Tan0003704 (gene) Snake gourd v1

BLAST of Tan0003704 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 1.1e-305
Identity = 488/652 (74.85%), Postives = 577/652 (88.50%), Query Frame = 0

Query: 3   QMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGNRM 62
           Q KY+ +VE+ K+  DGRPS+GPVYRSIFAKDGFP PI+G+DSCWD+FR+SVE+ P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 63  LGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISMEA 122
           LGRREIV+GK GKYVW TY+EVYD+V+K+GNSL + G ++ AKCGIYGAN  EWIISMEA
Sbjct: 64  LGRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEA 123

Query: 123 CNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVSFG 182
           CNAHGLYCVPLYDTLGA AVEFII HSE+SI FVEEKK+ E+ KT PN+T+Y+KT+VSFG
Sbjct: 124 CNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFG 183

Query: 183 KVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMI 242
            V+   +EE E +GL I++WDEFL LGE + +DLP+KKKSDI TIMYTSGTTGDPKGVMI
Sbjct: 184 GVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMI 243

Query: 243 SNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGD 302
           SN+SI++LIAGV RLL++ NE L  KDVYLSYLPLAHIFDRVIEE FI HGA+IGFWRGD
Sbjct: 244 SNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGD 303

Query: 303 VKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRKGH 362
           VKLL+ED+ ELKP+IFCAVPRVLDR+Y+GL +KL  GG  KK +F+ A+S+KF  M+KG 
Sbjct: 304 VKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQ 363

Query: 363 KHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTET 422
            H EASP+ DK+VFSKVK+GLGGNVR+ILSGAAPLA+HVE+FLRVVAC HVLQGYGLTE+
Sbjct: 364 SHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTES 423

Query: 423 CAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYY 482
           CAGTFVSLP+EL MLGTVGPPVPNVD+ LESVPEM YDAL++T RGEIC+RG  LFSGYY
Sbjct: 424 CAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYY 483

Query: 483 KRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVS 542
           KR+DLT EV+IDGW HTGD+GEWQPDGS+KIIDRKKNIFKLSQGEYVAVEN+ENIYG V 
Sbjct: 484 KREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQ 543

Query: 543 SLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELNKI 602
           ++D +WVYGNSFESFL+A+ANPN+  LERWA EN + GD+D+LC++++AK+++LGEL K+
Sbjct: 544 AVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKM 603

Query: 603 AKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMY 655
           AKEKK+KGFE IKAIHLDP+PFDMERDL+TPT+KKKRPQLLKYYQ+VID MY
Sbjct: 604 AKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMY 655

BLAST of Tan0003704 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 4.3e-302
Identity = 482/658 (73.25%), Postives = 561/658 (85.26%), Query Frame = 0

Query: 2   SQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGNR 61
           SQ +++ EVE  K+A DG PS+GPVYRS FA++GFP PIDG+ SCWDIFR +VE+ P NR
Sbjct: 3   SQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNR 62

Query: 62  MLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISME 121
           MLGRREI NGKAGKYVW TYKEVYD+VIK+GNSL + G +EG KCGIYG NC EWIISME
Sbjct: 63  MLGRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISME 122

Query: 122 ACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVSF 181
           ACNAHGLYCVPLYDTLGAGAVEFII H+E+SIAFVEEKK+PE+ KT PN+TKY+KT+VSF
Sbjct: 123 ACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSF 182

Query: 182 GKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVM 241
           G V P  +EE EK GL I SWDEFL LGE + ++LP+KK SDI TIMYTSGTTGDPKGVM
Sbjct: 183 GGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVM 242

Query: 242 ISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRG 301
           ISN+SI+++  GV   L NVN  L+EKDVY+SYLPLAH+FDR IEE  I  G SIGFWRG
Sbjct: 243 ISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRG 302

Query: 302 DVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRKG 361
           DVKLL+ED+GELKPSIFCAVPRVLDR+Y GL QKL  GG FKK +F++A+S+KF NM+KG
Sbjct: 303 DVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKG 362

Query: 362 HKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTE 421
             H  ASP CDK+VF+KVK+GLGGNVR+ILSGAAPLA+H+E+FLRVVAC +VLQGYGLTE
Sbjct: 363 QSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTE 422

Query: 422 TCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGY 481
           +CAGTF + P+EL MLGTVGPPVPNVD+ LESVPEM YDAL +TPRGEIC+RG  LFSGY
Sbjct: 423 SCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGY 482

Query: 482 YKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLV 541
           YKR+DLT EV IDGW HTGD+GEWQP+GS+KIIDRKKNIFKL+QGEYVAVENLEN+Y  V
Sbjct: 483 YKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQV 542

