +    VD+
Sbjct: 83  GGEAGLALASGMGAITSTLWTLLRPGDEVLLGNTLYGCTFAFLHHGIGE-FGVKLRHVDM 142

Query: 173 GDLEMVEKEIVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTF-APMILS 232
            DL+ +E  +    T+V+YFE+ ANP + +A+I  + +I  + G TVVVDNT+  P +  
Sbjct: 143 ADLQALEAAMTPA-TRVIYFESPANPNMHMADIAGVAKIARKHGATVVVDNTYCTPYLQR 202

Query: 233 PARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFE 292
           P  LGAD+VVHS +K++SG  DI AG V G   LV+ +    QG   + G  ++   A  
Sbjct: 203 PLELGADLVVHSATKYLSGHGDITAGIVVGSQALVDRIR--LQGLKDMTGAVLSPHDAAL 262

Query: 293 LSERIPHLSLRMKEHCRRAAVFAE---RMKKLGLKVIYPGLPDHPQHQLMK-SLTNPEYG 352
           L   I  L+LRM  HC  A V AE   R  ++ L + YPGL   PQ+ L +  ++ P   
Sbjct: 263 LMRGIKTLNLRMDRHCANAQVLAEFLARQPQVEL-IHYPGLASFPQYTLARQQMSQP--- 322

Query: 353 FGGMLCLDM-GTEERANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKE 412
            GGM+  ++ G      + MN LQ  S+    AVSLG  E+L     S T S  + EE+ 
Sbjct: 323 -GGMIAFELKGGIGAGRRFMNALQLFSR----AVSLGDAESLAQHPASMTHSSYTPEERA 382

Query: 413 LAGISPGLIRMSIGYMGTLDQKWTQFEKAL 436
             GIS GL+R+S+G +  +D      ++AL
Sbjct: 383 HYGISEGLVRLSVG-LEDIDDLLADVQQAL 394

[Close]

BLAST of Tan0004348 vs. NCBI nr
Match: KAG6596676.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 819.3 bits (2115), Expect = 1.6e-233
Identity = 412/450 (91.56%), Postives = 433/450 (96.22%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE KNQ  CSAKR A  ETDENL+SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNSCSAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKE 180
           SGMSAIAA  LQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDI DLEMVEK 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVV 240
           IVEGKTKVLYFEA+ANPTL VANIPELCRIGHEKGLTVVVDNTFAPM+LSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSL 300
           HSISKFISGGADIIAGAVCGPTKLVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLSL
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKS+ N EYGFGG+LCLDMGTEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGY 420
           ANKLMN+LQNT+QFGF+AVSLGYYETLMSCSGSSTSSE+S ++KELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLDQKWTQFEKALAKLQDVGHLPFCNNI 451
           MGTL+QKW+QFEKALAK+QD+G LP+CNNI
Sbjct: 421 MGTLEQKWSQFEKALAKVQDLG-LPYCNNI 449

[Close]

BLAST of Tan0004348 vs. NCBI nr
Match: XP_023538834.1 (methionine gamma-lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 817.4 bits (2110), Expect = 6.2e-233
Identity = 411/450 (91.33%), Postives = 432/450 (96.00%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE KNQ  C AKR A  ETDENL+SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQKSCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKE 180
           SGMSAIAA  LQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDI DLEMVEK 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVV 240
           IVEGKTKVLYFEA+ANPTL VANIPELCRIGHEKGLTVVVDNTFAPM+LSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSL 300
           HSISKFISGGADIIAGAVCGPTKLVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLSL
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKS+ N EYGFGG+LCLDMGTEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGY 420
           ANKLMN+LQNT+QFGF+AVSLGYYETLMSCSGSSTSSE+S ++KELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLDQKWTQFEKALAKLQDVGHLPFCNNI 451
           MGTL+QKW+QFEKALAK+QD+G LP+CNNI
Sbjct: 421 MGTLEQKWSQFEKALAKVQDLG-LPYCNNI 449

[Close]

BLAST of Tan0004348 vs. NCBI nr
Match: KAG7028211.1 (Methionine gamma-lyase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 816.2 bits (2107), Expect = 1.4e-232
Identity = 410/450 (91.11%), Postives = 431/450 (95.78%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE KNQ  C AKR A  ETDENL+SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNSCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKE 180
           SGMSAIAA  LQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDI DLEMVEK 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVV 240
           IVEGKTKVLYFEA+ANPTL VANIPELCRIGHEKGLTVVVDNTFAPM+LSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSL 300
           HSISKFISGGADIIAGAVCGPTKLVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLSL
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKS+ N EYGFGG+LCLDMGTEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGY 420
           ANKLMN+LQNT+QFGF+AVSLGYYETLMSCSGSSTSSE+S ++KELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLDQKWTQFEKALAKLQDVGHLPFCNNI 451
           MGTL+QKW+QFEKAL K+QD+G LP+CNNI
Sbjct: 421 MGTLEQKWSQFEKALTKVQDLG-LPYCNNI 449

[Close]

BLAST of Tan0004348 vs. NCBI nr
Match: XP_022950227.1 (methionine gamma-lyase-like [Cucurbita moschata])

HSP 1 Score: 813.1 bits (2099), Expect = 1.2e-231
Identity = 408/450 (90.67%), Postives = 431/450 (95.78%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE KNQ  C AKR A  ETDENL+SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNSCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKE 180
           SGMSAIAA  LQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDI DLEMVEK 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVV 240
           IVEGKTKVLYFEA+ANPTL VANIPELCRIGHEKGLTVVVDNTFAPM+LSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSL 300
           HSISKFISGGADIIAGAVCGPTKLVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLSL
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQL+KS+ N EYGFGG+LCLDMGTEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLLKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGY 420
           ANKLMN+LQNT+QFGF+AVSLGYYETLMSCSGSSTSSE+S ++KELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLDQKWTQFEKALAKLQDVGHLPFCNNI 451
           MGTL+QKW+QFEKAL K+Q++G LP+CNNI
Sbjct: 421 MGTLEQKWSQFEKALTKVQNLG-LPYCNNI 449

[Close]

BLAST of Tan0004348 vs. NCBI nr
Match: XP_023005861.1 (methionine gamma-lyase-like [Cucurbita maxima])

HSP 1 Score: 812.8 bits (2098), Expect = 1.5e-231
Identity = 410/450 (91.11%), Postives = 429/450 (95.33%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE KNQ FC AKR A  ETDENL+SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNFCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGP+RDFFIYSRHFNPTVLNLSR +AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPERDFFIYSRHFNPTVLNLSRLIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKE 180
           SGMSAIAA  LQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDI DLEMVEK 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVV 240
           IVEGKTKVLYFEA+ANPTL VANIPELCRIGHEKGLTVVVDNTFAPM+LSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSL 300
           HSISKFISGGADIIAGAVCGPTKLVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLSL
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSL N EYG GG+LCLDMGTEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLGNSEYGCGGILCLDMGTEER 360

Query: 361 ANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGY 420
           ANKLMN+LQNT+QFGF+AVSLGYYETLMSCS SSTSSE+S  +KELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSSSSTSSELSGNDKELAGISPGLVRMSIGY 420

Query: 421 MGTLDQKWTQFEKALAKLQDVGHLPFCNNI 451
           MGTL+QKW+QFEKALAK+QD+G LPFCNNI
Sbjct: 421 MGTLEQKWSQFEKALAKVQDLG-LPFCNNI 449

[Close]

BLAST of Tan0004348 vs. ExPASy TrEMBL
Match: A0A6J1GE83 (methionine gamma-lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111453379 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 5.6e-232
Identity = 408/450 (90.67%), Postives = 431/450 (95.78%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE KNQ  C AKR A  ETDENL+SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNSCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGPDRDFFIYSRHFNPTVLNLSRQ+AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPDRDFFIYSRHFNPTVLNLSRQIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKE 180
           SGMSAIAA  LQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDI DLEMVEK 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVV 240
           IVEGKTKVLYFEA+ANPTL VANIPELCRIGHEKGLTVVVDNTFAPM+LSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSL 300
           HSISKFISGGADIIAGAVCGPTKLVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLSL
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQL+KS+ N EYGFGG+LCLDMGTEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLLKSIGNSEYGFGGILCLDMGTEER 360