Query: 542 SSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELNK 601
             ++ IWVYGNSFESFLVA+ANP +  LERWA EN + GDF+S+C++ +AK ++LGEL K
Sbjct: 543 EVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVK 602

Query: 602 IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKK 660
            AKE KLKGFE IK +HL+P+ FDMERDL+TPTYKKKRPQLLKYYQNVI  MY  TK+
Sbjct: 603 TAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKE 660

BLAST of Tan0003704 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 9.5e-294
Identity = 470/660 (71.21%), Postives = 561/660 (85.00%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAID-GRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPG 60
           M+  +Y++EVEK K  +D G PS+GPVYRSI+AKDGFP P D L S WDIFRLSVE++P 
Sbjct: 1   MATGRYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPN 60

Query: 61  NRMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIIS 120
           N MLGRREIV+GKAGKYVW TYKEV+++VIK+GNS+   G  +G KCGIYGAN  EWIIS
Sbjct: 61  NPMLGRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIIS 120

Query: 121 MEACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIV 180
           MEACNAHGLYCVPLYDTLGAGA+EFIICH+E+S+AF EE K+ E+LKT P +TKYLK IV
Sbjct: 121 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIV 180

Query: 181 SFGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKG 240
           SFG+VT N R E E++ L I+SWD+FL LGE +H++LP K++SD+ TIMYTSGTTGDPKG
Sbjct: 181 SFGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKG 240

Query: 241 VMISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFW 300
           V+++N+SII L+ GV++LL+ ++EEL  KDVYLSYLPLAHIFDRVIEEL I   ASIGFW
Sbjct: 241 VLLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFW 300

Query: 301 RGDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMR 360
           RGDVK+L+EDI  LKP++FCAVPRVL+RIY GL QKL  GG  KK LFN A+ +K  NM 
Sbjct: 301 RGDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNME 360

Query: 361 KGHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGL 420
           KG  H +ASPI DKIVF KVKEGLGGNVRLILSGAAPLA H+E+FLRVVACAHVLQGYGL
Sbjct: 361 KGQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGL 420

Query: 421 TETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFS 480
           TE+C GTFVS+PNELSMLGTVGPPVPNVD+ LESVPEMGYDAL++ PRGEIC+RG  LFS
Sbjct: 421 TESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFS 480

Query: 481 GYYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 540
           GYYKR+DLT EV IDGW HTGD+GEWQPDG++KIIDRKKNIFKLSQGEYVAVENLENIY 
Sbjct: 481 GYYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYS 540

Query: 541 LVSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGEL 600
            V++++ IWVYGNS+ES+LVAV  P+K+ +E WA E+ + GDF+S+C +++ K+++LGE 
Sbjct: 541 HVAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEF 600

Query: 601 NKIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKK 660
           N++AK+KKLKGFE IK +HLD +PFDMERDLITP+YK KRPQLLKYYQ  ID MY   ++
Sbjct: 601 NRVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYKKNRE 660

BLAST of Tan0003704 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 8.2e-229
Identity = 366/656 (55.79%), Postives = 491/656 (74.85%), Query Frame = 0

Query: 4   MKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGNRML 63
           M Y ++VE+ + A +  PS GPVYRSI+A+DG     +GL S W+    SV+R+P   ML
Sbjct: 1   MSYTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPML 60

Query: 64  GRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISMEAC 123
           GRR+I + +AG YVWLTY+EV+D  I++ +++ +RG   G +CGIYG NC +WI++M+AC
Sbjct: 61  GRRQIKDSEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQAC 120

Query: 124 NAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVSFGK 183
            +H +  VPLYDTLG  AVEFII H E+SIAFV+E K+  +L   PN +  LKTIVSFG 
Sbjct: 121 YSHAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFGN 180

Query: 184 VTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMIS 243
           +T   ++E E  G+  FSW+EF  LG N   +LP K ++DI T+MYTSG TG+PKGV+++
Sbjct: 181 ITSVQKKETEALGVSCFSWEEFSQLG-NLSGELPEKHRTDICTLMYTSGATGEPKGVILT 240

Query: 244 NKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDV 303
           N++I++ I     +LE  ++  +E+DVY SYLPLAH++D+++E   I  G++IG+WRGD+
Sbjct: 241 NEAIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGDI 300

Query: 304 KLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRKGHK 363
           + L++D+ ELKP+IFC VPRV DRIYAG+  K+ +GG  KK LF  AY++K +NM KG  
Sbjct: 301 RFLMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGLP 360

Query: 364 HGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTETC 423
           HG+A+P+ DK+ F K+K+G GG +RL+ SGAAPL  HVE +LRV +CA + QGYGLTE+C
Sbjct: 361 HGQAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTESC 420