Query: 361 ANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGY 420
           ANKLMN+LQNT+QFGF+AVSLGYYETLMSCSGSSTSSE+S ++KELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSGSSTSSELSGKDKELAGISPGLVRMSIGY 420

Query: 421 MGTLDQKWTQFEKALAKLQDVGHLPFCNNI 451
           MGTL+QKW+QFEKAL K+Q++G LP+CNNI
Sbjct: 421 MGTLEQKWSQFEKALTKVQNLG-LPYCNNI 449

[Close]

BLAST of Tan0004348 vs. ExPASy TrEMBL
Match: A0A6J1KW67 (methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111498738 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 7.3e-232
Identity = 410/450 (91.11%), Postives = 429/450 (95.33%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60
           MAE KNQ FC AKR A  ETDENL+SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS
Sbjct: 1   MAECKNQNFCGAKRPATGETDENLISKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMS 60

Query: 61  IEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTS 120
           IEASATFTVMEPETM+R+FAGELGP+RDFFIYSRHFNPTVLNLSR +AALEGTAAAYCT+
Sbjct: 61  IEASATFTVMEPETMQRLFAGELGPERDFFIYSRHFNPTVLNLSRLIAALEGTAAAYCTA 120

Query: 121 SGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKE 180
           SGMSAIAA  LQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDI DLEMVEK 
Sbjct: 121 SGMSAIAAVFLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDISDLEMVEKA 180

Query: 181 IVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVV 240
           IVEGKTKVLYFEA+ANPTL VANIPELCRIGHEKGLTVVVDNTFAPM+LSPARLGADVVV
Sbjct: 181 IVEGKTKVLYFEAIANPTLVVANIPELCRIGHEKGLTVVVDNTFAPMVLSPARLGADVVV 240

Query: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSL 300
           HSISKFISGGADIIAGAVCGPTKLVNSMMDL QG LMLLGPTMNAKVAFELSERIPHLSL
Sbjct: 241 HSISKFISGGADIIAGAVCGPTKLVNSMMDLLQGGLMLLGPTMNAKVAFELSERIPHLSL 300

Query: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEER 360
           RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSL N EYG GG+LCLDMGTEER
Sbjct: 301 RMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLGNSEYGCGGILCLDMGTEER 360

Query: 361 ANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGY 420
           ANKLMN+LQNT+QFGF+AVSLGYYETLMSCS SSTSSE+S  +KELAGISPGL+RMSIGY
Sbjct: 361 ANKLMNVLQNTTQFGFIAVSLGYYETLMSCSSSSTSSELSGNDKELAGISPGLVRMSIGY 420

Query: 421 MGTLDQKWTQFEKALAKLQDVGHLPFCNNI 451
           MGTL+QKW+QFEKALAK+QD+G LPFCNNI
Sbjct: 421 MGTLEQKWSQFEKALAKVQDLG-LPFCNNI 449

[Close]

BLAST of Tan0004348 vs. ExPASy TrEMBL
Match: A0A5A7UHF4 (Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00790 PE=3 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.5e-229
Identity = 407/450 (90.44%), Postives = 429/450 (95.33%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDE-NLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60
           MAE KN  F   KR + DE+D+    SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM
Sbjct: 1   MAELKNHNFRGTKRASPDESDDVKTDSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60

Query: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120
           SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT
Sbjct: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120

Query: 121 SSGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEK 180
           SSGMSAIAA LLQLLASGDHVVA+RTLYGGTHAL+AHF PRTSNITT+FVDIGDL+ VEK
Sbjct: 121 SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDIGDLKEVEK 180

Query: 181 EIVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVV 240
            IVEGKTKVLYFE+V+NPTLAVANIPELCRIGHEKG+TVVVDNTFAPMILSPARLGADVV
Sbjct: 181 AIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSPARLGADVV 240

Query: 241 VHSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLS 300
           VHSISKFISGGADIIAGAVCGPTKLVNSMMDL+QGSLMLLGPTMNAKVAFELSERIPHLS
Sbjct: 241 VHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS 300

Query: 301 LRMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEE 360
           LRMKEHCRRA+VFAERMKK GLKVIYPGL DHPQHQLMKSL NPEYGFGGMLC+DMGTEE
Sbjct: 301 LRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGMLCVDMGTEE 360

Query: 361 RANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIG 420
           RANKLM+ILQNT+QFGFMAVSLGYYETLMSCSGSSTSSEMS EE+ELAGISPGL+RMSIG
Sbjct: 361 RANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISPGLVRMSIG 420

Query: 421 YMGTLDQKWTQFEKALAKLQDVGHLPFCNN 450
           YMGTL+QKW+QFEKALAK+QD+G +PFCNN
Sbjct: 421 YMGTLEQKWSQFEKALAKVQDIG-VPFCNN 449

[Close]

BLAST of Tan0004348 vs. ExPASy TrEMBL
Match: A0A1S3BKU9 (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103490704 PE=3 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.5e-229
Identity = 407/450 (90.44%), Postives = 429/450 (95.33%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDE-NLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60
           MAE KN  F   KR + DE+D+    SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM
Sbjct: 1   MAELKNHNFRGTKRASPDESDDVKTDSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60

Query: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120
           SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT
Sbjct: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120

Query: 121 SSGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEK 180
           SSGMSAIAA LLQLLASGDHVVA+RTLYGGTHAL+AHF PRTSNITT+FVDIGDL+ VEK
Sbjct: 121 SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDIGDLKEVEK 180

Query: 181 EIVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVV 240
            IVEGKTKVLYFE+V+NPTLAVANIPELCRIGHEKG+TVVVDNTFAPMILSPARLGADVV
Sbjct: 181 AIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSPARLGADVV 240

Query: 241 VHSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLS 300
           VHSISKFISGGADIIAGAVCGPTKLVNSMMDL+QGSLMLLGPTMNAKVAFELSERIPHLS
Sbjct: 241 VHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS 300

Query: 301 LRMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEE 360
           LRMKEHCRRA+VFAERMKK GLKVIYPGL DHPQHQLMKSL NPEYGFGGMLC+DMGTEE
Sbjct: 301 LRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGMLCVDMGTEE 360

Query: 361 RANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIG 420
           RANKLM+ILQNT+QFGFMAVSLGYYETLMSCSGSSTSSEMS EE+ELAGISPGL+RMSIG
Sbjct: 361 RANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISPGLVRMSIG 420

Query: 421 YMGTLDQKWTQFEKALAKLQDVGHLPFCNN 450
           YMGTL+QKW+QFEKALAK+QD+G +PFCNN
Sbjct: 421 YMGTLEQKWSQFEKALAKVQDIG-VPFCNN 449

[Close]

BLAST of Tan0004348 vs. ExPASy TrEMBL
Match: M1NFB7 (Methionine gamma-lyase OS=Cucumis melo OX=3656 GN=MGL PE=2 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 7.6e-229
Identity = 407/450 (90.44%), Postives = 429/450 (95.33%), Query Frame = 0


Query: 1   MAEFKNQTFCSAKRLANDETDE-NLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60
           MAE KN  F   KR + DE+D+    SKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM
Sbjct: 1   MAELKNHNFRGTKRPSPDESDDVKTDSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNM 60

Query: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120
           SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT
Sbjct: 61  SIEASATFTVMEPETMRRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCT 120

Query: 121 SSGMSAIAAALLQLLASGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEK 180
           SSGMSAIAA LLQLLASGDHVVA+RTLYGGTHAL+AHF PRTSNITT+FVDIGDL+ VEK
Sbjct: 121 SSGMSAIAAVLLQLLASGDHVVASRTLYGGTHALMAHFFPRTSNITTTFVDIGDLKEVEK 180

Query: 181 EIVEGKTKVLYFEAVANPTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVV 240
            IVEGKTKVLYFE+V+NPTLAVANIPELCRIGHEKG+TVVVDNTFAPMILSPARLGADVV
Sbjct: 181 AIVEGKTKVLYFESVSNPTLAVANIPELCRIGHEKGVTVVVDNTFAPMILSPARLGADVV 240