Query: 424 AGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYYK 483
            G   S+ N   M+GTVG P+  ++  LESVPEMGYDALS  PRGEIC+RG  LFSGY+K
Sbjct: 421 GGCLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYHK 480

Query: 484 RDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVSS 543
           R+DLT +V++DGWFHTGDIGEWQP+G++KIIDRKKNIFKLSQGEYV VEN+E IY     
Sbjct: 481 REDLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCPL 540

Query: 544 LDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELNKIA 603
           +  IWVYGNSFESFLVAV  P +LALE WA   ++  DF SLCE+ +A  Y+L ELN  A
Sbjct: 541 IASIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELNSTA 600

Query: 604 KEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKK 660
           K+ +L+GFE +KA++L+P PFDMERDLITPT+K KRPQLLKYY++ +D++Y+  K+
Sbjct: 601 KKHQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYKDHVDKLYSEAKE 655

BLAST of Tan0003704 vs. ExPASy Swiss-Prot
Match: Q9XIA9 (Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 8.5e-218
Identity = 353/655 (53.89%), Postives = 478/655 (72.98%), Query Frame = 0

Query: 7   LLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGNRMLGRR 66
           +L VE+ + A    PS GPVYR  +AKDG       +DS W  F  +V++ P  +MLG+R
Sbjct: 8   VLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQR 67

Query: 67  EIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISMEACNAH 126
              + K G Y W+TYKE +D  I+IG+++ +RG + G  CGIYGANC EWII+MEAC + 
Sbjct: 68  VTTDSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQ 127

Query: 127 GLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVSFGKVTP 186
           G+  VPLYD+LG  AVEFII H+E+S+ FV+EK +  +L      +  LKTIVSFG+V+ 
Sbjct: 128 GITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSS 187

Query: 187 NLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMISNKS 246
             +EE +   + +FSW+EF L+G     +LP K+K+DI TIMYTSGTTG+PKGV+++N +
Sbjct: 188 TQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAA 247

Query: 247 IISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKLL 306
           I   +  + ++LE  +   +  DV+ SYLPLAH +D+V+E  F+  G+S+G+WRGD++ L
Sbjct: 248 ISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYL 307

Query: 307 VEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRKGHKHGE 366
           ++D+  LKP++FC VPRV D++YAG+ QK+ A G+ +K LF+ AY++K  NMRKG    E
Sbjct: 308 MDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEE 367

Query: 367 ASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTETCAGT 426
           ASP  D+++F K+KE LGG   ++LSGAAPL  HVE FLR++  +++ QGYGLTE+C G+
Sbjct: 368 ASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGS 427

Query: 427 FVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALST-TPRGEICVRGDPLFSGYYKRD 486
           F +L    SM+GTVG P+P V+  L SVPEMGYDA S   PRGEIC+RG+ +FSGY+KR 
Sbjct: 428 FTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQ 487

Query: 487 DLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVSSLD 546
           DLT++V+IDGWFHTGDIGEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLEN Y     + 
Sbjct: 488 DLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 547

Query: 547 MIWVYGNSFESFLVAVANPNKLALERWANEN-DIEGDFDSLCEDKRAKDYLLGELNKIAK 606
            IWVYGNSFESFLV V  P++ A+E WA  N     DF+SLC++ +A+ Y L ELN  AK
Sbjct: 548 QIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNSTAK 607

Query: 607 EKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKK 660
           + +LKGFE +KAIHL+P PFD+ERDLITPT+K KRPQLL++Y+ ++D++Y+  K+
Sbjct: 608 QYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSEAKR 662

BLAST of Tan0003704 vs. NCBI nr
Match: XP_022931292.1 (long chain acyl-CoA synthetase 4-like [Cucurbita moschata])

HSP 1 Score: 1300.4 bits (3364), Expect = 0.0e+00
Identity = 624/660 (94.55%), Postives = 648/660 (98.18%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL++VE+ K AIDGRPSIGPVYRSIFAKDGFPPPI+GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPPPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSL +RGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EIS+ FVEEKKLPEVLKTFP+TTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKV PNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLE+VNEE+N KDVYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQ+LDAGG F+KTLFNLAYSHK+ NM+K
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHG+ASPICDK+VFSKVK+GLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGDASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VS++DMIWVYGNSFESFLVAVANPNKLALERWANEN IEGDFDSLCEDKRAKDYLLGE+N
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGEIN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+NVID MY N+KKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYKNVIDSMYKNSKKP 660

BLAST of Tan0003704 vs. NCBI nr
Match: XP_038876793.1 (long chain acyl-CoA synthetase 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1299.6 bits (3362), Expect = 0.0e+00
Identity = 627/660 (95.00%), Postives = 644/660 (97.58%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL+EVEKPK AIDGRPSIGPVYRSIFAKDGFPPPI GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIEVEKPKAAIDGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLG REIV+GK GKYVWLTYKEVYD+V+KIGNSLH RGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGHREIVDGKPGKYVWLTYKEVYDIVVKIGNSLHTRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EIS+AFVEEKKLPEVLKTFP+TTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKV PNLREEFEKYG+EIFSWDEF LLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLREEFEKYGVEIFSWDEFSLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLE+VNEEL+ +DVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEELSPEDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQ+LDAGGIFKKTLFN+AYS KF NM+K
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGIFKKTLFNIAYSRKFGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHGEASPICD+ VF+KVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALST PRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTIPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VSS+DMIWVYGNSFESFLVAVANPNKLALERWANEN IE DFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEEDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNN KKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNAKKP 660

BLAST of Tan0003704 vs. NCBI nr
Match: XP_022985381.1 (long chain acyl-CoA synthetase 4-like [Cucurbita maxima])

HSP 1 Score: 1295.0 bits (3350), Expect = 0.0e+00
Identity = 623/660 (94.39%), Postives = 646/660 (97.88%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL++VE+ K AIDGRPSIGPVYRSIFAKDGFP PI+GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPLPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSL +RGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EIS+ FVEEKKLPEVLKTFP+TTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKV PNL+EEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLKEEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLE+VNEE+N KDVYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQ+LDAGG F+KTLFNLAYSHK+ NM+K
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHGEASPICDK+VFSKVK+GLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VS++DMIWVYGNSFESFLVAVANPNKLALERWA EN IEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWAKENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+NVID MY N+KKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYKNVIDSMYKNSKKP 660

BLAST of Tan0003704 vs. NCBI nr
Match: KAG7015143.1 (Long chain acyl-CoA synthetase 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 622/660 (94.24%), Postives = 647/660 (98.03%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL++VE+ K AIDGRPSIGPVYRSIFAKDGFP PI+GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPLPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSL +RGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EIS+ FVEEKKLPEVLKTFP+TTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKV PNL+EEFEKYGLEIFSW+EFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVEPNLKEEFEKYGLEIFSWEEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLE+VNEE+N KDVYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQ+LDAGG F+KTLFNLAYSHK+ NM+K
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHGEASPICDK+VFSKVK+GLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VS++DMIWVYGNSFESFLVAVANPNKLALERWANEN IEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           KI+KEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+NVID MY N+KKP
Sbjct: 601 KISKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYKNVIDSMYKNSKKP 660

BLAST of Tan0003704 vs. NCBI nr
Match: XP_023552641.1 (long chain acyl-CoA synthetase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 621/660 (94.09%), Postives = 647/660 (98.03%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL++VE+ K AIDGRPSIGPVYRSIFAKDGFP PI+GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPLPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSL +RGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGL+CVPLYDTLGAGAVEFIICH+EIS+ FVEEKKLPEVLKTFP+TTKYLKTIVS
Sbjct: 121 EACNAHGLFCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKV PNL+EEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLKEEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLE+VNEE+N KDVYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQ+LDAGG F+KTLFNLAYSHK+ NM+K
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHGEASPICDK+VFSKVK+GLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPN+LSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNKLSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VS++DMIWVYGNSFESFLVAVANPNKLALERWANEN IEGDFD+LCEDKRAKDYLLGELN
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDALCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+NVID MY N+KKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYKNVIDSMYKNSKKP 660

BLAST of Tan0003704 vs. ExPASy TrEMBL
Match: A0A6J1ET87 (long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111437522 PE=4 SV=1)

HSP 1 Score: 1300.4 bits (3364), Expect = 0.0e+00
Identity = 624/660 (94.55%), Postives = 648/660 (98.18%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL++VE+ K AIDGRPSIGPVYRSIFAKDGFPPPI+GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPPPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSL +RGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EIS+ FVEEKKLPEVLKTFP+TTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKV PNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLE+VNEE+N KDVYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQ+LDAGG F+KTLFNLAYSHK+ NM+K
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHG+ASPICDK+VFSKVK+GLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGDASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VS++DMIWVYGNSFESFLVAVANPNKLALERWANEN IEGDFDSLCEDKRAKDYLLGE+N
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGEIN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+NVID MY N+KKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYKNVIDSMYKNSKKP 660

BLAST of Tan0003704 vs. ExPASy TrEMBL
Match: A0A6J1J803 (long chain acyl-CoA synthetase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483406 PE=4 SV=1)