Query: 241 VHSISKFISGGADIIAGAVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLS 300
           VHSISKFISGGADIIAGAVCGPTKLVNSMMDL+QGSLMLLGPTMNAKVAFELSERIPHLS
Sbjct: 241 VHSISKFISGGADIIAGAVCGPTKLVNSMMDLRQGSLMLLGPTMNAKVAFELSERIPHLS 300

Query: 301 LRMKEHCRRAAVFAERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEE 360
           LRMKEHCRRA+VFAERMKK GLKVIYPGL DHPQHQLMKSL NPEYGFGGMLC+DMGTEE
Sbjct: 301 LRMKEHCRRASVFAERMKKAGLKVIYPGLEDHPQHQLMKSLANPEYGFGGMLCVDMGTEE 360

Query: 361 RANKLMNILQNTSQFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIG 420
           RANKLM+ILQNT+QFGFMAVSLGYYETLMSCSGSSTSSEMS EE+ELAGISPGL+RMSIG
Sbjct: 361 RANKLMSILQNTTQFGFMAVSLGYYETLMSCSGSSTSSEMSGEERELAGISPGLVRMSIG 420

Query: 421 YMGTLDQKWTQFEKALAKLQDVGHLPFCNN 450
           YMGTL+QKW+QFEKALAK+QD+G +PFCNN
Sbjct: 421 YMGTLEQKWSQFEKALAKVQDIG-VPFCNN 449

[Close]

BLAST of Tan0004348 vs. TAIR 10
Match: AT1G64660.1 (methionine gamma-lyase )

HSP 1 Score: 642.5 bits (1656), Expect = 2.5e-184
Identity = 322/422 (76.30%), Postives = 363/422 (86.02%), Query Frame = 0


Query: 17  NDETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFTVMEPETMR 76
           +DE  + LV+KK    +     DPAAAIAN RHEFGEHGGVNMSIEASATFTVMEP+TMR
Sbjct: 22  DDEDGDALVAKKSALAVCD--ADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMR 81

Query: 77  RMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAALLQLLAS 136
           RMF GELGPD DF++YSRHFNPTVLNLSRQMAALEGT AAYCTSSGMSAI++ +LQL +S
Sbjct: 82  RMFTGELGPDNDFYVYSRHFNPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSS 141

Query: 137 GDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKEIVEGKTKVLYFEAVAN 196
           G HVVAA TLYGGTHALL+HFLPRT NITTSFVDI D   V   IVEG+T+VLYFE+VAN
Sbjct: 142 GGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVAN 201

Query: 197 PTLAVANIPELCRIGHEKGLTVVVDNTFAPMILSPARLGADVVVHSISKFISGGADIIAG 256
           PTL VA+IPEL R+ HEKG+TVVVDNTFAPM+LSPA+LGADVVVHSISKFISGGADIIAG
Sbjct: 202 PTLTVADIPELSRMAHEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAG 261

Query: 257 AVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEHCRRAAVFAERM 316
           AVCG   LV  MMDL+ GSLMLLGPTMNAKVAFELSERIPHL LRM+EH  RA V+AERM
Sbjct: 262 AVCGSENLVKEMMDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERM 321

Query: 317 KKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEERANKLMNILQNTSQFGF 376
           + LG+KVIYPGL  HPQH+L K + N +YG+GG+L +DM TEE+ANKLM  LQN +QFGF
Sbjct: 322 RDLGMKVIYPGLETHPQHKLFKGMVNRDYGYGGLLSIDMETEEKANKLMAYLQNATQFGF 381

Query: 377 MAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIGYMGTLDQKWTQFEKALA 436
           MAVSLGYYETLMSCSGSSTSSE+   +KE AGISPGL+RMS+GY+GTL+QKWTQFEKA  
Sbjct: 382 MAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFL 441

Query: 437 KL 439
           ++
Sbjct: 442 RM 441

[Close]

BLAST of Tan0004348 vs. TAIR 10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.2e-39
Identity = 110/335 (32.84%), Postives = 175/335 (52.24%), Query Frame = 0


Query: 90  FIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAALLQLLASGDHVVAARTLYGG 149
           F Y R+ NPT + L  +++ALEG  +    +SGM A    LL L+ +G H+V     Y  
Sbjct: 224 FEYGRYGNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRK 283

Query: 150 THALLAHFLPRTSNITTSFVDIGDLEMVEKEIVEGKTKVLYFEAVANPTLAVANIPELCR 209
           T   + +FLP+   IT + +D  D+  +E  + E K  + + E+  NP L   +I  + +
Sbjct: 284 TRIFMENFLPKL-GITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSK 343

Query: 210 IGHEKGLTVVVDNTFA-PMILSPARLGADVVVHSISKFISGGADIIAGAVCGPTKLVNSM 269
           I H++G  V +D TFA P+      LGAD+VVHS +K+I G  D++AG +CG  KLV+ +
Sbjct: 344 ICHKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEI 403

Query: 270 MDLQQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEH---CRRAAVFAERMKKLGLKVIY 329
            +L      +LG T+N   A+ +   +  L LR+++      R A   E   K+   V Y
Sbjct: 404 RNLHH----VLGGTLNPNAAYLIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVS-HVYY 463

Query: 330 PGLPDHPQHQLMKSLTNPEYGFGGMLCLDM-GTEERANKLMNILQNTSQFGFMAVSLGYY 389
           PGLP HP+H+L K       GFGG++  ++ G  E   K ++ L    +  ++A S G  
Sbjct: 464 PGLPSHPEHELAKRQMT---GFGGVVSFEIDGDIETTIKFVDSL----KIPYIAPSFGGC 523

Query: 390 ETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIG 420
           E+++      +  ++  EE+   GI   L+R S G
Sbjct: 524 ESIVDQPAIMSYWDLPQEERLKYGIKDNLVRFSFG 545

[Close]

BLAST of Tan0004348 vs. TAIR 10
Match: AT3G57050.1 (cystathionine beta-lyase )

HSP 1 Score: 137.5 bits (345), Expect = 2.6e-32
Identity = 119/407 (29.24%), Postives = 209/407 (51.35%), Query Frame = 0


Query: 17  NDETDE-NLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFTVMEPETM 76
           N+ TD  N ++ K  A +S+LL        N  ++F     ++  +  +ATF        
Sbjct: 70  NNTTDSLNTMNIKEEASVSTLL-------VNLDNKFDPFDAMSTPLYQTATF-------- 129

Query: 77  RRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAALLQLLA 136
           ++  A E GP    + Y+R  NPT   L   +A L+    A+C +SGM+A+ +A+  L+ 
Sbjct: 130 KQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAAL-SAVTHLIK 189

Query: 137 SGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKEIVEGKTKVLYFEAVA 196
           +G+ +VA   +YGG+  LL+  +PR S +    V+   L+ V   I   +TK+++ E+  
Sbjct: 190 NGEEIVAGDDVYGGSDRLLSQVVPR-SGVVVKRVNTTKLDEVAAAI-GPQTKLVWLESPT 249

Query: 197 NPTLAVANIPELCRIGHEKGLTVVVDNT-FAPMILSPARLGADVVVHSISKFISGGADII 256
           NP   +++I ++  + H +G  V+VDN+  +P++  P  LGAD+V+HS +KFI+G +D++
Sbjct: 250 NPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVM 309

Query: 257 AG--AVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEHCRRAAVF 316
           AG  AV G  KL   +  LQ      L P  +  +     + +     + +E+ R+ A++
Sbjct: 310 AGVLAVKG-EKLAKEVYFLQNSEGSGLAP-FDCWLCLRGIKTMALRIEKQQENARKIAMY 369

Query: 317 AERMKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEERANKLMNILQNTS 376
                ++  KV Y GLPDHP H L  S      G G +     G+   +  L+     T+
Sbjct: 370 LSSHPRV-KKVYYAGLPDHPGHHLHFSQAK---GAGSVFSFITGSVALSKHLV----ETT 429