HSP 1 Score: 1295.0 bits (3350), Expect = 0.0e+00
Identity = 623/660 (94.39%), Postives = 646/660 (97.88%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL++VE+ K AIDGRPSIGPVYRSIFAKDGFP PI+GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPLPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSL +RGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EIS+ FVEEKKLPEVLKTFP+TTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKV PNL+EEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLKEEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLE+VNEE+N KDVYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQ+LDAGG F+KTLFNLAYSHK+ NM+K
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHGEASPICDK+VFSKVK+GLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VS++DMIWVYGNSFESFLVAVANPNKLALERWA EN IEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWAKENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+NVID MY N+KKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYKNVIDSMYKNSKKP 660

BLAST of Tan0003704 vs. ExPASy TrEMBL
Match: A0A6J1FMV6 (long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111447265 PE=4 SV=1)

HSP 1 Score: 1292.3 bits (3343), Expect = 0.0e+00
Identity = 625/660 (94.70%), Postives = 643/660 (97.42%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL+EVEK KDAI+GR SIGPVYRSIFAKDGFPPPI GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIEVEKHKDAINGRHSIGPVYRSIFAKDGFPPPIHGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLG REIVNGKAGKYVWLTYKEVYD+VIKIGNSL +RGF EG KCGIYGANCSEWIISM
Sbjct: 61  RMLGHREIVNGKAGKYVWLTYKEVYDIVIKIGNSLRSRGFAEGVKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPE+LKT P+TTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEILKTCPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           +GKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 YGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           +ISNKSIISLIAGVQRLLE+VNE LNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 IISNKSIISLIAGVQRLLESVNESLNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYS KF NM+K
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSRKFGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHGEASPICDK VF+KVKEGLGGNVRLILSGAAPL+THVE FLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKFVFNKVKEGLGGNVRLILSGAAPLSTHVETFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           Y+KRDDLTNEVMIDGWFHTGDIGEWQP+GS+KIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YFKRDDLTNEVMIDGWFHTGDIGEWQPNGSMKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VSS+DMIWVYGNSFESFLVAVANPNKLALERWANEN I+GDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENSIKGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           +IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLK YQNVID MYNN KKP
Sbjct: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKCYQNVIDSMYNNAKKP 660

BLAST of Tan0003704 vs. ExPASy TrEMBL
Match: A0A1S3BTT7 (long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103493094 PE=4 SV=1)

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 618/660 (93.64%), Postives = 640/660 (96.97%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL+EVEKPK AI GRPSIGPVYRSIFAKDGFPPPI GLDSCWDIFRLSVERNP N
Sbjct: 1   MSQMKYLIEVEKPKPAIGGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPDN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRRE VNGKAGKYVWLTYKEVYD V+K+GNSL  RGFE+GAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRRETVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEIS+AFVEEKKLPEVLKTFP+TTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEK+G+EIFSWDEF LLGENQHFDLPVKK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFSLLGENQHFDLPVKKRSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLENVNEEL+ KDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKL++EDIGELKPSIFCAVPRVLDRIYAGLTQ+LD GG+ K+TLFN+AYS K+ NM+K
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNIAYSRKYGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKH EASPICD+ VF+KVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           YYKRDDLTNEVMIDGWFHTGDIGEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VSS+DMIWVYGNSFESFLVAVANPNKLALERWANEN IEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ+VID MYNN KKP
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQSVIDSMYNNAKKP 660

BLAST of Tan0003704 vs. ExPASy TrEMBL
Match: A0A6J1IWU1 (long chain acyl-CoA synthetase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111479187 PE=4 SV=1)

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 622/660 (94.24%), Postives = 641/660 (97.12%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGN 60
           MSQMKYL+EVEK KDAI+GR SIGPVYRSIFAKDGFPPPI GLDSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIEVEKHKDAINGRHSIGPVYRSIFAKDGFPPPIHGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISM 120
           RMLG REIVNGKAGKYVWLTYKEVYD+VIKIGNSL +R F EG KCGIYGANCSEWIISM
Sbjct: 61  RMLGHREIVNGKAGKYVWLTYKEVYDIVIKIGNSLRSRSFAEGVKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPE+LKT P+TTKYLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEILKTCPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240
           +GKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV
Sbjct: 181 YGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           +ISNKSIISL AGVQRLLE+VNE LNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 IISNKSIISLTAGVQRLLESVNESLNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRK 360
           GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKK+LFNLAYSHKF NM+K
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKSLFNLAYSHKFGNMQK 360

Query: 361 GHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420
           GHKHGEASPICDK VF+KVKEGLGGNVRLILSGAAPL+THVE FLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKFVFNKVKEGLGGNVRLILSGAAPLSTHVETFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNE SMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNEFSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540
           Y+KRDDLTNEVMIDGWFHTGDIGEWQP+GS+KIIDRKKNIFKLSQGEYVAVENLENIYGL
Sbjct: 481 YFKRDDLTNEVMIDGWFHTGDIGEWQPNGSMKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELN 600
           VSS+DMIWVYGNSFESFLVAVANPNKLALERWANEN I+GDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENCIKGDFDSLCEDKRAKDYLLGELN 600