Query: 377 QFGFMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIG 420
           ++  +AVS G  ++L+S     + + +  E +E  G++  L+R+S G
Sbjct: 430 KYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAG 444

[Close]

BLAST of Tan0004348 vs. TAIR 10
Match: AT3G57050.2 (cystathionine beta-lyase )

HSP 1 Score: 134.8 bits (338), Expect = 1.7e-31
Identity = 116/404 (28.71%), Postives = 206/404 (50.99%), Query Frame = 0


Query: 19  ETDENLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFTVMEPETMRRM 78
           E   + ++ K  A +S+LL        N  ++F     ++  +  +ATF        ++ 
Sbjct: 58  EKSVDAMNIKEEASVSTLL-------VNLDNKFDPFDAMSTPLYQTATF--------KQP 117

Query: 79  FAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAALLQLLASGD 138
            A E GP    + Y+R  NPT   L   +A L+    A+C +SGM+A+ +A+  L+ +G+
Sbjct: 118 SAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAAL-SAVTHLIKNGE 177

Query: 139 HVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKEIVEGKTKVLYFEAVANPT 198
            +VA   +YGG+  LL+  +PR S +    V+   L+ V   I   +TK+++ E+  NP 
Sbjct: 178 EIVAGDDVYGGSDRLLSQVVPR-SGVVVKRVNTTKLDEVAAAI-GPQTKLVWLESPTNPR 237

Query: 199 LAVANIPELCRIGHEKGLTVVVDNT-FAPMILSPARLGADVVVHSISKFISGGADIIAG- 258
             +++I ++  + H +G  V+VDN+  +P++  P  LGAD+V+HS +KFI+G +D++AG 
Sbjct: 238 QQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVMAGV 297

Query: 259 -AVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEHCRRAAVFAER 318
            AV G  KL   +  LQ      L P  +  +     + +     + +E+ R+ A++   
Sbjct: 298 LAVKG-EKLAKEVYFLQNSEGSGLAP-FDCWLCLRGIKTMALRIEKQQENARKIAMYLSS 357

Query: 319 MKKLGLKVIYPGLPDHPQHQLMKSLTNPEYGFGGMLCLDMGTEERANKLMNILQNTSQFG 378
             ++  KV Y GLPDHP H L  S      G G +     G+   +  L+     T+++ 
Sbjct: 358 HPRV-KKVYYAGLPDHPGHHLHFSQAK---GAGSVFSFITGSVALSKHLV----ETTKYF 417

Query: 379 FMAVSLGYYETLMSCSGSSTSSEMSVEEKELAGISPGLIRMSIG 420
            +AVS G  ++L+S     + + +  E +E  G++  L+R+S G
Sbjct: 418 SIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAG 429

[Close]

BLAST of Tan0004348 vs. TAIR 10
Match: AT3G57050.3 (cystathionine beta-lyase )

HSP 1 Score: 121.7 bits (304), Expect = 1.5e-27
Identity = 100/324 (30.86%), Postives = 171/324 (52.78%), Query Frame = 0


Query: 17  NDETDE-NLVSKKPTAMISSLLEDPAAAIANTRHEFGEHGGVNMSIEASATFTVMEPETM 76
           N+ TD  N ++ K  A +S+LL        N  ++F     ++  +  +ATF        
Sbjct: 70  NNTTDSLNTMNIKEEASVSTLL-------VNLDNKFDPFDAMSTPLYQTATF-------- 129

Query: 77  RRMFAGELGPDRDFFIYSRHFNPTVLNLSRQMAALEGTAAAYCTSSGMSAIAAALLQLLA 136
           ++  A E GP    + Y+R  NPT   L   +A L+    A+C +SGM+A+ +A+  L+ 
Sbjct: 130 KQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSGMAAL-SAVTHLIK 189

Query: 137 SGDHVVAARTLYGGTHALLAHFLPRTSNITTSFVDIGDLEMVEKEIVEGKTKVLYFEAVA 196
           +G+ +VA   +YGG+  LL+  +PR S +    V+   L+ V   I   +TK+++ E+  
Sbjct: 190 NGEEIVAGDDVYGGSDRLLSQVVPR-SGVVVKRVNTTKLDEVAAAI-GPQTKLVWLESPT 249