Query: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKKP 660
           +IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLK YQNVID MYNN KKP
Sbjct: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKCYQNVIDSMYNNAKKP 660

BLAST of Tan0003704 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1050.0 bits (2714), Expect = 7.7e-307
Identity = 488/652 (74.85%), Postives = 577/652 (88.50%), Query Frame = 0

Query: 3   QMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGNRM 62
           Q KY+ +VE+ K+  DGRPS+GPVYRSIFAKDGFP PI+G+DSCWD+FR+SVE+ P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 63  LGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISMEA 122
           LGRREIV+GK GKYVW TY+EVYD+V+K+GNSL + G ++ AKCGIYGAN  EWIISMEA
Sbjct: 64  LGRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEA 123

Query: 123 CNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVSFG 182
           CNAHGLYCVPLYDTLGA AVEFII HSE+SI FVEEKK+ E+ KT PN+T+Y+KT+VSFG
Sbjct: 124 CNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFG 183

Query: 183 KVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMI 242
            V+   +EE E +GL I++WDEFL LGE + +DLP+KKKSDI TIMYTSGTTGDPKGVMI
Sbjct: 184 GVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMI 243

Query: 243 SNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGD 302
           SN+SI++LIAGV RLL++ NE L  KDVYLSYLPLAHIFDRVIEE FI HGA+IGFWRGD
Sbjct: 244 SNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGD 303

Query: 303 VKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRKGH 362
           VKLL+ED+ ELKP+IFCAVPRVLDR+Y+GL +KL  GG  KK +F+ A+S+KF  M+KG 
Sbjct: 304 VKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQ 363

Query: 363 KHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTET 422
            H EASP+ DK+VFSKVK+GLGGNVR+ILSGAAPLA+HVE+FLRVVAC HVLQGYGLTE+
Sbjct: 364 SHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTES 423

Query: 423 CAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYY 482
           CAGTFVSLP+EL MLGTVGPPVPNVD+ LESVPEM YDAL++T RGEIC+RG  LFSGYY
Sbjct: 424 CAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYY 483

Query: 483 KRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVS 542
           KR+DLT EV+IDGW HTGD+GEWQPDGS+KIIDRKKNIFKLSQGEYVAVEN+ENIYG V 
Sbjct: 484 KREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQ 543

Query: 543 SLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELNKI 602
           ++D +WVYGNSFESFL+A+ANPN+  LERWA EN + GD+D+LC++++AK+++LGEL K+
Sbjct: 544 AVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKM 603

Query: 603 AKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMY 655
           AKEKK+KGFE IKAIHLDP+PFDMERDL+TPT+KKKRPQLLKYYQ+VID MY
Sbjct: 604 AKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQSVIDEMY 655

BLAST of Tan0003704 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1038.1 bits (2683), Expect = 3.0e-303
Identity = 482/658 (73.25%), Postives = 561/658 (85.26%), Query Frame = 0

Query: 2   SQMKYLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGNR 61
           SQ +++ EVE  K+A DG PS+GPVYRS FA++GFP PIDG+ SCWDIFR +VE+ P NR
Sbjct: 3   SQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNR 62

Query: 62  MLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISME 121
           MLGRREI NGKAGKYVW TYKEVYD+VIK+GNSL + G +EG KCGIYG NC EWIISME
Sbjct: 63  MLGRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISME 122

Query: 122 ACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVSF 181
           ACNAHGLYCVPLYDTLGAGAVEFII H+E+SIAFVEEKK+PE+ KT PN+TKY+KT+VSF
Sbjct: 123 ACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSF 182

Query: 182 GKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVM 241
           G V P  +EE EK GL I SWDEFL LGE + ++LP+KK SDI TIMYTSGTTGDPKGVM
Sbjct: 183 GGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVM 242

Query: 242 ISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRG 301
           ISN+SI+++  GV   L NVN  L+EKDVY+SYLPLAH+FDR IEE  I  G SIGFWRG
Sbjct: 243 ISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRG 302

Query: 302 DVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRKG 361
           DVKLL+ED+GELKPSIFCAVPRVLDR+Y GL QKL  GG FKK +F++A+S+KF NM+KG
Sbjct: 303 DVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKG 362

Query: 362 HKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTE 421
             H  ASP CDK+VF+KVK+GLGGNVR+ILSGAAPLA+H+E+FLRVVAC +VLQGYGLTE
Sbjct: 363 QSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTE 422

Query: 422 TCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGY 481
           +CAGTF + P+EL MLGTVGPPVPNVD+ LESVPEM YDAL +TPRGEIC+RG  LFSGY
Sbjct: 423 SCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGY 482

Query: 482 YKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLV 541
           YKR+DLT EV IDGW HTGD+GEWQP+GS+KIIDRKKNIFKL+QGEYVAVENLEN+Y  V
Sbjct: 483 YKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQV 542

Query: 542 SSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELNK 601
             ++ IWVYGNSFESFLVA+ANP +  LERWA EN + GDF+S+C++ +AK ++LGEL K
Sbjct: 543 EVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVK 602

Query: 602 IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKK 660
            AKE KLKGFE IK +HL+P+ FDMERDL+TPTYKKKRPQLLKYYQNVI  MY  TK+
Sbjct: 603 TAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQNVIHEMYKTTKE 660

BLAST of Tan0003704 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1010.4 bits (2611), Expect = 6.8e-295
Identity = 470/660 (71.21%), Postives = 561/660 (85.00%), Query Frame = 0

Query: 1   MSQMKYLLEVEKPKDAID-GRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPG 60
           M+  +Y++EVEK K  +D G PS+GPVYRSI+AKDGFP P D L S WDIFRLSVE++P 
Sbjct: 1   MATGRYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPN 60

Query: 61  NRMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIIS 120
           N MLGRREIV+GKAGKYVW TYKEV+++VIK+GNS+   G  +G KCGIYGAN  EWIIS
Sbjct: 61  NPMLGRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIIS 120

Query: 121 MEACNAHGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIV 180
           MEACNAHGLYCVPLYDTLGAGA+EFIICH+E+S+AF EE K+ E+LKT P +TKYLK IV
Sbjct: 121 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIV 180

Query: 181 SFGKVTPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKG 240
           SFG+VT N R E E++ L I+SWD+FL LGE +H++LP K++SD+ TIMYTSGTTGDPKG
Sbjct: 181 SFGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKG 240

Query: 241 VMISNKSIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFW 300
           V+++N+SII L+ GV++LL+ ++EEL  KDVYLSYLPLAHIFDRVIEEL I   ASIGFW
Sbjct: 241 VLLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFW 300

Query: 301 RGDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMR 360
           RGDVK+L+EDI  LKP++FCAVPRVL+RIY GL QKL  GG  KK LFN A+ +K  NM 
Sbjct: 301 RGDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNME 360

Query: 361 KGHKHGEASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGL 420
           KG  H +ASPI DKIVF KVKEGLGGNVRLILSGAAPLA H+E+FLRVVACAHVLQGYGL
Sbjct: 361 KGQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGL 420

Query: 421 TETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFS 480
           TE+C GTFVS+PNELSMLGTVGPPVPNVD+ LESVPEMGYDAL++ PRGEIC+RG  LFS
Sbjct: 421 TESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFS 480

Query: 481 GYYKRDDLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 540
           GYYKR+DLT EV IDGW HTGD+GEWQPDG++KIIDRKKNIFKLSQGEYVAVENLENIY 
Sbjct: 481 GYYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYS 540

Query: 541 LVSSLDMIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGEL 600
            V++++ IWVYGNS+ES+LVAV  P+K+ +E WA E+ + GDF+S+C +++ K+++LGE 
Sbjct: 541 HVAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEF 600

Query: 601 NKIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKK 660
           N++AK+KKLKGFE IK +HLD +PFDMERDLITP+YK KRPQLLKYYQ  ID MY   ++
Sbjct: 601 NRVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEIDEMYKKNRE 660

BLAST of Tan0003704 vs. TAIR 10
Match: AT1G49430.1 (long-chain acyl-CoA synthetase 2 )

HSP 1 Score: 758.1 bits (1956), Expect = 6.1e-219
Identity = 353/655 (53.89%), Postives = 478/655 (72.98%), Query Frame = 0

Query: 7   LLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGNRMLGRR 66
           +L VE+ + A    PS GPVYR  +AKDG       +DS W  F  +V++ P  +MLG+R
Sbjct: 8   VLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQR 67

Query: 67  EIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISMEACNAH 126
              + K G Y W+TYKE +D  I+IG+++ +RG + G  CGIYGANC EWII+MEAC + 
Sbjct: 68  VTTDSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQ 127

Query: 127 GLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVSFGKVTP 186
           G+  VPLYD+LG  AVEFII H+E+S+ FV+EK +  +L      +  LKTIVSFG+V+ 
Sbjct: 128 GITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSS 187

Query: 187 NLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMISNKS 246
             +EE +   + +FSW+EF L+G     +LP K+K+DI TIMYTSGTTG+PKGV+++N +
Sbjct: 188 TQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAA 247

Query: 247 IISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKLL 306
           I   +  + ++LE  +   +  DV+ SYLPLAH +D+V+E  F+  G+S+G+WRGD++ L
Sbjct: 248 ISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYL 307