Query: 197 NPTLAVANIPELCRIGHEKGLTVVVDNT-FAPMILSPARLGADVVVHSISKFISGGADII 256
           NP   +++I ++  + H +G  V+VDN+  +P++  P  LGAD+V+HS +KFI+G +D++
Sbjct: 250 NPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVM 309

Query: 257 AG--AVCGPTKLVNSMMDLQQGSLMLLGPTMNAKVAFELSERIPHLSLRMKEHCRRAAVF 316
           AG  AV G  KL   +  LQ      L P  +  +     + +     + +E+ R+ A++
Sbjct: 310 AGVLAVKG-EKLAKEVYFLQNSEGSGLAP-FDCWLCLRGIKTMALRIEKQQENARKIAMY 368

Query: 317 AERMKKLGLKVIYPGLPDHPQHQL 337
                ++  KV Y GLPDHP H L
Sbjct: 370 LSSHPRV-KKVYYAGLPDHPGHHL 368

The following BLAST results are available for this feature:

BLAST of Tan0004348 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGU9	3.6e-183	76.30	Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1	[more]
Q8L0X4	3.2e-51	36.92	L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 ...	[more]
Q8RDT4	4.0e-49	35.90	L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC...	[more]
Q7MX71	8.8e-49	38.36	L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83)...	[more]
P13254	2.2e-47	37.69	L-methionine gamma-lyase OS=Pseudomonas putida OX=303 GN=mdeA PE=1 SV=2	[more]

BLAST of Tan0004348 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596676.1	1.6e-233	91.56	Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023538834.1	6.2e-233	91.33	methionine gamma-lyase-like [Cucurbita pepo subsp. pepo]	[more]
KAG7028211.1	1.4e-232	91.11	Methionine gamma-lyase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022950227.1	1.2e-231	90.67	methionine gamma-lyase-like [Cucurbita moschata]	[more]
XP_023005861.1	1.5e-231	91.11	methionine gamma-lyase-like [Cucurbita maxima]	[more]

BLAST of Tan0004348 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GE83	5.6e-232	90.67	methionine gamma-lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111453379 PE=3 S...	[more]
A0A6J1KW67	7.3e-232	91.11	methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111498738 PE=3 SV=...	[more]
A0A5A7UHF4	4.5e-229	90.44	Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BKU9	4.5e-229	90.44	methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103490704 PE=3 SV=1	[more]
M1NFB7	7.6e-229	90.44	Methionine gamma-lyase OS=Cucumis melo OX=3656 GN=MGL PE=2 SV=1	[more]

BLAST of Tan0004348 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64660.1	2.5e-184	76.30	methionine gamma-lyase	[more]
AT3G01120.1	2.2e-39	32.84	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]
AT3G57050.1	2.6e-32	29.24	cystathionine beta-lyase	[more]
AT3G57050.2	1.7e-31	28.71	cystathionine beta-lyase	[more]
AT3G57050.3	1.5e-27	30.86	cystathionine beta-lyase	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PIRSF	PIRSF001434	CGS	coord: 27..439 e-value: 3.6E-102 score: 340.0
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PFAM	PF01053	Cys_Met_Meta_PP	coord: 48..420 e-value: 8.6E-83 score: 278.0
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PANTHER	PTHR11808	TRANS-SULFURATION ENZYME FAMILY MEMBER	coord: 1..439
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 299..438 e-value: 1.8E-26 score: 95.0
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 41..295 e-value: 3.5E-66 score: 225.0
None	No IPR available	PIRSR	PIRSR001434-2	PIRSR001434-2	coord: 93..399 e-value: 4.5E-80 score: 266.9
None	No IPR available	PANTHER	PTHR11808:SF76	METHIONINE GAMMA-LYASE-LIKE	coord: 1..439
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 51..426

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0004348.1	Tan0004348.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019343	cysteine biosynthetic process via cystathionine
biological_process	GO:0019346	transsulfuration
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004123	cystathionine gamma-lyase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0003824	catalytic activity