Query: 307 VEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRKGHKHGE 366
           ++D+  LKP++FC VPRV D++YAG+ QK+ A G+ +K LF+ AY++K  NMRKG    E
Sbjct: 308 MDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEE 367

Query: 367 ASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTETCAGT 426
           ASP  D+++F K+KE LGG   ++LSGAAPL  HVE FLR++  +++ QGYGLTE+C G+
Sbjct: 368 ASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGS 427

Query: 427 FVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALST-TPRGEICVRGDPLFSGYYKRD 486
           F +L    SM+GTVG P+P V+  L SVPEMGYDA S   PRGEIC+RG+ +FSGY+KR 
Sbjct: 428 FTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQ 487

Query: 487 DLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVSSLD 546
           DLT++V+IDGWFHTGDIGEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLEN Y     + 
Sbjct: 488 DLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 547

Query: 547 MIWVYGNSFESFLVAVANPNKLALERWANEN-DIEGDFDSLCEDKRAKDYLLGELNKIAK 606
            IWVYGNSFESFLV V  P++ A+E WA  N     DF+SLC++ +A+ Y L ELN  AK
Sbjct: 548 QIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNSTAK 607

Query: 607 EKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMYNNTKK 660
           + +LKGFE +KAIHL+P PFD+ERDLITPT+K KRPQLL++Y+ ++D++Y+  K+
Sbjct: 608 QYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYKGIVDQLYSEAKR 662

BLAST of Tan0003704 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 674.9 bits (1740), Expect = 6.7e-194
Identity = 320/649 (49.31%), Postives = 444/649 (68.41%), Query Frame = 0

Query: 6   YLLEVEKPKDAIDGRPSIGPVYRSIFAKDGFPPPIDGLDSCWDIFRLSVERNPGNRMLGR 65
           +  +VE+    IDG+PS+GPVYR++ ++ GFPP    + + WDIF  SVE+ P N MLG 
Sbjct: 4   FAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNMLGW 63

Query: 66  REIVNGKAGKYVWLTYKEVYDMVIKIGNSLHNRGFEEGAKCGIYGANCSEWIISMEACNA 125
           R IV+ K G Y+W TYKEVY+ V++IG++L   G E G++ GIYG NC +WII+MEAC A
Sbjct: 64  RRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEACAA 123

Query: 126 HGLYCVPLYDTLGAGAVEFIICHSEISIAFVEEKKLPEVLKTFPNTTKYLKTIVSFGKVT 185
           H L CVPLYDTLG+GAV++I+ H+EI   FV++ K+  +L+      K LK IVSF  V+
Sbjct: 124 HTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFTNVS 183

Query: 186 PNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGVMISNK 245
             L  +  + G++ +SW +FL +G  +  D    K  +I TIMYTSGT+GDPKGV+++++
Sbjct: 184 DELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVLTHQ 243

Query: 246 SIISLIAGVQRLLENVNEELNEKDVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKL 305
           ++ + + G+   ++   +++   DVYLS+LPLAHI DR+ EE F   GAS+G++ G++ +
Sbjct: 244 AVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGNLNV 303

Query: 306 LVEDIGELKPSIFCAVPRVLDRIYAGLTQKLDAGGIFKKTLFNLAYSHKFSNMRKGHKHG 365
           L +DI ELKP+    VPRV +RI+ G+ + L      ++ +FN  Y HK + + +G+ H 
Sbjct: 304 LRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGYSHS 363

Query: 366 EASPICDKIVFSKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLTETCAG 425
           +ASP+ D I F K+++ LGG +RL++SG APL+  +E FLRV  C  V+QGYGLTET  G
Sbjct: 364 KASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTETLGG 423

Query: 426 TFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYYKRD 485
           T +  P+E+ MLGTVG P    ++ LE V EMGYD L   P GEIC+RG  +FSGYYK  
Sbjct: 424 TALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYYKNP 483

Query: 486 DLTNEVMIDGWFHTGDIGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGLVSSLD 545
           +LT EVM DGWFHTGDIGE  P+G LKIIDRKKN+ KLSQGEYVA+E+LENI+G  S + 
Sbjct: 484 ELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNSVVQ 543

Query: 546 MIWVYGNSFESFLVAVANPNKLALERWANENDIEGDFDSLCEDKRAKDYLLGELNKIAKE 605
            IWVYG+SF+S LVAV  PN   + RWA +      F+ LC     K++++ EL   A++
Sbjct: 544 DIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKSTAEK 603

Query: 606 KKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQNVIDRMY 655
            KL+ FE+IKA+ ++  PFD+ERDL+T T K +R  LLKYYQ  ID MY
Sbjct: 604 NKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQIDEMY 652